Title:
BACILLUS MUCILAGINOSUS AND HIGH-DENSITY FERMENTATION METHOD AND USE
Document Type and Number:
WIPO Patent Application WO/2016/004837
Kind Code:
A1
Abstract:
Provided are a wild Bacillus mucilaginosus mutation strain HSCUP-76-8 and a high-density fermentation method therefor, the deposit number of the mutation strain being CGMCC No. 8481. Using the mutation strain as a producing strain, a two-stage control high-density fermentation method is established. In the first stage, the fermentation parameters are controlled to lower the fermentation broth viscosity and promote strain growth, such that the volume of the strain reaches 2.0x109cfu/mL to 2.3x109cfu/mL. In the second stage, nutritional factors are restricted and fermentation conditions are adjusted so as to promote the forming of endospores, the production volume of the endospores being 1.5x109cfu/mL to 2.0x109cfu/mL. The fermentation period of the two-stage control high-density fermentation method is 32-48 hours.
Inventors:
MU YAOGUI (CN)
ZHAO LIANGQI (CN)
QIN WENWANG (CN)
LV LIHUA (CN)
CHEN QI (CN)
ZHAO LIANGQI (CN)
QIN WENWANG (CN)
LV LIHUA (CN)
CHEN QI (CN)
Application Number:
PCT/CN2015/083366
Publication Date:
January 14, 2016
Filing Date:
July 06, 2015
Export Citation:
Assignee:
SHANXI GREEN BIOTECHNOLOGY INDUSTRY CO LTD (CN)
International Classes:
C12N1/20; C12N3/00; C12R1/07
Foreign References:
| CN104130050A | 2014-11-05 | |||
| CN104928203A | 2015-09-23 | |||
| CN103525728A | 2014-01-22 | |||
| CN102260637A | 2011-11-30 | |||
| CN1504563A | 2004-06-16 |
Other References:
QIN, WENWANG ET AL.: "Screening and Breeding of a Bacillus Mucilaginosus Strain with Strong High Ability of Potassium Releasing", JOURNAL OF SHANXI AGRICULTURAL SCIENCES, vol. 43, no. 4, 18 April 2015 (2015-04-18), pages 434 - 438, ISSN: 1002-2481
XIAO, XIANGZHENG ET AL.: "UV Mutational Breeding of Bacillus Mucilaginosus HM8841", JOURNAL OF MICROBIOLOGY, vol. 26, no. 1, 30 January 2006 (2006-01-30), pages 36 - 39, ISSN: 1005-7021
HU , XIUFANG ET AL.: "Optimization of Fermentation Conditions of Bacillus Mucilaginosus Mutant 021120", CHINA BIOTECHNOLOGY, vol. 27, no. 9, 15 September 2007 (2007-09-15), pages 58 - 62, ISSN: 1671-8135
XIAO, XIANGZHENG ET AL.: "UV Mutational Breeding of Bacillus Mucilaginosus HM8841", JOURNAL OF MICROBIOLOGY, vol. 26, no. 1, 30 January 2006 (2006-01-30), pages 36 - 39, ISSN: 1005-7021
HU , XIUFANG ET AL.: "Optimization of Fermentation Conditions of Bacillus Mucilaginosus Mutant 021120", CHINA BIOTECHNOLOGY, vol. 27, no. 9, 15 September 2007 (2007-09-15), pages 58 - 62, ISSN: 1671-8135
Attorney, Agent or Firm:
LIFANG & PARTNERS LAW FIRM (CN)
北京市立方律师事务所 (CN)
北京市立方律师事务所 (CN)
Download PDF:
Previous Patent: CONSTANT-SPEED VARIABLE-PITCH VOITH-SCHNEIDER OMNI-DIRECTIONAL PROPELLER
Next Patent: VEHICLE DOOR ANTI-COLLISION DEVICE
Next Patent: VEHICLE DOOR ANTI-COLLISION DEVICE