APPLICATION OF HEAT-RESISTANT β-GLUCOSIDASE IN PREPARATION OF GENTIOOLIGOSACCHARIDE

Title:

APPLICATION OF HEAT-RESISTANT β-GLUCOSIDASE IN PREPARATION OF GENTIOOLIGOSACCHARIDE

Document Type and Number:

WIPO Patent Application WO/2021/217960

Kind Code:

A1

Abstract:

An application of heat-resistant β-glucosidase TSBGl in preparation of gentiooligosaccharide. The heat-resistant β-glucosidase TSBGl from Thermotoga sp.KOL6 takes Bacillus subtilis WSH11 as an expression host, so that high-efficiency expression of the tsbgl gene in bacillus subtilis is achieved. The optimum temperature of the β-glucosidase TSBGl is 90℃, the optimum pH is 6.0, and the β-glucosidase TSBGl has relatively high thermostability at 90℃. The β-glucosidase is added to a reaction system employing 1200 g/L of glucose as a substrate, an enzyme reaction is carried out under a pH of 6.0 at 90℃, and the yield of the gentiooligosaccharide reaches 178.2 g/L. The β-glucosidase meets requirements for industrial applications such as foods and can be applied to industrial production of the gentiooligosaccharide.

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Inventors:

WU JING (CN)
XIA WEI (CN)
SHENG LINGLING (CN)
HUANG YAN (CN)

Application Number:

PCT/CN2020/109763

Publication Date:

November 04, 2021

Filing Date:

August 18, 2020

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Assignee:

UNIV JIANGNAN (CN)

International Classes:

C12N1/21; C12N15/56; C12N15/75; C12P19/00; C12P19/12; C12P19/14; C12P19/18; C12R1/125

Foreign References:

CN111411117A	2020-07-14
CN107099565A	2017-08-29
CN108102997A	2018-06-01
CN109355275A	2019-02-19

Other References:

DATABASE PROTEIN 11 October 2019 (2019-10-11), ANONYMOUS : "beta-glucosidase [Thermotoga sp. KOL6] ", XP055862845, retrieved from NCBI Database accession no. WP_101510358
WANG FEI, JING WU, SHENG CHEN: "Preparation of gentiooligosaccharides using Trichoderma viride β-glucosidase", FOOD CHEMISTRY, vol. 248, 14 December 2017 (2017-12-14), pages 340 - 345, XP055862844, DOI: 10.1016/j.foodchem.2017.12.044

Attorney, Agent or Firm:

HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)

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