BENZENE, PYRIDINE, NAPHTALENE OR BENZOPHENONE DERIVATIVES AS INHIBITORS OF SQUALENE SYNTHETASE AND PROTEIN FARNESYLTRANSFERASE

This is a continuation-in-part of U.S. patent application Serial No.

08/429,095, filed May 3, 1995, which is a continuation-in-part of U.S. patent application Serial No. 322,783, filed October 18, 1994, which is a continuation- in-part of U.S. patent application Serial No. 289,711 , filed August 12, 1994, which is a continuation-in-part of U.S. patent application Serial No. 147,708, filed November 4, 1993.

Technical Field

The present invention relates to new cyclobutane dicarboxylic acid compounds which are useful in inhibiting de novo squalene production or inhibiting protein farnesyltransferase and the famesylation of the oncogene protein Ras or inhibiting fungal growth and to chemotherapeutic, antifungal, hypolipidaemic and antiatherosclerotic compositions containing such compounds and to a method of using such compounds for inhibiting cholesterol biosynthesis and atherosclerosis, for inhibiting protein famesyl-transferase and the famesylation of the oncogene protein Ras and as antifungals.

Background of the Invention

Squalene synthetase is a microsomal enzyme which catalyzes the reductive dimerization of two molcules of famesyl pyrophosphate (FPP) in the presence of nicotinamide adenine dinucleotide phosphate, reduced form, (NADPH) to form squalene (Poulter, C. D., Rilling, H. C, in "Biosynthesis of Isoprenoid Compounds", Vol. I, Chapter 8, pp. 413-441 , J. Wiley and Sons, 1981 and references therein). This enzyme is the first committed step of the de novo cholesterol biosynthetic pathway. Thus inhibition of squalene synthetase will lead to inhibition of cholesterol biosynthesis and thus will act as a hypocholesterolemic. Thus squalene synthetase inhibitors ultimately should be useful for the treatment and prevention of hyperlipidaemia or atherosclerosis or other disorders resulting from an excess of cholesterol.

Disclosure of the Invention

In accordance with the present invention there are provided compounds of the formula (I), (II), (III) and (IV):

wherein

A1 , A2, A3, A4, A5 and AQ are independently selected from the group consisting of

(1) hydrogen; (2) halogen;

(3) loweralkyl;

(4) hydroxy;

(5) alkoxy;

(6) -X-T-G wherein at each occurrence T is independently selected from the group consisting of a) a covalent bond, b) -C(O)-, c) -C(S)- and d) -S(0) ₂ -,

at each occurrence X is independently selected from the group consisting of a) a covalent bond, b) -CH ₂ -, c) -0-, d) -S- and e) -N(R _a )- wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl,

and at each occurrence G is independently selected from the group consisting of a) R ₂ , b) -N(R ₁ )(R ₂ ) wherein at each occurrence R ₁ is independently selected from the group consisting of (i) -CH(R _d )C(0)OR _e wherein at each occurrence R _d is independently selected from the group consisting of loweralkyl, cycloalkyl, cycloalkylalkyl, alkoxyalkyl, thioalkoxyalkyl, hydroxyalkyl, aminoalkyl, carboxyalkyl, alkoxycarbonylalkyl, arylalkyl and alkylsulfonylalkyl and at each occurrence R _e is independently selected from the group consisting of hydrogen and carboxy-protecting group,

(ii) aryl,

(iii) arylalkyl,

(iv) heterocyclic, (v) (heterocyclic)alkyl,

(vi) cycloalkylalkyl and

(vii) aryl, heterocyclic, arylalkyl or (heterocyclic)alkyl wherein the aryl group, the aryl part of the arylalkyl group, the heterocyclic group or the heterocyclic part of the (heterocyclic)alkyl group is substituted with one or two substituents -W-R ₄ wherein at each occurrence W is independently selected from the group consisting of (a) a covalent bond, (b) -C(O)-, (c) -CH ₂ -, (d) -0-, (e) -S(0) _p -

wherein p is 0, 1 or 2, (f) -N(R _C )- wherein R _c is hydrogen or loweralkyl, (g) -CH ₂ 0-, (h) -CH ₂ S(0) _p - wherein p is 0, 1 or 2 and (i) -CH ₂ N(R _C )- wherein R _c is hydrogen or loweralkyl and at each occurrence R ₄ is independently selected from the group consisting of (a) aryl, (b) arylalkyl, (c) cycloalkyl, (d) cycloalkylalkyl, (e) heterocyclic and (f) (heterocyclic)alkyl,

and

at each occurrence R2 is independently selected from the group consisting of

(i) aryl, (ii) arylalkyl,

(iii) alkenyl,

(iv) alkynyl,

(v) arylalkenyl,

(vi) arylalkynyl, (vii) (heterocyclic)alkyl,

(viii) aryloxyalkyl,

(ix) aryloxyalkenyl,

(x) arylalkoxyalkenyl,

(xi) arylalkyl wherein the alkyl group is substituted with (a) -OR10 wherein R10 is hydrogen or alkanoyl or (b) -C(0)OR _h wherein R _h is hydrogen or a carboxy-protecting group,

(xii) aroyloxyalkyl, and

(xiii) aryl, arylalkyl or (heterocyclic)alkyl wherein the aryl group, the the aryl part of the arylalkyl group or the heterocyclic part of the (heterocyclic)alkyl group is substituted with one or two substituents -Y-R ₃ wherein at each occurrence Y is independently selected from the group consisting of (a) a covalent bond, (b) -C(O)-,

(c) -CH ₂ -, (d) -0-, (e) -S(0) _m - wherein m is 0, 1 or 2,

(f) -N(R _b )- wherein R _b is hydrogen or loweralkyl, (g) -CH ₂ 0-, (h) -CH ₂ S(0) _m - wherein m is 0, 1 or 2 and (i) -CH ₂ N(R _b )- wherein R _b is hydrogen or loweralkyl and at each occurrence R ₃ is independently selected from the group consisting of (a) aryl, (b) arylalkyl, (c) cycloalkyl,

(d) cycloalkylalkyl, (e) heterocyclic and (f) (heterocyclic)alkyl,

and

c) -NHR _2a or -OR _2a wherein at each occurrence R _2a is independently selected from the group consisting of (i) arylalkyl and

(ii) heterocyclicalkyl, wherein the alkyl part of the arylalkyl group or the heterocyclicalkyl group is substituted with an arylalkyl group and wherein the aryl part of the arylalkyl group or the heterocyclic part of the heterocyclicalkyl group is substituted with one or two substituents -Y'-R ₃ * wherein at each occurrence Y' is independently selected from the group consisting of (a) a covalent bond, (b) -C(O)-, (c)-CH ₂ -, (d) -0-, (e) -S(0) _m .- wherein m' is 0, 1 or 2, (f) -N(R _b >)- wherein R _b . is hydrogen or loweralkyl, (g) -CH ₂ 0-, (h) -CH ₂ S(0) _m ,- wherein m ¹ is 0, 1 or 2 and (i) -CH ₂ N(R _b .)- wherein R _b . is hydrogen or loweralkyl and at each occurrence R ₃ . is independently selected from the group consisting of (a) aryl, (b) arylalkyl, (c) cycloalkyl, (d) cycloalkylalkyl, (e) heterocyclic and (f) (heterocyclic)alkyl;

(7) -Z wherein at each occurrence Z is independently selected from the group consisting of

a) -Q-D wherein at each occurrence D is independently selected from the group consisting of

(i) -C(0)R ₆ wherein at each occurrence R ₆ is independently selected from the group consisting of hydrogen and a carboxy-protecting group,

(ii) -C(0)H, (iii) -CH ₂ OH, (iv) -C(0)CF ₃ , (v) -CH(OH)CF ₃ ,

(vi) -C(0H)(CF ₃ ) ₂ , (vii) -C(0)NH ₂ , (viii) -C(0)NHOH,

(ix) -CH(=NOH), (x) -S(0) ₂ NH ₂ ,

(xi) -NHS(0) ₂ CH ₃ or -NHS(0) ₂ CF ₃ ,

(xii) 5-tetrazolyl,

(xiv) R» wherein R3 ₀ is -CN, -N02, or -C0 ₂ R ₃ *ι wherein R ₃ ι is hydrogen, aryl or loweralkyl,

(xv) wherein at each occurrence R ₃₂ is independently selected from the group consisting of hydrogen and loweralkyl,

(xvi) wherein at each occurrence R ₃₃ is independently selected from the group consisting of hydrogen and loweralkyl,

(xvii) wherein at each occurrence R ₃ 4 is independently selected from the group consisting of hydrogen, loweralkyl, alkenyl, alkoxyalkyl and benzyl,

JC>

(xviii)

(xix)

(xxi)

(xxv)

and wherein at each occurrence Q is independently selected from the group consisting of (i) a covalent bond, (ii) -OCH ₂ -, (iii) alkylene, and (iv) alkenylene;

and c)

-C(0)OR _f

wherein at each occurrence R _f is independently selected from the group consisting of hydrogen and a carboxy-protecting group;

(8) -C(0)R _2a wherein at each occurrence R _2a is independently defined as above;

(9) -CH(OH)R _2a wherein at each occurrence R _2a is independently defined as above;

(10) -CH=C(R _2b )(R _2c ) wherein at each occurrence R _2b is independently selected from arylalkyl and at each occurrence R _2c is independently selected from the group consisting of aryl and heterocyclic wherein the aryl or heterocyclic ring is subsubstituted with -Y'-R ₃ * wherein at each occurrence Y' and R ₃ . are independently defined as above,

(11) -C(0)-CH(R _2a )CH(R _2d )C(0)OR _g wherein at each occurrence R _2a is independently defined as above, at each occurrence R ₂ is independently selected from aryl and at each occurrence R _g is independently selected from the group consisting of hydrogen and a carboxy-protecting group;

and

(12) -C(0)NH(aιylalkyl);

or any two adjacent substituents selected from A ₁ , A ₂ , A ₃ , A ₄ , A ₅ and A ₆ taken together form a 5-, 6- or 7-membered cyclic anhydride group;

with the proviso that one or two of Λ-j, A2, A3, A4, A5 and A6 is independently selected from the group consisting of

(1) -X-T-G wherein at each occurrence X, T and G are independently defined as above,

(2) -C(0)R _2a wherein at each occurrence R _2a is independently defined as above,

(3) -CH(OH)R _2a wherein at each occurrence R _2a is independently defined as above, (4) -CH=C(R _2b )(R _2c ) wherein at each occurrence R _2b and R _2c are independently defined as above, and

(5) -C(0)-CH(R _2a )CH(R _2d )C(0)OR _g wherein at each occurrence R _2a , R _2d and

R _g are independently defined as above,

and with the proviso that one, two or three of Ai , A2, A ₃ , A4, A5 and AQ is -Z which at each occurrence is independently defined as above;

or a pharmaceutically acceptable salt thereof.

Preferred compounds of the invention are compounds of formula (I), (II), (III) or (IV) wherein A-j, A2, A3, A4, A5 and Aβ are independently selected from

(1) hydrogen,

(2) halogen, (3) loweralkyl;

(4) hydroxy;

(5) alkoxy;

(6) -C(0)NR-|R2 , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein at each occurrence R1 is independently selected from the group consisting of (i) aryl, (ii) arylalkyl, (iii) heterocyclic,

(iv) (heterocyclic)alkyl and (vi) R ₂ , and at each occurrence R ₂ is independently elected from the group consisting of (i) aryl, (ii) arylalkyl, (iii) alkenyl, (iv) alkynyl, (v) arylalkenyl, (vi) arylalkynyl, (vii) (heterocyclic)alkyl, (viii) aryloxyalkyi, (ix) aryloxyalkenyl, (x) arylalkoxyalkenyl, (xi) arylalkyl wherein the alkyl group is substituted with -OR10 wherein R10 is hydrogen or alkanoyl, and (xii) aryl, arylalkyl or (heterocyclic)alkyl wherein the aryl group, the the aryl part of the arylalkyl group or the heterocyclic part of the (heterocyclic)alkyl group

is substituted with -Y-R ₃ wherein at each occurrence Y is independently selected from (a) a covalent bond, (b) -C(O)-, (c) -CH ₂ -, (d) -0-, (e) -S(0) _m - wherein m is 0, 1 or 2, (f) -N(R _b )- wherein R _b is hydrogen or loweralkyl, (g) -CH ₂ 0-, (h) -CH ₂ S(0) _m - wherein m is 0, 1 or 2 and (i) -CH ₂ N(R _b )- wherein R _b is hydrogen or loweralkyl and at each occurrence R ₃ is independently selected from (a) aryl, (b) arylalkyl, (c) cycloalkyl, (d) cycloalkylalkyl, (e) heterocyclic and (f) (heterocyclic)alkyl; and

(7) -Q-C(0)R ₆ wherein at each occurrence Q is independently selected from (a) a covalent bond, (b) alkylene, and (c) alkenylene and R ₆ is -OR ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; with the proviso that one or two of Ai , A2, A _3l A4. A5 and AQ is -C(0)NR- _| R ₂ , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein R ₁ and R ₂ are as defined above, and with the proviso that one, two or three of A1 , A2, A3, A4, A ₅ and AQ is -Q-C(0)R ₆ wherein Q and Rβ are as defined above.

More preferred compounds of the invention are compounds of formula (I) wherein A-i, A2, A3, A4, A5 and Aβ are independently selected from

(1) hydrogen,

(2) halogen,

(3) loweralkyl;

(4) hydroxy; (5) alkoxy;

(6) -C(0)NR ₁ R ₂ , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(O)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein at each occurrence R-) is independently selected from the group consisting of (i) aryl, (ii) arylalkyl, (iii) heterocyclic, (iv) (heterocyclic)alkyl and (vi) R ₂ , and at each occurrence R ₂ is independently selected from the group consisting of (i) aryl, (ii) arylalkyl, (iii) alkenyl, (iv) alkynyl, (v) arylalkenyl, (vi) arylalkynyl, (vii) (heterocyclic)alkyl,

(viii) aryloxyalkyi, (ix) aryloxyalkenyl, (x) arylalkoxyalkenyl, (xi) arylalkyl wherein the alkyl group is substituted with -OR10 wherein R10 is hydrogen or alkanoyl, and (xii) aryl, arylalkyl or (heterocyclic)alkyl wherein the aryl group, the the aryl part of the arylalkyl group or the heterocyclic part of the (heterocyclic)alkyl group is substituted with -Y-R3 wherein at each occurrence Y is independently selected from (a) a covalent bond, (b) -C(O)-, (c) -CH ₂ -, (d) -0-, (e) -S(0) _m - wherein m is 0, 1 or 2, (f) -N(R _b )- wherein R _b is hydrogen or loweralkyl, (g) -CH ₂ 0-, (h) -CH ₂ S(0) _m - wherein m is 0, 1 or 2 and (i) -CH ₂ N(R _b )- wherein R _b is hydrogen or loweralkyl and at each occurrence R ₃ is independently selected from (a) aryl, (b) arylalkyl, (c) cycloalkyl, (d) cycloalkylalkyl, (e) heterocyclic and (f) (heterocyclic)alkyl; and

(7) -Q-C(0)R ₆ wherein at each occurrence Q is independently selected from (a) a covalent bond, (b) alkylene, and (c) alkenylene and R ₆ is -0R ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; with the proviso that one or two of A-i, A ₂ , A3, A4, A5 and AQ is -C(0)NRιR ₂ , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or

-CH ₂ -C(0)NR ₁ R ₂ wherein R ₁ and R ₂ are as defined above, and with the proviso that one, two or three of A-i, A2, A3, A4, A5 and AQ is -Q-C(0)R ₆ wherein Q and RQ are as defined above.

Even more preferred compounds of the invention are compounds of formula (I) wherein A-j, A2. A3, A4, A5 and AQ are independently selected from

(1) hydrogen,

(2) halogen,

(3) loweralkyl;

(4) hydroxy; (5) alkoxy;

(6) -C(0)NRιR2 , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or

-CH ₂ -C(0)NR ₁ R ₂ wherein at each occurrence Ri is independently selected from (i) arylalkyl, (ii) (heterocyclic)alkyl and (iii) R ₂ , and at each occurrence R ₂ is independently selected from (i) arylalkyl, (ii) arylalkenyl, (iii) aryloxyalkyi, (iv) aryloxyalkenyl, (v) arylalkoxyalkenyl, and (vi) aryl, arylalkyl or (heterocyclic)alkyl wherein the aryl group, the the aryl part of the arylalkyl group or the heterocyclic part of the (heterocyclic)alkyl group is substituted with -Y-R3 wherein at each occurrence Y is independently selected from (a) a covalent bond, (b) -CH ₂ -, and (c) -O- and at each occurrence R ₃ is independently selected from

(a) aryl, (b) arylalkyl, (c) heterocyclic and (d) (heterocyclic)alkyl; and

(7) -C(0)R ₆ wherein R ₆ is -OR ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; with the proviso that one or two of A1 , A2, A3, A4, A5 and AQ is -C(0)NRι R2 , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein R ₁ and R ₂ are as defined above, and with the proviso that one, two or three of A-j, A2, A3, A4, A5 and AQ is -C(0)R ₆ wherein RQ is as defined above.

Even more highly preferred compounds of the invention are compounds of formula (I) wherein Aι, A2, A3, A4, A5 and AQ are independently selected from

(1) hydrogen,

(2) halogen,

(3) loweralkyl;

(4) hydroxy; (5) alkoxy;

(6) -C(0)NR ₁ R ₂ , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein at each occurrence R-j is independently selected from benzyl, 2- ethoxybenzyl, chlorobenzyl, dichlorobenzyl, phenethyl, 3-phenylpropyl, 4- phenylbutyl and

4-(phenoxy)benzyl, and at each occurrence R2 is independently selected from 4-(phenoxy)benzyl, 3-(4-methylphenoxy)benzyl, and 4-(phenoxy)phenethyl; and

(7) -C(0)R ₆ wherein R ₆ is -OR ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; with the proviso that one or two of Ai , A2, A3, A4, A5 and AQ is -C(0)NRι R2 , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR.,R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein R ₁ and R ₂ are as defined above, and with the proviso that one, two or three of A-j, A2, A3, A4, A5 and AQ is -C(0)R ₆ wherein RQ is as defined above.

Most preferred compounds of the invention are compounds of formula (I) wherein A1 is -C(0)NR-|R2 , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein R-j is selected from benzyl, chlorobenzyl, 2-ethoxybenzyl, dichlorobenzyl, phenethyl, 3-phenylpropyl, 4-phenylbutyl and 4-(phenoxy)benzyl, and R2 is selected from 4-(phenoxy)benzyl, 3-(4-methylphenoxy)benzyl, and 4-(phenoxy)phenethyl; A ₂ , A ₄ and A ₅ are -C(0)R ₆ wherein R ₆ is -0R ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; or A ₃ and A ₄ are -C(0)R ₆ wherein R ₆ is -OR ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; or A ₂ and A ₄ are -C(0)R ₆ wherein R ₆ is -0R ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy- protecting group; and the remaining members of the group A-i, A2, A3, A4, A5 and AQ are hydrogen.

Most preferred compounds of the invention also are compounds of formula (I) wherein A1 and A ₄ are -C(0)NR ₁ R ₂ , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR ₁ R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein at each occurrence R1 is independently selected from benzyl, chlorobenzyl, dichlorobenzyl, 2-ethoxybenzyl, phenethyl,

3-phenylpropyl, 4-phenylbutyl and 4-(phenoxy)benzyl, and at each occurrence R2 is independently selected from 4-(ρhenoxy)benzyl,

3-(4-methylphenoxy) benzyl, and 4-(phenoxy)phenethyl; and A ₂ and A ₅ are R ₆ is -OR ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy- protecting group; and the remaining members of the group Ai , A2, A3, A4, A5 and AQ are hydrogen;

or A., and A ₅ are -C(0)NRιR ₂ , -N(R _a )-C(0)NR ₁ R ₂ wherein R _a is hydrogen, loweralkyl, cycloalkyl, cycloalkylalkyl or arylalkyl, -0-C(0)NR.,R ₂ or -CH ₂ -C(0)NR ₁ R ₂ wherein at each occurrence R1 is independently selected from benzyl, chlorobenzyl, dichlorobenzyl, phenethyl, 3-phenylpropyl, 4- phenylbutyl and 4-(phenoxy)benzyl, and at each occurrence R ₂ is independently selected from 4-(phenoxy)benzyl and 4-(phenoxy)phenethyl; and A ₂ and A ₄ are R ₆ is -0R ₇ wherein at each occurrence R ₇ is independently hydrogen or a carboxy-protecting group; and the remaining members of the group A-i, A2. A3, A4. A5 and AQ are hydrogen.

The present invention also relates to processes for preparing the compounds of formula (I), (II), (III) and (IV) and to the synthetic intermediates useful in such processes.

In a further aspect of the present invention are disclosed pharmaceutical compositions which comprise a compound of the present invention in combination with a pharmaceutically acceptable carrier.

In yet another aspect of the present invention are disclosed pharmaceutical compositions which comprise a compound of the present invention in combination with another chemotherapeutic agent and a pharmaceutically acceptable carrier.

In yet another aspect of the present invention is disclosed a method for inhibiting protein famesyltransferase in a human or lower mammal, comprising administering to the patient a therapeutically effective amount of a compound of the invention. In yet another aspect of the present invention is disclosed a method for inhibiting or treating cancer in a human or lower mammal, comprising administering to the patient a therapeutically effective amount of a compound of the invention alone or in combination with another chemotherapeutic agent

In yet another aspect of the present invention is disclosed a method for treating or preventing restenosis in a human or lower mammal, comprising administering to the patient a therapeutically effective amount of a compound of the invention.

The compounds of the invention comprise asymmetrically substituted carbon atoms. As a result, all stereoisomers of the compounds of the invention are meant to be included in the invention, including racemic mixtures, mixtures of diastereomers, as well as single diastereomers of the compounds of the invention. The terms "S" and "R" configuration, as used herein, are as defined by the IUPAC 1974 Recommendations for Section E, Fundamental Stereochemistry, Pure Appl. Chem. (1976) 45, 13-30.

The term "carboxy protecting group" as used herein refers to a carboxylic acid protecting ester group employed to block or protect the carboxylic acid functionality while the reactions involving other functional sites of the compound are carried out. Carboxy protecting groups are disclosed in Greene, "Protective Groups in Organic Synthesis" pp. 152-186 (1981), which is hereby incorporated herein by reference. In addition, a carboxy protecting group can be used as a prodrug whereby the carboxy protecting group can be readily cleaved in vivo , for example by enzymatic hydrolysis, to release the biologically active parent. T. Higuchi and V. Stella provide a thorough discussion of the prodrug concept in "Pro-drugs as Novel Delivery Systems", Vol 14 of the A.C.S. Symposium Series, American Chemical Society (1975), which is hereby incorporated herein by reference. Such carboxy protecting groups are well known to those skilled in the art, having been extensively used in the protection of carboxyl groups in the penicillin and cephalosporin fields, as described in U.S. Pat. No. 3,840,556 and 3,719,667, the disclosures of which are hereby incorporated herein by reference. Examples of esters useful as prodrugs for compounds containing carboxyl groups can be found on pages 14-21 of "Bioreversible Carriers in Drug Design: Theory and Application", edited by E.B. Roche, Pergamon Press, New York (1987), which is hereby incorporated herein by reference. Representative carboxy protecting groups are Ci to CQ loweralkyl (e.g., methyl, ethyl or tertiary

butyl and the like); arylalkyl, for example, phenethyl or benzyl and substituted derivatives thereof such as alkoxybenzyl or nitrobenzyl groups and the like; arylalkenyl, for example, phenylethenyl and the like; aryl and substituted derivatives thereof, for example, 5-indanyl and the like; dialkylaminoalkyl (e.g., dimethylaminoethyl and the like); alkanoyloxyalkyl groups such as acetoxymethyl, butyryloxymethyl, valeryloxymethyl, isobutyryloxymethyl, isovaleryloxymethyl, 1-(propionyloxy)-1 -ethyl, 1-(pivaloyloxyl)-1 -ethyl, 1-methyl- 1-(propionyloxy)-1 -ethyl, pivaloyloxymethyl, propionyloxymethyl and the like; cycloalkanoyloxyalkyl groups such as cyclopropylcarbonyloxymethyl, cyclobutylcarbonyloxymethyl, cyclopentylcarbonyloxymethyl, cyclohexylcarbonyloxymethyl and the like; aroyloxyalkyl, such as benzoyloxymethyl, benzoyloxyethyl and the like; arylalkylcarbonyloxyalkyl, such as benzylcarbonyloxymethyl, 2-benzylcarbonyloxyethyl and the like; alkoxycarbonylalkyl or cycloalkyloxycarbonylalkyl, such as methoxycarbonylmethyl, cyclohexyloxycarbonylmethyl, 1-methoxycarbonyl-1- ethyl, and the like; alkoxycarbonyloxyalkyl or cycloalkyloxycarbonyloxyalkyl, such as methoxycarbonyloxymethyl, t-butyloxycarbonyloxymethyl, 1-ethoxycarbonyloxy-1 -ethyl, 1-cyclohexyloxycarbonyloxy-1 -ethyl and the like; aryloxycarbonyloxyalkyl, such as 2-(phenoxycarbonyloxy)ethyl, 2-(5-indanyloxycarbonyloxy)ethyl and the like; alkoxyalkylcarbonyloxyalkyl, such as 2-(1-methoxy-2-methylpropan-2-oyloxy)ethyl and like; arylalkyloxycarbonyloxyalkyl, such as 2-(benzyloxycarbonyloxy)ethyl and the like; arylalkenyloxycarbonyloxyalkyl, such as 2-(3-phenylpropen-2- yloxycarbonyloxy)ethyl and the like; alkoxycarbonylaminoalkyl, such as t-butyloxycarbonylaminomethyl and the like; alkylaminocarbonylaminoalkyl, such as methylaminocarbonylaminomethyl and the like; alkanoylaminoalkyl, such as acetylaminomethyl and the like; heterocycliccarbonyloxyalkyl, such as 4-methylpiperazinylcarbonyloxymethyl and the like; dialkylaminocarbonylalkyl, such as dimethylaminocarbonylmethyl, diethylaminocarbonylmethyl and the like; (5-(ioweralkyl)-2-oxo-1.3-dioxolen-4 ^" -yl)alkyl. such as (5-t-butyl-2-oxo-1,3- dioxolen-4-yl)methyl and the like; and (5-phenyl-2-oxo-1 ,3-dioxolen-4-yl)alkyl, such as (5-phenyl-2-oxo-1 ,3-dioxoIen-4-yl)methyl and the like.

Preferred carboxy-protected compounds of the invention are compounds wherein the protected carboxy group is a loweralkyl, cycloalkyl or arylalkyl ester, for example, methyl ester, ethyl ester, propyl ester, isopropyl ester, butyl ester, sec-butyl ester, isobutyl ester, amyl ester, isoamyl ester, octyl ester, cyclohexyl ester, phenylethyl ester and the like or an alkanoyloxyalkyl, cycloalkanoyloxyalkyl, aroyloxyalkyl or an arylalkylcarbonyloxyalkyl ester.

The term "N-protecting group" or "N-protected" as used herein refers to those groups intended to protect the N-terminus of an amino acid or peptide or to protect an amino group against undersirable reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in Greene, "Protective Groups In Organic Synthesis," (John Wiley & Sons, New York (1981)), which is hereby incorporated by reference. N-protecting groups comprise acyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2- chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o- nitrophenoxyacetyl, α-chlorobutyryl, benzoyl,

4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and the like; sulfonyl groups such as benzenesulfonyl, p-toluenesulfonyl and the like; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl,

3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyloxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimθthoxybenzyloxycarbonyl, 1 - (p-biphenylyl)-l-methylethoxycarbonyl, α,α-dimethyl-3,5-dimethoxybenzyloxycarbonyl, benzhydryloxycarbonyl, t-butyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2,-trichloroethoxycarbonyl, phenoxycarbonyl, 4-nitrophenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl and the like; alkyl groups such as benzyl, triphenylmethyl, benzyloxymethyl and the like; and silyl groups such as trimethylsilyl and the like. Preferred N-protecting groups are formyl, acetyl,

benzoyl, pivaloyi, t-butylacetyl, phenylsulfonyl, benzyl, t-butyloxycarbonyl (Boc) and benzyloxycarbonyl (Cbz).

The term "alkanoyl" as used herein refers to R ₈₅ C(0)- wherein R ₈₅ is a loweralkyl group. The term "alkanoylaminoalkyl" as used herein refers to a loweralkyl radical to which is appended R ₈₆ -NH- wherein R ₈₆ is an alkanoyl group.

The term "alkanoyloxy" as used herein refers to R ₈₇ C(0)-0- wherein R ₈₇ is a loweralkyl group.

The term "alkanoyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended an alkanoyloxy group.

The term "alkenyl" as used herein refers to a branched or straight hydrocarbon chain comprising two to twenty carbon atoms which also comprises one or more carbon-carbon double bonds. Representative alkenyl groups include 2-propenyl (i.e., allyl), 3-methyl-2-butenyl, 3,7-dimethyl-2,6- octadienyl, 4,8-dimethyl-3,7-nonadienyl, 3,7,11-trimethyl-2,6,10-dodecatrienyl and the like.

The term "alkenylene" denotes a divalent group derived from a straight or branched chain hydrocarbon containing from 2 to 10 carbon atoms and also containing at least one carbon-carbon double bond. Examples of alkenylene include -CH=CH-, -CH ₂ CH=CH-. -C(CH ₃ )=CH-. -CH ₂ CH=CHCH ₂ -, and the like. The term "alkoxy" as used herein refers to RO- wherein R is a loweralkyl group. Representative examples of alkoxy groups include methoxy, ethoxy, t- butoxy and the like.

The term "alkoxyalkoxy" as used herein refers to RsoO-R ₈ ιO- wherein R ₈ o is loweralkyl as defined above and R ₈ ι is an alkylene group.

Representative examples of alkoxyalkoxy groups include methoxymethoxy, ethoxymethoxy, t-butoxymethoxy and the like.

The term "alkoxyalkyl" as used herein refers to an alkoxy group as previously defined appended to an alkyl radical as previously defined. Examples of alkoxyalkyl include, but are not limited to, methoxymethyl, methoxyethyl, isopropoxymethyl and the like.

The term "alkoxyalkylcarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₆ -C(0)-0- wherein R ₉₆ is an alkoxyalkyl group.

The term "alkoxycarbonyl" as used herein refers to an alkoxy group as previously defined appended to the parent molecular moiety through a carbonyl group. Examples of alkoxycarbonyl include methoxycarbonyl, ethoxycarbonyl, isopropoxycarbonyl and the like.

The term "alkoxycarbonylalkyl" as used herein refers to an alkoxylcarbonyl group as previously defined appended to a loweralkyl radical. Examples of alkoxycarbonylalkyl include methoxycarbonylmethyl, 2- ethoxycarbonylethyl and the like.

The term "alkoxycarbonylaminoalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₇ -NH- wherein R ₉₇ is an alkoxycarbonyl group. The term "alkoxycarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₃ -0- wherein R ₉₃ is an alkoxycarbonyl group.

The term "alkylamino" as used herein refers to R51NH- wherein R51 is a loweralkyl group, for example, methylamino, ethylamino, butylamino, and the like. The term "alkylaminocarbonylaminoalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₈ -C(0)-NH- wherein R ₉₈ is an alkylamino group.

The term "alkylsulfonyl" as used herein refers to R ₈₂ S(0) ₂ - wherein R ₈₂ is a loweralkyl group. The term "alkylene" denotes a divalent group derived from a straight or branched chain saturated hydrocarbon having from 1 to 10 carbon atoms by the removal of two hydrogen atoms, for example methylene, 1 ,2-ethylene, 1,1- ethylene, 1 ,3-propylene, 2,2-dimethylpropylene, and the like.

The term "alkylsulfonylalkyl" as used herein refers to a loweralkyl radical to which is appended an alkylsulfonyl group.

The term "alkynyl" as used herein refers to a branched or straight hydrocarbon chain comprising two to twenty carbon atoms which also

comprises one or more carbon-carbon triple bonds. Representative alkynyl groups include ethynyl, 2-propynyl (propargyl), 1-propynyl and the like.

The term "amino" as used herein refers to -NH ₂ .

The term "aminoalkyl" as used herein refers a loweralkyl radical to which is appended an amino group.

The term "aroyloxyalkyl" as used herein refers to an alkyl radical which is substituted with R2oC(0)-0- where R2 ₀ is an aryl group. Examples of aroyloxyalkyl groups include benzoyloxymethyl, 1-naphthoyloxymethyl, 2- naphthoyloxymethyl and the like. The term "aryl" as used herein refers to a mono-, bi- or tricyclic carbocyclic ring system comprising 6-14 carbon atoms and having one, two or three aromatic rings including, but not limited to, phenyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl, fluorenyl, anthracenyl, dihydroanthracenyl and the like. Aryl groups can be unsubstituted or substituted with one, two or three substituents independently selected from loweralkyl, haloalkyi, alkoxy, thioalkoxy, amino, alkylamino, dialkylamino, hydroxy, halo, mercapto, nitro, carboxaldehyde, carboxy, alkoxycarbonyl and carboxamide. In addition, substituted aryl groups include tetrafluorophenyl and pentafluorophenyl.

The term "arylalkenyl" as used herein refers to an aryl group as previously defined appended to an alkenyl radical as previously defined. Examples of arylalkenyl include styryl (i.e., 2-phenyletheπyl), 2-(1- naphthyl)ethenyl and the like.

The term "arylalkenyloxycarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₅ -0-C(0)-0- wherein R _g5 is an arylalkenyl group.

The term "arylalkoxy" as used herein refers to R ₈₄ 0- wherein R ₈₄ is an arylalkyl group.

The term "arylalkoxyalkenyl" as used herein refers to an alkenyl radical to which is appended an arylalkoxy group. The term "arylalkyl" as used herein refers to a loweralkyl radical to which is appended an aryl group. Representative arylalkyl groups include benzyl, phenylethyl, hydroxybenzyl, fluorobenzyl, fluorophenylethyl and the like.

The term "arylalkylcarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended an arylalkylcarbonyloxy group (i.e., R ₉₁ C(0)0- wherein R ₉₁ is an arylalkyl group).

The term "arylalkyloxycarbonyloxyalkyP as used herein refers to a loweralkyl radical to which is appended R ₉₂ -0-C(0)-0- wherein R ₉₂ is an arylalkyl group.

The term "arylalkynyl" as used herein refers to an alkynyl radical to which is appended an aryl group.

The term "aryloxy" as used herein refers to R ₈₃ 0- wherein R ₈₃ is an aryl group.

The term "aryloxyalkenyl" as used herein refers to an alkenyl radical to which is appended an aryloxy group.

The term "aryloxyalkyi" as used herein refers to a loweralkyl radical to which is appended an aryloxy group. The term "aryloxycarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₄ -0-C(0)-0- wherein R ₉₄ is an aryl group.

The term "aryl-substituted cycloalkylalkyl" as used herein refers to a cycloalkylalkyl radical in which the alkyl portion of the radical is substituted with an aryl group. Examples of aryl-substituted cycloalkylalkyl include α-(cyclopropylmethyl)benzyl, α-(cyclobutylmethyl)benzyl and the like.

The term "carboxaldehyde" as used herein refers to the group -C(0)H. The term "carboxamide" as used herein refers to the group -C(0)NH2. The term "carboxyalkyl" as used herein refers to a loweralkyl radical to which is appended a carboxy (-COOH) group. The term "cycloalkanoyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended a cycloalkanoyloxy group (i.e., R ₈₈ -C(0)0- wherein R ₈₈ is a cycloalkyl group).

The term "cycloalkyl" as used herein refers to an alicyclic group comprising from 3 to 10 carbon atoms including, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, norbomyl, adamantyl and the like.

The term "cycloalkylalkyl" as used herein refers to a loweralkyl radical to which is appended a cycloalkyl group. Representative examples of cycloalkylalkyl include cyclopropylmethyl, cyclohexylmethyl, 2- (cyclopropyl)ethyl, adamantylmethyl and the like. The term "cycloalkyloxycarbonylalkyl" as used herein refers to a loweralkyl radical to which is appended R ₈₉ -0-C(0)- wherein R ₈₉ is a cycloalkyl group.

The term "cycloalkyloxycarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₀ -O-C(O)-O- wherein R ₉₀ is a cycloalkyl group.

The term "dialkylamino" as used herein refers to R56R57N- wherein R56 and R57 are independently selected from loweralkyl, for example dimethylamino, diethylamino, methyl propylamino, and the like.

The term "dialkylaminoalkyl" as used herein refers to a loweralkyl radical to which is appended a dialkylamino group.

The term "dialkyaminocarbonylalkyl" as used herein refers to a loweralkyl radical to which is appended R ₉₉ -C(0)- wherein R ₉₉ is a dialkylamino group.

The term "haloalkyi" as used herein refers to a lower alkyl radical, as defined above, bearing at least one halogen substituent, for example, chloromethyl. fluoroethyl or trifluoromethyl and the like.

The term "halogen" or "halo" as used herein refers to I, Br, Cl or F. The term "heterocyclic ring" or "heterocyclic" or "heterocycle" as used herein refers to any 3- or 4-membered ring containing a heteroatom selected from oxygen, nitrogen and sulfur; or a 5-, 6- or 7-membered ring containing one, two or three heteroatoms independently selected from the group consisting of nitrogen, oxygen and sulfur or a 5-membered ring containing 4 nitrogen atoms; and includes a 5-, 6- or 7-membered ring containing one, two or three nitrogen atoms; one oxygen atom; one sulfur atom; one nitrogen and one sulfur atom; one nitrogen and one oxygen atom; two oxygen atoms in non-adjacent positions; one oxygen and one sulfur atom in non-adjacent positions; two sulfur atoms in non-adjacent positions; two sulfur atoms in adjacent positions and one nitrogen atom; two adjacent nitrogen atoms and one sulfur atom; two non- adjacent nitrogen atoms and one sulfur atom; two non-adjacent nitrogen atoms

and one oxygen atom. The 5-membered ring has 0-2 double bonds and the 6- and 7-membered rings have 0-3 double bonds. The nitrogen heteroatoms can be optionally quatemized. The term "heterocyclic" also includes bicyclic groups in which any of the above heterocyclic rings is fused to a benzene ring or a cyclohexane ring or another heterocyclic ring (for example, indolyl, quinolyl, isoquinolyl, tetrahydroquinolyl, benzofuryl or benzothienyl and the like). Heterocyclics include: azetidinyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, pyrazolyl, pyrazolinyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, piperidinyl, homopiperidinyl, pyrazinyl, piperazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, furyl, thienyl, thiazolidinyl, isothiazolyl, triazolyl, tetrazolyl, isoxazolyl, oxadiazolyl, thiadiazolyl, pyrrolyl, pyrimidyl and benzothienyl. Heterocyclics also include compounds of the

formula where X ^* is -CH2- or -0- and Y ^* is -C(O)- or

[-C(R")2-]v where R" is hydrogen or Cι-C4-alkyl and v is 1 , 2 or 3 such as 1 ,3- benzodioxolyl, 1 ,4-benzodioxanyl and the like.

Heterocyclics can be unsubstituted or monosubstituted or disubstituted with substituents independently selected from hydroxy, halo, oxo (=0), alkylimino (R*N= wherein R* is a loweralkyl group), amino, alkylamino, dialkylamino, alkoxy, alkoxyalkoxy, haloalkyi, cycloalkyl, aryl, arylalkyl, -COOH,

-SO ₃ H and loweralkyl. In addition, nitrogen containing heterocycles can be N- protected.

The term "(heterocyclic)alky as used herein refers to a heterocyclic group as defined above appended to a loweralkyl radical as defined above.

Examples of heterocyclic alkyl include 2-pyridylmethyl, 4-pyridylmethyl, 4- quinolinylmethyl and the like.

The term "heterocycliccarbonyloxyalkyl" as used herein refers to a loweralkyl radical to which is appended R ₁₀₀ -C(O)-O- wherein R ₁₀₀ is a heterocyclic group.

The term "heterocyclic-substituted cycloalkylalkyl" as used herein refers to a cycloalkylalkyl radical in which the alkyl portion of the radical is substituted with a heterocyclic group. Examples of heterocyclic-substituted cycloalkylalkyl include α-(cyclopropylmethyl)furan-2-ylmethyl, α-(cyclobutylmethyl)thien-2- ylmethyl and the like.

The term "hydroxyalkyl" as used herein refers to a loweralkyl radical to which is appended a hydroxy (-OH) group.

The term "loweralkyl" as used herein refers to branched or straight chain alkyl groups comprising one to ten carbon atoms, including methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, neopentyl and the like.

The term "thioalkoxy" as used herein refers to R70S- wherein R70 is loweralkyl. Examples of thioalkoxy include, but are not limited to, methylthio, ethylthip and the like.

The term "thioalkoxyalkyi" as used herein refers to a thioalkoxy group as previously defined appended to a loweralkyl radical as previously defined.

Examples of thioalkoxyalkyi include thiomethoxymethyl, 2-thiomethoxyethyl and the like.

Preferred compounds of the invention are selected from the group consisting of:

5-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]benzene-1 ,2,4- tricarboxylic acid; 2,5-Di[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl] benzene- 1 ,4-dicarboxylic acid;

4,6-Di[N-benzyl-Nτ(4-phenoxybenzyl)aminocarbonyl] benzene-

1 ,3-dicarboxylic acid; 5-[N-Phenethyl-N-(4-phenoxybenzyl)diaminocarbonyl]benzene- 1 ,2,4-tricarboxylic acid; 4-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]benzene-1 ,2- dicarboxylic acid;

5-[N,N-Di(4-phenoxybenzyl)aminocarbonyl]benzene-1 ,2.4- tricarboxylic acid;

5-[N-(4-Phenoxybenzyl)-N-(4-phenylbutyl)aminocarbonyl]- benzene-1 ,2,4-tricarboxylic acid; 5-[N-(3,4-Dichlorobenzyl)-N-(4-phenoxybenzyl)aminocarbonyl]- benzene-1 ,2,4-fricarboxylic acid; 4-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]benzene-1 ,3- dicarboxylic acid; 5-[N-Benzyl-N-(2-(4-phenoxyphenyl)ethyl)aminocarbonyl]- benzene-1 ,2,4-tricarboxylic acid; 4-{[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]amino}benzene- 1 ,2- dicarboxylic acid;

4-{[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]oxy}benzene -1,2- dicarboxylic acid; 4-{[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]methyl}-1,2-be nzene dicarboxylic acid; and 5-[N-(2-Ethoxybenzyl)-N-(3-(4-methylphenoxy)benzyl)aminocarb onyl]- benzene-1 ,2,4-tricarboxylic acid;

or a pharmaceutically acceptable salt thereof.

Particularly preferred is the compound 5-[N-(2-Ethoxybenzyl)-N-(3-(4- methylphenoxy)benzyl)aminocarbonyl]-benzene-1 ,2,4-tricarboxylic acid, or a pharmaceutically acceptable salt thereof.

ln general, the compounds of the invention can be prepared by the processes illustrated in Schemes l-VIII. In the schemes, the preparation of compounds of the formula (I) are shown as representative examples. Similar methods can be applied to the preparation of compounds of the formula (II), (III) and (IV).

According to reaction Scheme I, i ,2,4,5-benzenetetracarboxylic dianhydride in an inert solvent such as acetone and acetonitrile or toluene is treated with one equivalent of HNR1R2 (where R1 and R2 are as defined previously herein) in the presence of an aprotic base such as triethylamine or diisopropylethylamine followed by hydrolysis of the second anhydride (for example, using hydrochloric acid in methylene chloride) gives amide 2.

Compound 2 in an inert solvent such as THF in the presence of an aprotic base such as triethylamine can be reacted with one equivalent of HNR1R2 (where R1 and R2 are as defined previously herein), followed by hydrolysis of the anhydride (for example, using hydrochloric acid in methylene chloride), to give amide 4. Other substitution patterns can be obtained starting from the appropriately substituted mono- or dianhydrides.

Phthalic anhydride 5 can be reacted with HNR1R2 (where R1 and R2 are as defined previously herein) in toluene in the presence of an aprotic base such as triethylamine to give mono-amide mono-carboxylic acid f>. Other substitution patterns can be obtained starting from the appropriately substituted mono- or dianhydrides.

The preparation of compounds having a 2,5- or 2,4-diamide substitution pattern is shown in Scheme II. Treating 1 ,2,4,5-benzenetetracarboxylic dianhydride (1) in an inert solvent such as acetone and acetonitrile or toluene with greater than 2 equivalents of HNR1R2 (where R1 and R2 are as defined previously herein) in the presence of an aprotic base such as triethylamine or diisopropylethylamine, followed by hydrolysis of the second anhydride (for example, using hydrochloric acid in methylene chloride) when only one anhydride reacts, gives a mixture of amide 2 and diamides 10 and H which are separable by column chromatography.

The preparation of compounds having a 1 ,3- or 1 ,4-dicarboxylic mono- amide substitution pattern is shown in Scheme III. Treating 1,2,4- benzenetricarboxylic anhydride (12) in an inert solvent such as acetone and acetonitrile or toluene with one equivalent of HNRi R2 (where R1 and R2 are as defined previously herein) in the presence of an aprotic base such as triethylamine or diisopropylethylamine affords mono-amide dicarboxylic acids 13 and 14 which are separable by column chromatography.

The preparation of compounds having a 1 ,2- or 1 ,3-dicarboxylic acid mono-amide substitution pattern is shown in Scheme IV. Treating 1 ,2,3- benzenetricarboxylic anhydride (15) in an inert solvent such as acetone and acetonitrile or toluene with one equivalent of HNR1R2 (where R1 and R2 are as defined previously herein) in the presence of an aprotic base such as triethylamine or diisopropylethylamine affords mono-amide dicarboxylic acids 16 and 17 which are separable by column chromatography. The preparation of compounds having a 1 ,3-dicarboxylic acid 5-amide substitution pattern is shown in Scheme V. Benzene-1 ,3,5-tricarboxylic acid triester (18) (wherein R is loweralkyl) in a solvent system such as methanol and THF is treated with one equivalent of a base such as potassium hydroxide or lithium hydroxide to give the mono-carboxylic acid diester 19. The carboxylic acid functionality is activated (for example by treatment with oxalyl chloride) to give compound 20 (wherein X' is a leaving group such as chloro). The activated acid (20) is then reacted with HNR1R2 (where R1 and R2 are as defined previously herein) in the presence of an aprotic base such as triethylamine or diisopropylethylamine to give amide 21. Ester hydrolysis (for example, using lithium hydroxide in methanol and THF) affords the dicarboxylic acid 22.

The preparation of a compound having a nucleophilic linking group X to the benzene ring is shown in Scheme VI. The appropriate carboxylic acid R2CO2H (where R2 is as defined previously) is converted to its acid chloride 8 (for example, using oxalyl chloride and a catalytic amount of DMF). The acid chloride can then be reacted with the appropriately substituted benzene 7 to give compound 9.

96/34851

-29-

A preferred process for the preparation of compounds wherein X is NH is shown in Scheme VII. The nitro group of diester 23 (wherein R is loweralkyl) is reduced (for example, using ammonium formate with a palladium on carbon catalyst and the like) to give amine 24. Carbamoyl chloride 2ϋ is prepared from HNR1R2 (where R1 and R are as defined previously herein) and phosgene in an inert solvent such as benzene or toluene. The amine 24 and carbamoyl chloride 23. in the presence of 4-dimethylaminopyridine in pyridine react to give urea 26. Ester hydrolysis (for example, using sodium hydroxide in methanol and THF) affords the desired dicarboxylic acid £7_. A preferred process for the preparation of compounds wherein X is O is also shown in Scheme VII. Hydroxyphthalate 2£ (wherein R is loweralkyl) is treated with a base such as sodium hydride in diglyme and then reacted with carbamoyl chloride 25 to give carbamate £9. Ester hydrolysis (for example, using sodium hydroxide in methanol and THF) affords the desired dicarboxylic acid 3Q.

A preferred process for the preparation of compounds wherein X is CH ₂ is shown in Scheme VIII. Diester SI (wherein R is loweralkyl) is brominated (for example, using N-bromosuccinimide in the presence of 2,2'- azobisisobutyronitrile in carbon tetrachloride) to give the bromomethyl compound 32. Treatment of compound 3 > with tris(benzotriazol-1-yl)methane by the method described in Synthesis 666 (1990), which is incorporated herein by reference, affords compound 33 (wherein BT is benzotriazol-1-yl). Compound 33 is converted to carboxy compound 34 by the procedure also described in Synthesis 666 (1990). The carboxy functionality of compound 34 is activated (for example, by treatment with oxalyl chloride to give the acid chloride) and then reacted with HNR1R2 (where R1 and R2 are as defined previously herein) to give amide 35- Ester hydrolysis (for example, using sodium hydroxide in methanol and THF) affords the desired dicarboxylic acid 26.

SCHEME I

SCHEME II

12

11

SCHEME III

12

12 14

SCHEME IV

15

SCHEME V

Ifi 13

21 20.

22

SCHEME VI

X = O, S, N(R _a )

SCHEME VII

22. 24

21

26

2Ω

SCHEME VIII

ai 22

24

33

25 3S

The foregoing may be better understood by reference to the following examples which are provided for illustration and not intended to limit the scope of the inventive concept. The following abbreviations were used AgOBn for silver benzoate, BOP-

Cl for bis(2-oxo-3-oxazolidinyl)phosphinic chloride, n-BuLi for n-butyl lithium, DIBAL for diisobutylaluminum hydride, DMAP for dimethylaminopyridine, DME for dimethoxyethane, DMF for dimethylformamide, DMSO for dimethylsulfoxide, EDCI for 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, Et3N for triethylamine, Et2θ for diethyl ether, EtOAc for ethyl acetate, EtOH for ethanol, HOAc for acetic acid, HOBT for 1-hydroxybenzotriazole, LAH for lithium aluminum hydride, LDA for lithium diisopropylamide, MeOH for methanol, Pd/C for palladium on carbon, THF for tetrahydrofuran, and TPAP for tetrapropylammonium perruthenate.

Example 1 5- N-Benzyl-N-f4-phenoxybenzyπaminocarbonyl1benzene-1.2.4-tric arboxylic acid

Example 1A N-Benzyl-N-(4-phenoxybenzyπamine 4-Phenoxybenzaldehyde (5.0 g, 25.2 mmol) and benzylamine (1.46 g, 26.5 mmol) were dissolved in 100 mL of 1% acetic acid in methanol under an atmosphere of dry nitrogen. Sodium cyanoborohydride (1.66 g, 26.5 mmol) was added, and stirring was continued for 18 hours at which time the solvent was removed under reduced pressure. The residue was suspended in ether, washed with 5% NaHCθ ₃ and brine, and dried over Na2S04 to give the title compound.

Example 1B 5-[ ^" N-Benzyl-N-(4-phenoxybenzvhaminocarbonvπbenzene-1.2.4 -tricarboxylic acid A solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (1.2 g, 5.6 mmol), th compound resulting from Example 1A (3.6 g, 12.4 mmol) and Et^N (1 mL) in toluene (25 mL) was refluxed for 2.5 hours and then left at room temperature overnight. The reaction mixture was then concentrated under reduced pressure. The residue was then taken up in EtOAc, washed with 10% HCI (3x) and a saturated NaCI solution, dried (MgS04), and evaporated. The residue containing a mixture of isomers was flash silica gel chromatographed eluting with 18:1:1 EtOAc-H ₂ 0-HC0 H afforded the title compound. 1H NMR (300 MHz, DMSO-d ₆ ) d 4.20 (d, 4H), 6.80-7.50 (m, 14H), 7.65 (d, 1H), 8.25 (d,1 H). MS (FAB)+ m/e 526 (M+H)+. (FAB)" m/e 524 (M-H) ^" .

Example 2

2.5-DirN-benzyl-N-(4-phenoxybenzvhaminocarbonvπ benzene-1.4-dicarboxylic acid Also eluting from the column described in Example 1 B was the title compound. ^" -H NMR (300 MHz, DMSO-d ₆ ) d 4.20 (d, 8H), 6.80-7.50 (m, 28H), 7.65 (d, 1 H), 8.25 (d,1 H). MS (FAB)+ m/e 797 (M+H) ⁺ . (FAB)- m/e 795 (M-H)-.

Example 3 4.6-DirN-benzyl-N-f4-phenoxybenzvhaminocarbonvπbenzene-1.3- dicarboxylic acid Also eluting from the column described in Example 1 B was the title compound. ^" Η NMR (300 MHz, DMSO-d ₆ ) d 4.20 (d, 8H), 6.80-7.50 (m, 28H), 7.65 (d, 1H), 8.25 (d,1H). MS (FAB)+ m/e 797 (M+H)+. (FAB) ^" m/e 795 (M-H)-.

Example 4

Alternate Preparation of

5-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonvπbenzene-1.2. 4-tricarboxyliπ acid To a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.98 g, 4.4 mmol) in 40 mL of acetone and 10 mL acetonitrile cooled in a salt-ice bath was added a solution of the compound resulting from Example 1A (1.3 g, 4.4 mmol) and diisopropylethyl amine (0.57 g , 4.4 mmol) dissolved in 10 mL acetone dropwise over 5 hours via syringe pump. After stirring an additional hour, the reaction mixture was evaporated under reduced pressure at room temperature. 6 N HCI (90 mL) and methylene chloride (10 mL) were added to the reaction mixture, which was then stirred at room temperature for 1 hour. The solid which formed was then filtered off. Flash silica gel chromatography eluting with with 95:4:1 CHCI ₃ -MeOH-HOAc yielded 1.81 g (76.5%) of the title compound. ^" Η NMR (300 MHz, DMSO-d ₆ ) d 4.20 (d, 4H), 6.80-7.50 (m, 14H), 7.65 (d, 1 H), 8.25 (d,1H). MS (FAB)+ m/e 526 (M+H)+. (FAB)" m/e 524 (M-H)-.

Example 5 2-[N-Benzyl-N-f4-phenoxybenzyl)aminocarbonyl]benzoic acid A solution of phthalic anhydride (0.41 g, 2.8 mmol), the compound resulting from Example 1A (0.81 g, .8 mmol) and Et3N (0.5 mL) in toluene (50 mL) was refluxed for 24 hours. The reaction mixture was then concentrated under reduced pressure. The residue was taken up in EtOAc, washed with 5% HCI (3x) and saturated NaCI solution, dried (MgSO^, and evaporated. Flash silica gel chromatography eluting with 95:4:1 CH2Cl2-MeOH-HOAc yielded 0.32 g of the title compound. ¹ H NMR (300 MHz, DMSO-d ₆ ) d 4.20 (d, 4H), 6.80-8.0 (m, 18H). MS (FAB)+ m/e 438 (M+H)+.

Example 6 2.5-Di[N-phenethyl-N-f4-phenoxybenzvπaminocarbonvπbenzene- 1.4- dicarboxylic acid The title compound was prepared by the procedures described in Example 1B using N-phenethyl-N-(4-Phenoxybenzyl)amine in place of N-

benzyl-N-(4-phenoxybenzyl)amine. ^" -H NMR (300 MHz, DMSO-d ₆ ) d 2.70-3.20 (m, 8H), 4.30 (m, 4H), 6.80-7.50 (m, 30H). MS (FAB)+ m/e 825 (M+H)+. (FAB)- m/e 823 (M-H)".

Example 7

4.6-DirN-phenethyl-N-f4-phenoxybenzvπdiaminocarbonvHbenz ene-1.3- dicarboxylic acid The title compound was prepared by the procedures described in Example 1B using N-phenethyl-N-(4-Phenoxybenzyl)amine in place of N- benzyl _: N-(4-phenoxybenzyl) amine. ¹ H NMR (300 MHz, DMSO-d ₆ ) d 2.70-3.20 (m, 8H), 4.30 (m, 4H), 6.80-7.50 (m, 30H). MS (FAB)" m/e 823 (M-H)-.

Example 8 5-fN-Phenethyl-N-(4-phenoxybenzy0diaminocarbonyr|benzene-1.2 .4- tricarboxylic acid

The title compound was prepared by the procedures described in Example 4 using N-phenethyl-N-(4-Phenoxybenzyl)amine in place of N-benzyl- N-(4-phenoxybenzyl) amine. ¹ H NMR (300 MHz, DMSO-d ₆ ) d 2.70-3.20 (m, 4H), 4.30 (m, 2H), 6.80-7.50 (m, 14H). MS (FAB)+ m/e 540 (M+H)+. (FAB) ^" m/e 538 (M-H)-.

Example 9 4-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonyl]benzene-1.2-dic arboxylic acid To a cold solution of 4-chloroformylphthalic anhydride (1.09 g, 5.2 mmol), prepared by the method described in J. Org. Chem. 38: 2257 (1973), in 40 mL of tetrahydrofuran was added dropwise a solution of Et3N (0.52 g, 5.2 mmol) and N-benzyl-N-(4-phenoxybenzyl)amine (1.5 g, 5.2 mmol) dissolved in tetrahydrofuran (20 mL). The solution was stirred and allowed to come to room temperature overnight. The reaction was then concentrated in vacuo and treated with 6 N HCI (90 mL) and methylene chloride (10 mL) and stirred at room temperature for 1 hour. The mixture was extracted with EtOAc and the combined organic extracts washed with 5% HCI and saturated NaCI solution, dried over magnesium sulfate and concentrated in vacuo. Flash silica gel

chromatography eluting with 95:4:1 CH2Cl2-MeOH-HOAc yielded 0.8 g of the title compound. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 4.20 (d, 4H), 6.80-7.50 (m, 15H), 7.65 (d, 1H), 8.25 (d, 1H). MS (FAB)+ m/e 482 (M+H)+ and (FAB)- m/e 480 (M-H)-.

Example 10 5-fN.N-Dif4-phenoxybenzvhaminocarbonyπbenzene-1.2.4-tricarb oxylic acid The title compound was prepared by the procedures described in Example 4, using N,N-di(4-phenoxybenzyl)amine in place of N-benzyl-N-(4- phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 4.20 (d, 4H), 6.80-7.50 (m, 18H), 7.65 (s, 1H), 8.25 (s, 1H). MS (FAB)+ m/e 618 (M+H)+ and (FAB)- m e 616 (M-H)-.

Example 11 5-[N-f4-Phenoxybenzvπ-N-f4-phenylbutvπaminocarbonvπbenzen e-1.2.4- tricarboxylic acid The title compound was prepared by the procedures described in Example 4, using N-(4-phenylbutyl-N-(4-phenoxybenzyl)amine in place of the N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 1.20- 1.70 (m, 4H), 2.25-3.00 (m, 4H), 4.30 (m, 2H), 6.80-7.50 (m, 14H). MS (FAB)+ m/e 568 (M+H)+ and (FAB)" m/e 566 (M-H)"-

Example 12 5-fN.N-Dibenzylaminocarbonyhbenzene-1.2.4-tricarboxylic acid The title compound was prepared by the procedures described in

Example 4, using N,N-dibenzylamine in place of the N-benzyl-N-(4- phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 4.20 (s, 4H), 7.10-7.40 (m, 10H), 7.65 (s, 1 H), 8.25 (s,1H). MS (FAB)+ m/e 434 (M+H)+ and (FAB)" m/e 432 (M-H) ^" -

Example 13 5-fN-H -Adamantylmethvh-N-f4-Dhenoxybenzyh aminocarbonyllbenzene-1.2.4- tricarboxylic acid The title compound was prepared by the procedures described in Example 4, using N-(1-adamantyl methyl)-N-(4-phenoxybenzyl)amine in place of the N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 0.90-2.00 (m, 3H), 3.20-4.00 (m, 14H), 4.30 (m, 2H), 6.80-7.50 (m, 11H). MS (FAB)+ m/e 584 (M+H)+ and (FAB)" m/e 582 (M-H)"-

Example 14

3-fN-Benzyl-N-( -phenoxybenzyl)aminocarbonvπbenzoic acid To a solution of isophthaloyl dichloride (0.73 g, 3.6 mmol) in 20 mL of tetrahydrofuran was added N-benzyl-N-(4-phenoxybenzyl)amine (1.56 g, 5.4 mmol) and triethylamine (0.52g, 5.4 mmol). The reaction was then refluxed for 2 hours, cooled to room temperature and concentrated in vacuo. To the residue was added 10 % HCI, and the reaction mixture was stirred for 10 minutes and extracted with ethyl acetate. The combined organic extracts were dried (MgSθ4), concentrated in vacuo and purified by flash silica gel chromatography eluting with with 95:4:1 CHCl3-MeOH-HOAc to afford the title compound. ¹ H NMR (DMSO-de, 300 MHz) d 4.20 (d, 4H), 6.80-7.50 (m, 14H), 7.60 (m, 1 H), 7.70 (d, 1 H), 8.00 (m, 2H). MS (FAB)+ m/e 438 (M+H)+ and (FAB)" m/e 436 (M- H)-.

Example 15 4.5-Dimethoxy-2-[N-^4-Phenoxybenzvπcarbonylamino1benzoic acid methyl ester To a solution of 4-phenoxyphenylacetic acid (1.14 g, 5 mmol) dissolved in oxalyl chloride (10 mL.) was added a catalytic amoumt of dimethylformamide. The reaction mixture was refluxed for 1 hour and then cooled to room temperature and evaporated. Toluene (2 x 20 mL) was added and evaporated to remove the excess oxalyl chloride. The resulting 4-phenoxyphenylacetyl chloride was then added to a solution of 2-methoxycarbonyl-4,5- dimethoxyaniline (1.0 g, 4.7 mmol) in toluene (20 mL). The reaction was then

stirred at room temperature overnight and evaporated. Water and ethyl acetate were added, the phases were separated, and the organic phase was washed with 5 % NaOH and a saturated solution of NaCI, dried (MgSθ4), and evaporated. Flash silica gel chromatography eluting with with 95:4:1 CHCI3- MeOH-HOAc yielded 1.8 g of the title compound. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 3.75 (s, 3H), 3.80 (d, 7H), 7.00-7.15 (m, 5H), 7.40 (m, 5H), 8.20 (s, 1H). MS (FAB)+ m/e 422 (M+H)+ .

Example 16 4.5-Dimethoxy-2-rN-(4-phenoxybenzvπcarbonylaminolbenzoic acid

A solution of the compound resulting from Example 15 (0.65 g, 1.3 mmol) and lithium hydroxide monohydrate (0.110 g, 2.6 mmol) in H2O (10 mL) and tetrahydrofuran (15 mL) was stirred at room temperature for 24 hours. The volatiles were removed under reduced pressure, water was added, and the aqueous solution was washed with ethyl acetate. The aqueous phase was cooled and acidified with 10% HCI and then extracted with ethyl acetate. The combined extracts were washed with saturated sodium chloride solution, dried over magnesium sulfate and concentrated in vacuo to afford 600 mg of the title compound. ¹ H NMR (DMSO-d _6l 300 MHz) d 3.75 (d, 4H), 3.80 (s, 3H), 7.00- 7.15 (m, 5H), 7.40 (m, 5H), 8.20 (s, 1 H). MS (FAB)+ m/e 408 (M+H)+ .

Example 17 5-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl)-1.2.4- triethoxycarbonylbenzene A solution of the compound resulting from Example 4 (0.30 g, 0.6 mmol), diisopropylamine (0.23 g, 1.8 mmol) and 1.0 M triethyloxonium tetrafluoroborate in dichloromethane (24.0 mL, 2.4 mmol) in 20 mL of methylene chloride was stirred at room temperature for 24 hours. The mixture was washed with 10% HCI (3x), NaHCθ3 (3x) and saturated NaCI solution, dried (MgSθ4) and evaporated, to give 0.15 g of an amber viscous residue. The oil was purified by flash silica gel chromatography eluting with 5% EtOH in CH2CI2 to give 0.11 g of the title compound. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 1.25 (m, 9H), 4.28 (m,

6H), 4.65 (d, 4H), 6.80-7.50 (m, 14H), 7.65 (d, 1 H), 8.25 (d, 1 H). MS (FAB)+ m/e 610 (M+H)+.

Example 18 2-[N-Benzyl-N-f4-phenoxybenzyπaminocarbonyl]-4.5-dimethoxyb enzoic acid A solution containing 4,5-dimethoxyphthalic anhydride (1.34 g, 6.4 mmol), N-benzyl-N-(4-phenoxybenzyl)amine (1.85 g, 6.4 mmol), and diisopropylethylamine (0.83 g, 6.4 mmol) in toluene (30 mL) was refluxed for 2 hours. The reaction mixture was then evaporated, and the residue was then taken up in EtOAc, washed with 5% HCI (3x) and saturated sodium chloride solution, dried over magnesium sulfate and evaporated. Flash silica gel chromatography eluting with 95:4:1 CH2θ2-MeOH-HOAc yielded 1.45 g of the title compound. 1H NMR (DMSO-d ₆ , 300 MHz) d 3.60 (d, 3H), 3.80 (d, 3H), 4.20 (m, 4H), 6.80-7.50 (m, 16H). MS (FAB)+ m/e 498 (M+H)+ .

Example 19 5-rN-(3.4-Dichlorobenzyπ-N-(4-phenoxybenzv0aminocarbonyl]be nzene-1.2.4- tricarboxylic acid

The title compound was prepared by the procedures described in Example 4, using N-(3.4-dichlorobenzyl)-N-(4-phenoxybenzyl)amine in place of

N-benzyl-N-(4-phenoxybenzyl)amine. ^" >H NMR (DMSO-d ₆₎ 300 MHz) d 4.20 (d,

4H) 6.80-7.50 (m, 12H), 7.65 (d, 1H), 8.25 (d,1H). MS (FAB)+ m/e 594 (M+H)+ and (FAB)" m/e 592 (M-H)"-

Example 20

4-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl]benzene-1.3- dicarboxylic acid and 2-rN-Benzyl-N-^4-phenoxybenzvhaminocarbonyl]benzene-1.4-dica rboxylic acid The title compounds were prepared by the procedures described in Example 4, using 1 ,2.4-benzenetricarboxylic anhydride in place of 1 ,2,4,5- benzenetetracarboxylic dianhydride. ¹ H NMR (DMSO-dδ, 300 MHz) d 4.20 (d, 4H) 6.85-7.50 (m, 14H), 7.60 (d, 1H), 8.10 (m,1H), 8.50 (m,1H). MS (FAB)+ m/e 482 (M+H)+ and (FAB)" m e 480 (M-H) ^"

Example 21 3-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1benzene-1.2-dic arboxylic acid and 2-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonyπbenzene-1.3-dic arboxylic acid The title compounds were prepared by the procedures described in Example 4, using 1 ,2,3-benzenetricarboxylic anhydride in place of 1 ,2,4,5- benzenetetracarboxylic dianhydride. ¹ H NMR (DMSO-dβ, 300 MHz) d 4.20 (d, 4H) 6.80-7.50 (m, 15H), 7.65 (t, 1H), 8.20 (d,1H). MS (FAB)+ m/e 482 (M+H)+ and (FAB)" m/e 480 (M-H)"

Example 22 5-fN-Benzyl-N-f2-f4-phenoxyphenvπethyπaminocarbonyl]benzen e-1.2.4- tricarboxylic acid

The title compound was prepared by the procedures described in Example 4, using N-benzyl-N-(2-(4-phenoxyphenyl)ethyl)amine in place of 1 ,2,4,5-benzenetetracarboxylic dianhydride. ¹ H NMR (DMSO-d _δ , 300 MHz) d 4.20 (d, 4H) 6.80-7.50 (m, 12H), 7.65 (d, 1H), 8.25 (d,1H). MS (FAB)+ m/e 540 (M+H)+ and (FAB)" m/e 539 (M-H)"-

Example 23

5-rN-f3.4-Dichlorobenzvπ-N-f2-f2-naphthoyloxy ⁾ ethvπaminocarbonyl]benzene-

1 ,2.4-tricarboxylic acid

Example 23A N-(3.4-Dichlorobenzyπ-N-f2-f2-naphthoyloxy)ethyπamine A solution of 3,4-dichlorobenzaldehyde (8.75 g, 0.05 mol), 2- aminoethanol (3.05 g, 0.05 mol), and a catalytic amount of p-toluenesulfonic acid in ethanol (100 mL) was refluxed for 6 hours. The reaction was cooled, sodium borohydride (1.89 g, 0.05 mol) was added, and the reaction mixture was stirred at room temperature overnight. Water (50 mL) was added, stirring was continued for an additional 30 minutes, and the mixture was extracted with

EtOAc. The combined organic extracts were washed with saturated sodium chloride solution, dried over magnesium sulfate and evaporated to give 7.80 g of N-(3,4-dichlorobenzyl)ethanolamine. ¹ H NMR (CDCI ₃ , 300 MHz) d 3.1 (t, 2H), 3.82 (t, 2H), 4.10 (s, 2H), 7.30 - 8.30 (m, 3H). To a stirred solution of the ethanolamine prepared above (1.1 g, 5 mmol),

2-naphthoic acid (0.86 g, 5 mmol) and triethylamine (0.5 g, 5 mmol) in CH2CI2 (30 mL) was added EDCI (0.96 g, 5 mmol). The reaction was stirred for 4 hours and then treated with H2O. The organic layer was separated, washed with 10% NaOH (20 mL) and saturated NaCI solution (50 mL), dried (MgS04), and evaporated, to give 0.8 g of the title compound as an oil. ¹ H NMR (CDCI3, 300 MHz) d 3.1 (t, 2H), 3.82 (t, 2H), 4.10 (s, 2H), 7.30 - 8.30 (m, 10H). MS (FAB)+ m/e 374 (M+H)+.

Example 23B 5-[N-f3.4-Dichlorobenzvπ-N-(2-f2-naphthoyloxy)ethvπaminoca rbonyl1benzene-

1.2.4-tricarboxylic acid To a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (1.00 g, 4.5 mmol) in 140 mL of acetone cooled in a salt-ice bath was added a solution of the compound resulting from Example 23A (1.70 g, 4.5 mmol) and diisopropylethylamine (0.84 g , 6.3 mmol) in 10 mL of acetone dropwise over 5 hours via syringe pump. After stirring an additional hour the reaction mixture was evaporated under reduced pressure at room temperature. To the residue was added 6 HCI (90 mL) and methylene chloride (10 mL), and the reaction mixture was stirred at room temperature for 1.5 hours. The solid which formed was then filtered off. Flash silica gel chromatography eluting with with 18:1:1 EtOAc-H ₂ 0-HC0 H yielded 0.47 g of the title compound. ¹ H NMR (DMSO-d ₆ , 300 MHz) d 3.80 (m, 2H), 4.20-4.90 (m, 4H), 7.20-8.10 (m, 12H). MS (FAB)+ m/e 610 (M+H)+ and (FAB)- m/e 608 (M-H) ^" -

Example 24 4-rN-Benzyl-N-f3-phenoxybenzvhaminocarbonvnbenzene-1.2-dicar boxylic acid

A solution of triethylamine (0.51 g, 5 mmol) and N-benzyl-N-(3- phenoxybenzyl)amine, prepared by the procedure of Example 1A starting from 3-phenoxybenzaldehyde instead of 4-phenoxybenzaldehyde, (1.45 g, 5 mmol) in THF (30 mL) was added dropwise to a stirred solution of 4-chloroformyl- phthalic anhydride, prepared by the procedure described in J. Org. Chem. 38: 2257 (1973), (1.05 g, 5 mmol) in THF (30 mL) at 0 °C. After the addition was complete, the reaction mixture was slowly allowed to warm to ambient temperature overnight. The reaction mixture was concentrated in vacuo at 20 °C, and the residue obtained was treated with methylene chloride (10 mL) and 6 N HCI (90 mL) and stirred at ambient temperature for 2 hours. The organic layer was separated, and the aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic extracts were washed successively with 5% HCI, cold water and saturated sodium chloride solution, dried over MgSθ4 and concentrated under reduced pressure. The liquid obtained was chromatographed on silica gel eluting with 84.5:13.5:2 CHCtø-MeOH-HOAc to give the product as a mono-methyl ester. The ester was hydrolyzed by refluxing overnight with lithium hydroxide (1.8 g, 43 mmol) in THF (20 mL) and water (12 mL). The reaction mixture was concentrated under reduced pressure to remove the THF, diluted with cold water (20 mL), acidified with dilute HCI, and extracted with ethyl acetate (3 x 50 mL). The combined organic extracts were washed with cold water and saturated sodium chloride solution, dried over sodium sulfate and concentrated under reduced pressure. The residue obtained was chromatographed on silica gel eluting with 95:2.5:2.5 EtOAc- HOAC-H2O to give 1.6 g (65%) of the title compound. ¹ H NMR (CDCI3, 300 MHz) δ 2.35 (s, 1H), 4.30 (d, 2H), 4.70 (d, 2H), 6.70-7.85 (m, 17H), 9.5 (s, 2H). MS (DCI/NH3) m/e 481 (M+H+NH ₃ -H ₂ 0) ⁺ , 499 (M+H+NH ₃ ) ⁺ .

Example 25 4-fN-Benzyl-N-(4-phenoxymethvπbenzylaminocarbonvπbenzene-1 .2- dicarboxylic acid The title compound was prepared by the procedures described in Example 24 using the analogous N-benzyl-N-(4-phenoxymethyl)benzylamine compound. The crude compound was chromatographed on silica gel eluting with 95:2.5:2.5 EtOAc-HOAc-H 0 to give 1.5 g (61%) of the title compound as a cream colored amorphous solid. ¹ H NMR (CDCI3, 300 MHz) δ 2.08(d, 1H), 2.25 (q, 1H), 2.35 (s, 1H), 2.50 (t, 1 H), 4.00-5.00 (m, 7H), 6.90-7.80 (m, 17H). MS (DCI/NH ₃ ) m/e 496 (M+H) ⁺ , 513 (M+H+NH ₃ ) ⁺ .

Example 26 5-ff1-f4-Phenoxyphenvπphenethylaminocarbonynbenzene-1.3-dic arboxylic acid A mixture of 4-phenoxyphenyl benzyl ketone (5.5 g, 19 mmol) and benzyl amine (2.04 g, 19 mmol) in methanol (250 mL) was shaken with 5% platinum on carbon (1.1 g) for 16 hours and then for 24 hours under hydrogen at 4 atmospheres. The reaction mixture was filtered and the filtrate evaporated under reduced pressure to give 2-(4-phenoxyphenyl)-1-phenethylamine (7.0 g, 97%) as a colorless solid.

A solution of the above amine (0.54 g, 1.4 mmol) and triethylamine (0.19 mL, 1.4 mmol) in anhydrous methylene chloride was stirred at ambient temperature while a solution of dimethyl 5-chloroformyl-1 ,3-benzene dicarboxylate (0.33 g, 1.3 mmol), prepared by the procedures described in Photochemistry and Photobiology, 51 : 155 (1990), in methylene chloride (15 mL) was added dropwise under a nitrogen atmosphere. After stirring overnight at ambient temperature, the mixture was evaporated under reduced pressure at 20 °C. The solid obtained was chromatographed on silica gel eluting with 8:2 hexane-ethyl acetate to give 442 mg (57%) of 1-[(1-(4- phenoxyphenyl)phenethylaminocarbonyl]-3,5-dimethoxycarbonylb enzene. The diester (360 mg, 6 mmol) was refluxed overnight in a solution of lithium hydroxide (198 mg, 4.8 mmol) in methanol (9 mL) and water (7 mL). The reaction mixture was cooled, diluted with cold water, acidified with dilute

HCI and extracted with ethyl acetate (3 x 40 mL). The combined organic extracts were washed with saturated sodium chloride solution, dried over sodium sulfate and evaporated under reduced pressure to give the title compound as a colorless amorphous solid. ¹ H NMR (CDCI3, 300 MHz) δ 4.40 (d, 2H), 4.75 (d, 1H), 6.78-7.40 (m, 17H), 8.4 (s, 2H). 8.85 (s, 1H). MS (DCI/NH3) m/e 482 (M+H) ⁺ , 499 (M+H+NH ₃ ) ⁺ .

Example 27 4-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1-2- 1 ryitetrazolylbenzoic acid

Example 27A 1 -Methyl-2-cyanoterephthalate 1-Methyl-2-aminoterephthalate (5.15 g, 26.4 mmol) was slurried in 2 N HCI (50 mL), then sodium nitrite (1.60 g, 23.2 mmol) in water (5 mL) was added. The reaction was mechanically stirred for 1 hour, then the pH was adjusted to 8-9 with solid Na2Cθ3. The resulting solution was then added to a solution of CuCN (2.77 g, 30.9 mmol) and NaCN (3.16 g, 64.5 mmol) in water (30 mL). After stirring at room temperature for 3 hours, the reaction mixture was acidified with concentrated HCI (12 mL), and filtered. The solid was dried under vacuum over P2O5 to afford 6.1 g of crude light green solid. The solid was slurried in 9:1 CHC.3-MeOH (140 mL) and filtered. The filtrate was concentrated to give the title compound as a dark yellow solid (3.7 g, 68%) containing -14% (mole) starting aniline ^* . ¹ H NMR (CD3OD) δ 8.42 (s, 1H), 8.35 (d, 1H), 8.26 (d, 1H), 7.84* (d), 7.40 ^* (d), 7.14 ^* (dd), 4.00 (s, 3H), 3.87 ^* (s). MS (DCI/NH3) m/e 223 (M+H+NH ₃ ) ⁺ .

Example 27B Methyl 4-[N-benzyl-N-f4-ρhenoxybenzvπaminocarbonyl1-2-cvanobenzoa te To the compound resulting from Example 27A (320 mg, 1.6 mmol) dissolved in 1 :1 CH2CI2-DMF (14 mL) was added N-benzyl-N-(4- phenoxybenzyl)amine (427 mg, 1.48 mmol), followed by 1-(3- dimethylaminoproρyl)-3-ethylcarbodiimide hydrochloride (341 mg, 1.78 mmol) and 4-(dimethylamino)pyridine (95 mg, 0.08 mmol). After stirring at room

temperature for 2 days, the reaction was diluted with EtOAc, then washed with 2 x 5% citric acid, 2 x saturated NaHCθ3 and brine, dried over Na2S04, filtered and concentrated. The resulting residue was purified by chromatography eluting with 97:3 CHCl3-Et2θ to give 260 mg (40%) of the title compound. H NMR (CDCI ₃ ) δ 8.15 (br d, 1H), 7.87 (s, 1H), 7.73 (d, 1H) 7.37 (m, 6H), 7.28 (m, 2H), 7.13 (m, 2H), 7.00 (m, 4H), 4.70 (br d, 2H), 4.33 (br d, 2H), 4.01 (s, 3H). MS (DCI/NH3) m/e 477 (M+H) ⁺ , 494 (M+H+NH ₃ ) ⁺ .

Example 27C Methyl 4-fN-benzyl-N-(4-phenoxybenzvπaminocarbonyl ^' |-2-

(1 /-fltetrazolylbenzoate To the compound resulting from Example 27B (250 mg, 0.54 mmol) dissolved in DMF (5.4 mL) was added triethylamine hydrochloride (458 mg, 3.33 mmol) and sodium azide (233 mg, 3.58 mmol). The reaction was heated at 110 °C under N2 for 6 hours, then cooled to room temperature and partitioned between EtOAc and 1 M H3PO4. The EtOAc layer was washed with 1 M H3PO4, water and brine, dried over Na2S04, filtered and concentrated. The residue obtained was purified by chromatography eluting with 1 :1 EtOAc- hexane, followed by 98.5:1.5:0.5 CHCl3-MeOH-CH ₃ C0 ₂ H to afford 182 mg (64%) of the title compound which was lyophilized from CHsCN-water to give a white solid. ^" Η NMR (DMSO-d ₆ ) δ 7.95 (br s,1 H), 7.85 (s, 1 H), 7.76 (br d, 1 H) 7.37 (m, 7H), 7.15 (m, 3H), 6.97 (m, 4H), 4.64 (br d, 2H), 4.44 (br d, 2H), 3.72 (s, 3H). MS (DCI/NH3) m/e 520 (M+H) ⁺ , 537 (M+H+NH ₃ ) ⁺ . Anal calcd for C ₃₀ H25N5O4: C, 69.35; H, 4.85; N, 13.48. Found: C, 69.06; H, 4.83; N, 12.84.

Example 27D 4-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl]-2-π - ⁾ tetrazolylbenzoic acid

To the compound resulting from Example 27C (100 mg, 0.21 mmol) dissolved in THF (2.8 mL) and MeOH (1 mL) was added 4 N NaOH (0.2 mL). The reaction mixture was stirred at room temperature overnight, then concentrated and partitioned between EtOAc and 1 M H3PO4. The EtOAc layer was washed with brine, dried over Na2S04, filtered and concentrated to give a residue that was lyophilized from acetonitrile-water to give the title compound

as a white solid (81 mg). ¹ H NMR (DMSO-d ₆ ) δ 8.01 (br m, 1H), 7.78 (d, 1H), 7.77 (s, 1H) 7.35 (m, 7H), 7.15 (m, 3H), 6.97 (m, 4H), 4.64 (br d, 2H), 4.42 (br d, 2H). MS (FAB) m/e 506 (M+H) ⁺ , 504 (M-H) ⁺ . Anal calcd for C29H23N5O4 • 0.5 H ₂ 0: C, 67.70; H, 4.70; N, 13.61. Found: C, 67.43; H, 4.53; N, 13.29.

Example 28 5-| ^" N-Benzyl-N-^4-phenoxybenzvπaminocarbonyl)-2-f1 )tetrazolylbenzoic acid

Example 28A 2-Cvano-5-iodobenzoic acid

The title compound was prepared from 2-amino-5-iodobenzoic acid by the method described in J. Org. Chem. 16: 1275 (1951) in 46% yield. ^" Η NMR (DMSO-de) δ 8.40 (s, 1H), 8.18 (d, 1H), 7.76 (d, 1H). MS (FAB) m/e 274 (M+H) ⁺ , 291 (M-H) ⁺ .

Example 28B 1 -Methyl-6-cvanoisophthalate The compound resulting from Example 28A was treated with trimethylsilyldiazomethane to give the methyl ester. To the ester (155 mg, 0.54 mmol) dissolved in DMF (2 mL) was added water (0.5 mL), followed by anhydrous sodium acetate (172 mg, 2.1 mmol) and palladium(ll)acetate (6 mg, 0.026 mmol). The solution was purged with CO, heated under a CO balloon for 3 hours, cooled to room temperature and partitioned between EtOAc and 1 M H3PO4. The EtOAc layer was washed with brine, dried over Na2S04, filtered and concentrated to give a solid which was partitioned between Et2θ and saturated NaHCθ3. The aqueous layer was acidified and extracted with 3 x Et2θ. The combined organic extracts were dried over MgSθ4, filtered and concentrated to give 83 mg (75%) of the title compound as a tan solid. ¹ H NMR (CD3OD) δ 8.42 (s, 1H), 8.72 (s, 1H), 8.33 (d, 1H), 8.02 (d, 1 H),4.01 (s, 3H). MS (DCI/NH3) m/e 223 (M+H) ⁺ , 240 (M+H+NH ₃ ) ⁺ .

Example 28C Methyl 5-[N-benzyl-N-f4-ρhenoxybenzvπaminocarbonyl]-2-cvanobenzoa te To the compound resulting from Example 28B (80 mg, 0.39 mmol) slurried in CH2CI2 (4 mL) was added oxalyl chloride (57 mg, 0.039 mL, 0.44 mmol) and one drop of DMF. After stirring at room temperature for 45 minutes, the solution was cooled to 0 °C, then treated with a solution of N-benzyl-N-(4- phenoxybenzyl)amine (122 mg, 0.42 mmol) and diisopropylethylamine (118 mg, 0.16 mL, 0.92 mmol) in CH2CI2 (0.5 mL) added dropwise. The cooling bath was removed, and after stirring at room temperature for 3 hours, the same work-up and purification as in Example 27B, except 3:1 hexane-EtOAc was used for the chromatography, to give 50 mg (81%) of the title compound. ¹ H NMR (CDCI3) δ 8.22 (s, 1H), 7.82 (d, 1H), 7.73 (d, 1H), 7.35 (m, 7H), 7.13 (m, 2H), 7.00 (m, 5H), 4.72 (br d, 2H), 4.35 (br d, 2H), 3.98 (s, 3H). MS (DCI/NH3) m/e 477 (M+H) ⁺ , 494 (M+H+NH ₃ ) ⁺ .

Example 28D 5-rN-Benzyl-N-(4-phenoxybenzvπaminocarbonyl]-2-π -Λtetrazolylbenzoic acid Methyl 5-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-2- (1 H)tetrazolylbenzoate was prepared from the compound resulting from Example 28C by the method of Example 27C. MS (ESI) m/e 520 (M+H)+.

The title compound was prepared from the ester by the method of Example 27D. 1H NMR (DMSO-d ₆ ) δ 8.00 (d, 1H), 7.83 (d, 1H), 7.76 (d, 1H) 7.38 (m, 7H), 7.16 (m, 3H), 7.00 (m, 4H), 4.64 (br d, 2H), 4.44 (br d, 2H). MS (DCI/NH3) m/e 506 (M+H) ⁺ , 523 (M+H+NH ₃ ) ⁺ . Anal calcd for C29H23N5O4 ^• 0.65 H ₂ 0: C, 67.34; H, 4.74; N, 13.54. Found: C, 67.39; H, 4.77; N, 13.24.

O 96/34851

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Example 29 4-{rN-Benzyl-N-f4-phenoxybenzyl)aminocarbonyl]amino benzene-1.2- dicarboxylic acid

Example 29A

Dimethyl 4-aminophthalate To dimethyl 4-nitrophthalate (1.15 g, 4.80 mmol) dissolved in MeOH (16 mL) was added ammonium formate (2.9 g, 46.0 mmol). The reaction was purged with N2, then 10 % Pd/C was added. The reaction was heated under reflux for 2.5 hours, cooled to room temperature, filtered through celite and concentrated to give 990 mg (99%) of the title compound as a bright yellow solid. 1H NMR (CDCI ₃ ) δ 7.72 (d, 1H), 6.73 (d, 1H), 6.68 (dd, 1H), 4.18 (br s, 2H), 3.92 (s, 3H), 3.85 (s, 3H). MS (DCI/NH3) m/e 210 (M+H) ⁺ , 227 (M+H+NH ₃ ) ⁺ .

Example 29B Dimethyl 4-{ ^r N-benzyl-N-(4-phenoxybenzyπaminocarbonyllamino)phthal ate The compound resulting from Example 29A (247 mg, 1.20 mmol), the compound resulting from Example 122E (496 mg, 1.40 mmol), and 4- dimethylaminopyridine (41 mg, 0.03 mmol) were dissolved in pyridine (2.5 mL) and heated at 100 °C for 4.5 hours. After cooling to room temperature, the reaction mixture was partitioned between 3 N HCI and EtOAc. The EtOAc layer was washed with brine, dried over Na2S04, filtered and concentrated to give a residue which was purified by chromatography eluting with 3:1 hexane-EtOAC to give 115 mg (18%) of the title compound. ¹ H NMR (CDCI3) δ 7.74 (d, 1H), 7.48 (dd, 1H) 7.45-7.25 (envelope, 10H), 7.13 (m, 1H), 7.02 (m, 4H), 6.56 (s, 1H), 4.62 (s, 4H), 3.88 (s, 3H), 3.85 (s, 3H). MS (DCI/NH3) m/e 542 (M+H+NH ₃ ) ⁺ .

Example 29C 4-{rN-Benzyl-N-^4-phenoxybenzvπaminocarbonvnamino)benzene-1 .2- dicarboxylic acid The title compound was prepared from the compound resulting from

Example 29B by the method of Example 27D. 1H NMR (DMSO-d ₆ ) δ 9.02 (s, 1H), 7.78 (d, 1H), 7.74 (dd, 1H),7.67 (d, 1H), 7.36 (m, 4H), 7.25 (m, 6H), 7.14 (m, 1 H), 6.98 (m, 4H), 4.58 (d, 4H). MS (FAB+) m/e 497 (M+H)+ and (FAB-) m/e 495 (M-H)-. Anal calcd for C29H24N2O6 ^• 0.25 H ₂ 0: C, 69.52; H, 4.93; N, 5.59. Found: C, 69.60; H, 4.89; N, 5.31.

Example 30 4-{[N-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1amino)-2- carboxyphenylacetic acid

Example 30A 4-Nitro-2-carboxyphenylacetic acid Homophthalic acid was nitrated according to the procedure described in J. Org. Chem. 10: 533 (1945) to give the title compound in 52% yield. ¹ H NMR (DMSO-d ₆ ) δ 8.62 (d, 1H), 8.36 (dd, 1H), 7.67 (d, 1H) 4.10 (s, 2H). MS (APCI-) m/e 224 (M-H)".

Example 30B Methyl 4-amino-2-carbomethoxyphenylacetate The compound resulting from Example 30A was treated with trimethylsilyldiazomethane to give the diester which was converted to the aminodiester by the method of Example 29A only refluxing for 1.5 hours. After concentration, the residue was partitioned between EtOAc and water. Isolation of the material in the EtOAc layer gave the title compound. ¹ H NMR (CDCI3) δ 7.34 (d, 1 H), 7.03 (d, 1 H), 6.78 (dd, 1 H), 3.87 (s, 2H), 3.84 (s, 3H), 3.69 (s, 3H). MS (DCI/NH3) m/e 224 (M+H) ⁺ , 241 (M+H+NH ₃ ) ⁺ .

Example 30C 4-{fN-Benzyl-N-te-phenoxybenzvhaminocarbonyl1amino}-2- carboxyphenylacetic acid Methyl 4-{[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]amino}-2- carbomethoxyphenylacetate was prepared from the compound resulting from Example 30B by the method of Example 29B. ^" "H NMR (CDCI ₃ ) δ 7.79 (d, 1H), 7.48 (dd, 1H) 7.42-7.25 (envelope, 8H), 7.13 (m, 3H), 7.02 (m, 4H), 6.41 (s, 1H), 4.64 (d, 4H), 3.95 (s, 2H), 3.85 (s, 3H), 3.68 (s, 3H). MS (DCI/NH3) m/e 539 (M+H) ⁺ , 556 (M+H+NH ₃ ) ⁺ .

4-{[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]amino}-2- carboxyphenylacetic acid was prepared from the above compound by the method of Example 27D, except the reaction had to be heated under reflux for 4 hours. 1H NMR (DMSO-d ₆ ) δ 8.78 (s, 1H), 8.05 (d, 1 H), 7.68 (dd, 1H), 7.36 (m, 4H), 7.27 (m, 5H), 7.17 (d, 1H), 7.10 (m, 1H), 6.98 (m, 4H), 4.58 (d, 4H), 3.82 (s, 2H). MS (ESI-) m/e 509 (M-H)". Anal calcd for C30H26N2O6 ^• 0.65 H ₂ 0: C, 68.99; H, 5.27; N, 5.36. Found: C, 68.94; H, 5.08; N, 5.19.

Example 31 4-rN-Benzyl-N-(4-phenoxybenzv0aminocarbonyπ-2-carboxyphenyl acetic acid

Example 31 A Methyl 4-iodo-2-carbomethoxyphenylacetate To the compound resulting from Example 30B (2.2 g, 10.0 mmol) dissolved in a mixture of 2% HCI (33 mL) and acetone (14 mL) and cooled to -7 °C was added sodium nitrite (745 mg, 10.8 mmol) in water (10 mL) slowly, keeping the reaction temperature <3 °C. The reaction was mechanically stirred for 2 hours, then urea (220 mg, 3.67 mmol) was added, followed by potassium iodide (2.93 g, 17.6 mmol) in water (10 mL). The bath was removed, and the reaction was stirred for 3.5 hours and partitioned between EtOAc and 5% aqueous NaHSθ3. The EtOAc layer was washed with 5% aqueous NaHSθ3 and brine, dried over Na2S04, filtered and concentrated to give a residue which was purified by chromatography eluting with 8:2 hexane-Et2θ to give

1.15 g (34%) of the title compound, which contained -15% (mole) of the des- iodo compound*. -"H NMR (CDCI ₃ ) δ 8.35 (d, 1H), 8.02 ^* (dd), 7.80 (dd, 1H), 7.49* (ddd), 7.37* (ddd), 7.27 ^* (dd), 7.00 (d, 1H), 4.03 ^* (s), 3.95 (s, 2H), 3.88 (s, >3H due to *), 3.61* (s), 3.60 (s, 3H). MS (DCI/NH3) m/e 335 (M+H) ⁺ , 352 (M+H+NH ₃ ) ⁺ .

Example 31 B 4-fN-Benzyl-N-(4-phenoxybenzvflaminocarbonyl]-2-carboxypheny lacetic acid Methyl 4-carboxy-2-carbomethoxyphenylacetate was prepared from the compound resulting from Example 31 A by the method of Example 28B. ¹ H NMR (CD3OD) δ 8.60 (d, 1H), 8.14 (dd, 1H), 7.45 (d, 1H), 4.10 (s, 2H), 3.89 (s, 3H), 3.67 (s, 3H). MS (DCI/NH3) m/e 253 (M+H) ⁺ , 270 (M+H+NH ₃ ) ⁺ .

Methyl 4-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-2- carbomethoxyphenylacetate was prepared from the above compound by the method of Example 28C. ¹ H NMR (CDCI3) δ 8.15 (d, 1 H), 7.60 (dd, 1 H) 7.42- 7.25 (envelope, 8H), 7.13 (m, 3H), 7.00 (m, 4H), 4.70 (br d, 2H), 4.42 (br d, 2H), 4.03 (s, 2H), 3.83 (s, 3H), 3.68 (s', 3H). MS (DCI/NH3) m/e 524 (M+H) ⁺ , 541 (M+H+NH ₃ ) ⁺ .

The title compound was prepared from the above compound by the method of Example 30C. ¹ H NMR (DMSO-d ₆ ) δ 7.95 (d, 1 H), 7.60 (dd, 1 H), 7.30 (m, 8H), 7.15 (m, 3H), 6.98 (m, 4H), 4.55 (br d, 4H), 3.95 (s, 2H). MS (ESI-) m/e 494 (M-H)". Anal calcd for C30H25NO6 ^• 0.50 H2O: C, 71.42; H, 5.19; N, 2.78. Found: C, 71.46; H, 5.04; N, 2.76.

Example 32

4"frN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl]amino)benze ne-1.2-diacetic acid

Example 32A Diethyl 4-nitro-1.2-phenylenediacetate

The title compound was prepared from diethyl 1 ,2-phenylenediacetate by the procedure described in Helv. Chem. Acta, 18: 620 (1935), except the crude product was extracted from the aqueous work-up using Et2θ, and the

residue was purified by chromatography eluting with 8:2 hexane-Et2θ to give the title compound as a light yellow oil in 73% yield. ¹ H NMR (CDCI3) δ 8.17 (d, 1H), 8.11 (dd, 1H), 7.45 (d, 1H), 4.16 (m, 4H), 3.80 (s, 4H), 1.25 (m, 6H). MS (DCI/NH3) m e 296 (M+H) ⁺ , 313 (M+H+NH ₃ ) ⁺ .

Example 32B

4-{[N-Benzyl-N-f4-phenoxybenzyπaminocarbonyl]amino ⁾ benzene-l .2-diacetir: acid Diethyl 4-amino-1 ,2-phenylenediacetate was prepared from the compound resulting from Example 32A by the method of Example 30B. ¹ H NMR (CDCI3) δ 7.03 (d, 1H), 6.60 (d, 1 H), 6.56 (dd, 1H), 4.13 (m, 4H), 3.62 (s, 2H), 3.58 (s, 2H) 1.25 (m, 6H). MS (DCI/NH3) m/e 266 (M+H) ⁺ , 283 (M+H+NH ₃ ) ⁺ .

Diethyl 4-{[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]amino}-1 ,2- phenylenediacetate was prepared from the above compound by the method of Example 29B. ^" Η NMR (CDCI3) δ 7.42-7.25 (envelope, 9H), 7.13 (m, 4H), 7.00 (m, 4H), 6.32 (s, 1H), 4.60 (s, 4H), 4.13 (m, 4H), 3.65 (s, 2H), 3.62 (s, 2H), 1.22 (m, 6H). MS (DCI/NH3) m/e 598 (M+H+NH ₃ ) ⁺ .

The title compound was prepared from the above compound using the method of Example 30C. ¹ H NMR (DMSO-d ₆ ) δ 8.59 (s, 1H), 7.36 (m, 6H), 7.25 (m, 5H), 7.10 (m, 2H), 6.98 (m, 4H), 4.55 (br d, 4H), 3.50 (s, 4H). MS (ESI-) m/e 523 (M-H) ^" . Anal calcd for C31H28N2O6 ^• 0.35 H 0: C, 70.14; H, 5.45; N, 5.28. Found: C, 70.17; H, 5.33; N, 5.12.

Example 33

4-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]benzene-1.2- diacetic acid Diethyl 4-iodo-1,2-phenylenediacetate was prepared from the compound resulting from Example 32B by the method of Example 31 A. The final product contained -15 mole % of the des-iodo compound*. ¹ H NMR (CDCI3) δ 7.52 (s, 1 H), 7.46 (d, 1 H), 7.27 ^* (s) 7.00 (d, 1 H), 4.13 (m, > 4H due to ^* ), 3.71* (s), 3.63 (s, 4H), 1.25 (m, > 6H due to ^* ). MS (DCI/NH3) m e 377 (M+H) ⁺ , 394 (M+H+NH ₃ ) ⁺ .

Diethyl 4-carboxy-1 ,2-phenylenediacetate was prepared from the above compound by the method of Example 28B. ¹ H NMR (CD ₃ OD) δ 7.93 (s, 1 H), 7.88 (d, 1H), 7.36 (d, 1H), 4.13 (m, 4H), 3.78 (s, 4H), 1.23 (m, 6H). MS (DCI/NH3) m/e 295 (M+H) ⁺ , 312 (M+H+NH ₃ ) ⁺ . Diethyl 4-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-1 ,2- phenylenediacetate was prepared from the above compound by the method of Example 28C. ¹ H NMR (CDCI3) δ 7.42-7.20 (envelope, 11 H), 7.13 (m, 3H), 7.00 (m, 4H), 4.70 (br d, 2H), 4.43 (br d, 2H), 4.12 (m, 4H), 3.70 (s, 4H), 1.23 (q, 6H). MS (DCI/NH3) m/e 566 (M+H) ⁺ , 583 (M+H+NH ₃ ) ⁺ . The title compound was prepared from the above compound by the method of Example 30C. ¹ H NMR (DMSO-d ₆ ) δ 7.35 (m, 11H), 7.16 (m, 3H), 6.98 (m, 4H), 4.47 (br d, 4H), 3.64 (s, 2H), 3.62 (s, 2H). MS (ESI-) m/e 508 (M- H)-. Anal calcd for C ₃ ιH ₂ 7N0 ₆ - 0.25 H ₂ 0: C, 72.43; H, 5.39; N, 2.72. Found: C, 72.31 ; H, 5.25; N, 2.68.

Example 34

5-{[N-Benzyl-N-(4-phenoxybenzy0aminocarbonyl1amino}-2- carboxyphenylacetic acid

Example 34A

Methyl 2-bromo-4-nitrobenzoate To 2-bromo-4-nitrotoluene (5.06 g, 23.4 mmol) dissolved in pyridine (23 mL) and water (46 mL) and heated under reflux was added KMnθ4 (18.5 g, 117.0 mmol) in portions over 10 minutes. After heating under reflux overnight, the reaction was filtered through celite (while hot), washed well with hot water, and then cooled to room temperature. This solution was washed two times with Et2θ, and the aqueous layer was acidified, saturated with NaCI, and then extracted three times with EtOAc. The combined EtOAc extracts were concentrated in vacuo to afford 3.8 g of crude material. This carboxylic acid was dissolved in MeOH (30 mL), treated with SOCI2 (1 mL), and then heated under reflux overnight. The reaction mixture was cooled and concentrated, and the residue obtained was crystallized from EtOH (25 mL) to give 3.37 g (55%) of

the title compound. ¹ H NMR (CDCI3) δ 8.53 (d, 1H), 8.23 (dd, 1H), 7.93 (d, 1H), 4.00 (s, 3H).

Example 34B 5-Nitro-2-carboxyphenylacetic acid

The compound resulting from Example 34A (1.52 g, 6.2 mmol) was converted to ethyl 2-(2'-carboxy-4'-nitro)phenylacetoacetate by the method described in Tetrahedron, 31: 2607 (1975) to give 1.83 g (100%) crude material as a brown oil. MS (DCI/NH3) m/e 313 (M+H+NH ₃ ) ⁺ . The above compound (1.83 g, 6.2 mmol) was dissolved in 2 N NaOH (12 mL) and stirred at room temperature for 35 minutes. After acidification with concentrated HCI, the reaction was extracted with EtOAc. The combined organic extracts were washed with brine, dried over Na2S04, then filtered and concentrated to give 1.5 g of tan solid which was recrystallized from water (25 mL) to give 1.01 g (72%) of the title compound. ¹ H NMR (DMSO-d ₆ ) δ 8.29 (d, 1H), 8.22 (dd, 1H), 8.10 (d, 1H), 4.11 (s, 2H).

Example 34C Methyl 5-amino-2-carbomethoxyphenylacetate The compound resulting from Example 34B was treated with trimethylsilyldiazomethane to give the dimethyl ester, which was converted to the title compound by the method of Example 30B, except the compound was purified by chromatography eluting with 6:4 hexane-EtOAc to give 710 mg (72%) of the title compound as a white solid. ^" -H NMR (CDCI3) δ 7.88 (d, 1H), 6.58 (dd, 1 H), 6.49 (d, 1 H), 3.93 (s, 2H), 3.82 (s, 3H), 3.72 (s, 3H). MS (DCI/NH3) m/e 224 (M+H) ⁺ , 241 (M+H+NH3) ^" .

Example 34D Methyl 5-{[N-benzyl-N-f4-phenoxybenzvhaminocarbonvnamino -2- carbomethoxyphenylacetate

To the compound resulting from Example 34C (150 mg, 0.67 mmol) slurried in toluene (1 mL) was added triphosgene (62 mg, 0.21 mmol) and anhydrous K2CO3 (200 mg, 1.45 mmol). The reaction was stirred at 100 °C

under N2 for 2.5 hours, and then N-benzyl-N-(4-phenoxybenzyl)amine (210 mg, 0.73 mmol) was added. After another 3 hours at 100 °C, the reaction was cooled to room temperature and partitioned between water and EtOAc. The EtOAc layer was washed with 4 x 10% citric acid and brine, dried over Na2S04, filtered and concentrated to give a residue which was purified by chromatography eluting with 3:1 hexane-EtOAc to give 123 mg (36%) of the title compound as a white solid. ¹ H NMR (CDCI3) δ 7.93 (d, 1 H), 7.42-7.20 (envelope, 11 H), 7.13 (m, 1H), 7.02 (m, 4H), 6.51 (s, 1H), 4.60 (s, 4H), 3.95 (s, 2H), 3.83 (s, 3H), 3.68 (s, 3H). MS (DCI/NH3) m/e 539 (M+H) ⁺ , 556 (M+H+NH ₃ ) ⁺ .

Example 34E 5-{[N-Benzyl-N-(4-phenoxybenzv aminocarbonvnamino -2- carboxyphenylacetic acid The title compound was prepared from the compound resulting from

Example 34D by the method of Example 30C. ¹ H NMR (DMSO-d ₆ ) δ 8.90 (s, 1H), 7.83 (d, 1H), 7.55 (dd, 1H), 7.45 (d, 1H), 7.35 (m, 4H), 7.26 (m, 5H), 7.12 (m, 1 H), 6.98 (m, 4H), 4.59 (d, 4H), 3.84 (s, 2H). MS (FAB+)m/e 511 (M+H)+ and (FAB-) m/e 509 (M-H)". Anal calcd for C30H26N2O6 ^■ 0.50 H ₂ 0: C, 69.35; H, 5.24; N, 5.39. Found: C, 69.40; H, 5.10; N, 5.19.

Example 35 5-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonvn-2-carboχypheny lacetic acid Methyl 5-iodo-2-carbomethoxyphenylacetate was prepared from the compound described in Example 34C by the method of Example 31 A. H NMR (CDCI3) δ 7.73, 7.72 (both s, total 2H), 7.65 (s, 1H), 3.95 (s, 2H), 3.86 (s, 3H), 3.70 (s, 3H). MS (DCI/NH3) m/e 335 (M+H) ⁺ , 353 (M+H+NH ₃ ) ⁺ .

Methyl 5-carboxy-2-carbomethoxyphenylacetate was prepared from the compound described above by the method of Example 28B. ¹ H NMR (CD3OD) δ 8.05 (d, 1 H), 8.02 (d, 1 H), 7.94 (s, 1 H), 4.05 (s, 2H), 3.85 (s, 3H), 3.65 (s, 3H). MS (DCI/NH3) m/e 253 (M+H) ⁺ , 270 (M+H+NH ₃ ) ⁺ .

Methyl 5-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-2- carbomethoxyphenylacetate was prepared from the compound described

O 96/34851

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above by the method of Example 28C. ¹ H NMR (CDCI3) δ 8.01 (m, 1H), 7.45 (d, 1H) 7.37 (m, 9H), 7.13 (m, 2H), 7.00 (m, 4H), 4.70 (br d, 2H), 4.39 (br d, 2H), 4.00 (s, 2H), 3.87 (s, 3H), 3.68 (s, 3H). MS (DCI/NH3) m/e 524 (M+H) ⁺ , 541 (M+H+NH ₃ ) ⁺ . The title compound was prepared from the compound described above by the method of Example 30C. ^H NMR (DMSO-d ₆ ) δ 7.93 (d, 1H), 7.50-7.25 (envelope, 9H), 7.15 (m, 3H), 7.00 (m, 4H), 4.61 (br d, 2H), 4.40 (br d, 1H), 3.97 (s, 2H). MS (FAB+) m/e 496 (M+H)+ and (FAB-) m/e 494 (M-H)". Anal calcd for C30H25NO6 • 0.35 H ₂ 0: C, 71.80; H, 5.16; N, 2.79. Found: C, 71.87; H, 5.14; N, 2.55.

Example 36 4-([N-Benzyl-N-(4-phenoxybenzyl)aminothiocarbonyl]amino)benz ene-1.2- dicarboxylic acid

Example 36A Dimethyl 4-isothiocvanophthalate To the compound resulting from Example 29A (335 mg, 1.60 mmol) slurried in toluene (1.5 mL) was added thiophosgene (377 mg, 0.25 mL, 3.30 mmol). The reaction mixture was stirred at 75 °C for 5.5 hours, the solution was decanted from insoluble material and concentrated to give 310 mg (77%) of the title compound as a dark brown oil. ¹ H NMR (CDCI3) δ 7.77 (d, 1H), 7.52 (d, 1H), 7.35 (dd, 1H), 3.93 (s, 3H), 3.90 (s, 3H). MS (DCI/NH3) m e 252 (M+H) ⁺ , 269 (M+H+NH ₃ ) ⁺ .

Example 36B Dimethyl 4-{fN-benzyl-N-( ^, 4-phenoxybenzyl ⁾ aminothiocarbonyl|amino)phthalate The compound resulting from Example 36A (290 mg, 1.15 mmol) and N- benzyl-N-(4-phenoxybenzyl)amine (309 mg, 1.07 mmol) were dissolved in toluene (1.2 mL) and heated at 95 °C for 3.5 hours. The reaction was cooled to room tempaerature and partitioned between 10% citric acid and EtOAc. The EtOAc layer was washed with 2 x 10% citric acid and brine, dried over Na2Sθ4, then filtered and concentrated to give a residue which was purified by

chromatography eluting with 3:1 hexane-EtOAc to give 300 mg (52%) of the title compound as light yellow solid. ¹ H NMR (CDCI ₃ ) δ 7.70 (d, 1 H), 7.51 (d, 1H) 7.45-7.25 (envelope, 11H), 7.13 (m, 1H), 7.02 (m, 4H), 5.05 (d, 4H), 3.88 (s, 3H), 3.87 (s, 3H). MS (DCI/NH3) m/e 541 (M+H) ⁺ .

Example 36C 4-{rN-Benzyl-N-C4-phenoxybenzyhaminothiocarbonvnamino}benzen e-1.2- dicarboxylic acid The compound resulting from Example 36B was hydrolyzed by the method of Example 27D. The crude product was purified by chromatography using 95:5:1 CHCI ₃ -MeOH-CH ₃ C0 ₂ H followed by 18:1 :1 EtOAC-water- CH3CO2H. The resultant glass was partitioned between EtOAc and 2 N HCI, then the EtOAc was washed with 2 N HCI and brine, dried over Na2S04, then filtered and concentrated to give a residue which was dissolved and lyophilized as in Example 27D. ¹ H NMR (DMSO-d ₆ ) δ 9.58 (s, 1 H), 8.10 (d, 1 H), 8.03 (s, 1H), 7.57 (d, 1H), 7.30 (m, 9H), 7.14 (m, 1H), 6.98 (m, 4H), 5.05 (d, 4H). MS (FAB-) m e 511 (M-H)". Anal calcd for C29H24N2O5S ^■ 0.75 H ₂ 0): C, 66.21; H, 4.89; N, 5.32. Found: C, 66.23; H, 4.65; N, 5.04.

Example 37

4-ffN-Benzyl-N-(4-phenoxybenzvπaminocarbonyl1methylamino )benzene-1.2- dicarboxylic acid

Example 37A Dimethyl 4-(rN-benzyl-N-f4- phenoxybenzvhaminocarbonyl1methylamino)phthalate The compound resulting from Example 29B (100 mg, 0.19 mmol) was added to suspension of 60% NaH (16 mg of dispersion, 9.6 mg NaH, 0.40 mmol) in DMF (1.7 mL). After a few minutes at room temperature, methyl iodide (80 mg, 0.035 mL, 0.57 mmol) was added. The reaction mixture was stirred at room temperature for 2 hours and then partitioned between EtOAc and 2 N HCI. The EtOAc was washed with brine, dried over Na2S04, then filtered and concentrated to give a residue which was purified by chromatography eluting

with 95:5 CHCl3-EtOAc to give 81 mg (80%) of the title compound as a tacky solid. 1H NMR (CDCI ₃ ) δ 7.70 (d, 1 H), 7.33 (m, 5H), 7.21 (d, 1H), 7.12 (m, 5H), 7.05 (m, 2H), 6.96 (m, 3H), 4.25 (s, 4H), 3.89 (s, 3H), 3.88 (s, 3H), 3.23 (s, 3H). MS (DCI/NH3) m/e 539 (M+H) ⁺ .

Example 37B 4-{fN-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]methylamino ⁾ benzene-1.2- dicarboxylic acid The title compound was prepared from the compound resulting from Example 37A by the method of Example 27D. ¹ H NMR (DMSO-d ₆ ) δ 7.66 (d, 1H), 7.36 (m, 5H), 7.17 (m, 7H), 6.97 (m, 4H), 4.23 (d, 4H), 3.11 (s, 3H). MS (FAB+) m/e 511 (M+H)+ and (FAB-) m/e 509 (M-H)". Anal calcd for C30H26N2O6 ^■ 0.30 H 0: C, 69.84; H, 5.20; N, 5.43. Found: C, 69.81; H, 5.18; N, 5.35.

Example 38 4-frN-Benzyl-N- ⁽ 4-phenoxybenzvπaminocarbonyπoxy ⁾ benzene-1.2- dicarboxylic acid

Example 38A

Dimethyl 4-(fN-benzyl-N- ⁽ 4-phenoxybenzvhaminocarbonvnoxy ⁾ phthalate Sodium hydride (61 mg of 60% dispersion, 37 mg NaH, 1.53 mmol) was slurried in diglyme (2 mL), then with mechanical stirring, dimethyl 4- hydroxyphthalate (280 mg, 1.33 mmol) was added. After 20 minutes, the compound resulting from Example 122E (460 mg, 1.30 mmol) was added, and the reaction mixture was stirred reaction at 100 °C for 1.5 hours. The reaction was cooled to room temperature and partitioned between water and EtOAc. Brine was added to the aqueous layer, which was then extracted with EtOAc. The combined organic extracts were washed with brine, dried over Na2S04, then filtered and concentrated to give a residue which was purified by chromatography eluting with 4:1 hexane-EtOAc to give 500 mg (73%) of the title compound as a tacky solid. 1 H NMR (CDCI3) δ 7.79 (d, 1 H), 7.50 (s, 1 H), 7.41 -

7.20 (envelope, 10H), 7.12 (m, 1H), 7.00 (m, 4H), 4.55 (m, 4H), 3.91 (s, 3H), 3÷90 (s, 3H). MS (DCI/NH3) m/e 526 (M+H) ⁺ , 543 (M+H+NH ₃ ) ⁺ .

Example 38B 4-{[N-Benzyl-N-(4-phenoxybenzyπaminocarbonvπoxy)benzene-1. 2- dicarboxylic acid The title compound was prepared from the compound resulting from Example 38A by the method of Example 36C. 1H NMR (DMSO-d ₆ ) δ 7.66 (d, 1 H), 7.38 (m, 11 H), 7.14 (m, 1 H), 7.00 (m, 4H), 4.63 (br d, 2H), 4.52 (br d, 2H). MS (FAB-) m/e 496 (M-H)". Anal calcd for C29H23NO7 ^■ 0.50 H ₂ 0: C, 68.77; H, 4.78; N, 2.77. Found: C, 68.90; H, 4.51; N, 2.60.

Example 39 4-(rN-Benzyl-N-(4-phenoxybenzyπaminocarbonvπmethyl .2-benzene dicarboxylic acid

Example 39A Dimethyl 4-bromomethylphthalate To a solution of dimethyl 4-methylphthalate (3.15 g, 15.1 mmol) dissolved CCI4 (60 mL) was added N-bromosuccinimide (2.74 g, 15.4 mmol) and 2,2'-azobisisobutyronitrile (122 mg, 0.9 mmol). The reaction was heated under reflux for 1 day, cooled to room temperature, and then chilled at 5 °C for 1.5 hours before filtering through celite. The filtrate was concentrated to 4.4 g of crude oil, which by ¹ H NMR (CDCI3) was approximately 65 mole % title compound (s, 4.50 ppm, ArCH2Br), 18 mole % dibromide (s, 6.63 ppm, ArCHBr2), and 17 mole % starting material (s, 2.42 ppm, ArCHβ).

Example 39B Dimethyl 4-{2'-ftris(benzotriazol-1 -vMethvPphthalate The title compound was prepared from tris(benzotriazol-1-yl)methane

(1.1 g, 3.0 mmol) and the compound resulting from Example 39A (1.0 g, which contained -650 mg, 2.3 mmol bromomethyl compound) by the method described in Synthesis, 666 (1990); this is the reference for the synthesis of

tris(benzotriazol-1-yl)methane as well. The crude material was purified by chromatography eluting with 6:4 hexane-EtOAc to give 500 mg (38%) of the product as a brown glass. ¹ H NMR (CDCI ₃ ) δ 8.09 (m, 3H), 7.41-7.20 (envelope, 8H), 6.95 (m, 4H), 5.44 (s, 2H), 3.81 (s, 3H), 3.73 (s, 3H). MS (DCI/NH3) m/e 591 (M+H+NH ₃ ) ⁺ .

Example 39C Dimethyl 4-carboxymethylphthalate The title compound was prepared from the compound resulting from Example 39B (492 mg, 0.86 mmol) by the method described in Synthesis, 666 (1990). The crude material was purified by chromatography eluting with 6:4 hexane-EtOAc followed by 98.5:1.5:0.5 CHCl3-MeOH-CH3Cθ2H to give 105 mg (48%) of the title compound as a brown solid. H NMR (CD3OD) δ 7.70 (d, 1 H), 7.63 (d, 1 H), 7.52 (dd, 1H), 3.86 (s, 3H), 3.85 (s, 3H), 3.70 (s, 2H). MS (DCI/NH3) m/e 253 (M+H) ⁺ , 270 (M+H+NH ₃ ) ⁺ .

Example 39D Dimethyl 4-{fN-benzyl-N-(4-phenoxybenzyπaminocarbonvnmethyl)phthalat e The title compound was prepared from the compound resulting from Example 39C by the method of Example 28C. ¹ H NMR (CDCI3) δ 7.70 (m, 1 H), 7.56 (m, 1H), 7.47-7.28 (envelope, 6H), 7.23-6.92 (envelope, 9H), 4.63 (d, 2H), 4.43 (d, 2H), 3.90, 3.88 (both s, total 6H), 3.83 (d, 2H). MS (DCI/NH3) m/e 524 (M+H) ⁺ , 541 (M+H+NH ₃ ) ⁺ .

Example 39E

4-ffN-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl1methyl)benz ene-1.P- dicarboxylic acid The title compound was prepared from the compound resulting from Example 39D by the method of Example 27D. ¹ H NMR (DMSO-d ₆ ) δ 7.61 (m, 1 H), 7.53 (m, 1 H), 7.45-7.10 (envelope, 11 H), 6.97 (m, 4H), 4.61 (d, 2H), 4.50 (d, 2H), 3.93 (d, 2H). MS (FAB+) m/e 496 (M+H)+ and (FAB-) m/e 494 (M-H)". Anal calcd for C30H25 O6 -0.50 H ₂ 0: C, 71.42; H, 5.19; N, 2.78. Found: C, 71.30; H, 5.06; N, 2.62.

Example 40 4-fN-Benzyl-N-(4-phenoxybenzvπaminothiocarbonyπbenzene-1.2 -dicarboxylic acid

Example 40A Dimethyl 4-rN-benzyl-N- -phenoxybenzvπaminothiocarbonyriphthalate To the compound resulting from the first two sentences of Example 9 (200 mg, 0.40 mmol) dissolved in THF (0.8 mL) was added Lawesson's reagent (225 mg, 0.56 mmol). The reaction was stirred at room temperature for 4 days arid then concentrated to give a residue which was purified by chromatography eluting with 85:15 hexane-EtOAc to give 100 mg (48%) of the title compound as a yellow solid. ^" -H NMR (CDCI ₃ ) δ 7.67 (m, 2H), 7.49 (m, 1 H) 7.37 (m, 7H), 7.13 (m, 1H), 7.02 (m, 6H), 5.39 (v br s, 2H), 4.55 (d, 2H), 3.90, 3.89, 3.88 (all s, total 6H). MS (DCI/NH3) m/e 526 (M+H) ⁺ , 543 (M+H+NH3) ^" .

Example 40B 4-fN-Benzyl-N-(4-phenoxybenzyl)aminothiocarbonyl]benzene-1.2 -dicarboxylic acid The title compound was prepared from the compound resulting from

Example 40A by the method of Example 27D. ¹ H NMR (DMSO-d ₆ ) δ 7.72 (m, 1H), 7.60 (m, 1H), 7.57-7.30 (envelope, 8H), 7.20-6.90 (envelope, 7H), 5.41 (d, 2H), 4.63 (d, 2H). MS (FAB+) m/e 498 (M+H)+ and (FAB-) m/e 496 (M-H)". Anal calcd for C29H ₂ 3N0 ₅ S - 1.20 H ₂ 0: C, 67.09; H, 4.93; N, 2.70. Found: C, 67.03; H, 4.63; N, 2.90.

Example 41 5-fN-Benzyl-N-(2-phenoxybenzv0aminocarbonyl]benzene-1.2.4-tr icarboxylic acid ,N-Benzyl-N-(2-phenoxybenzyI) amine was prepared using the same procedure of Example 1A replacing the 4-phenoxybenzaldehyde with 2- phenoxybenzaldehyde.

The title compound was prepared by the procedures described in Example 4 using N-benzyl-N-(2-phenoxybenzyl) amine in place of N-benzyl-N- (4-phenoxybenzyl)amine. H NMR (DMSO-d ₆ , 300 MHz) δ 4.15 (d, 4H), 6.60- 7.50 (m, 14H), 7.60 (d,1 H) , 8.25 (d,1 H). MS (FAB)+ m/e 526 (M+H)+ and (FAB)" m/e 524 (M-H)-.

Example 42

5-rN-Benzyl-N-f3-phenoxybenzvhaminocarbonyl1benzene-1.2.4 -tricarboxylic acid N-Benzyl-N-(3-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the 4-phenoxybenzaldehyde with 3- phenoxybenzaldehyde.

The title compound was prepared by the procedures described in Example 4 using N-benzyl-N-(3-phenoxybenzyl) amine in place of N-benzyl-N- (4-phenoxybenzyl)amine. ^" Η NMR (DMSO-d ₆ , 300 MHz) δ 4.20 (d, 4H), 6.75- 7.40 (m, 14H), 7.60 (d, 1H), 8.15 (d,1H). MS (FAB)+ m/e 526 (M+H)+ and (FAB)" m/e 524 (M-H)-.

Example 43 5-fN-Benzyl-N-f4-phenylbenzvπaminocarbonyl1benzene-1.2.4-tr icarboxylic acid N-Benzyl-N-(4-phenylbenzyl)amine was prepared using the same procedure of Example 1A replacing the 4-phenoxybenzaldehyde with 4- phenylbenzaldehyde. The title compound was prepared by the procedures described in

Example 4 using N-benzyl-N-(4-phenylbenzyl)amine in place of N-benzyl-N-(4- phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 4.20 (s, 4H), 7.20 -7.78 (m, 15H), 8.25 (s, 1H). MS (FAB)+ m/e 510 (M+H) ⁺ and (FAB)" m e 508 (M-H)-.

Example 44 5-fN-Cvclohexylmethyl-N-f4-phenoxybenzvπaminocarbonvnbenzen e-1.2.4- tricarboxylic acid N-Cyclohexylmethyl-N-(4-phenoxybenzyl)amine was prepared using the same procedure of Example 1A replacing the benzylamine with cyclohexylmethylamine.

The title compound was prepared by the procedures described in Example 4 using N-cyclohexyl-N-(4-phenoxybenzyl)amine in place of N- benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 0.80 -1.80 (m, 8H), 2.40-2.80 (m, 2H), 4.00 (m, 2H), 4.3 (s,2H), 6.90 -7.60 (m, 10H), 8.25 (d, 1H). MS (FAB)+ m/e 610 (M+H) ⁺ and (FAB) ^" m/e 608 (M-H)-.

Example 45 5-[N-(3.4-Difluorobenzvn-N-f4-phenoxybenzvnaminocarbonyl benzene-1.2.4- tricarboxylic acid N-(3,4-Difluorobenzyl)-N-(4-phenoxybenzyl)amine was prepared using the same procedure of Example 1A replacing the benzylamine with 3,4- difluorbenzyl amine. The title compound was prepared by the procedures described in

Example 4 using N-(3,4 difluorobenzyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ^" Η NMR (DMSO-d ₆ , 300 MHz) δ 4.20 (s, 2H), 4.6 (m, 2H), 6.80-7.25 (m, 12H), 7.65 (d, 1H), 8.25 (d,1H). MS (FAB)+ m/e 562 (M+H)+ and (FAB)" m/e 560 (M-H)-.

Example 46 5-fN-f3-TriluoromethylbenzylVN-f4-phenoxybenzyhaminocarbonvn benzene-

1.2.4-tricarboxylic acid N-(3-Trifluoromethylbenzyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 3- trifluormethylbenzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(3- trifluoromethylbenzyl)-N-(4-phenoxybenzyl)amine in

place of N-benzyl-N-(4-phenoxybenzyl)amine. ^" Η NMR (DMSO-d ₆ , 300 MHz) δ 4.25 (s, 2H), 4.40 (s, 2H), 6.80 -7.80 (m, 14H), 8.30-(d, 1H). MS (FAB)+ m/e 594 (M+H)+ and (FAB)" m/e 592 (M-H)-.

Example 47

5-fN-f2-Triluoromethylbenzvn-N-f4-phenoxybenzvhaminocarbo nyl]benzene-

1.2.4-tricarboxylic acid N-(2-Trifluoromethylbenzyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 2- trifluormethylbenzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- trifluoromethylbenzyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d6, 300 MHz) δ 4.25 (s, 2H), 4.40 (s, 2H), 6.80 -7.95 (m, 13H),8.15 (s,1H), 8.80 (d, 1H). MS (FAB)+ m/e 594 (M+H)+ and (FAB)" m/e 592 (M-H)-.

Example 48 5-fN-f2-Methoxybenzvπ-N-f4-phenoxybenzyπaminocarbonvnbenze ne-1.2.4- tricarboxylic acid N-(2-Methoxybenzyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 2- methoxybenzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- methoxylbenzyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d _6l 300 MHz) δ 3.60 (s, 3H), 4.20 (d, 2H), 4.60 (s, 2H), 6.80 - 7.25 (m, 13H), 7.90 (s, 1H), 8.45 (s, 1 H). MS (FAB)+ m/e 556 (M+H)+ and (FAB)- m/e 554 (M-H)-.

Example 49 5-rN-f2-Methylbenzvπ-N-f4-phenoxybenzyl)aminocarbonvnbenzen e-1.2.4- tricarboxylic acid N-(2-Methylbenzyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 2-methylbenzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- methyllbenzyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. H NMR (DMSO-dβ, 300 MHz) δ 1.95 (s , 3H), 4.20 (d, 2H), 4.75 (s, 2H), 6.80 -7.50 (m, 13H), 7.50 (d, 1 H), 8.15, (d, 1H). MS (FAB)+ m/e 610 (M+H)+ and (FAB)- m/e 608 (M-H)-.

Example 50 5-rN- 4-Nitrobenzvπ-N-(4-ρhenoxybenzvπaminocarbonvπbenzene-1.2 .4- tricarboxylic acid N-(4-Nitrobenzyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 4-nitrobenzyl amine. The title compound was prepared by the procedures described in

Example 4 using N-(4-nitrobenzyl)-N-(4-phenoxybenzyl)amine in place of N- benzyl-N-(4-phenoxybenzyl)amine. ^" <H NMR (DMSO-d ₆ , 300 MHz) δ 4.25 (d, 2H), 4.70 (m, 2H), 6.80 -7.80 (m, 12H), 8.00-8.20 (m, 3H). MS (FAB)+ m/e 571 (M+H)+ and (FAB)" m/e 569 (M-H)-.

Example 51 5-rN-fNaphth-1-ylmethylVN-f4-phenoxybeπzyl)aminocarbonyl1be nzene-1.2.4- tricarboxylic acid N-(Naphth-1-ylmethyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with naphth-1- ylmethylamine.

The title compound was prepared by the procedures described in Example 4 using N-(Napth-1-ylmethyl)-N-(4-phenoxybenzyl)amine in place of

N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 4.20 (s, 1H), 4.60 (s, 1H), 5.10 (s, 2H), 6.65 -8.20 (m, 18H). MS (FAB)+ m/e 576 (M+H)+ and (FAB)- m e 574 (M-H)-.

Example 52

S-fN-tø-Phenylpropyl^N- -phenoxybenzvhaminocarbonvπbenzene-l ,?^- tricarboxylic acid N-(3-PhenylpropyI)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 3- phenylpropylamine.

The title compound was prepared by the procedures described in Example 4 using N-(3-phenylpropyl)-N-(4-phenoxybenzyl)amine in place of N- benzyl-N-(4-phenoxybenzyI)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 1.60-1.90 (m, 2H), 2.80 (m, 2H), 4..25 (s, 2H), 4.75 (m, 2H) 6.90 -7.45 (m, 14H), 7.60 (d, 1 H), 8.25 (d, 1H). MS (FAB)+ m/e 554 (M+H)+ and (FAB)- m/e 552 (M-H)-.

Example 53

5-rN-(2-Thienylmethyπ-N-f4-phenoxybenzvπaminocarbonyl1b enzene-1.2.4- tricarboxylic acid N-(2-Thienylmethyl)-N-(4-phenoxybenzyl) amine was prepared using the same procedure of Example 1A replacing the benzylamine with 2- thienylmethylamine.

The title compound was prepared by the procedures described in Example 4 using N-(2-thienylmethyl)-N-(4-phenoxybenzyl)amine in place of N- benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-dβ, 300 MHz) δ 4.20 (s, 2H), 4.80 (s, 2H), 6.80 -7.25 (m, 12H), 8.00 (m, 1H), 8.60 (m, 1H). MS (FAB)+ m/e 532 (M+H)+ and (FAB)" m/e 530 (M-H)-.

Example 54 5-fN-Benzyl-N- 4-phenoxybenzvπaminocarbonyπ-4-methoxycarbonyl- benzene-1.2-dicarboxylic acid To a solution of the compound resulting from Example 72A (0.25 g, 0.9 mmol) and Et3N (0.125 mL, 0.9 mmol) in 15 mL of THF and cooled to 0 °C was added a solution of N-benzyl-N-(4-phenoxybenzyl)amine (0.25 g, 0.9 mmol) in 5 mL THF slowly over 5 minutes. The reaction mixture was stirred for 1 hour at 0 °C before adding a solution of MeOH (0.034 mL, 0.9 mmol), Et^N (0.125 mL, 0.9 mmol) contained in 5 mL of THF. The reaction was then stirred for another hour at 0 °C before evaporation. To the residue was added 5 mL of CH2CI2, 5 mL of THF, and 15 mL of 10% HCI, and the mixture was stirred overnight at room temperature. The volatiles were removed under reduced pressure, H2O was added and the mixture was extracted with EtOAc. The combined organic extracts were washed with saturated NaCI and evaporated. (0.4 g). Flash silica gel chromatography eluting with 95:4:1 CHCl3-MeOH-HOAc yielded the title compound. 1H NMR (DMSO-d ₆ , 300 MHz) δ 3.80 (m, 3H), 4.20 (d, 2H), 4.80 (m, 2H), 6.80 - 7.45 (m, 14H), 7.60 (s,1H), 8.00 (s, 1H). MS (FAB)+ m/e 540 (M+H)+ and (FAB)" m/e 538 (M-H)"-

Example 55

5-fN-Benzyl-N-f4-phenoxybenzyl)aminocarbonyl1-1.2-dimetho xycarbonyl- benzene-4-carboxylic acid To a solution containing the compound resulting from Example 72A (0.34 g, 1.3 mmol) in 15 mL of THF cooled to -30 ^C C was added a solution of Et ₃ N (0.36 mL, 2.6 mmol) and MeOH (0.104 mL, 2.6 mmol) in THF (15 mL) over 5 minutes. The reaction mixture was stirred at -30 °C for 45 minutes, and then a solution of N-benzyl-N-(4-phenoxybenzyl) amine (0.38 g, 1.3 mmol) and Et ₃ N (0.18 mL, 2.6 mmol) in THF (10 mL) was added. The reaction was stirred at room temperature overnight and then evaporated and taken up in EtOAc. The reaction mixture was washed with 5% HCI followed by saturated NaCI solution and evaporated to give 0.5 g of crude product. Flash silica gel chromatography eluting with 95:4:1 CHCI ₃ -MeOH-HOAc yielded the title compound. ¹ H NMR

(DMSO-de, 300 MHz) δ 3.65 (s, 3H), 3.75 (s, 3H), 4.20 (m, 2H), 4.60 (s, 2H), 6.80 -7.60 (m, 12H), 7.90 s,1 H), 8.50 (s,1H). MS (FAB)- m/e 552 (M-H)-.

Example 56 5-[N-Benzyl-N-^4-phenoxybenzvhaminocarbonyπ-2-methoxycarbon yl- benzene-1.4-dicarboxylic acid To a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.5 g, 2.3 mmol) in 20 mL of acetone cooled in a salt-ice bath was added a solution of N- benzyl-N-(4-phenoxybenzyl)amine (0.66 g, 2.3 mmol) and diisopropylethyl amine (0.3 g , 2.3 mmol) in 10 mL of acetone dropwise over 2 hours via syringe pump. After stirring an additional hour, the reaction mixture was evaporated under reduced pressure at room temperature. Methanol was added, and then the reaction mixture was refluxed overnight. The volatiles were removed under reduced pressure, and then 5% HCI was added and the mixture extracted with EtOAc. The combined organic extracts were washed with saturated NaCI solution, and then evaporated to give a foam. Flash silica gel chromatography eluting with 95:4:1 CHCI ₃ -MeOH-HOAc yielded of 0.042 g of the title compound. ^" >H NMR (DMSO-d ₆₍ 300 MHz) δ 3.80 (d, 3H), 4.20 (m, 2H), 4.6 (m,2H), 6.80 -8.10 (m, 16H). MS (FAB)+ m/e 540 (M+H)+ and (FAB)" m/e 538 (M-H)".

Example 57 5-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonyl -1-methoxycarbonyl- benzene-2.4-dicarboxylic acid The title compound is isolated from the column chromatography described in Example 56 as another fraction.

Example 58 5-rN-Benzyl-N-C4-phenoxybenzvπaminocarbonyl]-1.4-dimethoxyc arbonyl- benzene-2-carboxylic acid A solution of 1 ,4-dimethylcarboxylate benzene-2,5-dicarbonyl chloride, prepared according to Kogyo Kagaku Zaashi, 71: 1559, (1968), (0.125 g, 0.4 mmol), N-benzyl-N-(4-phenoxybenzyl)amine (0.06 g, 0.4 mmol), NaOH (0.4 mL, 1 N) and CH2CI2 (20 mL) was stirred at room temperature for 2.5 hours. The volatiles were removed under reduced pressure and then water was added. The aqueous solution was washed with ether and then acidified and extracted with EtOAc. The combined organic extracts were evaporated and the residue obtained (0.092 g) flash silica gel chromatographed eluting with 95:4:1 CHCI3- MeOH-HOAc to yield the title compound. ¹ H NMR (CDCI3 , 300 MHz) δ 3.90 (dd, 6H), 4.20 (d, 2H), 4.75 (m, 2H), 6.90 -7.40 (m, 14H), 7.6 (d,1H), 8.60 (d, 1H). MS (FAB)+ m/e 554 (M+H)+ and (FAB)" m/e 552 (M-H)-.

Example 59 5-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-2.4-dimethoxyc arbonyl- benzene-1 -carboxylic acid The title compound is prepared by the procedures described in Example 58 except using 1 ,5-dimethylcarboxylate benzene-2,4-dicarbonyl chloride, prepared according to Kogyo Kagaku Zaashi, 71 : 1559, (1968), instead of 1,4- dimethylcarboxylate benzene-2,5-dicarbonyl chloride.

Example 60 5-fN-Benzyl-N-(4-phenoxybenzv0aminocarbonyri-1.2.4- trimethoxycarbonbenzene A solution of 5-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl] benzene- 1 ,2,4-tricarboxylic acid (0.025 g, 0.074 mmol), MeOH (8 μL, 2 mmol), acetone (2 mL), and CH2CI2 (1 mL) was cooled to 0 °C. To this solution was added Me3SiCH2N2( 2 M in hexane, 0.095 mL) over 5 minutes. The reaction was then stirred for 30 minutes and evaporated to afford the title compound as an amorphous solid (20 mg). MS (FAB)+ m e 568 (M+H)+.

Example 61 5-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1-1.2-benzenedic arboxylic dianhvdride-4-carboxylic acid sodium salt To a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.5 g, 2.3 mmol) in 20 mL of acetone cooled in a salt-ice bath was added a solution of N- benzyl-N-(4-phenoxybenzyl)amine (0.66 g, 2.3 mmol) and diisopropylethylamine (0.3 g , 2.3 mmol) in 10 mL of acetone dropwise over 2 hours via syringe pump. After stirring an additional hour, the reaction mixture was evaporated under reduced pressure at room temperature, treated with 5% HCI and extracted with EtOAc. The combined organic extracts were washed with saturated NaCI solution and then evaporated to give a foam. This was then taken up in cold methanol (2 mL) and treated with a solution of NaOH (1 molar equivalent), stirred for 5 minutes and evaporated to afford a solid. ¹ H NMR (D ₂ 0, 300 MHz) δ 3.80 (m, 2H), 4.20 - 4.90 (m, 4H), 7.20 -8.10 (m, 14H). MS m/e 552 (M+Na)+.

Example 62 4-fN-Benzyl-N-(4-phenoxybenzyπaminocarbonyr)-1.2- dimethoxycarbonylbenzene To a solution of 4-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl] benzene-1 ,2-dicarboxylic acid (0.1 g, 0.2 mmol), MeOH (0.015 mL, 0.4 mmol), acetone (5 mL) and CH2CI2 (1 mL) cooled to 0 °C was added Me3SiCH2N2 (2 M in hexane) (0.2 mL) over 5 minutes. The reaction was then stirred for 30 minutes and evaporated to give 4.8 mg of the title compound as an amorphous solid. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.80 (dd, 6H), 4.40 (m, 2H), 4.60 (m, 2H), 6.90 -8.20 (m, 17H). MS (FAB)+ m/e 510 (M+H) ⁺ .

Example 63 2-[N-Benzyl-N-f4-Dhenoxybenzvπaminocarbonylmethyl]benzoic acid To a solution of homophthalic anhydride (0.81 g, 5 mmol), diisopropylethyl amine (0.87 mL, 5 mmol) and THF (20 mL) cooled to 0 °C was added a solution of N-benzyl-N-(4-phenoxybenzyl)amine (1.44 g, 5 mmol) in 10 mL THF slowly over 2 hours. The reaction was allowed to come to room

temperature overnight and then evaporated. To the residue was added 10% HCI, and the solution was extracted with EtOAc. The combined organic extracts were washed with saturated NaCI solution, dried (MgSθ4) and evaporated. The oil was then crystallized from hot CH3CN to give the title compound as a white solid (0.51 g). ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 4.20 (d, 2H), 4.45 (d, 2H) 4.60 (d, 2H), 6.90 -7.50 (m, 16H) 7.95 (dd, 2H). MS (FAB)+ m/e 452 (M+H)+.

Example 64 5-[N-Benzyl-N-f4-phenoxybenzyπaminocarbonyl ^' |-4-methoxybenzene-1- sulfonamide

A solution of 4-methoxy-1-aminosulfonylbenzene-3-carboxylic acid (0.232 g, 1 mmol), N-benzyl-N-(4-phenoxybenzyl)amine (0.289 g, 1 mmol), N- hydroxybenzotriazole-H2θ (0.135 g, 1 mmol), and dicyclohexyl- carbodiimide (0.191 g, 1 mmol) in 20 mL THF was stirred at room temperature for 72 hours. The volatiles were removed under reduced pressure, water was added to the residue, and the resulting solution was extracted with CH2CI2. The combined organic extracts were washed with 10 % HCI, dried ( MgSθ4) and evaporated. The crude product was crystalized from acetonitrile and then further purified by flash column chromatography on silica gel eluting with 95:4:1 CHCI ₃ -MeOH-HOAc to afford 0.067 g of the title compound. ^" -H NMR (DMSO- de, 300 MHz) δ 3.90 (d, 2H), 4.20 - 4.50 (m, 3H), 4.80 (m, 1H), 6.90 -7.40 (m, 15H), 7.80 (m, 2H). MS (FAB)+ m/e 503 (M+H) ⁺ .

Example 65 4- N-Benzyl-N-f4-phenoxybenzvπaminocarbonylmethvnbenzoic acid

To a solution of terephthaloyl chloride (1.06 g, 5.2 mmol) and Et3N (0.75 g, 7.8 mmol) in 20 mL of THF cooled to 0 °C was added N-benzyl-N-(4- phenoxybenzyl)amine (2.25 g, 7.8 mmol) in 10 mL THF slowly over 15 minutes. The reaction was then stirred overnight. The volatiles were removed under reduced pressure, 10% NaOH solution was added and the solution was washed with ethyl ether. The aqueous phase was then acidified with 10 % HCI and extracted with EtOAc. The combined organic extracts were washed with saturated NaCI solution, dried and evaporated. The crude product was purified

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by flash silica gel chromatography eluting with 95:4:1 CHC -MeOH-HOAc to give 0.039 g of the title compound. H NMR (DMSO-d ₆ , 300 MHz) δ 4.40 (d, 2H), 4.60 (d, 2H), 6.90 -7.50 (m, 14H), 7.60 (d, 2H), 8.00 (d, 2H). MS (FAB)+ m/e 438 (M+H)+.

Example 66 1-rN-Benzyl-N-r4-phenoxybenzyl)aminocarbonylmethyl]biphenyl- 1 '-carboxylic acid A solution of diphenic anhydride (1.12 g. 5 mmol), N-benzyl-N-(4- phenoxybenzyl)amine (1.44 g, 0.005 mol ) and triethylamine (0.5 g, 5 mmol) in 50 mL of toluene was refluxed for 2 hours and then left at room temperature overnight. The toluene was evaporated, ethyl acetate was added to the residue and the resulting solution was washed with saturated NaCI solution, dried and evaporated. Recrystallization of the oil from hot acetonitrile yielded 0.665 g of the title compound as a white solid. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.60 (m, 2H), 4.60 - 5.10 (m, 2H), 6.50 -7.60 (m, 20H), 7.9 (m, 2H). MS (FAB)+ m/e 514 (M+H)+ and (FAB)- m/e 512 (M-H)-.

Example 67 5-fN-Benzyl-N-fL-methionine ethyl ester)aminocarbonylmethyl]benzene-1.2.4- tricarboxylic acid N-Benzyl-L-methionine ethyl ester was prepared using the same procedure as given for the preparation for N-benzyl-N-(4- phenoxybenzyl)amine except replacing the 4-phenoxybenzylamine with L- methionine ethyl ester.

The title compound was prepared by the procedures described in Example 4 using N-benzyl-N-(L-methionine ethyl ester)amine in place of N- benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 1.30 (dt, 3H), 1.60 -2.60 (m, 8H), 3.20 (m, 2H), 4.20-4.40 (m, 2H), 7.20 -8.40 (m, 7H). MS (FAB)+ m/e 504 (M+H) ⁺ and (FAB)- m/e 502 (M-H)-.

Example 68 5-fN-Benzyl-N-fL-methionine methyl esteήaminocarbonylmethyllbenzene-

1 ,2.4-tricarboxylic acid N-Benzyl-N-(L-methionine methyl ester) amine was prepared using the same procedure as given for the preparation for N-Benzyl-N-(4- phenoxybenzyl) amine except replacing the 4-phenoxybenzyl amine with L- methionine methyl ester amine.

The title compound was prepared by the procedures described in Example 4 substituting N-benzyl-N-(L-methionine methyl ester)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-dβ, 300 MHz) δ 1.20 (dt, 3H), 1.60 - 2.60 (m, 8H), 3.20 (m, 2H), 4.20 - 4.40 (m, 2H), 7.20 - 8.40 (m, 7H). MS (FAB)+ m/e 490 (M+H)+ and (FAB)" m/e 488 (M-H)-.

Example 69 acid To a solution of 5-[N-benzyl-N-(L-methionine methyl ester)aminocarbonyl]benzene-1,2,4-tricarboxylic acid (0.160 g, 0.3 mmol) in THF (9 mL) and water (1 mL) was added lithium hydroxide monohydrate (0.130 g, 3.3 mmol). The reaction was then heated for 2 hours and then evaporated to remove volatiles. The solution was acidified and then extracted with ethyl acetate. The combined organic extracts were washed with saturated NaCI solution and evaporated to yield a solid (0.075 g). ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 1.40 - 2.60 (m, 8H), 3.60 (m, 2H), 4.10 - 4.20 (m, 2H), 7.20 - 8.20 (m, 7H). MS (FAB)+ m e 476 (M+H)+ and (FAB)- m/e 474 (M-H)-.

Example 70

5-[N-Benzyl-N-fsιπ-f4-acetoxy-5-methyl-6- phenylhexyhaminocarbonylmethvnbenzene-l .2.4-tricarboxylic acid

Example 70A syn -M-Methyl-2-hvdroxy)-5-benzyloxyρentylphenyl ketone TiCU (1.0 M solution in CH2CI2, 16.8 mL) was added dropwise to a -78 °C solution of propiophenone (2.05 g, 15.2 mmol) in 77 mL CH2CI2. After 5 minutes at -78 °C, EtβN (2.3 mL, 16.8 mmol) was added, and the reaction mixture was stirred at -78 °C for 0.5 hours. 4-Benzyloxybutyraldehyde (3.0 g, 16.8 mmol), prepared by the method described in Heterocycles 28(2): 663, (1989), was added dropwise, neat. The reaction mixture was stirred for 0.5 hours at -78 °C and then was quenched by the addition of 50% saturated NH4CI solution. The solution was warmed to room temperature and extracted with CH2CI2. The combined organic extracts were washed with saturated NaCI solution, dried (MgS04), filtered, concentrated, and flash chromatographed on silica gel eluting with 85:15 hexane-ethyl acetate to afford the title compound (3.67 g) as a clear oil. ¹ H NMR (300 MHz, CDCI3) δ 1.28 (d, 3H), 1.60 (t,3H), 1.67-1.88 (m, 2H), 3.52 (m, 3H), 4.03 (m, 1H), 4.51 (s, 2H), 7.32 (s, 5H), 7.48 (t, 2H), 7.59 (t, 2H), 7.95 (d, 2H). MS (DCI/NH3) m/e 313 (M+H)+.

Example 70B syn -C1-Methyl-2-acetoxy)-5-benzyloxypentylphenyl ketone Acetic anhydride (1.1 mL, 11.7 mmol) was added dropwise to a 0 °C solution of the compound resulting from Example 70A and a catalytic amount of DMAP in 100 mL CH2CI2. The reaction mixture was stirred for 24 hours at room temperature, then 0.1 N HCI was added. The mixture was extracted with CH2CI2 (3x). The combined organic layers were washed with saturated NaCI solution, dried (MgS04), filtered, and concentrated to afford the title compound (2.9 g) as a colorless oil. ¹ H NMR (300 MHz, CDCI3) δ 1.21 (d, 3H), 1.58-1.75 (m, 4H), 2.00 (s, 3H), 3.42 (t, 2H), 3.65 (m, 1H), 4.46 (s, 2H), 5.30 (m, 1H), 7.30 (t, 5H), 7.47 (t, 2H), 7.58 (t, 1H), 7.90 (m, 2H). MS (DCI/NH3) m/e 386 (M+NH ₄ )+.

Example 70C Benzv sw? -f4-acetoxy-5-methyl)-6-hvdroxy-6-phenvπhexyl ether A solution of the compound resulting from Example 70B (0.5 g, 1.4 mmol), CeC • 7 H ₂ 0, and 5 mL of MeOH was stirred at 0 °C as NaBH4 (0.16 g, 4.2 mmol) was added portionwise. The reaction mixture was stirred at 0 °C for 0.25 hours, then 25 mL of 3 N HCI was added (cautiously), followed by the addition of saturated NaCI solution. The solution was extracted with ether (3x). The combined organic layers were washed with saturated NaCI solution, dried (MgSθ4), filtered, and concentrated in vacuo to afford the title compound (0.5 g) as a colorless oil (as a mixture of diastereomers). ¹ H NMR (300 MHz, CDCI ₃ ) δ θ.60 (d, 1.5H), 0.97 (d, 1.5 H), 1.57-1.74 (m, 4H), 1.85-1.98 (m, 1H), 2.02 (s, 1.5H), 2.15 ( s, 1.5H), 3.45 (t, 1H), 3.51 (m, 1H), 4.12 (dd, 0.5H), 4.50 (d, 2H), 4.75 (m, 0.5H), 4.90 (m, 0.5H), 5.43 (m, 0.5H), 7.32 (m, 10H). MS (DCI/NH3) m/e 374 (M+NH ₄ )+.

Example 70D Benzyl \syn -(4-acetoxy-5-methvπ-6-trifluoroacetoxy-6-phenyl]hexyl ether Trifluoroacetic anhydride (0.2 mL, 1.4 mmol) was added dropwise to a 0 °C solution of the compound resulting from Example 70C (0.5 g, 1.4 mmol), pyridine (0.11 mL), and 7 mL CH2CI2. The reaction mixture was stirred at 0 °C for 4.5 hours then quenched with 0.1 N HCI and extracted with CH2CI2 (3x). The combined organic layers were washed with 0.1 N HCI, dried (MgSθ4), filtered, and concentrated in vacuo to afford the title compound (0.59 g) as a colorless oil (as a mixture of diastereomers). ¹ H NMR (300 MHz, CDCI3) δ 0.78 (d, 1.5H), 1.10 (d, 1.5H), 1.50 (m, 1H), 1.64 (m, 2H), 1.78 (m, 1H), 2.02 (d, 3H), 2.32 (m, 1H), 3.39 (t, 1H), 3.50 (m, 2H), 4.98 (d, 2H), 4.67 (m, 0.5H), 5.29 (m, 0.5H), 5.52 (d, 0.5H), 5.78 (d, 0.5H), 7.30 (m, 10H). MS (DCI/NH3) m/e 470 (M+NH ₄ )+.

Example 70E syn -(4-Acetoxy-5-methyl)-6-phenyl-1-hexanol A mixture of the compound resulting from Example 70D (0.59 g, 1.3 mmol), Pd/C (0.16 g, 10%, dry), and 50 mL of EtOAc was hydrogenated in a Parr shaker at room temperature for 39 hours. The mixture was filtered and concentrated in vacuo , and the residue was flash chromatographed on silica gel eluting with 8:2 hexane-EtOAc to afford the title compound (0.18 g) as a colorless oil. ^" Η NMR (300 MHz, CDCI ₃ ) δ 0.89 (d, 3H), 1.45-1.60 (m, 3H), 1.69 (m, 2H), 2.00 (br s, 1H), 2.09 (s, 3H), 2.33 (dd, 1H), 2.77 (dd,1H), 3.64 (t, 2H), 4.92 (m, 1H), 7.08-7.22 (m, 2H), 7.28 (m, 3H). MS (DCI/NH3) m/e 268 (M+NH ₄ )+.

Example 70F 1 -\odo-svn -f4-acetoxy-5-methvπ-6-phenylhexane A solution of the compound resulting from Example 70E (0.33 g, 1.39 mmol) and 9.2 mL anhydrous CH3CN was stirred at room temperature as the following were added sequentially: imidazole (0.24 g, 3.5 mmol), triphenylphosphine (0.40 g, 1.5 mmol), and iodine (0.39 g, 1.5 mmol). The reaction mixture was stirred at room temperature for 1.25 hours, then H2O was added, and the mixture was extracted with CH2CI2. The combined organic layers were washed with saturated sodium thiosulfate solution and saturated NaCI, dried (MgS04), filtered, and concentrated in vacuo to afford a white solid. The solid was triturated with hexane (3x), decanting after each. The hexane layers were combined, concentrated in vacuo , and the residue obtained flash chromatographed on silica gel eluting with 95:5 hexane-EtOAc to afford the title compound (0.38 g) as a colorless oil. ¹ H NMR (300 MHz, CDCI3) δ 1.55 (s, 3H), 1.70 (t, 2H), 1.75-1.86 (m, 2H), 1.99 (m, 1H), 2.09 (s, 3H), 2.34 (dd, 1H), 2.77 (dd, 1 H), 3.20 (t, 2H), 4.90 (m, 1H), 7.10-7.22 (m, 3H), 7.28 (m, 2H). MS (DCI/NH3) m e 378 (M+NH ₄ ) ⁺ .

Example 70G N-Benzyl-N-ft-butyloxycarbonyl -N-f(syt7 -4-acetoxy-5-methyl)-6- phenylhexyllamine A solution of N-benzyl-N-4-butyloxycarbonylamine (0.22 g, 1.05 mmol), prepared by the method described in J. Heterocyclic Chem. 22(5): 1173, (1985), and 0.45 mL of anhydrous DMF was added dropwise to a 0 °C suspension of NaH (0.043 g 1.05 mmol, 60% dispersion, hexane washed) in 1.7 mL of anhyrous DMF. The sodium salt was formed for 0.5 hours at room temperature, then a solution of the compound resulting from Example 70F (0.38 g, 1.05 mmol) in 0.5 mL of anhydrous DMF was added dropwise. The reaction mixture Was stirred for 2 days at room temperature. Ice water was added and the solution was extracted (3x) with ethyl acetate. The combined organic layers were washed with H2O, cold 0.1 N HCI and saturated NaCI solution, dried (MgS04), filtered, and concentrated in vacuo to afford the title compound (0.46 g) as a colorless oil. ¹ H NMR (300 MHz, CDCI3) δ 0.82 (d, 3H), 1.39-1.57 (m, 13H), 1.92 (br s, 1 H), 2.06 (s, 3H), 2.30 (dd, 1 H), 2.72 (dd, 1H), 3.18 (br d, 2H), 4.29-4.49 (m, 2H), 4.85 (s, 1H), 7.10 (d, 2H), 7.19-7.38 (m, 8H). MS (DCI/NH3) m/e 440 (M+H)+, 457 (M+NH ₄ ) ⁺ .

Example 70H

N-Benzyl-N-[(syn -4-acetoxy-5-methyl)-6-phenylhexyl1amine Trifluoroacetic acid (7.7 mL) was added to a 0 °C solution of the compound resulting from Example 70G (0.46 g, 1.07 mmol) and 7.7 mL CH2CI2. The reaction was stirred for 0.5 hours at 0 °C and for 1.5 hours at room temperature. The solvent was evaporated in vacuo . Toluene was added and evaporated in vacuo (2x). Amberlite resin (IRA-400-OH, 0.5 g, washed successively with H2O, EtOH, ether, and dried) and 15 mL of CH2CI2 was added and the suspension was stirred for 18 hours at room temperature. The suspension was filtered and concentrated in vacuo to afford the title compound (0.33 g) as a colorless oil. ¹ H NMR (300 MHz, CDCI3) δ 0.85 (d, 3H), 1.58 (m, 4H), 1.95 (s, 1H), 2.07 (s, 3H), 2.31 (m, 2H), 2.68 (s, 1H), 2.74 (dd, 2H), 3.82 (s, 2H), 4.85 (m, 1H), 7.08-7.22 (m, 3H), 7.28 (m, 3H), 7.37 (m, 4H). MS (DCI/NH3) m/e 340 (M+H)+.

Example 701 5-fN-benzyl-N-fsvn -(4-acetoxy-5-methvπ-6- phenylhexyl}aminocarbonyl]benzene-1.2.4-tricarboxylic acid Using the procedures described in Example 4 but substituting the compound resulting from Example 70H for N-benzyl-N-(4- phenoxybenzyl)amine provided the title compound. ¹ H NMR (300 MHz, DMSO-de) δ 0.63 (d, 3H), 1.16-1.40 (m, 4H), 1.48-1.64 (m, 3H), 1.82 (s, 3H), 2.14-2.38 (m, 1H), 2.89-3.00 (t, 2H), 3.30 ( bs, 3H), 4.02-4.15 (m, 1H), 4.63-4.79 (m, 2H), 7.05 (d, 2H), 7.11-7.18 (m, 2H), 7.19-7.38 (m, 8H), 7.46 (d, 2H). MS (FAB) m/e 576(M+H)+.

Example 71 2-fN-Benzyl-N-C4-phenoxybenzvπaminocarbonyl]pyridine-3-carb oxylic acid A solution of 2,3-ρyridinedicarboxylic anhydride (0.365 g, 2.5 mmol), N- benzyl-N-(4-phenoxybenzyl)amine (0.723 g, 2.5 mmol), diisopropylethylamine (0.32 g, 2.5 mmol), and 20 mL of toluene was refluxed for 1 hour, then evaporated under reduced pressure. The residue was taken up in ethyl acetate, washed with saturated NaCI solution, dried (MgS04), and evaporated. The crude product was purified by flash silica gel chromatography eluting with with 95:4:1 CHCI ₃ -MeOH-HOAc to give 0.28 g of the title compound. 1H NMR (DMSO-de, 300 MHz) δ 4.18 (d, 2H), 4.55 (d, 2H), 6.80-7.50 (m, 14H), 7.60 (m, 1 H), 8.33 (m, 1H), 8.80 (m, 1 H). MS (FAB)+ m/e 439 (M+H)+ and (FAB)- m/e 437 (M-H)+.

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Example 72 4.5-Di|r>l-benzyl-N-(4-phenoxybenzy0aminocarbonyl]benzene -1.2-dicarboxylic acid

Example 72A

1 ,2.4.5-Benzenetetracarboxylic anhydride dichloride A mixture of 15.0 g (68.8 mmol) of 1 ,2,4,5-benzenetetracarboxylic dianhydride in 1200 mL of toluene and 90 mL of dimethylformamide was heated to 65 °C under a nitrogen atmosphere, whereupon the compound dissolved. To this solution was added dropwise 35.7 mL (68.8 mmol) of a 1.93 M solution of phosgene in toluene. After maintaining the temperature at 65 °C for 6 hours, most of the volatiles were removed under reduced pressure, affording an oil. The oil crystallized on standing. Further purification was effected by vacuum sublimation.

Example 72B 4.5-Di[ ^" N-benzyl-N-f4-phenoxybenzvπaminocarbonyllbenzene-1.2- dicarboxylic acid A solution of 300 mg (1.10 mmol) of the compound resulting from Example 72A and 444 mg (2.20 mmol) of triethylamine in 100 mL of tetrahydrofuran was cooled to -30 °C under a nitrogen atmosphere. To this was added by slow dropwise addition 317 mg (1.10 mmol) of N-benzyl-N-(4- phenoxybenzyl)amine in 30 mL of tetrahydrofuran. After 4 hours at -30 °C the solution was allowed to warm to ambient temperature and stirred for 4 hours. All volatiles were removed under reduced pressure, affording an oil. The oil was dissolved in 50 mL of dichloromethane, and the solution was washed with saturated sodium bicarbonate solution. The solution was dried (MgSθ4) and all volatiles were removed under reduced pressure. The oil was dissolved in 50 mL of acetone, and the solution was treated with 50 mL of 1 M HCI. After 24 hours all volatiles were removed under reduced pressure, and the resulting oil was purified by flash column chromatography on silica gel eluting with 180:1:1 followed by 18:1:1 ethyl acetate-formic acid-water to afford 36 mg of the desired

product. 1H NMR (300 MHz, DMSO-d ₆ ) δ 4.3-4.5 (m, 4H), 4.5-4.7 (m, 4H), 6.8- 7.0 (m, 8H), 7.1-7.2 (m, 4H), 7.2-7.5 (m, 18H). MS (FAB)+ m/e 797 (M+H)+.

Example 73 5-fN-Benzyl-N-f4-phenoxybeπzvπaminocarbonyl]-4-fN- benzylaminocarbonyπbenzene-1.2-dicarboxylic acid A mixture of 500 mg (2.29 mmol) of 1 ,2,4,5-benzenetetracarboxylic dianhydride in 20 mL of acetone under an atmosphere of nitrogen was cooled to -30 °C, whereupon a solution of 662 mg (2.29 mmol) of N-benzyl-N-(4- phenoxybenzyl)amine and 296 mg (2.29 mmol) of diisopropylethylamine in 10 mL of acetone was added dropwise. The solution was allowed to warm to ambient temperature. After 24 hours all volatiles were removed under reduced pressure. The resulting oil was dissolved in 50 mL of methylene dichloride, and the solution was washed with dilute hydrochloric acid, dried (MgSθ4) and then treated with 1 mL of thionyl chloride. After 6 hours all volatiles were removed under reduced pressure, affording an oil. The oil was dissolved in 20 mL of methylene dichloride and a solution of 245 mg (2.29 mmol) of benzylamine and 296 mg (2.29 mmol) of diisopropylethylamine in 10 mL of methylene dichloride was added dropwise. After 6 hours the mixture was washed with dilute hydrochloric acid, dried (MgSθ4), and concentrated under reduced pressure to afford an oil. The oil was dissolved in 50 mL of acetone, and the solution was treated with 50 mL of 1 M HCI. After 24 hours all volatiles were removed under reduced pressure. Purification by flash column chromatography on silica gel eluting with 18:1:1 ethyl acetate-formic acid-water afforded 42 mg of the desired product. ¹ H NMR (300 MHz, CD ₃ OD) δ 4.1-4.2 (m, 2H), 4.4-4.5 (m, 2H), 6.7-6.8 (m, 1H), 6.8-6.9 (m, 3H), 6.9-7.1 (m, 3H),7.2-7.4 (m, 14H). MS (FAB)+ m/e 615 (M+H)+.

Example 74a 3-fN-Benzyl-N-f4-phenoxybenzyl)aminocarbonvπbenzophenone-3' .4'.4- tricarboxylic acid and Example 74b

3'.4-DifN-Benzyl-N-(4-phenoxybenzvπaminocarbonyπbenzoph enone-3.4'- dicarboxylic acid To a solution of 1.67 g (5.19 mmol) of 3,3',4,4'- benzophenonetetracarboxylic dianhydride in 100 mL of acetone cooled to 0 °C was added a solution of 1.50 g (5.19 mmol) of N-benzyl-N-(4- phenόxybenzyl)amine and 0.57 g (5.71 mmol) of triethylamine dropwise. The solution was allowed to slowly warm to ambient temperature. After 6 hours all volatiles were removed under reduced pressure, the resulting oil was dissolved in ethyl acetate, and the solution was washed with dilute hydrochloric acid. The solution was then stirred vigorously with 10% hydrochloric acid for 24 hours. The organic phase was separated, dried (MgS04), and concentrated under reduced pressure. The resulting oil was purified by flash column chromatography on silica gel eluting with 18:1:1 ethyl acetate-formic acid-water to give 260 mg of 74a and 1.41 g of 74b. Example 74a: ¹ H NMR (300 MHz, DMSO-de) δ 3.8-3.9 (m, 2H), 4.2-4.3 (m, 2H), 6.8-7.5 (m, 14H), 7.6-8.1 (m, 6H). MS (FAB)+ m/e 630 (M+H)+. Example 74b: ^H NMR (300 MHz, CDCI ₃ ) δ 3.8- 3.9 (m, 2H), 4.1-4.3 (m, 4H), 4.8-5.2 (m, 2H), 6.8-7.2 (m, 12H), 7.2-7.4 (m, 12H), 7.5-8.3 (m, 10H). MS (FAB)+ m/e 901 (M+H)+.

Example 75 7-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonvπnaphthalene-2.3 .6- tricarboxylic acid and Example 76

3.7-DirN-benzyl-N-f4-phenoxybenzvπamino-carbonvnnaphthal ene-2.6- dicarboxylic acid 2,3,6,7-Naphthalenetetracarboxylic acid dianhydride was prepared using the procedure of Boykin, D.W., Nowak-Wydra, B., and Baumstark, A.L., J. Het. Chem. 28: 609 (1991). The title compounds were prepared by the procedures described in Examples 74a and 74b. Example 75: ¹ H NMR (300 MHz, DMSO-de) δ 4.3-4.4 (m, 2H), 4.4-5.0 (bs, 2H), 6.8-7.1 (m, 4H), 7.1-7.5 (m, 12H), 8.24 (d, 1H), 8.69 (d, 1H). MS (FAB)+ m/e 576 (M+H)+. Example 76: 1H NMR (300 MHz, DMSO-d ₆ ) δ 4.2-4.3 (m, 4H), 4.4-5.0 (bs, 4H), 6.8-7.1 (m, 9H), 7.1-7.5 (m, 23H), 8.24 (d, 1H), 8.69 (d, 1H). MS (FAB)+ m/e 847 (M+H)+.

Example 77 5-| ^" N-Benzyl-N-^fluoren-2-ylmethyl)aminocarbonyl1benzene-1 .2.4-tricarboxylic acid N-Benzyl-N-(fluoren-2-ylmethyl)amine was prepared by the procedures described for the preparation of N-benzyl-N-(4-phenoxybenzyl)amine. The title compound was prepared in the same manner as Example 74a. ¹ H NMR (300 MHz, DMSO-de) δ 3.84 (s, 2H), 4.24 (d, 2H), 4.3-5.0 (bs, 2H), 7.1-7.4 (m, 10H), 7.5-7.7 (m, 2H)7.8-8.1 (m, 2H). MS (FAB)+ m/e 522 (M+H)+.

Example 78 5-[N-Benzyl-N-^4-phenoxybenzyl)aminothiocarbonyl]-1.3- dimethoxycarbonylbenzene A slurry of 5-[N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-1 ,3- dimethoxycarbonylbenzene (1.03 g, 2.0 mmol) and Lawesson's reagent (404 mg, 1.0 mmol) in THF (7 mL) was stirred at reflux for 18 hours then cooled to room temperature. Silica gel (50 g) was added to the reaction mixture which was then concentrated to dryness. The residue was poured onto a prepacked silica gel column and eluted with 15% ethyl acetate-hexane to provide 927 mg, (88%) of the title compound as a yellow solid. ¹ H NMR (CDCI ₃ , 300 MHz) δ

3.58 (s, 3H), 3.59 (s, 3H), 4.6 (d, 2H), 5.4 (d, 2H), 7.0 (m, 8H), 7.4 (m, 6H), 8.2 (m 2H), 8.6 (m, 1 H). MS (DCl) m e 526 (M+H)+.

Example 79 3-Methoxycarbonyl-5-fN-benzyl-N- ⁽ 4-phenoxybenzvhaminothio- thiocarbonyl]benzoic acid A solution of 5-[N-benzyl-N-(4-phenoxybenzyl)aminothiocarbonyl]-1 ,3- dimethoxycarbonylbenzene (716 mg, 1.4 mmol) in THF (21 mL) and water (7 mL) at 0 °C was treated with 1 N LiOH (1.4 mL). After 6 hours at 0 °C and 18 hours at room temperature, the reaction mixture was concentrated to remove THF, acidified to pH 0 with 1 N HCI and extracted with ethyl acetate (2 x 50 mL). The combined organic extracts were dried (MgSθ4), filtered and concentrated to provide a yellow solid. The solid was chromatographed eluting with a gradient eluant of 1% MeOH in CHCI3 going to 98:1:1 CHCI ₃ -MeOH-acetic acid to provide 353 mg (50%) of the title compound as a yellow solid. ¹ H NMR (CDCI ₃₎ 300 MHz) δ 3.55 (s, 3H), 4.6 (d, 2H), 5.4 (d, 2H), 7.0 (m, 8H), 7.4 (m, 6H), 8.2 (m, 2H), 8.6 (m, 1H), 12.5 (br s, 1H). MS (DCl) m/e 512 (M+H)+.

Example 80 5-[N-Benzyl-N-(4-phenoxybenzvπaminothiocarbonvπbenzene-1.3 - dicarboxylic acid A solution of the compound resulting from Example 78 (1.18 g, 2.2 mmol) in THF (45 mL) and water (33 mL) was treated with 1 LiOH (13.4 mL). After 18 hours the THF was removed, and the aqueous solution was acidified to pH 0 with 1 ϋ HCI and extracted with ethyl acetate (2 x 50 mL). The combined organic extracts were dried (MgS04), filtered and concentrated to provide a yellow solid. The solid was chromatographed eluting with 98:1 :1 CHCI3- MeOH-acetic acid to provide 97 mg (87%) of the title compound as a yellow solid. 1H NMR (DMSO-de, 300 MHz) δ 4.7 (d, 2H), 5.5 (d, 2H), 7.0 (m, 8H), 7.4 (m, 6H), 8.2 (m, 2H), 8.6 (m, 1 H), 12.5 (br s, 2H). MS (FAB) m/e 498 (M+H)+.

Example 81 N-Benzyl-N-(3.5-dimethoxycarbonylbenzyπ-N-f4-phenoxybenzvπ amina

A slurry of the compound resulting from Example 78 (2 g, 3.8 mmol) and Raney nickel (4.5 g, 77 mmol) in methanol (50 mL) was stirred for 18 hours at room temperature then filtered through celite. The filtrate was concentrated in vacuo and chromatographed eluting with 10% ethyl acetate in hexane to provide 483 mg (26%) of the title compound as a colorless oil. H NMR

(CDCI3, 300 MHz) δ 3.54 (s, 2H), 3.56 (s, 2H), 3:65 (s, 2H), 3.95 (m, 6H), 6.9-7.1 (m, 6H), 7.2-7.45 (m, 8H), 8.25 (s, 2H), 8.55 (s, 1H). MS (DCl) m e 496 (M+H)+.

Example 82 5-[N-Benzyl-N-f4-phenoxybenzvπaminomethyπbenzene-1.3-dicar boxylic acid disodium salt A solution of the compound resulting from Example 78 (1.1 g, 2.16 mmol) and NaOH (173 mg, 4.32 mmol) in water (7 mL) at room temperature was treated with Raney nickel (2.5 g, 43.2 mmol) and stirred for 18 hours. The solution was filtered through celite and then lyophilized. The resulting white solid was redissolved in water and gradient eluted through a C-iβ reversed phase column eluting with water with an increasing CH3CN concentration to provide 871 mg (79%) of the title compound as a white solid. ¹ H NMR (D2O,

300 MHz) δ 3.6 (d, 2H), 4.8 (d, 2H), 5.4 (d, 2H), 6.8-7.1 (m, 7H), 7.2-7.45 (m, 7 H), 7.8 (dd, 1H), 7.9 (dd, 1H), 8.2 (dd, 1H). MS (FAB) m/e 512 [M+H, (diacid)]+.

Example 83 2-rN-Benzyl-N-f4-phenoxybenzyπaminocarbonyl ^" l-1.4- dimethoxycarbonylbenzene

Example 83A 2.5-Dimethoxycarbonylbenzoic acid A slurry of dimethyl iodoterephthalate and sodium acetate in 1:1 DMF-

H2O (56 mL) was treated with palladium acetate (32 mg) and stirred at 50 °C for 18 hours under an atmosphere of carbon monoxide. The solution was acidified to pH 0 and extracted with ethyl acetate. The combined organic extracts were dried (MgS04), filtered and concentrated to provide an oil. Chromatography of the oil eluting with 98:1:1 CHCl3-MeOH-acetic acid provided 200 mg (6%) of the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 3.95 (s, 3H), 4.0 (s, 3H), 7.75 (d, 1H), 8.3 (dd, 1H), 8.6 (d, 1H), 11.2 (br s, 1H). MS (DCl) m/e 239 (M+H)+.

Example 83B

2-fN-Benzyl-N- 4-phenoxybenzvπaminocarbonyl]-1.4- dimethoxycarbonylbenzene A solution of the compound resulting from Example 83A (42 mg, 0.18 mmol) in toluene (1 mL) was treated with oxalyl chloride (25 mg, 0.19 mmol) and DMF (2 drops). After 18 hours, the solution was concentrated to dryness, and the 2,5-dimethoxycarbonylbenzoyl chloride thus obtained was dissolved in CH2CI2 and added dropwise to a slurry of N-benzyl-N-(4- phenoxybenzyl)amine hydrochloride (117 mg, 0.36 mmol) and NaHCθ3 (151 mg, 1.8 mmol) in water (3 mL). After 18 hours, the CH2CI2 layer was dried (MgSθ4), filtered and concentrated. The residue was chromatographed on silica gel eluting with 15% ethyl acetate in hexane to provide 89 mg (98%) of the title compound as a white solid. ^" Η NMR (CDCI3, 300 MHz) δ 3.8 (d, 2H),

3.79 (d, ^' 2H), 3.85 (s, 3H), 3.86 (s, 3H), 4.2 (d, 2H), 6.9-7.15 (m, 7H), 7.25-7.45 (m, 5H), 8.0-8.1 (m, 3H). MS (DCl) 510 (M+H)+.

Example 84 2-[N-Benzyl-N-f4-phenoxybenzyπaminocarbonyl1benzene-1.4-dic arboxylic acid A solution of the compound resulting from Example 83 (25 mg, 0.049 mmol) in THF (750 μL) and water (250 μL) was treated with 1 N LiOH (290 μL). After 18 hours, the solution was acidified to pH 0 with 1 N HCI, concentrated to remove THF and extracted with ethyl acetate (2 x 2 mL). The combined organic extracts were dried (MgS04), filtered and concentrated, and the residue was chromatographed on silica gel eluting with 98:1 :1 CHC -MeOH-acetic acid to provide 23 mg (98%) of the title compound as a white solid. ¹ H NMR (DMSO- de, 300 MHz) δ 4.09 (s, 2H), 4.11 (s, 2H), 6.9-7.25 (m, 14H), 7.8 (d, 1H), 8.0 (m, 2H), 13.5 (br s, 2H). MS (FAB) m/e 482 (M+H)+.

Example 85

2-[N-f4-phenoxybenzvncarbonylamino]-1.4-dimethoxycarbonyl benzene 4-Phenoxyphenylacetic acid (1 g, 4.4 mmol) in toluene (15 mL) was treated with oxalyl chloride (614 mg, 4.84 mmol) and DMF (2 drops). After 18 hours, the reaction was concentrated to dryness, and the residue was dissolved in CH2CI2 (20 mL) and added to a suspension of dimethyl aminoterephthalate (920 mg, 4.4 mmol) and NaHCθ3 (4.7 mg, 44 mmol) in water (30 mL). After 18 hours, the methylene chloride was dried (MgS04), filtered and concentrated to provide a yellow solid which was chromatographed on silica gel eluting with 15% ethyl acetate in hexane to provide 1.4 g (76%) of the title compound as a white solid. 1H NMR δ 3.8 (s, 2H), 3.95 (s, 3H), 3.96 (s, 3H), 7.05 (m, 5H), 7.35 (m, 4H), 7.75 (dd, 1 H), 8.1 (d, 1 H), 9.35 (d, 1 H), 11.0 (br s, 1 H). MS (DCl) m/e 420 (M+H)+.

Example 86 2-rN-f4-Phenoxybenzvπcarbonylamino1benzene-1.4-dicarboxylic acid The compound resulting from Example 85 (620 mg, 1.5 mmol) in methanol (7 mL) and water (7 mL) was treated with 87 % KOH (954 mg, 14.8 mmol). After 18 hours, the solution was acidified to pH 0 with 1 N HCI and concentrated to remove the methanol. The aqueous layer was extracted with ethyl acetate. The combined organic extracts were dried (MgS04), filtered and concentrated. The residue was chromatographed on silica gel eluting with 98:1:1 CHC -MeOH-acetic acid to provide 563 mg (97%) of the title compound as a white solid. ¹ H NMR (CDCI ₃ , 300 MHz) δ 3.8 (s, 2H), 7.0 (m, 3H), 7.15 (m, 2H), ^' 7.4 (m, 4H), 7.65 (dd, 1 H), 8.1 (d, 1 H), 9.1 (d, 1H), 11.1 (br s, 1H), 13.3 (br s, 2H). MS (DCl) m/e 409 (M+NH ₄ )+.

Example 87 4-[N-Benzyl-N-( ^' 4-phenoxybenzy0carbonylamino ^" l-1.2- dimethoxycarbonylbenzene

Example 87A Dimethyl 4-aminophthalate A slurry of dimethyl 4-nitrophthalate (5.37 g, 22.5 mmol) and ammonium formate 1.42 g, 225 mmol) in methanol (75 mL) was treated with 10% palladium on carbon (500 mg), and the resulting mixture was stirred at reflux for 3 hours. The reaction mixture was cooled, filtered through celite and concentrated to provide the title compound as a white solid used directly in the next step. 1 HNMR (CDCI3, 300 MHz) δ 3.8 (s, 3H), 3.9 (s, 3H), 4.2 (br s, 2H), 6.65 (dd, 1 H), 6.7 (d, 1H), 7.7 (d, 1H). MS (DCl) m/e 210 (M+H)+.

Example 87B 4-[N-f4-Phenoxybenzvπcarbonylamino1-1.2-dimethoxycarbonylbe nzene 4-Phenoxyphenylacetic acid (1g, 4.4 mmol) in toluene (15 mL) was treated with oxalyl chloride (614 mg, 4.8 mmol) and DMF (2 drops). After 18 hours, the reaction was concentrated to dryness, and the residue was dissolved in CH2CI2 (20 mL) and added dropwise to a slurry of NaHCU3 (4.64 g, 43.8 mmol) and the compound resulting from Example 87A (920 mg, 4.4 mmol) in water (30 mL). After 18 hours, the methylene chloride layer was dried (MgSθ4), filtered and concentrated, and the residue was chromatographed on silica gel eluting with 15% ethyl acetate in hexane to provide 1.2 g (65 %) of the title compound as a white solid. H NMR (CDCI3, 300 MHz) δ 3.9 (s, 3H), 4.0 (s, 3H), 4.5 (brs, 1H), 4.6 (d, 2H), 6.8-7.7 (m, 11H), 7.7 (d, 1H). MS (DCl) m/e 420 (M+H)+.

Example 87C

4-[N-Benzyl-N-( ^, 4-phenoxybenzvπcarbonylaminol-1.2- dimethoxycarbonylbenzene A slurry of benzyl bromide (558 mg, 3.3 mmol) and 60 % sodium hydride (65 mg, 1.6 mmol) in DMF (10 mL) was treated with the compound resulting from Example 87B (639 mg, 1.6 mmol) and stirred for 18 hours at room temperature. Water (20 mL) was added, and the suspension was extracted with ethyl acetate (3 x 20 mL). The ethyl acetate was dried (MgSθ4), filtered and concentrated to provide a residue which was chromatographed on silica gel eluting with 15 % ethyl acetate in hexane to provide 722 mg (87 %) the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 3.9 (s, 3H), 4.0 (s, 3H), 4.6 (d, 2H), 5.2 (d, 2H), 6.8-7.7 (m, 16H), 7.7 (d, 1H). MS (DCl) m/e 510 (M+H)+.

Example 88

3-Methansulfonylamino-5-rN-benzyl-N-(4- phenoxybenzvπaminocarbonyllbenzoic acid

Example 88A

3-Nitro-5-fN-benzyl-N-f4-phenoxybenzvnaminocarbonvπbenzo ic acid methyl ester To a stirred suspension of 3-nitroisophthalic acid monomethyl ester (2.25 g, 10 mmol, 1.0 eq.) in 50 mL of CH2CI2 was added 1.05 mL (12 mmol, 1.2 eq.) of oxalyl chloride followed by 3 drops of DMF. The solution was stirred at room temperature for 2 hours and concentrated to dryness. The resulting white solid was redissolved in 25 mL of CH2CI2 and 3.42 g (10.5 mmol, 1.05 eq.) of N- benzyl-N-(4-phenoxybenzyl)amlne ^• HCI was added, and the suspension was cooled to 0 °C. Triethylamine (4.2 mL, 30 mmol, 3 eq.) was added dropwise, and the resulting solution was stirred overnight while the ice bath melted. The mixture was poured into a separatory funnel and extracted with water, 3 N[ aqueous HCI, dried, filtered and concentrated to give the title compound (5.04 g, 101%) as a thick oil. ^" >H NMR (300MHz., CDCI3) δ 7.50 (t, 1H), 7.20 - 7.44 (m, 8H), 7.13 (m, 3H), 7.04 (m, 5H), 4.69 (bd, 2H), 4.43 (bd, 2H), 3.89 (s, 3H). MS (DCl, NH ₃ ) m/e 514 (8%); 290 (100%).

Example 88B 3-Amino-5-rN-benzyl-N-^4-phenoxybenzvπaminocarbonyl1benzoic acid methyl ester To a stirred solution of 2.48 g (5 mmol, 1.0 eq.) of the compound resulting from Example 88A in 40 mL of methanol was added 500 mg of 10% Pd/C followed by 1.58 g (25 mmol, 5 eq.) of NH4+HC02 ^" , and the mixture was heated to reflux for 1 hour and then cooled to room temperature. The suspension was diluted with ethyl ether and filtered through a plug of Siθ2 (prewetted with ether) and the pad washed well with ether. The filtrate was concentrated to give 1.94 g (83%) of the title compound as a white foam. ¹ H NMR (300 MHz., CDCI3) δ 8.86 (t, 1 H), 8.47 (m, 1 H), 8.43 (t, 1H), 7.37 (m, 7H), 7.12 (m, 2H), 7.01 (m, 5H), 4.73

(bd, 2H), 4.41 (bd, 2H), 3.98 (s, 3H). MS (DCl, NH ₃ ) m/e 484 (25%); 467 (100%).

Example 88C 3-Methansulfonylamino-5-fN-benzyl-N-(4- phenoxybenzyπaminocarbonvπbenzoic acid methyl ester To a stirred solution of 233 mg (0.5 mmol, 1.0 eq) of the compound resulting from Example 88B in 5 mL of CH2CI2 at 0 °C was added 0.12 mL (1.0 mmol, 2.0 eq.) of 2,6-lutidine follwed by the addition of 42 μL (0.55 mmol, 1.1 eq.) of methanesulfonyl chloride. After stirring for 48 hours, the mixture was diluted with CH2CI2 and extracted with 3 N aqueous HCI, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (25 g) eluting with 40% ethyl acetate in hexanes to give 162 mg (60%) of the title compound as a white foam. H NMR (300MHz., CDCI3) δ 7.93 (t, 1H), 7.88 (bs, 1 H), 7.57 (bs, 1 H), 7.22 - 7.42 (m, 7H), 7.13 (m, 2H), 7.04 (m, 5H), 4.71 (bd, 2H), 4.39 (bd, 2H), 3.91 (bs, 3H), 3.97 and 3.92 (2s, 3H). MS (DCl, NH ₃ ) m/e 562 (100%); 545 (20%); 453 (25%); 290 (38%).

Example 88D 3-Methansulfonylamino-5-fN-benzyl-N-(4- phenoxybenzvhaminocarbonyl]benzoic acid The title compound was prepared as a white lyophilate from the compound resulting from Example 88C and using the procedures described in Example 116B. ^" -H NMR (300MHz., DMSO-d ₆ ): δ 11.1 (s, 1H), 7.85 (s, 1H), 7.64 (s, 1 H), 7.47 (t, 1 H), 7.24 - 7.43 (m, 7H), 7.14 (m, 3H), 6.99 (m, 4H), 4.62 (bd, 2H), 4.43 (bs, 2H), 2.96 (bs, 3H). MS (DCl, NH ₃ ) m/e 548 (75%); 531 (50%); 381 (20%); 366 (100%); 349 (20%); 200 (16%); 183 (20%). Anal calcd for C29H26N2O6S ^• 0.49 H ₂ 0: C, 65.65; H, 4.94; N, 5.28. Found: C, 64.58; H, 4.99; N, 5.40.

Example 89 4-fN-Benzyl-N-f4-phenoxybenzylcarbonvnamino1benzene-1.2-dica rboxylic acid A solution of the compound resulting from Example 87 (310 mg, 0.61 mmol) in methanol (3 mL) and water (3 mL) was treated with 87 % KOH (34 mg, 6.1 mmol) and stirred for 18 hours at room temperature. The solution was acidified to pH 0 with 1 N HCI, concentrated to remove methanol and extracted with ethyl acetate (2 x 10 mL). The ethyl acetate was dried (MgS04), filtered and concentrated to provide a residue which was chromatographed eluting with 98:1 :1 CHCl3-MeOH-acetic acid to provide 287 mg (98 %) of the title compound as a white solid. ¹ H NMR (DMSO-de, 300 MHz) δ 4.5 (d, 2H), 5.1 (d, 2H), 6.8-7.5 (m, 16H), 7.6 (d, 1H), 13.4 (br s, 2H). MS (FAB) m/e 482 (M+H)+.

Example 90 2-[ ^" N-Benzyl-N-f4-phenoxybenzyπaminocarbonylamino -1.4- dimethoxycarbonylbenzene A solution of 2,5-dimethoxycarbonylbenzoic acid (100 mg, 0.42 mmol) in toluene (2 mL) was treated with diphenylphosphoryl azide (127 mg, 0.46 mmol) and triethylamine (85 mg, 0.84 mmol) then warmed to 80 °C for 18 hours. N- Benzyl-N-(4-phenoxybenzyl)amine hydrochloride (137 mg, 0.42 mmol) was added, and the brown mixture was stirred an additional 18 hours. Volatiles were removed, and the residue was dissolved in ethyl acetate (5 mL) and washed with 1 N HCI (5 mL), half saturated NaHCθ3 (5 mL) and brine (5 mL). The ethyl acetate layer was dried (MgS04), filtered, concentrated and chromatographed on silica gel eluting with 15% ethyl acetate in hexane to provide 190 mg (86%) of the title compound as a colorless oil. ¹ H NMR (CDCI _3| 300 MHz) δ 3.9 (s, 3H), 3.91 (s, 3H), 4.4 (d, 2H), 4.6 (d, 2H), 5.8 (br s, 1H), 6.8-7.7 (m, 16H), 7.9 (d, 1H). MS (DCl) m e 525 (M+H)+.

Example 91 2-fN-Benzyl-N-(4-phenoxybenzvhaminocarbonylamino1benzene-1.4 - dicarboxylic acid The compound resulting from Example 90 (17.4 mg, 0.03 mmol) in water (500 μL) and THF (166 μL) at room temperature was treated with 1 JNJ LiOH (330 μL) and stirred for 18 hours. The solution was acidified to pH 0 with 1 HCI and concentrated to remove THF. The water layer was extracted with ethyl acetate (1 mL). The ethyl acetate was dried (MgS04), filtered and concentrated to provide a residue which was chromatographed on silica gel eluting with 98:1:1 CHCl3-MeOH-acetic acid to provide 10.1 mg (62%) of the title compound as a white solid. 1H NMR (DMSO-d ₆ , 300 MHz) δ4.5 (d, 2H), 4.6 (d, 2H), 5.(br s, 1H), 6.5-7.6 (m, 16H), 7.9 (d, 1H), 12.5 (br s, 2H). MS (FAB) m/e 497 (M+H)+.

Example 92 5-{N-Benzyl-N-[2.4-di ⁽ 4-chlorophenoxy benzyl]aminocarbonyl}benzene-1.2.4- tricarboxylic acid

Example 92A 2.4-Di(4-chlorophenoxy)benzonitrile A mixture of 2,4-difluorobenzonitrile (2.68 g, 20 mmol), 4-chlorophenol

(7.72 g, 60 mmol), and anhydrous potassium carbonate (8.29 g, 60 mmol) in anhydrous DMF (60 mL) was refluxed 15 hours. The reaction mixture was then poured to a separation funnel containing water (50 mL) and ether (300 mL). The organic layer was separated and was further washed with aqueous sodium hydroxide (2.0 M, 20 mL), water (40 mL), half saturated ammonium chloride (50 mL), and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then redissolved in a minimum amount of hot ethyl acetate and then gradually cooled to 0 °C. The crystals were collected by suction filtration, washed with cold ether, and air dried to give the title compound (5.87 g, 80%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.57 (d, 1 H), 7.36 (m, 4 H), 7.04 (dt, 2 H), 6.97 (dt, 2 H), 6.62 (dd, 1 H), 6.47 (d, 1 H).

Example 92B 2.4-Di(4-chlorophenoxy)benzylamine hydrochloride To a 0 °C solution of the compound resulting from Example 92A (5.83 g, 16.4 mmol) in THF (40 mL) was added slowly a solution of lithium aluminum hydride (1.0 M in THF, 16.4 mmol). The reaction mixture was reluxed for 5 hours and then cooled to 0 °C. To it was slowly added water (0.65 mL), 15% aqueous NaOH (0.65 mL), and water (2 mL), and the mixture was stirred at 25 °C for 30 minutes, and then filtered through Celite. The filtrate was concentrated, redissolved in ether (50 mL), and cooled to 0 °C. Concentrated hydrochloric acid (2 mL) was then added dropwise to the mixture with good stirring. The white precipitate was collected by suction filtration and dried under high vacuum to give the title compound as a HCI salt (5.75 g, 88%). ¹ H NMR (300 MHz, DMSO-de) δ 8.28 (br. s., 3H), 7.59 (d, 1H), 7.50 (dt, 2H), 7.45 (dt, 2H), 7.15 (dt, 2H), 7.04 (dt, 2H), 6.83 (dd, 1H), 6.48 (d, 1H), 4.04 (q, 2H).

Example 92C N-Benzyl-N-f2.4-di(4-chlorophenoxy)benzvπamine The compound resulting from Example 92B (799 mg, 2.0 mmol) was mixed with benzaldehyde (203 μL, 2.0 mmol) in ethanol (5 mL). After 1 hour, acetic acid (200 mg), sodium acetate (300 mg), and sodium cyanoborohydride (1.0 M in THF, 2.0 mL) was added to the mixture. After 15 hours, concentrated hydrochloric acid (0.5 mL) was slowly added to the reaction mixture, followed by addition of 20% aqueous NaOH (8 mL) over 10 minutes. The mixture was then diluted with ether (80 mL), washed with water and brine, dried over anhydrous potassium carbonate, filtered, and concentrated in vacuo. The residue was then purified with column chromatography eluting with 50% ether in hexane to give the title compound (507 mg, 56%). ¹ H NMR (300 MHz, DMSO-de) δ 7.42-7.22 (m, 10H), 6.92 (d, 2H), 6.87 (d, 2H), 6.74 (d, 1H), 6.53 (s, 1H), 3.79 (s, 4H).

Example 92D 5-{N-Benzyl-N-f2.4-di(4-chlorDhenoxy^benzyl1aminocarbonyl}be nzene-1.2.4- tricarboxylic acid Using the procedure described in Example 4 and the compound resulting from Example 92C the title compound was obtained in 42% yield. ^" Η NMR (500 MHz, CDCI ₃ ) δ 8.30, 8.22,8.00, 7.94 (4 singlets, 2H), 7.73-6.63 (m, 15H), 6.52, 6.48 (2 triplets, 1H), 4.31, 4.09 (2 br. singlets, 4H). MS (FAB -) m/e 684 (M-H).

Example 93 f± 5-rN-flndan-2-vπ-N-f4-phenoxybenzvπaminocarbonvnbenzene-1. 2.4- tricarboxylic acid

Example 93A (±VN-flndan-2-vfl-N-(4-phenoxy benzylamine

The procedure described in Example 92C was used to combine (±)-2- aminoindane (266 mg, 2 mmol) and 4-phenoxybenzaldehyde (396 mg, 2 mmol) to give the title compound (628 mg, 100%) without purification. ¹ H NMR (300 MHz, CDCI3) δ 7.44-6.97 (m, 13H), 4.33 (t, 1H), 3.91 (d, 1 H), 3.87 (d, 1 H), 3.05 (m, 1 H), 2.82 (m, 1 H), 2.44 (m, 1 H), 1.94 (m, 1 H).

Example 93B fe)-5-rN-πndan-2-vn-N-f4-phenoxy)benzylaminocarbonynbenzene -1.2.4- tricarboxylic acid The procedure decribed in Example 4 was used to react the compound resulting from Example 93A (2 mmol) and 1 ,2,4,5-benzenetetracarboxylic dianhydride (2 mmol) to give the title compound (757 mg, 69%). 1H NMR (300 MHz, DMSO-d ₆ ) δ 8.40 and 8.29 (2 singlets, 1H), 7.98 and 7.83 (2 singlets, 1 H), 7.44-6.74 (m, 13H), 5.11 (t, 1H), 4.80-4.60 and 4.30-4.10 (2 m's, 2H), 2.80 and 2.70 (2 m's, 2H), 2.18 and 1.95 (2 m's, 2H). MS (FAB-) m/e 550 (M-H).

Example 94 (± 5-rN-πndan-1-vπ-N-f4-phenoxy)benzylaminocarborivnbenzene-1 .2.4- tricarboxylic acid The title compound was prepared by the procedures described in Example 93 starting with 1-aminoindane instead of the (±)-2-aminoindane compound. ¹ H NMR (500 MHz, DMS0-d ₆ ) δ 8.60-8.00 (4 singlets, 2 H), 7.43- 6.90 (m, 13 H), 4.80-4.60 (2 br. singlets, 2 H), 4.35 (m, 1 H), 3.35-2.80 (m, 4 H). MS (FAB-) m/e 550 (M-H).

Example 95

5-[N-ffrθ-α-Carbomethoxybenzvfl-N-(4- phenoxybenzyhaminocarbonyl]benzene-1.2.4-tricarboxylic acid

Example 95A N-((±)-α-CarbomethoxybenzvD-N-f4-phenoxybenzvπamine

The procedure described in Example 92C was used to combine (±)- phenylglycine (4.03 g, 20 mmol) and 4-phenoxybenzaldehyde (3.88 g, 19.6 mmol) to give the title compound (5.51 g, 79%), after column purification. ¹ H NMR (300 MHz, CDCI ₃ ): δ 7.40-7.28 (m, 9 H), 7.10 (m, 1 H), 7.02-6.95 (m, 4 H), 4.41 (s, 1 H), 3.70 (s, 5 H).

Example 95B 5-[N-^+ x-Carbomethoxybenzvπ-N-f4- phenoxybenzvhaminocarbonyl)benzene-1.2.4-tricarboxylic acid The procedures decribed in Example 4 were used to react the compound resulting from Example 95A (347 mg, 1 mmol) and 1,2,4,5- benzenetetracarboxylic dianhydride (262 mg, 1.2 mmol) to give the title compound (491 mg, 84%). ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 8.82 and 8.74 and 8.18 and 8.16 (4 singlets, 2H), 7.50 -6.60 (m, 14H), 5.30 (br. s., 1H), 4.38-4.00 (m, 2H), 3.73 and 3.67 (2 singlets, 3H). MS (FAB -) m/e 582 (M-H).

Example 96 4-fN-(teVα-Carbomethoxybenzy0-N-(4- phenoxybenzvπaminocarbonyllbenzene-1 ,2-dicarboxylic acid To a solution of trimellitic anhydride chloride (505 mg, 2.4 mmol) at 0 °C was added a solution of the compound resulting from Example 95A (695 mg, 2.0 mmol) over 1.5 hours. After another 15 hours, the reaction was quenched with aqueous 10% sodium carbonate (10 mL). After 30 minutes, the mixture was acidified with aqueous 3 M hydrochloric acid to pH ~2. The resulting mixture was extracted with ethyl acetate (2 x 60 mL), and the combined organic extracts were dried over magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 94:5:1 ethyl acetate-methanol-formic acid to give the title compound (917 mg, 85%). 1H NMR (500 MHz, DMSO-d ₆ ) δ 8.00-6.90 (m, 17H), 5.70 (br. s, 1H), 4.60-4.35 (m, 2H), 3.70 (br. s, 3H). MS (FAB -) m/e 538 (M-H).

Example 97 4-| ^" N-f( ^, ± (x-Carboxybenzyπ-N-f4-phenoxybenzyπaminocarbonyl]benzene-1 ,P- dicarboxylic acid A mixture of the compound resulting from Example 96 (323 mg, 0.60 mmol) and aqueous NaOH (2 JNJ, 1 mL) in THF (3 mL) was stirred at room temperature for 72 hours. The mixture was then acidified with aqueous 3 N HCI (1 mL) and extracted (4 x 20 mL) with 1:4 methanol-dichloromethane mixed solvent. The combined extracts were dried over sodium sulfate, filtered and concentrated. The residue was purified by column chromatography eluting with 95:5:2 ethyl acetate-methanol-formic acid to give the title compound (297 mg, 95 %). 1H NMR (500 MHz, DMSO-d ₆ ) δ 7.80-6.93 (m, 17 H), 5.65 (br. s, 1 H), 4.60-4.35 (m, 2 H). MS (FAB -) m/e 524 (M-H).

Example 98 4-f3-Phenyl-2-^4-phenoxybenzvhpropionyl]benzene-1.2-dicarbox ylic acid

Example 98A Dimethyl 4-vinylphthalate

To a solution of dimethyl 4-hydroxyphthalate (2.10 g, 10.0 mmol) and pyridine (1.6 mL, 20 mmol) in dichloromethane (20 mL) at -78 °C was added slowly trifluoromethanesulfonic anhydride (2.02 mL, 12 mmol). After the reaction was allowed to warm to room temperature overnight, it was diluted with ether (80 mL), washed with water, saturated copper(ll) sulfate and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 20% ethyl acetate in hexane to give the desired triflate (3.37 g, 98%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.83 (d, 1H), 7.66 (d, 1H), 7.48 (dd, 1H), 3.66 (s, 3H), 3.64 (s, 3H). A mixture of the triflate (2.39 g, 7.0 mmol), lithium chloride (0.88 g, 21 mmol), vinyltributyltin (2.66 g, 8.4 mmol), and tetrakis(triphenylphosphine)- palladium(O) (0.243 g, 0.21 mmol) in degassed anhydrous 1 ,4-dioxane (20 mL) was heated at 90 ° for 8 hours. The mixture was then filtered through silica gel (20 g) and concentrated. The residue was redissolved in ether (80 mL), and water (0.2 mL) was added. To this mixture was added DBU (2 mL) dropwise, and then the mixture was filtered through silica gel (20 g). The residue after concentration of the filtrate was purified by column chromatography eluting with 5% ether in hexane (200 mL) followed by 10% ethyl acetate in hexane to give the title compound (1.01 g, 66%) contaminated with small amount of tributyltin derivatives. H NMR (300 MHz, CDCI3) δ 7.75 (d, 1H), 7.71 (d, 1H), 7.55 (dd, 1H), 6.73 (dd, 1H), 5.88 (d, 1H), 5.42 (d, 1H), 3.93 (s, 3H), 3.91 (s, 3H).

Example 98B Dimethyl 4-formyllphthalate A mixture of the compound resulting from Example 98A (1.01 g, 4.6 mmol), osmium tetraoxide (5 mg/mL solution in f-butanol, 10 mL, 0.2 mmol), and sodium periodate (5.74 g, 27 mmol) in 2:1 1 ,4-dioxane-water (30 mL) was stirred 4 hours. The resulting mixture was filtered through celite, rinsed with 1:1 ethyl acetate-ether (90 mL). The filtrate was washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 15% ethyl acetate in hexane to give the title compound (0.948 g, 92%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 10.10 (s, 1 H), 8.60 (d, 1 H), 8.07 (dd, 1 H), 7.84 (d, 1 H), 3.96 (s, 6 H).

Example 98C Dimethyl 4-f3-fpara-phenoxyphenvnpropionvπphthalate

To a solution of 4-phenoxyphenylacetic acid (2.28 g, 10 mmol) in THF (30 mL) was added lithium aluminum hydride (1.0 M in THF, 10 mL) cautiously. After 4 hours at room temperature, the reaction was cooled to 0 °C, and water (0.4 mL), aqueous 15% NaOH (0.4 mL) and water (1.2 mL) were added sequentially. The resulting muddy mixture was filtered through celite and concentrated to give 1(4'-phenoxyphenyl)-1 -ethanol (1.42 g). The alcohol was dissolved in dichloromethane (20 mL), and to this solution was added carbon tetrabromide (2.65 g, 8.0 mmol) in one portion and triphenylphosphine (2.10 g, 8.0 mmol) in 3 equal portions (at 2 minute intervals). After 6 hours, the mixture was diluted with hexane (30 mL), and the mixture was filtered through silica gel (20 g), rinsed with 1:2 ether-hexane (50 mL). The residue after concentration of the filtrate was purified by column chromatography eluting with 5% ethyl acetate in hexane to give 1-(4'-phenoxyphenyl)-2-ethyl bromide (1.67 g, 60%). 1H NMR (300 MHz, CDCI3): δ 7.38-6.95 (m, 9 H), 3.57 (t, 2 H), 3.14 (t, 2 H). A THF (10 mL) solution of the bromide prepared above (1.67 g, 6.02 mmol) in an addition funnel was added to a flask containing magnesium (0.29 g, 12 mmol) and a crystal of iodine. After the reaction was initiated, the addition rate was adjusted so that the reaction was smoothly refluxed. The reaction was

refluxed for 1 hour after completion of the bromide addition to afford the Grignard reagent, 1-(4-phenoxyphenyl)-2-ethylmagnesium bromide. Titration of the solution with n-propanol (1,10-phenantholine as indicator) found that the solution to be 0.55 M. To a solution of the aldehyde prepared in Example 98B (1.35 g, 6.1 mmol) in THF (10 mL) at -78 °C was added slowly the Grignard solution (10 mL, 5.5 mmol). The reaction was warmed to room temperature over 30 minutes and quenched with saturated ammonium chloride (5 mL). The resulting mixture was diluted with ether to 100 mL, washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 30% followed by 50 % ethyl acetate in hexane to give the desired dimethyl 4-[3-(para- phenoxyρhenyl)-1-hydroxypropyl]phthalate as the first fraction (1.32 g, 57%), and dimethyl 4-hydroxymethylphthalate as the second fraction (0.46 g, 34%). 1 H NMR of the desired secondary alcohol (300 MHz, CDCI ₃ ) δ 7.75 (d, 1 H), 7.69 (d, 1H), 7.53 (dd, 1H), 7.32 (t, 2H), 7.15 (d, 2H), 7.09 (dt, 1H), 6.99 (d, 2H),

6.94 (d, 2H), 4.79 (m, 1H), 3.92 (s, 3H), 3.91 (s, 3H), 2.71 (m, 2H), 2.06 (m, 2H),

1.95 (d, 1H).

To a mixture of the above secondary alcohol (1.32 g, 3.14 mmol), activated powdered molecular sieves 4 A (2 g) in dichloromethane (25 mL) at 0 °C was added pyridinium chlorochromate (1.38 g, 6.28 mmol). After 1 hour, the reaction was diluted with ether (25 mL) and filtered through silica gel. Concentration of the filtrate yielded the title compound (1.08 g, 82%). ¹ H NMR (300 MHz, CDCI3) δ 8.31 (d, 1 H), 8.13 (dd, 1H), 7.78 (d, 1H), 7.37-6.92 (m, 9H), 3.95 (s , 6H), 3.33 (t, 2H), 3.07 (t, 2H).

Example 98D 4-f3-Phenyl-2-f4-phenoxybenzvhDropionyl ^' |-1.2-dimethoxycarbonylbenzene To a solution of the compound resulting from Example 98C (491 mg, 1.17 mmol) in THF (10 mL) was added potassium hexamethyldisilazide (0.5 M in toluene, 2.8 mL). After 30 minutes, benzyl bromide (167 μL, 1.41 mmol) was added to the reaction, and the reaction was allowed to warm to room temperature over 15 hours. The reaction was quenched with saturated ammonium chloride (5 mL), diluted with ether to 80 mL, washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 20% ethyl acetate in hexane to give the title compound (470 mg, 79%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.95 (d, 1H), 7.74 (dd, 1H), 7.60 (d, 1H), 7.83-6.82 (m, 9H), 3.95 (m, 1H), 3.92 (s, 3H), 3.91 (s, 3H), 3.10 (m, 2H), 2.88 (dt, 2H).

Example 98E

4-[3-Phenyl-2-f4-phenoxybenzvπpropionvπbenzene-1.2-dica rboxylic acid A mixture of the compound resulting from Example 98D (69 mg) and aqueous 2 N NaOH (1 mL) in THF (3 mL) was refluxed 12 hours. The reaction mixture was then acidified with aqueous 3 M hydrochloric acid (1 mL), diluted with ethyl acetate to 50 mL, washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 95:4:1 ethyl acetate-methanol- formic acid to give the title compound (61.5 mg, 94%). ¹ H NMR (500 MHz, DMSO-de) δ 8.42 (s, 1H), 8.03 (d, 1H), 7.85 (dd, 1H), 7.32-7.06 (m, 10H), 6.80 (d, 2H), 6.78 (d, 2H).4.29 (m, 1 H), 3.04 (dd, 1 H), 2.95 (dd, 1 H), 2.85 (m, 2H). MS (DCl) m/e, 480 (M-H ₂ 0+NH ₄ ⁺ ), 498 (M+NH4 ⁺ ).

Example 99 4-[2-Benzyl-1-hydroxy-3-(4-phenoxyphenvπpropynbenzene-1.2-d icarboxylic acid

Example 99A

4-[2-Benzyl-1 -hvdroxy-3-f4-phenoxyphenyl)lpropyl]-1.2- dimethoxycarbonylbenzene To a solution of the compound resulting from Example 98D (389 mg, 0.76 mmol) in ethanol (5 mL) at 0 °C was added sodium borohydride (58 mg, 1.53 mmol) in two equal portions. The reaction mixture was then stirred at room temperature for 1 hour and poured to a separatory funnel containing ether (80 mL) and half saturated ammonium chloride (10 mL). The orgainc layer was separated and washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 30% ethyl acetate in hexane to give the title compound (311 mg, 79%) as a mixture of diastereomers (-1:1). ¹ H NMR (300 MHz, CDCI3) δ 7.74 (2 doublets, 1H), 7.63 (2 doublets, 1H), 7.48 (m, 1 H), 7.34-6.87 (m, 14H), 4.78 (m, 1H), 3.91 (3 singlets, 6H), 2.70 (m, 2H), 2.58 (m, 2H), 2.32 (m, 1H), 1.89 (d, 1H).

Example 99B 4-r2-Benzyl-1-hvdroxy-3-(4-phenoxyphenvπpropyl1benzene-1.2- dicarboxylic acid

The procedure described in Example 98E and the compound resulting from Example 99A (56 mg, 0.11 mmol) afforded the title compound (50 mg, 94%) as a mixture of 1:1 diastereomers. ¹ H NMR (500 MHz, DMSO-d ₆ ) δ 8.13 (m, 2H), 7.43 (m, 1H), 7.39-6.82 (m, 14H), 4.80 (m, 1H), 2.75 (m, 1H), 2.48 (m, 2H), 2.39 (m, 1 H), 2.25 (m, 1 H). MS (DCl) m/e, 482 (M-H ₂ 0+NH ₄ +), 500 (M+NH4+).

Example 100 4-f2-Benzyl-3-f4-phenoxyρhenvπ-1-propenvπbenzene-1.2-dica rboxylic acid

Example 100A Dimethyl (cis & fraπsV4-f2-benzyl-3-f4-phenoxyphenvn-proDen-1-yl1phthalatfi To a solution of the compound resulting from Example 99A (208 mg, 0.41 mmol) and pyridine (0. 6 mL) in dichloromethane (5 mL) at 0 °C was added phosphorus oxychloride (0.3 mL) dropwise, follwed by DBU (0.2 mL). After the reaction mixture was stirred at room temperature for 2 hours, it was diluted with 1:1 ether-hexane (20 mL), filtered through silica gel, and rinsed with ether. The residue after concentration of the filtrate was purified by column chromatography eluting with 20% ethyl acetate in hexane to give the title compound (31 mg, 15%) as a mixture of 1:1 trans/cis isomers. ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.75-7.47 (m, 3H), 7.36-6.88 (m, 14H), 4.98 (m, 1H), 3.92 (3 singlets, 6H), 2.66 (m, 4H).

Example 100B (cis & trans 4-f2-Benzyl-3-f4-phenoxyphenyπ-1-propenvnbenzene-1.2- dicarboxylic acid The procedure decribed in Example 98E and the compound resulting from Example 100A (30 mg) afforded the title compound (13 mg, 51%) as a mixture of 1:1 trans/cis isomers. ¹ H NMR (300 MHz, DMSO-dβ) δ 7.80-6.83 (m, 17H), 5.59 & 4.61 (2 doublets, 1H), 2.80-2.30 (m, 4H). MS (DCl) m/e, 464 (M- H2O+NH4+), 482 (M+NH ₄ ⁺ ).

Example 101 4-[N-Benzyl-N-f4-phenoxybenzvπaminomethyl1benzene-1.2-dicar boxyIic acid

Example 101 A Dimethyl 4-fN-benzyl-N-rpara-Dhenoxy)benzylaminomethvnphthalate

A solution of the compound resulting from Example 98B (228 mg, 1.02 mmol), N-benzyl-N-(4-phenoxy)benzylamine hydrochloride (332 mg, 1.02 mmol), sodium cyanoborohydride (1.0 M in THF, 1.0 mL), and acetic acid (0.3 mL) in ethanol was stirred at room temperature for 15 hours. The reaction mixture was then diluted with ethyl acetate (80 mL), washed with 15% aqueous NaOH (10 mL), saturated sodium bicarbonate (10 mL), water (10 mL), and brine, dried over anhydrous potassium carbonate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography to give the title compound (510 mg, 100%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.72 (d, 1H), 7.70 (s, 1H), 7.59 (d, 1H), 7.40-6.95 (m, 14H), 3.92 (s, 3H), 3.90 (s, 3H), 3.61 (s, 1H), 3.56 (s, 2H), 3.52 (s, 2H).

Example 101B 4-[N-Benzyl-N-(4-phenoxybenzvπaminomethyl]benzene-1.2-dicar boxylic acid A mixture of the compound resulting from Example 101 A (512 mg, 1.03 mmol), aqueous 2 N NaOH (2 mL) in THF (5 mL) was refluxed 12 hours. To the reaction mixture was added 4 N hydrogen chloride in 1 ,4-dioxane (2 mL) and silica gel (1 g), and the mixture was evaporated to dryness. The residue was suspended in chloroform, and the slurry was loaded to a column packed with silica gel. The column was eluted sequentially with 3% methanol in ethyl acetate followed by 94:5:1 ethyl acetate-methanol-formic acid to give the title compound (458 mg, 88%). ¹ H NMR (500 MHz, DMSO-d ₆ ) δ 11.90 (br singlet, 2H), 9.95 (br. singlet 1H), 7.90 (m, 2H), 7.63 (m, 5H), 7.15 (t, 1H), 7.00 (d, 2H), 6.93 (d, 2H). MS (DCl) m/e, 468 (M+H)+, 450 (M-H ₂ 0+H)+.

Example 102a 3-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonyn-6-formyl-4-meth oxybenzoic acid and Example 102b 4-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonvn-5-methoxybenzen e-1.2- dicarboxylic acid

Example 102A Methyl 5-bromo-4-hvdroxy-2-methoxybenzoate To a solution of 5-bromo-2,4-dihydroxybenzoic acid monohydrate (2.76 g, 11 mmol) in methanol (5 mL) and ethyl adetate (5 mL) at 0 °C was added 2.0 M trimethylsilyI)diazomethane in hexane (6 mL). The reaction was then moved to a 40 °C water bath, and additional (trimethylsilyl)diazomethane was added slowly, until the yellow color was sustained at that temperature over 5 minutes. The solvent was then evaporated to give the title compound as a white solid (2.84 g, 99%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 10.94 (s, 1H), 7.99 (s, 1 H), 6.49 (s, 1H), 3.93 (s, 3H), 3.91 (s, 3H).

Example 102B Methyl 5-bromo-2-methoxy-4-trifluoromethanesulfonyloxybenzoate

To a solution of the compound resulting from Example 102A (2.84 g, 10.9 mmol) in pyridine (5 mL) and dichloromethane(30 mL) at -78 °C was slowly added trifluoromethanesulfonic anhydride (2.22 mL, 13.2 mmol). After the reaction mixture was warmed to room temperature over 15 hours, it was diluted with ether (80 mL), washed with water, saturated copper(ll) sulfate and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 10% ethyl acetate in hexane to give the title compound (3.51 g, 84%). 1H NMR (300 MHz, CDCI3) δ 8.31 (s, 1 H), 6.75 (s, 1 H), 3.98 (s, 3 H), 3.96 (s , 3 H).

Example 102C Methyl 2-methoxy-4.5-divinylbenzoate A mixture of the compound resulting from Example 102B (1.15 g, 3.0 mmol), vinyltributyltin (2.38 g, 7.5 mmol), lithium chloride (0.64 g, 15 mmol), tetrakis(triphenylphosphine)palladium(0) (0.173 g, 0.15 mmol) in nitrogen degassed anhydrous 1,4-dioxane (15 mL) was heated at 98 °C for 6 hours. The mixture was then filtered through silica gel (10 g) and then concentrated. The residue was redissolved in ether (40 mL), and water (0.2 mL) was added. To this mixture was added DBU (0.5 mL) dropwise, and the mixture was filtered through silica gel (20 g). The residue after concentration of the filtrate was purified by column chromatography eluting with hexane (200 mL) followed by 5% ethyl acetate in hexane to give the title compound (370 mg) contaminated with small amount of tributyltin derivatives. ¹ H NMR (300 MHz, CDCI3) δ 8.07 (s, 1H), 7.56 (dd, 1H), 7.01 (s, 1H), 6.98 (dd, 1H), 5.84 (dd, 1H), 5.65 (dd, 1H), 5.34 (m, 2H), 3.94 (s, 3H), 3.89 (s, 3H).

Example 102D 2-Methoxy-4.5-divinylbenzoic acid A mixture of the compound resulting from Example 102C (370 mg) and aqueous 2 N NaOH (2 mL) in THF (6 mL) was refluxed for 7 hours. The reaction mixture was then acidified with aqueous 3 N hydrochloric acid (2 mL), diluted with ethyl acetate to 100 mL, washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 10% ether in hexane followed by 100% ether to give the title compound (341 mg, 56%, 2 steps). 1H NMR (300 MHz, CDCI ₃ ) δ 8.19 (s, 1H), 7.65 (dd, 1H), 7.02 (s, 1H), 6.98 (dd, 1H), 5.84 (d, 1H), 5.66 (d, 1H), 5.48 (d, 1H), 5.44 (d , 1H), 3.97 (s, 3H).

Example 102E N-Benzyl-N-(4-Dhenoxybenzvπ-2-methoxy-4.5-divinylbenzamide To a solution of the compound resulting from Example 102D (331 mg, 1.6 mmol) in dichloromethane (5 mL) was added oxalyl chloride (2.0 M, 1.6 mL), followed by a tiny drop of DMF. After 1 hour at room temperature, the solvent was evaporated, and the residue was attached to high vacuum line (1 mm Hg) for 30 minutes. The residue was then redissolved in dichloromethane (10 mL), and to it was added N-benzyl-N-(4-phenoxybenzyl)amine (509 mg, 1.76 mmol) and 4-dimethylaminopyridine (10 mg). After 1 hour, the reaction mixture was diluted with ether (100 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 15% ethyl acetate in hexane to give the title compound (531 mg, 70%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.40-6.75 (m, 18H), 5.77-5.54 (m, 2H), 5.33-5.20 (m, 2H), 4.28- 4.19 (m, 4H), 3.89 (2 singlets, 3H).

Example 102a 3- N-Benzyl-N-f4-phenoxybenzvπaminocarbonvn-6-formyl-4-methoxy benzoic acid and Example 102b

4-[N-Benzyl-N-(4-phenoxybenzvπaminocarbonyl]-3-methoxybe nzene-1.2- dicarboxylic acid A mixture of the compound resulting from Example 102E (523 mg, 1.10 mmol), ruthenium(lll) chloride monohydrate (11.4 mg, 0.055 mmol), and sodium periodate (3.53 g, 16.5 mmol) in 1 :1:1.5 acetonitrile-carbon tetrachloride-water (21 mL) was stirred at room temperature for 6 hours. The reaction mixture was then filtered through celite and rinsed with ethyl acetate (100 mL). The filtrate was then washed with 0.1 N aqueous HCI (15 mL), water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 3% methanol in ethyl acetate followed by 94:5:1 ethyl acetate-methanol-formic acid to give 3- [N-benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-6-formyl-4-metho xybenzoic acid (102a) as the first fraction (52 mg, 9.6%), and 4-[N-benzyl-N-(4-

phenoxybenzyl)aminocarbonyl]-3-methoxybenzene-1 ,2-dicarboxylic acid (102b) as the second fraction (171 mg, 30%).

1H NMR (500 MHz, DMSO-d ₆ ) for benzoic acid 102a: δ 9.98 (2 singlets, 1H), 7.63 (3 singlets, 2H), 7.46-6.85 (m, 14H), 4.73 & 4.67 (2 singlets, 2H), 4.32 and 4.28 (2 singlets, 2H), 3.91 (s, 3H). MS (FAB -) m/e 494 (M-H).

1H NMR (500 MHz, DMSO-d ₆ ) for phthalic acid 102b: δ 7.55 (4 singlets, 2H), 7.42-6.85 (m, 14H), 4.25-4.05 (4 singlets, 4H), 3.88 (2 singlets, 3H). MS (FAB+) m/e, 512 (M+H), 534 (M+Na), 550 (M+K).

Example 103

5-rN-Benzyl-N-f4-phenoxybenzyπaminocarbonvn-4-hydroxyben zene-1.2- dicarboxylic acid A solution of the compound resulting from Example 102b (83 mg, 0.16 mmol), boron tribromide methyl sulfide complex (1.0 M. in CH2CI2, 1.6 mL) in dichloromethane (5 mL) was stirred 15 hours at room temperature. The reaction was then quenched with saturated ammonium chloride (2 mL), diluted with half saturated brine (10 mL), and extracted with 1:4 methanol-chloroform (4 x 10 mL). The combined organic extracts were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 5% methanol in ethyl acetate followed by 94:5:1 ethyl acetate-methanol-formic acid to give the title compound (53 mg, 67%). 1H NMR (500 MHz, DMSO-d ₆ ) δ 7.67-6.87 (m, 16 H), 4.26-4.17 (4 singlets, 4 H). MS (FAB +) m/e, 498 (M+H)+, 520 (M+Na)+.

Example 104

2-| ^" N-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1-5-methoxy-1 .4- dimethoxycarbonylbenzene

Example 104A

Methyl 4-hvdroxy-2-methoxy-5-vinylbenzoate A solution of the compound resulting from Example 102A (5.66 g, 20 mmol), vinyltributyltin (6.97 g, 22 mmol) tetrakis(triphenylphosphine)palladium (0.462 g, 0.40 mmol) in nitrogen degassed anhydrous toluene (80 mL) was heated at 108 °C for 10 hours. The mixture was then filtered through silica gel (10 g) and then concentrated. The residue was redissolved in ether (40 mL), and water (0.2 mL) was added. To this mixture was added DBU (1 mL) dropwise, and the mixture was filtered through silica gel (20 g). The residue after concentration of the filtrate was purified with column chromatography eluting with hexane (200 mL) followed by 5% ethyl acetate in hexane to give the title compound (2.62 g, 63%) contaminated with small amount of tributyltin derivatives. 1H NMR (300 MHz, CDCI3) δ 10.99 (s, 1H), 7.91 (s, 1H), 6.88 (dd, 1H), ^' 6.44 (s, 1H), 5.66 (dd, 1H), 5.19 (dd, 1H), 4.94 (s , 3H), 3.87 (s, 3H).

Example 104B

1-Carbomethoxy-2-methoxy-5-vinyl-4-tr?fluoromethanesulfon yloxybenzene The procedure described in Example 102B and the compound resulting from Example 104A (2.86 g, 13.7 mmol) afforded the title compound (3.04 g, 65%). ¹ H NMR (300 MHz, CDCI3) δ 8.18 (s, 1H), 6.94 (dd, 1H), 6.72 (s, 1H), 5.86 (dd, 1 H), 5.42 (dd, 1 H), 3.95 (s, 3H), 3.92 (s, 3H).

Example 104C 1-Carbomethoxy-2-methoxy-5-fN-benzyl-N-f4-phenoxybenzvπamin ocarbonyl]- 4-trifluoromethanesulfonyloxybenzene A mixture of the compound resulting from Example 104B (1.92 mg, 5.64 mmol), ruthenium(III) chloride monohydrate (60 mg, 0.28 mmol), and sodium periodate (6.03 g, 28.2 mmol) in 1:1:1.5 acetonitrile-carbon tetrachloride-water (35 mL) was stirred at 40 °C for 3 hours. The reaction mixture was then filtered through celite and rinsed with ethyl acetate (100 mL). The filtrate was then washed with 0.1 aqueous HCI (15 mL), water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The crude acid was used without further purification. ¹ H NMR (300 MHz, DMSO-dβ) δ 8.43 (s, 1H), 7.27 (s, 1H), 3.97 (s, 3H), 3.87 (s, 3H).

Half of the crude acid was dissolved in THF (10 mL), and to it was added 1-(3-dimethylaminopropyl)-3-ethyIcarbodiimide hydrochloride (704 mg, 3.67 mmol), N-benzyl-N-(4-phenoxybenzyl)amine (897 mg, 3.10 mmol), and 4- dimethylaminopyridine (50 mg). After 6 hours at room temperature, the reaction mixture was diluted with ether (100 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 20% ethyl acetate in hexane to give the title compound (0.887 g, 50%, 2 steps). ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.05 (2 singlets, 1H), 7.40-6.76 (m, 15H), (2 br. multiplets, 2H), 4.23 & 4.18 (2 singlets, 2H), 3.98-3.67 (4 singlets, 6H).

Example 104D 2-rN-Benzyl-N-f4-phenoxybenzyl)aminocarbonyl ^' |-5-methoxy-1.4- dimethoxycarbonylbenzene A carbon monoxide degassed mixture of the compound resulting from Example 104C (867 mg, 1.38 mmol), palladium(ll) acetate (16 mg, 0.07 mmol), 1 ,3-bis(diphenylphosphino)propane (29 mg, 0.07 mmol) and triethylamine (281 mg, 2.76 mmol) in methanol (2 mL) and DMF (5 mL) was heated to 65 °C under a carbon monoxide balloon for 15 hours. The reaction mixture was diluted with 1:1 ether-ethyl acetate (100 mL), washed with 10% aqueous HCI, water, and brine, dried over anhydrous magnesium sulfate, filtered, and

O 96/34851

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concentrated in vacuo. The residue was then purified by column chromatography eluting with 30% ethyl acetate in hexane to give the title compound (631 mg, 85%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.35 (2 singlets, 1H), 7.40-6.92 (m, 15H), 4.90 and 4.50 (2 br. multiplets, 2H), 4.22 and 4.18 (2 singlets, 2H), 3.98-3.67 (5 singlets, 9H). MS (DCl) m/e, 540 (M+H)+.

Example 105 2-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonvπ-5-methoxybenze ne-1.4- dicarboxylic acid The procedure described in Example 98E and the compound resulting from Example 104 (574 mg, 1.06 mmol) afforded the title compound (517 mg, 95%). ¹ H NMR (300 MHz, DMSO-de) δ 7.93 ( 2 singlets, 1H), 7.63 (2 singlets, 1H), 7.43-6.90 (14H), 4.92-4.78 and 4.45-4.15 (m, 4H), 3.92 and 3.90 (2 singlets, 3H). MS (FAB-) m/e 510 (M-H).

Example 106 2-fN-Benzyl-N- 4-phenoxybenzvπaminocarbonvn-5-hvdroxybenzene-1.4- dicarboxylic acid A solution of the compound resulting from Example 105 (200 mg, 0.39 mmol), boron tribromide methyl sulfide complex (1.16 g, 3.7 mmol) in anhydrous 1 ,2-dichloroethane was refluxed for 50 hours. The reaction was then quenched with saturated sodium bicarbonate, diluted with ethyl acetate (100 mL), washed with 10% aqueous HCI, water and brine, dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 94:5:1 ethyl acetate- methanol-formic acid to give the title compound (135 mg, 70%). ¹ H NMR (300 MHz, DMSO-de) δ 10.94 (br. s, 1H), 9.5 (m, 1H), 8.54 (br. s, 1H), 7.80 (br. s, 1H), 7.42-6.88 (m,14H), 4.65-4.50 and 4.30 (6 br. singlets, 4H). MS (FAB -) m/e 496 (M-H).

Example 107

4-[N-Benzyl-N-(4-phenoxybenzyl)aminocarbonyl]-6-methoxy-1 .3- dimethoxycarbonylbenzene

Example 107A

Dimethyl 4-triflyloxy-6-methoxy-1.3-isophthalate A mixture of the compound resulting from Example 104B (1.10 g, 3.23 mmol), ruthenium chloride monohydrate (32 mg, 0.16 mmol), and sodium periodate (3.51 g, 16.2 mmol) in 1:1:1.5 acetonitrile-carbon tetrachloride-water (28 mL) was stirred at 45 ^C C for 6 hours. The reaction mixture was then filtered through celite and rinsed with ethyl acetate (100 mL). The filtrate was then washed with 0.1 N aqueous HCI (15 mL), water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was dissolved in 3:1 ethyl acetate-methanol (10 mL), cooled in a 0 °C water bath, and treated with (trimethylsilyl)diazomethane (2.0 M in hexane) in small portions until the yellow color persisted. The solvent was evaporated, and the residue was purified by column chromatography eluting with 30% ethyl acetate in hexane to give the title compound (915 mg, 76%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.58 (s, 1H), 6.83 (s, 1H), 3.98 (s, 3H), 3.96 (s, 3H), 3.93 (s , 3H).

Example 107B

Dimethyl 6-methoxy-4-vinyl-1.3-isophthalate The procedure described in Example 102C, the compound resulting from Example 107A (905 mg, 2.43 mmol) and tributylvinyltin (849 mg, 2.68 mmol) afforded the title compound (114 mg, 19%). ¹ H NMR (300 MHz, CDCI3) δ 8.47 (s, 1 H), 7.60 (dd, 1 H), 7.10 (s, 1 H), 5.71 (d, 1 H), 5.47 (d, 1 H), 4.01 (s, 3H), 3.91 (s, 3H), 3.89 (s, 3H).

Example 107C 4-fN-Benzyl-N-(4-phenoxybenzvπaminocarbonvπ-6-methoxy-1.3- dimethoxycarbonylbenzene A mixture of the compound resulting from Example 107B (103 mg, 0.412 mmol), ruthenium(lll) chloride monohydrate (5 mg), and sodium periodate (0.5 g) in 1:1:1.5 acetonitrile-carbon tetrachloride-water (7 mL) was stirred at 60 °C for 6 hours. The reaction mixture was then filtered through celite and rinsed with ethyl acetate (50 mL). The filtrate was then washed with 0.1 N aqueous HCI (5 mL) and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The crude dimethyl 4-[N-benzyl-N-(4- phenoxybenzyl)aminocarbonylJ-6-methoxy-1 ,3-isophthalate was used without further purification. H NMR (300 MHz, CDCI3) δ 8.18 (s, 1 H), 7.35 (s, 1 H), 3.97 (s , 3 H), 3.89 (s, 3 H), 3.87 (s, 3 H).

The crude acid from above was reacted by the procedures described in Example 102E to give the title compound (217 mg, 98%, 2 steps). 1H NMR (300 MHz, CDCI3) δ 8.50 (2 singlets, 1H), 7.50-6.84 (m, 15H), 4.25 and 4.19 (2 br. singlets, 4H), 3.90-3.74 ( 6 singlets, 9H). MS (DCl) m/e 540 (M+H)+.

Example 108 4-rN-Benzyl-N-f4-phenoxybenzyhaminocarbonyl]-6-methoxy-benze ne-1.3- dicarboxylic acid The procedure described in Example 98E and the compound resulting from Example 107C (143 mg, 0.26 mmol) afforded the title compound (32 mg, 24%). 1H NMR (300 MHz, DMSO-de) δ 8.0 (br. s, 1 H), 7.77 (br. s, 1 H), 7.43- 6.90 (m, 14H), 4.83-4.15 (m, 4H), 3.91 and 3.89 (2 singlets, 3H). MS (FAB -) m/e 510 (M-H).

Example 109 4-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1-6-hvdroxy-benz ene-1.3- dicarboxylic acid The procedure described in Example 106 and the compound resulting from Example 107 (162 mg, 0.30 mmol) afforded the title compound (79 mg, 53%). 1H NMR (300 MHz, DMSO-d ₆ ) δ 13.03 (br. s, 1H), 11.09 (2 singlets, 1H), 9.52 (m, 1H), 8.38 (2 singlets, 1H), 7.43-6.82 (m, 15H), 4.95-4.15 (m, 4H). MS (FAB-) m/e (M-H), 496.

Example 110

5-fN-Benzyl-N-(4-phenoxybenzvπaminocarbonyl]-1.3- dimethoxycarbonylbenzene

Example 110A 3.5-Dimethoxycarbonylbenzoic acid

To a stirred solution of 1 ,3,5-trimethoxycarbonylbenzene (2.52 g, 10 mmol., 1.0 eq.) in 40 mL of 3:1 methanol-THF at ambient temperature was added a solution of 0.64 g (10 mmol, 1.0 eq.) of KOH in 6 mL of methanol, and the resulting solution was stirred overnight. The slurry formed was then concentrated and partioned between ethyl ether and water. The aqueous phase was extracted with an additional portion of ethyl ether and then acidified with concentrated aqueous HCI. The resulting suspension was extracted with 2 portions of ethyl acetate, and the combined organic phases were washed with water and brine, dried, filtered and concentrated to give a white solid that was used without further purification. ¹ H NMR (300 MHz, CD ₃ OD) δ 8.76-8.84 (m, 3H), 3.97 (s, 6H). MS (DCl, NH ₃ ): 273 (35%); 256 (100%); 212 (10%).

Example 11 OB 3.5-Bisfmethoxycarbonyl)benzoyl chloride To a stirred suspension of 476 mg (2 mmol, 1 eq.) of the compound resulting from Example 110A in 20 mL of CH2CI2 at room temperature was added 0.21 mL (2.4 mmol, 1.2 eq.) of oxalyl chloride dropwise via syringe. After the addition was complete, 2 drops of dry DMF were added and the mixture stirred for 1.5 hours. To the yellow solution was added 5 mL of toluene, and the mixture was concentrated to give 510 mg (99%) of the title compound as an off white solid that was used without further purification.

Example 11 PC 5-[N-Benzyl-N-4-phenoxybenzylaminocarbonyll-1.3- dimethoxycarbonylbenzene To a stirred solution of 342 mg (1.05 mmol, 1.05 eq.) of N-benzyl-N-(4- ρhenoxybenzyl)amine-HCI in 5 mL of CH2CI2 was added 0.31 mL (2.2 mmol, 2.2 eq.) of triethylamine. To the resulting solution was added 256 mg (1.0 mmol, 1.0 eq.) of the compound resulting from Example 110B dissolved in 5 mL of CH2CI2 dropwise, and stirring was continued for 2 hours at room temperature. The mixture was poured into 50 mL of hexanes and extracted with 2 portions of water, 2 portions of 3 N aqueous HCI, 1 portion of brine and then dried, filtered and concentrated. The residue was purified by column chormatography on Siθ2 (25 g) eluting with 15-25% ethyl acetate in hexanes to give 326 mg (64%) of the title compound as a thick syrup. ¹ H NMR (300 MHz., CDCI3) δ 8.70 (t, 1H), 8.33 (d, 2H), 7.36 (m, 7H), 7.13 (m, 2H), 7.01 (m, 5H), 4.54 (broad AB quartet, 4H), 3.92 (s, 6H). MS (DCl, NH ₃ ) m/e 527 (100%); 510 (78%). HRMS (FAB+) calcd for C ₃ ιH ₂ 8NOe (M+H) 510.1917. Found: 510.1903. Anal calcd for C31H27NO6 ^• 0.19 H ₂ 0: C, 73.07; H, 5.34; N, 2.75. Found: C, 72.58; H.5.26; N, 2.62.

Example 111 5-fN-Benzyl-N-4-phenoxybenzylaminocarbonyl]-3-methoxycarbony lbenzoic acid To a stirred solution of 347 mg (0.68 mmol, 1.0 eq.) of the compound resulting from Example 110 in 9 mL of THF at -10 °C was added a solution of 29 mg (0.68 mmol, 1.0 eq.) of LiOH in 3 mL of H2O. Methanol was add'jd until a clear solution was obtained. The bath was replaced after 1 hour with an ice water bath and the mixture allowed to stir overnight during which time the ice bath melted. The mixture was quenched with excess aqueous HCI and poured into 3 N aqueous HCI. The suspension was extracted with 2 portions of ethyl acetate, and the combined organic phases were washed with brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (25 g) eluting with 98:1.5:0.5 going to 90:10:1 CHCl3-methanol-acetic acid to give 93 mg (27%) recovered diester and 185 mg (55%) of the title compound as a white foam. ^" "H NMR (300 MHz, CDCI3) δ 8.76 (t, 1H), 8.37 (d, 2H), 7.34 (m, 7H), 7.11 (m, 2H), 7.01 (m, 5H), 4.53.(broad AB quartet, 4H), 3.92 (s, 3H). MS (DCl NH ₃ ): 513 (50%); 496 (55%); 404, (10%); 273 (18%); 256 (78%); 241 (100%); 200 (60%); 183 (83%). Anal calcd for C30H25NO6 ^• 0.36 H ₂ 0: C, 72.72; H, 5.08; N, 2.83. Found: C, 71.78; H, 5.17; N, 3.20.

Example 112 5-rN-Benzyl-N-4-phenoxybenzylaminocarbonyl]benzene-1.3-dicar boxylic acid To a stirred solution of 322 mg (0.63 mmol, 1.0 eq.) of the compound resulting from Example 111 in 9 mL of THF was added a solution of 79 mg (1.90 mmol, 3 eq.) of LiOH in 3 mL of H2O, and the solution was stirred overnight. The basic mixture was quenched with 3 aqueous HCI and partioned between 3 N aqueous HCI and 2 portions of ethyl acetate. The combined organic phases were washed with brine, dried, filtered and concentrated. The residue was lyophilized to give 282 mg (91%) of the title compound as a fluffy white solid. ¹ H NMR (300 MHz., CD3OD) δ 8.68 (t, 1H), 8.24 (t, 2H), 7.34 (m, 7H), 7.12 (m, 2H), 6.97 (m, 5H), 4.47 (m, 2H), 2 protons buried under MeOH peak. MS (FAB+): 482 (60%); 460 (18%); 307 (23%); 289

(18%); 183 (15%); 154 (100%); 136 (70%). Anal calcd for C29H23NO6 • 0.98 H ₂ 0: C, 72.34; H, 4.81; N, 2.91. Found: C, 69.78; H, 4.99; N, 2.69.

Example 113 5-[N-Benzyl-N-4-phenoxybenzylaminocarbonvπ-3-carboxamidoben zoic acid

Example 113A

5-fN-Benzyl-N-4-phenoxybenzylaminocarbonvn-3-fN- benzyloxyaminotearbonylbenzoic acid methvl ester To a stirred solution of the compound resulting from Example 112 (50 mg, 0.10 mmol, 1.0 eq.) in 1 mL of THF at -10 °C was sequentially added 12 μL (0.11 mmol, 1.1 eq.) of 4-methylmorρholine and 14 μL (0.11 mmol, 1.1 eq.) of isobutylchloroformate and stirring was continued for 30 minutes. To the resulting white suspension was added 19 mg (0.12 mmol, 1.2 eq.) of O- benzylhydroxylamine hydrochloride followed by 13 μL of N-methylmorpholine and stirring was continued overnight . The reaction mixture was partitioned between ethyl acetate and H2O. The aqueous phase was extracted with an additional portion of ethyl acetate and the combined organic phases were washed with 3 H aqueous HCL and brine, dried, filtered and concentrated. Column chromatography on Siθ2 (15 g) eluting with 40% ethyl acetate in hexanes gave 45 mg (75%) of the title compound as a thick syrup. ¹ H NMR (300 MHz., CDCI3) δ 8.31 (s, 1H), 8.24 (s, 1 H), 7.93 (bd, 1H), 7.25-7.46 (m, 12H), 6.93-7.17 (m, 7H), 5.02 (bs, 2H), 4.51 (broad AB quartet, 4H), 3.92 (s, 3H). MS (DCl, NH3): 618 (100%); 601 (100%); 512 (62%); 495 (30%); 361 (47%); 255 (38%); 213 (77%).

Example 113B 5-fN-Benzyl-N-4-phenoxybenzylaminocarbonyl1-3-carboxamidoben zoic acid To a stirred solution of 42 mg (0.07 mmol, 1.0 eq.) of the compound resulting from Example 113A in 0.75 mL of THF was added a solution of 6 mg (0.14 mmol 2.0 eq.) of UOH-H2O in 0.25 mL of H2O. The mixture was stirred overnight and quenched by the addition of 3 ϋ aqueous HCI and poured into a separatory funnel containing 3 aqueous HCI and ethyl acetate. The layers

were separated and the aqueous layer was extracted with 2 additional portions of ethyl acetate. The combined organic phases were washed with brine, dried, filtered and concentrated. The residue was dissolved in 3 mL of ethanol and the solution purged with N2. To this solution was added 40 mg of 10% Pd/C and the suspension stirred under a balloon of H2 for 20 hours. The mixture was filtered through a pad of celite, washed with ethanol and concentrated to give 23 mg of the title compound as a white foam. ¹ H NMR (300 MHz., DMSO-dβ) δ 8.48 (s, 1H), 8.24 (s, 1H), 8.13 (s, 1H), 8.02 (s, 1H), 7.51 (s, 1H), 7.34 (m, 7H), 7.12 (m, 2H), 6.98 (m, 5H), 4.66 (bd, 2H), 4.43 (bs, 2H). MS (DCl NH ₃ ) m/e 498 (100%); 481 (40%). MS (FAB+): 503 (M+Na ⁺ , 18%); 481 (M+H+, 80%); 460 (45%); 307 (25%); 154 (100%); 136 (63%). MS (FAB-): 479 (M-H, 60%); 459 (42%); 306 (38%); 199 (30%); 168 (40%); 153 (100%); 122 (18%). Anal calcd for C29H24N2O5 • 1.54 H ₂ 0: C, 72.49; H, 5.03; N, 5.83. Found: C, 68.54; H, 5.02; N, 5.20.

Example 114 3-rN-Benzyl-N-4-phenoxybenzylaminocarbonyl]-5-hydroxymethylb enzoic acid

Example 114A 3-| ^" N-Benzyl-N-4-phenoxybenzylaminocarbonvπ-5-hvdroxymeth ylbenzoic acid methyl ester To a stirred solution of 495 mg (1.0 mmol. 1.0 eq.) of the compound resulting from Example 111 in 5 mL of THF at 0 °C was added 0.12 mL (1.1 mmol, 1.1 eq.) of N-methylmorpholine. After 30 minutes of stirring, 0.14 mL (1.05 mmol, 1.05 eq.) of isobutylchloroformate was added dropwise and stirring was continued for an additional 30 minutes. To this suspension was added 227 mg (6.0 mmol, 6.0 eq.) of NaBH4 followed immediately by the addition of 1 mL of saturated aqueous NaHCθ3 solution, and the mixture was stirred for 1 hour more and quenched by the addition of 0.5 M H3PO4. The reaction mixture was partitioned between ethyl acetate (2 x 20 mL) and 0.5 M aqueous H3PO4 (10 mL). The combined organic phases were washed with brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (25 g) eluting with 1:1 ethyl acetate-hexanes to give 409 mg (85%) of the

title compound as a white foam contaminated with a small amount of an unknown impurity. ^" -H NMR (300 MHz. CDCI3) δ 8.06 (s, 2H), 7.18 (s, 1 H), 7.37 (m, 7H), 7.13 (m, 2H), 7.02 (m, 5H), 4.74 (m, 4H), 4.39 (d, 2H), 3.91 (s, 3H). MS (DCl, NH ₃ ) m/e 482 (100%).

Example 114B 3-fN-Benzyl-N-4-phenoxybenzylaminocarbonvn-5-hvdroxymethylbe nzoic acid

To a stirred solution of the compound resulting from Example 114A (32 mg, 0.066 mmol, 1.0 eq.) in 1.5 mL of THF was added a solution of 6 mg (0.14 mmol, 2.0 eq.) of UOH-H2O in 0.5 L of H2O and stirring was continued overnight. The mixture was quenched by the addition of 3 aqueous HCI and partitioned between ethyl acetate and 3 aqueous HCI. The aqueous phase was extracted with an additional portion of ethyl acetate, and the combined organic phases were washed with brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (15 g) eluting with 97:2:1 going to 94:5:1 CHCl3-methanol-acetic acid and subsequently lyophilized to give 30 mg (97%) of the title compound as a fluffy lyophilate. ¹ H NMR (300 MHz. CDCI3) δ 8.06 (s, 1H), 8.05 (s, 1H), 7.65 (s, 1H), 7.34 (m, 7H), 7.12 (m, 2H), 7.01 (m, 5H), 4.71 (m, 4H), 4.36 (d, 2H). MS (DCl, NH ₃ ) m/e 485 (63%); 468 (100%). HRMS (FAB+): calcd for C29H26NO5 (MH+): 468.1811. Found: 468.1808.

Example 115 3- ^r N-Benzyl-N-4-Dhenoxybenzylaminocarbonvπ-5-formylbenzo ic acid

Example 115A 3-fN-Benzyl-N-4-phenoxybenzylaminocarbonvπ-5-formylbenzoic acid methyl ester To a stirred solution of the 151 mg (0.31 mmol, 1.0 eq.) of the compound resulting from Example 114 in 5 mL of 10% acetonitrile in CH2CI2 was added 54 mg (0.46 mmol, 1.6 eq.) of N-methylmorpholine followed by 300 mg of active 4A molecular sieves, and stirring was continued for 15 minutes. To this suspension was added 6 mg (0.016 mmol, 0.05 eq.) of TPAP and the mixture

stirred for an additional 45 minutes during which time it turned from green to black. To the suspension was added celite (~1 g) and 15 mL of ethyl ether, and vigorous stirring was continued for 2 minutes, and then the suspension was filtered through a 1/2" X 1" pad of Siθ2 (prewetted with ether) and washed generously with ether. The filtrate was concentrated and the residue purified by column chromatography on Siθ2 (20 g) eluting with 35% ethyl acetate in hexanes to give 103 mg (69%) of the title compound as a white foam. ¹ H NMR (300 MHz., CDCI ₃ ) δ 10.04 (s, 1H), 8.55 (t, 1H), 8.39 (t, 1H), 8.17 (t, 1H), 7.37 (m, 7H), 7.11 (m, 2H), 7.02 (m, 5H), 4.72 (bd, 2H), 4.38 (bd, 2H), 3.95 (s, 3H). MS (DCl, NH ₃ ) m/e 497 (98%); 480 (100%).

Example 115B 3-fN-Benzyl-N-4-phenoxybenzylaminocarbonyl1-5-formylbenzoic acid Using the procedure of Example 114B and the compound resulting from Example 115A, the title compound was prepared. ¹ H NMR (300 MHz., CDCI3) δ 10.04 (s, 1H), 8.61 (s, 1H), 8.44 (t, 1H),*8.21 (s, 1H), 7.38 (m, 7H), 7.13 (m, 2H), 7.02 (m, 5H), 4.74 (d, 2H), 4.40 (d, 2H). MS (DCl, NH3) m/e 483 (75%); 466 (100%). HRMS (FAB+): calcd for C29H24NO5: 466.1654. Found: 466.1655.

Example 116

3-fN-Benzyl-N-4-phenoxybenzylaminocarbonyl]-5-fN- hydroxyiminoformvπbenzoic acid

Example 116A

3-[N-Benzyl-N-4-phenoxybenzylaminocarbonyl ^' |-5-fN- hvdroxyiminoformyObenzoic acid methyl ester To a stirred solution of 200 mg (0.42 mmol, 1.0 eq.) of the compound resulting from Example 115A in 4 mL of methanol was sequentially added 41 mg (0.50 mmol, 1.2 eq.) of sodium acetate and 35 mg (0.50 mmol, 1.2 eq.) of hydroxylamine hydrochloride. After stirring for an additional hour, the mixture was partitioned between ethyl acetate and H2O. The organic phase was dried, filtered and concentrated to give 200 mg (99%) of the title compound as a white

foam. 1H NMR (300 MHz., CDCI ₃ ) δ 8.23 (t, 1H), 8.12 (t, 1 H), 7.94 (t, 1 H), 7.63 (s, 1H), 7.36 (m, 7H), 7.13 (m, 2H), 7.01 (m, 5H), 4.72 (m, 2H), 4.39 (m, 2H), 3.92 (s, 3H). MS (DCl, NH ₃ ) m/e 495 (60%); 213 (100%).

Example 116B

3-rN-Benzyl-N-4-phenoxybenzylaminocarbonvπ-5- ⁽ N- hydroxyiminoformvflbenzoic acid To a stirred solution of 30 mg (0.058 mmol, 1.0 eq.) of the compound resulting from Example 116A in 1 mL of methanol was added 0.1 mL (0.4 mmol. 6.5 eq.) of 4 N aqueous NaOH solution and stirring was continued for 2 hours. The reaction was quenched by the addition of 1 mL of 3 N aqueous HCI and poured into water and extracted 2x with ethyl acetate. The combined organic phases were washed with brine, dried, filtered and concentrated to give 29 mg (103%) of the title compound as a white foam. 1H NMR (300 MHz., CDCI3) δ 8.28 (t, 1H), 8.20 (t, 1H), 8.12 (s, 1H), 7.94 (s, 1H), 7.31 (m, 7H), 7.13 (m, 2H), 7.02 (m, 5H), 4.72 (bd, 2H), 4.40 (bd, 2H). MS (DCl, NH ₃ ) m/e 498 (42%); 481 (100%). Anal calcd for C29H24N2O5 ^• 0.56 H ₂ 0: C, 72.49; H, 5.03; N, 5.83. Found: C, 71.00; H, 5.35; N, 5.55.

Example 117

3-fN-Benzyl-N-4-phenoxybenzylaminocarbonvn-5-M H-tetrazolvnbenzoic acid methyl ester

Example 117A 3-fN-Benzyl-N-4-phenoxybenzylaminocarbonyl]-5-cyanobenzoic acid methyl ester To a stirred solution of 171 mg (0.33 mmol, 1.0 eq.) of the compound resulting from Example 116A in 3 mL of acetonitrile at -10 °C were sequentially added 0.14 mL (0.99 mmol, 3.0 eq.) of triethylamine and 29 μL (0.37 mmol, 1.1 eq.) of methanesulfonyl chloride. The cold bath was removed and the mixture stirred for 2 hours. The yellow mixture was partitioned between H2O and ethyl acetate. The combined organic extracts were dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (20 g) eluting

with 20% ethyl acetate in hexanes to give 123 mg (76 %) of the title compound. 1H NMR (300 MHz., CDCI ₃ ) δ 8.34 (m, 2H), 7.90 (s, 1H), 7.38 (m, 7H), 7.13 (m, 2H), 7.02 (m, 5H), 4.72 (bd, 2H), 4.36 (bd, 2H), 3.95 (s, 3H). MS (DCl, NH ₃ ) m e 494 (100%); 477 (85%); 217 (40%); 200 75%); 183 (93%).

Example 117B 3-fN-Benzyl-N-4-phenoxybenzylaminocarbonyπ-5-M H-tetrazolvπbenzoic acid methyl ester A mixture of 120 mg (0.25 mmol, 1.0 eq.) of the compound resulting from Example 117A, 98 mg (1.50 mmol, 6.0 eq.) of NaN3 and 206 mg (1.50 mmol, 6.0 eq.) of Et^N-HCI in 3 mL of DMF was heated in an oil bath at 100 °C for 3 hours. The cooled reaction mixture was partitioned between 3 N aqueous HCI and ethyl acetate. The aqueous phase was extracted with 2 additional portions of ethyl acetate, and the combined organics were washed with 3 portions of water and 1 portion of brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (20 g) eluting with 97:2:1 CHCI3- methanol-acetic acid to give 128 mg (98%) of the title compound as thick oil. A portion of this material was lyophilized for analysis. ¹ H NMR (300 MHz., CDCI3) δ 8.77 (s, 1H), 8.44 (s, 1H), 8.18 (s, 1H), 7.36 (m, 7H), 7.15 (m, 2H), 7.04 (m, 5H), 4.83 (bd, 2H), 4.51 (bd, 2H), 3.90 (2s (due to isomeric tetrazoles), 3H). MS (DCl, NH ₃ ) m/e 520 (95%); 183 (100%). Anal calcd for C30H25N5O4 • 0.61 H 0: C, 69.35; H, 4.85; N, 13.48. Found: C, 67.92; H, 4.93; N, 13.21.

Example 118 3-[N-Benzyl-N-4-phenoxybenzylaminocarbonyl]-5-f1 H-tetrazolvhbenzoic acid Using the procedures of Example 116B and the compound resulting from Example 117B, the title compound was prepared. ¹ H NMR (300 MHz., CDCI3) δ 8.65 (s, 1H), 8.44 (s, 1H), 8.21 (s, 1H), 7.32 (m, 7H), 7.09 (m, 2H), 6.98 (m, 5H), 4.80 (bd, 2H), 4.46 (bd, 2H). MS (DCl, NH ₃ ) m/e 523 (10%); 506 (100%); 415 (20%); 366 (15%). Anal calcd for C29H23N5O4 ^■ 0.75 H ₂ 0: C, 68.90; H, 4.85; N, 13.85. Found: C, 67.19; H, 4.91; N, 13.16.

Example 119 3-[N-Benzyl-N-4-phenoxybenzylaminocarbonyl]-5-(N- hvdroxyaminocarbonvI)benzoic acid To a stirred solution of 99 mg (0.2 mmol, 1.0 eq.) of the compound resulting from Example 114A in 2 mL of CH2Cl2 at 0 °C were sequentially added 46 μL (0.42 mmol, 2.10 eq.) of N-methylmorpholine and 27 μL (0.21 mmol, 1.05 eq.) of isobutylchloroformate, and the solution was stirred for 30 minutes. To the reaction mixture was added 32 mg (0.22 mmol, 1.1 eq.) of O- tert-butyldimethylsilyl-hydroxylamine-HCI and stirring was continued for 2 hours. The reaction mixture was concentrated, the residue was dissolved in 2 mL of THF, and 1 mL of 1 ϋ aqueous HCI was added. The mixture was stirred ovemight and partitioned between ethyl acetate (3x) and water. The combined organic phases were washed with brine, dried, filtered and concentrated. The crude residue was dissolved in 2 mL of 1 :1 THF methanol, 0.5 mL of 4 N aqueous NaOH solution was added, and the mixture was stirred ovemight. The reaction mixture was acidified with excess 3 K aqueous HCI and poured into a separatory funnel containing water and ethyl acetate. The aqueous phase was extracted with 2 additional portions of ethyl acetate, and the combined organic phases were washed with brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (15 g) eluting with 94:5:1 CHCl3-methanol-acetic acid to give 37 mg (37%) of the title compound as a white foam. Lyophilization gave a white powder. ¹ H NMR (300 MHz., DMSO- de) δ 8.36 (s, 1H), 8.04 (s, 1 H), 7.96 (s, 1H), 7.35 (m, 7H), 7.13 (m, 3 _. H), 6.99 (m, 4H), 4.62 (bd, 2H), 4.44 (bd, 2H). MS (DCl, NH ₃ ) m/e 497 (100%); 453 (18%); 183 (45%); 117 (22%); 111 (31%). Anal calcd for C29H24N2O6 - 1.18 H ₂ 0: C, 70.15; H, 4.87; N, 5.64. Found: C, 67.27; H, 4.90; N, 5.14.

Example 120 5-[N-Benzyl-N-4-phenoxybenzylaminocarbonylamino]benzene-1.3- dicarboxylic acid

Example 120A

5-fN-Benzyl-N-4-phenoxybenzylaminocarbonylamino1-1.3- dimethoxycarbonylbenzene To a stirred solution of the compound resulting from Example 110A (238 mg, 1.0 mmol, 1.0 eq.) in 10 mL of dry dioxane was added 0.24 mL (2.2 mmol, 2.2 eq.) of N-methylmoφhoiine followed by 0.24 mL (1.1 mmol, 1.1 eq.) of diphenylphosphoryl azide, and stirring was continued for 1 hour. The resulting solution was heated to 80 °C for 1 hour and then allowed to cool to room temperature. To the yellow/brown solution was added 358 mg (1.1 mmol, 1.1 eq.) of N-benzyl-N-(4-phenoxybenzyl)amine-HCI and the suspension stirred at room temperature for 12 hours and at reflux for 4 hours. The cooled reaction mixture was concentrated and the residue purified by column chromatography on S1O2 (25 g) eluting with 30% ethyl acetate in hexanes to give 299 mg (57%) of the title compound as a white foam. ¹ H NMR (300 MHz., CDCI3) δ 8.32 (t, 1H), 8.12 (s, 1H), 8.11 (s, 1H), 7.33 (m, 9H), 7.12 (m, 1H), 7.03 (m, 4H), 6.53 (s, 1 H), 4.62 (s, 4H), 3.90 (s, 3H). MS (DCl, NH ₃ ) m/e 542 (10%); 290 (100%); 270 (12%).

Example 120B 5-[N-Benzyl-N-4-phenoxybenzylaminocarbonylamino]benzene-1.3- dicarboxylic acid

Using the procedure described in Example 112 and the compound resulting from Example 120A, the title compound was prepared. ¹ H NMR (300 MHz., CD3OD) δ 8.32 (m, 1H), 8.30 (m, 2H), 7.31 (m, 9H), 7.09 (m, 1H), 6.96 (m, 4H), 4.64 (s, 2H), 4.60 (s, 2H). MS (FAB+) m/e 497 (62%); 405 (30%); 289 (18%); 183 (28%); 154 (100%); 136 (85%); 107 (30%). MS (FAB-): 495 (M-H, 70%); 459 (18%); 306 (30%); 199 (28%); 168 (28%); 153 (100%). HRMS: calcd for C29H25N2O6: 497.1713. Found: 497.1708.

Example 121a 5-[N-Benzyl-N-4-phenoxybenzylaminosulfonvn-3-methoxycarbonyl benzoic acid and Example 121b 5-fN-Benzyl-N-4-phenoxybenzylaminosulfonyl]benzene-1.3-dicar boxylic acid

Example 121 A 3.5-Bisfmethoxycarbonyhbenzenesulfonyl chloride To a stirred suspension of 548 mg (2 mmol, 1 eq.) of 3,5- bis(methoxycarbonyI)-benzenesulfonic acid in 10 mL of 1 ,2-dichloroethane was added 0.30 mL (4 mmol, 4 eq.) of thionyl chloride and 2 drops of DMF. The mixture was then heated to reflux for 20 hours whereupon an additional 0.30 mL (4 mmol, 4 eq.) of thionyl chloride was added. After 3 more hours at reflux, the suspension was filtered while hot and concentrated to give 311 mg (53%) of the title compound as a white solid. ¹ H NMR (300 MHz., CDCI3) δ 9.02 (m, 1H), 8.86 (m, 2H), 4.03 (s, 6H). MS (DCl, NH ₃ ) m/e 327 (40%); 310 (100%); 212 (70%).

Example 121B 3.5-Bis(methoxycarbonvπ-1-rN-benzyl-N-(4- phenoxybenzy!)1benzenesulfonamide To a stirred suspension of 358 mg (1.1 mmol, 1.1 eq.) of N-benzyl-N-(4- phenoxybenzyl)amine-HCI in 5 mL of CH2CI2 at 0 °C was added 0.42 mL 2.4 mmol, 2.4 eq.) of Hunigs base. After stirring the mixture for 5 minutes, a solution of the compound resulting from Example 121 A (292 mg, 1.0 mmol, 1.0 eq.) in 5 mL of CH2CI2 was added dropwise, the solution was stirred 20 minutes at 0 °C and ovemight at room temperature and concentrated to dryness. The residue was purified by careful column chrmoatography on Siθ2 (25 g) eluting with 25% ethyl acetate in hexanes to give 321 mg (59%) of the title compound as a white foam. ¹ H NMR (300 MHz., CDCI3) δ 8.84 (m, 1 H), 8.58 (m, 2H), 7.34 (m, 2H), 7.23 (m, 3H), 7.14 (m, 3H), 7.06 (m, 2H), 6.95 (m, 2H), 6.86 (m, 2H), 4.42 (s, 2H), 4.37 (s, 2H), 3.99 (s, 6H). MS (DCl, NH ₃ ) m/e 563 (40%); 288 (30%); 217 (47%); 200 (100%).

Example 121a 5-fN-Benzyl-N-4-phenoxybenzylaminosulfonyl -3-methoxycarbonylbenzoic acid and Example 121b

5-fN-Benzyl-N-4-phenoxybenzylaminosulfonyl]benzene-1.3-di carboxylic acid To a solution of 310 mg (0.57 mmol, 1.0 eq.) of the compound resulting from Example 121B in 7.5 mL of THF at 0 °C was added a solution of 36 mg (0.85 mmol, 1.5 eq.) of UOH-H2O in 2.5 mL of H2O. Methanol was added to obtain a homogeneous solution and the mixture was stirred for 2 hours and quenched by the addition of 3 N aqueous HCI. The quenched mixture was partitioned between ethyl acetate (2x) and 3 NJ aqueous HCI. The combined organic phases were washed with brine, dried, filtered and concentrated. The residue was purified by column chromatography on S1O2 (25 g) eluting with 98:1.5:0.5 CHCl3-methanol-acetic acid to give 151 mg (50%) the monester 121a as a white foam. ^" >H NMR (300 MHz., DMSO-d ₆ ) δ 8.62 (t, 1H), 8.41 (t, 1H), 8.34 (t, 1H), 7.38 (m, 2H), 7.23 (m, 3H), 7.15 (m, 5H), 6.88 (m, 2H), 6.82 (m, 2H), 4.44 (s, 2H), 4.39 (s, 2H), 3.93 (s, 3H). MS (FAB+) m/e 531 (70%); 183 (60%); 154 (100%); 136 (70%). MS (FAB ^* ): 530 (95%); 306 (59%); 305 (58%); 168 (40%); 153 (100%); 122 (18%). Anal calcd for C29H25NO7S: C, 65.53; H, 4.74; N, 2.63. Found: C, 65.78; H, 4.82; N, 2.60. Furth

Further elution with 94:5:1 CHC -methanol-acetic acid gave 123 mg (42%) of the diacid 121b as a white solid. 1H NMR (300 MHz., DMSO-d ₆ ) δ 8.63 (t, 1H), 8.39 (m, 2H), 7.38 (m, 2H), 7.24 (m, 3H), 7.16 (m, 5H), 6.88 (m, 2H), 6.81 (m, 2H), 4.43 (s, 2H), 4.38 (s, 2H). MS (FAB+) m/e 540 (15%); 517 (M+, 40%); 307 (20%); 183 (45%); 154 (100%); 136 (75%); 107 (25%). MS (FAB.): 516 (84%); 30650%); 305 (48%); 246 (17%); 199 (36%); 168 (39%); 153 (100%); 122 (18%). Anal calcd for C28H23 O7S: C, 64.98; H, 4.48; N, 2.71. Found: C, 74.79; H, 4.65; N, 2.52.

Example 122

4-[N-Benzyl-N-4-phenoxybenzylaminocarbonylamino]-2- carboxymethoxybenzoic acid

Example 122A

4-t-Butoxycarbonylaminosalicylic acid To a stirred suspension of 3.82 g (25 mmol, 1 eq.) of 4-aminosalicylic acid in 100 mL of 3:1 THF-H2O was added 10.36 g (75 mmol, 3 eq.) of K2CO3 in 50 mL of H2O. After stirring for an additional 10 minutes, a solution of 6.54 g (30 mmol, 1.2 eq.) of di-t-butyl-dicarbonate in 50 mL of THF was added and the biphasic mixture stirred vigorously for 72 hours. The mixture was concentrated to remove the THF, and the aqueous phase was carefully acidified with 3 JN aqueous HCI. This mixture was then extracted with 3 portions of ethyl acetate and the combined organic phases were washed with brine, dried, filtered, and concentrated. The resulting brown solid was recrystallized from toluene/ethyl acetate to give 3.54 g of the title compound as a light brown solid. ¹ H NMR (300 MHz., CDCI ₃ ) δ 10.53 (s, 1H), 8.82 (d, 1H), 7.07 (d, TH), 6.92 (dd, 1H), 6.71 (bs, 1H), 1.54 (s, 9H). MS (DCl, NH3) m/e 271 (100%); 236 (10%); 215 (20%); 136 (38%).

Example 122B 4-t-Butoxycarbonylaminosalicylic acid methyl ester To a stirred solution of 1.68 g (6.63 mmol) of the compound resulting from Example 122A in 20 mL of 10% methanol in ether at 0 °C was added excess trimethylsilyl-CH2N2. (TMS CH2N2) The yellow solution was stirred at 0 °C for 30 minutes and at room temperature for 30 minutes and the excess TMSCH2N2 was quenched by the addition of excess acetic acid. The solution was concentrated and the residue recystallized from hexanes to give 1.31 g (75%) of the title compound as a white solid. ¹ H NMR (300 MHz. CDCI3) δ 10.84 (s, 1 H), 7.74 (d, 1 H), 6.98 (d, 1 H), 6.93 (dd, 1 H), 6.57 (bs, 1 H), 3.92 (s, 3H), 1.53 (s, 9H). MS (DCl, NH ₃ ) m/e 285 (100%); 268 (18%); 229, (30%).

Example 122C 4-ft-ButoxycarbonylaminoV2-methoxycarbonylmethoxybenzoic acid methyl ester To a stirred solution of 267 mg (1.0 mmol, 1.0 eq.) of the compound resulting from Example 122B in 2 mL of DMF at room temperature were sequentially added 0.11 mL (1.2 mmol, 1.2 eq.) of methyl bromoacetate and 152 mg (1.1 mmol, 1.1 eq.) of K2CO3, and the suspension was vigorously stirred for 12 hours. The mixture was partitioned between water and ethyl acetate (2x). The combined organic phases were washed with 2 portions of water and 1 portion of brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (25 g) eluting with 30% to 50% ethyl acetate in hexanes to give the title compound as a thick oil. ¹ H NMR (300 MHz., CDCI3) δ 7.83 (d, 1H), 7.27 (d. 1H), 6.83 (dd, 1H), 6.64 (bs, 1H), 4.74 (s, 2H), 3.88 (s, 3H), 3.82 (s, 3H), 1.5,( s, 9H). MS (DCl, NH ₃ ) m/e 357 (100%); 340 (55%); 283 (15%).

Example 122D 4-Amino-2-methoxycarbonylmethoxybenzoic acid methyl ester The compound resulting from Example 122C (250 mg) was dissolved in 2 mL of 4 N HCL in dioxane and stirred for 8 hours. The resulting solution was concentrated to dryness to give 177 mg (100%) of the title compound as a thick oil. 1H NMR (300 MHz., CDCI3) δ 7.74 (bd, 1H), 7.09 (m, 2H), 4.72 (s, 2H), 3.88 (s, 3H), 3.71 (s, 3H). MS (DCl, NH3) m e 257 (100%); 240 (80%).

Example 122E

N-Benzyl-N-(4-phenoxybenzvπ-carbamoyl chloride To a suspension of 3.26 g (10 mmol, 1.0 eq.) of N-benzyI-N-(4- phenoxybenzyl)-amine-HCI in 50 mL of toluene was added 100 mL of 4 t± aqueous NaOH, and the mixture was vigorously stirred until all of the solid dissolved (-30 minutes). To the resulting clear, biphasic mixture was added 7.8 mL of a 1.93 M solutuon of phosgene in toluene (15 mmol, 1.5 eq.) dropwise. A precipitate formed immediately, and the mixture was vigorously stirred until all of the solid had dissolved (~ 30 minutes). The layers were

separated, and the organic phase was dried, filtered and concentrated to give an oil that solidified on standing (3.42 g, 97%). This material was used without further purification. 1H NMR (300 MHz., CDCI ₃ ) δ 7.47 (m, 4H), 7.25 (m, 6H), 7.13 (m, 1H), 7.02 (m, 3H), 4.63 (d, 2H), 4.52 (d, 2H). MS (DCl, NH ₃ ) m/e 370, 351, 290, 224, 217, 200, 183.

Example 122F

4-[N-Benzyl-N-4-phenoxybenzylaminocarbonylamino]-2- methoxycarbonylmethoxybenzoic acid methyl ester To a stirred solution of 175 mg (0.63 mmol, 1.0 eq.) of the compound resulting from Example 122D in 2 mL of pyridine was added sequentially, 19 mg (0.16 mmol, 0.25 eq.) of DMAP and 268 mg (0.76 mmol, 1.2 eq.) of the compound resulting from Example 122E, and the mixture was stirred at room temperature for 5 hours, at 55 °C for 16 hours and 24 hours at 100 ^C C. The cooled reaction mixture was poured into 3 N aqueous HCI and extracted with 3 portions of ethyl ether. The combined organic extracts were washed 3x with 3 aqueous HCI and once with brine, dried, filtered, and concentrated. The residue was purified by column chromatography on Siθ2 (20 g) eluting with 40% ethyl acetate in hexanes to give 25 mg (7%) of the title compound as an oil. 1H NMR (300 MHz., CDCI3) δ 7.76 (d, 1H), 7.24 - 7.43 (m, 10H), 7.12 (m, 1H), 7.01 (m, 4H), 6.52 (m, 2H), 4.73 (s, 2H), 4.60 (s, 4H), 3.87 (s, 3H), 3.81 (s, 3H). MS (DCl, NH3) m/e 572 (40%); 555 (18%); 290 (100%); 283 (78%).

Example 122G 4-[N-Benzyl-N-4-phenoxybenzylaminocarbonylamino]-2- carboxymethoxybenzoic acid Using the procedure described in Example 112 and the compound resulting from Example 122F, the title compound was prepared as a lyophilate. 1H NMR (300 MHz., CDCI3) δ 7.92 (d, 1H), 7.24 - 7.43 (m, 10H), 7.12 (m, 1H), 7.01 (m, 4H), 6.52 (m, 1 H), 4.82 (s, 2H), 4.63 (s, 4H). MS (FAB+) m/e 527 (45%); 509 (39%); 183 (20%); 154 (100%); 136 (80%). Anal calcd for C30H26N2O7 • 1.26 H ₂ 0: C, 68.43; H, 4.98; N, 5.32. Found: C, 65.69; H, 5.44; N, 4.53.

Example 123 5-rN-Benzyl-N-4-phenoxybenzylaminocarbonylamino>2- carboxymethoxybenzoic acid The title compound was prepared starting with 5-aminosalicylic acid and using the procedures described in Example 122. ¹ H NMR (300 MHz., CDCI3) δ 7.62 (m, 2H), 7.21 - 7.46 (m, 8H), 7.11 (m, 1H), 7.02 (m, 5H), 6.88 (m, 1H), 6.40 (bs, 1H), 4.74 (bs, 2H), 4.61 (m, 4H). MS (FAB+) m/e 527 (37%); 307 (22%); 183 (18%); 155 (26%); 154 (100%); 136 (67%). Anal calcd for C30H26N2O7 • 0.23 H ₂ 0: C, 68.43; H, 4.98; N, 5.32. Found: C, 67.91; H, 5.27; N, 5.33.

Example 124 4-f 1 -(4-Phenoxyphenvπ-2-phenylethoxy]benzene-1.2-dicarboxylic acid

Example 124A 1-(4-Phenoxyphenv0-2-phenylethanol

To a solution of 595 mg (3 mmol, 1 eq.) of 4-phenoxybenzaldehyde in 6 mL of THF at -10 °C was dropwise added 2.25 mL of a 2.0 M solution of benzylmagnesium chloride (4.5 mmol, 1.5 eq.) in THF. The solution was stirred at -10 °C for 1 hour and quenched by the careful addition of saturated aqueous NH4CI. The cloudy mixture was partitioned between dilute aqueous HCI and ethyl acetate. The organic phase was washed with brine, dried, filtered and concentrated. The residue was purified by column chromatography on Siθ2 (25 g) eluting with 10% ethyl acetate in hexanes to give 580 mg (67%) of the title compound as an oil. ^" >H NMR (300 MHz., CDCI3) δ 7.18 - 7.40 (m, 10H), 7.11 (m, 1 H), 7.00 (m, 3H), 4.90 (m, 1 H), 3.03 (dd, 1H), 3.01 (dd, 1H), 1.94 (bs,.1H). MS (DCl, NH3) m/e 308 (12%); 290 (MH+, 63%); 273 (100%).

Example 124B 4-ri-(4-Phenoxyphenvπ-2-phenylethoxy|benzene-1.2-dicarboxyl ic acid dimethyl ester

To a stirred solution of 145 mg (0.5 mmol, 1.0 eq.) of the alcohol resulting from Example 124A in 8 mL of CH2CI2 at 0 °C were sequentially added 210 mg (1.0 mmol, 2.0 eq.) of 4-hydroxydimethylphthalate and 328 mg (1.25 mmol, 2.5

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eq.) of triphenylphosphine. To this solution was added a solution of 0.20 mL (1.25 mmol, 2.5 eq.) of diethyl azodicarboxylate in 2 mL of CH2CI2 dropwise. The yellow solution was stirred for 1 hour at 0 °C and for 30 minutes at room temperature. The reaction mixture was concentrated to dryness and the residue purified by column chrmoatography on Siθ (20 g) eluting with 15% ethyl acetate in hexanes to give 130 mg (54%) of the title compound as a thick oil. 1H NMR (300 MHz., CDCI3) δ 7.67 (d, 1H), 7.34 (m, 2H), 7.07 - 7.30 (m, 8H), 7.01 (m, 3H), 6.93 (d, 2H), 6.86 (dd, 1H), 5.35 (m, 1H), 3.88 (s, 3H), 3.83 (s, 3H), 3.32 (dd, 1H), 3.11 (dd, 1H). MS (DCl, NH3) m/e 500 (50%); 483 (100%); 292, (77%); 273 (63%); 239 (28%).

Example 124C 4-r 1 -f4-Phenoxyphenvπ-2-phenylethoxy]benzene-1 ,2-dicarboxylic acid The title compound was prepared using the procedures described in Example 112 and the compound resulting from Example 124B. ¹ H NMR (300 MHz., CDCI3) δ 7.90 (m, 1H), 7.34 (m, 2H), 7.07 - 7.38 (m, 8H), 6.85 - 7.06 (m, 6H), 5.38 (m, 1H), 3.36 (bdd, 1H), 3.11 (bdd, 1H). MS (FAB+) m/e 493 (10%; 477 (23%); 455 (30%); 273 (100%); 180 (58%); 154 (80%); 136 (66%). Anal cald for C28H22O6 ^■ 0.63 EtOAc: C, 73.99; H, 4.88. Found: C, 71.89; H, 5.51.

Example 125 2-Allyloxycarbonyl-4.5-dicarboxylic anhvdride-N-benzyl-n-f4- phenoxybenzvObenzamide Under N2, a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.242 g, 1.00 mmol) in anhydrous THF (10 mL) was cooled to 0 °C and subsequently treated in a dropwise manner with a solution of N-benzyl-N-(4- phenoxybenzyl)amine (0.289 g, 1.00 mmol) and DBU (0.171 g, 1.10 mmol) in anhydrous THF (10 mL). After 30 minutes allyl iodide (0.514 g, 3.00 mmol) was added, and the reaction mixture was allowed to warm to ambient temperature. After 14 hours the reaction mixture was concentrated under reduced pressure, and the residue was dissolved in EtOAc (4 mL). The solution was extracted with 1 N aqueous HCI (2 x 3 mL), dried (Na2Sθ4), and then concentrated under reduced pressure to provide a tan foam. Flash column chromatography eluting

with 80:20 to 70:30 hexane: EtOAc afforded compound the title compound (0.065 g, 11% yield). 1H NMR (300 MHz, CDCI3) δ 4.20 (d, 2 H), 4.60-4.75 (m, 4 H), 5.22-5.42 (m, 2 H), 5.83-6.04 (m, 1 H), 6.85-7.40 (m, 15 H), 8.02 (m, 1 H). LRMS (DCl): 548 (M+H)+.

Example 126a 2-Allyloxycarbonyl-4.5-dicarboxyl-N-benzyl-N-f4-phenoxybenzv n-benzamide and Example 126b 2-Allyloxycarbonyl-4-benzyloxycarbonyl-5-carboxyl-N-benzyl-N -(4- phenoxybenzvO-benzamide and 2-Allyloxycarbonyl-5-benzyloxycarbonyl-4-carboxyl-N-benzyl-N -f4- phenoxybenzvfl-benzamide Under a N2 atmosphere, a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.242 g, 1.00 mmol) in anhydrous MeCN (10 mL) was cooled to 0 °C and subsequently treated in a dropwise manner with a solution of N- benzyl-N-(4-phenoxybenzyl)amine (0.289 g, 1.00 mmol) and DBU (0.171 g, 1.10 mmol) in anhydrous MeCN (10 mL). After 30 minutes, allyl iodide (0.514 g, 3.00 mmol) was added, and the reaction mixture was heated to reflux for 4 hours. After cooling to ambient temperature, the reaction mixture was treated with benzyl alcohol (0.437 g, 4.00 mmol) and returned to reflux. After 14 hours the reaction mixture was concentrated under reduced pressure, and the residue was dissolved in EtOAc (4 mL). The solution was extracted with 1 t± aqueous HCI (5 x 2 mL), dried (Na2Sθ4) and concentrated under reduced pressure to provide a dark oil. Flash column chromatography on acidic silica gel, prepared by stirring in 2% H3PO4 in MeOH for 5 hours followed by filtration, eluting with 70:30 going to 60:40 hexane-EtOAc afforded, after lyophilization from CH3CN-H2O, a mixture of the title compounds 126b as a white powder (0.322 g, 49% yield). 1H NMR (300 MHz, CDCI3) δ 4.11-4.23 (m, 2H), 4.62-4.82 (m, 4H), 5.25-5.43 (m, 4H), 5.88-6.04 (m, 1H), 6.84-7.18 (m, 7H), 7.20-7.41 (m, 12H), 7.63 (m, 1H of one isomer), 7.82 (m, 1H of second isomer), 8.40 (m, 1H of second isomer), 8.52 (m, 1H of first isomer). LRMS (DCl) m/e 656 (M+H)+. Further elution provided compound 126a (0.021 g, 4% yield). ¹ H NMR (300

MHz, CDCI3) δ 4.18 (d, 2H), 4.62-4.82 (comp, 4H), 5.23-5.42 (comp, 2H), 5.95- 6.03 (m, 1H), 6.95-7.40 (comp, 14H), 7.70 (s, 1H), 8.50 (s, 1H), 10.58 (brs, 2H). LRMS (DCl) m/e 566 (M+H)+

Example 127

2-Allyloxycarbonyl-4.5-dibenzyloxycarbonyl-N-benzyl-N-r4- phenoxyhen7yl)- benzamide Under a N atmosphere, a solution of the compounds resulting from Example 125 and 126a (0.141 g, 0.215 mmol), DBU (39.3 mg, 0.258 mmol), and benzyl bromide (45.0 mg, 0.258 mmol) in anhydrous MeCN (0.4 mL) was heated to reflux for 22 hours. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in 1:1 Et2θ-EtOAc (4 mL). The solution was washed with saturated aqueous NaHCθ3 (4 x 2 mL) and 1 N aqueous HCI (5 x 2 mL), dried (MgS04), and concentrated under reduced pressure to provide an oil. Radial chromatography eluting with 80:20 hexane- EtOAc afforded the title compound as a clear, viscous oil (94.0 mg, 59% yield). 1H NMR (300 MHz, CDCI3) δ 4.07-4.22 (m, 2H), 4.61-4.80 (m, 4H), 5.18-5.43 (m, 6H), 5.85-5.99 (m, 1H), 6.88-7.40 (m, 24H), 7.70 (m, 1H), 8.41 (m, 1H). LRMS (DCl) m/e 746 (M+H)+.

Example 128

3.4-Dibenzyloxycarbonyl-6- ^r N-benzyl-N- ⁽ 4-phenoxybenzvπaminocarbonyl]- benzoic acid Under a N2 atmosphere, Pd(PPh3)4 (9.34 mg, 0.008 mmol, 10 mol %) was added to a solution of the compound resulting from Example 127 (60.0 mg, 0.08 mmol) and morpholine (70.4 mg, 0.8 mmol) in THF (0.80 mL). After 1 hour the reaction mixture was concentrated under reduced pressure. The residue was dissolved in dichloromethane (4 mL), and the solution was washed with 2 aqueous HCI (4 x 2 mL), dried (Na2Sθ4) and concentrated under reduced pressure to provide an oil. Flash column chromatography on acidic silica gel, prepared by stirring in 2% H ₃ PO4 in MeOH for 5 hours followed by filtration, eluting with 70:30 hexane-EtOAc afforded, after lyopholization from CH3CN-H2O, the title compound as a white powder (32.3

mg, 57% yield). 1H NMR (300 MHz, CDCI ₃ ) δ 4.15 (d, J = 16.3Hz, 2H), 4.52- 4.90 (br, 1H), 5.19-5.25 (m, 4H), 6.83-7.14 (m, 6H), 7.21-7.40 (m, 19H), 7.71 (m, 1H), 8.43 (m, 1H). LRMS (DCl) m/e 706 (M+H)+.

Example 129

3-Benzyloxycarbonyl-6-[N-benzyl-N-f4-phenoxybenzvπaminoc arbonvn-1.4- dibenzoic acid and 4-Benzyloxycarbonyl-6-fN-benzyl-N-f4-phenoxybenzyπaminocarb onyl]-1.3- dibenzoic acid Under a N2 atmosphere, a solution of benzyl alcohol (0.109 g, 1.00 mmol) and triethylamine (0.102 g, 1.00 mmol) in THF (5 mL) was added dropwise to a solution of 4,5-dichlorocarbonylphthalic anhydride (0.136 g, 0.500 mmol) in THF (5 mL) at -30 °C. After 30 minutes, a solution of N-benzyl- N-(4-phenoxybenzyl)amine (0.145 g, 0.500 mmol) and EtβN (51.5 mg, 0.500 mmol) in THF (2.5 mL) was added, and the reaction mixture was allowed to warm to ambient temperature. After 45 minutes, the reaction mixture was concentrated under reduced pressure, and the residue was dissolved in EtOAc (5 mL). The solution was washed with 1 M aqueous HCI (2 x 2 mL), dried (Na2S04) and concentrated under reduced pressure to provide crude product as a white foam. Flash column chromatography on acidic silica gel, prepared by stirring in 2% H3PO4 in MeOH for 5 hours followed by filtration, eluting with 70:30 going to 60:40 hexane-EtOAc afforded a mixture of the title compounds as a white powder (0.109 g, 35% yield). 1H NMR (300 MHz, CDCI3) δ 4.15- 4.25 (m, 2H), 4.25-5.25 (br, 2H), 5.28-5.30 (m, 2H), 6.82-7.10 (comp, 9H), 7.20- 7.40 (comp, 15H), 7.64-7.80 (m, 1H), 8.25-8.32 (m, 1H), 10.05 (br, 2H). LRMS (DCI): 616 (M+H)+.

Example 130 5-fN-f2-Ethoxybenzvπ-N-(4-phenoxybenzvπaminocarbonvnbenzen e-1.2.4- tricarboxylic acid

N-2-Ethoxybenzyl-N-(4-phenoxybenzyl) amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl) amine substituting 2- ethoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2-ethoxybenzyl)-N-(4-phenoxybenzyl)-amine in place of N- benzyl-N-(4-phenoxybenzyl)amine. 1H NMR (DMSO-dβ, 300 MHz) δ 1.2 (dt, 3H), 3.90 (dq, 2H), 4.20 (d, 2H), 4.60 (s, 2H), 6.80 - 7.25 (m, 13H), 7.90 (s, 1H), 8.45 (s, 1 H). MS (FAB)+ m/e 570 (M+H)+ and (FAB)" m/e 568 (M-H) ^* .

Example 131 5-fN-f3-Methylbenzvπ-N-^4-ρhenoxybenzvπaminocarbonynbenze ne-1.2.4- tricarboxylic acid N-3-Methylbenzyl-N-(4-phenoxybenzyl)amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl)-amine substituting 3- methylbenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(3- methyIbenzyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.8 (s, 3H), 4.20 (d, 2H), 4.75 (s, 2H), 6.70 -7.60 (m, 13H), 7.80 (d, 1H), 8.45, (d, 1H). MS (FAB)+ m/e 556 (M+H)+ and (FAB)" m/e 554 (M-H)-.

Example 132 5-fN-f2.3-Dimethoxybenzvπ-N-f4-phenoxybenzvπaminocarbonyl] benzene-

1.2.4-tricarboxylic acid N-2,3-Dimethoxybenzyl-N-(4-phenoxybenzyl)amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2,3-dimethoxybenzyl amine for benzyl amine. The title compound was prepared by the procedures described in

Example 4 using N-(2,3-dimethoxybenzyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.70 (s, 3H), 3.80 (s, 3H), 4.20 (d, 2H), 4.70 (s, 2H), 6.80 - 7.45 (m, 12H), 7.60 (s, 1 H), 8.25 (s, 1 H). MS (FAB)+ m/e 586 (M+H)+ and (FAB)- m/e 584 (M-H)-.

Example 133 5-fN-f4-Methoxybenzvπ-N-f4-phenoxybenzvπaminocarbonyl1benz ene-1.2.4- tricarboxylic acid N-4-Methoxybenzyl-N-(4-phenoxybenzyl)amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl)-amine substituting 4- methoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(4- methoxybenzyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.75 (s, 3H), 4.20 (s, 2H), 4.60 (b, 2H), 6.80 - 7.45 (m, 13H), 7.70 (d, 1H), 8.35 (s, 1H). MS (FAB)+ m/e 556 (M+H)+ and (FAB)" m/e 554 (M-H)-.

Example 134 5-fN-(2.6-Dimethoxybenzy0rN-(4-phenoxybenzyπaminocarbonyl]b enzene- 1.2.4-tricarboxylic acid

N-2,6-Dimethoxybenzyl-N-(4-phenoxybenzyl)amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2,6-dimethoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4using N-(2,6-dimethoxybenzyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.65 (s, 6H), 4.15 (d, 2H), 4.50 (s, 2H), 6.40 - 7.45 (m, 12H), 7.90 (s, 1H), 8.45 (s, 1H). MS (FAB)+ m/e 586 (M+H)+.

Example 135

5-rN-^2-n-Hexoxybenzvπ-N-^4-phenoxybenzvπaminocarbonyl1 benzene-1.2.4- tricarboxylic acid N-2-n-Hexoxybenzyl-N-(4-phenoxybenzyl) amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl)-amine substituting 2-n-hexoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- n-hexoxybenzyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 0.90

(m, 3H), 1.20 - 1.60 (m, 6H), 3.80 - 4.00 (m, 2H), 4.20 (d, 2H), 4.70 (s, 2H), 6.80 - 7.25 (m, 13H), 7.90 (s, 1H), 8.45 (s, 1H). MS (FAB)+ m/e 626 (M+H)+ and (FAB)" m/e 624 (M-H)-.

Example 136

5-fN-f2-Phenoxybenzvn-N-f4-Dhenoxybenzvπam?nocarbonvπbe nzene-1.2.4- tricarboxylic acid N-2-Phenoxybenzyl-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)-amine substituting 2- phenoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- phenoxybenzyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 4.20 (d, 2H), 4.65 (s, 2H), 6.60 - 7.85 (m, 18H), 7.95 (d, 1H), 8.50 (d, 1H). MS (FAB)+ m/e 618 (M+H)+ and (FAB)" m/e 616 (M-H) ^* .

Example 137 5-fN-(2-Phenoxyethyl N-f4-phenoxybenzyflaminocaιftonvπbenzene-1.2.4- tricarboxylic acid N-2-Phenoxyethyl-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyI)-amine substituting 2- phenoxyethyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2-phenoxyethyl)-N-(4-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.20 -

3.40 (m, 2H), 4.0 (m, 2H), 4.20 (s, 2H), 6.80 - 7.45 (m, 13H), 7.85 (s, 1H), 8.25 (s, 1 H). MS (FAB)+ m/e 618 (M+H)+ and (FAB)- m/e 616 (M-H)-.

Example 138 5-fN-(2-Methoxyphenethvn-N-f4-phenoxybenzyl)aminocarbonvπbe nzene-1.2.4- tricarboxylic acid N-2-Methoxyphenethyl-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-methoxyphenethyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- methoxyphenethyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 2.60 - 3.00 (m, 4H), 3.40 (s, 3H), 4.15 (s, 2H), 6.70 - 7.45 (m, 13H), 7.45 (s, 1H), 8.25 (s, 1 H). MS (FAB)+ m/e 570 (M+H)+ and (FAB)" m/e 568 (M-H)-.

Example 139 5-[N-f2-Ethoxybenzvπ-N-f3-phenoxybenzvπaminocarbonvnbenzen e-1.2.4- tricarboxylic acid

N-2-Ethoxybenzyl-N-(3-phenoxybenzyl)amine was prepared using the procedure described for N-benzyl-N-(4-phenoxybenzyl)-amine substituting 2- ethoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- ethoxybenzyl)-N-(3-phenoxybenzyl)-amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 1.20 (m, 3H), 3.80 -4.00 (m, 2H), 4.20 (d, 2H), 4.60 (s, 2H), 6.80 - 7.50 (m, 13H), 7.55 (d, 1H), 8.25 (d, 1H). MS (FAB)+ m/e 570 (M+H)+ and (FAB)' m/e 5.68 (M-H)-.

Example 140

5-fN-M.4-Benzodioxan-2-ylmethyl)-N-(4- phenoxybenzvDaminocarbonvπbenzene-l .2.4-tricarboxylic acid N-(1 ,4-Benzodioxan-2-ylmethyl)-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4- phenoxybenzyl)amine substituting 1 ,4-benzodioxan-2-ylmethyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(1 ,4-benzodioxan-2-ylmethyl)-N-(4-phenoxybenzyl)amine

in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-dβ, 300 MHz) δ 4.10 (m, 1H), 4.20 (t, 2H), 4.70 (s, 2H), 6.80 - 7.35 (m, 13H), 7.40 (m, 1H), 7.70 (m, 1H). MS (FAB)" m/e 580 (M-H)-.

Example 141

5-fN-f3-Phenoxypropyn-N-f4-phenoxybenzvhaminocarbonvn benzene-1.2.4- tricarboxylic acid N-3-Phenoxypropyl-N-(4-phenoxybenzyl) amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 3- phenoxypropyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(3- phenoxypropyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. 1H NMR (DMSO-d ₆ , 300 MHz) δ 2.10 -3.10 (m, 6H), 4.15 (m, 2H), 6.80 - 7.45 (m, 14H), 7.85 (m, 1H), 8.25 (m, 1H). MS (FAB)+ m/e 570 (M+H)+ and (FAB)" m/e 568 (M-H)-.

Example 142 5-fN-f2-(2-Ethoxyphenoxy)ethvn-N-f4-phenoxybenzvnaminocarbon yl]ben7ene-

1.2.4-tricarboxylic acid N-2-(2-Ethoxyphenoxy)ethyl-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-(2-ethoxyphenoxy)ethylamine for benzylamine.

The title compound was prepared by the procedures described in Example 4 using N-(2-(2-ethoxyphenoxy)ethyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 1.25 (m,3H), 4.00-4.20 (m, 6H), 4.50 (s, 2H), 6.80 - 7.65 (m, 13H), 8.10 (s, 1H), 8.80 (d, 1H). MS (FAB)" m/e 598 (M-H) ^" .

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Example 143 5-rN-f2-Phenoxyethvπ-N-^3-f4-methylphenoxy)benzvπaminocarb onvnbenzene-

1.2.4-tricarboxylic acid N-(2-Phenoxyethyl)-N-(3-(4-methylphenoxy)benzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-phenoxyethylamine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2- phenoxyethyl)-N-(3-(4-methylphenoxy)benzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-dβ, 300 MHz) δ 1.95 (s, 3H), 3.95 - 4.20 (m, 2H), 4.40 (m, 2H), 6.80 - 7.30 (m, 13H), 7.95 (s, 1H), 8.40 (s, 1H). MS (FAB)+ m/e 570 (M+H) ⁺ and (FAB)" m/e 568 (M-H)".

Example 144 5-fN-f3-f2-Methoxyphenyl)propyπ-N-f4- phenoxybenzvπaminocarbonyl]benzene-1.2.4-tricarboxylic acid

N-(3-(2-Methoxyphenyl)propyl)-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4- phenoxybenzyl)amine substituting 3-(2-methoxypheny!)propylamine for benzyl amine. The title compound was prepared by the procedures described in

Example 4 using N-(3-(2-methoxyphenyl)propyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-dβ, 300 MHz) δ 2.20 - 3.00 (m, 6H), 3.60 (s, 3H), 3.80 (s, 2H), 6.60 - 7.45 (m, 13H), 7.75 (m, 1H), 8.20 (m, 1H). MS (FAB)" m/e 582 (M-H)-.

Example 145 5-rN-f3-Phenyl-2-propenvπ-N-f4-phenoxybenzyπaminocarbonyπ benzene-1.2.4- tricarboxylic acid N-(3-Phenyl-2-propenyl)-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 3-phenyl-2-propenyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(3-phenyl-2-propenyl)-N-(4-phenoxybenzyl)amine in place

of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.35 (m, 2H), 3.60 (t, H), 3.80 (d, H), 4.25 (d, 2H), 6.80 - 7.25 (m, 13H), 7.90 (s, 1H), 8.45 (s, 1H). MS (FAB)" m/e 550 (M-H)-.

Example 146

5-fN-Benzyl-N-f2-phenoxyethvπaminocarbonvπbenzene-1.2.4 -tricarboxylic acid N- Benzyl-N-(2-phenoxyethyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-phenoxyethyl amine for 4-phenoxybenzyl amine. The title compound was prepared by the procedures described in

Example 4 using N- benzyl-N-(2-phenoxyethyl)amine in place of N-benzyl-N- (4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.20 (m, 2H), 4.20 (t, 2H), 4.40 (s, 2H), 6.80 - 7.35 (m, 10H), 7.80 (s, 1H), 8.30 (s, 1H). MS (FAB)+ m/e 464 (M+H)+ and (FAB)" m/e 462 (M-H)-.

Example 147 4-rN-f2-Methoxybenzvπ-N-f4-phenoxybenzvπaminocarbonvπbenz ene-1.2- dicarboxylic acid N-(2-Methoxybenzyl)-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-methoxybenzyl amine for benzyl amine.

The title compound was prepared by the procedures described in Example 4 using N-(2-methoxybenzyl)-N-(4-phenoxybenzyl)amine in place of N-benzyl-N-(4-phenoxybenzyl)amine. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 3.70 (s, 3H), 4.40 (m, 2H), 4.60 (m, 2H), 6.80 - 7.45 (m, 14H), 7.65 (m, 1H), 7.80 (m, 1 H). MS (FAB)+ m/e 512 (M+H)+ and (FAB)- m/e 510 (M-H)-.

Example 148 5-fN-f2-Ethoxybenzvh-N-(;3-f4-methylphenoxy)benzvnaminocarbo nyllbenzene-

1 ,2.4-tricarboxylic acid N-(2-Ethoxybenzyl)-N-[3-(4-methylphenoxy)benzyl]amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-ethoxybenzyl amine for benzyl amine and 3-(4- methylphenoxy)benzaldehyde for 4-phenoxybenzaldehyde.

To a solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.43 g, 0.002 mol) in 50 mL of tetrahydrofuran cooled in a salt - ice bath was added N- methylmoφholine (0.21 g, 0.002 mol). To this mixture was added a solution of N-(2-ethoxybenzyl)-N-[3-(4-methylphenoxy)benzyl]amine (0.69 g, 0.002 mol) contained in 10 mL of tetrahydrofuran dropwise over 3 hours yja syringe pump. After stirring an additional hour, the reaction mixture was evaporated under reduced pressure at room temperature. EtOAc was added and the resulting solution washed with 5% HCI followed by a saturated solution of NaCI and concentrated under reduced pressure. A saturated solution (40 mL) of Na2Cθ2 and tetrahydrofuran (10 mL) were added and the reaction mixture stirred at room temperature for 12 hours. The tetrahydrofuran was evaporated; the basic solution acidified and extracted with ethyl acetate. The crude residue was then purified by flash silica gel chromatography eluting with with 95:4:1 CHCI3- MeOH-HOAc to afford 0.98 g (83.9%) of the title compound. 1H NMR (DMSO- de, 300 MHz) δ 1.20 (dt, 3H), 1.90 (s, 3H), 3.90 (dq, 2H), 4.20 (d, 2H), 4.65 (s, 2H), 6.80-7.50 (m, 12H), 7.55 (s, 1H), 8.25 (d, 1H). MS (FAB)+ m/e 584 (M+H)+ and (FAB)- m/e 582 (M-H)-.

Example 149 5-{N-f2-Methoxybenzvπ-N-f3-(4-methylphenoxy^benzyl1 aminocarbonyllbenzene-1.2.4-tricarboxylic acid N-2-Methoxybenzyl-N-[3-(4-methylphenoxy)benzyl]amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-methoxybenzyl amine for benzyl amine and 3-(4- methylphenoxy)benzaldehyde for 4-phenoxybenzalde.

The title compound was prepared by the procedures described in Example 148 using N-(2-methoxybenzyl)-N-[3-(4-phenoxy)benzyl]amine in place of N-2-ethoxybenzyl-N-[3-(4-methylphenoxy)benzyl]amine. ¹ H NMR (DMSO-de, 300 MHz) δ 1.90 (s, 3H), 3.60 (m, 3H), 4.20 (d, 2H), 4.60 (bs, 2H), 6.80 - 7.45 (m, 12H), 7.70 (s, 1H), 8.35 (s, 1H). MS (FAB)+ m/e 570 (M+H)+ and (FAB)" m/e 568 (M-H)-.

Example 150

5-{N-Benzyl-N-(3-chloro-4-phenoxybenzy0aminocarbonyl}benz ene-1.2.4- tricarboxylic acid N-Benzyl-N-[4-phenoxy-(3-chlorobenzyl)] amine was prepared using the same procedure as given for the preparation for N-Benzyl-N-(4- Phenoxybenzyl) amine except replacing the 4-phenoxybenzaldehyde with 4- phenoxy-3-chlorobenzaldehyde.

The title compound was prepared by the procedures described in Example 148 using N-benzyl-N-(3-chloro-4-phenoxybenzyl)amine in place of N-2-ethoxybenzyl-N-[3-(4-methylphenoxy)benzyl] amine. ¹ H NMR (DMSO-d _β , 300 MHz) δ 4.20 (d, 2H), 4.60 (b, 2H), 6.80 - 7.45 (m, 13H), 7.70 (d, 1 H), 8.30 (s, 1H). MS (FAB)+ m/e 560 (M+H)+ and (FAB)" m/e 558 (M-H)-.

Example 151 5-(N-f2-f3-Methylbutoxy)benzvπ-N-(4-phenoxybenzyπaminocarb onyl}benzene-

1 ,2.4-tricarboxylic acid N-(2-(3-MethyIbutoxy)benzyl)-N-(4-phenoxybenzyl)amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting N-(2-(3-methylbutoxy)benzyl)amine for benzyl amine.

The title compound was prepared by the procedures described in Example 148 using N-(2-(3-methylbutoxy)benzyl)-N-(4-phenoxybenzyl)amine in place of N-(2-ethoxybenzyl)-N-[3-(4-methylphenoxy)benzyl]amine. ¹ H NMR (DMSO-de, 300 MHz) δ 0.85 (dd, 6H), 3.80 - 4.00 (m, 5H), 4.20 (bd, 2H), 4.80 (bs, 2H), 6.80 - 7.45 (m, 13H), 7.70 (bs, 1 H), 8.30 (bd, 1 H). MS (FAB)+ m/e 612 (M+H)+ and (FAB)" m/e 610 (M-H)"

Example 152 5-(N-^2-Ethoxybenzvn-N-[3-f4-chlorophenoxy)benzynaminocarbon vnbenzene-

1.2.4-tricarboxylic acid N-2-Ethoxybenzyl-N-[3-(4-chlorophenoxy)benzyl]amine was prepared using the procedures described for N-benzyl-N-(4-phenoxybenzyl)amine substituting 2-ethoxybenzyl amine for benzyl amine and 3-(4- chlorophenoxy)benzaldehyde for 4-phenoxybenzaldehyde.

The title compound was prepared by the procedures described in Example 148 using N-(2-ethoxybenzyl)-N-[3-(4-chlorophenoxy)benzyI]amine in place of N-2-ethoxybenzyl-N-[3-(4-methylphenoxy)benzyl]amine . ¹ H NMR (DMSO-de, 300 MHz), δ 1.20 (dt, 3H), 3.90 (dq, 2H), 4.20 (d, 2H), 4.65 (s,2H), 6.80 - 7.45 (m, 12H), 7.70 (m, 1 H), 8.35 (m, 1 H). MS (FAB)+ m/e 604 (M+H)+ and (FAB)" m/e 602 (M-H)-.

Example 153 4-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonylmethynbiDhenyl-3 .3'.4'- tricarboxylic acid and 3-[N-Benzyl-N-f4-phenoxybenzvπaminocarbonylmethyl]biphenyl- 4.3'.4'- tricarboxylic acid

The title compound mixture was prepared by the procedures described in Example 148 using N-benzyl-N-(4-phenoxybenzyl)amine in place of N-2- ethoxybenzyl-N-[3-(4-methylphenoxy)benzyl]amine and replacing the benzene dianhydride with 4,4'-biphthalic anhydride. 1H NMR (DMSO-d ₆ , 300 MHz) δ 3.90 (d, 2H), 4.60 (b, 2H), 6.80 - 8.20 (m, 20H). MS (FAB)+ m/e 602 (M+H)+ and (FAB)" m/e 600 (M-H)-.

Example 154 4-[N-Benzyl-N-^4-phenylbutynaminocarbonylmethvnbiphenyl-1.2- dicarboxylic acid

Example 154A N- Benzyl-N-4-phenylbutylamine To a solution of benzaldehyde (3.36 g, 31.2 mmol) and 4- phenylbutylamine (4.50 g, 30.1 mmol) in 60 mL of toluene was added a catalytic amount of p-toluenesulfonic acid. The reaction flask was fitted with a Dean Stark trap and heated to reflux for 16 hours. The solvent was then evaporated, and the reaction was taken up in 100 mL EtOH. Sodium borohydride (1.14 g, 30.1 mmol) was added, and the reaction was again heated to reflux until judged complete by TLC. The reaction mixture was evaporated, taken up in EtOAc and washed with 1 N aqueous NaOH, 1 N aqueous HCI, and then saturated aqueous NaCI solution, dried over sodium sulfate, filtered and evaporated to give 7.08 g (98%) of an oil and which was used without further purification. ¹ H NMR (CDCI ₃ , 300 MHz) δ 1.46 (bs, 1H), 1.50 - 1.71 (m, 4H), 2.58 - 2.68 (m, 4H), 3.77 (s, 2H), 7.13 - 7.38 (m, 10H). MS (DCI-NH3) m/e 240 (M+H) ⁺ .

Example 154B 4-fN-Benzyl-N-f4-phenylbutynaminocarbonylmethyl1biphenyl-1.2 -dicarboxylic acid A solution of trimellitic anhydride chloride (226 mg, 1.08 mmol), triethylamine (113mg, 1.12 mmol) and N-benzyl-N-(4-phenylbutyI)amine (365 mg, 1.12 mmol) in 10 mL of CH2CI2 was stirred for 12 hours and then evaporated. The residue obtained was taken up in methanol and 100 mg of KOH was added. After 40 minutes, the reaction mixture was evaporated, 1 JbJ HCI was added, and the reaction was titrated to pH = 3 and extracted 3 times with EtOAc. The organic layers were dried over sodium sulfate, filtered and evaporated to give an oil which was purified by silica gel chromatography eluting with 5% MeOH in CHCI3 with 0.5% AcOH to give 141 mg (28%) of the title compound as a white foamy solid. ¹ H NMR (CDCI3, 300 MHz) mixture of amide rotamers δ 1.30 - 1.50 (m, 4H), 2.05 (s, 2H), 2.36 (bs, 1H), 2.60 (bs, 1H), 3.0 (bs, 1H), 3.4 (bs, 1H), 3.8 (bs, 1H), 4.66 (bs, 1H), 6.90 - 7.40 (m, 13H). MS (CI-NH ₃ ) m/e M+, 431.

Example 155 4-rN-Benzyl-N-(5-phenylpentvπaminocarbonylmethyl]benzene-1. 2-dicarboxylic acid

Example 155A N-Benzyl-5-phenylpentanamide To a solution of 5-phenylpentanoic acid (4.43 mmol) in 20 mL of CH2CI2 at room temperature was added oxalyl chloride (1.41 g, 11.08 mmol) and 1 drop of DMF, and the reaction mixture was stirred for 1 hour under nitrogen. The CH2CI2 and excess oxalyl chloride were evaporated. The reaction was redissolved in CH2CI2 and benzylamine (1.42 g, 13.3 mmol) and 2 mL of saturated aqueous NaHC03 were added and the reaction stirred for 12 hours. The reaction was taken up in EtOAc and washed with 1 N HCI x 2, followed by saturated aqueous NaCI, and evaporated to give an oil that was used without further purification. ^" Η NMR (CDCI3, 300 MHz) δ 1.60 - 1.80 (m, 4H), 2.22 (t,

2H), 2.64 (t, 2H), 4.42 (d, 2H), 5.68 (bs, 1H), 7.12 - 7.35 (m, 10H) MS (CI-NH3) m/e MH+, 268.

Example 155B N-Benzyl-N-(5-phenylpentvπamine

The compound resulting from Example 155A (4.33 mmol) was dissolved in THF and lithium aluminum hydride (8.66 mL, 1.0 M solutionn in THF) was added by syringe, and the reaction was heated to reflux for 6 hours under nitrogen. The reaction was cooled and quenched with water and 15% aqueous sodium hydroxide, then filtered and evaporated to give the title compound as an oil that was used without further purification. ¹ H NMR (CDCI3, 300 MHz) δ 1.31 - 1.46 (m, 2 H), 1.50 - 1.68 (m, 4H), 2.57 - 2.65 (app q, 4H), 3.78 (s, 2H), 7.14 - 7.34 (m, 10 H). MS (DCI-NH3) m/e 254 (M+H)+.

Example 155C

4-fN-Benzyl-N-f5-phenylpentvπaminocarbonylmethyllbenzene -1.2-dicarboxylic acid The title compound was prepared using the compound resulting from Example 155B and the procedures described in Example 154B. ¹ H NMR (CDCI3, 300 MHz) mixture of amide rotamers δ 1.30 - 1.50 (m, 4H), 1.52 - 1.62 (m, 2H), 2.45 (bs, 1H), 2.55 (bs, 1H), 3.0 (bs, 1H), 3.4 (bs, 1H), 4.35 (bs, 1H), 4.71 (bs, 1H), 6.97 - 7.40 (m, 12H), 7.8 (bs, 1H). MS (DCI-NH ₃ ) m/e 445 (M+H)+.

Example 156

2-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1benzene-1.3- dicarboxylic acid

Example 156A 3-Carboxyphthalic anhydride Benzene 1 ,2,3-tricarboxyIic acid monohydrate (20 g, 95.2 mmol) and acetic anhydride (13.5 mL, 143 mmol) were stirred at reflux for 4 hours. The solution was cooled to 0 °C and the resulting white solid was collected by filtration and then Soxhlet-extracted over 48 hours with diethyl ether. The

filtrate was dried under high vacuum at 60 °C to provide 16.7 g (91%) of the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 7.5 (dd, 1H), 7.8 (dd, 1H), 8.1 (dd, 1H), 12.5 (br s, 1H). MS (DCl) m/e 210 (M+NH ₄ ) ⁺ .

Example 156B

3-Chloroformylphthalic anhydride The compound resulting from Example 156A (6.34 g, 32.9 mmol) was treated with thionyl chloride (3.3 mL, 53.8 mmol) and DMF (6 μL) then stirred at 115 °C for 3 hours. Removal of volatiles under reduced pressure provided 6.9 g of the crude title compound as a yellow solid which was used without further purification in the next step.

Example 156C 2.6-Dimethoxycarbonylbenzoic acid (\) and 2.3-Dimethoxycarbonylbenzoic acid (\\)

A solution of the compound resulting from Example 156B (6.9 g, 32.9 mmol) and diisopropylethylamine (17.2 mL, 98.7 mmol) in methanol (66 mL) was stirred at -20 °C for 9 hours then at room temperature for 18 hours. The volatiles were removed under reduced pressure, and the residue was partitioned between ethyl acetate (100 mL) and 6 f_J HCI (100 mL). The organic layer was dried (MgS04), filtered, treated with silica gel and concentrated to dryness. The mixture was slurried in 98:1:1 CHCl3-MeOH-HOAc and added to a column made up of the same solvent system. Elution with 98:1:1 CHCI3- MeOH-HOAc provided 3.09 g (39%) of 2,6-dimethoxycarbonylbenzoic acid (i) and 1.74-g (22%) of 2,3-dimethoxycarbonylbenzoic acid (ii). ¹ H NMR (i)

(CDCI3/3OO MHz) δ 3.5 (s, 6H), 7.2 (dd, 1H), 7.6 (dd, 1H), 7.9 (dd, 1 H), 12.5 (br s, 1H). MS (DCl) m/e 239 (M+H)+. 1H NMR (ii) (CDCI ₃₎ 300 MHz) δ 3.5 (s, 3H), 3.6 (s, 3H), 7.5 (dd, 1H), 7.9 (dd, 1H), 8.0 (dd, 1H), 12.5 (br s, 1H). MS (DCl) m/e 239 (M+H)+.

Example 156D 2-fN-Benzyl-N-(4-phenoxybenzvπaminocarbonvπ-1.3- dimethoxycarbonylbenzene A solution of the compound resulting from Example 156C (i) (543 mg, 2.3 mmol) in toluene (5 mL) was treated with oxalyl chloride (258 μL) and DMF (2 drops). After 18 hours, the solution was concentrated to dryness, and the acid chloride thus obtained was dissolved in CH2CI2 (5 mL) and added dropwise to a slurry of N-benzyl-N-(4-phenoxybenzyl)amine hydrochloride (1.1 g, 3.42 mmol) and NaHC03 (1.21 g, 11.4 mmol) in water (10 mL). After 18 hours, the CH2CI2 layer was dried (MgS04), filtered and concentrated. The residue was chromatographed on silica gel eluting with 15% ethyl acetate in hexane to provide 1.13 mg (97%) of the title compound as a white foam. ¹ H NMR (CDCI3, 300 MHz) δ 3.9 (s, 6H), 4.7 (s, 2H), 5.6 (s, 2H), 7.0-7.4 (m, 14H), 7.6 (dd, 1H), 7.8 (dd, 1H), 8.0 (dd, 1H). MS (DCl) m/e 510 (M+H)+.

Example 156E 2-fN-Benzyl-N-f4-phenoxybenzyl)aminocarbonvnbenzene-1.3-dica rboxylic acid

A solution of the compound resulting from Example 156D (1.1 g, 2.3 mmol) in THF (34 mL) and water (11 mL) was treated with 1 N LiOH (11.4 mL). After 18 hours, the solution was acidified to pH 0 with 1 U HCI, concentrated to remove the THF, and extracted with ethyl acetate. The combined organic extracts were dried (MgSθ4), filtered and concentrated, and the residue was chromatographed on silica gel eluting with 98:1:1 CHCl3-MeOH-HOAc to provide 87 mg (80%) of the title compound as a white solid. H NMR DMSO-d6, 300 MHz) δ 4.7 (s, 2H), 5.8 (s, 2H), 7.1-7.4 (m, 14H), 12.5 (br s, 2H). MS (DCl) m/e 482 (M+H)+.

Example 157 2-fN-Benzyl-N-(4-phenoxybenzvπaminocarbonylamino1benzene-1. 3- dicarboxylic acid

Example 157A

N-Benzyl-N-f4-phenoxybenzyn-N'-f2.6-dimethoxycarbonylphen yπurea A solution of 2,6-dimethoxycarbonylbenzoic acid (500 mg, 2 mmol) in toluene (5 mL) was treated with diphenylphosphoryl azide (643 mg, 2.34 mmol) and triethylamine (644 mg, 2.3 mmol) then warmed to 80 °C for 18 hours. N- Benzyl-N-(4-phenoxybenzyl)amine hydrochloride (1.04 g, 3.2 mmol) was added, and the brown reaction mixture was stirred an additional 18 hours. Volatiles were removed, and the residue was dissolved in ethyl acetate and washed with 1 £J HCI, half saturated NaHCOβ and brine, dried (MgSθ4), filtered, concentrated, and chromatographed on silica gel eluting with 15% ethyl acetate in hexane to provide 99 mg (90%) of the title compound as a colorless oil. ¹ H NMR (CDCI3, 300 MHz) δ 3.4 (s, 6H), 4.6 (s, 2H), 5.1 (s, 2H), 6.1 (brs, 1H), 7.2-7.5 (m, 14H), 7.6 (dd, 1H), 7.8 (dd, 1H), 8.0 (dd, 1H). MS (DCl) m/e 525 (M+H)+.

Example 157B

2-[N-Benzyl-N-(4-phenoxybenzyπaminocarbonylamino1benzenB -1.3- dicarboxylic acid The compound resulting from Example 157A (99 mg, 2.1 mmol) in water (10 mL) and THF 32 mL) was treated with 1 N LiOH (10.6 mL) and stirred for 18 hours. The solution was acidified to pH 0 with 1 N HCI and concentrated to remove THF. The water layer was extracted with ethyl acetate. The combined organic extracts were dried (MgSθ4), filtered and concentrated to provide a residue which was chromatographed on silica gel eluting with 98:1:1 CHCI ₃ - MeOH-HOAc to provide 90 mg (96%) of the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 4.7 (s, 2H), 5,2 (s, 2H), 6.3 (br s, 1 H), 7.2-7.5 (m,

14H), 7.6 (dd, 1H), 7.8 (dd, 1H), 8.1 (dd, 1H), 12.7 (brs, 2H). MS (DCl) m/e 497 (M+H)+.

Example 158 3- ^r N-Benzyl-N-(4-phenoxybenzyhaminocarbonylamino ^' [benzene-1.2- dicarboxylic acid dimethyl ester A solution of 2,3-dimethoxycarbonylbenzoic acid (421 mg, 1.7 mmol) in toluene (5 mL) was treated with diphenylphosphoryl azide (535 mg, 1.94 mmol) and triethylamine (358 mg, 3.54 mmol) and then warmed to 80 °C for 18 hours. N-Benzyl-N-(4-phenoxybenzyl)amine hydrochloride (577 mg, 1.77 mmol) was added, and the brown reaction mixture was stirred an additional 18 hours. The volatiles were removed, and the residue was dissolved in ethyl acetate and washed with 1 HCI, half saturated NaHC0 ₃ and brine, dried (MgSθ4), filtered, concentrated and chromatographed on silica gel eluting with 20% ethyl acetate in hexane to provide 750 mg (83%) of the title compound as a colorless glass. 1H NMR (CDCI ₃ , 300 MHz) δ 3.5 (s, 3H), 3.6 (s, 3H), 4.4 (s, 2H), 5.6 (s, 2H), 6.2 (s, 1H), 7.0-7.7 (m, 14H), 7.2 (dd, 1H), 7.6 (dd, 1H), 7.9 (dd, 1H). MS (DCl) m/e 525 (M+H)+.

Example 159 4-r2-Benzyl-3-^4-phenoxyphenvπpropionylamino1benzene-1.2-di carboxylic acid

Example 159A

Methyl 4-phenoxycinnamate A slurry of NaH (4 g, 0.1 mol) in THF (200 mL) at 0 °C was treated with methyl dimethylphosphonoacetate (21.8 g, 0.12 mol) and stirred for 1 hour. 4- Phenoxybenzaldehyde was added to the mixture, and stirring was continued for 18 hours. The volatiles were removed under reduced pressure, and the residue was partitioned between water (500 mL) and ethyl acetate (500 mL). The organic phase was dried (MgS04), filtered and concentrated to provide a white solid which was used directly in the next step without further purification. 1H NMR (CDCI3. 300 mHz) δ 3.7 (s, 3H), 6.25 (s, 1H), 6.51 (s, 1H), 6.9-7.1 (m, 4H), 7.1-7.3 (m, 3H), 7.3-7.4 (m, 2H). MS (DCl) m/e 255 (M+H)+.

Example 159B Methyl 3-f4-phenoxyphenyπpropionate A mixture of the compound resulting from Example 159A (20 g, 78.7 mmol), ammonium formate (49.5 g, 786 mmol) and 10% Pd on carbon (5 g) was warmed to reflux for 18 hours, cooled to room temperature, filtered through celite, concentrated and chromatographed eluting with 10% ethyl acetate in hexane to provide 20 g (99%) of the title compound as a colorless oil. ¹ H NMR (CDCI ₃ , 300 MHz) δ 2.6 (t, 2H), 2.9 (t, 2.9), 3.7 (s, 3H), 6.9-7.1 (m, 4H), 7.1-7.3 (m, 3H), 7.3-7.5 (m, 2H). MS (DCl) m/e 257 (M+H)+.

Example 159C Methyl 2-benzyl-3-(4-phenoxyphenyI)propionate A solution of 0.5 M potassium bis(trimethylsilyl)amide in toluene (36.7 mL) at -78 °C was treated via cannula with a -78 °C solution of the compound resulting from Example 159B (4.3 g, 16.7 mmol) in THF (56 mL). After 1 hour at -78 °C, the enolate solution was treated with benzyl bromide (11.43 g, 66.8 mmol) and stirred for an additional 10 hours. The reaction was quenched with saturated ammonium chloride, and the THF was removed under reduced pressure. The water layer was extracted with ethyl acetate (2 x 100 mL), and the combined extracts were dried (MgS04), filtered and concentrated. The residue was chromatographed eluting with 10% ethyl acetate in hexane to provide 800 mg (14%) of the title compound as a colorless oil. ¹ H NMR (CDCI ₃₎ 300 MHz) δ 2.8 (m, 2H), 3.0 (m, 3H), 3.5 (s, 3H), 6.9-7.4 (m, 14H). MS (DCl) m/e 347 (M+H)+.

Example 159D 2-Benzyl-3-f4-phenoxyphenv0propionic acid The compound resulting from Example 159C in methanol (12 mL) and water (3 mL) at room temperature was treated with 87% KOH (680 mg, 10.4 mmol) in water (5 mL). After 18 hours, the solution was acidified to pH 0 with 1 N HCI and concentrated to remove methanol. The resulting suspension was extracted with ethyl acetate (2 x 25 mL). The combined organic extracts were dried (MgS04), filtered and concentrated to provide an oil which was used in

the next step without further purification. ¹ H NMR (CDCI3, 300 MHz) δ 2.7 (m, 2H), 3.1 (m, 3H), 6.9-7.4 ( , 14H), 12.0 (br s, 1H). MS (DCl) m/e 333 (M+H)+.

Example 159E 4-f2-Benzyl-3-(4-phenoxyphenvflpropionylamino1benzene-1.2-di carboxylic acid dimethyl ester A solution of the compound resulting from Example 159D (774 mg, 2.3 mmol) in toluene (2 mL) was treated with oxalyl chloride (32.5 mg, 2.6 mmol) and DMF (2 drops). After 18 hours, the solution was concentrated to dryness, and the acid chloride thus obtained was dissolved in CH2CI2 (5 mL) and added dropwise to a slurry of 4-amino-1 ,2-dimethylphthalate (562 mg, 2.3 mmol) and NaHC03 (247 mg, 2.3 mmol) in water (5 mL). After 18 hours, the CH2CI2 layer was dried (MgSθ4), filtered and concentrated. The residue was chromatographed on silica gel eluting with 25% ethyl acetatein hexane to provide 1.01 g (83%) of the title compound as a colorless glass. ¹ H NMR

(CDCI3, 300 MHz) δ 2.7 (m, 1H), 2.9 (m, 2H), 3.05 (m, 2H), 3.85 (s, 3H), 3.86 (s, 3H), 6.8 (s, 1H), 7.05-7.45 (m, 16H), 7.65 (d, 1H). MS (DCl) m/e 524 (M+H)+.

Example 159F 4-[2-Benzyl-3-(4-phenoxyphenvnpropionylamino]benzene-1.2-dic arboxylic acid A solution of the compound resulting from Example 159E (90 mg, 0.17 mmol) in MeOH (1 mL) and water (1 mL) at room temperature was treated with 87% KOH (109 mg, 1.7 mmol) in water (1 mL). After 18 hours, the methanol was removed, and the aqueous solution was acidified to pH 0 with 1 J HCI. The suspension was extracted with ethyl acetate (2 x 5 mL), and the combined extracts were dried (MgSθ4), filtered and concentrated. The residue was chromatographed on silica gel eluting with 98:1:1 CHCl3-MeOH-HOAc to provide 80.3 mg (95%) of the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 2.7 (m, 2H), 3.0 (m, 3H), 6.8 (m, 5H), 7.1-7.4 (m, 10H), 7.5-7.7 (m, 3H), 12.0 (br s, 2H). MS (DCl) m/e 496 (M+H)+.

Example 160 4-fN-Benzyl-N-(4-phenoxybenzyπaminocarbonyl ^' |benzene-1.3-dicarboxylic acid

Example 160A 4-Nitrobenzene-1.3-dicarboxylic acid

Potassium permanganate (262 g, 1.66 mol) was added portionwise over 6 hours to a solution of of 5-methyl-2-nitrobenzoic acid (112.3 g, 0.62 mol) and sodium hydroxide (25.6 g, 0.62 mol) in water (1.5 L) at 90 °C. After 6 hours, the solution was cooled to room temperature and filtered through celite. The filtrate was acidified with 12 H HCI (500 mL) and refrigerated for 18 hours. The resulting solid was filtered and dried under high vacuum to provide 128.7 g (98%) of the title compound as a white solid. ¹ H NMR (DMSO-d ₆ , 300 MHz) δ 8.25 (dd, 1H), 8.8 (d, 1H), 8.9 (d, 1H), 12.0 (br s, 2H). MS (DCl) m/e 212 (M+H)+.

Exampe 160B Dimethyl 4-nitroisophthalate The compound resulting from Example 160A (96 g, 0.455 mol) in methanol (1L) was treated with concentrated sulfuric acid (100 mL) and refluxed for 18 hours. The volume of methanol was reduced to -200 mL, and the hot solution was treated with water until turbid. The suspension was refrigerated for 18 hours and then filtered to provide 93 g (85%) of the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 3.8 (s, 3H), 3.85 (s, 3H), 8.25 (dd, 1H), 8.8 (d, 1H), 8.9 (d, 1H). MS (DCl) m e 240 (M+H)+.

Example 160C Dimethyl 4-aminoisophthalate A slurry of the compound resulting from Example 160B (20 g, 83.7 mmol), ammonium formate (52 g, 837 mmol) and palladium on carbon (5 g) in methanol (500 mL) was stirred at reflux for 3 hours, cooled, filtered and concentrated to provide the title compound as a yellow solid which was used in the next step without further purification. ¹ H NMR (CDCI ₃ , 300 MHz) δ 3.8 (s,

3H), 3.85 (s, 3H), 4.2 (s, 2H), 8.0 (dd, 1H), 8.6 (dd, 1H), 8.8 (d, 1H). MS (DCl) m/e 210 (M+H)+.

Example 160D Dimethyl 4-iodoisophthalate

A slurry of the compound resulting from Example 160C (8.5 g, 40.7 mmol) in 2% HCI (150 mL) at 0 °C was treated with sodium nitrite (3.1 g, 44.8 mmol) followed by potassium iodide (10.1 g, 61.1 mmol). The brown slurry was stirred for 1 hour at 0 °C and then treated with sodium sulfite (300 g). The yellow slurry was extracted with ethyl acetate (3 x 250 mL), and the combined extracts were dried (MgS04), filtered and concentrated to provide a yellow oil. Chromatography of the oil on silica gel eluting with ethyl acetate/hexane provided 5.1 g (39%) of the title compound as a white solid. ¹ H NMR (CDCI3, 300 MHz) δ 3.8 (s, 3H), 3.85 (s, 3 H), 7.9 (dd, 1 H), 8.6 (dd, 1 H), 8.7 (d, 1 H). MS (DCl) m/e 321 (M+H)+.

Example 160E Dimethyl 4-vinylisophthalate A solution of the compound resulting from Example 160D (2.0 g, 6.2 mmol), vinyltributyltin (2.4 g, 7.5 mmol) and tetrakistriphenylphosphine- palladium(O) (36 mg) were stirred in toluene (30 mL) at 100 °C for 18 hours. The solution was then cooled to 0 °C, treated with 1:1 water-DBU (10 mL) and filtered through celite. The layers were separated, and the organic fraction was dried (MgS04), filtered and concentrated. The residue was chromatographed on silica gel eluting with 5% ethyl acetate in hexane to provide 620 mg (48%) of the title compound as a white solid. ¹ H NMR (CDCI ₃ ) δ 3.95 (s, 3H), 4.0 (s, 3H), 5.45 (dd, 1H), 5.8 (dd, 1H), 7.7 (m, 1H), 7.9 (dd, 1H), 8.6 (dd, 1H), 8.7 (d, 1H). MS (DCI) m/e 221(M+H)+.

Example 160F 2.4-Dimethoxycarbonylbenzoic acid A suspension of the compound resulting from Example 160E (302 mg, 1.4 mmol), ru ienium trichloride (14.2 mg, 0.069 mmol) and sodium periodate (1.46 g, 6.85 mmol) in 1:1 :1.5 CCI4-CH3CN-H2O (10.5 mL) was stirred at 60 °C for 2 hours. The reaction mixture was cooled to room temperature and filtered through celite. The layers were separated, and the organic layer was dried (MgSθ4), filtered and concentrated. The white residue thus obtained (326 mg, 99%) was used without further purification in the next step. ¹ H NMR (CbCl3, 300 MHz) δ 3.8 (s, 3H), 3.9 (s, 3H), 8.2 (dd, 1H), 8.7 (dd, 1H), 8.8 (dd, 1H), 12.0 (br s ^' , 1H). MS (DCl) m/e 239 (M+H)+.

Example 160G 4-fN-Benzyl-N-(4-phenoxybenzyπaminocarbonyl]-1.3- dimethoxycarbonylbenzene

A solution of the compound resulting from Example 160F (286 mg, 1.2 mmol) in toluene (4 mL) was treated with oxalyl chloride (184 mg, 1.4 mmol) and DMF (2 drops) then stirred for 18 hours. The toluene was removed, and the acid chloride thus obtained was dissolved in CH2CI2 (5 mL) and added to a slurry of N-benzyl-N-(4-phenoxybenzyl)amine (347 mg, 1.2 mmol) and NaHC03 (1-27 g, 12 mmol) in water (5 mL). After 18 hours, the layers were separated, and the organic layer was dried (MgS04), filtered, concentrated and chromatographed eluting with 20% ethyl acetate in hexane to provide 600 mg (99%) of the title compound as a colorless glass. ¹ H NMR (CDC3, 300 MHz) δ 3.8 (s, 3H), 3.85 (s, 3H), 4.6 (s, 2H), 4.65 (s, 2H) 7.0-7.4 (m, 14H), 8.2 (dd, 1 H), 8.65 (d, 1H), 8.8 (d, 1H). MS (DCl) m e 510 (M+H)+.

Example 160H 4-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl1benzene-1.3-dic arboxylic acid

A solution of the compound resulting from Example 160G (600 mg, 1.2 mmol) in water (1 mL) and MeOH (3 mL) was treated with 87% KOH (76 mg, 11.8 mmol) in water (1 mL) and stirred for 18 hours. The MeOH was removed, and the resulting suspension was extracted with ethyl acetate (2 x 5 mL). The ethyl acetate was dried (MgS04), filtered and concentrated to provide an oil. Chromatography of the oil eluting with 98:1:1 CHCl3-MeOH-HOAc provided 560 g (98%) of the title compound as a white solid. ¹ H NMR (DMSO-dβ, 300 MHz) δ 4.5 (s, 2H), 4.55 (s, 2H), 7.0-7.4 (m, 14H), 8.1 (dd, 1H), 8.6 (d, 1H), 8.8 (d, 1H), 12.0 (br s, 2H). MS (DCl) m/e 482 (M+H)+.

Example 161 4-fN-Benzyl-N-f4-phenoxybenzyπaminocarbonylamino ^' |benzene-1.3- dicarboxylic acid

Example 161 A

4-[N-Benzyl-N- ⁽ 4-phenoxybenzvπaminocarbonylamino1benzene-1.3- dicarboxylic acid dimethyl ester A solution of 2,4-dimethoxycarbonylbenzoic acid (301 mg, 1.3 mmol) in toluene (4 mL) was treated with diphenylphosphoryl azide (383 mg, 1.4 mmol) and triethylamine (255 mg, 2.5 mmol) and stirred 18 hours at 80 °C. N-Benzyl- N-(4-phenoxybenzyl)amine (472 mg, 1.63 mmol) was then added to the reaction mixture, and stirring was continued for an additional 18 hours. The reaction mixture was cooled to room temperature, washed with 1 £J HCI (10 mL), half-saturated NaHC03 (10 mL) and brine (10 mL), and concentrated to dryness. The residue was chromatographed on silica gel eluting with 20% ethyl acetate in hexane to provide 650 mg (98%) of the title compound as a colorless glass. ¹ H NMR (CDCI ₃ , 300 MHz) δ 3.8 (s, 3H), 3.85 (s, 3H), 3.95 (s, 2H), 4.0 (s, 2H), 4.1 (d, 1H), 6.9-7.45 (m, 14H), 8.2 (m, 2H), 8.65 (dd, 1H). MS (DCl) m/e 525 (M+H)+.

Example 161 B 4-fN-Benzyl-N-f4-Dhenoxybenzyπaminocarbonylamino1benzene-1. 3- dicarboxylic acid A solution of the compound resulting from Example 161 A (650 mg, 1.2 mmol) in water (1 mL) and MeOH (3 mL) was treated with 87% KOH in water (1 mL) and stirred for 18 hours. The MeOH was removed, and the resulting suspension was extracted with ethyl acetate (2 x 5 mL). The ethyl acetate was dried (MgSθ4), filtered and concentrated to provide an oil. Chromatography of the oil eluting with 98:1 :1 CHCI ₃ -MeOH-HOAc provided 600 mg (98%) of the title compound as a white solid. ¹ H NMR δ (DMSO-d ₆ , 300 MHz) δ 3.99 (s, 2H), 4.0 (s, 2 ^' H), 4.3 (d, 1H), 6.85-7.45 (m, 14H), 8.2 (m, 2H), 8.65 (m, 1H), 12.6 (brs, 2H). MS (DCl) m/e 497 (M+H)+.

Example 162 5-[N-(2-Pyridylmethyπ-N-f4-phenoxybenzvπaminocarbonvnbenze ne-1.2.4- tricarboxylic acid Using the procedures described in Example 1 and substituting 2- pyridylmethylamine for benzylamine provided the title compound. ¹ H NMR (DMSO-de, 500 MHz) δ 4.3 (m, 4H), 6.85-8.5 (m, 15H). MS m/e 527 (M+H)+.

Example 163 5-[N-(3-Pyridylmethvπ-N-f4-phenoxybenzvπaminocarbonvnbenze ne-1.2.4- tricarboxylic acid Using the procedures described in Example 1 and substituting 3- pyridylmethylamine for benzylamine provided the title compound. ¹ H NMR (DMSO-de, 500 MHz) δ 4.3 (m, 4H), 6.85-8.55 (m, 15H). MS m/e 527 (M+H)+.

Example 164 5-fN-(4-Pyridylmethv0-N-f4-phenoxybenzvπaminocarbonyl1benze ne-1.2.4- tricarboxylic acid

Using the procedures described in Example 1 and substituting 4- pyridylmethylamine for benzylamine provided the title compound. ¹ H NMR (DMSO-de, 500 MHz) δ 4.28 (bs, 4H), 6.83-8.54 (m, 15H). MS m/e 527 (M+H)+.

Example 165 4-fN-Benzyl-N-f3-phenoxybenzvhamiπocarbonyllbenzene-1.2-dic arboxylic acid A solution of triethylamine (0.51 g, 0.005 mol) and N-benzyl-N-(3- phenoxybenzyl)amine (1.45 g, 0.005 mol) in THF (30 mL) was added dropwise to a stirred solution of 1.05 g (0.005 mol) of 4-chloroformylphthalic anhydride, prepared by the method described in J. Org. Chem. 38: 2257 (1973), in THF (30 mL) at 0 °C. After the addition was complete, the reaction mixture was slowly allowed to come to ambient temperature ovemight. It was then concentrated in vacuo at 20 °C. The residue obtained was treated with methylene chloride (10 mL) and 6 N HCI (90 mL) and stirred at ambient temperature for 2 hours. The organic layer was removed, and the aqueous layer was extracted with ethyl acetate (3 x 50 mL). The combined organic extracts were washed successively with 5% HCI, cold water and saturated sodium chloride solution, dried over magnesium sulfate and evaporated under reduced pressure to give a viscous liquid. Chromatography on silica gel eluting with 84.5:13.5:2 CHCl3-MeOH- HOAc afforded the product as a mono-methyl ester. The ester was hydrolyzed by refluxing ovemight with lithium hydroxide (1.8 g, 0.043 mol) in THF (20 mL) and water (12 mL). The mixture was evaporated to remove the THF, diluted with cold water (20 mL), acidified with dilute HCI and extracted with ethyl acetate (3 x 50 mL). The combined organic extracts were washed with cold water and saturated sodium chloride solution, dried over sodium sulfate, evaporated and chromatographed on silica gel eluting with 95:2.5:2.5 ethyl acetate-acetic acid-water to give 1.6 g (65%) of the title compound. ¹ H NMR (CDCI ₃ , 300 MHz) δ 2.35 (s, 1 H), 4.30 (d, 2H), 4.7 (d, 2H), 6.7-7.85 (m, 17H), 9.5 (s, 2H). MS (DCI/NH ₃ ) m/e 499 (M+NH ₄ ) ⁺ .

Example 166 4-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonyllbenzene-1.2-dic arboxylic acid The title compound was prepared by treating 1.5 g (0.005 mol) of N- benzyl-N-(4-phenoxybenzyl)amine and 0.51 g (0.005 mol) of triethylamine in 30 mL of THF with 1.05 g (0.005 mol) of 4-chloroformyl phthalic anhydride in THF (20 mL) by the method described for Example 165. The crude compound

was chromatographed on silica gel eluting with 95:2.5:2.5 ethyl acetate-acetic acid-water to give 1.5 g (61%) of the title compound as a cream colored amorphous solid. ¹ H NMR (CDCI ₃ , 300 MHz) δ 2.08 (d, 1H), 2.25 (q, 1H), 2.35 (s, 1H), 2.5 (t, 1H), 4-5 (m, 7H), 6.9-7.8 (m, 17H). MS (DCI/NH3) m/e 496 (M+H) ⁺ , 513 (M+H+NH3) ^4* .

Example 167

5-fN-M .2.3.4-Tetrahvdronaphth-1 -vIVN-f 4- phenoxybenzvπaminocarbonyribenzene-1.2.4-tricarboxylic acid

Example 167A N-te-Phenoxybenzvh-N-f 1.2.3.4-tetrahvdronaphth-1 -vnamine A solution of 4-phenoxybenzaldehyde (13.5 g, 0.068 mol) and (1,2,3,4- tetrahydronaphth-1-yl)amine (10.02 g, 0.068 mol) in absolute ethanol (150 mL) containing a few crystals of p-toluenesulfonic acid was refluxed. After 4 hours, the reaction mixture was cooled, and NaBH4 (2.6 g, 0.068 mol) was added portionwise over 15-20 minutes. The mixture was then heated at reflux for an additional 4 hours. The volatiles were removed under reduced pressure, and the residue was triturated with ice-water (200 mL) and extracted with ethyl acetate (4 x 80 mL). The combined organic extracts were washed with brine (3 x 50 mL), dried over anhydrous magnesium sulfate, filtered and evaporated in vacuo. The residue was purified by column chromatography eluting with 92:8:1 hexane-ethyl acetate-triethylamine to give 17.03 g (76%) of the title compound as a colorless viscous liquid. ¹ H NMR (CDCI3, 300 MHz) δ 7.8-6.8 (m, 13H), 3.92 (d, 1 H), 3.82 (t, 2H), 2.8 (m, 2H), 1.95 (m, 3H), 1.75 (m, 1 H), 1.4 (s, 1 H). MS (DCI/NH3) m/e 330 (M+H) ⁺ . Anal calcd for C23H23NO: C, 83.85; H, 7.04; N. 4.25. Found: C, 84.02; H, 7.11; N, 4.28.

Example 167B 5-fN-M .2.3.4-Tetrahydronaphth-1 -vH-N-M- phenoxybenzvflaminocarbonyl]benzene-1 ,2.4-tricarboxylic acid A solution of N-(4-phenoxybenzyl)-N-(1 ,2,3,4-tetrahydronaphth-1- yl)amine (0.99 g, 0.003 mol) in anhydrous THF (40 mL) was added dropwise,

under a nitrogen atmosphere, to a stirred solution of 1 ,2,4,5- benzenetetracarboxylic dianhydride (0.76 g, 0.003 mol) in THF (80 mL) containing triethylamine (0.31 g, 0.003 mol) at -30 °C. After 2 hours, the cooling bath was removed. After 1.6 hours at ambient temperature, the solvent was removed under reduced pressure at 20 °C. The residue was taken up in ethyl acetate, washed with 10% HCI (30 mL) and brine (30 mL), dried over anhydrous sodium sulfate, filtered and concentrated in vacuo. The residue was taken up in 80 mL of THF, stirred for 1 hour with saturated sodium carbonate solution (20-30 mL) and stirred an additional hour at ambient temperature. The THF was removed under reduced pressure, and the residue was diluted with cold water (50 mL), cooled and acidified with 10% HCI. The desired product was extracted with ethyl acetate (3 x 30 mL). The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated in vacuo. The residue obtained was purified by column chromatography eluting with 95:1.25:1.25:2.5 ethyl acetate-formic acid-water-methanol followed by 180:1:1 ethyl acetate-formic acid-water to give the title compound (0.5 g, 30%) as a white amorphous solid. ¹ H NMR (DMSO-de, 300 MHz) δ 7.9-6.7 (nm 15H), 5.6 (s, 1H), 4.95 (d, 1H), 4.7 (d, 2H), 4.7 (m, 1H), 3.5 (s, 2H), 2.62 (d, 1H), 2.5 (s, 1H), 2.0 (s, 1H), 1.7 (s, 1H), 1.25 (s, 1H). MS (FAB+) m/e 566 (M+H) ⁺ and (FAB") 564. Anal calcd for C33H27NO8: C, 70.08; H, 4.80; N, 2.48. Found: C, 69.90; H, 4.83; N, 2.23.

Example 168 5-[N-f1-Phenyl-2-f4-phenoxyphenvπethyl)aminocarbonvπbenzen e-1.3- dicarboxylic acid

A mixture of 4-phenoxyphenyl benzyl ketone (5.5 g, 0.019 mol) and benzylamine (2.04 g, 0.019 mol) in methanol (250 mL) was shaken with 5% platinum on carbon (1.1 g) for 16 hours and then for 24 hours with hydrogen at 4 atmospheres. The reaction mixture was filtered, evaporated under reduced pressure and then under high vacuum to give 2-(4-phenoxyphenyl)-1-phenyl-1- aminoethane (7.0 g, 97%) as a colorless solid.

A solution of the above amine (0.54 g, 0.0014 mol) and triethylamine (0.19 mL, 0.0014 mol) in anhydrous methylene chloride was stirred at ambient

temperature while a solution of dimethyl-5-carbonyl chloride-1,3-benzene dicarboxylate (0.33 g, 0.0013 mol), prepared by the method described in Photochemistry and Photobiology 51: 155 (1990), in methylene chloride (15 mL) was added dropwise under a nitrogen atmosphere. After stirring overnight at ambient temperature, the mixture was evaporated under reduced pressure at 20 °C. The solid residue was chromatographed on silica gel eluting with 8:2 hexane-ethyl acetate to give 442 mg (57%) of 1-benzyl-1-(4-phenoxyphenyI)-1- (3,5-dimethoxycarbonyl-1-benzamido)methane.

The above diester (0.36 g, 0.0006 mol) was refluxed ovemight with lithium hydroxide (0.198 g, 0.0048 mol) in a mixture of methanol (9 mL) and water (7 mL). The reaction mixture was cooled, diluted with cold water, acidified with HCI and extracted with ethyl acetate (3 x 40 mL). The combined organic extracts were washed with saturated sodium chloride solution, dried over sodium sulfate and evaporated under reduced pressure togive the title compound as a colorless amorphous solid. ¹ H NMR (CDCI3, 300 MHz) δ 4.40 (d, 2H), 4.75 (d, 1H), 6.78-7.4 (m, 17H), 8.4 (s, 2H), 8.85 (s, 1H). MS (DCI/NH ₃ ) m/e 482 (M+H) ⁺ , 499 (M+H+NH ₃ ) ⁺ .

Example 169 5-f N-M .2.3.4-Tetrahvdronaphth-1 -vH-N-(3- phenoxybenzyπaminocarbonyπbenzene-1.2.4-tricarboxylic acid

Example 169A N-π.2.3.4-Tetrahvdronaphth-1-vπ-N-(3-phenoxybenzvπamine A solution of 3-phenoxybenzaldehyde (13.5 g, 0.068 mol) and 1,2,3,4- tetrahydronaphth-1-ylamine (10.02 g, 0.068 mol) in absolute ethanol (150 mL) containing a few crystals of p-toluenesulfonic acid was refluxed. After 4 hours, the reaction mixture was cooled and NaBH4 (2.6 g, 0.068 mol) was added portionwise over 20 minutes with stirring. The reaction mixture was again brought to reflux for an additional 4 hours and then concentrated under reduced pressure. The residue obtained was treated with ice-water (200 mL) and extracted with ethyl acetate (4 x 80 mL). The combined organic extracts were washed wtih brine (3 x 50 mL), dried over magnesium sulfate, filtered and

evaporated in vacuo. The residue was purified by column chromatography eluting with 92:8:1 hexane-ethyl acetate-triethylamine to give 8.0 g (36%) of the title compound as a light yellow viscous liquid. MS (DCI/NH3) m/e 330 (M+H) ⁺ . Anal calcd for C23H23NO: C, 83.85; H, 7.04; N, 4.25. Found: C, 83.94; H, 7.08; N. 4.19.

Example 169B

5-fN-M .2.3.4-Tetrahvdronaphth-1 -yn-N-f3- phenoxybenzvπaminocarbonyllbenzene-1.2.4-tricarboxylic acid A solution of the compound resulting from Example 169A (0.99 g, 0.003 mol) in anhydrous THF (40 mL) was added dropwise over two hours under a nitrogen atmosphere to a stirred solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.76 g, 0.003 mol) in THF (80 mL) containing triethylamine (0.31 g, 0.003 mol) at a temperature of from about -30 °C to about -35 °C. The cooling bath was then removed. After 2 hours at ambient temperature, the solvent was removed under reduced pressure at 20 °C. The residue was taken up in ethyl acetate, washed successively with 10% HCI (2 x 25 mL) and brine (2 x 25 mL), dried over anhydrous sodium sulfate, filtered and evaporated. The residue was stirred at ambient temperature with a saturated sodium carbonate solution (20- 30 mL) and THF (80 mL). After 1 hour, the THF was removed under reduced pressure, and the residue was diluted with cold water (30-40 mL) and acidified with 10% HCI. This mixture was extracted with ethyl acetate, and the combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The residue was purified by column chromatography eluting with 180:1 :1 ethyl acetate-formic acidrwater followed by 1800:1 :1 ethyl acetate-formic acid-water to give the title compound (0.4 g, 24%). ¹ H NMR (DMSO-de, 500 MHz) δ 8.45-6.55 (m, 15H0), 5.52 (s, 1H), 4.8 (d, 1H), 4.65 (s, 1H), 4.1 (m, 1H), 3.9 (d, 2H), 2.6 (m, 2H), 2.5 (s, 1H), 2.05 (s, 1H), 1.7 (d, 1 H), 1.4 (s, 1 H). MS (FAB+) m/e 566 (M+H) ⁺ and (FAB") m/e 564 (M-H)". Anal calcd for C33H27NO8 ^• 0.75 H ₂ 0: C, 68.44; H, 4.96; N, 2.41. Found: C, 68.36; H, 5.08; N, 2.13.

Example 170 5-fN-f4-Chromanyπ-N-f4-phenoxybenzvπaminocarbonyl]benzene- 1.2.4- tricarboxylic acid

Example 170A

N-(4-Chromanvπ-N-(4-Dhenoxybenzyl)amine Hydrochloride A mixture of crude 4-amino chromane (2.9 g, 0.02 mol), prepared by reducing 4-chromanone oxime with Raney nickel in ethanol at 4 atmospheres of hydrogen for 51 hours at ambient temperature, and 4-phenoxybenzaldehyde (3.9 g, 0.02 mol) in absolute ethanol (50 mL) containing p-toluenesulfonic acid (0.1 g) was refluxed for 4 hours. The reaction mixture was then allowed to cool to ambient temperature and then treated portionwise with NaBH4 (0.8 g, 0.021 mol) with stirring. After the addition was complete, the mixture was refluxed for an additional 5 hours, cooled to ambient temperature and evaporated under reduced pressure. The residue was taken up in ethyl acetate and washed with 2 NaOH solution (2 x 40 mL) and brine (3 x 40 mL), dried over anhydrous sodium sulfate, filtered and concentrated in vacuo. The residue was dissolved in absolute ethanol, stirred with norite, filtered, cool, and acidified with concentrated HCI. The colorless solid obtained was recrystallized from absolute ethanol to give 3.6 g (50%) of the title compound, m.p. 180-182 °C. MS

(DCI/NH3) m/e 332 (M+H) ⁺ . Anal calcd for C22H21NO2 ^• HCI: C, 71.83; H, 6.03; N. 3.81. Found: C, 72.00; H, 5.96; N, 3.66.

Example 170B 5-fN-f4-Chromanyn-N-f4-phenoxvbenzvnaminocarbonvnbenzene-1.2 .4- tricarboxylic acid _. A suspension of the compound resulting from Example 170A (1.13 g, 0.003 mol) in anhydrous THF (60 mL) was added portionwise under a nitrogen atmosphere to a stirred solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.76 g, 0.003 mol) in anhydrous THF (60 mL) containing triethylamine (0.63 g, 0.006 mol) at -30 °C to -40 °C over approximately 2 hours. The cooling bath was removed, and after stim ^' ng for 3 hours at ambient temperature, the THF was removed under reduced pressure at 20 °C. The residue was taken up in ethyl

acetate, washed wtih 10% HCI and brine (30 mL), dried over anhydrous sodium sulfate, filtered and concentrated in vacuo. The residue obtained was diluted with cold water (30-40 mL), cooled, acidified with 10% HCI and extracted with ethyl acetate (3 x 30 mL). The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered, and evaporated in vacuo. The residue obtained was purified by chromatography eluting with 180:1:1 ethyl acetate-formic acid-water to give the title compound (1.26 g, 74%) as a colorless solid. ¹ H NMR (DMSO-de, 300 MHz) δ 8.4-6.74 (m, 15H), 5.68 (s, 1H), 4.9 (q, 1H), 4.2 (m, 1H), 4.0 (q, 2H), 3.9 (d, 1H), 2.5 (s, 1H), 2.0 (s, 2H), 1.2 (t, 1H). MS (FAB+) m/e 568 (M+H) ⁺ and MS (FAB-) m/e566 (M-H) ⁺ . Anal calcd for C32H25NO5: C, 67.72; H, 4.44; N, 2.47. Found: C, 67.68; H, 4.76; N, 2.35.

Example 171 5-fN-(2-flndol-3-vhethvπ-N-f4-phenoxybenzvπaminocarbonvnbe nzene-1.2.4- tricarboxylic acid

Example 17 A 5-rN-f2-πndol-3-y0ethylVN-(4-phenoxybenzvπamine A mixture of 3-(2-aminoethyl)indole (5.8 g, 0.036 mol) and p- phenoxybenzaldehyde (6.0 g, 0.03 mol) in ethanol (250 mL) containing anhydrous 10% palladium on carbon (1.2 g) was shaken for 7 hours without hydrogen and then for 24 hours under 4 atmospheres of hydrogen. The reaction mixture was then filtered, and the filtrate concentrated in vacuo. The residue obtained was purified, first by recrystallization from hexane and then by chromatography eluting with 1 :9:1 hexane-ethyl acetate-triethylamine to give the title compound as a white crystalline solid (5.5 g, 53%). m.p. 100-102 °C. MS (DCI/NH ₃ ) m/e 343 (M+H) ⁺ . Anal calcd for C23H22N2O: C, 80.67; H, 6.48; N, 8.18. Found: C, 80.67; H, 6.36; N, 8.13.

Example 171B 5-fN-f2-πndol-3-ynethvn-N-f4-phenoxybenzvnaminocarbonyl1ben zene-1.2.4- tricarboxylic acid A solution of the compound resulting from Example 171 A (0.7 g, 0.002 mol) in anhydrous THF (40 mL) was added dropwise under a nitrogen atmosphere to a stirred solution of 1 ,2,4,5-benzenetetracarboxylic dianhydride (0.51 g, 0.002 mol) in anhydrous THF (60 mL) containing N-methyl morpholine (0.21 g, 0.002 mol) cooled in a dry ice/acetone bath (-70 °C). After the addition was complete (approximately 2 hours), the cooling bath was removed, and the reactipn mixture was stirred at ambient temperature for 2 hours. The volatiles were removed under reduced pressure, and the yellow residue was dissolved in ethyl acetate. The solution was washed with 10% cold HCI and cold brine, filtered, dried and evaporated. The residue was stirred at ambient temperature with THF (60 mL) and saturated sodium carbonate solution (15 mL) for 1 hour, and then the THF was removed under reduced pressure. The residue was diluted with water, cooled in an ice bath, acidified with HCI and extracted with ethyl acetate. The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered and evaporated under reduced pressure. The residue was purified by column chromatography eluting with 20:1:5 chloroform-acetic acid-methanol to give the title compound (0.75 g, 68%) as a cream colored solid. MS (DCI/NH3) m/e 577 (M+H) ⁺ . ¹ H NMR (DMSO-d ₆ , 500 MHz) δ 10.82 (s, 1H), 10.7 (s, 1H), 8.58 (s, 1H), 8.5 (s, 1H), 7.6 (d, 1H), 7.4- 6.7 (m, 14H), 4.9 (s, 1H), 4.7 (s, 1H), 4.75 (s, 1H), 3.45 (s, 2H), 3.0 (s, 1H), 2.5 (s, 1H).

Example 172 4-r3-Benzyl-4-f4-phenoxyphenvπpyrazol-2-ynbenzene-1.2-dicar boxylic acid

Example 172A . 1 -(4-Phenoxy)phenyl-3-phenylprop-1 -yl alcohol

To a mixture of magnesium (292 mg, 12 mmol) and a small crystal of iodine in THF iβ mL) was added 1 mL of 2-phenylethyl bromide (2.04 g in 10 mL THF, 11 mmol). The mixture was gently heated with a heat gun until the reaction started (iodine color disappeared). The rest of phenylethyl bromide was added to the reaction over 20 minutes, and the reaction mixture was then heated at 50 °C for 1 hour, diluted with THF (15 mL), and cooled to 0 °C. To this mixture was added 4-phenoxybenzaldehyde (1.98 g, 10 mmol) over 10 minutes. After 1 hour at 0 °C, saturated ammonium chloride (5 mL) was added. The reaction mixture was diluted with ether (100 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 15% ethyl acetate in hexane to give the title compound (2.61 g, 86%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.31 (m, 6H), 7.20 (m, 3H), 7.12 (tt, 1H), 7.01 (m, 4H), 4.68 (m, 1H), 2.70 (m, 2H), 2.10 (m, 12H), 1.90 (d, 1H).

Example 172B 4-Phenoxyphenyl 2-phenylethyl ketone A mixture of the alcohol resulting from Example 172A (2.61 g, 8.58 mmol), pyridinium chlorochromate (2.78 g, 12.9 mmol) and activated molecular sieves (4 A) powder (3 g) in dichloromethane (50 mL) was stirred at 0 °C for 2 hours. The mixture was then diluted with ether to 100 mL, filtered through silica gel (50 g), and rinsed with ether. Evaporation of the filtrate in vacuo afforded the title compound in good purity (2.43 g, 94%). ¹ H NMR (300 MHz, CDCI3) δ 7.95 (dt, 2H), 7.39 (tt, 2H), 7.25 (m, 6H), 7.07 (dq, 2H), 6.99 (dt, 2H), 3.28 (t, 2H), 3.06 (t, 2H).

Example 172C Trimethylsilyl enol ether of 4-Phenoxyphenyl 2-phenylethyl ketone To a solution of the ketone resulting from Example 172B (302.3 mg, 1.0 mmol) in THF (5 mL) at -78 °C was added sodium bis(trimethylsilyl)amide (1.0 M solution in THF, 1.2 mL). After 30 minutes, trimethylsilyl chloride (0.127 mL, 1.5 mmol) was added to the reaction. The cooling bath was removed, and the reaction was warmed to room temperature over 30 minutes. The reaction mixture was then poured into saturated sodium bicarbonate solution (10 mL), and extracted with ether (80 mL). The organic layer was washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo to give the title compound in good purity. ¹ H NMR (300 MHz, CDCI3) δ 7.46 (dt, 2H), 7.30 (m, 6H), 7.20 (m, 1H), 7.11 (tt, 1H), 7.01 (dt, 2H), 6.94 (dt, 2H), 5.36 (t, 1H), 3.55 (d, 2H).

Example 172D

1.2-Dimethoxycarbonyl-4- -hvdroxy-3-phenyl-2-f4-phenoxybenzovπbenzene To a solution of the trimethylsilyl enol ether resulting from Example 172C (1.0 mmol) and 3,4-dimethoxycarbonylbenzaldehyde (222 mg, 1.0 mmol) in dichloromethane (6 mL) at -78 °C was added titanium tetrachloride (1.0 M in dichloromethane, 1.1 mL) over 10 minutes. After 5 hours, the reaction was poured into a mixture of ether (80 mL) and ice (10 g), and separated. The organic layer was washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 30% ethyl acetate in hexane to give the title compound (353 mg, 67%) as a 1:1 mixture of 2 diastereomers. ¹ H NMR (300 MHz, CDCI3) δ 7.80-6.80 (m, 17H), 5.17,4.97 (2 m's, 1H), 4.21 ,3.80 (2 d's, 1H), 3.97 (m, 1H), 3.93-3.86 (4 s's, 6H), 3.20-3.85 (4 d's, 2H).

Example 172E 1.2-Dimethoxycarbonyl-4-f1-oxo-3-phenyl-2-f4-phenoxybenzovnb enzene The procedure decribed in Example 172B was used to oxidize the alcohol resulting from Example 172D (251 mg, 0.48 mmol) to give the title compound (223 mg, 90%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.21 (d, 1 H), 7.98 (dd, 1H), 7.83 (dt, 2H), 7.67 (d, 1H), 7.39 (m, 2H), 7.20 (m, 6H), 7.04 (dq, 2H), 6.93 (dt, 2H), 5.41 (t, 1H), 3.91 (s, 3H), 3.88 (s, 3H), 3.43 (dq, 2H).

Example 172F 4-[3-Benzyl-4-f4-phenoxyphenyl)pyrazol-2-yl1benzene-1.2-dica rboxylic acid dimethyl ester A solution of the dione resulting from Example 172E (137 mg, 0.262 mmol), anhydrous hydrazine (39 mg, 1.31 mmol) and p-tolunesulfonic acid monohydrate (50 mg) in ethanol (5 mL) was stirred at room temperature for 60 hours. The residue after concentration of the filtrate was purified by column chromatography eluting with 30% ethyl acetate in hexane to give the title compound (122 mg, 90%). H NMR (300 MHz, CDCI3) δ 7.90 (t, 1 H), 7.68 (s, 2H), 7.42-7.24 (m, 6H), 7.17 (m, 4H), 7.02 (m, 4H), 4.09 (s, 2H), 3.89 (s, 3H), 3.83 (s, 3H).

Example 172G 4-f3-Benzyl-4-f4-phenoxyphenynpyrazol-2-vnbenzene-1.2-dicarb oxylic acid The compound resulting from Example 172F (115 mg) was stirred with saturated aqueous lithium hydroxide (0.5 mL) and methanol (3 mL) at 60 °C for 15 hours. The volatiles were removed by evaporation, and the residue was treated with aqueous 3 £J HCI (2 mL). The mixture was cooled to 0 °C for 10 minutes, and the precipitate was collected by suction filtration. The solid was then dissolved in boiling ethanol (2 mL) and treated with hot water (0.5 mL). The mixture was then gradually cooled to 0 °C, and the white crystalline powder was collected by suction filtration, rinsed with cold 70% ethanol in water, and air dried to give the title compound (103 mg, 93%). H NMR (500 MHz, DMSO-de) δ 7.90 (s, 1 H), 7.68 (t, 2H), 7.52 (d, 2H), 7.40 (t, 2H), 7.23 (t,

3H), 7.15 (q, 2H), 7.07 (d, 2H), 7.02 (m, 4H), 4.13 (s, 2H). MS (FAB+) m/e 491 (M+H)+

Example 173 4-f3-Phenyl-2-(4-phenoxybenzovfl-1 -propenyllbenzene-1.2-dicarboxylic acid

Example 173A 4-f3-Phenyl-2-(4-phenoxybenzovn-1 -propenyl1benzene-1.2-dicarboxylic acid dimethyl ester (\) and 4-f3 Phenyl-2-f4-phenoxybenzovπ-1-methanesulfonyloχypropyl benzene-1.2- dicarboxylic acid dimethyl ester (ii) To a 0 °C solution of the diastereomeric mixture of the hydroxyketones resulting from Example 172D (1.07 g, 2.04 mmol) in THF (30 mL) was added triethylamine (0.57 mL, 4.08 mmol), followed by methanesulfonyl chloride (0.189 mL, 2.45 mmol). The reaction was then stirred at room temperature for 2 Hours and treated with 1 ,8-diazabicyclo[5.4.0]undec-7-ene (0.46 mL, 3.06 mmol). The reaction was then stirred at room temperature for 18 hours. The reaction mixture was diluted with hexane (20 mL), and to it was added anhydrous 4 N HCI in 1 ,4-dioxane (1 mL). The mixture was then filtered through silica gel (30 g), and rinsed with ether. The residue after evaporation of the volatiles was purified by column chromatography eluting sequentially with 20% and 35% ethyl acetate in hexane to give 173i as the first fraction (382 mg, 37%), and 173ii as the second fraction (3:1 mixture of two diastereomers, 736 mg, 60%). 1H NMR for 173i (500 MHz, CDCI ₃ ) δ 7.82 (dt, 2H), 7.75 (d, 1H), 7.72 9d, 1 H), 7.55 (dd, 2H), 7.41 (t, 2H), 7.27 (t, 2H), 7.21 (m, 6H), 7.10 9d, 1 H), 7.02 (dt, 2H), 4.09 (s, 2H), 3.93 9s, 3H), 3.92 (s, 3H). ^" Η NMR for 173ii (the major diasteromer) (300 MHz, CDCI3) δ 7.89 (d, 1H), 7.78 (d, 1H), 7.67 (dt, 2H), 7.38 (m, 2H), 7.24-7.00 (m, 7H), 6.85 (m, 4H), 5.88 (d, 1 H), 4.21 (m, 1H), 3.96 (s, 3H), 3.95 (s, 3H), 2.86 (dd, 1H), 2.77 (s, 3H), 2.47 (dd, 1H).

Example 173B 4-f3-Phenyl-2-te-phenoxybenzovπ-1 -propenyl1benzene-1.2-dicarboxylic acid The enone resulting from Example 173A(i) (84 mg) was stirred with saturated aqueous lithium hydroxide (0.5 mL) and methanol (3 mL) at 60 °C for 15 hours. The reaction mixture was then acidified with aqueous 3 HCI (2 mL), diluted with ethyl acetate (50 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 95:5:0.5 ethyl acetate-methanol-formic acid to give the title compound (84 mg, 100%). H NMR (500 MHz, DMSO-d ₆ ) δ 7.88 (m, 3H), 7.66 (d, 1 H), 7.45 (m, 2H), 7.30-6.80 (m, 12H), 4.02 (s, 2H). MS (FAB-): m/e 477 (M-H)-.

Example 174 4-f3-Phenyl-2-f4-phenoxybenzovfl-1 -propenyllbenzene-1 ,2-dicarboxylic acid

Example 174A 4-r3-Phenyl-2-(4-phenoxybenzovfl-1 -propenyllbenzene-l ,2-dicarboxylic acid dimethyl ester To a solution of the methanesulfonate resulting from Example 173A (ii) (736 mg) in THF (15 mL) was added 1,8-diazabicyclo[5.4.0]undec-7-ene (0.5 mL). The reaction was stirred at 60 °C for 70 minutes and then diluted with hexane (10 mL) and filtered through silica gel (10 g) rinsing with ether. The residue after evaporation of the filtrate was carefully purified by column chromatography eluting with 15% ethyl acetate in hexane to give 173A(ii) as the first fraction (102 mg, 9.8% yield combined 2 steps), a fraction of mixture of compound 173A(ii) and 174A (65 mg, 6.2%), and 174A as the last fraction (364 mg, 35%). ^" <H NMR (300 MHz, CDCI ₃ ) δ 7.90 (dt, 2H), 7.65 (d, 1H), 7.59 (dd, 1H), 7.37 (m, 8H), 7.19 (tt, 1 H), 7.10 (s, 1H), 7.09 (dt, 2H), 7.02 (dt, 2H), 4.17 (s, 2H), 3.88 (s, 6H).

Example 174B 4-f3-Phenyl-2-(4-phenoxybenzovπ-1 -propenyl1benzene-1.2-dicarboxylic acid The procedure described in Example 173B was used to convert the enone resulting from Example 174A (42 mg) to the title compound (41 mg, 100%). 1 H NMR (500 MHz, DMSO-d6): δ 7.87 (dt, 1 H), 7.70 (m, 1 H), 7.56 (dd, 1H), 7.42 (m, 5H), 7.26 (m, 3H), 7.10 (m, 3H), 7.00 (m, 1H), 6.92 (m, 1H), 6.84 (m, 1H), 4.12 (s, 2H). MS (FAB ^* ): m/e 477 (M-H)-.

Example 175 trans-4-f3-Hydroxy-2-benzyl-3-f4-phenoxyphenvπ-1-propenvπb enzene-1.2- dicarboxylic acid

Example 175A 4-[3-Hvdroxy-2-benzyl-3-f4-phenoxyphenvπ-1-propenvnbenzene- 1.2- dicarboxylic acid dimethyl ester

To a solution of the enone resulting from Example 173A(i) (62.3 mg, 0.123 mmol) in methanol (3 mL) was added cerium trichloride (36.5 mg, 0.148 mmol) followed by sodium borohydride (6 mg, 0.15 mmol). After 5 minutes, saturated ammonium chloride (1 mL) was added to the reaction mixture. The reaction mixture was diluted with ether (50 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo to give the title compound (63 mg) in good purity. H NMR (300 MHz, CDCI3) δ 7.71 (d, 1 H), 7.63 (d, 1H), 7.47 (dd, 1H), 7.40-7.20 (m, 7H), 7.12 (m, 4H), 7.00 (m, 4H), 5.18 (m, 1 H), 3.90 (s, 3H), 3.88 (s, 3H), 3.82 (d, 1H), 3.23 (d, 1H), 1.92 (d, 1H).

Example 175B trans-4-[3-Hydroxy-2-benzyl-3-(4-phenoxyphenylV1-propenvnben zene-1.P- dicarboxylic acid The procedure described in Example 173B was used to convert the enone resulting from Example 175A (58 mg) to the title compound (56 mg, 99%). H NMR (300 MHz, CDCI ₃ ) δ 13.5 (very br s, 2H), 7.66 (d, 1H), 7.61 (s, 1H), 7.45 (d, 1H), 7.36 (m ,4H), 7.23 (t, 2H), 7.10 (m, 4H), 6.95 (t, 4H), 5.73 (s, 1H), 5.08 (s, 1H), 3.75 (d, 1 H), 3.17 (d, 1 H). MS (FAB ") m/e 479 (M-H)".

Example 176 cis-4-f3-Hydroxy-2-benzyl-3-f4-phenoxyphenvπ-1-propenynbenz ene-1.2- dicarboxylic acid

Example 176A cis-4-f3-Hydroxy-2-benzyl-3-(4-phenoxyphenvO-1-propenvπbenz ene-1.2- dicarboxylic acid dimethyl ester The procedure described in Example 175A was used to convert the enone resulting from Example 174A (115 mg) to the title compound (115 mg). 1H NMR (300 MHz, CDCI3) δ 7.65 (d, 1H), 7.44 (dd, 1H), 7.38-7.20 (m, 10H, 7.13 (m, 2H), 6.99 (m, 3H), 6.96 (d, 1H), 5.16 (m, 1H), 3.89 (s, 6H), 3.86 (d, 1H), 3.38 (d, 1H), 1.93 (d, 1H).

Example 176B cis-4- 3-Hydroxy-2-benzyl-3-f4-phenoxyphenvπ-1-propenvnbenzene-1.2 - dicarboxylic acid

The procedure described in Example 173B was used to convert the enone resulting from Example 176A (48 mg) to the title compound (45 mg, 94%). 1H NMR (500 MHz, DMSO-d ₆ ) δ 7.40-7.20 (m, 12H), 7.13 (m, 1 H), 6.92 (m, 4H), 5.71 (s, 1H), 5. 09 (s, 1H), 3.75 (d, 1H), 3.40 (d, 1H). MS (FAB") m/e 479 (M-H)-.

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Example 177 trans-4-r2-Benzyl-3- 4-phenoxyphenyl)-1-propenyl benzene-1.2-dicarboxylic acid

Example 177A trans-4-r2-Benzyl-3-f4-phenoxyphenyl)-1-propenynbenzene-1.2- dlcarboxylic acid dimethyl ester The compound resulting from Example 175A (180 mg, 0.352 mmol) was stirred with triethylsilane (0.4 mL) and trifluoroacetic acid (2 mL) at 70 °C for 8 hours. The reaction mixture was then concentrated in vacuo, and the residue was purified by column chromatography eluting with 15% ethyl acetate in hexane to give the title compound (161 mg, 93%). ¹ H NMR (300 MHz, CDCI3) δ 7.69 (d, 1H), 7.57 (d, 1H), 7.43 (dd, 1H ), 7.30 (m, 5H), 7.12 (m, 5H), 7.01 (dq, 2H), 6.95 (dt, 2H), 6.51 (s, 1H), 3.90 (s, 3H), 3.89 (s, 3H), 3.55 (s, 2H), 3.38 (s, 2H).

Example 177B trans-4-r2-Benzyl-3-(4-phenoxyphenylH-propenyl1benzene-1.2-d icarboxylic acjd The procedure described in Example 173B was used to convert the olefin resulting from Example 177A (61 mg) to the title compound (60 mg, 100%). 1H NMR (500 MHz, DMSO-d ₆ ) δ 7.89 (d, 1H), 7.83 (s, 1H), 7.44 (dd, 1H), 7.38 (t, 2H), 7.30 (t, 2H), 7.21 (m, 3H), 7.14 (m, 3H), 6.97 (m, 4H), 6.63 (s, 1H), 3.52 (s, 2H), 3.35 (s, 2H). MS (FAB-): m/e 463 (M-H)-.

Example 178 cis-4-f2-Benzyl-3-f4-phenoxyphenyπ-1 -propenyllbenzene-1.2-dicarboxylic aniH

Example 178A cis-4-f2-Benzyl-3-f4-phenoxyphenvπ-1-propenvnbenzene-1.2-di carboxylic acid dimethyl ester The procedure described in Example 177A was used to convert the compound resulting from Example 176A (47 mg) to the title compound (43 mg, 95%). 1H NMR (300 MHz, CDCI ₃ ) δ 7.70 (d, 1H), 7.43 (dd, 1H), 7.37-7.17 (m, 6H), 7.10 (m, 4H), 7.01 (m, 2H), 6.93 (m, 3H), 6.64 (s, 1H), 3.91 (s, 6H), 3.61 (s, 2H), 3.35 (s, 2H).

Example 178B cis-4-f2-Benzyl-3-(4-phenoxyphenyπ-1 -propenyllbenzene-l .2-dicarboxylic acid The procedure described in Example 173B was used to convert the olefin resulting from Example 178A (40 mg) to the title compound (37 mg, 94%). ¹ H NMR (500 MHz, DMSO-d ₆ ) δ 7.75 (d, 1H), 7.54 (s, 1H), 7.38-7.10 (m, 11H), 6.96 (m, 4H), 6.67 (s, 1H), 3.59 (s, 2H), 3.37 (s, 2H). MS (FAB-) m/e 463 (M-H)-.

Example 179

4-f2-Benzyl-3-(4-phenoxyphenyl)-1 -propyHbenzene-l .2-dicarboxylic acid

Example 179A 4-[2-Benzyl-3-f4-phenoxyphenvn-1 -propyl]benzene-1 ,2-dicarboxylic acid dimethyl ester

A mixture of the olefin resulting from Example 177A (107 mg) and 10% palladium on carbon (100 mg) in ethanol (2 mL) and ethyl acetate (2 mL) was stirred under a hydrogen balloon for 5.5 hours. The reaction mixture was then diluted with ether (20 mL), filtered through silica gel (5 g) and rinsed with ether. Concentration of the filtrate afforded the title compound (105 mg, 99%) in good purity. 1H NMR (300 MHz, CDCI3) δ 7.67 (d, 1H), 7.41 (d, 1H), 7.35-7.14 (m, 5H), 7.11-7.02 (m, 6H), 6.98 (m, 2H), 6.92 (dt, 2H), 3.91 9s, 3H), 3.89 (s, 3H), 2.64-2.44 (m, 6H), 2.30 (m, 1H).

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Example 179B 4-f2-Benzyl-3-f4-phenoxyphenvπ-1 -propyllbenzene-1.2-dicarboxylic acid The procedure described in Example 173B was used to convert the compound resulting from Example 179A (83 mg) to the title compound (54 mg, 69%). ¹ H NMR (500 MHz, DMSO-d ₆ ) δ 7.92 (br s, 1H), 7.76 (br s, 1H), 7.37 (m, 2H), 7.28 (m, 2H), 7.14 (m.m 7H), 6.97 (dt, 2H), 6.90 (dt, 2H), 2.58 (d, 2H), 2.50 (m, 4H), 2.14 (m, 1H). MS (FAB-) m/e 465 (M-H)-.

Example 180

6-Vinyl-3-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonyl]benz ene-1.4- dicarboxylic acid

Example 180A 3.6-Diftrifluoromethanesulfonyloxy]benzene-1.4-dicarboxylic acid diethyl ester To a 0 °C solution of diethyl 2,5-dihydroxyterephthalate (4.24 g, 16.7 mmol) and pyridine (8 mL) in dichloromethane (30 mL) was slowly added trifluoromethanesulfonic anhydride (5.9 mL, 35 mmol). The reaction was allowed to warm to ambient temperature over 2 hours, and another portion of trifluoromethanesulfonic anhydride (1 mL, 5.7 mmol) was added. After another 1 hour, the reaction was diluted with ethyl acetate (200 mL), washed with aqueous 1 £J HCI (50 mL), saturated copper (II) sulfate (50 mL), water (50 mL), and brine (50 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was recrystallized from ethyl acetate, and the mother liquid was concentrated and purified by column chromatography eluting with 10%.ethyl acetate in hexane to give the title compound (8.05 g combined yield, 93%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.98 (s, 2H), 4.51 (q, 4H), 1.44 (t, 6H).

Example 180B 2-Trifluoromethanesulfonyloxy-5-vinylbenzene-1.4-dicarboxyli c acid diethyl ester A solution of the bistriflate resulting from Example 180A (5.19 g, 10.0 mmol), vinyltributyltin (3.17 g, 10.0 mmol), bis(triphenylphosρhine)palladium dichloride (210 mg, 0.3 mmol), and anhydrouse lithium chloride (1.3 g, 30 mmol) in anhydrous 1,4-dioxane (30 mL) was degassed by bubbling nitrogen through the solution for 5 minutes. The mixture was then heated at 85 °C for 6 hours. After the reaction mixture was cooled to room temperature, it was filtered through silica gel (30 g), and the filtrate was diluted with ether to 100 mL. To the filtrate was added water (0.5 mL), followed by the dropwise addition of DBU (3 mL) with good stirring. The mixture was immediately filtered through silica gel (50 g), rinsed with ether several times, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 5% followed by 10% ether in hexane to give the title compound (3.73 g, 94%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.24 (s, 1H), 7.78 (s, 1H), 7.44 (dd, 1H), 5.78 (dd, 1H), 5.50 (dd, 1H), 4.48 (q, 2H), 4.41 (q, 2H), 1.44 (t, 3H), 1.42 (t, 3H).

Example 180C 2.5-Diethoxycarbonyl-4-trifluoromethanesulfonyloxybenzoic acid

A mixture of the olefin resulting from Example 180B (711 mg, 1.79 mmol), sodium periodate (1.96 g, 9.0 mmol) and ruthenium trichloride monohydrate (11 mg, 0.05 mmol) in a mixed solvent system of carbon tetrachloride (4 mL), acetonitrile (4 mL) and water 96 mL) was well stirred at 60 °C for 4 hours. After allowing to cool to ambient temperature, the reaction mixture was diluted with ethyl acetate (100 mL), washed with 0.1 N HCI, water and brine, dried over anhydrous magnesium sulfate, filtered through silica gel (20 g), washed with 5% methanol in ethyl acetate, and concentrated in vacuo to give the title compound which was used without further purification. ¹ H NMR (300 MHz, CDCI3) δ 8.58 9s, 1 H), 7.62 (s, 1 H), 4.47 (m, 4H), 1.44 (t, 3H), 1.38 (t, 3H).

Example 180D 1.4-Diethoxycarbonyl-2-trifluoromethanesulfonyloxy-5-[N-benz yl-N-4- henoxybenyl)aminocarbonyl ^' |benzene To a suspension of the acid resulting from Example 180C in dichloromethane (5 mL) was added oxalyl chloride (2.0 M in dichloromethane, 1. 8 mL), followed by the addition of a tiny drop of N,N-dimethylformamide. After the mixture was stirred 2 hours at room temperature, the solvent was evaporated, and the residue was further dried under high vacuum for 10 minutes. The acid chloride was redissolved in dichloromethane (5 mL), and N- benzyl-N-(4-phenoxy)benzylamine (625 mg, 2.16 mmol) was added to the reaction mixture. After 30 minutes, the reaction mixture was filtered through silica gel (10 g), and then rinsed with ethyl acetate. The filtrate was concentrated in vacuo, and the residue was purified by column chromatography eluting with 20% ethyl acetate in hexane to give the title compound (1.01 g, 82% for 2 steps). H NMR (300 MHz, CDCI ₃ ) δ 7.98,7.96 (2 s's, 1H), 7.90,7.88 (2 s's, 1H), 7.42-7.28 (m, 8H), 7.15-7.95 (m, 6H), 4.79,4.76 (2 s's, 2H), 4.47-4.30 (4 q's, 4H), 4.24,4.20 (2 s's, 2H), 1.43-1.25 (4 t's, 6H).

Example 180E 1.4-Diethoxycarbonyl-6-vinyI-3-| ^' N-benzyl-N-4- henoxybenyπaminocarbonyl]benzene The method described in Example 180B was used to convert the olefin resulting from Example 180D (945 mg, 1.38 mmol) to the title compound (724 mg, 94%). 1H NMR (300 MHz, CDCI3) δ 8.21,8.18 (2 s's, 1H), 7.87,7.865 (2s's, 1H), 7.50-7.28 (m, 9H), 7.16-7.96 (m, 6H), 5.79,5.73 (2 dd's, 1H), 5.48,5.44 (2 dd's, 1H), 4.77,4.72 (2 br s's, 2H), 4.40-4.30 (4 q's, 4H), 4.26,4.22 (2 s's, 2H), 1.40-1.23 (4 t's, 6H).

Example 180F 6-Vinyl-3-rN-Benzyl-N-f4-phenoxybenzyπaminocarbonyl1benzene - _r 4- dicarboxylic acid The procedure described in Example 173B was used to convert the compound resulting from Example 180E (31 mg) to the title compound (7.5 mg, 28%). 1H NMR (300 MHz, CD ₃ OD) δ 8.30,8.29 (2 s's, 1 H), 7.76,7.74 (2s's, 1H), 7.57-7.24 (m, 9H), 7.16-7.93 (m, 6H), 5.82 (d, 1H), 5.42 (dd, 1H), 4.85-4.76 (2H), 4.30,4.28 (2 s's, 2H). MS (FAB+) m/e 508 (M+H)+.

Example 181

6-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonvn-3-hvdroxy-1- oxo-1.3- dihvdroisobenzofuran-5-carboxylic acid

Example 181 A 3-fN-Benzyl-N-(4-phenoxybenzyl)aminocarbonvn-6-formyl-benzen e-1.4- dicarboxylic acid diethyl ester A mixture of the olefin resulting from Example 180E (714 mg, 1.27 mmol), sodium periodate (0.66 M in water, 5.8 mL, 3.81 mL), and osmium tetraoxide (0.08 M in tert-butanol, 0.32 mL, 0.025 mmol) in 1 ,4-dioxane (20 mL) was stirred at room temperature for 1 hour. The reaction mixture was diluted with ether (100 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 30% ethyl acetate -in hexane to give the title compound (691 mg, 96%). ¹ H NMR (300 MHz, CDCI3) δ 10.62,10.61 (2 s's. 1 H), 8.56,8.55 (2 s's, 1 H), 7.92 (s, 1 H), 7.43-7.28 (m, 8H), 7.15-6.93 (m, 6H), 4,78 (br s, 2H), 4.47-4.33 (4 q's, 4H), 4.23,4.18 (2 s's, 2H), 1.43-1.32 (4 t's, 6H).

Example 181B 6-fN-Benzyl-N-f4-phenoxybenzvnam?nocarbonyl1-3-hvdroxy-1-oxo -1.3- dihvdrolsobenzofuran-5-carboxylic acid The method described in Example 173B was used to convert the adehyde resulting from Example 181 A (721 mg, 1.28 mmol) to the title compound (619 mg, 95%). ¹ H NMR (500 MHz, DMSO-de) δ 8.31 ,8.30 (2 s's, 1H), 7.77,7.76 (2 s's, 1H), 7.38-7.22 (m, 8H), 7.15-6.83 (m, 6H), 6.64 (br s, 1H), 4.20 (m, 4H). MS (FAB+) m/e 510 (M+H)+.

Example 182

6-fN-Benzyl-N-f4-phenoxybenzyπaminocarbonvn-3-methoxy-1- oxo-1.3- dihydroisobenzofuran-5-carboxylic acid A mixture of the hemiacetal resulting from Example 181B (331 mg, 0.65 mmol), anhydrous 4.0 N HCI in dioxane (0.5 mL), anhydrous 4A molecular sieves (1 g) in methanol (3 mL) was stirred at 60 °C for 15 hours. The reaction mixture was diluted with ethyl acetate to 20 mL, filtered through celite, rinsed with ethyl acetate, and concentrated in vacuo. The residue was then purified by column chromatography eluting sequentially with 100% ethyl acetate, followed by 2% and 5% methanol in ethyl acetate to give the title compound (71 mg, 21%). 1H NMR (500 MHz, DMSO-d ₆ ) δ 8.18,8.16 (2 s's, 1H), 7.81 ,7.78 (2 s's, 1H), 7.42-7.24 (m, 7H), 7.17 (m, 3H),7.04-6.85 (m, 4H), 6.60,6.58 (2 s's, 1H), 4.25,4.22 (2 br s's, 4H), 3.57 (s, 3H). MS (FAB+) m/e 524 (M+H)+.

Example 183 6-[N-Benzyl-N-f4-phenoxybenzyl)aminocarbonylmethvπ-3-methox y-1-oxo-l .3- dihydroisobenzofuran-5-carboxylic acid

Example 183A 2-Trifluoromethanesulfonyloxy-5-allyl-benzene-1.4-dicarboxyl ic acid diethyl ester

The method used in Example 180B was used to convert the bistriflate resulting from Example 180A (1.04 g, 2.0 mmol) to the title compound (748 mg, 91%). 1H NMR (300 MHz, CDCI ₃ ) δ 7.96 (s, 1H), 7.77 (s, 1H), 5.96 (m, 1H),

5.52 (dq, 1H), 5.05 9dq, 1 H), 4.46 (q, 2H), 4.39 (q, 2H), 3.80 (dt, 2H), 1.43 (t, 3H), 1.40 (t, 3H).

Example 183B 2-Trifluoromethanesulfonyloxy-5-carboxymethyl-benzene-1.4-di carboxylic acid diethyl ester The method described in Example 180C was used to convert the olefin resulting from Example 183A (974 mg, 1.81 mmol) to the title compound (560 mg, 72%). 1H NMR (300 MHz, DMSO-d ₆ ) δ 12.51 (s, 1H), 8.10 (s, 1 H), 7.89 (s, 1H), 4.39 (q, 2H), 4.31 (q, 2H), 4.07 (s, 2H), 1.35 (t, 3H), 1.30 (t, 3H).

Example 183C 2-Trifluoromethanesulfonyloxy-5-fN-benzyl-N-f4- phenoxybenzvnaminocarbonylmethyl]benzene-1.4-dicarboxylic acid diethyl ester

The method described in Example 180D was used to convert the acid resulting from Example 183B (547 mg, 1.28 mmol) to the title compound (815 mg, 91%). 1H NMR (300 MHz, CDCI3) δ 7.90 (d, 1H), 7.89 (s, 1H), 7.43-7.22 (m, 10H), 7.13 (m, 1H), 7.06-6.94 (m, 3H), 4.63,4.62,4.60,4.58 (4 s's, 4H), 4.45 (2 q's, 2H), 4.35 (2 q's, 2H), 4.25, 4.22 (2 s's, 2H), 1.42-1.32 (4 t's, 6H).

Example 183D 5-Vinyl-2-[N-benzyl-N-f4-phenoxybenzvπaminocarbonylmethvπb enzene-1.4- dicarboxylic acid diethyl ester The method described in Example 180B was used to convert the compound resulting from Example 183C (808 mg, 1.16 mmol) to the title compound (605 mg, 90%). ¹ H NMR (300 MHz, CDCI3) δ 8.18 (s, 1H), 7.73 (d, 1H), 7.47-7.23 (m, 11H), 7.11 (m, 1H), 7.05-6.93 (m, 3H), 6.75 (d, 1 H), 5.39 (D, 1H), 4.62,4.60,4.57 (3 S'S, 2H), 4.41-4.38 (4 q'S, 4H), 4.20,4.17 (2 s's, 2H), 1.41-1.32 (4 t's, 6H).

Example 183E 2-Formyl-5-rN-benzyl-N-(4-phenoxybenzvπaminocarbonylmethyll benzene-1 _l 4- dicarboxylic acid diethyl ester The method described in Example 181 A was used to convert the compound resulting from Example 183D (344 mg, 0.595 mmol) to the title compound (265 mg, 76%). 1H NMR (300 MHz, CDCI ₃ ) δ 1H NMR (300 MHz, CDCI3): δ 10.62,10.61 (2 s's. 1H), 8.54,8.53 (2 s's, 1H), 7.83,7.81 (2 s's, 1H), 7.43-7.23 (m, 10H), 7.12 (m, 1H), 7.07-6.93 (m, 3H), 4.64,4.63,4.61 ,4.59 (4 s's, 4H), 4.45 (2 q's, 2H), 4.35 (2 q's, 2H), 4.30, 4.27 (2 s's, 2H), 1.45-1.35 (4 t's, 6H).

Example 183F

6-rN-Benzyl-N-f4-phenoxybenzvπaminocarbonylmethyll-3-met hoxy-1-oxo-1.3- dihydroisobenzofuran-5-carboxylic acid The aldehyde resulting from Example 183E (30 mg) was stirred with saturated aqueous lithium hydroxide (0.5 mL) and methanol (3 mL) at 45 °C for 15 hours. The reaction mixture was then acidified with aqueous 3 N HCI (2 mL), stirred at room temperature for 30 minutes, diluted with ethyl acetate (50 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 5% methanol in ethyl acetate to give the title compound (25 mg, 74%). ¹ H NMR (500 MHz, DMSO-d ₆ ) δ 8.11 ,8.10 (2 s's, 1H), 7.86,7.83 (2 s's, 1H), 7.43-7.20 (m, 10H), 7.15 (m, 1H), 7.04-6.90 (m, 3H), 6.60 (s, 1H), 4.65,4.62 (2 s's, 2H), 4.46,4.43,4.41,4.38 (4 s's, 4H), 3.58 (s, 3H). MS (FAB ⁺ ) m/e 538 (M+H)+.

Example 184

6-[N-Benzyl-N-(4-phenoxybenzyπaminocarbonylmethyl]-1 -oxo-1.3- dihvdroisobenzofuran-5-carboxylic acid

Example 184

6-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonylmethvn-1 -oxo-1.3- dihvdroisobenzofuran-5-carboxylic acid ethyl ester To 0 °C a solution of the aldehyde resulting from Example 183E (232 mg, 0.40 mmol) in tetrahydrofuran (4 mL) and methanol (1 mL) was added sodium borohydride (38 mg, 1.0 mmol). After 20 minutes, the reaction mixture was diluted with ether (100 mL), washed with 0.1 N. HCI, water, brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 30% ethyl acetate in hexane to give the title compound (151 mg, 70%). ¹ H NMR (300 MHz, CDCI ₃ ): δ 8.16(s, 1H), 7.85,7.81 (2 s's, 1H), 7.43-7.22 (m, 10H), 7.15 (m, 1 H), 7.08-6.92 (m, 3H), 5.35 (s, 2H), 4.66,4.64,4.62,4.60 (4 s's, 4H), 4.36,4.35 (2 q's, 2H), 4.30,4.27 (2 s's, 2H), 1.35-1.34 (2 t's, 3H).

Example 184B e-fN-Benzyl-N-^-phenoxybenzvπaminocarbonylmethvπ-l-oxo-l _r a- dihvdroisobenzofuran-5-carboxylic acid The lactone resulting from Example 184A (141 mg, 0.26 mmol) was stirred with saturated aqueous lithium hydroxide (0.5 mL) and methanol (3 mL) at 45 °C for 15 hours. The reaction mixture was then acidified with aqueous 3 H HCI (2 mL), diluted with ethyl acetate (50 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo. The residue was then stirred with p-toluensulfonic acid (10 mg), anhydrous molecular sieves (4A, 1 g) in toluene (5 mL) at 80 °C for 60 hours. The reaction mixture was filtered through celite, rinsed with ethyl acetate, and concentrated in vacuo. The residue was then purified by column chromatography eluting with 96:3:0.5 ethyl acetate-methanol-acetic acid to give the title compound (77 mg, 58%). H NMR (500 MHz, CDCL3): δ 8.02,8.00 (2 s's, 1H), 7.55,7.46 (2 s's, 1H), 7.38-7.30 (m, 10H), 7.18-6.97 (m, 2H), 6.90-6.83

(m, 2H), 5.24,5.23 (2 s's, 2H), 4.63,4.60,4.58,4.57 (4 s's, 4H), 4.17,4.10 (2 s's, 2H). MS (FAB -): m/e 506 (M-H).

Example 185 5-Hvdroxymethyl-2-rN-benzyl-N-(4- phenoxybenzvhaminocarbonylmethyπbenzene-1.4-dicarboxylic acid disodium salt To a solution the lactone resulting from Example 184B (42.1 mg, 0.083 mmol) in THF (3 mL) was added aqueous 2.00 M sodium hydroxide (0.249 mL). After.1 hour, the solvent was evaporated in vacuo to give the title compound (51.2 mg, 100%). 1 H NMR (500 MHz, DMSO-d ₆ ) δ 8.08 (q, 1 H), 7.68, 7.66, 7.61, 7.57 (4 s's, 1H), 7.43-7.30 (m, 11H), 7.01-6.89 (m, 3H), 5.34 (d, 2H), 4.63- 4.26 (many singlets, 6H), 3.67 (dd, 2H).

Example 186

6-fN-Benzyl-N-f4-phenoxybenzvflaminocarbonylamino1-3-meth oxy-1-oxo-1.3- dihvdroisobenzofuran-5-carboxylic acid

Example 186A Dimethyl 2-amino-5-bromoterephthalate

To a -40 °C solution of dimethyl aminoterephthalate (4.39 g, 21 mmol) and pyridine (2.0 mL, 23 mmol) in dichloromethane (40 mL) was added slowly bromine (1.0 M in dichloromethane, 22 mL) over 15 minutes. After 1 hour at -45 °C, the reaction was diluted with a mixture of ether and hexane (1:1, 40 mL), and was filtered through silica gel (50 g), rinsed with ether and dichloromethane (1:1), and concentrated in vacuo. The residue was then crystallized from ethyl acetate to give the title compound as yellowish green needles (4.23 g), and the mother liquid was concentrated in vacuo, and then purified by column chromatography eluting with 15% ethyl acetate in hexane to give more title compound (1.27 g, combined yield, 91%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.10 (s, 1H)), 7.06 (s, 1H), 5.80 (br s, 2H), 3.92 (s, 3H), 3.89 (s, 3H).

Example 186B Dimethyl 2-amino-5-vinylterephthalate The method described in Example 180B was used to convert the bromide resulting from Example 185A (4.43 g, 15.4 mmol) to the title compound (2.74 g, 76 %). 1H NMR (300 MHz, CDCI ₃ ) δ 8.11 (s, 1H), 7.21 (dd, 1H), 7.14 (s, 1H), 5.78 ( brs, 2H), 5.55 (dd, 1H), 5.19 (dd, 1H), 3.91 (s, 3H), 3.89 (s, 3H).

Example 186C Dimethyl 2-fN-Benzyl-N-f4-phenoxybenzvhaminocarbonylamino]-5- vinylterephthalate

A solution of the compopund resulting from Example 186B (2.72 g, 11.6 mmol) and triphosgene (1.21 g, 4.06 mmol) in THF (15 mL) was heated at 60 °C for 16 hours. After it was cooled to room temperature, N-benzyl-N-(4- phenoxy)benzylamine (4.36 g, 15.1 mmol) and triethylamine (4.9 mL, 35 mmol) were added sequentially. The reaction was then heated at 505 °C for 3 hours. The reaction mixture was filtered through silica gel (50 g), rinsed with ether, and concentrated in vacuo. The residue was purified by column chromatography eluting with 20% ethyl acetate in hexane to give the title compound (4.78 g, 75%). ¹ H NMR (300 MHz, CDCI3) δ 10.80 (s, 1H), 9.14 (s, 1H), 8.19 (s, 1H), 7.38-7.22 (m, 10H), 7.10 (tt, 1H), 6.98 (m, 4H), 5. 63 (dd, 1H), 5.31 (dd, 1H), 4.65 (br s, 2H), 4.62 (br s, 2H), 3.92 (s, 3H), 3.84 (s, 3H).

Example 186D Dimethyl 2-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonylamino1-5- formylterephthalate

The method described in example 181 A was used to convert the compound resulting from Example 186C (2.61 g, 4.73 mmol) to the title compound (2.13 g, 81%). ¹ H NMR (300 MHz, CDCI3) δ 11.22 (s, 1H), 10.42 (s, 1H), 9.26 (s, 1H), 8.63 (s, 1H), 7.38-7.22 (m, 9H), 7.10 (tt, 1H), 6.98 (m, 4H), 4.67 (s, 2H), 4.63 (s, 2H), 3.98 (s, 3H), 3.86 (s, 3H).

Example 186E 6-rN-Benzyl-N-(4-phenoxybenzvπaminocarbonylamino -3-methoxy-1-oxo-1.3- dihydroisobenzofuran-5-carboxylic acid The method described in Example 183F was used to convert the aldehyde resulted from Example 186D (629 mg, 1.14 mmol) to the title compound (517 mg, 84%). ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 8.90 (s, 1H), 8.17 (s, 1H), 7.41-7.24 (m, 9H), 7.12 (tt, 1H), 6.98 (m, 4H), 6.50 (s, 1H), 4.61 (br s, 2H), 4.58 (br s, 2H). MS (FAB+) m/e 539 (M+H)+.

Example 187

5-fN-Benzyl-N-(4-phenoxybenzyflaminocarbonylamino)benzene -1.2.4- tricarboxylic acid

Example 187A 5-rN-Benzyl-N-(4-phenoxybenzyflaminocarbonylamino ^' lbenzene-1.2.4- tricarboxylic acid 1 ,4-dimethyl ester The method described in Example 180C was used to convert the compound resulting from Example 186C (2.17 g, 3.94 mmol) to the title compound (973 mg, 43%). ¹ H NMR (300 MHz, CDCI3) δ 11.23 (s, 1H), 8.99 (s, 1 H), 8.69 (s, 1 H), 7.39-7.22 (m, 9H), 7.11 (tt, 1 H), 6.99 (m, 4H),.4.65 (br s, 2H), 4.61 (br s, 2H), 3.94 (s, 3H), 3.87 (s, 3H).

Example 187B 5- ^r N-Benzyl-N-f4-phenoxybenzyl)aminocarbonylamino1benzene -1.2.4- tricarboxylic acid

The method described in Example 173B was used to convert the compound resulting from Example 187A (132 mg, 0.23 mmol) to the title compound (91 mg, 72%). ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 8.89 (br s, 1H), 8.43 (br s, 1H), 7.42-7.26 (m, 9H), 7.14 (tt, 1H), 6.98 (m, 4H), 4.63 (br s, 2H), 4.60 (br s, 2H). MS (FAB -): m/e 539 (M-H).

Example 188 5-fN-Benzyl-N-^4-f4-methoxyphenoxy)benzyl)aminocarbonyllbenz ene-1.2.4- tricarboxylic acid To a mixture of 774 mg (19.4 mmol) of a 60% emulsion of sodium hydride in mineral oil in 3 mL of dry dimethylformamide (DMF) cooled to 0 °C, was slowly added a solution of 2.00 g (16.1 mmol) of 4-methoxyphenol in 3 mL of dry DMF. The mixture was allowed to warm to ambient temperature and was then treated with a solution of 4-fluorobenzaldehyde in 3 mL of dry DMF. After 18 hours, 30 mL of water was added, and the mixture was extracted with ethyl acetate. The combined extracts were washed repeatedly with water, dried (MgS04), and all volatiles were removed under reduced pressure affording 3.7 g (100%) of imine as a yellow oil. This crude product was dissolved in 20 mL of ethanol, to which was added 1.72 g (16.1 mmol) of benzylamine. The solution was stirred for 6 hours under a nitrogen atmosphere and was then treated with 0.61 g (16 mmol) of sodium borohydride. After 20 hours all volatiles were removed under reduced pressure, water was added and the mixture was extracted with ethyl acetate. All volatiles were removed under reduced pressure and concentrated hydrochloric acid was added. The resulting solid was crystallized from ethanol, affording 2.76 g (54%) of the amine hydrochloride as a white solid.

A mixture of 1.06 g (3.32 mmol) of the amine and 0.724 g (3.32 mmol) of 1 ,2,4,5-benzenetetracarboxylic dianhydride in 600 mL of dry tetrahydrofuran (THF) was cooled to -50 °C under a nitrogen atmosphere. To this was added dropwise 1.01 g (9.97 mmol) of triethylamine in 50 mL of dry THF. The reaction mixture was allowed to slowly warm to ambient temperature. After 20 hours all volatiles were removed under reduced pressure. The product was dissolved in 40 mL of ethyl acetate, and the solution was extracted with dilute hydrochloric acid. All volatiles were removed under reduced pressure, and the resulting product was dissolved in 100 mL of THF. To this was added 20 mL of saturated sodium carbonate, and the mixture was stirred vigorously for 6 hours. The mixture was acidified with concentrated HCI, and the solution was extracted with ethyl acetate. The extracts were dried (MgS04) and all volatiles were removed under reduced pressure, affording an oil. Purification by flash column

chromatography on silica gel eluting with 180:1:1 ethyl acetate-formic acid- water afforded 110 mg (6%) of the title compound as a foam. ¹ H NMR (300 MHz, DMSO-de) δ 3.75 (s, 3H), 4.1-4.2 (m, 2H), 4.4-4.5 (m, 2H), 6.8-7.4 (m, 15H). MS (FAB)" m/e 554 (M-H)".

Example 189 5-[N-Benzyl-N-f3-f4-methylphenoxy^benzvπaminocarbonvnbenzen e-1.2.4- tricarboxylic acid The corresponding amine was prepared from 3-(4- methylphenoxy)benzaldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 2.52 (s, 3H), 4.20 (d, J=8 Hz, 2H), 4.3-4.8 (m, 2H), 6.8-7.0 (m, 5H), 7.1-7.6 (m, 10H). MS (FAB)" m/e 538 (M-H)-.

Example 190 δ-fN-Benzyl-N-^-^-tert-butylphenoxy^benzvπaminocarbonvπbe nzene-l ^^- tricarboxylic acid The corresponding amine was prepared from 3-(4-t- butylphenoxy)benzaldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz,

DMSO-de) δ 1,27 (s, 9H), 4.0-4.2 (m, 4H), 6.8-7.0 (m, 5H), 7.1-7.5 (m, 10H). MS (FAB)" m/e 580 (M-H)".

E ample 191 5-[N-Benzyl-N-f4-fallyloxybenzvπaminocarbonyπbenzene-1.2.4 -tricarboxylic acid The corresponding amine was prepared from 4-allyloxybenzaldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 4.1-4.2 (m, 2H), 4.5-4.6 (m, 2H), 5.2-5.3 (m, 1 H), 5.3-5.5 (m, 1 H), 5.9-6.1 (m, 1 H), 6.8-6.9 (m, 2H), 7.0-7.2 (m, 2H), 7.2-7.5 (m, 6H), 7.6-7.7 (m, 1H). MS (FAB)" m/e 488 (M- H)-.

Example 192 5-rN-Benzyl-N-f4-fbutoxybenzvπaminocarbonvnbenzene-1.2.4-tr icarboxylic ar

The corresponding amine was prepared from 4-butoxybenzaldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 0.93 (t, J=8 Hz, 3H), 1.3-1.5 (m, 2 H), 1.6-1.8 (m, 2 H), 3.1-3.7 (m, 2 H), 3.8-4.0 (m, 2H), 4.0-4.2 (m, 2H), 6.7-7.7 (m, 11 H). MS (FAB)- m/e 504 (M-H)".

Example 193 5-rN-Benzyl-N-f4-f2-methoxyphenoxy benzvπaminocarbonyl1benzene-1.2.4- tricarboxylic acid The corresponding aldehyde was made from 2-methoxyphenol and 4- fluorobenzaldehyde. The amine was prepared from this aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 3.73 and 3.75 (2s, total = 3H) 4.0-4.6 (m, 4H), 6.7-6.8 (m, 2H), 7.0-7.4 (m, 12H), 7.6-7.7 (m, 1H). MS (FAB)" m/e 554 (M-H)".

Example 194 5-[N-Benzyl-N-(4-(4-benzyloxy)benzyl)aminocarbonyl]benzene-1 .2.4- tricarboxylic acid The corresponding amine was prepared from 4-benzyloxybenzaldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-de) δ 4.1-4.2 (m, 2H), 4.2-5.0 (m, 2H), 5.0-5.1 (m, 2H), 6.9-7.5 (m,15H). MS (FAB)" m/e 538 (M-H)".

Example 195

5-[N-Benzyl-N-(4-(2-methylphenoxy)benzyπaminocarbonyl]be nzene-1.2 _r 4- tricarboxylic acid The corresponding aldehyde was made from 2-methylphenol and 4- fluorobenzaldehyde. The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d _β ) δ 2.13

96/34851

-194-

and 2.17 (2s, total = 3H) 4.1-4.2 (m, 2H), 4.2-5.8 (m, 2H), 6.7-7.4 (m, 15H). MS (FAB)" m/e 538 (M-H)".

Example 196 5-fN-Benzyl-N-f3-methoxy-4-benzyloxybenzyπaminocarbonyl]ben zene-1.2.4- tricarboxylic acid The corresponding amine was prepared from 4-benzyloxy-3- methoxybenzaldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-dβ) δ 3.70 and 3.78 (2s, total = 3H), 4.1-4.2 (m, 2H), 4.2-4.9 (m, 2H), 5.00 and 5.05 (2s, totaϊ= 2H), 6.6-7.4 (m,15H). MS (FAB)- m/e 568 (M-H)".

Example 197 5-rN-Benzyl-N-f3-f2-benzylphenoxy)benzyπaminocarbonyl]benze ne-1.2.4- tricarboxylic acid

The corresponding aldehyde was prepared from 2- hydroxydiphenylmethane and 3-bromobenzaldehyde according to the procedure of Kodarna, et. al. (Kodama, M.; Shiobara, Y.; Sumitomo, H.; Matsumura, K.; Tsakamoto, M.; Harada, C. J. Org. Chem., 53, 72-77 (1988). The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 3.8-3.9 (m, 2H), 4.1-4.2 (m, 2H), 4.2-4.9 (m, 2H), 6.7-7.2 (m, 20H). MS (FAB)" m/e 614 (M-H) ^" .

Example 198

5-[N-Benzyl-N- 4-(3.4-dimethylphenoxy)benzyl)aminocarbonvnbenzene-1.2.4- tricarboxylic acid The corresponding aldehyde was prepared from3,4-dimethylphenol and 4-fluorobenzaldehyde. The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 2.20 (bs, 6H) 4.1-4.2 (m, 2H), 4.4 (d, J=7Hz, 2H), 7.0-7.4 (m,14H). MS (FAB)" m/e 552 (M-H)".

Example 199 5-rN-Benzyl-N-(4-(3.4-dimethylDhenoxy^benzvflaminocarbonvπb enzene-1.2.4- tricarboxylic acid The corresponding aldehyde was prepared from 2,4.6-trimethylphenol and 4-fluorobenzaIdehyde. The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-dβ) δ 1.9-2.0 (m, 9H) 4.0-4.2 (m, 2H), 4.0-4.8 (m, 2H), 6.5-6.7 (m, 2H), 6.9-7.4 (m,11H). MS (FAB)" m/e 566 (M-H)".

Example 200 5-rN-Benzyl-N-f4-f3.4-dimethylphenoxy^benzvflaminocarbonyl1b enzene-1.2.4- tricarboxylic acid The corresponding aldehyde was prepared from 4-methyIphenol and 4- fluorobenzaldehyde. The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-dβ) δ 2.29 (s, 3H) 4.1-4.2 (m, 2H), 4.2-4.9 (m, 2H), 6.8-7.4 (m,15H). MS (FAB)" m/e 538 (M- H) ^" .

Example 201 5-rN-Benzyl-N-f4-f3-methylphenoxy benzvhaminocarbonyllbenzene-1.2.4- tricarboxylic acid The corresponding aldehyde was prepared from 3-methylphenol and 4- fluorobenzaldehyde. The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 2.49 (s, 3H) 4.1-4.2 (m, 2H), 4.2-5.0 (m, 2H), 6.6-7.4 (m,15H). MS (FAB)- m/e 538 (M- H)-.

Example 202 5-fN-f2-Ethoxybenzyπ-N-f4-(4-benzylphenoxy)benzvπaminocarb onvnbenzene-

1.2.4-tricarboxylic acid The corresponding aldehyde was prepared from 4- hydroxydiphenylmethane and 4-fluorobenzaldehyde. The corresponding amine was prepared from the above aldehyde and benzylamine. The title compound was prepared by the procedures described in Example 188 ¹ H NMR (300 MHz, DMSO-d ₆ ) δ 1.1-1.2 (m, 3H), 3.8-5.0 (m, 8H), 6.8-7.4 (m,19H). MS (FAB)" m/e 658 (M-H)'.

Example 203 4-fN-Benzyl-N-( ^, 4-phenoxybenzyπaminocarbonylthioalkoxy benzene-1.2- dicarboxylic acid

Example 203A

Dimethyl 4-{fN.N-dimethylaminothiocarbonvπoxy)phthalate Dimethyl 4-hydroxyphthalate was converted to the title compound using the method described in J. Org. Chem., 31: 3980 (1966), and purified by chromatography using 98:2 CHCI ₃ -EtOAc. ¹ H NMR (CDCI ₃ ) δ 7.78 (d, 1H), 7.42 (d, 1 H), 7.25 (dd, 1 H), 3.91 , 3.90 (both s, total 6H), 3.45 (s, 3H), 3.35 (s, 3H). MS (DCI/NH3) m/e 298 (M+H) ⁺ , 315 (M+H+NH ₃ ) ⁺ .

Example 203B Dimethyl 4-mercaptophthalate The compound resulting from Example 203A was heated to 230 °C under N2 for 30 minutes to give dimethyl 4-{[N,N-dimethylaminocarbonyl]thio}- phthalate: ^" >H NMR (CDCI3) δ 7.86 (d, 1H), 7.71 (d, 1H), 7.67 (dd, 1H), 3.92, 3.90 (both s, total 6H), 3.07 (v. br. s, 6H). MS (DCI/NH3) m/e 298 (M+H) ⁺ , 315 (M+H+NH ₃ ) ⁺ . The above compound was saponified by the method described in Org.

Syn., Coll. Vol. VI, 825, then converted to the title compound by Fischer esterification. 1H NMR (CDCI3) δ 7.67 (d, 1H), 7.50 (d, 1H), 7.37 (dd, 1H), 3.92

(s,3H), 3.88 (s, 3H), 3.64 (s, 1H). MS (DCI/NH3) m/e 227 (M+H) ⁺ , 244 (M+H+NH ₃ ) ⁺ .

Example 203C Dimethyl 4-frN-benzyl-N-f4-phenoxybenzyπaminocarbonyl]thiolphthalate The compound resulting from Example 203B was converted to the title compound by the method of Example 38A. ¹ H NMR (CDCI3) δ 7.90 (m, 1H), 7.70 (m, 2H), 7.41-7.08 (envelope, 10H), 7.00 (br m, 4H), 4.57 (s, 2H), 4.51 (s, 2H), 3.92 (s, 6H). MS (DCI/NH3) m/e 559 (M+H+NH ₃ ) ⁺ .

Example 203D 4-{[N-Benzyl-N-(4-phenoxybenzvπaminocarbonvπthio)benzene-1 .2- dicarboxylic acid The title compound was prepared from the compound resulting from Example 203C by the method of Example 36C, except 1:1 hexane-EtOAc followed by 18:1 :1 EtOAc-H2θ-CH3Cθ2H was used for the chromatography. 1H NMR (DMSO-de) δ 7.80, 7.73, 7.38, 7.30, 7.15, 7.00 (all m, total 17H), 4.60 (v br s, 4H). MS (FAB-) m/e 512 (M-H)". Anal calcd for C29H23NO6S : C, 67.82; H, 4.51; N, 2.73. Found: C, 67.50; H, 4.51; N, 2.46.

Example 204 4-(2-fN-Benzyl-N-f4-phenoxybenzvπaminocarbonyπethyl)benzen e-1.2- dicarboxylic acid

Example 204A

Dimethyl 4-fdiazoacetvflmethylphthalate Dimethyl 4-carboxymethylphthalate (300 mg, 1.20 mmol) was dissolved in CH2CI2 (5 mL), then oxalyl chloride (167 mg, 115 μL, 1.32 mmol) was added, followed by a drop of DMF. After stirring at room temperature for 45 minutes, the reaction was concentrated, redissolved in CH3CN (2 mL), then added dropwise to a 0 °C mixture of CH3CN (2 mL) and trimethylsilyldiazomethane (1.3 mL of a 2 M in hexane solution, 2.60 mmol). The reaction was stirred at

0-5 °C for 3.5 hours, concentrated, and purified by chromatography eluting with 6:4 hexane-EtOAc to give 140 mg (42%) of the title compound as a dark brown, tacky solid. 1H NMR (CDCI ₃ ) δ 7.72 (d, 1H), 7.59 (d, 1H), 7.44 (dd, 1H), 5.18 (s, 1H), 3.92, 3.91 (both s, total 6H), 3.68 (s, 2H). MS (DCI/NH3) m/e 277 (M+H) ⁺ , 294 (M+H+NH ₃ ) ⁺ .

Example 204B Dimethyl 4-(2'-carbobenzoxy)ethylphthalate The resultant compound from Example 204A (135 mg, 0.49 mmol) in THF (2 mL) and benzyl alcohol (0.25 mL) was treated with a solution of silver benzoate in triethylamine (150 μL of 66 mg AgOBn in 1.3 mL triethylamine). After 1.5 hours, the reaction was concentrated and purified by chromatography eluting with 4:1 hexane-EtOAc to give 109 mg (62%) of the title compound as a clear and colorless oil. ^" >H NMR (CDCI3) δ 7.67 (d, 1H), 7.53 (d, 1H), 7.33 (m, 6H), 5.11 (s, 2H), 3.92, 3.91 (both s, total 6H), 3.04 (t, 2H), 2.70 (t , 2H). MS (DCI/NH3) m/e 357 (M+H) ⁺ , 374 (M+H+NH ₃ ) ⁺ .

Example 204C Dimethyl 4-(2'-carboxy)ethylphthalate The resultant compound from Example 204B (103 mg, 0.29 mmol) was dissolved in EtOAc (5 mL), then 10% Pd/C (30 mg) was added and the slurry stirred under H2 balloon for 2.5 hours. The reaction was filtered through celite and concentrated to give 75 mg (97%) of the title compound as a white crystalline solid. ^" "H NMR (CD3OD) δ 7.67 (d, 1H), 7.55 (d, 1H), 7.47 (dd, 1H), 3.86 (s, 3H), 3.84 (s, 3H), 2.98 (t, 2H), 2.63 (t , 2H). MS (DCI/NH3) m/e 267 (M+H) ⁺ , 284 (M+H+NH ₃ ) ⁺ .

Example 204D Dimethyl 4-(2'-[N-benzyl-N-f4-phenoxybenzvπaminocarbonvπethyl)phtha late The title compound was prepared from the compound resulting from

Example 204C by the method of Example 28C. ¹ H NMR (CDCI3) δ 7.65 (dd, 1 H), 7.50 (dd, 1 H), 7.33 (m, 6H), 7.20-6.92 (envelope, 9H), 4.59 (d, 2H), 4.40 (d,

2H), 3.90, 3.88 (both s, total 6H), 3.10 (m, 2H), 2.74 (m, 2H). MS (DCI/NH ₃ ) m/e 555 (M+H+NH ₃ ) ⁺ .

Example 204E 4-(2'-fN-Benzyl-N-(4-phenoxybenzvπaminocarbonyl1ethyl ⁾ benzene-1.2- dicarboxylic acid The title compound was prepared from the compound resulting from Example 204D by the method of Example 27D. ¹ H NMR (DMSO-d ₆ ) δ 7.59 (m, 1H), 7.50 (m, 1H), 7.45-7.10 (envelope, 11H), 6.97 (m, 4H), 4.50 (m, 4H), 2.97 (m, 2H), 2.78(m, 2H). MS (FAB+) m/e 510 (M+H)+ and (FAB-) m/e 508 (M-H)". Anal calcd for C31H27NO6 -0.25 H ₂ 0: C, 72.43; H, 5.39; N, 2.72. Found: C, 72.40; H, 5.18; N, 2.56.

Example 205 3-fN-Benzyl-N-(4-phenoxybenzvhaminocarbonyl1amino-6-carboxy- thioDhenol

Example 205A Methyl 4-([N-benzyl-N-f4-phenoxybenzyπaminocarbonyl1amino}-2- hydroxybenzoate Methyl 4-amino-2-hydroxybenzoate, prepared by treating 4- aminosalicylic acid with diazomethane, was converted to the title compound by the method of Example 34D, except no K2CO3 was added. ¹ H NMR (CDCI3) δ 10.78 (s, 1 H), 7.69 (d, 1 H), 7.42-7.27 (envelope, 9H), 7.13 (m, 1 H), 7.00 (m, 4H), 6.90 (dd, 1 H), 6.76 (d,1 H), 6.48 (s, 1 H), 4.60 (s, 4H), 3.90 (s, 3H). MS (DCI/NH3) m/e 483 (M+H) ⁺ .

Example 205B

Methyl 4-{fN-benzyl-N-f4-phenoxybenzyπaminocarbonvπaminoϊ-2-{fN. N- dimethylaminothiocarbonyl]oxy)benzoate The compound described in Example 205A was converted to the title compound by the method of Example 203A, except recrystallization from MeOH was used for the purification. ¹ H NMR (CDCI3) δ 7.90 (d, 1H), 7.42-7.22

(envelope, 11H), 7.13 (m, 1 H), 7.01 (m, 4H), 6.57 (s, 1H), 4.60 (d, 4H), 3.90 (s, 3H), 3.63 (s, 3H), 3.58 (s, 3H). MS (DCI/NH3) m/e 587 (M+H+NH ₃ ) ⁺ .

Example 205C Methyl 4-{[N-benzyl-N-f4-phenoxybenzvnaminocarbonvπaminol-2-{fN.N- dimethylaminocarbonvπthiolbenzoate The compound described in Example 205B was converted to the title compound by the method described in the first paragraph of Example 203B, except this compound was purified by chromatography using 1:1 hexane- EtOAc. 1H NMR (CDCI3) δ 7.86 (d, 1 H), 7.50 (dd, 1H), 7.42 (d,1 H), 7.40-7.22 (envelope, 9H), 7.13 (m, 1H), 7.01 (m, 4H), 6.61 (s, 1H), 4.58 (d, 4H), 3.10, 3.00 (both v br s, total 6H). MS (DCI/NH3) m/e 587 (M+H+NH ₃ ) ⁺ .

Example 205D 3-rN-Benzyl-N-(4-phenoxybenzvπaminocarbonvπamino-6-carboxy -thiophenol The compound described in Example 205C was converted to the title compound by the method described in Example 27D, except the reaction was heated under reflux for 2 hours instead of stirring at room temperature ovemight. Purification by preparative HPLC was required (Rainin Dynamax- 60A C18 column, using a gradient of 20-100% CH3CN vs 0.1% TFA in water), yielding both the title compound and the disulfide (Example 206). ¹ H NMR (DMSO-de) δ 8.88 (s,1H), 7.82 (d, 1H), 7.69 (d, 1H), 7.37, 7.25 (both m, total 10H), 7.13 (m, 1 H), 6.98 (m, 4H), 4.60 (s, 2H), 4.57 (s, 2H). MS (FAB+) m/e 485 (M+H)+. Anal calcd for C28H24N2O4S : C, 69.40; H, 4.99; N, 5.78. Found: C, 69.01 ; H, 4.79; N, 5.62.

Example 206 Bisf5-[N-Benzyl-N-(4-phenoxybenzyπaminocarbonvπamino-2-car boxyphenvπ disulfide The title compound was isolated as a biproduct in the preparation of the compound resulting from Example 205D. H NMR (DMSO-d ₆ ) δ 9.03 (s, 2H), 7.86 (m, 4H), 7.50 (dd, 2H), 7.38 (m, 4H), 7.30-7.10 (envelope, 16H), 6.96 (m, 8H), 4.50 (s, 4H), 4.44 (s, 4H). MS (FAB+) m/e 967 (M+H)+. Anal calcd for

C ₅ 6H46N4θ ₈ S2«0.50 H ₂ 0: C, 68.91 ; H, 4.85; N, 5.74. Found: C, 68.82; H, 4.67; N, 5.53.

Example 207 1.2-Difacetoxymethoxycarbonvh-4-{[N-benzyl-N-f4- phenoxybenzyl)aminocarbonyl1oxy)benzene The compound described in Example 38B (635 mg, 1.28 mmol) was dissolved in diglyme (10 mL), then triethylamine (657 mg, 0.90 mL, 6.50 mmol) was added. After 30 minutes, tetrabutylammonium iodide (200 mg, 0.54 mmol) and bromomethyl acetate (1.0 g, 6.5 mmol) were added. The reaction was stirred under N2 at 50-60 °C for 20 hours, then cooled and partitioned between water and EtOAc. The EtOAc layer was washed with brine, dried over Na2Sθ4, filtered and concentrated in vacuo. The crude material was purified by chromatography eluting with 7:3 hexane-EtOAc to afford 310 mg (38%) of the title compound as a tacky solid. ¹ H NMR (CDCI3) δ 7.83 (d, 1 H), 7.53, 7.37, 7.27, 7.13, 7.01 (all m, total 16H), 5.95 (s, 4H), 4.75 (m, 4H), 2.08, 2.07 (both s, total 6H). MS (FAB+) m/e 642 (M+H)+. Anal calcd for C35H31NO11: C, 65.52; H, 4.87; N, 2.18. Found: C, 65.22; H, 4.56; N, 1.92.

Example 208

5- ⁽ N- ⁽ 2-Ethoxybenzvπ-N-r3- ⁽ 3.4-dichlorophenoxy ⁾ benzyl]aminocarbonyl}- benzene-1.2.4-tricarboxylic acid N-2-Ethoxybenzyl-N-[3-(3,4-dichlorophenoxy)benzyl]amine was prepared using the same procedure as given for the preparation for N-benzyl- N-(4-phenoxybenzyl) amine except replacing the benzyl amine with 2- ethoxybenzyl amine and the 4-phenoxybenzaldehyde with 3-(3,4- dichlorophenoxy)benzaldehyde.

The title compound was prepared by the procedures described in Example 148 using N-(2-ethoxybenzyl)-N-[3-(3,4-dichlorophenoxy)benzyl]- amine in place of N-benzyl-N-(4-phenoxy)benzylamine. ¹ H NMR (DMSO-dε, 300 MHz), δ 1.20 (dt, 3H), 3.90 (dq, 2H), 4.25 (d, 2H), 4.65 (s,2H), 6.80 - 7.65 (m, 11 H), 7.70 (m, 1 H), 8.40 (m, 1 H). MS (FAB)+ m/e 639 (M+H)+ and (FAB)" m/e 637 (M-H)~-

Example 209 5-fN-Benzyl-N-(3-chloro-4-phenoxy benzylaminocarbonyl}benzene-1.2.4- tricarboxylic acid N-Benzyl-N-(4-phenoxy-2-chlorobenzyl)amine was prepared using the same procedure as given for the preparation for N-benzyl-N-(4-phenoxybenzyl) amine except replacing the 4-phenoxybenzaldehyde with 4-phenoxy-2- chlorobenzaldehyde.

The title compound was prepared by the procedures described in Example 148 using N-benzyl-N-(4-phenoxy-3-chlorobenzyl)amine in place of N-2-ethoxybenzyl-N-[3-(4-methylphenoxy)benzyl] amine . ¹ H NMR (DMSO-dβ, 300 MHz), δ 4.30 (d, 2H), 4.70 (b,2H), 6.80 - 7.45 (m, 13H), 7.80 (s, 1H), 8.35 (d, 1H). MS (FAB)+ m/e 560 (M+H)+ and (FAB)- m/e 558 (M-H)-.

Example 210

2-Amino-5-{N-benzyl-N-C4-phenoxy)benzylaminocarbonylmethy l)benzene-1.4- dicarboxylic acid

Example 210A Dimethyl 2-amino-5-allylterephthalate

The method described in Example 180B was used to convert allyltributyltin (9.93 g, 30 mmol) and the bromide resulting from Example 186A (8.07 g, 28.0 mmol) to the title compound (6.35 g, 91 %). ¹ H NMR (300 MHz, CDCI ₃ ) δ 7.73 (s, 1H), 7.14 (s, 1H), 5.97 (m, 1H), 5.64 ( br s, 2H), 4.98 (m, 2H), 3.89 (s, 3H), 3.87 (s, 3H), 3.58 (dt, 2H).

Example 21 OB 2-Allyl-5-benzyloxycarbonylamino-4-benzyloxycarbonylbenzoic acid methyl ester A solution of the compound resulting from Example 210A (4.72 g, 18.9 mmol), benzyl chloroformate (8.10 mL, 56.7 mmol) and

N.N-diisopropylethylamine (7.34 g, 56.7 mmol) in dioxane (70 mL) was refluxed for 15 hours. After cooling to room temperature, the reaction mixture was diluted with ether and hexane (25 mL each), filtered through silica gel (100 g), and rinsed with dichloromethane and ether (1:1 mixture). The filtrate was concentrated in vacuo, and the residue was purified by column chromatography eluting with 15% ethyl acetate in hexane to give the title compound (8.11 g, 81%) mixed with an inseparable impurity. H NMR (300 MHz, CDCI ₃ ) δ 10.40 (br s, 1 H), 8.93 (s, 1H), 7.93 (s, 1 H), 7.40 (m, 10H), 5.96 (m, 1 H), 5.36 (s, 2H), 5.22 (s, 2H), 5.01 (m, 2H), , 1H), 3.89 (s, 3H), 3.66 (dt, 2H).

Example 21 PC 2-{N-benzyl-N-f4-phenoxy)benzylaminocarbonylmethyl}-5- benzyloxycarbonylamino-4-benzyloxycarbonylbenzoic acid methyl ester A mixture of the compound resulting from Example 210B (5.51 g, 12.0 mmol), ruthenium dioxide (113 mg, 0.85 mmol) and sodium periodate (921.8 g, 102 mmol) in acetonitrile (20 mL), carbon tetrachloride (20 mL) and water (30 mL) was heated at 55 °C for 3 hours. The mixture was then allowed to cool to room temperature, filtered through Celite, and rinsed with water (30 mL) and ethyl acetate (150 mL). The organic phase of the filtrate was separated, washed with 10% aqueous sodium bisulfite (40 mL), water (40 mL) and brine (30 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo.

To the residue thus obtained and dissolved in dichloromethane (100 mL) was added N-benzyl-N-(4-phenoxy)benzylamine (4.95 g, 17.1 mmol), 4- dimethylaminopyridine (100 mg), and 1-(3-dimethylaminopropyl)-3- ethylcarbodiimide (3.26 g, 17.1 mmol). After 15 hours at room temperature; the reaction mixture was diluted with ether (200 mL), washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and concentrated in vacuo.

The residue was then purified by column chromatography eluting with 20% followed by 30% ethyl acetate in hexane to give the title compound (2.73 g, 3.65 mmol, 30%). ¹ H NMR (300 MHz, CDCI ₃ ) δ 10.45 (br s, 1H), 9.08, 9.07 (2 s's, 1H), 7.89,7.86 (2s's, 1H), 7.44-6.90 (m, 24H), 5.34,5.33 (2 s's, 2H), 5.24 (s, 2H), 4.61 , 4.60, 4.58, 4.56 (4 s's, 4H), 4.12, 4.09 (2 s's, 2H), 3.86, 3.85 (2 s's, 3H).

Example 210D 2-Amino-5-{N-benzyl-N-(4-phenoxy)benzylaminocarbonylmethyl}- 4- methoxycarbonylbenzoic acid

A mixture of the amide resulting from Example 210C (2.47 g, 3.30 mmol) and 10% palladium on carbon (1 g) in methanol (30 mL) was stirred under a hydrogen balloon for 4 hours. The mixture was then filtered through Celite, rinsed with methanol, and concentrated in vacuo to give the title compound (1.59 g, 92%). 1H NMR (300 MHz, DMSO-de) δ 7.58, 7.55 (2 s, 1 H), 7.43-7.20 (m, 10H), 7.14 (m, 1H), 6.98 (m, 4H), 4.62, 4.58 (2 s, 2H), 4.47, 4.45 (2 s, 2H), 4.01 , 3.97 (2 s, 2H), 3.71 ,3.70 (2 s, 3H). MS (FAB+) m/e 525 (M+H)+.

Example 210E 2-Amino-5-(N-benzyl-N-(4-phenoxy)benzylaminocarbonylmethyl)b enzene-1.4- dicarboxylic acid The method described in Example 173B was used to convert the compound resulting from Example 210D (221 mg, 0.42 mmol) to the title compound (212 mg, 98%). 1H NMR (300 MHz, DMSO-d ₆ ) δ 7.63, 7.60 (2 s, 1 H), 7.50-7.17 (m, 11H), 7.13 (m, 4H), 4.65, 4.62 (2 s, 2H), 4.53, 4.51 (2 s, 2H), 4.08, 4.04 (2 s, 2H). MS (FAB +) m/e 511 (M+H)+.

Examples 211-345 Using the procedures described in the schemes and preceeding examples, the following compounds can be prepared:

Using the procedures described in the schemes and preceeding examples, the following compounds can be prepared:

wherein

Rl is selected from the group consisting of nitro, hydroxy, ethyl, amino, methylamino, carboxy, methoxycarbonyl, ethoxycarbonyl, pentafluoroethoxy, methoxy, ethoxy, n-hexyloxy, cyano, carboxamido, fluoro, bromo, chloro, and iodo; and

R2 is selected from the group consisting of 4-methyl, 4-iodo, 4-chloro, 4-fluoro,

4-ethyl, 4-trifluoromethyl, 3,4-dichloro, 3,5-dichloro, 4-isopropyl, 3,4-diiodo, 3,5- diiodo, 3,4-difluoro, 3,5-difluoro, and 3,4,5-trifluoro.

Using the procedures described in the schemes and preceeding examples, the following compounds can be prepared:

wherein

Rl is selected from the group consisting of nitro, hydroxy, ethyl, amino, methylamino, carboxy, methoxycarbonyl, ethoxycarbonyl, pentafluoroethoxy, methoxy, ethoxy, n-hexyloxy, cyano, carboxamido, fluoro, bromo, chloro, and iodo;

R2 is selected from the group consisting of 4-methyl, 4-iodo, 4-chloro, 4-fluoro,

4-ethyl, 4-trifluoromethyl, 3,4-dichloro, 3,5-dichloro, 4-isopropyl, 3,4-diiodo, 3,5- diiodo, 3,4-difluoro, 3,5-difluoro, and 3,4,5-trifluoro; and

R3 and R4 are each independently selected from the group consisting of hydrogen, chloro, bromo, iodo, fluoro, methyl, methoxy, dichloro, difluoro, diiodo, and dimethyl.

Inhibition of Protein Famesyltransferase in vitro inhibition of protein famesyltransferase can be measured by the following procedure. (Procedures for determination of the inhibition of famesylation of the oncogene protein Ras are described by Goldstein, et al., J. Biol. Chem., 266:15575-15578 (1991) and by Singh in United States patent No. 5245061.)

Rat brain protein famesyltransferase activity was measured using an Amersham Life Science commercial scintillation proximity assay kit and substituting a biotin-K Ras B fragment (biotin-Lys-Lys-Ser-Lys-Thr-Lys-Cys-Val- He-Met-Cθ2H), 0.1 mM final concentration, for the biotin-lamin substrate provided by Amersham. The enzyme is purified according to Reiss, Y., et al., Cell, 62: 81-88 (1990), utilizing steps one through three. The specific activity of the enzyme used is approximately 10 nmol substrate famesylated/mg enzyme/hour. The percent inhibition of the famesylation caused by the compounds of the invention (at 1 x 10' ⁵ M) compared to an uninhibited control sample was evaluated in the same Amersham test system. The results for the compounds of the invention are shown in Table 1. The data show that the compounds of the invention are inhibitors of protein famesyltransferase.

Table 1 In vitro Inhibition of Protein Fa

The compounds of the present invention can be used in the form of salts derived from inorganic or organic acids. These salts include but are not limited to the following: acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, cyclopentanepropionate, dodecylsulfate, ethanesulfonate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxy- ethanesulfonate, lactate, maleate, methanesulfonate, nicotinate, 2- naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3- phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, p-toluenesulfonate and undecanoate. Also, the basic nitrogen-containing groups can be quatemized with such agents as loweralkyl halides (such as methyl, ethyl, propyl, and butyl chloride, bromides, and iodides), dialkyi sulfates like dimethyl, diethyl, dibutyl, and diamyl sulfates, long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides, aralkyl halides like benzyl and phenethyl bromides, and others. Water or oil-soluble or dispersible products are thereby obtained. Examples of acids which may be employed to form pharmaceutically acceptable acid addition salts include such inorganic acids as hydrochloric acid, sulphuric acid and phosphoric acid and such organic acids as oxalic acid, maleic acid, succinic acid and citric acid. Basic addition salts can be prepared in situ during the final isolation and purification of the compounds of formula (I), or separately by reacting the carboxylic acid function with a suitable base such as the hydroxide, carbonate or bicarbonate of a pharmaceutically acceptable metal cation or with ammonia, or an organic primary, secondary or tertiary amine. Pharmaceutically acceptable salts include, but are not limited to, cations based on the alkali and alkaline earth metals, such as sodium, lithium, potassium, calcium, magnesium, aluminum salts and the like, as well as nontoxic ammonium, quaternary ammonium, and amine cations, including, but not limited to ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, ethylamine, and the

like. Other representative organic amines useful for the formation of base addition salts include diethylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine and the like.

The compounds of the invention are useful (in humans and other mammals) for inhibiting protein famesyltransferase and the famesylation of Ras. These inhibitors of protein famesyltransferase are also useful for inhibiting or treating cancer in humans and other mammals. Examples of the kinds of cancers which may be treated with the compounds of the invention include, but are not limited to, carcinomas, such as lung, colorectal, exocrine pancreatic, cervical, esophageal, stomach, and small intestinal; sarcomas, such as oesteroma, osteosarcoma, lepoma, liposarcoma, hemanioma, and hemangiosarcoma; melanomas, such as amelanotic and melanotic; mixed types of cancers such as carcinosarcoma, lymphoid tissue type, follicular reticulum, cell sarcoma andHodgkins disease; and leukemias, such as myeloid, acute lymphoblastic, chronic lymphocytic, acute myloblastic and chronic mylocytic.

The ability of the compounds of the invention to inhibit or treat carcinoma can be demonstrated according to the methods of Mazerska Z., Woynarowska B., Stefanska B., Borowski S., Drugs Exptl. Clin. Res. 13(6), 345-351 (1987); Bissery, MC, Guenard F, Guerritte-Voegelein F, Lavelle F., Cancer Res. 51 , 4845-4852 (1991); and Rygaard J, and Povlsen C, Acta Pathol. Microbiol. Scand. 77, 758 (1969).

These inhibitors of protein famesyltransferase are also useful for treating or preventing restenosis in humans and other mammals. The ability of the compounds of the invention to prevent restenosis can be demonstrated according to the methods described by Kranzhofer, R. et al. Circ. Res. 73: 264- 268 (1993), Mitsuka, M. et al. Circ. Res. 23: 269-275 (1993) and Santoian, E.C. et al. Circulation 88: 11-14 (1993).

For use as a chemotherapeutic agent, the total daily dose administered to a host in single or divided doses may be in amounts, for example, from 0.01 to 500 mg/kg body weight daily, preferably in amounts from 0.1 to 20 mg/kg body weight daily and more preferably in amounts from 0.5 to 10 mg/kg body weight daily. Dosage unit compositions may contain such amounts of submultiples thereof to make up the daily dose.

For treatment or prevention of restenosis, the total daily dose administered to a host in single or divided doses may be in amounts, for example, from 0.001 to 1000 mg/kg body weight daily and more preferred from 1.0 to 50 mg/kg body weight daily. Dosage unit compositions may contain such amounts of submultiples thereof to make up the daily dose.

The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.

It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination, and the severity of the particular disease undergoing therapy. The compounds of the present invention may be administered orally, parenterally, sublingually, by inhalation spray, rectally, or topically in dosage unit formulations containing conventional nontoxic pharmaceutically acceptable carriers, adjuvants, and vehicles as desired. Topical administration may also involve the use of transdermal administration such as transdermal patches or iontophoresis devices. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrastemal injection, or infusion techniques.

Injectable preparations, for example, sterile injectable aqueous or oleagenous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1 ,3-propanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

Suppositoπes for rectal administration of the drug can be prepared by mixing the dru ; a suitable nonirritating excipient such as cocoa butter and polyethylene glycols which are solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum and release the drug. Solid dosage forms for oral administration may include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound may be admixed with at least one inert diluent such as sucrose, lactose, or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., lubricating agents such as magnesium stearate. In the case of capsules, tablets, and pills, the dosage forms may also comprise buffering agents. Tablets and pills can additionally be prepared with enteric coatings.

Liquid dosage forms for oral administration may include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs containing inert diluents commonly used in the art, such as water. Such compositions may also comprise adjuvants, such as wetting agents, emulsifying and suspending agents, and sweetening, flavoring, and perfuming agents.

The compounds of the present invention can also be administered in the form of liposomes. As is known in the art, liposomes are generally derived from phospholipids or other lipid substances. Liposomes are formed by mono- or multi-lamellar hydrated liquid crystals that are dispersed in an aqueous medium. Any non-toxic, physiologically aceptable and metabolizable lipid capable of forming liposomes can be used. The present compositions in liposome form can contain, in addition to a compound of the present invention, stabilizers, preservatives, excipients, and the like. The preferred lipids are the phospholipids and phosphatidyl cholines (lecithins), both natural and synthetic.

Methods to form liposomes are known in the art. See, for example, Prescott, Ed., Methods in Cell Biology. Volume XIV, Academic Press, New York, N.Y. (1976), p. 33 et seq. While the compounds of the invention can be administered as the sole active pharmaceutical agent for the treatment of cancer, they can also be used in combination with one or more other chemotherapeutic agents.

Representative examples of chemotherapeutic agents are described inHolleb, et al., Clinical Oncology. American Cancer Society, United States (1991) p 56 et seq. These agents include alkylating agents such as the nitrogen mustards (mechloethamine, melphalan, chlorambucil, cyclophosphamide and ifosfamide), nitrosoureas (carmustine, lomustine, semustine, streptozocin), alkyl sulfonates (busulfan), triazines (dacarbazine) and ethyenimines (thiotepa, hexamethylmelamine); folic acid analogues (methotrexate); pyrimidine analogues (5-fluorouracil, cytosine arabinoside); purine analogues (6- mercaptopurine, 6-thioguanine); antitumor antibiotics (actinomycin D, the anthracyclines (doxorubicin), bleomycin, mitomycin C, methramycin); plant alkaloids such as vinca alkaloids (vincristine, vinblastine) and etoposide (VP- 16); hormones and hormone antagonists (tamoxifen and corticosteroids); and miscellaneous agents (cisplatin, taxol, brequinar).

The above compounds to be employed in combination with the famesyl protein transferase inhibitor of the invention will be used in therapeutic amounts as indicated in the Physicians' Desk Reference (PDR) 47th Edition (1993), which is incoφorated herein by reference, or such therapeutically useful amounts as would be known to one of ordinary skill in the art.

The compounds of the invention and the other chemotherapeutic agent can be administered at the recommended maximum clinical dosage or at lower doses. Dosage levels of the active compounds in the compositions of the invention may be varied so as to obtain a desired therapeutic response depending on the route of administration, severity of the disease and the response of the patient. When administered as a combination, the therapeutic agents can be formulated as separate compositions which are given at the same time or different times, or the therapeutic agents can be given as a single composition. The foregoing is merely illustrative of the invention and is not intended to limit the invention to the disclosed compounds. Variations and changes which are obvious to one skilled in the art are intended to be within the scope and nature of the invention which are defined in the appended claims.