Title:
BIOSYNTHETIC PRODUCTION OF 2-FUCOSYLLACTOSE
Document Type and Number:
WIPO Patent Application WO/2022/040411
Kind Code:
A3
Abstract:
The present invention provides a novel biosynthetic production process which converts L-galactose into 2'-fucosyllactose via four enzymatically catalyzed reaction steps. The present process is designed such that co-factors required by the process are regenerated within the four reaction steps, hence making the process cost-effective and efficient. The process can be performed in vitro in a cell-free system. The present invention also provides mutant enzymes that can be used to increase production levels of 2'-fucosyllactose, whether using the novel pathway described herein or the mannose-dependent pathway known in the art.
Inventors:
MAO GUOHONG (US)
VALLIERE MEAGHAN (US)
WU JOHNSON (US)
JOHNSON SEAN (US)
YU OLIVER (US)
VALLIERE MEAGHAN (US)
WU JOHNSON (US)
JOHNSON SEAN (US)
YU OLIVER (US)
Application Number:
PCT/US2021/046659
Publication Date:
April 21, 2022
Filing Date:
August 19, 2021
Export Citation:
Assignee:
CONAGEN INC (US)
MAO GUOHONG (US)
VALLIERE MEAGHAN (US)
WU JOHNSON (US)
JOHNSON SEAN ROBERT (US)
YU OLIVER (US)
MAO GUOHONG (US)
VALLIERE MEAGHAN (US)
WU JOHNSON (US)
JOHNSON SEAN ROBERT (US)
YU OLIVER (US)
International Classes:
C07K14/34; C12N15/52; C12N15/77
Domestic Patent References:
| WO2020048927A1 | 2020-03-12 | |||
| WO2015175801A1 | 2015-11-19 |
Foreign References:
| US20170081353A1 | 2017-03-23 |
Other References:
DATABASE UniProtKB UniProt; 22 November 2017 (2017-11-22), ANONYMOUS : "SubName: Full=Glycosyl transferase family 11 {ECO:0000313|EMBL:SFH87266.1};", XP055934589, Database accession no. A0A1I3DKP6
OHYAMA C,ET AL: "Molecular cloning and expression of GDP-D-mannose-4,6-dehydratase, a key enzyme for fucose metabolism defective in Lec13 cells.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 273, no. 23, 5 June 1998 (1998-06-05), US , pages 14582 - 14587, XP002328593, ISSN: 0021-9258, DOI: 10.1074/jbc.273.23.14582
OHYAMA C,ET AL: "Molecular cloning and expression of GDP-D-mannose-4,6-dehydratase, a key enzyme for fucose metabolism defective in Lec13 cells.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 273, no. 23, 5 June 1998 (1998-06-05), US , pages 14582 - 14587, XP002328593, ISSN: 0021-9258, DOI: 10.1074/jbc.273.23.14582
Attorney, Agent or Firm:
GE, Zhiyun et al. (US)
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