ALIPHATIC AMINO ACID LABELED WITH STABLE ISOTOPE, METHOD OF INSERTING THE SAME IN TARGET PROTEIN, AND METHOD OF NMR CONFORMATION ANALYSIS OF PROTEIN

WIPO Patent Application WO/2007/099934

A1

A combination of aliphatic amino acids labeled with stable isotope for realizing of an analysis of the conformation of protein of high molecular weight, especially molecular weight as high as exceeding 60 kDa. There is provided a combination of amino acids labeled with stable isotope characterized in that arginine (Arg), glutamine (Gln), glutamic acid (Glu), lysine (Lys), methionine (Met) and proline (Pro) satisfy the following label patterns: (b) one of methylene hydrogens of methylene group at one or more positions is deuterized, and both of two methylene hydrogens of methylene group at one or more positions are deuterized; and (d) when a methyl group exists, one hydrogen of the methyl group is left intact and the other hydrogens are deuterized, or alternatively the methyl group is wholly deuterized.

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KAINOSHO MASATSUNE (JP)
TERAUCHI TSUTOMU (JP)

PCT/JP2007/053593

September 07, 2007

February 27, 2007

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JAPAN SCIENCE & TECH AGENCY (JP)
KAINOSHO MASATSUNE (JP)
TERAUCHI TSUTOMU (JP)

C07C229/24; C07B59/00; C07C229/26; C07C231/02; C07C279/14; C07C323/58; C07D207/16; C07D209/20; C07D233/64; C07K1/13; C12P21/02; G01R33/465

WO2005042469A1	2005-05-12
WO2003053910A1	2003-07-03

KUMAKURA, Yoshio et al. (Shin-Tokyo Bldg. 3-1, Marunouchi 3-chom, Chiyoda-ku Tokyo 55, JP)

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