Title:
ALZHEIMER-RESISTANT CELL MODEL INCLUDING APOE CHRISTCHURCH MUTATION AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/154343
Kind Code:
A1
Abstract:
The present invention relates to an Alzheimer-resistant human pluripotent stem cell model having an ApoE Christchurch mutation inserted thereinto and a construction method therefor and, more specifically, to an Alzheimer-resistant human induced pluripotent stem cell model and brain cell/brain organoid into which an ApoE Christchurch mutation is inserted using a CRISPR/Cas9 system, and a construction method therefor. According to the present invention, Alzheimer-resistant human induced pluripotent stem cells and a brain cell/brain organoid are constructed by inserting an ApoE Christchurch mutation into human induced pluripotent stem cells with the aid of a CRISPR/Cas9 gene editing technique and as such, can surmount the limitations observed in preexisting animal models, such as protein expression patterns different from those in human cells, etc. The Alzheimer-resistant cell model of the present invention can find advantageous applications in research into ApoE-associated Alzheimer pathogenesis, etc.
Inventors:
SEO JINSOO (KR)
BAE SIMSUNG (KR)
LEE HYEIN (KR)
JANG GEUM MI (KR)
CHO JOONHO (KR)
BAE SIMSUNG (KR)
LEE HYEIN (KR)
JANG GEUM MI (KR)
CHO JOONHO (KR)
Application Number:
PCT/KR2022/000012
Publication Date:
July 21, 2022
Filing Date:
January 10, 2022
Export Citation:
Assignee:
DAEGU GYEONGBUK INST SCIENCE & TECH (KR)
International Classes:
C12N5/079; C12N9/22; C12N15/10; C12N15/113; C12N15/90
Domestic Patent References:
| WO2019084958A1 | 2019-05-09 |
Foreign References:
| US20200095299A1 | 2020-03-26 | |||
| KR102170502B1 | 2020-10-28 | |||
| US20190338363A1 | 2019-11-07 |
Other References:
ARBOLEDA-VELASQUEZ JOSEPH F.; LOPERA FRANCISCO; O’HARE MICHAEL; DELGADO-TIRADO SANTIAGO; MARINO CLAUDIA; CHMIELEWSKA NATALIA: "Resistance to autosomal dominant Alzheimer’s disease in anChristchurch homozygote: a case report", NATURE MEDICINE, NATURE PUBLISHING GROUP US, NEW YORK, vol. 25, no. 11, 1 November 2019 (2019-11-01), New York, pages 1680 - 1683, XP036927379, ISSN: 1078-8956, DOI: 10.1038/s41591-019-0611-3
Attorney, Agent or Firm:
ISIS IP LAW LLC (KR)
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