Title:
ASPARTATE KINASE GENE EXPRESSION REGULATORY SEQUENCE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/199460
Kind Code:
A1
Abstract:
Disclosed are an aspartate kinase gene expression regulatory sequence and the use thereof. By modifying positions 366-373 of a sequence represented by SEQ ID NO: 1, a polynucleotide formed by linking the sequence with a start codon GTG or TTG has transcriptional expression regulation activity, and these polynucleotides can improve expression of the aspartate kinase encoding gene lysC, such that a large amount of L-lysine is accumulated.
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Inventors:
SUN JIBIN (CN)
LIU JIAO (CN)
ZHENG PING (CN)
SHI TUO (CN)
ZHOU WENJUAN (CN)
MA YANHE (CN)
LIU JIAO (CN)
ZHENG PING (CN)
SHI TUO (CN)
ZHOU WENJUAN (CN)
MA YANHE (CN)
Application Number:
PCT/CN2022/081527
Publication Date:
September 29, 2022
Filing Date:
March 17, 2022
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N1/21; C12N15/113; C12N9/12; C12N15/67; C12N15/77; C12P7/44; C12P13/00; C12P13/06; C12P13/08; C12P13/12; C12R1/15
Domestic Patent References:
| WO2017100376A2 | 2017-06-15 |
Foreign References:
| CN112695036A | 2021-04-23 | |||
| CN110951662A | 2020-04-03 | |||
| CN105849249A | 2016-08-10 | |||
| CN1289368A | 2001-03-28 | |||
| EP2374873A1 | 2011-10-12 |
Other References:
JUDITH BECKER, OSKAR ZELDER, STEFAN HäFNER, HARTWIG SCHRöDER, CHRISTOPH WITTMANN: "From zero to hero—Design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production", METABOLIC ENGINEERING, ACADEMIC PRESS, AMSTERDAM, NL, vol. 13, no. 2, 15 January 2011 (2011-01-15), AMSTERDAM, NL, pages 159 - 168, XP055523642, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2011.01.003
WANG YINGCHUN, LIU JIAO, NI XIAOMENG, LEI YU, ZHENG PING, DIAO AIPO: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 11, 25 November 2018 (2018-11-25), CN , pages 1760 - 1771, XP055901944, ISSN: 1000-3061, DOI: 10.13345/j.cjb.180041
"GenBank", Database accession no. FJ437239.1
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Manual of Methods for General Bacteriology", 1981, AMERICAN SOCIETY FOR BACTERIOLOGY
WANG Y C ET AL.: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 11, 2018, pages 1760 - 1771, XP055901944, DOI: 10.13345/j.cjb.180041
WANG YINGCHUN, LIU JIAO, NI XIAOMENG, LEI YU, ZHENG PING, DIAO AIPO: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 11, 25 November 2018 (2018-11-25), CN , pages 1760 - 1771, XP055901944, ISSN: 1000-3061, DOI: 10.13345/j.cjb.180041
"GenBank", Database accession no. FJ437239.1
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Manual of Methods for General Bacteriology", 1981, AMERICAN SOCIETY FOR BACTERIOLOGY
WANG Y C ET AL.: "Screening efficient constitutive promoters in Corynebacterium glutamicum based on time-series transcriptome analysis", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 11, 2018, pages 1760 - 1771, XP055901944, DOI: 10.13345/j.cjb.180041
Attorney, Agent or Firm:
BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)
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