Title:
ANTI-BSA RABBIT MONOCLONAL ANTIBODY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/093042
Kind Code:
A1
Abstract:
Provided are an anti-BSA rabbit monoclonal antibody and use thereof, which belong to the technical field of antibodies. The anti-BSA rabbit monoclonal antibody or an antigen-binding portion thereof specifically binds to BSA. The monoclonal antibody or the antigen-binding portion thereof comprises a heavy-chain variable region VH and a light-chain variable region VL. The heavy-chain variable region comprises heavy-chain CDR-H1, heavy-chain CDR-H2 and heavy-chain CDR-H3. The light-chain variable region comprises light-chain CDR-L1, light-chain CDR-L2 and light-chain CDR-L3. The antibody can be used for detecting bovine serum albumin (BSA) in foods such as milk, and detecting and removing a trace amount of residual BSA in a cell culture supernatant, a tissue engineered medical product and a vaccine. The rabbit monoclonal antibody targeting the BSA is acquired by means of a phage display technology. The antibody can specifically recognize the BSA with high affinity.
Inventors:
DING HUI (CN)
WANG YUFANG (CN)
DU JIWEN (CN)
YANG YAN (CN)
HUANG RONGRONG (CN)
WANG YUFANG (CN)
DU JIWEN (CN)
YANG YAN (CN)
HUANG RONGRONG (CN)
Application Number:
PCT/CN2023/075810
Publication Date:
May 10, 2024
Filing Date:
February 14, 2023
Export Citation:
Assignee:
BIOINTRON BIOLOGICAL INC (CN)
International Classes:
C07K16/18; C07K14/76; C12N15/13; C12N15/79; G01N33/543; G01N33/68
Foreign References:
| EP0131415A2 | 1985-01-16 | |||
| CN114591430A | 2022-06-07 | |||
| CN111057148A | 2020-04-24 | |||
| CN113667014A | 2021-11-19 | |||
| CN112694531A | 2021-04-23 | |||
| CN111018985A | 2020-04-17 | |||
| CN108341870A | 2018-07-31 |
Other References:
H M GREY: "Studies on Changes in the Quality of Rabbit-Bovine Serum Albumin Antibody following Immunization*", IMMUNOLOGY, vol. 7, 1 January 1964 (1964-01-01), pages 82 - 90, XP093169010
MOREL, G. A. ET AL.: "MONOCLONAL ANTIBODIES TO BOVINE SERUM ALBUMIN: AFFINITY AND SPECIFICITY DETERMINATIONS", MOLECULAR IMMUNOLOGY, vol. 25, no. 1, 31 December 1988 (1988-12-31), pages 7 - 15, XP023795534, DOI: 10.1016/0161-5890(88)90085-5
YU, JIAN ET AL.: "Preparation and Characterization of Monoclonal Antibody (McAb) against BSA", PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY, vol. 35, no. 1, 28 February 2007 (2007-02-28), pages 30 - 34
MOREL, G. A. ET AL.: "MONOCLONAL ANTIBODIES TO BOVINE SERUM ALBUMIN: AFFINITY AND SPECIFICITY DETERMINATIONS", MOLECULAR IMMUNOLOGY, vol. 25, no. 1, 31 December 1988 (1988-12-31), pages 7 - 15, XP023795534, DOI: 10.1016/0161-5890(88)90085-5
YU, JIAN ET AL.: "Preparation and Characterization of Monoclonal Antibody (McAb) against BSA", PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY, vol. 35, no. 1, 28 February 2007 (2007-02-28), pages 30 - 34
Attorney, Agent or Firm:
NANJING ZHENGLIAN INTELLECTUALPROPERTY AGENCY CO., LTD. (CN)
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