Title:
BACTERIAL CONTINUOUS EVOLUTION SYSTEM, ORTHOGONAL ERROR-PRONE DNA POLYMERASE, AND CONTINUOUS EVOLUTION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/041031
Kind Code:
A1
Abstract:
The present invention relates to a bacterial continuous evolution system, an orthogonal error-prone DNA polymerase, and a continuous evolution method. According to the present invention, after an orthogonal DNA replication system is combined with an orthogonal error-prone DNA polymerase, what is obtained is a continuous evolution method that is capable of comprising all mutation types and realizing long DNA fragment mutation and features high continuity and simple and convenient operation. By means of inducing opening and closing of DNA polymerase expression, switching between a linear plasmid error-prone mutation process and a high-fidelity replication process is achieved, thereby achieving the efficient continuous evaluation of a target DNA sequence.
Inventors:
LIU YANFENG (CN)
CHEN JIAN (CN)
DU GUOCHENG (CN)
LIU LONG (CN)
LV XUEQIN (CN)
TIAN RONGZHEN (CN)
ZHAO RUNZHI (CN)
CHEN JIAN (CN)
DU GUOCHENG (CN)
LIU LONG (CN)
LV XUEQIN (CN)
TIAN RONGZHEN (CN)
ZHAO RUNZHI (CN)
Application Number:
PCT/CN2023/092743
Publication Date:
February 29, 2024
Filing Date:
May 08, 2023
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N15/75; C12N1/21; C12N9/12; C12N15/54; C12R1/07
Domestic Patent References:
| WO2019079775A2 | 2019-04-25 |
Foreign References:
| CN115772533A | 2023-03-10 | |||
| US20220195442A1 | 2022-06-23 | |||
| CN113801888A | 2021-12-17 |
Other References:
DATABASE UniprotKB 5 July 2004 (2004-07-05), ANONYMOUS : "Q6X3W4_BP35C Protein DNA-directed DNA polymerase", XP093142176, retrieved from Uniprot Database accession no. Q6X3W4
ARZUMANYAN GARRI A., GABRIEL KRISTIN N., RAVIKUMAR ARJUN, JAVANPOUR ALEX A., LIU CHANG C.: "Mutually Orthogonal DNA Replication Systems In Vivo", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 7, no. 7, 20 July 2018 (2018-07-20), Washington DC ,USA , pages 1722 - 1729, XP093142177, ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00195
ARJUN RAVIKUMAR, ADRIAN ARRIETA, CHANG C LIU: "An orthogonal DNA replication system in yeast", NATURE CHEMICAL BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 10, no. 3, 1 March 2014 (2014-03-01), New York, pages 175 - 177, XP055618678, ISSN: 1552-4450, DOI: 10.1038/nchembio.1439
ARZUMANYAN GARRI A., GABRIEL KRISTIN N., RAVIKUMAR ARJUN, JAVANPOUR ALEX A., LIU CHANG C.: "Mutually Orthogonal DNA Replication Systems In Vivo", ACS SYNTHETIC BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON DC ,USA, vol. 7, no. 7, 20 July 2018 (2018-07-20), Washington DC ,USA , pages 1722 - 1729, XP093142177, ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00195
ARJUN RAVIKUMAR, ADRIAN ARRIETA, CHANG C LIU: "An orthogonal DNA replication system in yeast", NATURE CHEMICAL BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 10, no. 3, 1 March 2014 (2014-03-01), New York, pages 175 - 177, XP055618678, ISSN: 1552-4450, DOI: 10.1038/nchembio.1439
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE et al. (CN)
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