BEAD SEALING METHOD, METHOD FOR DETECTING TARGET MOLECULE, ARRAY, KIT, AND TARGET MOLECULE DETECTION DEVICE

Title:

BEAD SEALING METHOD, METHOD FOR DETECTING TARGET MOLECULE, ARRAY, KIT, AND TARGET MOLECULE DETECTION DEVICE

Document Type and Number:

WIPO Patent Application WO/2012/121310

Kind Code:

A1

Abstract:

A technique allowing low-concentration target molecules to be detected with high sensitivity is provided. The present invention includes: a bead introduction step for introducing a hydrophilic solvent (42) that contains beads (40), (41') into a space (30) between a lower-layer section (10) and an upper-layer section (20), the lower-layer section having a plurality of receptacles (13) capable of receiving only one of the beads (41), (41'), the receptacles being formed at a distance from each other by side walls (12) having hydrophobic upper surfaces, and the upper-layer section (20) facing that surface of the lower-layer section (10) in which the receptacles (13) are formed; and a hydrophobic solvent introduction step for introducing a hydrophobic solvent (43) into the space (30) following the bead introduction step.

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Inventors:

NOJI HIROYUKI
IINO RYOTA
ARAKI SUGURU

Application Number:

PCT/JP2012/055884

Publication Date:

September 13, 2012

Filing Date:

March 07, 2012

Export Citation:

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Assignee:

JAPAN SCIENCE & TECH AGENCY (JP)
NOJI HIROYUKI
IINO RYOTA
ARAKI SUGURU

International Classes:

G01N35/02; B01J19/00; G01N21/64; G01N33/53; G01N37/00

Foreign References:

JP2009109213A	2009-05-21
JP2009162549A	2009-07-23
JP2004309405A	2004-11-04
JP2008111798A	2008-05-15
JP2004309405A	2004-11-04

Other References:

DAVID M RISSIN ET AL.: "Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations", NATURE BIOTECHNOLOGY LETTERS, vol. 28, no. 6, June 2010 (2010-06-01), pages 595 - 599, XP002662812, DOI: doi:10.1038/NBT.1641
S. SAKAKIHARA ET AL., LAB CHIP, vol. 10, 2010, pages 3355 - 3362
DAVID M RISSIN ET AL., NATURE BIOTECHNOLOGY
See also references of EP 2685266A4

Attorney, Agent or Firm:

HARAKENZO WORLD PATENT & TRADEMARK (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)

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