Title:
BIOSENSOR COMPRISING A MODIFIED BETA-LACTAMASE AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/012487
Kind Code:
A1
Abstract:
Provided is a protein including a polypeptide having at least 95%sequence homology with an amino acid sequence selected from a group consisting of SEQ ID NOs: 1 to 5, where the amino acid at position 70 of SEQ ID NOs: 1-5 must be cysteine. Also provided are a biosensor including the protein and methods of using the biosensor for identifying a beta-lactam and a beta-lactamase inhibitor.
Inventors:
LEUNG YUN-CHUNG (CN)
WONG KWOK-YIN (CN)
CHUNG SAI-FUNG (CN)
WONG KWOK-YIN (CN)
CHUNG SAI-FUNG (CN)
Application Number:
PCT/CN2023/106980
Publication Date:
January 18, 2024
Filing Date:
July 12, 2023
Export Citation:
Assignee:
UNIV HONG KONG POLYTECHNIC (CN)
International Classes:
C07K1/13; C07K14/00; C12N15/55; C12N15/65; G01N33/68
Domestic Patent References:
| WO2012105596A1 | 2012-08-09 |
Foreign References:
| US20050261270A1 | 2005-11-24 | |||
| CN107530406A | 2018-01-02 | |||
| KR20160039706A | 2016-04-12 | |||
| US20180023072A1 | 2018-01-25 | |||
| CN109337889A | 2019-02-15 |
Other References:
HU, J.P.; ZUO, KE; WAN, HUA: "Advance Research on NDM-l: Structure, Catalytic Mechanism and Inhibitors", JOURNAL OF CHENGDU UNIVERSITY (NATURAL SCIENCE EDITION), CHINA, vol. 36, no. 2, 30 June 2017 (2017-06-30), China, pages 115 - 124, XP009552617, ISSN: 1004-5422
DATABASE Protein 13 March 2016 (2016-03-13), KUMAR, M.: "beta-lactamase NDM-3 [Acinetobacter baumannii]", XP093128503, retrieved from NCBI Database accession no. AMO51457.1
TASSONI RAFFAELLA, BLOK ANNELOES, PANNU NAVRAJ S., UBBINK MARCELLUS: "New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis", BIOCHEMISTRY, vol. 58, no. 7, 19 February 2019 (2019-02-19), pages 997 - 1009, XP093128504, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b01085
DATABASE Protein 13 March 2016 (2016-03-13), KUMAR, M.: "beta-lactamase NDM-3 [Acinetobacter baumannii]", XP093128503, retrieved from NCBI Database accession no. AMO51457.1
TASSONI RAFFAELLA, BLOK ANNELOES, PANNU NAVRAJ S., UBBINK MARCELLUS: "New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis", BIOCHEMISTRY, vol. 58, no. 7, 19 February 2019 (2019-02-19), pages 997 - 1009, XP093128504, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b01085
Attorney, Agent or Firm:
BEIJING PAT SF INTELLECTUAL PROPERTY AGENCY CO. LTD (CN)
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