Title:
ARTIFICIAL MIMIC MIRNA FOR CONTROLLING GENE EXPRESSION, AND USE OF SAME
Document Type and Number:
WIPO Patent Application WO/2015/099122
Kind Code:
A1
Abstract:
Provided is novel artificial mimic miRNA utilizing miRNA. Single-stranded nucleic acid in which an X region and a Y region bind, the X region being a mature miRNA guide strand sequence or a partial sequence thereof and comprising a binding side region (XB) for binding to the Y region and a non-binding side region (XF), the binding side region (XB) being a sequence that does not undergo intramolecular annealing within this region, the Y region being a sequence that undergoes intramolecular annealing with the non-binding side region (XF) of the X region, is used as artificial mimic miRNA. This artificial mimic miRNA makes it possible to control the expression of a target gene.
Inventors:
KURODA MASAHIKO (JP)
OHNO SHINICHIRO (JP)
OHNO SHINICHIRO (JP)
Application Number:
PCT/JP2014/084508
Publication Date:
July 02, 2015
Filing Date:
December 26, 2014
Export Citation:
Assignee:
UNIV TOKYO MEDICAL (JP)
International Classes:
C12N15/113; A61K31/7105; A61P35/00
Domestic Patent References:
| WO2010056737A2 | 2010-05-20 |
Foreign References:
| US20130179999A1 | 2013-07-11 |
Other References:
MCMANUS M.T. ET AL.: "Gene silencing using micro-RNA designed hairpins", RNA, vol. 8, no. 6, 1 June 2002 (2002-06-01), pages 842 - 850, XP002296480, DOI: 10.1017/S1355838202024032
WINTER J. ET AL.: "Loop-miRs: active microRNAs generated from single-stranded loop regions", NUCLEIC ACIDS RES., vol. 41, no. 10, 1 January 2013 (2013-01-01), pages 5503 - 5512, XP055354141, DOI: 10.1093/NAR/GKT251
See also references of EP 3088524A4
DEITERS, THE AAPS JOURNAL, vol. 12, 2009, pages 51 - 60
TAKESHITA ET AL., MOL. THER., vol. 18, 2010, pages 181 - 187
JOHNSON ET AL., NATURE, vol. 410, 26 April 2001 (2001-04-26), pages 1111
WINTER J. ET AL.: "Loop-miRs: active microRNAs generated from single-stranded loop regions", NUCLEIC ACIDS RES., vol. 41, no. 10, 1 January 2013 (2013-01-01), pages 5503 - 5512, XP055354141, DOI: 10.1093/NAR/GKT251
See also references of EP 3088524A4
DEITERS, THE AAPS JOURNAL, vol. 12, 2009, pages 51 - 60
TAKESHITA ET AL., MOL. THER., vol. 18, 2010, pages 181 - 187
JOHNSON ET AL., NATURE, vol. 410, 26 April 2001 (2001-04-26), pages 1111
Attorney, Agent or Firm:
TSUJIMARU Koichiro et al. (JP)
Koichiro Tsujimaru (JP)
Koichiro Tsujimaru (JP)
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