Title:
BIOTHIOL DETECTING COMPOSITION COMPRISING REDOX REGULATION PROTEIN
Document Type and Number:
WIPO Patent Application WO/2018/084607
Kind Code:
A1
Abstract:
The present invention relates to a biothiol detecting composition comprising a redox regulation protein, a method for detecting biothiols by using the same, and a biosensor/kit for detecting biothiols. The present invention provides the effect of rapidly measuring free biothiols in body fluids. In addition, relative content ratios and changes of total to free biothiols in body fluids can be detected in real time, which allows biothiols to be available as main indices of diseases through which prediction and warning can be made against various diseases. Further, because various redox stress changes associated with main diseases can be accounted for by variations of biothiols, the present invention can provide important technical, economical, and social values for the investigation of pathogenesis mechanisms and the diagnosis of diseases in the future.
Inventors:
KIM YOUNG-PIL (KR)
LEE JIN OH (KR)
LEE JIN-WON (KR)
YANG YOON MO (KR)
KIM TAE-WUK (KR)
LEE JIN OH (KR)
LEE JIN-WON (KR)
YANG YOON MO (KR)
KIM TAE-WUK (KR)
Application Number:
PCT/KR2017/012344
Publication Date:
May 11, 2018
Filing Date:
November 02, 2017
Export Citation:
Assignee:
UNIV HANYANG IND UNIV COOP FOUND (KR)
International Classes:
G01N33/536; G01N33/533; G01N33/68
Foreign References:
| KR101535750B1 | 2015-07-09 | |||
| JP4288334B2 | 2009-07-01 |
Other References:
SUZUKI, Y. J. ET AL.: "18]Redox Regulation of DNA-Protein interactions by Biothiois", METHODS IN ENZYMOLOGY, vol. 252, 1995, pages 175 - 180
POOLE, L. B.: "The Basics of Thiols and Cysteines in Redox Biology and Chemistry", FREE RADICAL BIOLOGY AND MEDICINE, (ELECTRONIC PUBLISHING, vol. 80, 27 November 2014 (2014-11-27), pages 148 - 157, XP055482538
GROITL, B. ET AL.: "Thiol-based Redox Switches", BIOCHIMICA ET BIOPHYSICA ACTA , (ELECTRONIC PUBLISHING, vol. 1844, no. 8, 19 March 2014 (2014-03-19), pages 1335 - 1343, XP028849468
PARK, K. S. ET AL.: "A Label-free Method for Detecting Biological Thiols Based on Blocking of Hg2+-Quenching of Fluorescent Gold Nanoclusters", BIOSENSORS AND BIOELECTRONICS, (ELECTRONIC PUBLISHING, vol. 45, 4 February 2013 (2013-02-04), pages 65 - 69, XP055482543
POOLE, L. B.: "The Basics of Thiols and Cysteines in Redox Biology and Chemistry", FREE RADICAL BIOLOGY AND MEDICINE, (ELECTRONIC PUBLISHING, vol. 80, 27 November 2014 (2014-11-27), pages 148 - 157, XP055482538
GROITL, B. ET AL.: "Thiol-based Redox Switches", BIOCHIMICA ET BIOPHYSICA ACTA , (ELECTRONIC PUBLISHING, vol. 1844, no. 8, 19 March 2014 (2014-03-19), pages 1335 - 1343, XP028849468
PARK, K. S. ET AL.: "A Label-free Method for Detecting Biological Thiols Based on Blocking of Hg2+-Quenching of Fluorescent Gold Nanoclusters", BIOSENSORS AND BIOELECTRONICS, (ELECTRONIC PUBLISHING, vol. 45, 4 February 2013 (2013-02-04), pages 65 - 69, XP055482543
Attorney, Agent or Firm:
DANA PATENT LAW FIRM (KR)
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