Title:
BIOLOGICAL BITS AS COMPUTABLE ELEMENTS WITHIN LIVING SYSTEMS
Document Type and Number:
WIPO Patent Application WO/2020/061257
Kind Code:
A3
Abstract:
Disclosed herein are protease-based biological circuits for use in the diagnosis and treatment of disease and disorders characterized by aberrant protease signaling. An exemplary method of treating disease in a subject includes administering to the subject a plurality of therapeutic agent loaded-liposomes, wherein each of the liposomes comprises a different density of peptides surrounding the core and a different dose of the therapeutic agent, wherein the peptides comprise a cleavage site for a protease of interest, and wherein cleavage of the peptides open the liposome and release the therapeutic agent from within the liposome.
Inventors:
HOLT BRANDON (US)
KWONG GABRIEL (US)
KWONG GABRIEL (US)
Application Number:
PCT/US2019/051833
Publication Date:
March 25, 2021
Filing Date:
September 19, 2019
Export Citation:
Assignee:
GEORGIA TECH RES INST (US)
International Classes:
A61K47/65; C12Q1/37; G01N33/68; G16B5/20
Foreign References:
| US7344856B1 | 2008-03-18 |
Other References:
BASEL MATTHEW T. ET AL.: "Protease-Sensitive, Polymer-Caged Liposomes: A Method for Making Highly Targeted Liposomes Using Triggered Release", ACS NANO, vol. 5, no. 3, 22 March 2011 (2011-03-22), pages 2162 - 2175, XP055807444, DOI: 10.1021/nnl03362n
SARKAR NIHAR ET AL.: "Matrix Metalloproteinase-Assisted Triggered Release of Liposomal Contents", BIOCONJUGATE CHEMISTRY, vol. 19, no. 1, 2008, pages 57 - 64, XP055225328, DOI: 10.1021/bc070081p
HOLT BRANDON ALEXANDER ET AL.: "Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics", JOVE, vol. 137, 2018, pages 1 - 6, XP055693648, DOI: 10.3791/57937
PITA MARCOS ET AL.: "Enzyme logic gates for assessing physiological conditions during an injury: Towards digital sensors and actuators", SENSORS AND ACTUATORS B: CHEMICAL, vol. 139, no. 2, 2009, pages 631 - 636, XP026138624, DOI: 10.1016/j.snb. 2009.03.00 1
EDGINGTON LAURA E. ET AL.: "Functional imaging of proteases: recent advances in the design and application of substrate-based and activity-based probes", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 15, no. 6, 2011, pages 798 - 805, XP028338677, DOI: 10.1016/j.cbpa. 2011.10.01 2
SARKAR NIHAR ET AL.: "Matrix Metalloproteinase-Assisted Triggered Release of Liposomal Contents", BIOCONJUGATE CHEMISTRY, vol. 19, no. 1, 2008, pages 57 - 64, XP055225328, DOI: 10.1021/bc070081p
HOLT BRANDON ALEXANDER ET AL.: "Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics", JOVE, vol. 137, 2018, pages 1 - 6, XP055693648, DOI: 10.3791/57937
PITA MARCOS ET AL.: "Enzyme logic gates for assessing physiological conditions during an injury: Towards digital sensors and actuators", SENSORS AND ACTUATORS B: CHEMICAL, vol. 139, no. 2, 2009, pages 631 - 636, XP026138624, DOI: 10.1016/j.snb. 2009.03.00 1
EDGINGTON LAURA E. ET AL.: "Functional imaging of proteases: recent advances in the design and application of substrate-based and activity-based probes", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 15, no. 6, 2011, pages 798 - 805, XP028338677, DOI: 10.1016/j.cbpa. 2011.10.01 2
Attorney, Agent or Firm:
VORNDRAN, Charles et al. (US)
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