Title:
ANCESTRAL SEQUENCE RECONSTRUCTION-BASED ω-TRANSAMINASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2023/103947
Kind Code:
A1
Abstract:
Provided is an ancestral sequence reconstruction-based ω-transaminase mutant from Aspergillus terreus. The amino acid sequence of the mutant is as shown in SEQ ID NO: 4 or SEQ ID NO: 6. Compared with a wild-type enzyme, the thermal stability of the ω-transaminase mutant is improved. Specifically, the half-life of the mutant is 24 h or above, and the half-life of the wild-type enzyme is 6.90 min. The semi-inactivation temperature of the mutant is about 49.00°C, and the semi-inactivation temperature of the wild-type enzyme is 37.89°C.
Inventors:
HUANG JUN (CN)
CAI TINGTING (CN)
QIU SHUAI (CN)
MEI LEHE (CN)
CAO JIAREN (CN)
LYU CHANGJIANG (CN)
FAN FANGFANG (CN)
HU SHENG (CN)
LI YE (CN)
ZHAO WEIRUI (CN)
WANG FENG (CN)
LI YUANYUAN (CN)
CAI TINGTING (CN)
QIU SHUAI (CN)
MEI LEHE (CN)
CAO JIAREN (CN)
LYU CHANGJIANG (CN)
FAN FANGFANG (CN)
HU SHENG (CN)
LI YE (CN)
ZHAO WEIRUI (CN)
WANG FENG (CN)
LI YUANYUAN (CN)
Application Number:
PCT/CN2022/136528
Publication Date:
June 15, 2023
Filing Date:
December 05, 2022
Export Citation:
Assignee:
ZHEJIANG UNIV OF SCIENCE & TECHNOLOGY (CN)
International Classes:
C12N9/10; C12N15/54; C12P7/26
Foreign References:
| CN114134128A | 2022-03-04 | |||
| CN112481230A | 2021-03-12 | |||
| CN108913671A | 2018-11-30 | |||
| CN110144335A | 2019-08-20 | |||
| CN112359030A | 2021-02-12 | |||
| CN104480155A | 2015-04-01 |
Other References:
KUN ZHANG; YIFEI DAI; JINDI SUN; JIACHEN LU; KEQUAN CHEN: "Enzyme ancestral sequence reconstruction and directed evolution", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 37, no. 12, 25 December 2021 (2021-12-25), CN , pages 4187 - 4200, XP009546658, ISSN: 1000-3061, DOI: 10.13345/j.cjb.200791
WILDING MATTHEW, PEAT THOMAS S., KALYAANAMOORTHY SUBHA, NEWMAN JANET, SCOTT COLIN, JERMIIN LARS S.: "Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction", GREEN CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 19, no. 22, 1 January 2017 (2017-01-01), GB , pages 5375 - 5380, XP093069150, ISSN: 1463-9262, DOI: 10.1039/C7GC02343J
WILDING MATTHEW, PEAT THOMAS S., KALYAANAMOORTHY SUBHA, NEWMAN JANET, SCOTT COLIN, JERMIIN LARS S.: "Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction", GREEN CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 19, no. 22, 1 January 2017 (2017-01-01), GB , pages 5375 - 5380, XP093069150, ISSN: 1463-9262, DOI: 10.1039/C7GC02343J
Attorney, Agent or Firm:
HANGZHOU TIANQIN INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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