Description of the Prior Art Small peptides are excellent starting points for drug design because they have the potential to overcome the pharmacokinetic shortcomings of larger peptides, yet retain the desirable quality of molecular recognition. A number of dipeptides are currently being developed as novel pharmaceutical agents (see e. g., Blackburn et al., Bioorg. Med. Chem. Lett., 7: 823-26 (1997); Schullek et al., Anal. Biochem., 246: 20-29 (1997), each incorporated by reference herein). Unfortunately, even small peptides suffer from proteolytic instability which limits their use as drug candidates.

Peptide mimics have been developed that utilize the urea moiety as a non-hydrolyzable linker and/or a hydrogen bond acceptor. Further modifications to cyclic ureas have led to the generation of a new sub-class of biologically active compounds. A number of cyclic HIV protease inhibitors have been developed that incorporate ureas, sulfamides, and other urea surrogates as the central linchpin. In these cases, it has been shown that the H-bonding urea moieties may serve to replace the water molecule exclusive to the active site of HIV protease.

Ring-closing metathesis (RCM) reactions have become a highly effective strategy for the construction of a number of important heterocyclic compounds (see e. g., Fu et al., J 4m. Chem. Soc., 115: 9856 (1993), incorporated by reference herein) and constrained peptides (see e. g., Miller et al., R Am. Chem. Soc., 117: 5855-5856 (1995); Miller et al., J : Am. Chem. Soc., 118: 9606-9614 (1996); Blackwell et al., Angew. Chem., Int. Ed., 37: 3281-3284 (1998), each incorporated by reference herein).

Sulfamides are unique functional groups that have been exploited in the development of a number of novel pharmaceutical agents and synthetic ligands. Their ability to serve as urea surrogates have made them ideal functional groups for the development of novel peptidomi- metics, while their chelating ability has made them ideal for the development of novel ligands for asymmetric catalytic reactions. This diverse set of properties has made them important targets in the development of new chemical entities.

Since its discovery as the causative agent of AIDS, considerable effort has been placed on understanding the biomolecular replicative process of the human immunodeficiency virus (HIV), with primary focus being placed on the inhibition of a key virally encoded protease enzyme of the pol gene. Many synthetic approaches to the inhibition of HIV protease are based on the synthesis of peptidomimetics which replace a key scissile amide bond by a non- hydrolyzable transition state isostere. This strategy has been employed to synthesize a number of novel nonpeptidal HIV protease inhibitors. Among the more effective peptidomimetics, the synthesis of cyclic ureas (see e. g., Lucca et al., Drugs of the Future, 23: 987 (1998)), cyclic sulfamides (see e. g., Jadhav et al., Tetrahedron Lett., 36: 6383 (1995)), hydroxyethylene/hydroxyethylamine isosteres (see e. g., Thomas et al., Biorg. Med. Chem. Lett., 4: 2759 (1994)) have been reported.

SUMMARY OF THE INVENTION The present invention is broadly concerned with new sulfamide compounds and methods of forming such compounds. In more detail, the compounds are represented by a formula selected from the group consisting of

wherein: each X is individually selected from the group consisting of oxygen,-NH, and-NOR ; each R'is individually selected from the group consisting of hydrogen, branched and unbranched alkyl groups (preferably Cl-Clsn more preferably Cl-C8), branched and unbranched alkenyl groups (preferably C2-CI8, more preferably C2-C8), branched and unbranched alkynyl groups (preferably C2-C18, more preferably C2- C8), allyl groups, acyl groups (preferably Cl-Cl8, more preferably Cl-C8), aryl groups (preferably C6-CI2), 2-15 mer peptides, and benzyl groups; each R2 is individually selected from the group consisting of hydrogen, substituted and unsubstituted amino acid side chains, and 2-15 mer peptides; and each R3 is individually selected from the group consisting of hydrogen,-OH, and-NHR', with R1 being as defined above.

Preferably at least one W group comprises an amino acid side chain selected from the group consisting of wherein each R4 is individually selected from the group consisting of hydrogen, branched and unbranched alkyl groups (preferably Cl-Cl8, more preferably Cl-C8), branched and unbranched alkenyl groups (preferably C2-C18, more preferably C2-C8), branched and unbranched alkynyl groups (preferably C2-C18, more preferably C2-C8), allyl groups, aryl groups (preferably C6-C12), acyl groups (preferably C2-CI8, more preferably C2-C8), and benzyl groups.

Particularly preferred compounds according to the invention are those selected from the group consisting of

wherein each R2 is individually selected from the group consisting of-CH3, CH(CH3) 2,-CH2CH (CH3) 2, and-CH2Ph.

The inventive compounds are formed by reacting a template sulfamide compound which comprises an opened-ring structure (i. e., a partially-formed ring comprising at least half, but not all of the sides necessary to form a cyclic compound) with a ring-closing catalyst to yield the closed-ring phosphonamide compound. Preferred ring-closing catalysts are olefin metathesis catalysts such as Grubbs catalysts (see e. g., U. S. Patent Nos. 6,048,993,5,917,071,5,750,815, 5,710,298,5,342,909, and 5,312,940, each incorporated by reference herein) as well as those disclosed by the following references, each also incorporated by reference herein: Matthias, Org.

Ltrs., 1 (6): 953-56 (1999); Schrock, Macromolecules, 29 (19): 6114-25 (1996); Zhu et al., J. Amer.

Chem. Soc., 121 (36): 8251-59 (1999); Alexander et al., J Amer. Chem. Soc., 120 (16): 4041-42 (1998); and Kingsbury et al., J : Amer. Chem. Soc., 121 (4): 791-99 (1999).

Particularly preferred Grubbs catalysts are those selected from the group consisting of

Preferred template structures comprise an allylated sulfamide, with particularly preferred template structures being those selected from the group consisting of wherein: each X is individually selected from the group consisting of oxygen,-NH, and-NOR' ; each R'is individually selected from the group consisting of hydrogen, branched and unbranched alkyl groups (preferably Cl-Cl8, more preferably Cl-C8), branched

and unbranched alkenyl groups (preferably C2-CI8, more preferably C2-C8), branched and unbranched alkynyl groups (preferably C2-CI8, more preferably C2- C8), allyl groups, acyl groups (preferably Cl-Cl8, more preferably Cl-C8) aryl groups (preferably C6 C12), 2-15 mer peptides, and benzyl groups; and each W is individually selected from the group consisting of hydrogen, substituted and unsubstituted amino acid side chains, and 2-15 mer peptides.

Preferably the reacting step is carried out at a temperature of from about 15-80 °C, and more preferably from about 30-55 °C. Furthermore, the reacting step should be carried out in a solvent system comprising a solvent selected from the group consisting of toluene, benzene, chlorobenzene, dichlorobenzene, methylene chloride, dimethoxyethane, methanol, water, and mixtures thereof. Preparing the ringed sulfamide compounds according to the inventive methods should result in a yield of those compounds of at least about 70%, and preferably at least about 90%, wherein the theoretical yield is taken as 100%.

It will be appreciated that the inventive methods allow for the synthesis of a wide array of both symmetric and unsymmetric cyclic sulfamide compounds. Furthermore, the inventive methods allow for preparation of, or selection of, templates having particular functional groups bonded thereto which are then readily formed into the desired sulfamide in a controlled and repeatable manner. Because the method can be adapted to form sulfamide compounds comprising one or more amino acid side chains or peptides bonded thereto, the inventive compounds can be used to inhibit HIV proteases, carbonic anhydrase, renin, and other enzymes.

The inventive compounds may also be used as retroviral inhibitors, anti-inflammatory agents, bioadhesion inhibitors, and herbicides.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS EXAMPLES The following examples set forth preferred methods in accordance with the invention.

It is to be understood, however, that these examples are provided by way of illustration and nothing therein should be taken as a limitation upon the overall scope of the invention.

A number of abbreviations are used herein. These abbreviations and the term or terms that they represent are set forth in Table A.

Table A

Abbreviation Term (s) hex hexane Bn benzyl Ph phenyl Me methyl Et ethyl Boc butoxy carbonyl EtOAc ethyl acetate Et3N triethyl amine tBuO tert-butoxy Grubbs Catalysts were used in some of the following Examples. These catalysts are referred to as follows: Grubbs Catalyst 1 Grubbs Catalyst 2 Grubbs Catalyst 3 EXAMPLE 1 The overall reaction scheme followed in Examples lA-1 C below is set forth in Scheme A.

Scheme A

le o o R' R S02C12 Et3N MeO2C N N) C02Me C1H3N CO2Me (70-85%) H H 8a-d 7a-d K2CO3, CH3CN or (94-98%) CH3COCH3 Br O O R2 2 Is, Grubbs Catalyst 1 Me02C N N C02Me- ; CH CH2C12 (94-98%) 4a-d 4a-d 9a-d a: R2 = CH3 b: R2 = CH (CH3) 2 c: R2=CH2CH (CH3) 2 d: R2=CH2Ph EXAMPLE 1A In the following parts I-IV of this example, compounds 7a-7d were used to prepare compounds 8a-8d, respectively. Scheme B depicts these compounds and the reaction in this portion. In this and the following procedure descriptions, the number/letter abbreviation depicted in the particular reaction scheme follows the chemical name of the particular compound (e. g., " (8b)" follows"N, N'-Sulfonyl bis-L-valine dimethyl ester").

Scheme B

7a: R2 = CH3 7b: R2=CH(CH3)2 7c : R2=CH2CH(CH3)2 7d: R2=CH2Ph 8a: R2=CH3 8b: R2=CH(CH3)2 8c : R2=CH2CH(CH3)2 8d : R2=CH2Ph I. Preparation ofN, N'-Sulfonyl bis-L-valine dimethyl ester (8b).

H-Val-OMeHCl (7b) (2.61 g, 15.56 mmol) and CH2Cl2 (85 mL) were added sequentially to a 250 mL round-bottom flask. The solution was cooled to 0°C, Et3N (4.50 g, 44.5 mmol) added slowly, and the resulting solution was stirred for 15 min. SO2Cl2 (595 pL, 1.00 g, 7.41 mmol) was added slowly over 45 minutes, and the resulting yellow solution was then warmed to room temperature over 3 hours. The solvent was concentrated to 15 mL under reduced pressure, EtOAc (225 mL) was added, and the solution was washed twice with 10% NaHSO4, aqueous NaHC03, brine, and dried MgSO4). The solution was filtered and concentrated under reduced pressure to leave a crude oil. Flash chromatography (SiO2, Hexanes/EtOAc) afforded 1.98 g (82.6%) of sulfamide (8b) as a white solid. mp = 76-77°C ; TLC Rf = 0.58 (2: 1 Hexanes/EtOAc); [a] 25D = +14.2 (c = 1. 02, CHCl3) ;'H NMR (CDCl3, 400 MHZ) 8 5.01 (d, J= 9.6 Hz, 2H), 3.88 (dd, J= 9.6,4.4 Hz, 2H), 3.77 (s, 6H), 2.18-2.10 (m, 2H), 1.00 (d, J= 6.9 Hz, 6H), 0.88 (d, J= 6.9 Hz, 6H) ; 13C NMR (CDCl3, 100 MHZ) 5 172. 9,61.1,52.4,31.5,18.8,17.4; FTIR (neat) 3317,3266,1737,1466,1355,1327,1137 cm-' ; HRMS (M+H) + calculated for Cl2H25N206S 325.1437, found 325.1432. ii Preparation of N, N'-Sulfonyl bis-L-alanine dimethyl ester (8a) In a procedure similar to the preparation of sulfamide (8b), H-Ala-OMe#HCl (2.18 g, 15.56 mmol) was subjected to sulfamide formation conditions (SO2Cl2, Et3N, CH2C12 0 °C, room temperature, 2hours). Flash chromatography (Si02, Hexanes/EtOAc) yielded 617 mg (31%) of

sulfamide (8a) as a white solid. mp = 91-92 °C ; TLC Rf = 0.40 in (2: 1 Hexanes/EtOAc); [a] 25D =-75. 8 (c = 1.003, CHC13) ;'H NMR (CDCl3, 400 MHZ) 8 5.24 (d, J= 8.0 Hz, 2H), 4.09 (dq, J= 7.3.7.3 Hz, 2H), 3.75 (s, 6H), 1.43 (d, J= 7.2 Hz, 6H) ; 13C NMR (CDC13, 100 MHZ) 8 173.7,52.7,51.8,19.2; FTIR (neat) 3273,1734,1457,1378,1353,1137 cl-n-1 ; HRMS (M+H) + calculated for C8HI7N206S 269.0807, found 269.0791.

III. Preparation ofN, N'-Sulfonyl bis-L-leucine dimethyl ester (8c) andN, N'-Sulfonyl bis-L- phenylalanine dimethyl ester (8d) N, N-Sulfonyl bis-L-leucine dimethyl ester (8c) and N, N'-Sulfonyl bis-L-phenylalanine dimethyl ester (8d) were prepared according to the procedure described by Sowada, J Prakt.

Chem. 20: 310 (1963) and McDermott et al., Org. Prep. Proc. Int. 16: 49-77 (1984), each being incorporated by reference herein.

IM Preparation of N,N'-Sulfonyl bis[(R)-1-phenyl-ethylamine] (8e) In a procedure similar to the preparation of sulfamide (8b), (R)-phenethylamine (471 mg, 501 µL, 3.84 mmol) was subjected to sulfamide formation conditions (SO2C12, Et3N, CH2cl2 0°C-RT, 8 h). Flash chromatography (SiO2, Hexanes/EtOAc) yielded 356 mg (63%) of sulfamide (8e) as a white solid. mp = 96-97°C ; TLC Rf= 0.37 (3: 1 Hexanes/EtOAc) ; [a] 25D= -31.2 (c = 1.02, CHC13) ;'H NMR (CDC'3, 400 MHZ) 8 7.27-7.21 (m, 6H), 7.17-7.14 (m, 4H), 4.74 (s, 2H), 4.43 (dq, J= 12.2.6.2 Hz, 2H), 1.46 (d, J= 6.8 Hz, 6H) ; 13C NMR (CDCl3, 100 MHZ) 6 142. 7,128.6,127.5,126.1,53.7,23.6; FTIR (neat) 3312, 3032,2992,2944,1603,1493, 1454,1381,1320,1149,751,701 cm-1 ; HRMS (M+H)+calculated for C16H21N2O2S 305. 1324, found 305.1322.

In Parts I-IV of this procedure, methylene chloride was the solvent utilized. However, acetonitrile, chloroform, toluene, benzene, chlorobenzene, dichlorobenzene, tetrahydrofuran (THF), diethyl ether, acetone, dimethoxyethane (DME), and mixtures thereof are also suitable solvents. Furthermore, while Et3N was used as the base, other bases which could be used included pyridine, NaHCO3, Na2CO3, K2CO3, NaH, KH, any tertiary amine, and mixtures thereof.

Finally, while the procedure was carried out at temperatures of 0-20°C, temperatures of anywhere from about-20-20°C would also be suitable.

EXAMPLE 1B In the following parts I-V of this example, compounds 8a-8e were used to prepare compounds 9a-9e, respectively. Scheme C depicts these compounds and the reactions.

Scheme C 8b : R'= CH (CH3) 2 8c : R2 =CH2CH (CH3) 2 8d: R2 = CH2Ph 9a: R2=CH3 9b: R2 = CH (CH3) z 9e : R2=CH2CH(CH3)2 9d: R2 = CH2Ph or 8e: R2 = Ph 9e: R2 = Ph I. Preparation of N,N'-Bis(2-propenyl)-N,N'-sulfonyl bis-L-valine dimethyl ester (9b) K2CO3 (1.06 g, 7.70 mmol) and allyl bromide were added to a stirring solution of sulfamide (8b) (500 mg, 1.54 mmol) in CH3CN (35 mL) in a 100 mL round-bottom flask (1.12 g, 9.24 mmol). The flask was fitted with a condenser, and the mixture was heated to 70°C for 14 hours. The resulting yellow orange mixture was filtered by suction, and the solvent was removed under reduced pressure to give a yellow oil. Flash Chromatography (SiO2, 10: 1 Hexanes/EtOAc) afforded 619 mg (98.7%) of sulfamide (9b) as a clear oil. TLC Rf= 0.31 (10: 1 Hexanes/EtOAc); [α]25D =-89.3 (c = 1. 00, CHC13) ;'H NMR (CDCl3, 400 MHZ) 5.92 (dddd, J = 15.9,10.2,7.5,5.7 Hz, 2H), 5.14 (dd, J= 16,1.3 Hz, 2H), 5.05 (dd, J= 10.2,1.2 Hz, 2H), 3.99-3.90 (m, 6H), 3.68 (s, 6H), 2.16-2.08 (m, 2H), 0.97 (d, J= 6.7 Hz, 6H), 0.86 (d, J= 6.6,

6H) ; 13C NMR (CDC13, 100 MHZ) 171.6,135.2,117.3,66.3,51.5,47.7,28.4,19.6,19.5 ; FTIR (neat) 3080,1745,1640,1436,1351,1137 cm-' ; HRMS (M+H) + calculated for C18H33N2O6S 405.2059, found 405.2031.

II. Preparation of N'-Bis(2-propenyl)-N,N'-sulfonyl bis-L-alanine dimethyl ester (9a) In a procedure similar to that used for the preparation of the allylated sulfamide (9b), sulfamide (8a) (500 mg, 1.86 mmol) was subjected to standard allylation procedures (K2CO3, allyl bromide, CH3CN 70°C, 18 hours). Flash chromatography (Si02, Hexanes/EtOAc) yielded 596 mg (92%) of sulfamide (9a) as a yellow oil. TLC Rf = 0.53 (3: 1 Hexanes/EtOAc); [a] 25D = -20.3 (c = 1. 004, CHCl3) ;'H NMR (CDC13, 400 MHZ) 5.87 (dddd, J=16. 8,10.5,6.5,6.3 Hz, 2H), 5.24 (dd, J= 15.9,1.4 Hz, 2H), 5.16 (dd, J= 10.2,1.2 Hz, 2H), 4.37 (q, J= 7.2 Hz, 2H), 3.98 (dddd, J=16. 1,6.1,1.1,1.1 Hz, 2H), 3.77 (dddd, J=16. 1,6.7,1.1,1.1 Hz, 2H) 3.72 (s, 6H), 1.47 (d, J= 4.2 Hz, 6H) ; 13C NMR (CDCl3, 100 MHZ) 172.0,134.4,118.0,55.0,52.0, 48.8,15.3; FTIR (neat) 1743,1645, 1333, 1226 cm-1; HRMS (M+H)+ calculated for C14H25N2O6S 349.1433, found 399.1436.

III. Preparation of N,N'-Bis(2-propenyl)-N,N'-sulfonyl bis-L-leucine dimethyl ester (9c) In a procedure similar to that used for the preparation of the allylated sulfamide (9b), sulfamide (8c) (2.08 g, 5.88 mmol) was subjected to standard allylation procedures (K2CO3, allyl bromide, refluxing acetone, 48 hours). Flash chromatography (SiO2, Hexanes/EtOAc) yielded 2.22 g (87%) of sulfamide (9c) as a yellow oil. TLC Rf = 0.64 (2: 1 Hexanes/EtOAc); [α]25D = -42.5 (c = 0. 51, CDC13) ; lH NMR (CDC13, 400 MHZ) 5.90 (dddd, J= 17. 2,10.2,6.8,6.1 Hz, 2H), 5.20 (dd, J=17. 2,1.3 Hz, 2H), 5.13 (dd, J= 10.2,1.1 Hz, 2H) 4.38 (dd, J= 8.2,6.0 Hz, 2H), 3.96 (dd, J= 16.2,6.0 Hz, 2H), 3.86 (dd, J=16. 2,7.0 Hz, 2H) 3.72 (s, 6H), 1.81-1.66 (m, 6H), 0.96 (d, J= 6.1 Hz, 6H), 0.91 (d, J= 6.2 Hz, 6H) 13C NMR (CDCl3, 100 MHZ) 172.4, 134.9,117.8,58.1,52.0,48.7,38.7,24.4,22.4,21.8; FTIR (neat) 3074,1746,1641,1344,1169 cm-1 ; HRMS (M+H)+ calculated for C2oH37N206S 433.2372, found 433.2397.

ITE Preparation of N,N'-Bis(2-propenyl)-N,N'-sulfonyl bis-L-phenyl alanine dimethyl ester (9d)

In a procedure similar to that used for the preparation of allylated sulfamide (9b), sulfamide (8d) (500 mg, 1.19 mmol) was subjected standard allylation procedures (K2C03, allyl bromide, CH3CN 70°C, 10 hours). Flash chromatography (SiO2, Hexanes/EtOAc) yielded 560 mg (95%) of sulfamide (9d) as a yellow oil. TLC Rf = 0.15 (10: 1 Hexanes/EtOAc); [a] 25D =- 47.9 (c =1.012, CHC13) ;'H NMR (CDC'3, 400 MHZ) 7.28-7.25 (m, 8H), 7.22-7.19 (m, 2H), 5.77 (dddd, J= 16.8,10.5,6.5,6.5 Hz, 2H), 5.17-5.13 (nfom, 4H), 4.51 (dd, J= 7.3,7.3 Hz, 2H), 3.71 (dd, J= 15.9,6.3 Hz, 2H), 3.66 (s, 6H), 3.58 (dd, J= 15. 9,6.9 Hz, 2H), 3.40 (dd, J= 14.1,7.6 Hz, 2H), 3.07 (dd, J= 14. 1,7.1 Hz, 2H) ; 13C NMR (CDCl3, 100 MHz) 171.0,137.3, 134.0,129.2,128.3,126.7,118.6,61.0,52.0,48.8,35.9; FTIR (neat) 3064,3029,1742,1653, 1559,1497,1456,1436,1339,1149,743,700 cnf' ; HPMS (M+H) + calculated for C26H33N206S 501.2059, found 501.2043.

Z Preparation of N,N'-Bis(2-propenyl)-N,N'-sulfonyl bis[(R)-1-phenyl-ethylamine] (9e) In a procedure similar to that used for the preparation of allylated sulfamide (9b), sulfamide (8e) (250 mg, 0.821 mmol) was subjected standard allylation procedures (K2C03, allyl bromide, CH3CN 70°C, 24 hours). Flash chromatography (SiO2, Hexanes/EtOAc) yielded 313 mg (99%) of sulfamide (9e) as a clear oil. TLC Rf = 0.59 (10: 1 Hexanes/EtOAc); [a] = =+77. 5 (c = 1.00, CHC13) ;'H NMR (CDCl3, 400 MHz) 7.46 (d, J= 7.4 Hz, 4H), 7.39-7.35 (m, 4H), 7.32-7.30 (m, 2H), 5.79 (dddd, J= 15. 7,9.7,7.1,5.8 Hz, 2H), 5.19 (q, J= 7.1 Hz, 2H), 5.03 (dd, J= 6.1,1. 3 Hz, 2H), 5.01-4.99 (m, 2H), 3.77 (dd with small allylic coupling, J=16. 3,5.8 Hz, 2H), 3.55 (dd, J=16. 3,7.2 Hz, 2H), 1.67 (d, J= 7.1 Hz, 6H) ;"C NMR (CDCl3, 100 MHz) 140.1,135.9,128.2,127.9,127.5,116.9,56.5,47.3,17.9; FTIR (neat) 3064,3030,2980,2937, 1640,1603,1496,1452,1378,1323,1161,784,699 cm-1 ; HRMS (M+H)+ calculated for C22H29N202S 385.1950, found 385.1958.

In Parts I-V of this procedure, acetone was the solvent utilized. However, acetonitrile, chloroform, toluene, benzene, chlorobenzene, dichlorobenzene, THF, diethyl ether, methylene chloride, DME, and mixtures thereof are also suitable solvents. Furthermore, while K2CO3 was used as the base, other bases which could be used included pyridine, NaHC03, Na2CO3, NaH, KH, and mixtures thereof. The allylating agent utilized was allyl bromide, but the following would work as well: allyl iodide; allyl bromide, NaI ; allyl bromide, KI; and allyl bromide,

Bu4NI. Finally, while the procedure was carried out at a temperature of 56°C or 82°C, temperatures of anywhere from about 40-100°C would also be suitable.

EXAMPLE 1C In the following parts I-V of this example, compounds 9a-9e were used to prepare compounds 4a-4e, respectively. Scheme D depicts these compounds and the reactions.

Scheme D RZ O p RZ R 0 0 RF MeO2CN \NCOMe z- GrubbsCatalyst 1 Me C N s \ N CO2Me 0 CH2CI2 (94-98%) 9a: R2=CH3 4a: R2 = CH3 9b: R2=CH(CH3)2 4b: R2=CH(CH3)2 9c : R2=CH2CH (CH3) 2 4c : R2=CH2CH(CH3)2 9d: R2=CH2Ph 4d: R2=CH2Ph or R'o oR' O p Ra S Me N N Me Grubbs Catalyst 1- In CECI2 (94-/.) 9e : R=Ph4e : R2=ph

I. Preparation of 2, 2'-(2S, 2'S)-[(2, 3, 6, 7-Tetrahydro-1, 2,7-thiadiazepine-1,1-dioxido-2,7- diyl) ]-3, 3'-dimethyl bis-butyric acid dimethyl ester (4b) A stirring solution of the allylated sulfamide (9b) (200 mg, 0.494 mmol) in CH2C12 (50 mL) in a 100 mL round-bottom flask was degassed by bubbling argon gas through the solution for 15 minutes. The Grubbs methathesis catalyst (1) (12 mg, 0.015 mmol, 3 mol%) was added, the flask was quickly fitted with a condenser containing an argon balloon, and the solution was heated to reflux for 1.5 hours. The solution was cooled to room temperature, and the flask opened to the air. CH2C12 (40 mL) and Celite' (5.0 g) were added, and the solution was stirred for 18 hours. The solvent was removed under reduced pressure, EtOAc (100 mL) was added, and the solution was filtered through a plug of silica. The solvent was again removed under reduced pressure to leave a crude solid. Flash chromatography (SiO2, Hexanes/EtOAc) gave 181 mg (97%) of the cyclic sulfamide (4b) as a clear solid. mp = 57-58°C ; TLC Rf = 0.11 (10: 1 Hexanes/EtOAc); [a] 25D =-114.7 (c = 1.00, CHC13) ; 1H NMR (CDCl3, 400 MHZ) 5.49 (t, J= 2.1 Hz, 2H), 4.17-4.12 (m, 4H), 3.65 (s, 6H), 3.65-3.60 (m, 2H), 2.17-2.11 (m, 2H), 1.02 (d, J = 6. 8 Hz, 6H), 0.91 (d, J= 6. 5 Hz, 6H) ;"C NMR (CDC13, 100 MHZ) 170.7,126.7,65.1,51.8, 40.4.27.3,19.1,18.8; FTIR (neat) 3031,1741,1436,1391,1370,1321,1137, cm-1 ; HRMS (M+H) + calculated for Cl6H29N206S 377.1746, found 377.1748.

II. Preparation of 2,2'-(2S,2'S)-[(2,3 6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-2,7- di bis-propionic acid dimethyl ester (4a) In a procedure similar to that used for the preparation of cyclic sulfamide (4b), allylated sulfamide (9a) (250 mg, 0.717 mmol) was subjected to ring-closing metathesis (RCM) conditions (reflux CH2C12, 3 mol% catalyst, 1.5 hours). Flash chromatography (SiO2, Hexanes/EtOAc) afforded 223 mg (97%) of cyclic sulfamide (4a) as a brown oil. TLC Rf = 0.19 (3: 1 Hex- anes/EtOAc); [a] 25D =-62.6 (c = 0.942, CHC13) ;'H NMR (CDCl3, 400 MHZ) 5.68 (t, J=1. 5 Hz, 2H), 4.76 (q, J= 7.3 Hz, 2H), 4.09 (d, J= 17.2 Hz, 2H), 3.73 (s, 6H), 3.68 (ddd, J= 20.1,3.2, 3.2 Hz, 2H) 1.41 (d, J= 7. 3 Hz, 6H) ;"C NMR (CDCl3, 100 MHZ) 172.0,127.8,55.3,52.3, 42.1,16.1; FTIR (neat) 2989,1741,1457,1313,1174 cm-' ; HRMS (M+H)+ calculated for Ct2H2lN206S 321.1120, found 321.1103.

III. Preparation of 2, 2'-(2S, 2'S)-[(2, 3, 6, 7-Tetrahydro-1, 2,7-thiadiazepine-1,1-dioxido-2,7- diyl)]- 4,4'-dimethyl bis-pentanoic acid dimethyl ester (4c) In a procedure similar to that used for the preparation of cyclic sulfamide (4b), allylated sulfamide (9c) (200 mg, 0.399 mmol) was subjected to RCM conditions (reflux CH2Cl2, 3 mol% catalyst, 15 hours). Flash chromatography (SiO2, Hexanes/EtOAc) and recrystallization (pentane/EtOAc) afforded 1.05 g (56%) of cyclic sulfamide (4c) as awhite solid. mp = 89-90 °C ; TLC Rf = 0.48 (2: 1 Hexanes/EtOAc); [a] 25D =-67.3 (c =1.06, CHCl3) ;'H NMR (CDCl3, 400 MHZ): 5.61 (m, 2H), 4.66 (dd, J= 7.9,6.8 Hz, 2H), 3.96-3.82 (m, 4H), 3.68 (s, 6H), 1.68-1.57 (m, 6H), 0.95 (d, J=6. 1 Hz, 6H), 0.91 (d, J=6. 3Hz, 6H) ; 13C NMR (CDCl3, 100 MHZ) : 171.8, 127.4,57.4,52.1,41.7,38.9,24.6,22.9,21.6; FTIR (neat) 3039,2959,2871,1741,1437,1373, 1181 cm'' ; HRMS (M+H) + calculated for C18H33N2O6S 405.2059, found 405.2033.

IE Preparation of 2,2'-(2S,2'S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-2,7- diyl)] 3,3'-diphenyl bis-propionic acid dimethyl ester (4d) In a procedure similar to that used for the preparation of cyclic sulfamide (4b), allylated sulfamide (9d) (200 mg, 0.399 mmol) was subjected to RCM conditions (reflux CH2C12, 3 mol% catalyst, 15 hours). Flash chromatography (SiO2, Hexanes/EtOAc) afforded 181 mg (96%) of cyclic sulfamide (4d) as a brown oil. TLC Rf = 0. 39 (3: 1 Hex/EtOAc); [a] 25D=-55. 2 (c =1. 098, CHC13) ;'H NMR (CDC13, 400 MHZ) 7.29-7.19 (m, 10H), 5.46 (t, J=1. 8 Hz, 2H), 4.91 (dd, J = 7.8,7.8 Hz, 2H), 3.65-3.63 (m, 10H), 3.21 (dd, J= 14.2,7.4 Hz, 2H), 2.89 (dd, J= 14.2,8.0 Hz, 2H) ; NMR (CDCl3, 100 MHZ) 170.7,136.0,129.2,128.2,127.3,126.8,60.3,52.1,41.9, 36.4; FTIR (neat) 3030,1739,1653,1604,1497,1356,1170,744,700 cm-l ; HRMS (M+H) + calculated for C24H29N206S 473.1746, found 473. 1751.

F Preparation of 1,1'-(1R,1'R)-[(2,3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-d ioxido-2, 7- diyl)]-l, l'-diphenyl ethane (4e) In a procedure similar to that used for the preparation of cyclic sulfamide (4b), allylated sulfamide (9e) (100 mg, 0.260 mmol) was subjected to RCM conditions (reflux CH2C12, 3 mol% catalyst, 16 hours). Flash chromatography (SiO2, Hexanes/EtOAc) afforded 887 mg (94%) of cyclic sulfamide (4e) as a white solid. mp = 90-92°C ; TLC Rf= 0.41 (10: 1 Hex/EtOAc); [a] 25D =-94. 3 (c = 0.70, CHCl3) ;'H NMR (CDCl3, 400 MHZ) 7.45 (d, J= 7.4 Hz, 4H), 7.39-7.35 (m,

4H), 7.30-7.27 (m, 2H), 5.51 (t, J= 1. 3 Hz, 2H), 5.44 (q, J= 7.0 Hz, 2H), 3.84 (d, J= 17 Hz, 2H), 3.54 (dd, J= 17.5,2.8 Hz, 2H), 1.57 (d, J= 7.1 Hz, 6H) ; 13C NMR (CDCl3, 100 MHZ) 140.5,128.4,128.0,127.4,127.3,56.1,40.8,17.7; FTIR (neat) 3029,2978,2931,1602,1496, 1451,1382, 1300,1177,785,698 cm-' ; HRMS (M+H) + calculated for C20H2sN202S 357. 1637, found 357.1616.

In Parts I-V of this procedure, methylene chloride was the solvent utilized. However, toluene, benzene, chlorobenzene, dichlorobenzene, DME, and mixtures thereof are also suitable solvents. Furthermore, while Grubbs catalyst 1 was utilized, Grubbs catalysts 2 and/or 3 would work as well. Finally, while the procedure was carried out at a temperature of 40°C, temperatures of anywhere from about 15-80°C would also be suitable.

EXAMPLE 1D In the following parts I-VII of this example, compounds 4a-4d were used to prepare various functionalized sulfamides. Scheme E depicts these compounds 4a-4d, the reaction schemes, and the resulting functionalized sulfamides.

Scheme E 4a-d a: R2=CH3 12a-d b: R2=CH (CH3) 2 c : R2=CH2CH (CH3) 2 d: R2 = CH2Ph I. Preparation of 2,2'-(2S,2'S)-[(2,3, 6, 7-Tetrahydro-1, 2, 7-thiadiazepine-1, 1-dioxido-2, 7- diyl) j-4, 4'-dimethyl bis-pentanol Lithium aluminum hydride (LAH) (11 mg, 0.273 mmol) was added to a stirring solution of the cyclic sulfamide (4c) (50 mg, 0.124 mmol) in THF (8 mL) in a 25 mL round-bottom flask fitted with Argon balloon at 0°C. The reaction was stirred for 4 hours with the addition of

another equivalent of LAH. Na2SO4#10H2O and several drops of distilled H2O were added until the stirred solid turned white. The mixture was filtered, and the solvent was removed under reduced pressure to give a clear oil. Purification by flash chromatography (SiO2, Hex- anes/EtOAc) gave 42 mg (98%) of the leucinol cyclic sulfamide as clear oil. TLC Rf= 0.34 (1: 2 Hexanes/EtOAc) ; [a] 25D =-6.8 (c = 0.78, CHC13) ; 1H NMR (CDCl3, 400 MHZ) 8 5.75 (t, J= 1.8 Hz, 2H), 4.15-4.08 (m, 2H), 3.86 (d, J= 17. 9 Hz, 2H), 3.70 (d, J= 17. 9 Hz, 2H), 3.58-3.49 (m, 4H), 2.37 (s, 2H), 1.68-1.59 (m, 2H), 1.38 (ddd, J= 14.3,9.5,4.8 Hz, 2H), 1.14 (ddd, J= 14.3,9.2,5.0 Hz, 2H), 0.94 (d, J= 6.5 Hz, 6H), 0.89 (d, J= 6.7 Hz, 2H) ; i3C NMR (CDC13, 100 MHZ) 8 128.8,64.3,59.5,40. 9,38.8,25.2,23.7,22.4; FTIR (neat) 3463,2955,2870,1468, 1379, 1290,1176 cm-1 ; HRMS (M+H) + calculated for Cl6H33N204S 349.2161, found 349.2170.

In this part of this procedure, THF was the solvent utilized. However, DME, ET20, methylene chloride, MeOH, EtOH, and mixtures thereof are also suitable solvents. Furthermore, while the reducing agent utilized was LAH, the following reducing agents would work as well: diisobutylaluminum hydride (DIBAL), A1H3, LiAlH2 (OCH2CH2OCH3) 2, LaAlH (OC (CH3) 3) 3, LiH3AlOtBu ; LiBH4 ; and NaBH4.

II. Preparation of 1,1'-bis(benzyloxy)-2,2'-(2S,2'S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine- 1, 1-dioxido-2, 7-diyl) J-4, 4'-dimethyl bis-pentane (10c) Sodium hydride (8 mg, 0.336 mmol) was added to a stirring solution of the leucinol sulfamide prepared in Part I of this example (39 mg, 0.112 mmol) in THF (10 mL) at 0 ° C. The mixture stirred for 45 min and benzyl bromide (57 mg, 0.336 mmol) was added. After stirring for 12 hours at room temperature, EtOAc (20mL) was added and the mixture was washed with 10% NaHSO4, NaHC03, brine, and dried (MgSO4). The solution was filtered and the solvent removed under reduced pressure. Purification by flash chromatography (SiO2, Hexanes/EtOAc) gave 48 mg (81%) of sulfamide (10c) as a clear oil. TLC Rf= 0. 78 (1: 2 Hexanes/EtOAc); [a] 25D =-11. 5 (c = 0.66, CHC13) ;'H NMR (CDC13, 400 MHz) 7.36-7.27 (m, 10H), 5.57 (s, 2H), 4.51 (d, J= 11. 9 Hz, 2H), 4.44 (d, J= 11. 9 Hz, 2H), 4.31 (m, 2H), 4.07 (d, J= 17. 3 Hz, 2H), 3.59 (dd, J= 19. 2,2.1 Hz, 2H), 3.46 (d, J= 5.4 Hz, 4H), 1.73-1.65 (m, 2H), 1.50 (ddd, J= 14. 3,10.0,4.6 Hz, 2H), 1.27 (dd, J= 14. 2,9.3,5.0 Hz, 2H), 0.98 (d, J= 6.5 Hz, 6H), 0.93 (d, J= 6.7 Hz, 6H); l3CNMR (CDC13, 100 MHz) 138. 1,128.3,127.9,127.6,127.5,72.9,72.5,55.5,40.4,38.5,24.6,

23.4,21.9 ; FTIR (neat) 3033,2955,2867,1496,1453,1386,1366,1301,1183,734,698 cm-' ; HRMS (M+H) + calculated for C30H45N204S 529.3100, found 529.3082.

In this part of this procedure, THF was the solvent utilized. However, methylene chloride, DME, Et2O, dimethyl formamide (DMF), and mixtures thereof are also suitable solvents. Furthermore, while BnBr and NaH were used as the benzylating agent and base, respectively, other suitable combinations include the following: BnBr with NaI, KH, and/or potassiumhexamethyldisilazide (KHMDS) or lauric diethanolamide (LDA); BnCI withNaI, KH, and/or KHMDS ; and CC13CN and KH with BnOH and tosic acid (TfOH).

III. Preparation of N, N'-dimethyl-[2, 2'-(2S, 2'S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine- 1, 1-dioxido-2, 7-diyl) JJ-4, 4'-dimethyl bis-pentanamide (Il c) A 10 mL round-bottom flask was charged with the leucine derived cyclic sulfamide (4c) (25 mg, 0.062 mmol) and MeNH2 (39 mg, 1. 24 mmol) in MeOH. The mixture was stirred under argon balloon for 5 days with the addition of two more equivalents of MeNH2. The solvent was removed, and the crude oil was purified by flash chromatography (SiO2, 4: 1 Hexanes/EtOAc) to give 21 mg (84%) of (Ilc) as a clear oil. TLC Rf= 0.23 (1 : 2 Hexanes/EtOAc); [a] 25D =-158. 5 (c = 0.26, CHC13) ;'H NMR (CDCl3, 400 MHZ) 6.41 (d, J= 3.8 Hz, 2H), 5.69-5.68 (m, 2H), 4.41 (dd, J= 9.1,5.4 Hz, 2H), 4.05 (d, J=16. 8 Hz, 2H), 3.61 (ddd, J= 17.0,4.5,1.1 Hz, 2H), 2.82 (d, J= 4.8 Hz, 6H), 1.84 (ddd, J= 14.2,8.8,5.4 Hz, 2H), 1.66-1.57 (m, 2H) 1.48 (ddd, J = 14.1,9.1,4.9 Hz, 2H), 0.95 (d, J= 6.5 Hz, 6H), 0.91 (d, J= 6.6 Hz, 6H) ;"C NMR (CDCl3, 100 MHZ) 170.8,127.4,59.2,42.4,38.2,26.6,24.8,23.0,21.7; FTIR (neat) 3381,3317,2958, 2870,1662,1544,1456,1368,1300,1179 cm-1 ; HRMS (M+H) + calculated for CIsH35N404S 403.2379, found 403.2406.

While MeNH2 and MeOH were utilized in this part of this procedure, other suitable combinations include: RINH2 and MeOH, RINH2 and EtOH ; and R1NH2 and isopropanol (iPrOH), acetonitrile, or acetone. In each of these instances R'can be hydrogen, branched and unbranched alkyl groups (preferably Cl-Cl8, more preferably Cl-C8), branched and unbranched alkenyl groups (preferably C2-CI8, more preferably C2-C8), branched and unbranched alkynyl groups (preferably C2-C18, more preferably C2-C8), allyl groups, acyl groups (preferably Cl-Cl8, more preferably Cl-C8), aryl groups (preferably C6-CI2), 2-15 mer peptides, and benzyl groups.

IVE Preparation of 2,2'-(2S,2'S)-[(2,3,6,7-Tetrahydro-1, 2,7-thiadiazepine-(4R), (5S)- dihydroxy-1,1-dioxido-2,7-diyl)]-4,4'-dimethyl bis-pentanoic acid dimethyl ester (12c) To a stirring solution of the cyclic sulfamide (4c) (50 mg, 0.123 mmol) in a mixture of acetone (3 mL) and distilled water (1 mL) was added N-methyl morpholine N-Oxide (32 mg, 0.248 mmol), and the mixture was stirred for 15 minutes. A 0.5 M aqueous solution of Os04 (1 mg, 4 umol) was then added. After 19 hours, Na2SO3 (50 mg, 0.31 mmol) was added to destroy the Os04. The mixture was filtered and concentrated to 1.5 mL under reduced pressure. Flash chromatography (Si02, 1: 2 Hexanes/EtOAc) gave 50 mg (92%) of (12c) as a clear oil. TLC Rf = 0.58 (1: 2 Hexanes/EtOAc); [a] 15 D =-21.9 (c = 0.64, CHC13) ; 1H NMR (CDCl3, 400 MHZ) 4.28 (dd, J= 9.3,5.8 Hz, 1H), 4.21 (dd, J= 10. 6,4.6 Hz, 1H), 3.92 (t, J=4. 6,1H), 3.83-3.73 (m, 2H), 3.79 (s, 3H), 3.76 (s, 3H), 3.65 (dd, J= 15.5,8.9 Hz, 1H), 3.40, (d, J= 12.4 Hz, 1H), 3.35 (dd, J= 15. 6,6.9 Hz, 1H), 1.97, (ddd, J= 13. 8, 10.3,3.3 Hz, 1H), 1.83-1.72 (m, 5H), 0.97 (d, J= 6.3 Hz, 3H), 0.97 (d, J= 6.6 Hz, 3H), 0.96 (d, J= 6.2 Hz, 3H), 0.94 (d, J= 6.3 Hz, 3H); 13C NMR (CDCl3, 100 MHZ) 174.2,173.8,69.4,69.0,61.6,59.2,53.1,52.8,48.8,46.1,39.2,38. 8,24.7, 24.7,23.2,22.9,21.6,21.5; FTIR (neat) 3432,1740,1457,1437, 1380, 1311, 1168 cm-1 ; HRMS (M+H) + calculated for C18H35N2O8S 439.2114, found 439.2132.

F Preparation of 2,2'-(2S,2'S)-[(2,3,6,7-Tetrahydro-1, 2,7-thiadiazepine-(4R), (5S)- dihydroxy-1, 1-dioxido-2, 7-diyl) bis-propionic acid dimethyl ester (12a) In a procedure similar to that used for the preparation of diol (12c), the cyclic sulfamide (4a) (100 mg, 0.41 mmol) was subjected to standard osmylation procedures (3 mol% OsO4, NMO, 4: 1 acetone/H2O, 12 hours). Flash chromatography (Si02, Hexanes/EtOAc) afforded 75 mg (53%) of sulfamide (12a) as a clear oil. TLC Rf= 0.09 (1: 2 Hexanes/EtOAc); [α]25D=-5. 52 (c =1. 36, CHCl3) ;'H NMR (CDCl3, 400 MHZ) 4.35 (q, J= 7. 3 Hz, 1H), 4.23 (q, J= 7. 3 Hz, 1H), 3.88 (t, J= 4. 6 Hz, 1H), 3.80-3.72 (m, 2H), 3.77 (s, 3H), 3.75 (s, 3H), 3.66 (dd, J= 15. 6,8.9 Hz, 1H), 3.37 (d, J= 15. 6 Hz, 1H), 3.35 (dd, J= 15. 6,3.1 Hz, 1H), 1.56 (d, J= 7.3 Hz, 3H), 1.53 (d, J= 7.4 Hz, 3H) ; 13C NMR (CDCl3, 100 MHZ) 173.9,173.6,69.4,68.9,58.8,56.5,53.0,52.8, 48.3,45.8,16.3,15.8; FTIR (neat) 3489,3400,2998,1743,1457,1382,1307, 1171 cm-1; HRMS (M+H) + calculated for Cl2H23N208S 355.1175, found 355.1161.

VI. Preparation of 2,2'-(2S,2'S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine- (4R), (5S)- dihydroxy-1, 1-dioxido-2, 7-diyl)]-3,3'-dimethyl bis-butyric acid dimethyl ester (12b).

In a procedure similar to that used for the preparation of diol (12c), the cyclic sulfamide (4b) (66 mg, 0.18 mmol) was subjected to standard osmylation procedures (3 mol% Os04, NMO, 4: 1 acetone/H2O, 12 hours). Flash chromatography (SiO2, Hexanes/EtOAc) afforded 69 mg (96%) of sulfamide (12b) as a clear solid. TLC Rf= 0.33 (1: 2 Hexanes/EtOAc); [a] 25D =-32.7 (c = 0.92, CHC13) ;'H NMR (CDC13, 400 MHZ) 3.94 (d, J= 10.1 Hz, 1H), 3.92-3.83 (m, 2H), 3.86 (d, J= 10. 6 Hz, 1H), 3.76 (s, 3H), 3.74 (s, 3H), 3.59-3.36 (m, 4H), 2. 31-2. 23 (m, 1H), 2.23- 2.14 (m, 1H) 1.04 (d, J= 6.6 Hz, 6H), 0.97 (d, J= 6.4 Hz, 3H), 0.97 (d, J= 6.5 Hz, 3H) ;"C NMR (CDCl3, 100 MHZ) 173.1,172.6,70.0,69.5,67.9,66.0,52.5,52.3,47.4,45.3,28.5,28. 3, 20.0,20.0,19.6,19.5; FTIR (neat) 3457,1740,1436,1380,1311,1158 cm-1 ; HRMS (M+H) + calculated for Cl6H3lN2osS 411. 1801, found 411.1809.

VII. Preparation of 2,2'-(2S,2'S)-[(2,3,6,7-Tetrahydro-1,2,7-thiadiazepine-(4R), (5S)- dihydroxy-1, 1-dioxido-2, 7-diyl) j-3, 3'-diphenyl bis-propionic acid dimethyl ester (12dJ In a procedure similar to that used for the preparation of diol (12c), the cyclic sulfamide (4d) (104 mg, 0.217 mmol) was subjected to standard osmylation procedures (3 mol% OsO4, NMO, 4: 1 acetone/H2O, 12 hours). Flash chromatography (Si02, Hexanes/EtOAc) afforded 110 mg (97.2%) of sulfamide (12d) as a white solid. mp = 153-154°C ; TLC Rf = 0.20 (1: 1 Hexanes/EtOAc) ; [α]25D--131.8 (c=0.22, CHCl3) ;'HNMR (CDC13, 400MHZ) 7.38-7.21 (m, 10H), 4.36 (dd, J= 10. 3,5 Hz, 1H), 4.13 (dd, J= 10. 6,4.1 Hz, 1H), 3.78 (s, 3H), 3.75 (s, 3H), 3.62-3.53 (nfom, 4H), 3.45 (dd, J= 14. 7,5 Hz, 1H), 3.39 (dd, J= 14.4,4.1 Hz, 1H), 3.23 (dd, J=14. 4,10.6 Hz, 1H), 3.12 (dd, J= 14.7,10.4 Hz, 1H), 2.62 (d, J= 12.7 Hz, 1H), 2.52 (dd, J = 16.0,4.6 Hz, 1H) ; 13C NMR (CDC13, 100 MHZ) 173.7,171.0,137.0,136.5,129.5,129.2, 128.6,128.5,127.0,126.9,69.8,68.3,68.0,63.7,53.6,52.6,51.4,4 7.0,36.9,36.6; FTIR (neat) 3502,3382,3029,1741,1716,1604,1559,1497,1312,1153,753,702 cm-1 ; HRMS (M+H) + calculated for C24H3lN208S 507.1801, found 507. 1806.

While Os04, NMO, and Me2CO/H2O were utilized in Parts IV-VII of this procedure, other suitable combinations include: OsO4, NMO, and Me2CO ; and Os04, NMO, and tBuOH/H2O.

EXAMPLE 2 The overall reaction scheme followed in Examples 2A-2B below is set forth in Scheme F.

Scheme F o °i° -\\//15mol% Catalyst1, Ss NU H I T Et3N, OIC to RT reflux, CH2CI2 CH2CII, 56% 1 2 days-- 13 14 69% EXAMPLE 2A In this example, compound (13) was used to prepare N,-Sulfonyl-(3S, 3'5)-bis (4-methyl- l-penten-3-amine) (14). Scheme G depicts these compounds and the reaction in this portion.

Scheme G In a procedure similar to the preparation of the sulfamide (8b), valine-derived allylic amine hydrochloride salt (13) (250 mg, 1.84 mmol) was subjected to sulfamide formation conditions (SO2Cl2, Et3N, CH2C12 0°C-RT, 8 hours). Flash chromatography (SiO2, Hex- anes/EtOAc) yielded 128 mg (56%) of sulfamide (14) as a white solid. mp = 51-52°C ; TLC Rf = 0.48 (3: 1 Hexanes/EtOAc); [a] 2D = 85. 9 (c =0.51, CHCl3); 1H NMR (CDCl3, 400 MHz) 5.70 (ddd, J= 17. 7,10.3,7.8 Hz, 2H), 5.25 (dd, J= 17, 0.9 Hz, 2H), 5.21 (d, J= 10. 2 Hz, 2H), 4.29 (d, J= 7.7 Hz, 2H), 3.65 (dd, J= 7.8,5.4 Hz, 2H), 1.84 (m, 2H), 0.92 (d, J= 6.9 Hz, 6H), 0.89

(d, J= 6.8 Hz, 6H) ; 13C NMR (CDCl3, 100 MHz) 136.5,117.5,62.3,32.4,18.6,17.8; FTIR (neat) 3290,2962,2939,2875,1652,1568,1456,1436,1369,1316,1157 cm-1 ; HRMS (M+H) + calculated for Cl2H25N202S 261.1637, found 261.1646.

In this example, methylene chloride was the solvent utilized. However, acetonitrile, chloroform, toluene, benzene, chlorobenzene, dichlorobenzene, THF, diethyl ether, acetone, DME, and mixtures thereof are also suitable solvents. Furthermore, while Et3N was used as the base, other bases which could be used included pyridine, NaHC03, Na2CO3, K2CO3, NaH, KH, any tertiary amine, and mixtures thereof. Finally, while the procedure was carried out at temperatures of 0-20 ° C, temperatures of anywhere from about-20-20 ° C would also be suitable.

EXAMPLE 2B In this example, N, N-Sulfonyl- (3S, 3'S)-bis (4-methyl-1-penten-3-amine) (14) was used to prepare (3S,6S)-[(2, 3,6,7-Tetrahydro-3,6-bis (methylethyl)-1, 2,7-thiadiazepine-1,1-dioxide (5).

Scheme H depicts these compounds and the reaction in this portion.

Scheme H \/o \\//15mol% catl, Ss H ieflux, CHZQZ l l 1 2days 14 I 69% In a procedure similar to that used for the preparation of cyclic sulfamide (4b), sulfamide (14) (50 mg, 0.192 mmol) was subjected to RCM conditions (reflux CH2C12, 3 mol% catalyst, 15 hours). Flash chromatography (SiO2, Hexanes/EtOAc) afforded 31 mg (69%) of cyclic sulfamide (5) as a white solid. mp =152-153 °C ; TLC Rf = 0. 35 (3: 1 Hex/EtOAc); [α]25D=50. 0 (c = 0. 18, CHCl3) ; 1H NMR (CDCl3, 400 MHz) 5.59 (s, 2H), 4.94 (d, J= 10. 7 Hz, 2H), 3.98 (dd, J=8. 2,4.0 Hz, 2H), 1.92-1.87 (m, 2H), 0.99 (d, J= 6.7 Hz, 6H), 0.92 (d, J= 6.9 Hz, 6H); 13C NMR (CDCl3, 100 MHz) 133.1,56.3,32.6,18.7,17.1; FTIR (neat) 3303,3257,2959,2931,

2875,1653,1559,1321,1161 cm~l ; HRMS (M+H)+ calculated for C10H21N2O2S 233.1324, found 233.1321.

In this example, methylene chloride was the solvent utilized. However, toluene, benzene, chlorobenzene, dichlorobenzene, DME, and mixtures thereof are also suitable solvents.

Furthermore, while Grubbs catalyst I was utilized, Grubbs catalyst 2 and/or 3 could be utilized as well. Finally, while the procedure was carried out at temperatures of 40°C, temperatures of anywhere from about 15-80°C would also be suitable.

EXAMPLE 3 The overall reaction scheme followed in Examples 3A-3H below is set forth in Scheme 1. Each step of this scheme is described in detail in Examples 3A-3H.

Scheme I O O O R 0 0 R2 1. K2co3, CH3COCH3 \\// 'BuO'i N N) C02Me C3H5Br, (94-980/o) s 1-1 H H 2. RCM, Catalyst 1 (840/o) 2 _ 3. TFA (83%) 15b : R2 = CH (CH3) 2 3. TFA (83%) 4. BnBr NaH (96%) 16d 15d:R=CH2Ph 1. PPh3, DEAD (-)-Ethyl Lactate (81%) 2. TFA, (99%) 0 0 R2 O O RZ 1. KZC03, CH3CN X/Ss | C3HsBr, (81%) EtO2C N N CO2Me EtO2C N N Corme H H 2. RCM, Catalyst 1, (99%) 6b 17b

EXAMPLE 3A In this example, compound (15d) was used to prepare N, N-bis (2-propenyl)-N-(tert- butoxycarbonylsulfonyl) L-phenylalanine methyl ester. Scheme J depicts these compounds and the reaction in this portion.

Scheme J Anhydrous K2CO3 (0.771 g, 5.58 mmol) and allyl bromide (0.507 mL, 0.709 g, 5.86 mmol) were added to a stirring solution of (15d) (0.500 g, 1. 40 mmol) in dry acetone (14 mL) in an oven-dried 50 mL round-bottom flask. The mixture was heated to reflux under argon for 12 hours. The solution was then cooled, suction filtered, and the solvent removed under reduced pressure to give a pale yellow oil. Flash chromatography (SiO2, 4: 1 Hexane: EtOAc) yielded 504 mg (82%) of a clear, colorless oil. TLC Rf = 0.35 (4: 1 Hexanes/EtOAc); [α]25D =-27.2 (c = 1.00, CHCl3);1H NMR (CDCl3, 400 MHz) 8 7.31-7.21 (m, 5H), 5.98-5.82 (m, 2H), 5.28 (ddd J=15. 8,3.6,1.5 Hz, 2H), 5.17 (ddd, J= 10.3,5.1, 1.3 Hz, 2H), 4.83 (dd, J= 9.0,6.2 Hz, 1H), 5.28 (ddd 15. 8,3.5,1.5 Hz, 2H), 5.17 (ddd J= 9. 2,5.1,1.3 Hz, 2H), 4.32 (dd J=9. 0,6.2 Hz, 1H), 4.22 (m, 3H), 4.12 (dd, J=16. 6,6.8 Hz, 1H), 3.62 (s, 3H), 3.26 (dd J=13. 6,9.0 Hz, 1H), 3.05 (ddJ= 13.6,6.2 Hz, 1H), 1.51 (s, 9H) ; 13C NMR (CDCL, 100 MHz) 171.3,151.6,136.9,135.5,133.6,129.7,128.9, 127.3,118.0,117.9,84.0,62.1,52.4,51.6,50.0,37.5,28.5; FTIR (neat) 3082,2980,1731, 1644,1605,1494,1442,1371,1319,1249,1149,1036 cm-' ; HRMS (M+H)+ calculated for C2lH30N206S 439.1903, found 439.1882.

In this example, acetone was the solvent utilized. However, acetonitrile, chloroform, toluene, benzene, chlorobenzene, dichlorobenzene, THF, diethyl ether, methylene chloride,

DME, and mixtures thereof are also suitable solvents. Furthermore, while K2CO3 was used as the base, other bases which could be used include pyridine, NaHC03, Na2CO3, NaH, KH, and mixtures thereof. The allylating agent utilized was allyl bromide, but the following would work as well: allyl iodide; allyl bromide, NaI ; allyl bromide, KI; and allyl bromide, Bu4NI. Finally, while the procedure was carried out at temperatures of 56°C or 82°C, temperatures of anywhere from about 40-100°C would also be suitable.

EXAMPLE 3B In this example, N, N-bis (2-propenyl)-N (tert-butoxycarbonylsulfonyl) L-phenylalan- ine methyl ester was used to prepare 2-(2S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1- dioxido-7-tert-butyloxycarbonyl-2-yl)] 3-phenyl-propionic acid methyl ester. Scheme K depicts these compounds and the reaction in this portion.

Scheme K O O O S tBuo N N CO2Me 2 RCM Catalyst 1 (84%) tBuo) 4No oN CO Me ,, z In a procedure similar to that used for the preparation of cyclic sulfamides (4b), the bisallylated- (15d) (100 mg, 0.228 mmol) was subjected to RCM reaction and work-up conditions (reflux CH2C12, 3 mol% Catalyst 1,1.5 hours). Flash chromatography (SiO2, 4: 1 Hexanes/EtOAc) yielded 78 mg (84%) of cyclic sulfamide as a clear, colorless oil. TLC Rf = 0.22 (4: 1 Hexanes/EtOAc); [a] 25D =-37. 7 (c = 1. 00, CHC13) ;'H NMR (CDC13, 400 MHz) 8 7.32-7.24 (m, 5H), 5.81 (ddd J=11. 2,5.5,5.5 Hz, 1H), 5.67 (dddJ=11. 3,3.7,3.7 Hz, 1H), 5.09 (t, J = 7.8 Hz, 1H), 4.15 (dd J=16.9,5.7 Hz, 2H), 4.08 (m, 2H), 3.86 (ddd J =16.9,5.1,1.3 Hz, 1H), 3.68 (s, 3H), 3.29 (dd J=14. 0,7.6 Hz, 1H), 2.99 (dd J=14. 0,7.9 Hz, 1H), 1.50 (s, 9H); 13C NMR (CDCl3, 100 MHz) 8 170.4,151.3,135.7,129.2,128.6, 128.3,127.3,127.1,83.8,61.6,52.3,43.8,43.5,37.0,27.8; FTIR (neat) 3031,2981,2259,

1732,1604,1496,1445,1367,1327,1265,1151,1093,1037,918 cni-' ; HRMS (M+H) + calculated for Cl9H26N206S 411.1590 found 411.1582.

In this example, methylene chloride was the solvent utilized. However, toluene, benzene, chlorobenzene, dichlorobenzene, DME, and mixtures thereof are also suitable solvents. Furthermore, while Grubbs catalyst 1 was utilized, Grubbs catalyst 2 and/or 3 could be utilized as well. Finally, while the procedure was carried out at temperatures of 40°C, temperatures of anywhere from about 15-80°C would also be suitable.

EXAMPLE 3C In this example, the 2-(2S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-7- tert-butyloxycarbonyl-2-yl)] 3-phenyl-propionic acid methyl ester prepared in Example 3B was used to prepare 2-(2S)-[(2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-2-yl)] 3- phenyl-propionic acid methyl ester. Scheme L depicts these compounds and the reaction in this portion.

Scheme L The Boc-protected cyclic sulfamide prepared in Example 3B (50 mg, 0.122 mmol) was added to an oven-dried 5 mL round-bottom flask, followed by trifluoroacetic acid (TFA) (194 mg, 0.131 mL, 1.71 mmol). The solution was stirred at ambient temperature for 36 hours. CH2C12 (4 mL) was added and the resulting solution washed with saturated NaHC03 (3 times). The aqueous solution was back-extracted with methylene chloride (2 times), and the combined organic solutions washed with water, brine, and dried Na2SO4.

The solution was suction filtered, and the solvent removed under reduced pressure. Flash chromatography (SiO2, 2: 1 Hexanes/EtOAc) yielded 31.2 mg (83%) of a white crystalline solid. mp = 62-64°C ; TLC Rf= 0.20 (2: 1 Hexanes/EtOAc); [a] =-59.85 (c = 1.00,

CHCl3) ; 1HNMR (CDC13, 400 MHz) 8 7.31-7.21 (m, 5H), 5.73-5.65 (m, 1H), 5.64-5.57 (m, 1H), 4.97 (dd J= 8.0,5.7 Hz, 1H), 4.86 (dd J= 8.9,6.8 Hz, 1H), 4.04 (ddd J= 8.9,2. 3,1.7 Hz, 1H), 3.81 (ddd J=18. 5,5.1,1.0 Hz, 1H), 3.69 (s, 3H), 3.47 (dddJ=18. 0,4.6,1.0 Hz, 1H), 3.44-3.34 (m, 1H), 3.29 (ddJ= 26. 1,6.8 Hz, 1H), 2.96 (ddJ=14. 3,8.9 Hz, 1H) ; 13C NMR (CDC13, 100 MHz) 8 171.0,136.2,129.7,129.5,129.2,128.4,128.2,128.0,126.9, 126.7,61.0,52.2,41.9,40.7,36.3; FTIR (neat) 3297,3030,2952,2861,1737,1603,1496, 1437,1348,1318,1238,1161,1095,983,917,887 cm-1 ; HRMS (M+H)+ calculated for C14H18N2O4S 311.1066 found 311.1060.

Other suitable solvents which could be utilized in this preparation procedure include: methylene chloride, THF, ether, acetonitrile, benzene, and mixtures thereof. Furthermore, while TFA was utilized as the acid, NaHSO4 and HC1 could be utilized as well. Finally, while the procedure was carried out at room temperature, temperatures of anywhere from about 0-80°C would also be suitable.

EXAMPLE 3D In this example, 2- (2S)- [ (2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-2-yl)] 3-phenyl-propionic acid methyl ester prepared in Example 3C was used to prepare 2- (2S)- [ (2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-7-benzyl-2- yl)] 3-phenyl-propionic acid methyl ester (16). Scheme M depicts these compounds and the reaction in this portion.

Scheme M Anhydrous K2CO3 (44 mg, 0.318 mmol) and benzyl bromide (40 RL, 0.057g, 0.334 mmol) were added to a stirring solution of the cyclic sulfamide prepared in Example 3C (49 mg, 0.159 mmol) in dry CH3CN (1.5 ml), in an oven dried 5 mL round-bottom flask. The

solution was heated to 55°C and stirred for 36 hours, cooled to room temperature, and diluted with CH2Cl2 (3 mL). The solution was filtered, and the solvent removed under reduced pressure. Flash chromatography (SiO2, 4: 1 Hexanes/EtOAc) yielded 62 mg (96%) of sulfamide (16) as a white crystalline solid, mp = 56-58°C ; TLC Rf = 0.26 (4: 1 Hexanes/EtOAc) ; [a] 25D =-36.8 (c=1.00, CHC13) ;'H NMR (CDC13, 400 MHz) 8 7.33-7.20 (m, 10H), 5.83 (dddJ=10. 9,4.4,1.3 Hz, 1H), 5.45 (dddJ= 9.8,5.3,4.4 Hz, 1H), 4.97 (dd J= 9.3,6.4 Hz, 1H), 4.03 (s, 2H), 3.98 (ddd J= 18.2,4.0,2.0 Hz, 1H), 3.76 (ddJ= 18.2, 5.8 Hz, 1H), 3.73 (s, 3H), 3.53 (dd J= 17.8,3.9 Hz, 1H), 3.38 (d, 2.4 Hz, 1H), 3.32 (dd J = 14. 3,6.4 Hz, 1H), 2.95 (ddJ= 18.2,8.9 Hz, 1H); 13C NMR (CDCl3, 100 MHz) 8 171.2, 136.4,136.1,129.5,128.8,128.5,128.4,128.1,127.7,127.3,126.9, 61.4,52.3,51.1,43.5, 41.6,36.6; FTIR (neat) 3065,3026,2951,1740,1603,1498,1451,1439,1354,1320,1160, 1099,926,753 cm-1 ; HRMS (M+H) +calculatedforC2lH24N204S401. 1535 found 401. 1556.

In this example, THF was the solvent utilized. However, methylene chloride, DME, Et2O, DMF, and mixtures thereof are also suitable solvents. Furthermore, while BnBr and NaH were used as the benzylating agent and base, respectively, other suitable combinations include the following: BnBr with Nal, KH, and/or KHMDS ; BnCI with NaI, KH, and/or KHMDS ; and CC13CN and KH with BnOH and TfOH.

EXAMPLE 3E In this example, compound (15b) was used to prepare N-tert-butyloxycarbonyl-N'- [[(1R)-1-ethoxycarbonyl-ethylamino]sulfonyl]-L-valine methyl ester. Scheme N depicts these compounds and the reaction in this portion.

Scheme N 0 0 0 R' 1. PPh3, DEAD \/ A JL I. , DEAD 00 Y 'BuON N COZMe -)-Ethyl L, actate (81%) " J" H H tBuO O 15b : R = CH (CH3) 2

Diethyl azodicarboxylate (DEAD) (508 1L, 3.23 mmol) was added via dropwise addition to a stirring solution of the sulfamide (15b) (1.00 g, 3.23 mmol) in THF (2 mL).

A solution consisting of (L)- (-)-Ethyl lactate (366 jj. L, 3.23 mmol) and PPh3 (847 mg, 3.23 mmol) in THF (3 mL), was slowly cannulated into the sulfamide solution. After 3 hours, the reaction mixture was concentrated and dissolved in ether to precipitate Ph3PO. The solution was filtered, and concentrated under reduced pressure to leave a crude oil. Flash chromatography (SiO2, 5: 1 Hexanes/EtOAc) yielded 1. 07 g (81%) of Boc-protected sulfamide as a yellow oil. TLC Rf= 0.63 (2: 1 Hexanes/EtOAc); [a] 25D = +68.8 (c = 1.00, CHC13) ;'H NMR (400 MHz, CDCl3) 8 6.13 (d, J=7. 6 Hz, 1H), 4.89 (q, J= 6.9 Hz, 1H), 4.19 (q, J= 7.1 Hz, 2H), 4.09 (dd, J= 7. 6,4.0 Hz, 1H), 3.77 (s, 3H), 2.18-2.15 (m, 1H), 1.57 (d, J= 6. 9 Hz, 3H), 1.50 (s, 9 H), 1.27 (t, J= 7.1 Hz, 3H), 1.01 (d, J= 6.8 Hz, 3H), 0.89 (d, J= 6.9 Hz, 3H) ; 13C NMR (100 MHz, CDC13) S 71.15,170.10,150.34,128.22,84.82, 61.52,61.48,56.11,52.29,32.01,27.73,18.62,17.14,16.45,14.03; FTIR (neat) 3297, 1742, 1458,1429, 1371,1299,1277, 1262, 1219, 1153 cm-1; HRMS (M+H)+ calculated for C16H31N2O8S 411. 1801, found 411. 1784.

In this example, THF was the solvent utilized. However, methylene chloride, Et2O, DMF, CH3CN, benzene, and mixtures thereof are also suitable solvents. Furthermore, while Ph3P and DEAD were used as the reagents, other suitable reagents include: polymer-bound Ph3P ; Ph3As ; diisopropyl azodicarboxylate (DIAD); ditertbutyl azocarboxylate (DTEB); and dicyclohexyl azodicarboxylate (DCAD). Finally, while the procedure was carried out at room temperature, temperatures anywhere from 0 ° C to room temperature would also work.

EXAMPLE 3F In this example, the N-tert-butyloxyvarbonyl-N'-[[(lR)-l-ethoxycarbonyl- ethylamino3sulfonyl]-L-valine methyl ester prepared in Example 3E was used to prepare N- [[(lR)-l-ethoxycarbonyl-ethylamino] sulfonyl]-L-valine methyl ester (17b). Scheme O depicts these compounds and the reaction in this portion.

Scheme O

A stirring solution ofthe Boc-protected sulfamide prepared in Example 3E (500 mg, 1.22 mmol) in CH2Cl2 (1.0 mL) was prepared and TFA (1.41 mL, 18.3 mmol) was added to this solution. After 1.5 hours, the reaction mixture was diluted with CH2Cl2 (15 mL) and washed with NaHC03 (2 times), H2O (2 times), brine (2 times), and dried (Na2SO4). The organic layer was concentrated under reduced pressure. Flash chromatography (Si02, 1: 1 Hexanes/EtOAc) yielded 380 mg (100%) of sulfamide (17) as a light yellow solid. mp = 79-81°C ; TLC Rf = 0. 55 (1: 1 Hexanes/EtOAc); [α]25D=-129. 2 (c=0. 5, CDCL) ;'H NMR (400 MHZ, CDC13) 6 4.98 (t, J=10. 1,8.9 Hz, 2H), 4.22 (q, J= 7.1 Hz, 2H), 4.01 (m, 1H), 3.80 (dd, J= 10.2,4.9 Hz, 1H), 3.77 (s, 3H), 2.08 (m, 1H), 1.37 (d, J= 7.1 Hz, 3H), 1.26 (t, J= 7.1 Hz, 3H), 0.98 (d, J= 6.8 Hz, 3H), 0.88 (d, J= 6.9Hz, 3H) ; 13C NMR (100 MHZ, CDC13) 8 173.01,172.87,61.73,61.09,52.36,51.66,31.16,19.20,18.88,17.4 5,14.02; FTIR (neat) 3282,1742,1451,1437,1349,1299,1270,1139 cm-1; HRMS (M+H) + calculated for C"H23N206S 311.1277, found 311.1262.

Other suitable solvents which could be utilized in this preparation procedure include: methylene chloride, THF, Et2O, CH3CN, and mixtures thereof. Furthermore, while TFA was utilized as the acid, NaHSO4 and HC1 could be utilized as well. Finally, while the procedure was carried out at room temperature, temperatures of anywhere from about 0- 80°C would also be suitable.

EXAMPLE 3G In this example, N-[[(lR)-l-ethoxycarbonyl-ethylamino] sulfonyl]-L-valine methyl ester (17) was used to prepare N, N'-bis (2-propenyl)-N-[[(lR)-l-ethoxycarbonyl- ethylamino] sulfonyl]-L-valine methyl ester. Scheme P depicts these compounds and the reaction in this portion.

Scheme P

A stirring solution of sulfamide (17) (150 mg, 0.484 mmol) in CH3CN (2.5 mL) in a 10 mL flame dried round-bottom flask was prepared and allyl bromide (0.105,1.21 mmol) followed by K2CO3 (167 mg, 1.21 mmol) was added to this solution. The reaction was allowed to stir overnight at room temperature. The reaction mixture was washed with H20 (2 times), brine (2 times), and dried (Na2SO4). The organic layer was concentrated under reduced pressure. Flash chromatography (Si02, 1: 1 Hexanes/EtOAc) yielded 152 mg (81 %) of the bis-allylated sulfamide as clear oil. TLC Rf= 0.56 (2: 1 Hexanes/EtOAc); [a] 25D =- 30.2 (c= 1. 00, CHC13) ;'HNMR (400MHZ, CDC13) 6 5.93 (m, 2H), 5.21 (m, 2H), 5. 15 (dd, J= 10.2,1.2 Hz, 1H), 5.11 (dd, J= 10.1,1.1 Hz, 1H), 4.19 (m, 3H), 4.07 (d, J= 10.6 Hz, IH), 3.99 (m2H), 3.90 (dd, J= 16. 2,6.16 Hz, 1H), 3.78 (dd, 16.1,6.6 Hz, 1H), 3.70 (s, 3H), 2.20 (m, 1H), 1.48 (d, J= 7.2 Hz, 3H), 1.28 (t, J= 7.1 Hz, 3H), 1.02 (d, J= 6.6 Hz, 3H), 0.92 (d, J= 6. 5 Hz, 3H) ; 13C NMR (100 MHZ, CDCl3) d 171.62,171.52,135.12,134.67, 118. 08,117.60,66.25,61.29,55.49,51.69,49.02,47.84,28.23,19.62,19 .56,15.45,14.05 ppm; FTIR (neat) 2982,2890,1741, 1643, 1441, 1380, 1336, 1297, 1336,1297,1205, 1160, 1134 cm~', HRMS (M+H) + calculated for C17H31N2O6S 391.1903, found 391.1879.

In this example, acetone was the solvent utilized. However, acetonitrile, chloroform, toluene, benzene, chlorobenzene, dichlorobenzene, THF, diethyl ether, methylene chloride, DME, and mixtures thereof are also suitable solvents. Furthermore, while K2CO3 was used as the base, other bases which could be used include pyridine, NaHCO3, Na2CO3, NaH, KH, and mixtures thereof. The allylating agent utilized was allyl bromide, but the following would work as well: allyl iodide; allyl bromide, NaI ; allyl bromide, KI; and allyl bromide, Bu4NI. Finally, while the procedure was carried out at temperatures of 56°C or 82°C, temperatures of anywhere from about 40-100°C would also be suitable.

EXAMPLE 3H In this example, N, N'-bis (2-propenyl)-N [ [ (lR)-l-ethoxycarbonyl- ethylamino] sulfonyl]-L-valine methyl ester was used to prepare 2-(2S)-[(7-[(1'R)-1'= ethoxycarbonyl-ethyl]-2, 3,6,7-Tetrahydro-1,2,7-thiadiazepine-1,1-dioxido-2-yl)] 3-phenyl propionic acid methyl ester (6). Scheme Q depicts these compounds and the reaction in this portion.

Scheme Q OO O O 0 0 02C (Me 2. RCM, Catalyst 1 (99%) 1 z S s sX 0'Et N N CO2Me zu To a stirring solution of the bis-allylated sulfamide prepared in the previous experimental (60 mg, 0. 153 mmol) in degassed CH2Cl2in a 10 mL flame dried round-bottom flask is taken up in 3.1 mL (0.05 M). The Grubbs metathesis catalyst (6.32 mg, 0.008 mmol) was then added. After 10 minutes, the solution color changed from purple to brown.

After 3 hours, TLC showed the reaction was complete. The reaction solution was then concentrated down and directly purified by column chromatography to yield 31. 6 mg (67%) of the cyclic sulfamide (6) as pure brown oil. TLC Rf = 0.37 (2: 1 Hexanes/EtOAc); [a] 25D =-24. 0 (c = 0.25, CDC13) ;'H NMR (400 MHZ, CDC13) 6 5.58 (m, 2H), 4.71 (q, J= 7.3, 1H), 4.18 (q, J=7. 1 Hz, 2H), 4.11 (m, 3H), 3.72 (dd J=18. 8,4.9 Hz, 1H), 3.66 (s, 3H), 3.61 (dd, J= 18.3,4.5 Hz, 1H), 2.14 (m, 1H), 1.39 (d, J= 7.3 Hz, 3H), 1.28 (t, J= 7.2 Hz, 3H), 1.01 (d, J= 6.7 Hz, 3H), 0.91 (d, J= 6.5 Hz, 3H) ; 13C NMR (100 MHZ, CDC13) d 171.52,170.87,127.51,127.21,64.87,61.43,55.65,51.80,41.67,41 .02,27.47,19.13, 18.90,16.29,14.10; FTIR (neat) 1742,1589,1458,1437,1361,1357,1320,1190, 1161 cm- 1 ; HRMS (M+H)+ calculated for C15H27N2O6S 363.1590, found 363.1575.

In this example, methylene chloride was the solvent utilized. However, toluene, benzene, chlorobenzene, dichlorobenzene, DME, and mixtures thereof are also suitable

solvents. Furthermore, while Grubbs catalyst 1 was utilized, Grubbs catalyst 2 and/or 3 could be utilized as well. Finally, while the procedure was carried out at temperatures of 40 ° C, temperatures of anywhere from about 15-80 ° C would also be suitable.

EXAMPLE 4 The inhibition of Herpes Simplex Virus Protease bythe compound shown in Scheme R was determined according to the procedure described by Waxman et al., Antiviral Chemistry and Chemotherapy, 11: 1-22 (1999); Qiu et al., Proteases of Infectious Agents, Academic Press, 93-115 (1999); and U. S. Patent NO. 6,008,033, each incorporated by reference herein.

The compound was tested at a concentration of 200 molar.

Scheme R The compound resulted in a 20.5% inhibition of the protease.

EXAMPLE 5 The inhibition of HIV protease by the compounds shown in Scheme S (99 llmolar), Scheme T (99 polar), and Scheme U (99 , molar) was determined following the procedure described by Maschera et al., Human Immunodeficiency Virus: Mutations in the Viral Protease that Confer Resistance to Saquinavir Increase the Dissociation Rate Constant for the Protease-Saquinavir Complex, J. Biol. Chem., 271: 33231-35 (1996), incorporated by reference herein.

Scheme S

Scheme T Scheme U

The compound of Scheme S resulted in a 96% inhibition of the protease, while the compound of Scheme T resulted in a 22% inhibition. Finally, the compound of Scheme U resulted in a 21% inhibition.

EXAMPLE 6 The inhibition of human cathepsin K by the compounds shown in Schemes V (106 polar) and W (106 umolar) was determined.

Scheme V Scheme W The peptides utilized were chromophoric Z-Phe-Arg-pNA*HCl and fluorogenic Z- Phe-Arg-AMC (each available from BACHEM Bioscience, Inc.). Stock solutions were prepared with 100% dimethylsulfoxide (DMSO) and stored at-20°C.

The final assay conditions were 100 mM NaOAc, pH of 5.5,10 mM (R, R)-dithiothreitol (DTT), 10% DMSO, 120mMNaCl, 10 umolarofthepeptide, and 12.5- 0.012 molar inhibitor in twofold serial dilutions from 20X stocks in 100% DMSO. The enzyme working solution was a 1: 3000 dilution of 8.75 mM human cathepsin K into 100 mM of NaOAc, pH of 5.5,10 mM DTT, 1.2 M NaCl.

Serial dilutions were performed in 100% DMSO. Columns 2-12 of an intermediate plate were filled with 100 mL/well of 100% DMSO and column 1 was filled with 195 mL/well. Next, 5 mL of a 10 mM solution was added to column 1. A twofold serial dilution was prepared in columns 1-11 ofthe intermediate plate by sequential transfer of 100 mL of the contents of each well in a column to the corresponding well of the next column, with mixing between each transfer. Samples of 11.7 mL from each well of the intermediate plate were transferred to another intermediate plate and 200 mL of apeptide buffer (105 mM NaOAc, pH of 5.5,10.5 mM DTT, 5.8% DMSO, 12 mM peptide) was added. Samples (20 pL) of enzyme working solution were placed in each well of an empty assay plate, and 180 pL/well of the test compound-peptide mixture was added to the enzyme to initial the assay.

The fluorescence was monitored every 6 minutes for 72 minutes using a CytoFluor Series 4000 PerSeptive Biosystems multi-well fluorescence plate reader with the following settings: gain=50 ; 20 reads/well; 32°C ; lex=36020 nm; and lem=440i20 nm.

The compound of Scheme V resulted in a 20% inhibition of human cathepsin K, while the compound of Scheme W resulted in a 14% inhibition of human cathepsin K.