Title:
APTAMER NUCLEIC ACID MOLECULE AND COMPLEX AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/221238
Kind Code:
A1
Abstract:
Provide an aptamer nucleic acid molecule, and a complex containing said aptamer nucleic acid molecule and a small fluorophore molecule; the aptamer nucleic acid molecule is used for detecting RNA, DNA, or other target molecules inside or outside the cell; and a kit containing the aptamer nucleic acid molecule. The aptamer nucleic acid molecule can specifically bind a small fluorophore molecule, and significantly increase its fluorescence intensity under the excitation of light of suitable wavelength.
Inventors:
YANG YI (CN)
ZHU LINYONG (CN)
CHEN XIANJUN (CN)
ZHANG DASHENG (CN)
SU NI (CN)
LIN QIUNING (CN)
ZHU LINYONG (CN)
CHEN XIANJUN (CN)
ZHANG DASHENG (CN)
SU NI (CN)
LIN QIUNING (CN)
Application Number:
PCT/CN2020/087415
Publication Date:
November 05, 2020
Filing Date:
April 28, 2020
Export Citation:
Assignee:
UNIV EAST CHINA SCIENCE & TECH (CN)
International Classes:
C12N15/115; C07C255/32; C09K19/16; C12N15/64; G01N21/64
Foreign References:
| CN102171234A | 2011-08-31 | |||
| CN1764729A | 2006-04-26 | |||
| CN103710030A | 2014-04-09 |
Other References:
XIANJUN CHEN ET AL.: "Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs", NATURE BIOTECHNOLOGY, vol. 37, no. 11,, 23 September 2019 (2019-09-23), XP036920801, ISSN: 1546-1696, DOI: 20200717165531PX
YUICHI FURUHATA ET AL.: "Programmable RNA detection with a fluorescent RNA aptamer using optimized three-way junction formation", RNA, vol. 25, no. 5, 11 February 2019 (2019-02-11), XP055751471, ISSN: 1469-9001, DOI: 20200717164549
KHALID K. ALAM ET AL.: "A Fluorescent Split Aptamer for Visualizing RNA−RNA Assembly In Vivo", ACS SYNTH. BIOL., vol. 6, no. 9, 26 May 2017 (2017-05-26), XP055705833, ISSN: 2161-5063, DOI: 20200717170722A
DING, AIXIANG: "The Applications of Amine α-Cyanostilbene-modified Derivatives in Fluorescence Liquid Crystals and Environmental Sensing", SCIENCE-ENGINEERING (A), CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 9, 15 September 2014 (2014-09-15), pages 1 - 94, XP055857756, ISSN: 1674-0246
OZAWA ET AL., NATURE METHODS, vol. 4, 2007, pages 413 - 419
ARORA ET AL., NUCLEIC ACIDS RESEARCH, vol. 21, 2015, pages e144
PAIGE ET AL., SCIENCE, vol. 333, 2011, pages 642 - 646
STRACK ET AL., NATURE METHODS, vol. 10, 2013, pages 1219 - 1224
PAIGE ET AL., SCIENCE, vol. 335, 2012, pages 1194
SONG ET AL., NATURE CHEMICAL BIOLOGY, vol. 13, 2017, pages 1187 - 1194
HAN ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 135, 2013, pages 19033 - 19038
FILONOV ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 136, 2014, pages 16299 - 16308
NEEDLEMANWUNSCH, J.MOL.BIOL., vol. 48, 1970, pages 443 - 453
"EMBOSS: The European Molecular Biology Open Software Suite, Rice", TRENDS IN GENETICS, vol. 16, 2000, pages 276 - 277
SAMBROOK.JD. W. RUSSELL: "Lab Ref A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench", August 2002, SCIENCE PRESS
FILONOV ET AL., CHEMISTRY & BIOLOGY, vol. 22, 2015, pages 649 - 660
SONG ET AL., NATURE CHEMICAL BIOLOGY, vol. 13, 2017, pages 1187 - 1194
SHAO ET AL., NUCLEIC ACIDS RESEARCH, vol. 44, 2016, pages e86
See also references of EP 3964583A4
YUICHI FURUHATA ET AL.: "Programmable RNA detection with a fluorescent RNA aptamer using optimized three-way junction formation", RNA, vol. 25, no. 5, 11 February 2019 (2019-02-11), XP055751471, ISSN: 1469-9001, DOI: 20200717164549
KHALID K. ALAM ET AL.: "A Fluorescent Split Aptamer for Visualizing RNA−RNA Assembly In Vivo", ACS SYNTH. BIOL., vol. 6, no. 9, 26 May 2017 (2017-05-26), XP055705833, ISSN: 2161-5063, DOI: 20200717170722A
DING, AIXIANG: "The Applications of Amine α-Cyanostilbene-modified Derivatives in Fluorescence Liquid Crystals and Environmental Sensing", SCIENCE-ENGINEERING (A), CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 9, 15 September 2014 (2014-09-15), pages 1 - 94, XP055857756, ISSN: 1674-0246
OZAWA ET AL., NATURE METHODS, vol. 4, 2007, pages 413 - 419
ARORA ET AL., NUCLEIC ACIDS RESEARCH, vol. 21, 2015, pages e144
PAIGE ET AL., SCIENCE, vol. 333, 2011, pages 642 - 646
STRACK ET AL., NATURE METHODS, vol. 10, 2013, pages 1219 - 1224
PAIGE ET AL., SCIENCE, vol. 335, 2012, pages 1194
SONG ET AL., NATURE CHEMICAL BIOLOGY, vol. 13, 2017, pages 1187 - 1194
HAN ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 135, 2013, pages 19033 - 19038
FILONOV ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 136, 2014, pages 16299 - 16308
NEEDLEMANWUNSCH, J.MOL.BIOL., vol. 48, 1970, pages 443 - 453
"EMBOSS: The European Molecular Biology Open Software Suite, Rice", TRENDS IN GENETICS, vol. 16, 2000, pages 276 - 277
SAMBROOK.JD. W. RUSSELL: "Lab Ref A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench", August 2002, SCIENCE PRESS
FILONOV ET AL., CHEMISTRY & BIOLOGY, vol. 22, 2015, pages 649 - 660
SONG ET AL., NATURE CHEMICAL BIOLOGY, vol. 13, 2017, pages 1187 - 1194
SHAO ET AL., NUCLEIC ACIDS RESEARCH, vol. 44, 2016, pages e86
See also references of EP 3964583A4
Attorney, Agent or Firm:
SHANGHAI ESSEN PATENT & TRADEMARK OFFICE (CN)
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