Title:
BASE-PROTECTED OLIGONUCLEOTIDE
Document Type and Number:
WIPO Patent Application WO/2013/122236
Kind Code:
A1
Abstract:
Provided is a protected nucleotide for elongation that makes it possible to realize a method for producing oligonucleotides by the phosphoramidite process that allows purification to be conducted by liquid-liquid extraction efficiently and at high yield. It was discovered that this can be achieved by using a specific base-protected oligonucleotide and/or a specific aromatic protected oligonucleotide containing a 3'-position branched chain.
Inventors:
HIRAI KUNIHIRO (JP)
KATAYAMA SATOSHI (JP)
TORII TAKAYOSHI (JP)
NAKAYA RYOTARO (JP)
TAKAHASHI DAISUKE (JP)
KATAYAMA SATOSHI (JP)
TORII TAKAYOSHI (JP)
NAKAYA RYOTARO (JP)
TAKAHASHI DAISUKE (JP)
Application Number:
PCT/JP2013/053769
Publication Date:
August 22, 2013
Filing Date:
February 15, 2013
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C07H19/073; C07H21/04
Domestic Patent References:
| WO2005070859A1 | 2005-08-04 |
Foreign References:
| JPH09500398A | 1997-01-14 | |||
| JP2010275254A | 2010-12-09 | |||
| JP2010275254A | 2010-12-09 | |||
| JP2012033429A | 2012-02-16 | |||
| JP2012254718A | 2012-12-27 |
Other References:
S.YU.ANDREEV ET AL.: "Synthesis of Oligodeoxyribonucleotides Containing Oleylamine Moieties", RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, vol. 27, no. 3, 2001, pages 184 - 190, XP055159958
T.S.ORETSKAYA ET AL.: "A novel method of introducing hydrophobic moieties into ologonucleotides for covalent and non-covalent immobilization on electrode surfaces", BIOELECTROCHEMISTRY, vol. 56, no. 1-2, 2002, pages 47 - 51, XP055159959
S. L. BEAUCAGE; D. E. BERGSTORM; G. D. GLICK; R. A. JONES: "Current Protocols in Nucleic Acid Chemistry", 2000, JOHN WILEY & SONS
NUCLEIC ACID RES., vol. 18, no. 11, 1990, pages 3155 - 3159
J. ORG. CHEM., vol. 71, no. 20, 2006, pages 7907 - 7910
"Greene's PROTECTIVE GROUPS IN ORGANIC SYNTHESIS", 2006, JOHN WILLY&SONS
GREENE'S: "PROTECTIVE GROUPS IN ORGANIC SYNTHESIS", 2006, JOHN WILLY & SONS
ORGANIC LETTERS, vol. 7, no. 24, 2005, pages 5389 - 5392
JOURNAL OF AMERICAN CHEMICAL SOCIETY, vol. 104, 1982, pages 1316 - 1319
M.H. CARUTHERS ET AL., METHOD IN ENZYMOLOGY, vol. 154, 1987, pages 287 - 313
S. L. BEAUCAGE; M. H. CARUTHERS, TETRAHEDRON LETTERS, vol. 22, 1981, pages 1859 - 1862
AUST. J. CHEM., vol. 63, 2010, pages 227 - 235
S.L. BEAUCAGE; M.H. CARUTHERS, TETRAHEDRON LETTERS, vol. 22, 1981, pages 1859 - 1862
GREENE'S: "PROTECTIVE GROUPS IN ORGANIC SYNTHESIS", 2006, JOHN WILLY&SONS
RICHARD T. PON ET AL., NUCLEIC ACIDS RESEARCH, vol. 32, 2004, pages 623 - 631
See also references of EP 2816053A4
T.S.ORETSKAYA ET AL.: "A novel method of introducing hydrophobic moieties into ologonucleotides for covalent and non-covalent immobilization on electrode surfaces", BIOELECTROCHEMISTRY, vol. 56, no. 1-2, 2002, pages 47 - 51, XP055159959
S. L. BEAUCAGE; D. E. BERGSTORM; G. D. GLICK; R. A. JONES: "Current Protocols in Nucleic Acid Chemistry", 2000, JOHN WILEY & SONS
NUCLEIC ACID RES., vol. 18, no. 11, 1990, pages 3155 - 3159
J. ORG. CHEM., vol. 71, no. 20, 2006, pages 7907 - 7910
"Greene's PROTECTIVE GROUPS IN ORGANIC SYNTHESIS", 2006, JOHN WILLY&SONS
GREENE'S: "PROTECTIVE GROUPS IN ORGANIC SYNTHESIS", 2006, JOHN WILLY & SONS
ORGANIC LETTERS, vol. 7, no. 24, 2005, pages 5389 - 5392
JOURNAL OF AMERICAN CHEMICAL SOCIETY, vol. 104, 1982, pages 1316 - 1319
M.H. CARUTHERS ET AL., METHOD IN ENZYMOLOGY, vol. 154, 1987, pages 287 - 313
S. L. BEAUCAGE; M. H. CARUTHERS, TETRAHEDRON LETTERS, vol. 22, 1981, pages 1859 - 1862
AUST. J. CHEM., vol. 63, 2010, pages 227 - 235
S.L. BEAUCAGE; M.H. CARUTHERS, TETRAHEDRON LETTERS, vol. 22, 1981, pages 1859 - 1862
GREENE'S: "PROTECTIVE GROUPS IN ORGANIC SYNTHESIS", 2006, JOHN WILLY&SONS
RICHARD T. PON ET AL., NUCLEIC ACIDS RESEARCH, vol. 32, 2004, pages 623 - 631
See also references of EP 2816053A4
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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