Title:
BIOLOGICAL SEED COATING AGENT BASED ON TRICHODERMA-BACILLUS SEQUENTIAL INOCULATION SYMBIOTIC CULTURE FERMENTATION LIQUOR, AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/268093
Kind Code:
A1
Abstract:
Disclosed are a biological seed coating agent based on a trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor, and a preparation method therefor. According to the principle of synthetic biology, a symbiotic bacterium solution is prepared, by means of a staged inoculation co-fermentation technology, from trichoderma having characteristics such as inducing disease resistance and stress resistance, and bacillus having characteristics such as antagonizing pathogenic bacteria and promoting crop growth. The symbiotic bacterium solution is mixed with diatomite and brassinolide in proportion to prepare a powdered biological seed coating agent. The biological seed coating agent comprises high-content spores, antagonistic substances, growth-promoting substances and other substances.
Inventors:
CHEN JIE (CN)
LIU PENG (CN)
WANG XINHHUA (CN)
LIU PENG (CN)
WANG XINHHUA (CN)
Application Number:
PCT/CN2022/100273
Publication Date:
December 29, 2022
Filing Date:
June 22, 2022
Export Citation:
Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
C12N1/14; A01N63/22; A01N63/38; A01P3/00; C12N1/20; C12N3/00; C12N11/14; C12P17/10; C12P21/02; C12R1/07
Domestic Patent References:
| WO2019155252A1 | 2019-08-15 |
Foreign References:
| CN113444649A | 2021-09-28 | |||
| CN110938562A | 2020-03-31 | |||
| CN109536411A | 2019-03-29 | |||
| US20160278384A1 | 2016-09-29 | |||
| CN101358176A | 2009-02-04 |
Other References:
KARUPPIAH VALLIAPPAN, VALLIKKANNU MURUGAPPAN, LI TINGTING, CHEN JIE: "Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity", MICROBIAL CELL FACTORIES, vol. 18, no. 1, 1 January 2019 (2019-01-01), pages 1 - 16, XP093016420, DOI: 10.1186/s12934-019-1233-7
WU QIONG, NI MI, DOU KAI, TANG JUN, REN JIANHONG, YU CHUANJIN, CHEN JIE: "Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield", MICROBIAL CELL FACTORIES, vol. 17, no. 1, 1 January 2018 (2018-01-01), pages 1 - 12, XP093016423, DOI: 10.1186/s12934-018-1004-x
KARUPPIAH VALLIAPPAN, SUN JIANAN, LI TINGTING, VALLIKKANNU MURUGAPPAN, CHEN JIE: "Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity", FRONTIERS IN MICROBIOLOGY, vol. 10, 1 January 2019 (2019-01-01), pages 1 - 16, XP093016425, DOI: 10.3389/fmicb.2019.01068
CHEN ZE-NAN;ZOU TIAN;WANG ZHI-WEI: "Research Rrogress on Preventing Plant Diseases and Insect Pests of Seed Coating Formulation", JOURNAL OF ANHUI AGRICULTURAL SCIENCES, vol. 46, no. 32, 27 November 2018 (2018-11-27), pages 10 - 13, XP093016433, ISSN: 0517-6611, DOI: 10.13989/j.cnki.0517-6611.2018.32.004
WU QIONG, NI MI, DOU KAI, TANG JUN, REN JIANHONG, YU CHUANJIN, CHEN JIE: "Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield", MICROBIAL CELL FACTORIES, vol. 17, no. 1, 1 January 2018 (2018-01-01), pages 1 - 12, XP093016423, DOI: 10.1186/s12934-018-1004-x
KARUPPIAH VALLIAPPAN, SUN JIANAN, LI TINGTING, VALLIKKANNU MURUGAPPAN, CHEN JIE: "Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity", FRONTIERS IN MICROBIOLOGY, vol. 10, 1 January 2019 (2019-01-01), pages 1 - 16, XP093016425, DOI: 10.3389/fmicb.2019.01068
CHEN ZE-NAN;ZOU TIAN;WANG ZHI-WEI: "Research Rrogress on Preventing Plant Diseases and Insect Pests of Seed Coating Formulation", JOURNAL OF ANHUI AGRICULTURAL SCIENCES, vol. 46, no. 32, 27 November 2018 (2018-11-27), pages 10 - 13, XP093016433, ISSN: 0517-6611, DOI: 10.13989/j.cnki.0517-6611.2018.32.004
Attorney, Agent or Firm:
DUAN&DUAN LAW FIRM (CN)
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