Title:
ANTIBACTERIAL PEPTIDE P104 AND LYASE LYSP53 HAVING BROAD-SPECTRUM CRACKING ACTIVITY, AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/279983
Kind Code:
A1
Abstract:
Provided are an antibacterial peptide P104 and a lyase LysP53 having broad-spectrum cracking activity, and applications thereof. An amino acid sequence of the antibacterial peptide P104 is as shown in SEQ ID NO.1, and a gene sequence of the antibacterial peptide P104 is as shown in SEQ ID NO.3; an amino acid sequence of the lyase LysP53 is as shown in SEQ ID NO.2, and a gene sequence of the lyase LysP53 is as shown in SEQ ID NO.4; the antibacterial peptide P104 and the lyase LysP53 have a relatively good cracking effect on Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Eescherichia coli in vitro, and can be used for cracking Gram-negative bacteria in a broad-spectrum manner; meanwhile, the lyase LysP53 can be subjected to soluble expression in Escherichia coli, and the enzyme activity is high, and thus, the antibacterial peptide P104 and the lyase LysP53 have relatively good application prospects in research and development of anti-infection drugs.
Inventors:
WEI HONGPING (CN)
YANG HANG (CN)
LI CHANGCHANG (CN)
YU JUNPING (CN)
JIANG MENGWEI (CN)
YANG HANG (CN)
LI CHANGCHANG (CN)
YU JUNPING (CN)
JIANG MENGWEI (CN)
Application Number:
PCT/CN2022/100968
Publication Date:
January 12, 2023
Filing Date:
June 24, 2022
Export Citation:
Assignee:
WUHAN INST VIROLOGY CAS (CN)
International Classes:
C12N9/88; A61K38/51; A61P31/04; C12N1/21; C12N15/60; C12N15/70; C12R1/19
Domestic Patent References:
| WO2010034783A1 | 2010-04-01 |
Foreign References:
| CN113549610A | 2021-10-26 | |||
| CN111235119A | 2020-06-05 | |||
| CN110330553A | 2019-10-15 | |||
| CN106854247A | 2017-06-16 | |||
| US20090312248A1 | 2009-12-17 | |||
| US20180104316A1 | 2018-04-19 |
Other References:
DATABASE PROTEIN ANONYMOUS : "endopeptidase [Acinetobacter phage DMU1]", XP093022738, retrieved from NCBI
KONASANI VENKAT RAO, JIN CHUNSHENG, KARLSSON NICLAS G., ALBERS EVA: "A novel ulvan lyase family with broad-spectrum activity from the ulvan utilisation loci of Formosa agariphila KMM 3901", SCIENTIFIC REPORTS, vol. 8, no. 1, XP093022736, DOI: 10.1038/s41598-018-32922-0
"National Center for Biotechnology Information", Database accession no. MW590698
KONASANI VENKAT RAO, JIN CHUNSHENG, KARLSSON NICLAS G., ALBERS EVA: "A novel ulvan lyase family with broad-spectrum activity from the ulvan utilisation loci of Formosa agariphila KMM 3901", SCIENTIFIC REPORTS, vol. 8, no. 1, XP093022736, DOI: 10.1038/s41598-018-32922-0
"National Center for Biotechnology Information", Database accession no. MW590698
Attorney, Agent or Firm:
SHENZHEN FENGCHENGZHIHE IP ATTORNEY LTD (CN)
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