Title:
ANTI-FXII NANOBODY OR ANTIGEN BINDING FRAGMENT THEREOF AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/253662
Kind Code:
A1
Abstract:
Provided is a nanobody for resisting an FXII specific region or an antigen binding fragment thereof. The nanobody or the antigen binding fragment thereof is combined with FXII by means of a binding epitope of FXII to block the activation of FXII; and the binding epitope comprises a conformational epitope. Further provided is an FXII neutralizing antibody which is screened by means of phage display technology; and it has been found that the FXII neutralizing antibody can treat thrombi, myocardial ischemia reperfusion injuries, and vasculitis in mouse arterial thrombus, rat extracorporeal membrane oxygenation, and mouse myocardial ischemia reperfusion injury and vasculitis models.
Inventors:
WANG MIAO (CN)
XU PENGFEI (CN)
XU PENGFEI (CN)
Application Number:
PCT/CN2020/116566
Publication Date:
December 23, 2021
Filing Date:
September 21, 2020
Export Citation:
Assignee:
NINGBO COMGEN BIOTECH CO LTD (CN)
International Classes:
C07K16/36; A61K38/55; A61K38/57; A61K39/395; A61P7/02; A61P9/10
Domestic Patent References:
| WO2019246176A1 | 2019-12-26 |
Foreign References:
| CN103068845A | 2013-04-24 | |||
| CN103582492A | 2014-02-12 | |||
| CN104080470A | 2014-10-01 | |||
| CN101102819A | 2008-01-09 | |||
| CN103415301A | 2013-11-27 | |||
| CN103687878A | 2014-03-26 |
Other References:
MATAFONOV, A. ET AL.: "Factor XII Inhibition Reduces Thrombus Formation in a Primate Thrombosis Model", BLOOD, vol. 123, no. 11, 13 March 2014 (2014-03-13), XP055393796, ISSN: 0006-4971, DOI: 10.1182/blood-2013-04-
STEVEN, D.M. ET AL.: "Plasmin is a Natural Trigger for Bradykinin Production in Patients with Hereditary Angioedema with Factor XII Mutations", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 138, no. 5, 6 April 2016 (2016-04-06), pages 1423, XP029802669, ISSN: 0091-6749, DOI: 10.1016/j.jaci.2016.02.021
STEVEN, D.M. ET AL.: "A Nanobody-Based Method for Tracking Factor XII Activation in Plasma", THROMB HAEMOST, vol. 110, no. 3, 24 January 2013 (2013-01-24), XP055278398, ISSN: 0340-6245, DOI: 10.1160/TH12-11-0792
STEVEN, D.M. ET AL.: "Plasmin is a Natural Trigger for Bradykinin Production in Patients with Hereditary Angioedema with Factor XII Mutations", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 138, no. 5, 6 April 2016 (2016-04-06), pages 1423, XP029802669, ISSN: 0091-6749, DOI: 10.1016/j.jaci.2016.02.021
STEVEN, D.M. ET AL.: "A Nanobody-Based Method for Tracking Factor XII Activation in Plasma", THROMB HAEMOST, vol. 110, no. 3, 24 January 2013 (2013-01-24), XP055278398, ISSN: 0340-6245, DOI: 10.1160/TH12-11-0792
Attorney, Agent or Firm:
PERIODIC LAW FIRM (CN)
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