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Title:
BIORREMEDIATE PROCESS WITH BACTERIAL, FUNGIC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL
Document Type and Number:
WIPO Patent Application WO/2018/170561
Kind Code:
A1
Abstract:
The present invention relates to a product developed from the use of bacteria, fungi and enzymes for biopolymerization of a specific medium constituted of celluiosic material. The action of bacteria, fungi and enzymes leads to structural modifications of the celluiosic material, resulting in a material with high fioatability degree and ability to adsorb contaminants. Consequently, this material can be used for the bioremediation of carbonaceous molecules in liquid substances and in soils, where the polluting agent is transformed into an composite totally non¬ toxic to the environment.

Inventors:
WALVAG KOLFINN ROGER (BR)
PEREIRA LUIZ ANTONIO ALVES (BR)
Application Number:
PCT/BR2017/000023
Publication Date:
September 27, 2018
Filing Date:
March 22, 2017
Export Citation:
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Assignee:
WALVAG KOLFINN ROGER (BR)
PEREIRA LUIZ ANTONIO ALVES (BR)
International Classes:
B09C1/10; C12P19/04; C02F3/34; C12R1/01; C12R1/645
Domestic Patent References:
WO2012008841A12012-01-19
WO1995026944A11995-10-12
Foreign References:
EP0594125A21994-04-27
US5738703A1998-04-14
US5821113A1998-10-13
BRPI0703623A22009-05-19
US5656490A1997-08-12
Other References:
KUMAR, A. K. ET AL.: "Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review", BIORESOURCES AND BIOPROCESSING, vol. 4, no. 1, January 2017 (2017-01-01), pages 1 - 19, XP055550640
DZIONEK, A. ET AL.: "Natural carriers in bioremediation: A review", ELECTRONIC JOURNAL OF BIOTECHNOLOGY, vol. 23, 2016, pages 28 - 36, XP055550642, Retrieved from the Internet
BAYAT, Z. ET AL.: "Immobilization of Microbes for Bioremediation of Crude Oil Polluted Environments: A Mini Review", OPEN MICROBIOL J., vol. 9, 2015, pages 48 - 54, XP055550644
SHAHSAVAN, E. ET AL.: "Plant residues - A low cost, effective bioremediation treatment for petrogenic hydrocarbon-contaminated soil", SCIENCE OF THE TOTAL ENVIRONMENT, vol. 443, 2013, pages 766 - 774, XP055550646, Retrieved from the Internet
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Claims:
CLAIM

"B!ORREMEDIATE PROCESS WITH BACTERIAL, FUNGiC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL".

the present Application for Patent Privilege of Invention refers to an innovative product developed from the use of bacteria, fungi and enzymes for bio- polymerization of a specific midst with adsorbent/biodegradation characteristics for the adsorption/bioremediation using as substrate a midst constituted of materials composed of cellulose where there is a reaction in synthesis creating conditions of expressiveness for the bioremediation of carboniferous molecules in liquid substances and in soils transforming the polluting agent into an organ composite totally non-toxic to the environment.

Patent claim 1: Constitute a process of total drying (75%) of the substrate. When it is made from the oven drying of materials based on cellulose (light woods derived from pruning residues and peat);

Patent claim 2: Constitute a process of grinding (fragmentation) of the material. When grinding the cellulose based material in granularity between 1.0 / 3.0 mm;

Patent claim 3: Constitute the addition of pre-defined proportions of bacteria, fungi and enzymes natural polymer based on specific doses;

Patent claim 4: Constitute the final processing of structural physical modification of the product result of enzyme addition to the polymeric substrate

Description:
"BIORREMEDIATE PROCESS WITH BACTERIAL, FUNGIC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL".

DESCRIPTION

The present application for patent refers to an innovative product developed from the use of bacteria, fuirigi and enzymes for the bio-polymerization of a specific midst with adsorbent/biodegradation characteristics using as substrate a means constituted of materials composed of cellulose where there is a reaction in synthesis creating conditions of expressiveness for the bioremediation of carboniferous moiecuies.

The necessity of this development becomes evident, when we face a reality of permanent risk to the environment owing to pollution by liquid substances containing carboniferous moiecuies caused by human failures or by breakdown of systems and equipment.

The products currently used for the absorption of liquid carbonic substances from the environment when accidents happen, are the use of materials with absorption power.

However many of these materials have the drawback of substantially increasing the volume of waste function of its non-biodegradation or by a slow process. Sometimes, causing greater damage to the environment causing in some cases (as in water) the sinking of the oil owing to increase their densities and/or affinity for water environment.

Also, occurring in certain cases, the leaching process (liberation of the initially absorbed oils), causing the return of pollutants to the environment and when in the soil prevents the mechanical action of the oils in the processes of gas exchange between the free 0 2 and ground substrate. The use of naturally polymeric bacteria, fungi arid enzymes using as substrate a midst constituted of materials composed of cellulose demonstrate significant advantages over the above listed products, because when it is of interest of the recovery of the oily substances it has the property of transforming them in a very short time, a totally non-toxic compound info the means by the action of said enzymes in this specific substrate (by its characteristics promotes an action of absorbency in the carboniferous molecules realizing its addition to its environment and eliminating immediately the aggressive characteristics to the ecosystem), of lower density than water (Even when associated with oils) And for being chemically non-polar by its vegetal characteristic (Suffering total rejection by the water) ensure an good buoyancy, preventing by these factors the reintroduction of the polluting element to the water environment. When in the soil it prevents the mechanical action of the oils in the processes of gas exchange and when in the soil it prevents the mechanical action of the oils in the processes of gas exchange between the free O* and the terrestrial environment when the pollutant is still in the contaminating state and transforming it into an organ composite totally non-toxic to the environment.

The production technology of the present invention has four distinct phases, namely:

1) Process drying/lyophilization substrate - This phase is preceded by drying materials based on cellulose (derived from softwoods and pruning residues of peat);

2) Grinding (fragmentation) of the material - This phase is preceded grinding the cellulose-based material in granularity between 3.0 / 8.0mm; 3) A process of adding bacteria and fungi biodecompositores - Alter grinding of the components adding suitable percentage (2% to 5%) of Tioxidans and oxidizing bacteria of the sort. Thiobaciilus Ferroxidans, Thiobacii!us Thiooxidans, SC27;

4) Addition in pre-defined proportions of natural base polymer enzymes -

Specific doses of: Oxirredutases, Hydrolases, Ligases and Isomerases on culture basis;

5) Final processing - Structural physical modification of the product result of enzyme addition to the polymeric substrate.

The object of the present Application for Patent Privilege of Invention, entitled BIORREMEDIATE PROCESS WITH BACTERIAL, FUNGIC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL obviousness an innovative product developed from the use of bacteria, fungi and enzymes for bio-polymerization of a specific midst with adsorbent/biodegradation characteristics for the adsorption/bioremediation of carboniferous liquid substances of the environment in water, soil, with superior efficiency technologies and existing products. Being its substrate conceived in granular form promoting the addition to its means of oily liquid contaminants with a reason of the weight in the range among 100% to 200%. That by the characteristics of its basic substances does not present any degree of toxicity, High degree of buoyancy, and transforming the pollutant into a completely nontoxic organ compound. In the present application we come up with the technological innovation of manipulation of the bacterial, fungal and enzymatic elements, using as substratum a midst constituted by materials composed of cellulose using them of form and specific purpose.