Title:
ADENOVIRUS VECTORS COMPRISING INTRONS
Document Type and Number:
WIPO Patent Application WO2002006502
Kind Code:
A3
Abstract:
The invention disclosed herein provides adenovirus vectors comprising introns 5' to transgenes. Such adenovirus vectors are useful for expression of proteins. Methods of making and using these adenovirus vectors are provided herein. In particular, methods of obtaining increased expression of viral proteins from the adenovirus vectors comprising introns 5' to transgenes is provided.
Inventors:
TIKOO SURESH K (CA)
Application Number:
PCT/IB2001/001537
Publication Date:
May 16, 2002
Filing Date:
July 13, 2001
Export Citation:
Assignee:
UNIV SASKATCHEWAN (CA)
TIKOO SURESH K (CA)
TIKOO SURESH K (CA)
International Classes:
C12N15/09; A61K35/76; A61K39/00; A61K48/00; A61P31/00; A61P31/04; A61P31/14; A61P31/20; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N7/00; C12N15/861; (IPC1-7): C12N15/86; A61K48/00
Domestic Patent References:
| WO2001018224A1 | 2001-03-15 | |||
| WO2001002607A1 | 2001-01-11 | |||
| WO1999061638A1 | 1999-12-02 |
Foreign References:
| FR2642767A1 | 1990-08-10 |
Other References:
REDDY P SESHIDHAR ET AL: "Optimization of bovine coronavirus hemagglutinin-estrase glycoprotein expression in E3 deleted bovine adenovirus-3.", VIRUS RESEARCH, vol. 70, no. 1-2, September 2000 (2000-09-01), pages 65 - 73, XP001066302, ISSN: 0168-1702
MATTHEWS ET AL: "Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein", JOURNAL OF GENERAL VIROLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 80, no. PART 2, February 1999 (1999-02-01), pages 345 - 353, XP002130777, ISSN: 0022-1317
MUKHOPADHYAY K ET AL: "Use of a new rat chondrosarcoma cell line to delineate a 119-base pair chondrocyte-specific enhancer element and to define active promoter segments in the mouse pro-alpha 1(II) collagen gene", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 270, no. 46, 17 November 1995 (1995-11-17), pages 27711 - 27719, XP002154470, ISSN: 0021-9258
FREDERICKSON R M ET AL: "5' FLANKING AND FIRST INTRON SEQUENCES OF THE HUMAN SS-ACTIN GENE REQUIRED FOR EFFICIENT PROMOTER ACTIVITY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 17, no. 1, 11 January 1989 (1989-01-11), pages 253 - 270, XP000008470, ISSN: 0305-1048
YUAN W: "Intron 1 rather than 5' flanking sequence mediates cell type-specific expression of c-myb at level of transcription elongation", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1490, no. 1-2, 31 January 2000 (2000-01-31), pages 74 - 86, XP004275570, ISSN: 0167-4781
RAPER S E ET AL: "SELECTIVE GENE TRANSFER INTO THE LIVER OF NON-HUMAN PRIMATES WITH EI-DELETED, E2A-DEFECTIVE, OR E1-E4 DELETED RECOMBINANT ADENOVIRUSES", HUMAN GENE THERAPY, vol. 9, 20 March 1998 (1998-03-20), pages 671 - 679, XP000995307, ISSN: 1043-0342
DEDIEU J-F ET AL: "LONG-TERM GENE DELIVERY INTO THE LIVERS OF IMMUNOCOMPETENT MICE WITH E1/E4-DEFECTIVE ADENOVIRUSES", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 71, no. 6, 1 June 1997 (1997-06-01), pages 4626 - 4637, XP000619915, ISSN: 0022-538X
MATTHEWS ET AL: "Development and use of a 293 cell line expressing lac repressor for the rescue of recombinant adenoviruses expressing high levels of rabies virus glycoprotein", JOURNAL OF GENERAL VIROLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 80, no. PART 2, February 1999 (1999-02-01), pages 345 - 353, XP002130777, ISSN: 0022-1317
MUKHOPADHYAY K ET AL: "Use of a new rat chondrosarcoma cell line to delineate a 119-base pair chondrocyte-specific enhancer element and to define active promoter segments in the mouse pro-alpha 1(II) collagen gene", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 270, no. 46, 17 November 1995 (1995-11-17), pages 27711 - 27719, XP002154470, ISSN: 0021-9258
FREDERICKSON R M ET AL: "5' FLANKING AND FIRST INTRON SEQUENCES OF THE HUMAN SS-ACTIN GENE REQUIRED FOR EFFICIENT PROMOTER ACTIVITY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 17, no. 1, 11 January 1989 (1989-01-11), pages 253 - 270, XP000008470, ISSN: 0305-1048
YUAN W: "Intron 1 rather than 5' flanking sequence mediates cell type-specific expression of c-myb at level of transcription elongation", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1490, no. 1-2, 31 January 2000 (2000-01-31), pages 74 - 86, XP004275570, ISSN: 0167-4781
RAPER S E ET AL: "SELECTIVE GENE TRANSFER INTO THE LIVER OF NON-HUMAN PRIMATES WITH EI-DELETED, E2A-DEFECTIVE, OR E1-E4 DELETED RECOMBINANT ADENOVIRUSES", HUMAN GENE THERAPY, vol. 9, 20 March 1998 (1998-03-20), pages 671 - 679, XP000995307, ISSN: 1043-0342
DEDIEU J-F ET AL: "LONG-TERM GENE DELIVERY INTO THE LIVERS OF IMMUNOCOMPETENT MICE WITH E1/E4-DEFECTIVE ADENOVIRUSES", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 71, no. 6, 1 June 1997 (1997-06-01), pages 4626 - 4637, XP000619915, ISSN: 0022-538X
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