Title:
APPARATUS FOR MEASURING FLUORESCENCE SIGNALS IN PLURALITY OF ILLUMINATION MODES, MEASUREMENT METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/228388
Kind Code:
A1
Abstract:
Disclosed is an apparatus for measuring fluorescence signals in a plurality of illumination modes. Excitation light is modulated in an illumination excitation light path; excitation illumination patterns with different phases are generated at a sample (32) after the excitation light passes through an objective lens (31); a high-speed switching apparatus is provided in a fluorescence collection light path to switch the position, on a target surface of a photoelectric sensor (40), of a fluorescent image of the sample (32); and by means of the simultaneous operation of a plurality of illumination excitation light paths and a plurality of fluorescence imaging light paths, a plurality of sub-images, i.e. fluorescence signals corresponding to a plurality of illumination modes, can be simultaneously obtained after a single instance of exposure. Fluorescence signal measurement of a single molecule can be realized in the plurality of illumination modes, the precision of positioning a single molecule can be improved, and a higher resolution capability can be realized under the condition of having an equal number of photons. Further disclosed are a measurement method for the apparatus for measuring fluorescence signals in a plurality of illumination modes, and an application thereof.
Inventors:
GU LUSHENG (CN)
JI WEI (CN)
XU TAO (CN)
FU YANHUI (CN)
JI WEI (CN)
XU TAO (CN)
FU YANHUI (CN)
Application Number:
PCT/CN2020/076033
Publication Date:
November 19, 2020
Filing Date:
February 20, 2020
Export Citation:
Assignee:
INST BIOPHYSICS CAS (CN)
International Classes:
G02B21/00; G01N21/01; G01N21/64; G02B21/36
Foreign References:
| CN110007453A | 2019-07-12 | |||
| CN209895085U | 2020-01-03 | |||
| CN103605202A | 2014-02-26 | |||
| CN102540446A | 2012-07-04 | |||
| CN105758799A | 2016-07-13 | |||
| US20130314717A1 | 2013-11-28 | |||
| US20160124208A1 | 2016-05-05 |
Attorney, Agent or Firm:
JIAQUAN IP LAW FIRM (CN)
Download PDF: