Title:
BACILLUS SUBTILIS GENETICALLY ENGINEERED BACTERIUM FOR PRODUCING TAGATOSE AND METHOD FOR PREPARING TAGATOSE
Document Type and Number:
WIPO Patent Application WO/2022/148008
Kind Code:
A1
Abstract:
Provided are a bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.
Inventors:
MA YANHE (CN)
SHI TING (CN)
LI YUNJIE (CN)
HAN PINGPING (CN)
LI YUAN (CN)
SHI TING (CN)
LI YUNJIE (CN)
HAN PINGPING (CN)
LI YUAN (CN)
Application Number:
PCT/CN2021/108983
Publication Date:
July 14, 2022
Filing Date:
July 28, 2021
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N1/21; C12N11/14; C12N15/75; C12P19/02; C12R1/125
Domestic Patent References:
| WO2018182344A1 | 2018-10-04 | |||
| WO2015016544A1 | 2015-02-05 | |||
| WO2018004310A1 | 2018-01-04 |
Foreign References:
| CN112342179A | 2021-02-09 | |||
| CN107988286A | 2018-05-04 | |||
| CN109666620A | 2019-04-23 | |||
| CN103451141A | 2013-12-18 | |||
| CN105899660A | 2016-08-24 | |||
| CN106399427A | 2017-02-15 | |||
| CN105431541A | 2016-03-23 | |||
| US20160186162A1 | 2016-06-30 | |||
| CN105431541A | 2016-03-23 | |||
| CN109415715A | 2019-03-01 | |||
| CN109790524A | 2019-05-21 | |||
| CN106399427A | 2017-02-15 | |||
| CN107988286A | 2018-05-04 |
Other References:
CHEON JI-NA, KIM, SEONG-BO; PARK, SEONG-WON; HAN, JONG-KWON; KIM, PIL: "Comparative Analysis of Tagatose Productivity of Immobilized L-Arabinose Isomerase Expressed in Escherichia coli and Bacillus subtilis", FOOD SCIENCE AND BIOTECHNOLOGY, THE KOREA SOC. OF FOOD SCIENCE AND TECHNOLOGY, HEIDELBERG, vol. 17, no. 3, 30 June 2008 (2008-06-30), Heidelberg , pages 655 - 658, XP055949166, ISSN: 1226-7708
JIANG, ZHENGQIANG; YANG, SHAOQING: "Bacillus subtilis Expression System", PRINCIPLES OF ENZYMOLOGY AND ENZYME ENGINEERING IN FOOD, 30 September 2018 (2018-09-30), China, pages 39 - 42, XP009538062, ISBN: 978-7-5184-1952-4
"Oh D-K: Tagatose: properties, applications, and biotechnological processes", APP. MICROBIOL. BIOTECHNOL., vol. 76, 2007, pages 1 - 8
RHIMI MAGHAJARI NJUY MCHOUAYEKH HMAGUIN EHASER RBEJAR S: "Rational design of Bacillus stearothermophilus US 1001-arabinose isomerase: Potential applications for d-tagatose production", BIOCHIM, vol. 91, 2009, pages 650 - 653, XP026040354, DOI: 10.1016/j.biochi.2009.01.014
OH H-JKIM H-JOH D-K: "Increase in d-tagatose production rate by site-directed mutagenesis of 1-arabinose isomerase from Geobacillus thermodenitrificans", BIOTECHNOL. LETT., vol. 28, 2006, pages 145 - 149, XP019231191, DOI: 10.1007/s10529-005-5327-4
BOSSHART AHEE CSBECHTOLD MSCHIRMER TPANKE S: "Directed divergent evolution of a thermostable D-tagatose epimerase towards improved activity for two hexose substrates", CHEMBIOCHEM, vol. 16, 2015, pages 592 - 601, XP002789444
OH DKHONG SHLEE SH, ALDOLASE, ALDOLASE MUTANTS AND TAGATOSE USING THE SAME PRODUCTION METHODS AND COMPOSITIONS FOR PRODUCTION
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.", APPL. ENVIRON. MICROBIOL.,, vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
ZHANG, X. Z.ZHANG, Y. H. P.: "Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis.", MICROB. BIOTECHNOL., vol. 4, no. 1, 2011, pages 98 - 105, XP055701512, DOI: 10.1111/j.1751-7915.2010.00230.x
"NCBI-ProteinID", Database accession no. NP _388186
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis", APPL. ENVIRON. MICROBIOL., vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis", APPL. ENVIRON. MICROBIOL.,, vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
JIANG, ZHENGQIANG; YANG, SHAOQING: "Bacillus subtilis Expression System", PRINCIPLES OF ENZYMOLOGY AND ENZYME ENGINEERING IN FOOD, 30 September 2018 (2018-09-30), China, pages 39 - 42, XP009538062, ISBN: 978-7-5184-1952-4
"Oh D-K: Tagatose: properties, applications, and biotechnological processes", APP. MICROBIOL. BIOTECHNOL., vol. 76, 2007, pages 1 - 8
RHIMI MAGHAJARI NJUY MCHOUAYEKH HMAGUIN EHASER RBEJAR S: "Rational design of Bacillus stearothermophilus US 1001-arabinose isomerase: Potential applications for d-tagatose production", BIOCHIM, vol. 91, 2009, pages 650 - 653, XP026040354, DOI: 10.1016/j.biochi.2009.01.014
OH H-JKIM H-JOH D-K: "Increase in d-tagatose production rate by site-directed mutagenesis of 1-arabinose isomerase from Geobacillus thermodenitrificans", BIOTECHNOL. LETT., vol. 28, 2006, pages 145 - 149, XP019231191, DOI: 10.1007/s10529-005-5327-4
BOSSHART AHEE CSBECHTOLD MSCHIRMER TPANKE S: "Directed divergent evolution of a thermostable D-tagatose epimerase towards improved activity for two hexose substrates", CHEMBIOCHEM, vol. 16, 2015, pages 592 - 601, XP002789444
OH DKHONG SHLEE SH, ALDOLASE, ALDOLASE MUTANTS AND TAGATOSE USING THE SAME PRODUCTION METHODS AND COMPOSITIONS FOR PRODUCTION
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.", APPL. ENVIRON. MICROBIOL.,, vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
ZHANG, X. Z.ZHANG, Y. H. P.: "Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis.", MICROB. BIOTECHNOL., vol. 4, no. 1, 2011, pages 98 - 105, XP055701512, DOI: 10.1111/j.1751-7915.2010.00230.x
"NCBI-ProteinID", Database accession no. NP _388186
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis", APPL. ENVIRON. MICROBIOL., vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
YOU, C.ZHANG, X. Z.ZHANG, Y. H.: "Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis", APPL. ENVIRON. MICROBIOL.,, vol. 78, no. 5, 2012, pages 1593 - 1595, XP055378419, DOI: 10.1128/AEM.07105-11
Attorney, Agent or Firm:
INTELIGHT IP LAW FIRM (CN)
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