Title:
BACILLUS VELEZENSIS BRED BY ULTRASOUND-ASSISTED ADAPTIVE EVOLUTION, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/015805
Kind Code:
A1
Abstract:
A Bacillus velezensis bred by ultrasound-assisted adaptive evolution, and an application thereof. The Bacillus velezensis comprises a Bacillus velezensis stain US-ALE-BV3 with a preservation number of CGMCC No. 22846 and a Bacillus velezensis stain US-ALE-BV22 with a preservation number of CGMCC No. 22847. By using a Bacillus velezensis stain (B. velezensis CICC 23571) as an original strain, using an ultrasound-assisted adaptive evolution method, and continuously reducing the proportion of organic nitrogen in a culture medium and increasing the proportions of inorganic nitrogen and a sugar source, a Bacillus velezensis mutant strain with high production of polypeptides is obtained, so that the production of polypeptides is greatly increased.
Inventors:
RUAN SIYU (CN)
MA HAILE (CN)
LI YUNLIANG (CN)
JI YAOYONG (CN)
WANG XIAOJING (CN)
ZHOU ANQI (CN)
XIE PENGFEI (CN)
LIU XIAOSHUANG (CN)
MA HAILE (CN)
LI YUNLIANG (CN)
JI YAOYONG (CN)
WANG XIAOJING (CN)
ZHOU ANQI (CN)
XIE PENGFEI (CN)
LIU XIAOSHUANG (CN)
Application Number:
PCT/CN2021/138824
Publication Date:
February 16, 2023
Filing Date:
December 16, 2021
Export Citation:
Assignee:
UNIV JIANGSU (CN)
International Classes:
C12N1/20; A23K10/18; A23L29/00; C12N13/00; C12R1/07
Foreign References:
| CN113201476A | 2021-08-03 | |||
| CN111471635A | 2020-07-31 | |||
| CN110643648A | 2020-01-03 | |||
| CN110591938A | 2019-12-20 | |||
| US20100248307A1 | 2010-09-30 |
Other References:
ZHAO, YUNFEI ET AL.: "Improve Glutamate Decarboxylase Activity from Bacillus megaterium by Directed Evolution", FOOD SCIENCE, vol. 39, no. 18, 31 December 2018 (2018-12-31), pages 179 - 185, XP055977118, ISSN: 1002-6630
BAO HAIJIAO; LIU RONGMING; LIANG LIYA; JIANG YOUMING; JIANG MIN; MA JIANGFENG; CHEN KEQUAN; JIA HONGHUA; WEI PING; OUYANG PINGKAI: "Succinic acid production from hemicellulose hydrolysate by anEscherichia colimutant obtained by atmospheric and room temperature plasma and adaptive evolution", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 66, 18 July 2014 (2014-07-18), US , pages 10 - 15, XP029064381, ISSN: 0141-0229, DOI: 10.1016/j.enzmictec.2014.04.017
BAO HAIJIAO; LIU RONGMING; LIANG LIYA; JIANG YOUMING; JIANG MIN; MA JIANGFENG; CHEN KEQUAN; JIA HONGHUA; WEI PING; OUYANG PINGKAI: "Succinic acid production from hemicellulose hydrolysate by anEscherichia colimutant obtained by atmospheric and room temperature plasma and adaptive evolution", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 66, 18 July 2014 (2014-07-18), US , pages 10 - 15, XP029064381, ISSN: 0141-0229, DOI: 10.1016/j.enzmictec.2014.04.017
Attorney, Agent or Firm:
NANJING DOYES INTELLECTUAL PROPERTY CO., LTD. (CN)
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