Title:
AMYOTROPHIC LATERAL SCLEROSIS ANIMAL MODEL USING TRANSGENIC ZEBRAFISH AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/229363
Kind Code:
A1
Abstract:
The present invention relates to a transgenic zebrafish animal model for amyotrophic lateral sclerosis that is induced to overexpress the hFUS(R521C) gene, a construction method therefor, and a method for screening a drug for amyotrophic lateral sclerosis, using same. The animal model construction method of the present invention utilizes a binary expression system, thereby allowing for the stable production of uniform animal models at a large scale. The disease is rapidly induced in the animal models constructed by the method of the present invention and thus the animal models can be advantageously used in amyotrophic lateral sclerosis research including drug screening, etc.
Inventors:
PARK HAE CHUL (KR)
LEE JEONG HYEON (KR)
SEO YONG-BO (KR)
LEE JEONG SOO (KR)
RO HYUN JU (KR)
LEE JEONG HYEON (KR)
SEO YONG-BO (KR)
LEE JEONG SOO (KR)
RO HYUN JU (KR)
Application Number:
PCT/KR2023/007053
Publication Date:
November 30, 2023
Filing Date:
May 24, 2023
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
IAC IN NAT UNIV CHUNGNAM (KR)
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
IAC IN NAT UNIV CHUNGNAM (KR)
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
A01K67/027; C12N15/85; C12Q1/6897
Domestic Patent References:
| WO2000049150A1 | 2000-08-24 |
Foreign References:
| KR20020016986A | 2002-03-07 |
Other References:
ACOSTA JAMIE RAE, GOLDSBURY CLAIRE, WINNICK CLAIRE, BADROCK ANDREW P., FRASER STUART T., LAIRD ANGELA S., HALL THOMAS E., DON EMIL: "Mutant Human FUS Is Ubiquitously Mislocalized and Generates Persistent Stress Granules in Primary Cultured Transgenic Zebrafish Cells", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 9, no. 6, US , pages e90572, XP093111304, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0090572
CHIO ADRIANO; MAZZINI LETIZIA; MORA GABRIELE: "Disease-modifying therapies in amyotrophic lateral sclerosis", NEUROPHARMACOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 167, 3 February 2020 (2020-02-03), AMSTERDAM, NL, XP086088810, ISSN: 0028-3908, DOI: 10.1016/j.neuropharm.2020.107986
HONG JEONGKWAN, LEE JAE-GEUN, SOHN KYUNG-CHEOL, LEE KAYOUNG, LEE SEOEE, LEE JINYOUNG, HONG JIHYE, CHOI DONGJU, HONG YESEUL, JIN HY: "IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish", COMMUNICATIONS BIOLOGY, vol. 4, no. 1, XP093111312, ISSN: 2399-3642, DOI: 10.1038/s42003-021-02923-3
CHIO ADRIANO; MAZZINI LETIZIA; MORA GABRIELE: "Disease-modifying therapies in amyotrophic lateral sclerosis", NEUROPHARMACOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 167, 3 February 2020 (2020-02-03), AMSTERDAM, NL, XP086088810, ISSN: 0028-3908, DOI: 10.1016/j.neuropharm.2020.107986
HONG JEONGKWAN, LEE JAE-GEUN, SOHN KYUNG-CHEOL, LEE KAYOUNG, LEE SEOEE, LEE JINYOUNG, HONG JIHYE, CHOI DONGJU, HONG YESEUL, JIN HY: "IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish", COMMUNICATIONS BIOLOGY, vol. 4, no. 1, XP093111312, ISSN: 2399-3642, DOI: 10.1038/s42003-021-02923-3
Attorney, Agent or Firm:
MUHANN PATENT & LAW FIRM (KR)
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