Title:
ANURAN CROSS-SPECIES MOLECULAR SENSORS
Document Type and Number:
WIPO Patent Application WO/2015/044932
Kind Code:
A3
Abstract:
Described herein are DNA primer sequences designed for the determination of gene or transcript information from Anuran species, and which may be used in studies for developmental and/or toxicity testing and for environmental toxicology or ecological assessment. Also described herein is a rapid, sensitive, high-throughput assay useful for supporting potential risk assessment across vertebrate clades, and that is also useful for evaluation of complex contaminant mixtures.
Inventors:
PROPPER CATHERINE R (US)
BECKSTROM-STERNBERG STEPHEN M (US)
HELBING CAREN C (CA)
VELDHOEN NICHOLAS J (CA)
BECKSTROM-STERNBERG STEPHEN M (US)
HELBING CAREN C (CA)
VELDHOEN NICHOLAS J (CA)
Application Number:
PCT/IB2014/065159
Publication Date:
August 06, 2015
Filing Date:
October 09, 2014
Export Citation:
Assignee:
UNIV ARIZONA (US)
International Classes:
G01N33/50; C12Q1/68; G01N33/78
Other References:
SKIRROW, RC ET AL.: "Roscovitine Inhibits Thyroid Hormone-Induced Tail Regression Of The Frog Tadpole And Reveals A Role For Cyclin C/Cdk8 In The Establishment Of The Metamorphic Gene Expression Program.", DEVELOPMENTAL DYMANICS., vol. 237, no. 12, 26 November 2008 (2008-11-26), pages 378 - 3797, XP055217468
DATABASE GENBANK 1 November 1996 (1996-11-01), ASAHINA, K.: "Cloning And Characterization Of The Full Length cDNA Encoding Alpha2 Type I Collagen Of Bullfrog Rana Catesbeiana: Rana Catesbeiana mRNA For Alpha 2 Type I Collagen, Complete cds.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/D88764 accession no. 88764.
DATABASE NCBI 7 April 2015 (2015-04-07), JUNG, JC ET AL.: "Matrix Metalloproeinases Mediate The Dismantling Of Mesenchymal Structures In The Tadpole Tail During Thyroid Hormone-Induced Tail Resporption: Xenopus laevis Matrix Metallopeptidase 2 (mmp2), mRNA", retrieved from http://www.ncbi.nlm.nih.gov/nucleotide/148231335 ? report=genbank&log$=nuclalign&blast_rank=21&RID=J8AS5TYD01R accession no. M_001087228.1. 2002 ;
DATABASE NCBI 7 April 2015 (2015-04-07), SHI, YB ET AL.: "Xenopus laevis Ribosomal Protein L8 (rpl8), mRNA", retrieved from http://www.ncbi.nlm.nih.gov/nucleotide/147900068?report=genbank&log $=nuclalign&blast-rank=39&RID=J8CGZXV3016 accession no. M_001086996.1
VELDHOEN, N ET AL.: "Enabling Comparative Gene Expression Studies Of Thyroid Hormoneaction Through The Development Of A Flexible Real-Time Quantitative PCR Assay For Use Across Multiple Anuran Indicator Andsentinel Species.", AQUATIC TOXICOLOGY., vol. 148, 2014, pages 162 - 173
DATABASE GENBANK 1 November 1996 (1996-11-01), ASAHINA, K.: "Cloning And Characterization Of The Full Length cDNA Encoding Alpha2 Type I Collagen Of Bullfrog Rana Catesbeiana: Rana Catesbeiana mRNA For Alpha 2 Type I Collagen, Complete cds.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/D88764 accession no. 88764.
DATABASE NCBI 7 April 2015 (2015-04-07), JUNG, JC ET AL.: "Matrix Metalloproeinases Mediate The Dismantling Of Mesenchymal Structures In The Tadpole Tail During Thyroid Hormone-Induced Tail Resporption: Xenopus laevis Matrix Metallopeptidase 2 (mmp2), mRNA", retrieved from http://www.ncbi.nlm.nih.gov/nucleotide/148231335 ? report=genbank&log$=nuclalign&blast_rank=21&RID=J8AS5TYD01R accession no. M_001087228.1. 2002 ;
DATABASE NCBI 7 April 2015 (2015-04-07), SHI, YB ET AL.: "Xenopus laevis Ribosomal Protein L8 (rpl8), mRNA", retrieved from http://www.ncbi.nlm.nih.gov/nucleotide/147900068?report=genbank&log $=nuclalign&blast-rank=39&RID=J8CGZXV3016 accession no. M_001086996.1
VELDHOEN, N ET AL.: "Enabling Comparative Gene Expression Studies Of Thyroid Hormoneaction Through The Development Of A Flexible Real-Time Quantitative PCR Assay For Use Across Multiple Anuran Indicator Andsentinel Species.", AQUATIC TOXICOLOGY., vol. 148, 2014, pages 162 - 173
Attorney, Agent or Firm:
ROSENFIELD, Susan Stone (P.C.2394 East Camelback Road, Suite 60, Phoenix Arizona, US)
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