Title:
AMINOACYL-TRNA SYNTHASE MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/040885
Kind Code:
A1
Abstract:
The present invention relates to an aminoacyl-tRNA synthase mutant and use thereof. The aminoacyl-tRNA synthase mutant is obtained by means of mutation of an amino acid sequence represented by SEQ ID NO: 19, the mutation at least comprising the following mutation site: I at position 159 is mutated into a hydroxyl-containing amino acid, a basic amino acid or a non-polar amino acid. By means of a method of site-directed mutation, the amino acid sequence is changed, realizing the alteration of the protein structure and function; the mutant has relatively high activity and specificity, so that the mutant can be used for realizing efficient and site-directed introduction of non-natural amino acids in proteins.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
ZHAO JUNQI (CN)
WANG SHUYU (CN)
MENG XIANGYU (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
ZHAO JUNQI (CN)
WANG SHUYU (CN)
MENG XIANGYU (CN)
Application Number:
PCT/CN2023/076493
Publication Date:
February 29, 2024
Filing Date:
February 16, 2023
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/06; C12N15/53; C12P13/04
Domestic Patent References:
| WO2022156042A1 | 2022-07-28 |
Foreign References:
| CN106047846A | 2016-10-26 | |||
| CN103820410A | 2014-05-28 | |||
| CN104004723A | 2014-08-27 | |||
| CN104059891A | 2014-09-24 | |||
| US20090226963A1 | 2009-09-10 |
Other References:
ROBERTSON WESLEY E., FUNKE LOUISE F. H., DE LA TORRE DANIEL, FREDENS JULIUS, ELLIOTT THOMAS S., SPINCK MARTIN, CHRISTOVA YONKA, CE: "Sense codon reassignment enables viral resistance and encoded polymer synthesis", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 372, no. 6546, 4 June 2021 (2021-06-04), US , pages 1057 - 1062, XP093137517, ISSN: 0036-8075, DOI: 10.1126/science.abg3029
CHIN J W, ET AL.: "Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 99, no. 17, 20 August 2002 (2002-08-20), pages 11020 - 11024, XP003006832, ISSN: 0027-8424, DOI: 10.1073/pnas.172226299
"Biology of Aminoacyl-tRNA Synthetases", vol. 48, ELSEVIER, ISBN: 978-0-12-820260-9, ISSN: 1874-6047, article KRAHN NATALIE, THARP JEFFERY M., CRNKOVIĆ ANA, SÖLL DIETER: "Engineering aminoacyl-tRNA synthetases for use in synthetic biology", pages: 351 - 395, XP093048412, DOI: 10.1016/bs.enz.2020.06.004
CHIN J W, ET AL.: "Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 99, no. 17, 20 August 2002 (2002-08-20), pages 11020 - 11024, XP003006832, ISSN: 0027-8424, DOI: 10.1073/pnas.172226299
"Biology of Aminoacyl-tRNA Synthetases", vol. 48, ELSEVIER, ISBN: 978-0-12-820260-9, ISSN: 1874-6047, article KRAHN NATALIE, THARP JEFFERY M., CRNKOVIĆ ANA, SÖLL DIETER: "Engineering aminoacyl-tRNA synthetases for use in synthetic biology", pages: 351 - 395, XP093048412, DOI: 10.1016/bs.enz.2020.06.004
Attorney, Agent or Firm:
KANGXIN PARTNERS, P. C. (CN)
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