Title:
ASPERGILLUS CRISTATUS SPORE POLYSACCHARIDE, AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/197466
Kind Code:
A1
Abstract:
An aspergillus cristatus spore polysaccharide, and a preparation method therefor and an application thereof. The preparation method comprises: performing wall-breaking on aspergillus cristatus spores, then performing drying, grinding into powder, uniformly mixing the powder with a deep eutectic solvent according to a material-liquid ratio of 1 g:20-50 mL, reacting for 30-55 min under the condition of 70°C, and then performing suction filtration to obtain a filtrate; adding the filtrate into an ethanol solution, standing for ethanol precipitation and then centrifuging, and collecting a precipitate; and dissolving the precipitate with distilled water, deproteinizing by using a Sevag method, dialyzing an obtained solution, and then performing vacuum freeze-drying to obtain the aspergillus cristatus spore polysaccharide. Test results show that the provided aspergillus cristatus spore polysaccharide has a good effect of improving metabolic syndrome, comprising: improving weight gain, fat accumulation, liver lipid accumulation and hepatic dysfunction, insulin resistance, dyslipidemia, and body inflammation caused by the metabolic syndrome, protecting a colonic barrier function, recovering the intestinal flora imbalance state caused by the metabolic syndrome, and adjusting the intestinal flora.
Inventors:
ZHU MINGZHI (CN)
WANG KUNBO (CN)
ZHOU FANG (CN)
LI QIN (CN)
ZHANG SHENG (CN)
FU DONGHE (CN)
LI JUAN (CN)
LIU ZHONGHUA (CN)
WANG KUNBO (CN)
ZHOU FANG (CN)
LI QIN (CN)
ZHANG SHENG (CN)
FU DONGHE (CN)
LI JUAN (CN)
LIU ZHONGHUA (CN)
Application Number:
PCT/CN2022/103531
Publication Date:
October 19, 2023
Filing Date:
July 04, 2022
Export Citation:
Assignee:
UNIV HUNAN AGRICULTURAL (CN)
International Classes:
C08B37/00; A23L33/125; A61K31/715; A61K36/06; A61P1/00; A61P1/16; A61P3/00; A61P3/06; A61P5/50; A61P29/00
Foreign References:
| CN111961142A | 2020-11-20 | |||
| CN113354754A | 2021-09-07 | |||
| CN109223834A | 2019-01-18 | |||
| CN107753527A | 2018-03-06 |
Other References:
XIE, ZHIYONG ET AL.: "Immunomodulatory Activity of Polysaccharides from the Mycelium of Aspergillus Cristatus, Isolated from Fuzhuan Brick Tea, Associated with the Regulation of Intestinal Barrier Function and Gut Microbiota", FOOD RESEARCH INTERNATIONAL, vol. 152, 16 December 2021 (2021-12-16), XP086964307, DOI: 10.1016/j.foodres.2021.110901
Attorney, Agent or Firm:
CHANGSHA QIHAO INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)
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