Title:
AMIDE DERIVATIVE NEUTRAL MITOCHONDRIAL FLUORESCENT MARKERS, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/147872
Kind Code:
A1
Abstract:
The present invention relates to a series of amide derivative neutral mitochondrial fluorescent markers, a preparation method therefor, and an application thereof; the present invention discloses for the first time that, after being linked with an amide compound, neutral dye has an excellent mitochondrial targeting capability. The present invention solves the problem of the uncertainty of the organelle targeting capability of existing neutral structure fluorescent dyes and neutral dyes being commercial markers of lipid droplets in cells. The present invention, whilst improving the optical performance of a fluorescent dye, also regulates the organelle targeting capability of the original dye by means of an inventive modification to the structure thereof; after linkage with the amide compound, the biological properties of the dye can be significantly improved. Amide compounds are inexpensive and easy to obtain, which is beneficial for controlling the cost of new dyes, and have great scientific significance and commercial value.
Inventors:
GE JIANFENG (CN)
MA WEI (CN)
SUN RU (CN)
MA WEI (CN)
SUN RU (CN)
Application Number:
PCT/CN2021/075159
Publication Date:
July 14, 2022
Filing Date:
February 04, 2021
Export Citation:
Assignee:
UNIV SOOCHOW (CN)
International Classes:
C07D311/16; C07D265/38; C07D405/04; C07D413/04; C09K11/06; G01N21/64
Domestic Patent References:
| WO2019104199A1 | 2019-05-31 | |||
| WO2016171755A1 | 2016-10-27 |
Foreign References:
| JP2016138053A | 2016-08-04 |
Other References:
HARUKO TAKESHI, ET AL.: "Synthesis of 4-(7-Diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A Highly Sensitive Fluorescent Derivatization Reagent for Alcohols in High-Performance Liquid Chromatography [1]", J. HETEROCYCLIC CHEM., vol. 38, no. 2, 11 March 2009 (2009-03-11), pages 333 - 338, XP055949458, DOI: 10.1002/jhet.5570380202
LI HONGLIN: "Design of Turn-on Fluorescent Probes and the Application in Detection of Hydrogen Sulfide and pH", LIAONING UNIVERSITY MASTER'S THESIS, 東京, 28 February 2017 (2017-02-28), 東京, XP055949461, ISBN: 978-4-297-12813-5
XU YU, DAI XI, ZHAO BAO-XIANG: "A coumarin–indole based colorimetric and “turn on” fluorescent probe for cyanide", SPECTROCHIMICA ACTA PART A: MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, ELSEVIER, AMSTERDAM, NL, vol. 138, 1 March 2015 (2015-03-01), AMSTERDAM, NL, pages 164 - 168, XP055949464, ISSN: 1386-1425, DOI: 10.1016/j.saa.2014.11.013
LI HONGLIN: "Design of Turn-on Fluorescent Probes and the Application in Detection of Hydrogen Sulfide and pH", LIAONING UNIVERSITY MASTER'S THESIS, 東京, 28 February 2017 (2017-02-28), 東京, XP055949461, ISBN: 978-4-297-12813-5
XU YU, DAI XI, ZHAO BAO-XIANG: "A coumarin–indole based colorimetric and “turn on” fluorescent probe for cyanide", SPECTROCHIMICA ACTA PART A: MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, ELSEVIER, AMSTERDAM, NL, vol. 138, 1 March 2015 (2015-03-01), AMSTERDAM, NL, pages 164 - 168, XP055949464, ISSN: 1386-1425, DOI: 10.1016/j.saa.2014.11.013
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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