Title:
ANTI-VHH DOMAIN ANTIBODIES AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/057978
Kind Code:
A1
Abstract:
The invention provides a group of anti-VHH domain antibodies and use thereof. The present invention further provides use of the described antibodies in the development, screening and purification of nano-antibodies, and use of the described antibodies in the field of immunotherapy.
Inventors:
QIN XIJIAN (CN)
SUN LIWEI (CN)
WANG WANYI (CN)
SONG GUANGWEI (CN)
SUN LIWEI (CN)
WANG WANYI (CN)
SONG GUANGWEI (CN)
Application Number:
PCT/CN2020/118150
Publication Date:
April 01, 2021
Filing Date:
September 27, 2020
Export Citation:
Assignee:
NANJING GENSCRIPT BIOTECH CO LTD (CN)
International Classes:
C07K16/28; C07K16/46; C12N15/13
Domestic Patent References:
| WO1993016185A2 | 1993-08-19 | |||
| WO1993001161A1 | 1993-01-21 | |||
| WO1993008829A1 | 1993-05-13 | |||
| WO2009089004A1 | 2009-07-16 |
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Other References:
ANONYMOUS: "MonoRabTM Rabbit Anti-Camelid VHH Antibody [iFluor488], MAb", GENSCRIPT, 5 May 2017 (2017-05-05), pages 1 - 4, XP009527052
ANONYMOUS: "Jackson alpaca family-nanobody VHH newly launched", 24 September 2019 (2019-09-24), XP055793840, Retrieved from the Internet
ANONYMOUS: "Accelerating Nanobody Production Using Polyclonal Anti-VHH Antibodiessing Polyclonal Anti-VHH Antibodies", 13 September 2019 (2019-09-13), XP055793842, Retrieved from the Internet
C. HAMERS ET AL.: "Naturally occurring antibodies devoid of light chains", NATURE, vol. 363, 1993, pages 466 - 468
CLACKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 3242
CHOTHIALESK, J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
MACCALLUM ET AL., J. MOL. BIOL., vol. 262, 1996, pages 732 - 745
"Kuby Immunology", 2007, W.H. FREEMAN AND CO., pages: 91
PORTOLANO ET AL., J. IMMUNOL., vol. 151, 1993, pages 2623 - 887
CHEN ET AL., J. MOL. BIOL., vol. 293, 1999, pages 865 - 881
PRESTA ET AL., CANCER RES., vol. 57, 1997, pages 4593 - 4599
HUDSON ET AL., NAT. MED., vol. 9, 2003, pages 129 - 134
WINTER ET AL., ANN. REV. IMMUNOL., vol. 113, 1994, pages 433 - 455
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MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 6851 - 6855
ALMAGROFRANSSON, FRONT. BIOSCI., vol. 13, 2008, pages 1619 - 1633
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323 - 329
QUEEN ET AL., PROC. NAT'L ACAD. SCI. USA, vol. 86, 1989, pages 10029 - 10033
DALL'ACQUA ET AL., METHODS, vol. 36, 2005, pages 61 - 68
PADLAN, MOL. IMMUNOL., vol. 28, 1991, pages 489 - 498
KLIMKA ET AL., BR. J. CANCER, vol. 83, 2000, pages 252 - 260
CARTER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 4285
BACA ET AL., J. BIOL. CHEM., vol. 272, 1997, pages 10678 - 10684
ROSOK ET AL., J. BIOL. CHEM., vol. 271, 1996, pages 22611 - 22618
VAN DIJKVAN DE WINKEL, CURR. OPIN. PHARMACOL., vol. 5, 2001, pages 368 - 74
LONBERG, CURR. OPIN. IMMUNOL., vol. 20, 2008, pages 450 - 459
LONBERG, NAT. BIOTECH., vol. 23, 2005, pages 1117 - 1125
KOZBOR, J. IMMUNOL., vol. 133, 1984, pages 3001
BRODEUR ET AL.: "Monoclonal Antibody Production Techniques and Applications", 1987, MARCEL DEKKER, INC., pages: 51 - 63
BOERNER ET AL., J. IMMUNOL., vol. 147, 1991, pages 60
LI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 103, 2006, pages 3557 - 3562
NI, XIANDAI MIANYIXUE, vol. 26, no. 4, 2006, pages 265 - 268
VOLLMERSBRANDLEIN, HISTOLOGY AND HISTOPATHOLOGY, vol. 20, no. 3, 2005, pages 927 - 937
VOLLMERSBRANDLEIN, METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, vol. 27, no. 3, 2005, pages 185 - 91
HOOGENBOOMO'BRIEN ET AL.: "Methods in Molecular Biology", vol. 248, 1996, HUMANA PRESS, article "Epitope Mapping Protocols", pages: 255 - 268
MCCAFFERTY ET AL., NATURE, vol. 305, 1983, pages 537 - 554
HOOGENBOOMWINTER, J. MOL. BIOL., vol. 227, 1992, pages 381 - 388
SIDHU ET AL., J. MOL. BIOL., vol. 340, no. 5, 2004, pages 1073 - 1093
FELLOUSE, PROC. NATL. ACAD. SCI. USA, vol. 101, no. 34, 2004, pages 12467 - 12472
LEE ET AL., J. IMMUNOL. METHODS, vol. 284, no. 1-2, 2004, pages 119 - 132
GRIFFITHS ET AL., EMBO J, vol. 12, 1993, pages 725 - 734
TRAUNECKER ET AL., EMBO J., vol. 10, 1991, pages 3655
BRENNAN ET AL., SCIENCE, vol. 229, 1985, pages 81
KOSTELNY ET AL., J. IMMUNOL., vol. 148, no. 5, 1992, pages 1547 - 1553
GRUBER ET AL., J. IMMUNOL., vol. 152, 1994, pages 5368
CHOWDHURY, METHODS MOL. BIOL., vol. 207, 2008, pages 179 - 196
CUNNINGHAMWELLS, SCIENCE, vol. 244, 1989, pages 1081 - 1085
GERNGROSS, NAT. BIOTECH., vol. 22, 2004, pages 1409 - 1414
LI ET AL., NAT. BIOTECH., vol. 24, 2006, pages 210 - 215
GRAHAM ET AL., J. GEN VIROL., vol. 36, 1977, pages 59
MATHER, BIOL. REPROD., vol. 23, 1980, pages 243 - 251
MATHER ET AL., ANNALS N.Y. ACAD. SCI., vol. 383, 1982, pages 44 - 68
URLAUB ET AL., PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216
HARLOWLANE: "Antibodies: A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
See also references of EP 4036116A4
ANONYMOUS: "Jackson alpaca family-nanobody VHH newly launched", 24 September 2019 (2019-09-24), XP055793840, Retrieved from the Internet
ANONYMOUS: "Accelerating Nanobody Production Using Polyclonal Anti-VHH Antibodiessing Polyclonal Anti-VHH Antibodies", 13 September 2019 (2019-09-13), XP055793842, Retrieved from the Internet
C. HAMERS ET AL.: "Naturally occurring antibodies devoid of light chains", NATURE, vol. 363, 1993, pages 466 - 468
CLACKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 3242
CHOTHIALESK, J. MOL. BIOL., vol. 196, 1987, pages 901 - 917
MACCALLUM ET AL., J. MOL. BIOL., vol. 262, 1996, pages 732 - 745
"Kuby Immunology", 2007, W.H. FREEMAN AND CO., pages: 91
PORTOLANO ET AL., J. IMMUNOL., vol. 151, 1993, pages 2623 - 887
CHEN ET AL., J. MOL. BIOL., vol. 293, 1999, pages 865 - 881
PRESTA ET AL., CANCER RES., vol. 57, 1997, pages 4593 - 4599
HUDSON ET AL., NAT. MED., vol. 9, 2003, pages 129 - 134
WINTER ET AL., ANN. REV. IMMUNOL., vol. 113, 1994, pages 433 - 455
HOLLINGER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 6444 - 6448
MORRISON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 6851 - 6855
ALMAGROFRANSSON, FRONT. BIOSCI., vol. 13, 2008, pages 1619 - 1633
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323 - 329
QUEEN ET AL., PROC. NAT'L ACAD. SCI. USA, vol. 86, 1989, pages 10029 - 10033
DALL'ACQUA ET AL., METHODS, vol. 36, 2005, pages 61 - 68
PADLAN, MOL. IMMUNOL., vol. 28, 1991, pages 489 - 498
KLIMKA ET AL., BR. J. CANCER, vol. 83, 2000, pages 252 - 260
CARTER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 4285
BACA ET AL., J. BIOL. CHEM., vol. 272, 1997, pages 10678 - 10684
ROSOK ET AL., J. BIOL. CHEM., vol. 271, 1996, pages 22611 - 22618
VAN DIJKVAN DE WINKEL, CURR. OPIN. PHARMACOL., vol. 5, 2001, pages 368 - 74
LONBERG, CURR. OPIN. IMMUNOL., vol. 20, 2008, pages 450 - 459
LONBERG, NAT. BIOTECH., vol. 23, 2005, pages 1117 - 1125
KOZBOR, J. IMMUNOL., vol. 133, 1984, pages 3001
BRODEUR ET AL.: "Monoclonal Antibody Production Techniques and Applications", 1987, MARCEL DEKKER, INC., pages: 51 - 63
BOERNER ET AL., J. IMMUNOL., vol. 147, 1991, pages 60
LI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 103, 2006, pages 3557 - 3562
NI, XIANDAI MIANYIXUE, vol. 26, no. 4, 2006, pages 265 - 268
VOLLMERSBRANDLEIN, HISTOLOGY AND HISTOPATHOLOGY, vol. 20, no. 3, 2005, pages 927 - 937
VOLLMERSBRANDLEIN, METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY, vol. 27, no. 3, 2005, pages 185 - 91
HOOGENBOOMO'BRIEN ET AL.: "Methods in Molecular Biology", vol. 248, 1996, HUMANA PRESS, article "Epitope Mapping Protocols", pages: 255 - 268
MCCAFFERTY ET AL., NATURE, vol. 305, 1983, pages 537 - 554
HOOGENBOOMWINTER, J. MOL. BIOL., vol. 227, 1992, pages 381 - 388
SIDHU ET AL., J. MOL. BIOL., vol. 340, no. 5, 2004, pages 1073 - 1093
FELLOUSE, PROC. NATL. ACAD. SCI. USA, vol. 101, no. 34, 2004, pages 12467 - 12472
LEE ET AL., J. IMMUNOL. METHODS, vol. 284, no. 1-2, 2004, pages 119 - 132
GRIFFITHS ET AL., EMBO J, vol. 12, 1993, pages 725 - 734
TRAUNECKER ET AL., EMBO J., vol. 10, 1991, pages 3655
BRENNAN ET AL., SCIENCE, vol. 229, 1985, pages 81
KOSTELNY ET AL., J. IMMUNOL., vol. 148, no. 5, 1992, pages 1547 - 1553
GRUBER ET AL., J. IMMUNOL., vol. 152, 1994, pages 5368
CHOWDHURY, METHODS MOL. BIOL., vol. 207, 2008, pages 179 - 196
CUNNINGHAMWELLS, SCIENCE, vol. 244, 1989, pages 1081 - 1085
GERNGROSS, NAT. BIOTECH., vol. 22, 2004, pages 1409 - 1414
LI ET AL., NAT. BIOTECH., vol. 24, 2006, pages 210 - 215
GRAHAM ET AL., J. GEN VIROL., vol. 36, 1977, pages 59
MATHER, BIOL. REPROD., vol. 23, 1980, pages 243 - 251
MATHER ET AL., ANNALS N.Y. ACAD. SCI., vol. 383, 1982, pages 44 - 68
URLAUB ET AL., PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216
HARLOWLANE: "Antibodies: A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
See also references of EP 4036116A4
Attorney, Agent or Firm:
WU, FENG & ZHANG (CN)
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