Title:
ANTIBODY AGAINST AFLATOXINS, SUPPORT USING THE ANTIBODY, METHOD OF IMMUNOLOGICALLY DETECTING AFLATOXINS AND METHOD OF CONCENTRATING AND PURIFYING AFLATOXINS
Document Type and Number:
WIPO Patent Application WO/2008/059837
Kind Code:
A1
Abstract:
It is intended to detect, concentrate and purify aflatoxins of all types which are possibly contained in a sample such as a food. It is also intended to detect the total amount or the individual amounts thereof at a high sensitivity. By using aflatoxin B2 or its derivative as a hapten compound, an antibody, which shows the same reactivity to individual aflatoxin analogs and is highly tolerant to organic solvents, is obtained. Then, a detection/concentration/purification means and an immunological detection means with the use of the above antibody are constructed. The detection means thus constructed achieves a high sensitivity and excellent quantification properties.
Inventors:
UCHIGASHIMA MIKIKO (JP)
MIYAKE SHIRO (JP)
YAMASHITA HIROSHI (JP)
MIYAKE SHIRO (JP)
YAMASHITA HIROSHI (JP)
Application Number:
PCT/JP2007/072010
Publication Date:
May 22, 2008
Filing Date:
November 13, 2007
Export Citation:
Assignee:
HORIBA LTD (JP)
UCHIGASHIMA MIKIKO (JP)
MIYAKE SHIRO (JP)
YAMASHITA HIROSHI (JP)
UCHIGASHIMA MIKIKO (JP)
MIYAKE SHIRO (JP)
YAMASHITA HIROSHI (JP)
International Classes:
C07K16/14; C07D493/14; C07D493/22; C07K17/08; C12N5/10; C12P21/08; G01N33/02
Foreign References:
| JP2001187800A | 2001-07-10 | |||
| US4835100A | 1989-05-30 | |||
| JPS63219394A | 1988-09-13 | |||
| JPH04360695A | 1992-12-14 |
Other References:
HASTINGS K.L. ET AL.: "Production and characterization of a monoclonal antibody to alfatoxin B2", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 36, no. 2, 1988, pages 404 - 408, XP003022644
CANDLISH J. E. ET AL., LETTERS IN APPL. MICROBIOL., vol. 1, 1985, pages 57
GROOPMAN ET AL., PROC. NATL. ACAD. SCI., vol. 81, 1984, pages 7728
HERTZOG P. J. ET AL., CARCINOGENESIS, vol. 3, 1982, pages 825 - 828
CHU, F. S.; HSIA, M-T. S.; SUN. P., J. ASSOC. OFF. ANAL. CHEM., vol. 60, 1977, pages 791
A. E. KARU ET AL., J. AGRIC. FOOD CHEM., vol. 42, 1994, pages 301 - 309
B. F. ERLANGERET ET AL., J. BIOL. CHEM., vol. 234, 1954, pages 1090 - 1094
AUSUBEL ET AL.: "Current Protocols in Molecular Biology edit.", 1987, JOHN WILEY AND SONS, pages: 11.4 - 11.11
METH. ENZYMOL., vol. 92, 1983, pages 147 - 523
"Antibodies", vol. II, 1989, IRL PRESS
See also references of EP 2090590A4
CANDLISH J. E. ET AL., LETTERS IN APPL. MICROBIOL., vol. 1, 1985, pages 57
GROOPMAN ET AL., PROC. NATL. ACAD. SCI., vol. 81, 1984, pages 7728
HERTZOG P. J. ET AL., CARCINOGENESIS, vol. 3, 1982, pages 825 - 828
CHU, F. S.; HSIA, M-T. S.; SUN. P., J. ASSOC. OFF. ANAL. CHEM., vol. 60, 1977, pages 791
A. E. KARU ET AL., J. AGRIC. FOOD CHEM., vol. 42, 1994, pages 301 - 309
B. F. ERLANGERET ET AL., J. BIOL. CHEM., vol. 234, 1954, pages 1090 - 1094
AUSUBEL ET AL.: "Current Protocols in Molecular Biology edit.", 1987, JOHN WILEY AND SONS, pages: 11.4 - 11.11
METH. ENZYMOL., vol. 92, 1983, pages 147 - 523
"Antibodies", vol. II, 1989, IRL PRESS
See also references of EP 2090590A4
Attorney, Agent or Firm:
OZAKI, Yuzo et al. (13-9 Nishinakajima 5-chome,Yodogawa-ku, Osaka-shi, Osaka 11, JP)
Download PDF:
Previous Patent: VEHICLE STEERING DEVICE
Next Patent: TIRE WITH SENSOR AND METHOD FOR MEASURING TIRE DISTORTION LEVEL
Next Patent: TIRE WITH SENSOR AND METHOD FOR MEASURING TIRE DISTORTION LEVEL