Title:
APPLICATION OF NEONATAL BILIARY ATRESIA BIOMARKER AND DETECTION METHOD FOR SAME
Document Type and Number:
WIPO Patent Application WO/2022/127933
Kind Code:
A1
Abstract:
Disclosed in the present invention is an application of conjugated bilirubin as a biomarker in preparation of a diagnosis device for evaluating a risk of neonatal biliary atresia. Also disclosed in the present invention are a bilirubin content measurement method, a stable quantitative detection reagent for bilirubin, and an application of the quantitative detection reagent for bilirubin in preparation of a kit for measuring a content of the bilirubin and evaluating a risk of biliary atresia of a subject, infant hepatitis syndrome, α1 antitrypsin deficiency disease or Alagille syndrome. Also disclosed in the present invention are a kit for measuring the content of the bilirubin or evaluating the risk of neonatal biliary atresia, an application of the kit, a method for evaluating the risk of neonatal biliary atresia, and a device for predicting the risk of neonatal biliary atresia by using an expression level of the conjugated bilirubin. The present invention has the advantages of being simple and rapid, flexible in sample preparation, low in limit of detection, good in repeatability and high in sensitivity; and the sample preparation and detection method is simple and easy to implement, low in cost and suitable for popularization and use.
Inventors:
JIA WEI (CN)
XIE GUOXIANG (CN)
ZHOU KEJUN (CN)
XIE GUOXIANG (CN)
ZHOU KEJUN (CN)
Application Number:
PCT/CN2021/139607
Publication Date:
June 23, 2022
Filing Date:
December 20, 2021
Export Citation:
Assignee:
HUMAN METABOLOMICS INST INC (CN)
International Classes:
G01N33/72; G01N30/88; G01N33/52
Foreign References:
| CN112748249A | 2021-05-04 | |||
| CN108267585A | 2018-07-10 | |||
| CN112080560A | 2020-12-15 |
Other References:
ITO FUJIO, ANDO HISAMI, WATANABE YOSHIO, ITO TAKAHIRO: "Serum bilirubin fractions in cholestatic pediatric patients: Determination with Micronex high-performance liquid chromatography", JOURNAL OF PEDIATRIC SURGERY, vol. 30, no. 4, 30 March 1993 (1993-03-30), US , pages 596 - 599, XP055943643, ISSN: 0022-3468, DOI: 10.1016/0022-3468(95)90140-X
GYÖRGY LENGYEL, ZSUZSA VERES, PÁL SZABÓ, LÁSZLÓ VERECZKEY , KATALIN JEMNITZ: "Canalicular and sinusoidal disposition of bilirubin mono- and diglucuronides in sandwich-cultured human and rat primary hepatocytes", DRUG METABOLISM AND DISPOSITION, vol. 33, no. 9, 1 September 2005 (2005-09-01), US , pages 1335 - 1360, XP009537661, ISSN: 0090-9556, DOI: 10.1124/dmd.105.004481
ZHENHUA GONG , YIBO WU , LULU ZHENG , LICAI CHEN , ZHIBZO LV: "Can Free Carnitine or Bilirubin in Blood Be Used in Neonatal Screening for Biliary Atresia", EUROPEAN JOURNAL OF PEDIATRIC SURGERY, vol. 30, no. 5, 31 October 2020 (2020-10-31), DE , pages 459 - 464, XP009537662, ISSN: 0939-7248, DOI: 10.1055/s-0039-1698764
HARPAVAT SANJIV, GARCIA-PRATS JOSEPH A., ANAYA CARLOS, BRANDT MARY L., LUPO PHILIP J., FINEGOLD MILTON J., OBUOBI ALICE, ELHENNAWY: "Diagnostic Yield of Newborn Screening for Biliary Atresia Using Direct or Conjugated Bilirubin Measurements", JAMA THE JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, vol. 323, no. 12, 24 March 2020 (2020-03-24), US , pages 1141 - 1150, XP055943650, ISSN: 0098-7484, DOI: 10.1001/jama.2020.0837
WANG QI;ZHANG YU-JIE;DAI ZHONG;MA SHUANG-CHENG: "Establishment of a Method to Determine the Bilirubin and Bilirubin Qlucuronidations in Different Systems", CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 35, no. 9, 30 September 2015 (2015-09-30), pages 1544 - 1550, XP055943653, ISSN: 0254-1793, DOI: 10.16155/j.0254-1793.2015.09.06
GYÖRGY LENGYEL, ZSUZSA VERES, PÁL SZABÓ, LÁSZLÓ VERECZKEY , KATALIN JEMNITZ: "Canalicular and sinusoidal disposition of bilirubin mono- and diglucuronides in sandwich-cultured human and rat primary hepatocytes", DRUG METABOLISM AND DISPOSITION, vol. 33, no. 9, 1 September 2005 (2005-09-01), US , pages 1335 - 1360, XP009537661, ISSN: 0090-9556, DOI: 10.1124/dmd.105.004481
ZHENHUA GONG , YIBO WU , LULU ZHENG , LICAI CHEN , ZHIBZO LV: "Can Free Carnitine or Bilirubin in Blood Be Used in Neonatal Screening for Biliary Atresia", EUROPEAN JOURNAL OF PEDIATRIC SURGERY, vol. 30, no. 5, 31 October 2020 (2020-10-31), DE , pages 459 - 464, XP009537662, ISSN: 0939-7248, DOI: 10.1055/s-0039-1698764
HARPAVAT SANJIV, GARCIA-PRATS JOSEPH A., ANAYA CARLOS, BRANDT MARY L., LUPO PHILIP J., FINEGOLD MILTON J., OBUOBI ALICE, ELHENNAWY: "Diagnostic Yield of Newborn Screening for Biliary Atresia Using Direct or Conjugated Bilirubin Measurements", JAMA THE JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, vol. 323, no. 12, 24 March 2020 (2020-03-24), US , pages 1141 - 1150, XP055943650, ISSN: 0098-7484, DOI: 10.1001/jama.2020.0837
WANG QI;ZHANG YU-JIE;DAI ZHONG;MA SHUANG-CHENG: "Establishment of a Method to Determine the Bilirubin and Bilirubin Qlucuronidations in Different Systems", CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 35, no. 9, 30 September 2015 (2015-09-30), pages 1544 - 1550, XP055943653, ISSN: 0254-1793, DOI: 10.16155/j.0254-1793.2015.09.06
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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