Title:
ALK GENE AND EML4 GENE DETECTION PROBE, PREPARATION METHOD THEREFOR, AND REAGENT KIT
Document Type and Number:
WIPO Patent Application WO/2017/114003
Kind Code:
A1
Abstract:
Disclosed in the present invention are an ALK gene detection probe, and an ALK gene and EML4 gene detection probe, and a preparation method therefor. The method comprises the following steps: selecting at least one of RP11-62B19, RP11-100C1 and CTD-2245E6 as a BAC clone for an ALK gene, and selecting at least one of CTD-2544I11, RP11-684O3 and RP11-134O13 as a BAC clone; selecting at least one of CTD-2358L8 and CTD-2547J15 as a BAC clone for an EML4 gene; obtaining a plasmid DNA; and performing labeling. Also disclosed in the present invention is a reagent kit comprising the ALK gene and EML4 gene detection probe.
Inventors:
HE GUI (CN)
CHEN SHAOYU (CN)
OU HUANJIN (CN)
ZHANG HUIQING (CN)
CHEN SHAOYU (CN)
OU HUANJIN (CN)
ZHANG HUIQING (CN)
Application Number:
PCT/CN2016/105703
Publication Date:
July 06, 2017
Filing Date:
November 14, 2016
Export Citation:
Assignee:
GUANGZHOU LBP MEDICINE SCIENCE AND TECH CO LTD (CN)
International Classes:
C12Q1/68; C12N15/10; C12N15/11
Domestic Patent References:
| WO2009102446A2 | 2009-08-20 |
Foreign References:
| CN105543353A | 2016-05-04 |
Other References:
DEBELENKO L.V. ET AL.: "Renal cell carcinoma with novel VCL-ALK fusion: new representative of ALK-associated tumor spectrum", MODERN PATHOLOGY, vol. 24, no. 3, 31 March 2011 (2011-03-31), pages 430 - 442, XP055215238
JOKOJI R ET AL.: "Combination of morphological feature analysis and immunohistochemistry is useful for screening of EML4-ALK-positive lung adenocarcinoma", J CLIN PATHOL, vol. 63, 9 October 2010 (2010-10-09), pages 1066 - 1070
JOKOJI R ET AL.: "Combination of morphological feature analysis and immunohistochemistry is useful for screening of EML4-ALK-positive lung adenocarcinoma", J CLIN PATHOL, vol. 63, 9 October 2010 (2010-10-09), pages 1066 - 1070
Attorney, Agent or Firm:
ADVANCE CHINA IP LAW OFFICE (CN)
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