| CLAIMS: 1. A seed of Brassica carinata cultivar designated AG 044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015. 2. A plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of claim 1. 3. A Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of the plant of claim 2 when grown in the same location under the same environmental conditions. 4. A tissue culture of protoplasts or regenerable cells of the plant, or part thereof, of claim 2. 5. A method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is the plant of claim 2. 6. A method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing the plant of claim 2 with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of claim 2 is either a female parent or a male parent. 7. A method of producing a Brassica carinata variety produced from the plant of claim 2, wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (e) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (f) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (g) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (h) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D. 8. The method of claim 7, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions. 9. The method of claim 7 or 8, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism. 10. A plant, or part thereof, or seed thereof, produced by the method of any one of claims 5-9. 11. A seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014. 12. A plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of claim 11. 13. A Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of the plant of claim 12 when grown in the same location under the same environmental conditions. 14. A tissue culture of protoplasts or regenerable cells of the plant, or part thereof, of claim 12. 15. A method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is the plant of claim 12. 16. A method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing the plant of claim 12 with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of claim 12 is either a female parent or a male parent. 17. A method of producing a Brassica carinata variety produced from the plant of claim 12, wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (e) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (f) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (g) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (h) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22. 18. The method of claim 17, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions. 19. The method of claim 17 or 18, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism. 20. A plant, or part thereof, or seed thereof, produced by the method of any one of claims 15-19. |
[0001] 1. FIELD
[0002] The invention is in the field of Brassica carinata breeding (i.e. Ethiopian mustard breeding), specifically relating to the inbred Brassica carinata cultivar designated Brassica carinata AG 044-312D and Brassica carinata AGR044-3A22.
[0003] 2. BACKGROUND
[0004] Brassica carinata is a member of the Brassicaceae (formerly Cruciferae) family, commonly known as the mustard family. In Canada, Brassica carinata is commonly known as carinata, Ethiopian mustard or Abyssinian mustard. It is also referred to as gomenzer (Getinet 1996).
[0005] The genus Brassica is a member of the tribe Brassiceae in the mustard family (Brassicaceae; Warwick et al. 2009). In addition to B. carinata, the Brassica genus includes several economically important oilseed crop species: B. juncea (L). Czern. (brown mustard), B. napus L. (rape, Argentine canola), B. nigra (L.) W.D.J. Koch (black mustard), and B. rapa L. (field mustard, Polish canola). The genus Brassica also includes B. oleracea L. food crops, including cabbage, broccoli, cauliflower, Brussels sprouts, kohlrabi and kale.
[0006] The six Brassica species are closely related genetically, as described in the Triangle of U
(U 1935). Brassica carinata is an amphidiploid (BBCC, 2n=34) thought to be derived from interspecific hybridization of the diploid species B. nigra L. (BB, 2n=16) and B. oleracea L. (CC, 2n=18; Prakash et al. 2012). The native range of Brassica carinata comprises the central highland region of Ethiopia. All of the naturally occurring carinata in these regions is cultivated; there do not appear to be wild populations
[0007] Brassica carinata is an herbaceous annual with a determinate growth habit (Zanetti et al. 2013). Carinata plants have an erect, upright bearing, averaging 1.4 m in height. Plants are highly branching, with well-developed and aggressive tap root systems (Barro and Martin 1999).
[0008] Seeds are globose, 1-1.5 mm in diameter and finely reticulated (Mnzava and Schippers 2004). They vary from yellow to yellow-brown to brown in colour (Getinet 1987; Rahman and Tahir 2010). The seeds are rich in oil, containing 37-44% depending on the cultivar and growing conditions, and the seed protein content is high, at 25-30% epressed as seed dry weight (Pan et al, 2012)
[0009] In Spain and Italy, seed oil is used for biofuel (Bouaid et al. 2005; Cardone et al. 2002, 2003; Gasol et al. 2007, 2009) and as a bio-industrial feedstock with many uses (i.e., in lubricants, paints, cosmetics, plastics). In North America, carinata has been assessed as a biofuel feedstock (Blackshaw et al. 2011), and crude oil produced from B. carinata seed has been used for the production of green bio- diesel and bio-jet fuel. In October 2012, experimental aviation flights by the National Research Council of Canada using the world's first 100% bio-jet fuel were successful ("ReadiJet 100% biofuels flight - one of 2012's 25 most important scientific events", Popular Science Magazine, 2012(12).
[0010] Carinata is grown as a cover crop to reduce soil erosion and herbicide use, and promote water conservation in orchards (Alcantara et al. 2011); it can be plowed into the soil for use as a green manure soil amendment, or as a bio-fumigant (Lazzarini et al; Pane et al. 2013). Carinata plants also have utility in heavy metal phytoremediation (Mourato et al 2015). Also, carinata seed meal can be used as a high protein animal feed when mixed with other protein sources fed to beef cattle (Schulmeister et al, 2015) [0011] (A) Brassica carinata Breeding
[0012] Carinata breeding has focused on the improvement of seed quality traits, agronomic performance (e.g., early maturity, higher yield) and disease resistance. Carinata varieties optimized for production of feedstock for biofuels (i.e. biodiesel, bio-jet fuel), bio-industrial uses such as for manufacturing of bio-plastics (Newson et al, 2014, Impallomeni et al. 2011), lubricants (Zannetti et al; 2006) and specialty fatty acids such as 5, 13-docosadienoic acid, 5-eicosenoic acid (Jadhav et al. 2005), eicosapentaenoic acid (Cheng et al. 2009) and nervonic acid (Taylor et al 2010) have been developed. In some cases, modification of the seed oil profile has involved the use of transgenic technologies to introduce specific genes encoding enzymes of the fatty acid biosynthesis pathways (Taylor 2010).
[0013] Breeding to improve carinata seed quality has led to development of lines with lower meal glucosinolate content with potential for use as a protein rich additive for additive in animal diets (Xin and Yu 2014; Getinet et al. 1997; Marquez-Lema et al. 2006, 2008). Carinata bred for very high levels of glucosinolates in seed meal is of use in biofumigation (Marquez-Lema et al. 2009).
[0014] Critical to the development and breeding of any crop is the ability to make use of genetic and phenotypic diversity. For a newly developing crop such as carinata, it is necessary to be able to obtain a sufficient pool of genetic material to identify genetic backgrounds more adapted to target geographies (i.e. a better starting point), as well as variation for traits of interest. This allows for crossing or other modifications to be done, to identify genetic combinations superior to the types already tested. Thus, an important initial objective is the collection and characterization of as large of a collection of genetic backgrounds as possible, for each target geography.
[0015] Characterization of accessions or breeding lines of carinata generally takes place in the field in the geography of interest, where phenotypic data collected is generally more reflective of actual performance that would be realized by a seed producer. Traits collected focus on those that would be of agronomic or economic benefit in the crop. Examples of traits characterized in a carinata breeding program are early plant vigor, plant height, branching habit, days to flower, silique density, flower petal color, pod size, reaction to heat and water stress, disease susceptibility, shatter tolerance, etc.
[0016] Breeding nurseries are often the first cycle of evaluation of new material, whether from sources external to the breeding program, or new genetic combinations generated within the breeding program. Generally, nurseries utilize single or paired rows with frequent checks (i.e. the best available commercial germplasm for a specified geography), to evaluate overall agronomic potential as well as specific traits of the experimental material. For material that is somewhat heterogeneous but displays traits of interest, often multiple single plants will be self-pollinated, and carried forward to the next breeding cycle. In this way, multiple streams of inbred parents are in simultaneous development each year. These inbred lines can be yield tested to become an open-pollinated (OP) variety if sufficiently improving on the checks; however, the more usual scenario is they are used as parents in crossing or as parents in hybrid testing. When inbred lines are developed in this way as a result of a planned cross, this is referred to as pedigree breeding. In the case that they are developed from a collection or accession, it is usually referred to as inbred line development. Self-pollination is also done in completely inbred lines to preserve a pure seed supply of those lines that may warrant further testing. [0017] Crossing of inbred lines, by sexual hybridization, is typically done manually in controlled conditions. The selection of parents of these crosses is critical to the effectiveness of a breeding program. Parental lines are selected based on breeding priorities and the unique combination of traits available in potential crossing parents. Often, two or three rounds of crossing are needed to accumulate beneficial alleles into a single genetic background. This includes evaluating offspring of a cross, selecting the most desirable inbred lines as future parents, and making the next round of parental selection based on priority targets.
[0018] Doubled Haploid (DH) Technology: DH technology allows for the generation of completely homozygous lines, which are a combination of genes of the parental lines, in a single generation. This accelerates the process of inbred line development dramatically; as the process from seeding of parental lines to obtaining seed for a resulting inbred population from those parents will generally take about 18 months. To achieve a highly homozygous line using traditional self-pollination generally will take five or six growth cycles; which in the case of carinata would represent three years or six growth cycles, with two cycles completed per year. In this technique, using appropriate in vitro conditions, haploid microspores from an Fl plant can be induced to differentiate into diploid embryos and subsequently plantlets. This technique typically relies on a percentage of regenerated plants to undergo spontaneous doubling (usually in the range of 20 to 60% of plants, depending on a number of factors), whereas the remaining plants will remain haploid and sterile. To increase the efficiency of space used for seed increase, such as in the greenhouse or field, a flow cytometer is used to distinguish at an early stage the chromosome content "n" or "2n" of each plant. Thus, the sterile plants can be discarded at an early stage.
[0019] Molecular Markers: A breeding program can make use of marker assisted breeding (MAB) to accelerate the successful outcome of a breeding project. This allows the identification of lines carrying a trait of interest in the laboratory, while other lines not containing a marker of interest can then be discarded at an early stage. This increases the efficiency of a program, as the lines being evaluated in the field have a greater probability of meeting seed quality or other criteria. MAB relies on the existence of a dense set of genetic markers for the species of interest. Genetic markers are the unique sequences that may be found in allelic forms of genes, distinguishing one allele from another. Like genes themselves they can be transmitted to progeny in a Mendelian fashion and can thus be used to follow the movement of specific alleles from parents to progeny. There are several types of genetic markers in common use, including:
i. Restriction fragment length polymorphisms (RFLP): where a mutation may create or eliminate a restriction site, creating restriction fragments of unique size. These are detected by electrophoresis and transfer onto membranes (Southern blotting) followed by hybridization with specific labeled probes; ii. Random Amplified Polymorphic DNA (RAPD): where a single primer is used to PC amplify random segments of genomic DNA, sequence polymorphisms which diminish the primers ability to hybridize at a locus will cause the disappearance of specific bands, polymorphisms which result in improved hybridization can result in new amplified bands;
iii. Amplified Fragment Length Polymorphism (AFLP): where genomic DNA is digested with a pair of restriction enzymes, one rare cutting and the other frequent cutting. Fragments are then annealed to synthetic adaptors and then PCR carried out using primers complementary to the adaptor sequence and adjacent restriction sites. The PC products can be separated by gel or capillary electrophoresis and the pattern of bands produced constitutes a unique fingerprint for a particular genotype;
iv. Simple Sequence Repeats (SSRs): repeat sequences called microsatellites (from 2 nt to 6 nt in length) are found randomly distributed in plant genomes. The copy numbers of these repeats at their sites of occurrence can vary greatly and constitutes a source of considerable allelic polymorphism. Primers homologous with regions that flank known microsatellite-containing regions are used to amplify the genomic DNA and the variation in size of the polymorphic products can be used to demonstrate the local allelic variations;
v. Single Nucleotide Polymorphism (SNP): are single base changes, deletions or insertions that can occur in coding noncoding or intergenic DNA. These can be detected in a number of ways, for example by NGS sequencing, differential hybridization etc.
[0020] The most useful markers are those that are co-dominant - they allow a researcher to distinguish whether the plant is homozygous, heterozygous or null for a particular marker. The utility of a marker is also increased if it can be detected using very little DNA and if the methodology of detection is relatively simple, sensitive and reproducible. Allelic markers are also more valuable if their presence is linked to the expression of a particular phenotypic trait and molecular markers that co-segregated with alleles that are linked to quantitative trait loci (QTL) afford the opportunity to use these markers in marker assisted breeding.
[0021] Wide Crossing (interspecific Crossing): In some instances, a trait may not reside within the species of interest. In that case, it may be possible to transfer the trait via interspecific or wide crossing. The methodology for performing the cross is similar to that described for within-species crosses described above. However, unlike intraspecific crosses, the likelihood of the cross producing viable seed is very low and thus represents a formidable challenge to the success of this technique. In order to overcome this potential block, embryo rescue techniques are often employed to recover viable offspring from the cross. Essentially this relies on the progeny surviving until the embryogenic stage at which point it can be dissected from the silique and placed into artificial growth medium. Under appropriate conditions the cultured embryo can survive and be induced to differentiate into a plantlet, which can be grown into a mature plant. Successive rounds of embryo rescue may be needed until inbred progeny, or backcross-derived progeny, are stable and can produce fertile offspring without intervention. Often molecular markers, where available, are used to trace a specific allele from a related species into an adapted background in the target species, using repeated cycles of backcrossing.
[0022] Hybrid Cultivar Testing: Brassica species have been shown to display significant levels of heterosis, or hybrid vigor. Most of the B. napus varieties available today are hybrid varieties. It is very probable that similar levels of heterosis for seed yield and vigorous plant development can be exploited in B. carinata. This breeding technique may allow the crop to achieve yield potentials, which would allow the resulting biofuels to be more price competitive with traditional petroleum. However, not much research has been done to date on testing hybrid combinations of carinata. Thus, there may be a large forum for exploiting heterosis in this crop and increasing productivity on farm, as well as profitability to producers. [0023] In order to produce and test hybrid combinations, some form of pollination control system must be employed. The most widely used pollination control system to achieve this in Brassica species is the method of Ogura (1968) based on male cytoplasmic sterility. In cytoplasmic male sterile (cms) plants, a mitochondrial gene mutation interferes with the flowers ability to produce viable pollen but does not affect the functionality of the flower's female components. Because the mutation is within the mitochondrial genome, it is transmitted maternally through the cytoplasm. The Ogura cms trait is derived from a Japanese radish variety and has been successfully transferred to Brassica juncea and B. napus through interspecific crossing. While the mitochondrial gene mutation eliminates the ability of the cms variety to self-pollinate, it does not interfere with its ability to outcross via pollination from non- cms varieties and thus produce F 1 seed. However, the resulting hybrid F 1 produced in such a cross would be male sterile, as this trait is maternally transmitted. The cms phenotype in the F 1 can be overcome by the expression of a nuclear genome encoded restorer ( f) gene in the male variety used in the cross to produce the Fi. The nuclear Rf gene of radish has also been successfully introgressed in Brassica napus (ref) and Brassica juncea (ref) varieties. More recently, both the CMS and Rf traits have been developed and are being validated in B. carinata. The development of a set of genetically diverse cms and Rf lines in carinata will allow the testing of a large number of combinations for the first time in this Brassica species.
[0024] In a hypothetical production scheme based on the Ogura CMS system the following steps are carried out:
i. Based on genetic diversity and groupings, key morphological traits, and agronomic potential, female (A-lines) carrying the cms trait, and male (R-lines) carrying the restorer gene, are developed; ii. Test hybrids are produced and evaluated typically in replicated small plot trials, for evaluation of yield potential and other characteristics;
iii. Patterns of heterosis between parental "pools" are identified, combinations using these pools are tested more extensively;
iv. Specific combinations are also selected for potential commercial release as a new variety;
v. Parental seed is increased for commercial production scheme of best hybrid variety. In order to do so, a maintainer (B-line) of the cms parent is also necessary for seed increase.
[0025] Mutagenesis: Another method of creating genetic variation, and capturing beneficial changes in a heritable fashion, is through mutagenesis breeding. This method is often carried out via chemical means or ionizing radiation, and is typically focused either on a microspore or on a whole seed level. In Brassica breeding, some common forms of mutagens used have been chemical agents such as Ethyl methanesulfonate (EMS) or N-ethyl-N-nitrosourea (ENU), high levels of ionizing (x- ray or gamma) irrradiation or exposure to UV light. EMS produces random point mutations via low frequency methylation of guanine residues in genomic DNA. This results in altered Watson-Crick base pairing such that the affected base pairing is converted from G-C to A-T. ENU is also an alkylating agent that preferentially modifies thymine residues converting A-T to G-C. Ionizing radiation may affect DNA in many ways but more often than not the mutations are double strand breaks leading to deletions and frameshift mutations that are frequently inactivating.
[0026] To carry out this technique, seedlings or microspores are exposed to the mutagenic agent and the surviving fraction are allowed to develop into mature plants. In some cases, the mutagenized plantlets or embryos (in the case of microspore mutagenesis) may be exposed to selection in order to enrich for a particular phenotype. For example, mutagenesis has been used to develop plants that are resistant to the actions of specific herbicides; in this instance the developing plantlets or microspores can be grown in vitro in the presence of the herbicide(s) of interest in order to select for those plants with the appropriate mutations conferring resistance. The advantage of the microspore mutagenesis of the seed approach is that the resultant DH plants can be used to derive pure and homozygous plant lines, where all induced mutations, whether dominant or recessive, would be expressed. Mutagenesis has been used to develop brassica varieties with resistance to various herbicides, altered seed oil profiles and increased tolerance to disease and abiotic stress.
[0027] Genetic Transformation: In instances where unique and valuable traits are known to be available in distant plant or in non-plant species that cannot be transferred to Brassica carinata via classical breeding, and where the genes for those traits have been cloned, a breeding program may resort to genetic transformation techniques to stably transfer those genes into this species. Transfer of cloned genetic elements into B. carinata have been achieved via a number of means, including PEG mediated DNA uptake into protoplasts (Johnson, C M. et al. 1989), electroporation into protoplasts (Fromm, M et al 1985), ballistic infiltration using DNA coated microprojectiles (Finer, J. et al 1999), Agrobacteria-based vector infiltration (Babic et al, 1997), infection using plant virus based vectors (Gleba et al 2014). Aside from having the genes of interest in cloned form, the other requirements include having the genes cloned into a suitable vector to allow for their propagation in an appropriate bacterial system as well as their packaging, in appropriate viral and agrobacterial strains to allow for their infectious route of transfer. Once transferred they would also require appropriate plant based promoters, enhancers and terminators to allow for the correct temporal and tissue specific pattern of expression for the heterologous gene. Finally, in order to select those rare events where the heterologous gene expression unit has been successfully transferred into the plant genome, a selectable marker may be introduced, either physically linked to the heterologous gene of interest or co- transformed with the gene of interest at a suitable ratio so as to favor co-insertion.
[0028] The selectable marker may consist of a gene that can confer resistance to a particular herbicide or antibiotic that would otherwise kill the plant, or a gene that may confer a growth advantage, a gene that may alter a response to plant hormones, or may express a fluorescent protein that can allow transformed cells to be easily visualized. Examples of selectable markers based on conferring resistance to antibiotics, successfully used in brassica transformation are the NPTII gene (Bevan, 1984; Datla et al, 1992, encoding an enzyme conferring resistance to the antibiotic kanamycin and the HPT gene encoding an enzyme conferring resistance to the antibiotic Hygromycin ( othstein et al, 1987). Examples of selection markers based on conferring tolerance to herbicides and successfully used in brassica transformation are the BAR (Thompson et al, 1987)and PAT (Wohlleben et al, 1988) gene products which confer resistance to glufosinate (bialaphos) or L-PPT and the AHAS gene product conferring resistance to imidazolinones (Miki et al. 1990). Other plant selectable markers have been developed whose actions are not based on conferring resistance to toxic compounds per se but instead allow survival in the presence of nutrients not normally metabolized by the wildtype organism.
[0029] Transformation cannot only be used to introduce heterologous genes into the genome of carinata plants, it can also be used to introduce DNA constructs that are designed to modulate the expression of endogenous genes. Genes encoding antisense RNA or RNAI sequences (Tang and Galil, 2004) can be used to interfere or knock down the expression of endogenous genes to extremely low levels, simulating the effect of a null mutation at the endogenous locus. This of course relies on the continuous stable expression of the antisense or RNAi to be effective. In amphidiploid brassica species such as napus, juncea and carinata multiple copies of genes from the contributing ancestral species may create a high level of functional redundancy such that a single mutation in one of the homologues may not be sufficient to confer a phenotype. However, by using an RNAi or antisense approach, where the interfering RNA is derived from conserved sequences, one may conceivably be capable of targeting all of the expressed homologues and achieving a functional knockdown effect.
[0030] More recently several novel approaches have been developed which offer the ability to manipulate the plant genome in a targeted way. Collectively known as gene editing technology, (Petolino et al, 2010; Sauer et al 2014, Woo et al, 2015) the technologies share several important similarities:
i. They offer the ability to introduce small or large gene deletions in the plant genome by means of targeted double strand breaks at precise genomic locations
ii. They allow for the insertion of small inactivating mutations into specific genes of interest iii. They permit the replacement of an entire gene or gene segment by a modified counterpart iv. They allow for the insertion of a heterologous gene in a specific genomic location which may represent a preferred site for regulated gene expression, (i.e. downstream of a known endogenous promoter/enhancer)
[0031] As is evident by the examples cited and to those skilled in the art, there are numerous methods available to the carinata breeder to assemble a unique collection of traits into a singular cultivar. The skill lies in the ability of the breeder to select for the combination of traits through successive generations, and ultimately obtaining a unique, homogeneous and homozygous cultivar breeding true for the selected combination of traits, a necessary requirement for commercial crop production.
[0032] 3. SUMMARY
[0033] According to the present invention, there are provided novel Brassica carinata varieties designated Brassica carinata AGR044-312D and AGR044-3A22. This invention thus relates to the seeds of the AGR044-312D and AGR044-3A22 varieties, to plants of the AGR044-312D and AGR044-3A22 varieties, methods for producing a Brassica carinata plant produced by self- crossing of AGR044-312D or AGR044-3A22 or outcrossing said varieties with other Brassica carinata lines as well as producing DH varieties from Fl of said crosses, methods for producing a Brassica carinata plant by outcrossing the AGR044-312D and AGR044-3A22 with other brassica species, such as Brassica napus, Brassica juncea, Brassica oleracea, Brassica rapa, Brassica nigra, followed by backcrossing with AGR044-312D and AGR044-3A22 as well as producing DH varieties from said interspecific crosses. The invention also relates to the use of the AGR044-312D and AGR044-3A22 varieties as a background for chemical and/ or radiation induced mutagenesis or for targeted gene editing, for modulation of traits via RNA
interference or antisense RNA expression or for introduction of traits via genetic transformation. The invention may also encompass the use of the AGR044-312D and AGR044-3A22 line in a hybrid variety production scheme as described above. [0034] In the embodiments described below, "essentially all of the physiological and morphological characteristics" can mean, for example, the quantitative physiological and morphological characteristics described in Tables 1 and 3-7 for AGR044-3A22 or in Tables 1, 2, and 4-14 for AGR044-312D when grown in the same location under the same environmental conditions, as determined at the 5% significance level.
[0035] In the embodiments described below, "essentially all of the physiological and morphological characteristics" can also mean the quantitative physiological and morphological characteristics described in Tables 1, 3 and 5-7 for AGR044-3A22 or in Tables 1, 2, 5-10, and 12-14 for AGR044-312D when grown in the same location under the same environmental conditions, as determined at the 5% significance level.
[0036] In particular embodiments, there is provided:
1. A seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
2. A plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of embodiment 1.
3. The plant part of embodiment 2, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
4. The plant part of embodiment 3, wherein the plant part is pollen.
5. The plant part of embodiment 3, wherein the plant part is an ovule.
6. A Brassica carinata plant, or parts thereof,having essentially all of the physiological and morphological characteristics of the plant of embodiment 2 when grown in the same location under the same environmental conditions.
7. A tissue culture of protoplasts or regenerable cells of the plant, or part thereof, of embodiment 2.
8. The tissue culture according to embodiment 7, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks.
9. A Brassica carinata plant regenerated from the tissue culture of embodiment 7 or 8, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, when grown in the same location under the same environmental conditions.
10. A regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of the cultivar AGR044-312D when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from the plant or part thereof of embodiment 2.
11. A method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is the plant of embodiment 2.
12. A Brassica carinata seed produced by the method of embodiment 11.
13. A method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing the plant of embodiment 2 with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of embodiment 2 is either a female parent or a male parent.
14. The method of embodiment 13 wherein the plant of embodiment 2 is the female parent.
15. The method of embodiment 13 wherein the plant of embodiment 2 is the male parent.
16. An Fl hybrid seed produced by the method of any one of embodiments 13 to 15.
17. An Fl hybrid plant grown from the Fl hybrid seed produced by the method of any one of embodiments 13 to 15.
18. A method for producing a Doubled Haploidy variety comprising:
(a) isolating a flower bud of the Fl plant of embodiment 17;
(b) dissecting out a haploid microspore;
(c) placing the haploid microspore in culture;
(d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet;
(e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and
(f) continuing to grow the plantlet if it contains a diploid chromosome number.
19. The method of embodiment 18 further comprising inducing chromosome doubling by chemical or physical means.
20. A plant, or part thereof, or seed of a Doubled Haploidy variety produced by the method of embodiment 18 or 19.
21. A method of producing a Brassica carinata variety produced from the plant of embodiment 2, wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of:
(a) crossing a plant of cultivar AG 044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and
(d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
22. The method of embodiment 21, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
23. The method of embodiment 21 or 22, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
24. The method of embodiment 23, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
25. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of any one of embodiments 22 to 24.
26. A method of producing a Brassica carinata variety produced from the plant of embodiment 2, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D.
27. The method of embodiment 26, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
28. The method of embodiment 26 or 27, wherein the DNA construct comprises a transgene.
29. The method of embodiment 26 or 27, wherein the DNA construct comprises an RNAi construct.
30. The method of any one of embodiments 26 to 29, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D, when grown in the same location under the same environmental conditions. 31. The method of any one of embodiments 26 to 30, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
32. The method of embodiment 31, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
33. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of any one of embodiments 26 to 32.
34. A method of producing a Brassica carinata variety produced from the plant of embodiment 2, wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of:
(a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) selfing the progeny plants that have the desired trait to produce further progeny seed; and
(d) growing the further progeny seed and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
35. The method of embodiment 34, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
36. The method of embodiment 34 or 35, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
37. The method of embodiment 36, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
38. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of any one of embodiments 34 to 37.
39. A method of producing a Brassica carinata variety produced from the plant of embodiment 2, wherein the Brassica carinata variety comprises a new trait, the method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
40. The method of embodiment 39, wherein the mutagenic agent is ethyl
methanesulfonate, N-ethyl-N-nitrosourea, or x-ray, gamma or ultraviolet radiation.
41. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of embodiment 39 or 40.
42. A method of producing a carinata variety produced from the plant of embodiment 2, wherein the carinata variety comprises a desired trait, the method comprising:
(a) crossing a plant of cultivar AG 044-312D with a plant of another Brassicaceae species comprising the desired trait;
(b) using embryo rescue techniques to recover viable Fl plants from the cross or
growing Fl seeds to produce Fl plants;
(c) selfing the Fl plants that have the desired trait and carinata character;
(d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants;
(e) selfing the F2 plants that have the desired trait and carinata character;
(f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants;
(g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and
(h) selecting the progeny plants with the desired trait and carinata character to
produce the carinata variety produced from cultivar AGR044-312D.
43. The method of embodiment 42, wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
44. The method of embodiment 42 or 43, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
45. The method of embodiment 44, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
46. A plant, or part thereof, or seed of a progeny carinata variety produced by the method of any one of embodiments 42 to 45. 47. A method of producing a commercial plant product, the method comprising growing the plant of embodiment 2 to produce a commercial crop, and producing said commercial plant product from the commercial crop.
48. The method of embodiment 47, wherein the commercial plant product comprises oil, meal, or protein isolate.
49. A commercial plant product produced by the method of embodiment 47 or 48.
50. Oil, meal, or protein isolate produced by the method of embodiment 48.
51. Crushed, non-viable seed of Brassica carinata cultivar AG 044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123015.
52. A cell of a seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123015.
53. A cell of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed defined in embodiment 52.
54. A protoplast of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed defined in embodiment 52.
55. The cell of embodiment 53, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, or a stalk.
56. The cell of embodiment 55, wherein the plant part is pollen.
57. The cell of embodiment 55, wherein the plant part is an ovule.
58. A cell of a Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of the plant defined in embodiment 53 when grown in the same location under the same environmental conditions.
59. A tissue culture of protoplasts or regenerable cells of the plant, or part thereof, defined in embodiment 53.
60. The tissue culture according to embodiment 59, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks.
61. A cell of a Brassica carinata plant regenerated from the tissue culture of embodiment 59 or 60, wherein said plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
62. A cell of a regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from the plant or part thereof defined in embodiment 53.
63. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce seed, wherein the seed is produced by self-fertilization or cross-fertilization.
64. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015 to produce an Fl hybrid Brassica carinata seed, wherein the plant is either a female parent or a male parent in a cross-fertilization.
65. The use of embodiment 64 wherein the plant is the female parent.
66. The use of embodiment 64 wherein the plant is the male parent.
67. A cell of an Fl hybrid plant grown from the Fl hybrid seed produced by the use according to any one of embodiments 64 to 66.
68. A cell of an Fl hybrid plant grown from Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed.
69. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Doubled Haploidy variety.
70. Use of embodiment 69, wherein chromosome doubling is introduced by chemical or physical means.
71. A cell of a Doubled Haploidy variety produced from Brassica carinata cultivar AGR044- 312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
72. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, 73. The use of embodiment 72, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
74. The use of embodiment 73, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
75. A cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AG 044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of:
(a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and
(d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
76. The cell of embodiment 75, wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
77. The cell of embodiment 75 or 76, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
78. The cell of embodiment 77, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
79. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct.
80. The use of embodiment 79, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
81. The use of embodiment 79 or 80, wherein the DNA construct comprises a transgene.
82. The use of embodiment 79 or 80, wherein the DNA construct comprises an NAi construct.
83. The use of any one of embodiments 79 to 82, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
84. The use of any one of embodiments 79 to 83, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
85. The use of embodiment 84, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
86. A cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D.
87. The cell of embodiment 86, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
88. The cell of embodiment 86 or 87, wherein the DNA construct comprises a transgene.
89. The cell of embodiment 86 or 87, wherein the DNA construct comprises an RNAi construct.
90. The cell of any one of embodiments 86 to 89, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions. 91. The cell of any one of embodiments 86 to 90, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
92. The cell of embodiment 91, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
93. A cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of:
(a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) selfing the progeny plants that have the desired trait to produce further progeny plants; and
(d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
94. The cell of embodiment 93, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
95. The cell of embodiment 93 or 94, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
96. The cell of embodiment 95, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
97. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a new trait, wherein the new trait is introduced by exposing seedlings or microspores to a mutagenic agent.
98. The use of embodiment 97, wherein the mutagenic agent is ethyl methanesulfonate, N- ethyl-N-nitrosourea, or x-ray, gamma or ultraviolet radiation. 99. A cell of a plant of a Brassica carinata variety produced from cultivar AG 044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a new trait, and wherein the Brassica carinata variety is produced by a method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
100. The cell of embodiment 99, wherein the mutagenic agent is ethyl methanesulfonate, N- ethyl-N-nitrosourea, or x-ray, gamma or ultraviolet radiation.
101. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait.
102. The use of embodiment 101, wherein the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
103. The use of embodiment 101 or 102, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
104. The use of embodiment 103, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
105. A cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising:
(a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae
species comprising the desired trait;
(b) using embryo rescue techniques to recover viable Fl plants from the cross or
growing Fl seeds to produce Fl plants;
(c) selfing the Fl plants that have the desired trait and carinata character;
(d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants;
(e) selfing the F2 plants that have the desired trait and carinata character;
(f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants;
(g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and
(h) selecting the progeny plants with the desired trait and carinata character to
produce the carinata variety produced from cultivar AGR044-312D. 106. The cell of embodiment 105, wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AG 044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
107. The method of embodiment 105 or 106, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
108. The method of embodiment 107, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
109. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop.
110. Use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial plant product.
111. The use of embodiment 110, wherein the commercial plant product comprises oil, meal, or protein isolate.
112. A plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
113. The plant part of embodiment 112, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
114. A seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
115. A cell of a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
116. The cell of embodiment 115, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast. 117. A cell of a seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
118. A seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
119. A plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of embodiment 118.
120. The plant part of embodiment 119, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
121. The plant part of embodiment 120, wherein the plant part is pollen.
122. The plant part of embodiment 120, wherein the plant part is an ovule.
123. A Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of the plant of embodiment 119 when grown in the same location under the same environmental conditions.
124. A tissue culture of protoplasts or regenerable cells of the plant, or part thereof, of embodiment 119.
125. The tissue culture according to embodiment 124, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks.
126. A Brassica carinata plant regenerated from the tissue culture of embodiment 124 or 125, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, when grown in the same location under the same environmental conditions.
127. A regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of the cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from the plant or part thereof of embodiment 119.
128. A method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is the plant of embodiment 119.
129. A Brassica carinata seed produced by the method of embodiment 128. 130. A method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing the plant of embodiment 119 with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of embodiment 119 is either a female parent or a male parent.
131. The method of embodiment 130 wherein the plant of embodiment 119 is the female parent.
132. The method of embodiment 130 wherein the plant of embodiment 119 is the male parent.
133. An Fl hybrid seed produced by the method of any one of embodiments 130 to 132.
134. An Fl hybrid plant grown from the Fl hybrid seed produced by the method of any one of embodiments 130 to 132.
135. A method for producing a Doubled Haploidy variety comprising:
(a) isolating a flower bud of the Fl plant of embodiment 134;
(b) dissecting out a haploid microspore;
(c) placing the haploid microspore in culture;
(d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet;
(e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and
(f) continuing to grow the plantlet if it contains a diploid chromosome number.
136. The method of embodiment 135 further comprising inducing chromosome doubling by chemical or physical means.
137. A plant, or part thereof, or seed of a Doubled Haploidy variety produced by the method of embodiment 135 to 136.
138. A method of producing a Brassica carinata variety produced from the plant of embodiment 119, wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of:
(a) crossing a plant of cultivar AG 044-3A22 with another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and
(d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22. 139. The method of embodiment 138, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AG 044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
140. The method of embodiment 138 or 139, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
141. The method of embodiment 140, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
142. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of any one of embodiments 138 to 141.
143. A method of producing a Brassica carinata variety produced from the plant of embodiment 119, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22.
144. The method of embodiment 143, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
145. The method of embodiment 143 or 144, wherein the DNA construct comprises a transgene.
146. The method of embodiment 143 or 144, wherein the DNA construct comprises an RNAi construct.
147. The method of any one of embodiments 143 to 146, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22, when grown in the same location under the same environmental conditions.
148. The method of any one of embodiments 143 to 147, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
149. The method of embodiment 148, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba. 150. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of any one of embodiments 143 to 149.
151. A method of producing a Brassica carinata variety produced from the plant of embodiment 119, wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of:
(a) crossing a plant of cultivar AG 044-3A22 with another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) selfing the progeny plants that have the desired trait to produce further progeny seed; and
(d) growing the further progeny seed and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22.
152. The method of embodiment 151, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
153. The method of embodiment 151 or 152, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
154. The method of embodiment 153, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
155. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of any one of embodiments 151 to 154.
156. A method of producing a Brassica carinata variety produced from the plant of embodiment 119, wherein the Brassica carinata variety comprises a new trait, the method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
157. The method of embodiment 156, wherein the mutagenic agent is ethyl
methanesulfonate, N-ethyl-N-nitrosourea, or x-ray, gamma or ultraviolet radiation.
158. A plant, or part thereof, or seed of a Brassica carinata variety produced by the method of embodiment 156 or 157. 159. A method of producing a carinata variety produced from the plant of embodiment 119, wherein the carinata variety comprises a desired trait, the method comprising:
(a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait;
(b) using embryo rescue techniques to recover viable Fl plants from the cross or
growing Fl seeds to produce Fl plants;
(c) selfing the Fl plants that have the desired trait and carinata character;
(d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants;
(e) selfing the F2 plants that have the desired trait and carinata character;
(f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants;
(g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and
(h) selecting the progeny plants with the desired trait and carinata character to
produce the carinata variety produced from cultivar AGR044-3A22.
160. The method of embodiment 159, wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
161. The method of embodiment 159 or 160, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
162. The method of embodiment 161, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
163. A plant, or part thereof, or seed of a progeny carinata variety produced by the method of any one of embodiments 159 to 162.
164. A method of producing a commercial plant product, the method comprising growing the plant of embodiment 119 to produce a commercial crop, and producing said commercial plant product from the commercial crop.
165. The method of embodiment 164, wherein the commercial plant product comprises oil, meal, or protein isolate.
166. A commercial plant product produced by the method of embodiment 164 or 165.
167. Oil, meal, or protein isolate produced by the method of embodiment 165. 168. Crushed, non-viable seed of Brassica carinata cultivar AG 044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123014.
169. A cell of a seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123014.
170. A cell of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed defined in embodiment 169.
171. A protoplast of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed defined in embodiment 169.
172. The cell of embodiment 170, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, or a stalk.
173. The cell of embodiment 172, wherein the plant part is pollen.
174. The cell of embodiment 172, wherein the plant part is an ovule.
175. A cell of a Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of the plant defined in embodiment 170 when grown in the same location under the same environmental conditions.
176. A tissue culture of protoplasts or regenerable cells of the plant, or part thereof, defined in embodiment 170.
177. The tissue culture according to embodiment 176, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks.
178. A cell of a Brassica carinata plant regenerated from the tissue culture of embodiment 176 or 177, wherein said plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
179. A cell of a regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from the plant or part thereof defined in embodiment 170. 180. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce seed, wherein the seed is produced by self-fertilization or cross-fertilization.
181. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014 to produce an Fl hybrid Brassica carinata seed, wherein the plant is either a female parent or a male parent in a cross-fertilization.
182. The use of embodiment 181 wherein the plant is the female parent.
183. The use of embodiment 181 wherein the plant is the male parent.
184. A cell of an Fl hybrid plant grown from the Fl hybrid seed produced by the use according to any one of embodiments 181 to 183.
185. A cell of an Fl hybrid plant grown from Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed.
186. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Doubled Haploidy variety.
187. Use of embodiment 186, wherein chromosome doubling is introduced by chemical or physical means.
188. A cell of a Doubled Haploidy variety produced from Brassica carinata cultivar AGR044- 3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
189. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait.
190. The use of embodiment 189, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
191. The use of embodiment 190, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba. 192. A cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AG 044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of:
(a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and
(d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22.
193. The cell of embodiment 192, wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
194. The cell of embodiment 192 or 193, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
195. The cell of embodiment 194, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
196. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct.
197. The use of embodiment 196, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
198. The use of embodiment 196 or 197, wherein the DNA construct comprises a transgene.
199. The use of embodiment 196 or 197, wherein the DNA construct comprises an RNAi construct. 200. The use of any one of embodiments 196 to 199, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AG 044-3A22 when grown in the same location under the same environmental conditions.
201. The use of any one of embodiments 196 to 200, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
202. The use of embodiment 201, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
203. A cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22.
204. The cell of embodiment 203, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
205. The cell of embodiment 203 or 204, wherein the DNA construct comprises a transgene.
206. The cell of embodiment 203 or 204, wherein the DNA construct comprises an RNAi construct.
207. The cell of any one of embodiments 203 to 206, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
208. The cell of any one of embodiments 203 to 207, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
209. The cell of embodiment 208, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
210. A cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of:
(a) crossing a plant of cultivar AG 044-3A22 with a plant of another Brassica carinata variety comprising the desired trait;
(b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait;
(c) selfing the progeny plants that have the desired trait to produce further progeny plants; and
(d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22.
211. The cell of embodiment 210, wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
212. The cell of embodiment 210 or 211, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
213. The cell of embodiment 212, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
214. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a new trait, wherein the new trait is introduced by exposing seedlings or microspores to a mutagenic agent.
215. The use of embodiment 214, wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, or x-ray, gamma or ultraviolet radiation.
216. A cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a new trait, and wherein the Brassica carinata variety is produced by a method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
217. The cell of embodiment 216, wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, or x-ray, gamma or ultraviolet radiation.
218. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AG 044-3A22 with a plant of another Brassicaceae species comprising the desired trait.
219. The use of embodiment 218, wherein the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
220. The use of embodiment 218 or 219, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
221. The use of embodiment 220, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
222. A cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising:
(a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait;
(b) using embryo rescue techniques to recover viable Fl plants from the cross or
growing Fl seeds to produce Fl plants;
(c) selfing the Fl plants that have the desired trait and carinata character;
(d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants;
(e) selfing the F2 plants that have the desired trait and carinata character;
(f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants;
(g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and
(h) selecting the progeny plants with the desired trait and carinata character to
produce the carinata variety produced from cultivar AGR044-3A22.
223. The cell of embodiment 222, wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
224. The method of embodiment 222 or 223, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism. 225. The method of embodiment 224, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
226. Use of a plant of Brassica carinata cultivar AG 044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop.
227. Use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial plant product.
228. The use of embodiment 227, wherein the commercial plant product comprises oil, meal, or protein isolate.
229. A plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
230. The plant part of embodiment 229, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
231. A seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
232. A cell of a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
233. The cell of embodiment 232, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
234. A cell of a seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014. 4. EVALUATION CRITERIA [0038] In the description and tables which follow a number of terms are used. In order to aid in a clear and consistent understanding of the specification the following definitions and/or explanations of the assessment of the evaluation criteria are provided.
[0039] Type. Type refers to whether the new cultivar is considered to be primarily a Spring or Winter type of Brassica carinata.
[0040] Ploidy. Ploidy refers to whether the number of chromosomes exhibited by the cultivar is diploid or tetraploid.
[0041] Leaf Anthocyanin Coloration. The presence or absence of leaf anthocyanin coloration and the degree thereof if present are observed when the plant has reached the 9 to 11 leaf-stage.
[0042] Time of Flowering. A determination is made of the number of days when at least 50 percent of the plants have one or more open buds on a terminal raceme in the year of sowing.
[0043] Plant Height. The overall plant height at the end of flowering is observed (mean of 50).
[0044] Flower Petal Coloration. The coloration of open exposed petals on the first day of flowering is observed.
[0045] Pod Anthocyanin Coloration. The presence or absence at maturity of silique anthocyanin coloration, and the degree thereof if present are observed.
[0046] Silique attitude (Pod Habit). The typical manner in which the silique are borne on the plant at maturity is observed.
[0047] Maturity. The number of days from planting to maturity is observed with maturity being defined as the plant stage when pods with seed color change, occurring from green to brown or black, on the bottom third of the pod bearing area of the main stem.
[0048] Seeds Per Pod. The average number of seeds per pod is observed (mean of 50).
[0049] Seed Size. The weight in grams of 1,000 typical seeds is determined at maturity while such seeds exhibit a moisture content of approximately 5 to 6 percent by weight.
[0050] Seed Coat Color. The seed coat color of typical mature seeds is observed.
[0051] NIR analysis: Near infrared spectroscopy screening is a non-destructive method that allows one to determine the NIR absorption spectra of intact samples of material such as plant seed. Once the absorption spectra of the sample is determined, it can be used to determine the sample's content of compounds such as protein, oil, fatty acids, glucosinolates and others. This is done by comparing the spectra to those of calibrated samples. Calibration was developed in-house by correlating the NIR spectra with experimentally-measured seed quality parameters of over 700 diverse carinata lines. These lines comprise a highly-diverse range of oil contents, profiles, glucosinolate levels, seed colours, etc. and have been collected from regions all around the world. [0052] Oil Content: The typical percentage by weight oil present in the mature whole dried seeds is determined by Near Infrared Spectroscopy (NIR).
[0053] Protein Content: The typical percentage by weight of protein in the oil free meal of the mature whole dried seeds is determined by NIR analysis.
[0054] Fatty Acid Content: The typical percentages by weight of fatty acids present in the
endogenously formed oil of the mature whole dried seeds are determined by NIR analysis. Seed oil of Brassica carinata and other brassica oilseeds, are comprised largely of mono and poly unsaturated fatty acids ranging from 18 to 22 carbons in chain length Of the monosunsaturated fatty acids, one finds predominantly oleic acid, (cl8.1) , gondoic acid (C20.1) erucic acid (C22.1). Of the poly uncaturated fatty acids, one finds predominantly Linoleiic (C18.2) and Linolenic (cl8.3). The remaining fraction is comprised of saturated fatty acids (SATS), including Laurie (C12.0), Myristic acid (C14.0), Palmitic acid (C16) and Stearic acid (C18.0)
[0055] Glucosinolate Content. The total aliphatic glucosinolate content of the meal of the seeds is determined by NIR and is expressed as micromoles per gram seed weight.
[0056] Resistance to Shattering. Resistance to silique shattering is observed at seed maturity and is expressed on a scale of 1 (poor) to 5 (excellent).
[0057] Resistant to Lodging. Resistance to lodging at maturity and is expressed on a scale of 1 (weak) to 5 (strong).
[0058] Frost Tolerance (Spring Type Only). The ability of young plants to withstand late spring frosts at a typical growing area is evaluated and is expressed on a scale of 1 (poor) to 5 (excellent).
[0059] Disease Resistance: Resistant to various diseases is evaluated and is expressed on a scale of 0 (not tested), 1 (susceptible), 2 (low resistance), 3 (moderate resistance), or 4 (high resistance).
[0060] Herbicide Resistance: Resistance to various herbicides when applied at standard recommended application rates is expressed on a scale of 1 (resistant), 2 (tolerant), or 3(susceptible).
[0061] 5. DETAILED DESCRIPTION
[0062] AGR044-312D and AGR044-3A22 are inbred Brassica carinata varieties that has been selected on the basis of improved yield relative to existing commercial carinata varieties (eg A110 and A120). Other improvements relative to existing commercial carinata varieties may include: reduced levels of seed glucosinolate, improved disease resistance, reduced tendency to lodging and pod shatter.
[0063] In some embodiments, the present invention provides methods, uses, and compositions of matter related to the cells, seeds, plants or parts thereof, derivative seeds, and derivative plants of Brassica carinata cultivars AGR044-312D and AGR044-3A22. [0064] In the embodiments described below, "essentially all of the physiological and morphological characteristics" can mean, for example, the quantitative physiological and morphological characteristics described in Tables 1 and 3-7 for AGR044-3A22 or in Tables 1, 2, and 14 for AGR044-312D when grown in the same location under the same environmental conditions, as determined at the 5% significance level.
[0065] In the embodiments described below, "essentially all of the physiological and morphological characteristics" can also mean the quantitative physiological and morphological characteristics described in Tables 1, 3, and 5-7 for AGR044-3A22 or in Tables 1, 2, 5-10, and 12-14 for AGR044-312D when grown in the same location under the same environmental conditions, as determined at the 5% significance level.
[0066] 5.1 AGR044-312D
[0067] 5.1.1 Seeds, plants, plant parts and cells
[0068] In one embodiment, the invention provides a seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0069] In another embodiment, the invention provides a plant of Brassica carinata cultivar AGR044- 312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA- 123015.
[0070] In another embodiment, the invention provides a plant part of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
[0071] In another embodiment, the invention provides a plant part of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the plant part is pollen.
[0072] In another embodiment, the invention provides a plant part of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the plant part is an ovule.
[0073] In another embodiment, the invention provides a Brassica carinata plant, or a part thereof, having essentially all of the physiological and morphological characteristics of a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, when grown in the same location under the same environmental conditions.
[0074] In another embodiment, the invention provides a cell of a seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0075] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0076] In another embodiment, the invention provides a protoplast of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0077] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, or a stalk.
[0078] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the plant part is pollen.
[0079] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the plant part is an ovule.
[0080] In another embodiment, the invention provides a cell of a Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of a plant of Brassica carinata cultivar AGR044-312D produced from a seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, when grown in the same location under the same environmental conditions.
[0081] 5.1.2 Tissue cultures and regenerated plants
[0082] In another embodiment, the invention provides a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0083] In another embodiment, the invention provides a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks.
[0084] In another embodiment, the invention provides a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, when grown in the same location under the same environmental conditions.
[0085] In another embodiment, the invention provides a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, when grown in the same location under the same environmental conditions.
[0086] In another embodiment, the invention provides a regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of the cultivar AGR044-312D when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0087] In another embodiment, the invention provides a cell of a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044- 312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA- 123015, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA- 123015, when grown in the same location under the same environmental conditions. [0088] In another embodiment, the invention provides a cell of a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044- 312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA- 123015, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044- 312D, the seed of which has been deposited under ATCC Accession number PTA-123015, when grown in the same location under the same environmental conditions.
[0089] In another embodiment, the invention provides a cell of a regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of the cultivar AGR044-312D when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from a plant of Brassica carinata cultivar AGR044-312D, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0090] 5.1.3 Methods of crossing and uses for crossing Brassica carinata plants, and the cells and seeds produced therefrom
[0091] In another embodiment, the invention provides a method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0092] In another embodiment, the invention provides a Brassica carinata seed produced by a method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0093] In another embodiment, the invention provides a cell of a Brassica carinata seed produced by a method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0094] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce seed, wherein the seed is produced by self-fertilization or cross- fertilization.
[0095] 5.1.4 Methods of and uses for producing an Fl hybrid Brassica carinata seed, and the cells, seeds and plants produced therefrom
[0096] In another embodiment, the invention provides a method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing a plant of Brassica carinata cultivar AG 044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is either a female parent or a male parent.
[0097] In another embodiment, the invention provides a method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the female parent.
[0098] In another embodiment, the invention provides a method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the male parent.
[0099] In another embodiment, the invention provides an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is either a female parent or a male parent.
[0100] In another embodiment, the invention provides an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AG 044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the female parent.
[0101] In another embodiment, the invention provides an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the male parent.
[0102] In another embodiment, the invention provides an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is either a female parent or a male parent.
[0103] In another embodiment, the invention provides an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the female parent.
[0104] In another embodiment, the invention provides an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the male parent.
[0105] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015 to produce an Fl hybrid Brassica carinata seed, wherein the plant is either a female parent or a male parent in a cross-fertilization.
[0106] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015 to produce an Fl hybrid Brassica carinata seed, wherein the plant is the female parent. [0107] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015 to produce an Fl hybrid Brassica carinata seed, wherein the plant is the male parent.
[0108] In another embodiment, the invention provides a cell of an Fl hybrid plant grown from the Fl hybrid seed produced by any of the above uses.
[0109] In another embodiment, the invention provides a cell of an Fl hybrid plant grown from Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044- 312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed.
[0110] 5.1.5 Methods of and uses for producing a Doubled Haploidy variety, and the cells, seeds, and plants produced therefrom
[0111] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is either a female parent or a male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number.
[0112] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the female parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number. [0113] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number.
[0114] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is either a female parent or a male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number; wherein the method further comprises inducing chromosome doubling by chemical or physical means.
[0115] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D is the female parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number; wherein the method further comprises inducing chromosome doubling by chemical or physical means.
[0116] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AG 044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated
AGR044-312D is the male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number; wherein the method further comprises inducing chromosome doubling by chemical or physical means.
[0117] In another embodiment, the invention provides a plant, or part thereof, or seed of a Doubled Haploidy variety produced by any of the above methods.
[0118] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015 to produce a Doubled Haploidy variety.
[0119] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015 to produce a Doubled Haploidy variety, wherein chromosome doubling is introduced by chemical or physical means.
[0120] In another embodiment, the invention provides a cell of a Doubled Haploidy variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015.
[0121] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Doubled Haploidy variety.
[0122] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Doubled Haploidy variety, wherein chromosome doubling is introduced by chemical or physical means.
[0123] 5.1.6 Desired traits
[0124] In one aspect, the present invention includes the introduction of a desired trait into Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising the desired trait. [0125] Examples of potential desired traits include
a. cytoplasmic male sterility, CMS restorer traits,
b. biotic and abiotic stress resistance such as disease resistance, fungal resistance, pest resistance, drought tolerance, and frost tolerance,
c. agronomic traits such as increased pod shatter resistance, improved harvestability, improved nutrient usage efficiency,seed colour seed size, seed pod size, seed pod architecture, seed pod fill, earlier and more uniform time to flowering , earlier maturity, extent of branching, flower colour and density, and plant height,
d. altered metabolism (increased seed oil, increased seed protein, altered seed oil or fatty acid profile, reduced seed content of glucosinolates and other antinutritionals),
e. improved performance: improved oil per unit area, improved grain per unit area, f. herbicide tolerance including tolerance to glyphosate, glufosinate, imidazolinones and auxin analogues such as 2,4-D and dicamba.
[0126] 5.1.7 Methods of and uses for introducing a desired trait by crossing and backcrossing, and the cells, seeds and plants produced therefrom
[0127] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AG 044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
[0128] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AG 044-312D when grown in the same location under the same environmental conditions.
[0129] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0130] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions; and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0131] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AG 044-312D; and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, auxin analogues such as 2,4-D and dicamba.
[0132] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions; and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, auxin analogues such as 2,4-D and dicamba.
[0133] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any of the above methods.
[0134] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait.
[0135] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0136] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0137] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AG 044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
[0138] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123015, wherein the variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
[0139] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0140] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AG 044-312D; wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0141] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0142] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-312D to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0143] 5.1.8 DNA constructs [0144] In one aspect, the present invention includes the introduction of a desired trait into Brassica carinata cultivar AG 044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising the desired trait, wherein the desired trait is conferred by a DNA construct.
[0145] The DNA construct can be introduced by a variety of methods, including by using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated
microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
[0146] The DNA construct can comprise any type of DNA, including a transgene or a DNA construct that is designed to modulate the expression of endogenous genes.
[0147] Examples of transgenes that could be incorporated include, but are not limited to, the following group: Crambe abbysinica FAE1, Teesdalia nodulicans FAE1, Cardamine graeca FAE1 Brassica napus DGAT, Tropaeolum majus DGAT, Yeast SLC1
[0148] DNA constructs that are designed to modulate the expression of endogenous genes include, but are not limited to the following group: Brassica carinata Myb28, Myb29, FAD2 and FAD3 antisense RNA or RNAi sequences, which can be used to interfere or knock down the expression of endogenous genes to extremely low levels, simulating the effect of a null mutation at the endogenous locus. As discussed above, because Brassica carinata is amphidiploid, it can have multiple copies of genes from the contributing ancestral species that may create a high level of functional redundancy. As such, a single mutation in one of the homologues may not be sufficient to confer a phenotype. By using RNAi or an antisense approach, one may conceivably be capable of targeting all of the expressed homologues and achieving a functional knockdown effect. Such approaches require the RNAi or antisense RNA to be stably expressed.
[0149] 5.1.9 Methods of and uses for introducing a desired trait using DNA constructs, and the cells, seeds and plants produced therefrom
[0150] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D.
[0151] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, and wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector. [0152] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AG 044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, and wherein the DNA construct comprises a transgene.
[0153] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises a transgene.
[0154] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, and wherein the DNA construct comprises an RNAi construct.
[0155] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises an RNAi construct.
[0156] In another embodiment, the invention provides any one of the above methods, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D, when grown in the same location under the same environmental conditions.
[0157] In another embodiment, the invention provides any one of the above methods, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0158] In another embodiment, the invention provides any one of the above methods, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0159] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any one of the above methods.
[0160] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AG 044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct.
[0161] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
[0162] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, and wherein the DNA construct comprises a transgene.
[0163] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises a transgene.
[0164] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, and wherein the DNA construct comprises an RNAi construct.
[0165] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises an RNAi construct. [0166] In another embodiment, the invention provides any of the above uses, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AG 044-312D when grown in the same location under the same
environmental conditions.
[0167] In another embodiment, the invention provides any of the above uses, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0168] In another embodiment, the invention provides any of the above uses, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0169] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D.
[0170] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
[0171] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, and wherein the DNA construct comprises a transgene.
[0172] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises a transgene. [0173] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AG 044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, and wherein the DNA construct comprises an RNAi construct.
[0174] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-312D, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises an RNAi construct.
[0175] In another embodiment, the invention provides any of the above cells, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same
environmental conditions.
[0176] In another embodiment, the invention provides any of the above cells, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0177] In another embodiment, the invention provides any of the above cells, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0178] 5.1.10 Methods of and uses for introducing a desired trait by an initial cross and then pedigree selection, and cells, plants and seeds produced therefrom
[0179] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny seed; and (d) growing the further progeny seed and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D. [0180] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AG 044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny seed; and (d) growing the further progeny seed and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
[0181] In another embodiment, the invention provides any one of the above methods wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0182] In another embodiment, the invention provides any one of the above methods, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0183] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any one of the above methods.
[0184] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D.
[0185] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044- 312D when grown in the same location under the same environmental conditions.
[0186] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0187] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044- 312D when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0188] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba. [0189] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AG 044-312D, the seed of which has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-312D; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044- 312D when grown in the same location under the same environmental conditions; and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0190] 5.1.11 Mutagenesis
[0191] In one aspect, the present invention includes the introduction of a desired trait into Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising the desired trait, wherein the desired trait is introduced by mutagenesis.
[0192] Any means of mutagenesis can potentially be used, including the mutagenic agents ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0193] The mutagenization can be of a variety of parts of the plants, including a seed, seedling, or microspore. Mutagenized microspores can then be used to generate doubled haploid plants (see above). Seedlings or microspores are exposed to the mutagenic agent and then the surviving fraction are allowed to develop into mature plants. In some cases, the mutagenized plantlets or embryos (in the case of microspore mutagenesis) may be exposed to selection in order to enrich for a particular phenotype. This technique can be used to develop varieties with a desired trait, such as resistance to a herbicide, an altered seed oil profile, increased tolerance to disease, or abiotic stress.
[0194] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants. [0195] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AG 044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the Brassica carinata variety comprises a desired trait, the method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants, and wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0196] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any of the above methods.
[0197] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is introduced by exposing seedlings or microspores to a mutagenic agent.
[0198] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is introduced by exposing seedlings or microspores to a mutagenic agent, and wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0199] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
[0200] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants, wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0201] 5.1.12 Methods of or uses for producing a carinata variety by outcrossing (interspecific or wide crossing), and cells, plants, seeds produced therefrom [0202] Where no Brassica carinata variety has a specific desired trait, outcrossing (interspecific or wide crossing) can be used where the trait is found in another Brassicaceae species, such as, for example, Brassica napus, Brassica juncea, Brassica oleracea, Brassica rapa, or Brassica nigra.
[0203] In another embodiment, the invention provides a method of producing a carinata variety produced from Brassica carinata cultivar AG 044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the carinata variety comprises a desired trait, the method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h)selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044- 312D.
[0204] In another embodiment, the invention provides a method of producing a carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, and wherein the carinata variety comprises a desired trait, the method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h)selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044- 312D; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044- 312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
[0205] In another embodiment, the invention provides any of the above methods, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0206] In another embodiment, the invention provides any of the above methods, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba. [0207] In another embodiment, the invention provides any of the above methods, wherein the method further comprises producing a doubled haploidy variety from the carinata variety.
[0208] In another embodiment, the invention provides a plant, or part thereof, or seed of a carinata variety produced by any of the above methods.
[0209] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait.
[0210] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait, and wherein the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
[0211] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0212] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait, and wherein the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0213] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait, and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0214] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait, and wherein the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0215] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-312D.
[0216] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-312D; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions.
[0217] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AG 044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-312D; wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0218] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-312D; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0219] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-312D; wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba. [0220] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-312D with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-312D; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-312D has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-312D when grown in the same location under the same environmental conditions, and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0221] 5.1.13 Commercial crops and commercial plant products
[0222] In another embodiment, the invention provides a method of producing a commercial plant product, the method comprising growing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop, and producing said commercial plant product from the commercial crop.
[0223] In another embodiment, the invention provides a method of producing a commercial plant product, the method comprising growing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop, and producing said commercial plant product from the commercial crop, wherein the commercial plant product comprises oil, meal or protein isolate.
[0224] In another embodiment, the invention provides a method of producing a commercial plant product, the method comprising growing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop, and producing said commercial plant product from the commercial crop, wherein the commercial plant product comprises a biofumigant.
[0225] In another embodiment, the invention provides a commercial plant product produced by a method comprising growing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop, and producing said commercial plant product from the commercial crop.
[0226] In another embodiment, the invention provides a commercial plant product produced by a method comprising growing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop, and producing said commercial plant product from the commercial crop, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0227] In another embodiment, the invention provides oil, meal, or protein isolate produced by a method comprising growing a plant of Brassica carinata cultivar AGR044-312D produced from the seed of Brassica carinata cultivar designated AGR044-312D, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop, and producing the oil, meal, or protein isolate from the commercial crop.
[0228] In another embodiment, the invention provides crushed, non-viable seed of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0229] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop.
[0230] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial plant product.
[0231] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial plant product, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0232] In another embodiment, the invention provides crushed, non-viable seed of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0233] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial crop.
[0234] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial plant product. [0235] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, to produce a commercial plant product, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0236] 5.2 AGR044-3A22
[0237] 5.2.1 Seeds, plants, plant parts and cells
[0238] In one embodiment, the invention provides a seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0239] In another embodiment, the invention provides a plant of Brassica carinata cultivar AGR044- 3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA- 123014.
[0240] In another embodiment, the invention provides a plant part of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
[0241] In another embodiment, the invention provides a plant part of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the plant part is pollen.
[0242] In another embodiment, the invention provides a plant part of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the plant part is an ovule.
[0243] In another embodiment, the invention provides a Brassica carinata plant, or a part thereof, having essentially all of the physiological and morphological characteristics of a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, when grown in the same location under the same environmental conditions.
[0244] In another embodiment, the invention provides a cell of a seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014. [0245] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
[0246] In another embodiment, the invention provides a protoplast of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
[0247] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, or a stalk.
[0248] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the plant part is pollen.
[0249] In another embodiment, the invention provides a cell of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from a seed of Brassica carinata cultivar designated AGR044- 3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the plant part is an ovule.
[0250] In another embodiment, the invention provides a cell of a Brassica carinata plant, or parts thereof, having essentially all of the physiological and morphological characteristics of a plant of Brassica carinata cultivar AGR044-3A22 produced from a seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, when grown in the same location under the same environmental conditions.
[0251] 5.2.2 Tissue cultures and regenerated plants
[0252] In another embodiment, the invention provides a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0253] In another embodiment, the invention provides a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks.
[0254] In another embodiment, the invention provides a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, when grown in the same location under the same environmental conditions.
[0255] In another embodiment, the invention provides a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, when grown in the same location under the same environmental conditions.
[0256] In another embodiment, the invention provides a regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of the cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0257] In another embodiment, the invention provides a cell of a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044- 3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA- 123014, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA- 123014, when grown in the same location under the same environmental conditions.
[0258] In another embodiment, the invention provides a cell of a Brassica carinata plant regenerated from a tissue culture of protoplasts or regenerable cells of a plant of Brassica carinata cultivar AGR044- 3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA- 123014, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks, wherein the plant has essentially all of the morphological and physiological characteristics of cultivar AGR044- 3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, when grown in the same location under the same environmental conditions.
[0259] In another embodiment, the invention provides a cell of a regenerated Brassica carinata plant having essentially all of the physiological and morphological characteristics of the cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, the regenerated plant having been produced using a tissue culture, wherein the tissue culture is produced from a plant of Brassica carinata cultivar AGR044-3A22, or a part thereof, produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0260] 5.2.3 Methods of crossing and uses for crossing Brassica carinata plants, and the cells and seeds produced therefrom
[0261] In another embodiment, the invention provides a method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0262] In another embodiment, the invention provides a Brassica carinata seed produced by a method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0263] In another embodiment, the invention provides a cell of a Brassica carinata seed produced by a method for producing Brassica carinata seed comprising crossing Brassica carinata plants and harvesting the resulting Brassica carinata seed, wherein at least one Brassica carinata plant is a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0264] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce seed, wherein the seed is produced by self-fertilization or cross- fertilization.
[0265] 5.2.4 Methods of and uses for producing an Fl hybrid Brassica carinata seed, and the cells, seeds and plants produced therefrom [0266] In another embodiment, the invention provides a method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing a plant of Brassica carinata cultivar AG 044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is either a female parent or a male parent.
[0267] In another embodiment, the invention provides a method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the female parent.
[0268] In another embodiment, the invention provides a method for producing a first generation (Fl) hybrid Brassica carinata seed comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the male parent.
[0269] In another embodiment, the invention provides an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is either a female parent or a male parent.
[0270] In another embodiment, the invention provides an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the female parent.
[0271] In another embodiment, the invention provides an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the male parent.
[0272] In another embodiment, the invention provides an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is either a female parent or a male parent.
[0273] In another embodiment, the invention provides an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the female parent.
[0274] In another embodiment, the invention provides an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a
representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the male parent.
[0275] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014 to produce an Fl hybrid Brassica carinata seed, wherein the plant is either a female parent or a male parent in a cross-fertilization.
[0276] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014 to produce an Fl hybrid Brassica carinata seed, wherein the plant is the female parent.
[0277] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014 to produce an Fl hybrid Brassica carinata seed, wherein the plant is the male parent. [0278] In another embodiment, the invention provides a cell of an Fl hybrid plant grown from the Fl hybrid seed produced by any of the above uses.
[0279] In another embodiment, the invention provides a cell of an Fl hybrid plant grown from Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044- 3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed.
[0280] 5.2.5 Methods of and uses for producing a Doubled Haploidy variety, and the cells, seeds, and plants produced therefrom
[0281] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is either a female parent or a male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number.
[0282] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the female parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number.
[0283] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number.
[0284] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is either a female parent or a male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number; wherein the method further comprises inducing chromosome doubling by chemical or physical means.
[0285] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the female parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number; wherein the method further comprises inducing chromosome doubling by chemical or physical means.
[0286] In another embodiment, the invention provides a method for producing a Doubled Haploidy variety comprising: (a) isolating a flower bud of an Fl hybrid plant grown from an Fl hybrid seed produced by a method comprising crossing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, with a different Brassica carinata plant and harvesting the resultant Fl hybrid carinata seed, and wherein the plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22 is the male parent; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occured through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number; wherein the method further comprises inducing chromosome doubling by chemical or physical means.
[0287] In another embodiment, the invention provides a plant, or part thereof, or seed of a Doubled Haploidy variety produced by any of the above methods.
[0288] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AG 044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014 to produce a Doubled Haploidy variety.
[0289] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014 to produce a Doubled Haploidy variety, wherein chromosome doubling is introduced by chemical or physical means.
[0290] In another embodiment, the invention provides a cell of a Doubled Haploidy variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014.
[0291] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Doubled Haploidy variety.
[0292] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Doubled Haploidy variety, wherein chromosome doubling is introduced by chemical or physical means.
[0293] 5.2.6 Desired traits
[0294] In one aspect, the present invention includes the introduction of a desired trait into Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising the desired trait.
[0295] Examples of potential desired traits include:
a. cytoplasmic male sterility, CMS restorer traits,
b. biotic and abiotic stress resistance such as disease resistance, fungal resistance, pest resistance, drought tolerance, and frost tolerance,
c. agronomic traits such as increased pod shatter resistance, improved harvestability, improved nutrient usage efficiency,seed colour seed size, seed pod size, seed pod architecture, seed pod fill, earlier and more uniform time to flowering , earlier maturity, extent of branching, flower colour and density, and plant height,
d. altered metabolism (increased seed oil, increased seed protein, altered seed oil or fatty acid profile, reduced seed content of glucosinolates and other antinutritionals),
e. improved performance: improved oil per unit area, improved grain per unit area, f. herbicide tolerance including tolerance to glyphosate, glufosinate, imidazolinones and auxin analogues such as 2,4-D and dicamba.
[0296] 5.2.7 Methods of and uses for introducing a desired trait by crossing and backcrossing, and the cells, seeds and plants produced therefrom
[0297] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22.
[0298] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
[0299] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0300] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions; and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0301] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0302] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044- 3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny seed; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions; and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0303] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any of the above methods.
[0304] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait.
[0305] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0306] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0307] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22. [0308] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123014, wherein the variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
[0309] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0310] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism. [0311] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AG 044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0312] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession numberPTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) backcrossing the selected progeny plants that have the desired trait with plants of cultivar AGR044-3A22 to produce backcross progeny plants; and (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein the method to produce the Brassica carinata variety further comprises repeating steps (c) and (d) until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0313] 5.2.8 DNA constructs
[0314] In one aspect, the present invention includes the introduction of a desired trait into Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising the desired trait, wherein the desired trait is conferred by a DNA construct.
[0315] The DNA construct can be introduced by a variety of methods, including by using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated
microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector. [0316] The DNA construct can comprise any type of DNA, including a transgene or a DNA construct that is designed to modulate the expression of endogenous genes.
[0317] Examples of transgenes that could be incorporated can include, but are not limited to, the following: Crambe abbysinica FAE1, Teesdalia nodulicans FAE1, Cardamine graeca FAE1 Brassica napus DGAT, Tropaeolum majus DGAT, Yeast SLC1
[0318] DNA constructs that are designed to modulate the expression of endogenous genes may include, but are not limited to the following group: Brassica carinata Myb28, Myb29, FAD2 and FAD3 antisense NA or RNAi sequences, which can be used to interfere or knock down the expression of endogenous genes to extremely low levels, simulating the effect of a null mutation at the endogenous locus. As discussed above, because Brassica carinata is amphidiploid, it can have multiple copies of genes from the contributing ancestral species that may create a high level of functional redundancy. As such, a single mutation in one of the homologues may not be sufficient to confer a phenotype. By using RNAi or an antisense approach, one may conceivably be capable of targeting all of the expressed homologues and achieving a functional knockdown effect. Such approaches require the RNAi or antisense RNA to be stably expressed.
[0319] 5.2.9 Methods of and uses for introducing a desired trait using DNA constructs, and the cells, seeds and plants produced therefrom
[0320] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22.
[0321] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, and wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
[0322] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, and wherein the DNA construct comprises a transgene. [0323] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AG 044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises a transgene.
[0324] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, and wherein the DNA construct comprises an RNAi construct.
[0325] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, the method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises an RNAi construct.
[0326] In another embodiment, the invention provides any one of the above methods, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22, when grown in the same location under the same environmental conditions.
[0327] In another embodiment, the invention provides any one of the above methods, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0328] In another embodiment, the invention provides any one of the above methods, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0329] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any one of the above methods.
[0330] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct.
[0331] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
[0332] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, and wherein the DNA construct comprises a transgene.
[0333] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises a transgene.
[0334] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, and wherein the DNA construct comprises an RNAi construct.
[0335] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is conferred by a DNA construct, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises an RNAi construct.
[0336] In another embodiment, the invention provides any of the above uses, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same
environmental conditions. [0337] In another embodiment, the invention provides any of the above uses, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0338] In another embodiment, the invention provides any of the above uses, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0339] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AG 044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22.
[0340] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector.
[0341] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, and wherein the DNA construct comprises a transgene.
[0342] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises a transgene.
[0343] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AG 044-3A22, and wherein the DNA construct comprises an RNAi construct.
[0344] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising introducing a DNA construct conferring the desired trait into a plant of cultivar AGR044-3A22, wherein the DNA construct is introduced using polyethylene glycol (PEG) mediated DNA uptake, electroporation, ballistic infiltration using DNA coated microprojectiles (gene gun), an Agrobacteria infiltration based vector, or a plant virus based vector, and wherein the DNA construct comprises an RNAi construct.
[0345] In another embodiment, the invention provides any of the above cells, wherein the Brassica carinata variety comprises the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same
environmental conditions.
[0346] In another embodiment, the invention provides any of the above cells, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0347] In another embodiment, the invention provides any of the above cells, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0348] 5.2.10 Methods of and uses for introducing a desired trait by an initial cross and then pedigree selection, and cells, plants and seeds produced therefrom
[0349] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny seed; and (d) growing the further progeny seed and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22.
[0350] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny seed; and (d) growing the further progeny seed and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
[0351] In another embodiment, the invention provides any one of the above methods wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0352] In another embodiment, the invention provides any one of the above methods, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0353] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any one of the above methods.
[0354] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22.
[0355] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044- 3A22 when grown in the same location under the same environmental conditions. [0356] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0357] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044- 3A22 when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0358] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0359] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, the seed of which has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety was produced by a method comprising the steps of: (a) crossing a plant of cultivar AG 044-3A22 with a plant of another Brassica carinata variety comprising the desired trait; (b) growing the resultant Fl hybrid seed and selecting one or more progeny plants that have the desired trait; (c) selfing the progeny plants that have the desired trait to produce further progeny plants; and (d) growing the resultant further progeny plants and selecting further progeny plants that have the desired trait to produce the Brassica carinata variety produced from cultivar AGR044-3A22; wherein steps (c) and (d) are repeated until the Brassica carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044- 3A22 when grown in the same location under the same environmental conditions; and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0360] 5.2.11 Mutagenesis
[0361] In one aspect, the present invention includes the introduction of a desired trait into Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising the desired trait, wherein the desired trait is introduced by mutagenesis.
[0362] Any means of mutagenesis can potentially be used, including the mutagenic agents ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0363] The mutagenization can be of a variety of parts of the plants, including a seed, seedling, or microspore. Mutagenized microspores can then be used to generate doubled haploid plants (see above). Seedlings or microspores are exposed to the mutagenic agent and then the surviving fraction are allowed to develop into mature plants. In some cases, the mutagenized plantlets or embryos (in the case of microspore mutagenesis) may be exposed to selection in order to enrich for a particular phenotype. This technique can be used to develop varieties with a desired trait, such as resistance to a herbicide, an altered seed oil profile, increased tolerance to disease, or abiotic stress.
[0364] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
[0365] In another embodiment, the invention provides a method of producing a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the Brassica carinata variety comprises a desired trait, the method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants, and wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0366] In another embodiment, the invention provides a plant, or part thereof, or seed of a Brassica carinata variety produced by any of the above methods.
[0367] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is introduced by exposing seedlings or microspores to a mutagenic agent.
[0368] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a Brassica carinata variety comprising a desired trait, wherein the desired trait is introduced by exposing seedlings or microspores to a mutagenic agent, and wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0369] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants.
[0370] In another embodiment, the invention provides a cell of a plant of a Brassica carinata variety produced from cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, wherein the Brassica carinata variety comprises a desired trait, and wherein the Brassica carinata variety is produced by a method comprising exposing seedlings or microspores to a mutagenic agent and allowing the surviving fraction to develop into mature plants, wherein the mutagenic agent is ethyl methanesulfonate, N-ethyl-N-nitrosourea, ionizing radiation such as x-ray or gamma, or ultraviolet radiation.
[0371] 5.2.12 Methods of or uses for producing a carinata variety by outcrossing (interspecific or wide crossing), and cells, plants, seeds produced therefrom
[0372] Where no Brassica carinata variety has a specific desired trait, outcrossing (interspecific or wide crossing) can be used where the trait is found in another Brassicaceae species, such as, for example, Brassica napus, Brassica juncea, Brassica oleracea, Brassica rapa, or Brassica nigra.
[0373] In another embodiment, the invention provides a method of producing a carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the carinata variety comprises a desired trait, the method comprising: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h)selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044- 3A22.
[0374] In another embodiment, the invention provides a method of producing a carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, and wherein the carinata variety comprises a desired trait, the method comprising: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h)selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044- 3A22; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044- 3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
[0375] In another embodiment, the invention provides any of the above methods, wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0376] In another embodiment, the invention provides any of the above methods, wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0377] In another embodiment, the invention provides any of the above methods, wherein the method further comprises producing a doubled haploidy variety from the carinata variety.
[0378] In another embodiment, the invention provides a plant, or part thereof, or seed of a carinata variety produced by any of the above methods.
[0379] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait.
[0380] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait, and wherein the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
[0381] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0382] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait, and wherein the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0383] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait, and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0384] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a carinata variety comprising a desired trait, wherein the desired trait is introduced by crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait, and wherein the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0385] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AG 044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-3A22.
[0386] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-3A22; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions.
[0387] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-3A22; wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism. [0388] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AG 044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-3A22; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, and wherein the desired trait is selected from the group consisting of male sterility, disease resistance, fungal resistance, pest resistance, herbicide tolerance, abiotic stress tolerance, and altered metabolism.
[0389] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-3A22; wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0390] In another embodiment, the invention provides a cell of a plant of a carinata variety comprising a desired trait, wherein the carinata variety is produced by a method comprising: (a) crossing a plant of cultivar AGR044-3A22 with a plant of another Brassicaceae species comprising the desired trait; (b) using embryo rescue techniques to recover viable Fl plants from the cross or growing Fl seeds to produce Fl plants; (c) selfing the Fl plants that have the desired trait and carinata character; (d) using embryo rescue techniques to recover viable F2 plants or growing F2 seeds to produce F2 plants; (e) selfing the F2 plants that have the desired trait and carinata character; (f) using embryo rescue techniques to recover viable F3 plants or growing F3 seeds to produce progeny plants; (g) selfing the progeny plants that have the desired trait and carinata character to produce further progeny plants; and (h) selecting the progeny plants with the desired trait and carinata character to produce the carinata variety produced from cultivar AGR044-3A22; wherein steps (g) and (h) are repeated until the carinata variety produced from cultivar AGR044-3A22 has the desired trait and essentially all of the physiological and morphological characteristics of cultivar AGR044-3A22 when grown in the same location under the same environmental conditions, and wherein the desired trait is herbicide tolerance and the tolerance is conferred to a herbicide selected from but not limited to the group consisting of glyphosate, glufosinate, imidazolinones, and auxin analogues such as 2,4-D and dicamba.
[0391] 5.2.13 Commercial crops and commercial plant products
[0392] In another embodiment, the invention provides a method of producing a commercial plant product, the method comprising growing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop, and producing said commercial plant product from the commercial crop.
[0393] In another embodiment, the invention provides a method of producing a commercial plant product, the method comprising growing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop, and producing said commercial plant product from the commercial crop, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0394] In another embodiment, the invention provides a method of producing a commercial plant product, the method comprising growing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop, and producing said commercial plant product from the commercial crop, wherein the commercial plant product comprises a biofumigant.
[0395] In another embodiment, the invention provides a commercial plant product produced by a method comprising growing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop, and producing said commercial plant product from the commercial crop.
[0396] In another embodiment, the invention provides a commercial plant product produced by a method comprising growing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop, and producing said commercial plant product from the commercial crop, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0397] In another embodiment, the invention provides oil, meal, or protein isolate produced by a method comprising growing a plant of Brassica carinata cultivar AGR044-3A22 produced from the seed of Brassica carinata cultivar designated AGR044-3A22, wherein a representative sample of the seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop, and producing the oil, meal, or protein isolate from the commercial crop.
[0398] In another embodiment, the invention provides crushed, non-viable seed of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
[0399] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop.
[0400] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial plant product.
[0401] In another embodiment, the invention provides use of a plant of Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial plant product, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0402] In another embodiment, the invention provides crushed, non-viable seed of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
[0403] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial crop.
[0404] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial plant product.
[0405] In another embodiment, the invention provides use of a plant of a Brassica carinata variety produced from Brassica carinata cultivar AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, to produce a commercial plant product, wherein the commercial plant product comprises oil, meal, or protein isolate.
[0406] 5.3 Characteristics of AGR044-312D and AGR044-3A22
[0407] Example 1: AGR044-312D and AGR044-3A22 yield performance in North Dakota , South Dakota and Saskatchewan
[0408] Yield trials were carried out for AGR044-312D at Winner, South Dakota; Hettinger, North Dakota; Moosomin, SK; and Vanguard, SK during the summer of 2015 in small plots using a three replicate randomized complete block design. These tests including 312D were designated "PYT A". This same experimental design was used for yield trials including AG 044-3A22, and data for two reliable sites was collected at Winner, South Dakota and Tioga, North Dakota. Yields were calculated from the mean of replicate plots of the test variety and expressed as a percentage of the mean yield of the check varieties in the same trial (A110 and A120, the current commercial varieties, were used as checks).
[0409] Table 1 records seed yield relative to the A110 check in yield testing in 2015. In the Hettinger trial, AGR044-312D yielded substantially higher than both of the commercial carinata check varieties; while in the Winner trial this variety yielded more than the A110 commercial check but not A120. In both Moosomin and Vanguard, AGR044-312D yielded lower than both A110 and A120. It should be noted that 312D has been tested as an improved variety for more southern geographies such as in the Southeast U.S. or South America. Therefore, yield data for AGR044-312D in these northern sites is not necessarily indicative of the full yield potential of this variety. For AGR044-3A22 in Tioga, yields substantially exceed both check lines, while at the Winner trial this variety yielded more than the A110 but not A120.
Table 1: AGR044-312D and -3A22 yields relative to checks in North Dakota, South Dakota and Saskatchewan yield trials (2015)
[0410] Example 2: AGR044-312D and AGR044-3A22 agronomic traits in North Dakota, South Dakota and Saskatchewan testing
[0411] Observations were made of various distinguishing traits leaf, flower and silique colouration, plant height, as well as agronomic traits such as days to flower (DTF), days to maturity DTM). Table 2 summarizes these observations for the AGR044-312D variety and Table 3 for the AGR044-3A22 variety. Table 2: AGR044- 312D Unique plant traits (North Dakota, South Dakota and Saskatchewan, 2015)
Table 3: AGR044-3A22 Unique plant traits (North Dakota, South Dakota and Saskatchewan, 2015)
[0412] Example 3: Seed quality characteristics of AGR044-312D and AGR044-3A22 in 2015 testing (North Dakota, South Dakota and Saskatchewan)
[0413] Seed samples from each plot in the 2015 preliminary trial sites were used for seed quality data analysis. Seed quality estimates were obtained for AGR044-312D at Winner, South Dakota; Moosomin, SK; and Vanguard, SK; and for AGR044-3A22 at Winner, SD and Tioga, ND [0414] Seed oil, GSL (glucosinolate) content, protein content and fatty acid profile were determined by NIR analysis on a FOSS XDS™ Rapid Content Analyser fitted with an auto sampling unit. For NIR analysis, a minimum of 5 g of seed sample (cleaned and dried to approximately 5% moisture) was placed in a ring cup, tracking code recorded, and sample spectra collected at 0.5 nm increments, over the range of 400- 2500 nm. Calibration was developed in-house by correlating the NIR spectra with experimentally- measured seed quality parameters of a diverse set of carinata experimental lines, representing as wide a spectrum of seed quality characteristics as could be found in this species (diverse range of oil contents, profiles, glucosinolate levels, seed colours, etc.). NIR values for oil content (% of whole seed) were validated using data obtained from NMR analysis of samples on an Oxford MARAN Ultra benchtop NM R system. NIR determination of seed Fatty-acid profile (and derived statistics, such as % SATS and % LCFA) was calibrated using Gas Chromatography of the Fatty-Acid Methyl Esters (FAMEs) using the protocol described by Taylor et al. (1992). NIR based glucosinolate determination was calibrated using Canadian Grains Commission data, a combination of the ISO 9167 method and their own NIR measurements. For calibration, seed samples were analyzed using our NIR instrument, the spectra collected and the experimental values recorded. The data set was then subjected to mathematical modeling to refine the spectra and the WinlSI software package was used to determine the spectral regions most predictive of the desired parameters. Points providing >99.99% correlation with the experimental values
(determined as described above) were selected from the spectral curve and used to develop the predictive equation.
[0415] Least square means of replicate seed oil and GSL content data were calculated and their standard errors compared via REM L analysis to determine whether seed quality differences between tested carinata varieties were significant. Values not sharing a group letter (Tables 4 and 5) were significantly different at P < 0.05, using the Student comparison of LS Mean method. All statistical analysis was performed using the JMP statistical analysis software (SAS)
[0416] Seed oil content: As can be seen in Table 4, the mean oil content of AGR044-312D was consistently 3 -4 % lower than those of the check varieties A120 and A110 in three groups of yield trials carried out in N. Dakota, S. Dakota and Saskatchewan during the summer of 2015. The variety AGR044- 3A22, was closer in oil content to the check lines. For this variety, comparison of the means using REML test showed no significant differences between AGR044-3A22, A120 and A110.
[0417] Glucosinolate content: As can be seen in Table 5, the varieties AGR044-312D and AGR044-3A22 clearly demonstrated a significantly lower seed GSL content relative to A110 and A120 seed. For AGR044-312D, decreases in GSL content of 18-23% relative to A110 and 19-28% relative to A120 were observed. For AGR044-3A22, decreases in GSL content of 27% relative to A110 and 32% relative to A120 were observed. Table 4: Seed quality versus check lines; oil content of AGR044-312D and AGR044-3A22 compared with check lines in 2015 yield testing (North Dakota, South Dakota and Saskatchewan, 2015)
Table 5: Seed quality versus check lines; seed Glucosinolate content of AGR044-312D and AGR044- 3A22 compared with check lines in 2015 yield testing (North Dakota, South Dakota and Saskatchewan)
[0418] Protein: Brassica carinata seed is known to contain appreciable levels of protein. When oil is extracted from the seed in commercial crushing operations, the remaining meal fraction can in itself be a valuable co-product, serving as a source of protein for use in animal feed applications. Therefore, in any assessment of new carinata varieties for commercial potential, seed protein content is an important consideration. Table 6 compares the protein contents of AGR044-312D and AGR044- 3A22 with those of check lines A110 and A120 in seed harvested from 2015 field trials. The least square means estimate of AGR044-312D and AGR044-3A22 whole seed protein values was 26 to 27%; and for the checks it was 28 to 29% (Table 6).
Table 6: Seed quality versus check lines; seed protein content of AGR044-312D and AGR044-3A22 compared with check lines in 2015 yield testing (North Dakota, South Dakota and Saskatchewan)
[0419] Fatty acid profile: The composition of carinata oil makes it suitable as an industrial feedstock in a number of biofuel manufacturing applications (see for example Gesch et al 2015, Wagid et al 2015). In particular, high levels of long and very long chain monounsaturated fatty acid content and a low proportion of saturated fatty acids are key definers of the current carinata profile and significant divergence from this profile in new carinata varieties would be problematic. Table 7 shows the fatty acid profile of AGR044-312D, AGR044-3A22 and the A110 and A120 check lines from grain harvested from 2015 trials carried out in North Dakota, South Dakota and Saskatchewan. As can be seen, the VLCFA erucic acid (C22.1) proportion in oil of both AGR044-312D and AGR044-3A22 falls very close to those of the commercial check lines in all trials. Similarly, the levels of saturated fatty acids in both AGR044-312D and AGR044-3A22 oil are close to those of the check varieties. Tthe composition of the other main fatty acid constituents of AGR044-312D and AGR044-3A22 also do not deviate substantially from those of their check line counterparts. This it is expected that the physical properties of the AGR044-312D and AGR044-3A22 oil that are dependant on fatty acid composition should not differ greatly from those of the check lines. Table 7: Fatty acid profile of oil from grain harvested in North Dakota, Soiuth Dakota and Saskatchewan trials (2015)
Site Name SATS SD C18.1 SD C18.2 SD C18.3 SD C20.1 SD C22.1 SD
Moosomin,
AAC A110 6.03 0.18 8.13 0.95 14.97 0.64 14.67 0.27 8.75 0.59 40.39 0.64 SK
Moosomin,
AAC A120 6.27 0.13 8.25 0.72 15.69 0.78 13.99 0.36 9.12 0.35 38.55 1.07 SK
Moosomin,
AGR044-312D 6.11 0.08 10.14 1.21 16.31 0.30 12.91 0.14 9.42 0.26 41.46 0.70
SK
Vanguard,
AAC A110 6.41 0.24 13.83 1.21 16.09 1.12 13.39 0.67 10.04 0.28 34.90 2.23 SK
Vanguard,
AAC A120 6.24 0.13 13.26 1.36 15.70 0.42 12.99 0.39 10.43 0.43 36.77 1.23 SK
Vanguard,
AGR044-312D 6.31 0.10 13.58 1.20 15.94 0.62 12.50 0.56 10.64 0.17 37.63 0.84
SK
Winner, SD AAC A110 5.90 0.11 13.16 0.92 17.24 0.84 12.42 0.20 8.90 0.44 38.72 1.06
Winner, SD AAC A120 6.08 0.05 14.10 0.87 17.32 0.70 11.59 0.31 9.60 0.39 38.32 0.22
Winner, SD AGR044-312D 6.36 0.10 16.33 1.06 19.13 0.45 9.81 0.16 9.19 0.27 37.87 0.82
Tioga, ND AAC A120 6.30 0.11 13.42 1.66 17.51 0.74 12.99 0.74 7.91 0.74 39.19 0.74
Tioga, ND AAC A110 6.23 0.05 10.49 1.76 15.89 0.74 13.51 0.74 8.80 0.74 39.77 0.74
Tioga, ND AGR044-3A22 6.30 0.05 11.66 1.46 17.00 0.74 12.74 0.74 9.49 0.74 38.08 0.74
Winner, SD AAC A120 6.29 0.25 14.68 0.91 19.36 0.74 11.17 0.74 8.32 0.74 36.76 0.74
Winner, SD AAC A110 6.27 0.02 13.39 3.05 18.44 0.74 11.77 0.74 8.66 0.74 37.92 0.74
Winner, SD AGR044-3A22 6.10 0.06 14.76 0.61 18.20 0.74 11.30 0.74 10.15 0.74 36.26 0.74
[0420] Example 4: AGR044-312D yield performance in Florida
[0421] Two small plot yield trials were carried out in Quincy, FL during the winter of 2014- 2015 comprising 20 entries and had four replications per entry in a randomized complete block design. The sites differ in that one field contained no traces of residual Cadre Herbicide (a commonly used herbicide in the southeastern United States, but whose residue might be of concern to potential carinata growers), while the other was on a piece that did have carry over of residual Cadre herbicide from previous crops. Seed yield estimates were calculated from the least square means of replicated plots estimated in kg per hectare and/or bushel per acre. Least square mean values and their standard errors were compared via REM L analysis (using student method of LS mean comparison) to determine whether differences were significant. Values that do not share a group letter are significantly different at P<0.05. All statistical analysis was performed using the JMP statistical analysis software (SAS). [0422] As can be seen in Table 8, yields of AG 044-312D were significantly higher than checks in both the Cadre residue and no residue sites. The entry AGR044-312D Improved on A120 yields by 123% and A110 by 126% in the no residue site and likewise improved on A120 by 109% and AllO by 112% in cadre residue containing site. There did not appear to be a large difference between the site with Cadre residue and the no cadre site.
Table 8: AGR044-312D yields relative to checks in Quincy FL yield trials (Winter 2014-2015)
[0423] Example 5: AGR044-312D Agronomic traits in Florida
[0424] Frost tolerance: In Florida, carinata is grown as a winter cover crop, seeded optimally in November. As such it is exposed to a period where frost is a persistant risk. Evidence has indicated that carinata is quite frost tolerant when exposed in early spring / late fall in its more northerly ranges and commercial varieties are assessed on their ability to recover after frost damage. Due to the fact that short periods of hard frost are an annual risk for carinata's use as a winter grown crop, assessing frost tolerance characteristics in new varieties is an important breeding consideration. In the 2014-15 Florida yield trials, two consecutive nights reaching -9°C provided good frost pressure to evaluate differences in yield trial entries. Frost tolerance was assessed on all plots at the two sites one, two, and three weeks following these hard frost events. This was done by determining the relative amount of damaged or dead plants in each plot, where 1 represents no damage and 10 represents all plants being killed. Table 9 illustrates post-frost ratings for AGR044-312D and the two check varieties, at each of the three time periods following the hard frost event. More freeze damage occurred at the No Residual site than the Cadre residual site. No major differences, positive or negative, were noted in these trials between 312D and the check varieties.
Table 9: Frost tolerance tolerance of AGR044-312D compared with checks in Quincy, FL, 2014-15
No Residual - More freeze damage Cadre residual - Less freeze damage
Name 1 wk 2 wks 3 wks Name 1 wk 2 wks 3 wks
AAC AllO 8 AGR044-312D 9 5 4
AAC A120 9 AAC AllO 9 5 4
AG 044-312D 9 AAC A120 9 6 5
Ratings: 1-10, indicating % damage. 1 = no damage; 10 = dead (ratings taken weekly after freeze event)
[0425] Maturity ratings: Days to maturity is an important factor in selection of new varieties. In Florida, as a winter cover crop, it is important that carinata matures and is harvested early enough to allow for timely seeding of the spring cash crops, such as peanuts, soybean, sesame, etc. Days to flowering is flowering is typically correlated with days to maturity, as earlier flowering and completion of flowering allows for earlier seed maturation. Accordingly, days to flowering and days to maturity were evaluated for AGR044-312D in relation to the check lines AllO and A120. At the no residue site, mean days to flowering occurred significantly earlier for the check lines than for AGR044-312D (by 2.5 days). Mean days to maturity for AllO occurred 1.8 days earlier than AGR044-312D while mean days to maturity for A120 occurred 3.3 days earlier than AGR044-312D, the latter difference being significant. It is not clear whether fertility would affect the maturity ratings. Thus, in the winter of 2014-15, 312D matured slightly later than the check varieties.
Table 10: Maturity ratings at the No Residue site in 2014-15 Quincy, FL yield trials
[0426] Example 6: AGR044-312D seed quality data from Quincy Florida (2014-2015)
[0427] Seed harvested from the 2014- 2015 Florida yield trial sites were used for seed quality analysis using the same methodology as described in earlier examples
[0428] Oil content: As can be seen in Table 11, the mean oil content of the AGR044-312D seed was slightly lower compared to those of the check-lines A110 and A120, however this difference was not statistically significant. Table 11: Seed quality versus check lines for oil content of312D in 2014-15 Quincy, FL yield testing - Least Square Mean of two sites
[0429] GSL content: Similar to what was observed in the North Dakota, South Dakota and
Saskatchewan trials, GSL content of AGR044-312D seed harvested from Florida trials (Table 12) showed a significant reduction relative to check lines A110 and A120 (in the order of a 40% reduction).
Table 12: Seed quality versus check lines for glucosinolate content of AGR044- 312D in 2014-15 Quincy, FL yield testing - Least Square Mean of two sites
[0430] Seed protein content: Table 13 compares the protein contents of AGR044-312D with those of check lines A110 and A120 in seed harvested from the 2014-2015 Florida field trials. At both sites in 2014-15 Quincy, FL yield testing, the AGR044-312D line had approximately 2% lower protein content on a whole seed basis than the check varieties.
Table 13: Seed protein levels of A110, A120 and AGR044-312D expressed as percentage of seed weight from 2014-15 Quincy, FL yield testing
[0431] Fatty Acid Profile: Table 14 shows the fatty acid profile of AGR044-312D and check lines A110 and A120 from grain harvested from 2014- 2015 Florida trials. Similar to that of the North Dakota, South Dakota and Saskatchewan trials, the VLCFA erucic acid (C22.1) proportion in AGR044-312D oil falls very close to those of the commercial check lines in these trials, and likewise levels of saturated fatty acid (SATS) of AGR044-312D oil are close to those of the check lines. The relative proportions of the other main fatty acid constituents of AGR044-312D oil do not deviate substantially from those of the check line counterparts. Thus, it is expected that the physical properties of AGR044-312D oil that are influenced by fatty acid composition should not differ greatly from those of the check lines.
Table 14: Fatty acid profiles of AGR044- 312D, A120 and AllO oil, as determined by NIR analysis, from 2014-15 Quincy, FL yield testing
[0432] Example 7: Preparation of DNA from Brassica carinata A110, A120, AGR044-312D and AGR044-3A22 leaf tissue
[0433] Approximately 50-100 mg leaf tissues was sampled from Brassica carinata plants and placed into a sterile 1.5 ml microfuge tube on ice using forceps sterilized by dipping in 70% ethanol or 2% solution of sodium hypochlorite (NaCIO) and wiping off between samples to avoid possible cross contamination. The tubes were then sealed with Parafilm and a fine-tipped forceps was used to make approximately 2-4 small holes in the Parafilm seal. The samples were then placed at -80°C overnight (minimally greater than 12 hours).
[0434] After the -80°C incubation, the samples were loaded directly from -80°C into freeze drying apparatas and lyophilized for a minimum of 42 hours. Following the lyphilization, the parafilm seals were removed from the tubes and replaced with the tube lids. At this point samples could be stored at -20°C for up to two weeks before processing for extraction of DNA.
[0435] To extract genomic DNA from the lyophilized samples two 3 mm glass beads (Sigma or any general suppliers) were placed into each tube. Tubes were then capped and loaded on a bead beater and processed twice for 30 s each time. Tubes were then centrifuged briefly to collect contents at the bottom and 500 μΙ of extraction buffer (2% CTAB, 100 mM Tris, pH 8, 20 mM EDTA, 1.4M NaCI ; before use beta-mercaptoethanol was added at ratio of 4 μΙ/ml of extraction buffer) was added to each sample tube and mixed by inversion, ensuring that all the lyophilized powder was solubilized, then placed at 65°C for 1 h. 500 μΙ of chloroform was then added and to each sample then content of the tubes was mixed by inversion for 5 minutes. Sample tubes were then centrifuged for 10 min at 13,000 RPM and then 400 μΙ of aqueous supernatant phase was transferred to new tubes containing 250 μΙ of isopropanol, mixed by inversion and incubated at room temperature for 10 minutes to overnight. DNA pellets were collected by centrifugation at 13,000 rpm for 15 min. Pellets were washed 2X by addition of 250 μΙ of 70% followed by centrifugation at 13000RPM for 1 min and removal of supernatant. After the completion of the second wash, the tubes were briefly centrifuged to collect any residual liquid , and the last drops of wash was removed with a micropipettor. The DNA pellets were dried at room temperature for 5 minutes, and then resuspended in 50 μΙ 0.1 x TE (for PCR) or 1 x TE (for other downstream work) containing 1 μΙ of RNase A (lOmg/ml). Genomic DNA (gDNA) was quantified by picogreen fluorescence using the Quanti-IT DS DNA assay kit (Invitrogen) according to the manufacturers instructions and sample concentrations were normalized to 20 ng/μΙ.
[0436] Example 8: GBS library generation and QC from DNA from Brassica carinata A110, A120, AGR044-312D and AGR044-3A22
[0437] GBS libraries were generated essentially as described (Poland et al. 2012). Briefly, 10 μΙ of DNA (20 ng/μΙ) was double digested with restriction enzymes Pst\ (Psfl-HF, NEB, Cat.# R3140)and Msp\ (NEB, Cat.# R0106) by incubating at 37°C for 2 hrs, then 65°C for 20 min. Digested DNA was ligated to adapters by adding 5 μΙ of Adapters (0.02 μΜ Adapter 1 = 0.1 pmol, 3 μΜ Adapter 2 = 15 pmol) and 15 μΙ ligation mix (2 μΙ NEB Buffer 4, 4 μΙ ATP (lOmM), 0.5 μΙ T4 DNA ligase (200 U)), and 8.5 μΙ H 2 0. The reaction was incubated at 22°C for 2 h, and then 65°C for 20 min. Five μΙ from each sample ligation was pooled into a single tube and cleaned on a Qiagen column (QIAquick PCR Purification Kit (Qiagen, Cat#: 28106)). Eight PCR reactions were made for each library: 10 μΙ DNA (digested library), 5 μΙ Taq 5 X Master Mix (NEB, Cat# M0285S), and 8 μΙ H 2 0. PCR was run following this program: 95°C, 30 s.; 16 cycles of 95°C, 30 s, 62°C, 30 s, and 68°C for 30 s; followed by extension at 72°C for 5 min. The 8 PCR reactions were pooled and cleaned using QIAquick PCR Purification Kit following the manufacturer's directions and
resuspended in 30 μΙ IX TE buffer (pH 8.0) with addition of 1 μΙ of RNase A (10 mg/μΙ). Prior to sequencing the GBS library was checked for quality on Agilent 2100 Bioanalyzer using the Agilent DNA 1000 Kit (Cat: 5067-1504) to ensure that a majority DNA fragments ranged from 150-250 bp in size.
[0438] Example 9: GS library sequence analysis
[0439] The libraries were multiplexed at 96-fold and sequenced as 100 bp paired-end reads on an lllumina Hiseq2000™ according to the manufacturer's instructions. The raw sequence data were then de-multiplexed into the 96 individual samples). The software package Trimmomatic
(http://www.usadellab.org/cms/?page=trimmomatic) was used to remove short sequences, adapter sequences, trim leading and trailing low quality regions and scan the read to cut bases when the average quality per base dropped below the threshold. Duplicate reads were then removed.
[0440] Cleaned sequence reads were aligned to the reference genome B. nigra (B) and B. oleracea (C) using Bowtie2 (Langmead and Salzberg S, 2012). The parameters were set as : --local --sensitive -- phred33 -minins 0 -maxins 1000 --no-mixed --no-discordant --dovetail -k 50 -score-min L,0,0.8. The best hit from any multi-mapped reads were then selected. SNPs were called using the genome analysis toolkit (GATK) using an UnifiedGenotyper (Mackenna et al. 2010). Of the raw SNPs, high quality SNPs were selected using the following criteria: minor allele frequency (MAF) >0.05, missing data in less than 30% of the lines, heterozygosity of < 0.1, and read depth of >4.
[0441] Example 10: Comparison of polymorphic SNPs (determined by GBS) found in A110, A120, AGR044-312D and AGR044-3A22 varieties
[0442] A GBS approach (genotype by sequencing) was used to identify a set of allelic markers that can be used to identify Brassica carinata varieties AGR044-312D and AGR044-3A22. The allelic markers are mainly single nucleotide polymorphisms (SNPs), but there are also some indel (insertion/deletion) markers. Varieties A110 and A120 were used as controls.
[0443] In Tables 16, 17 and 18 the positions of SNP (and Indel) mutations determined by GBS that are polymorphic among the A110, A120, AGR044-312D and AGR044-3A22 lines are tabulated and mapped to Brassica carinata chromosomes. Table 15 shows examples of contiguous chromosomal regions containing SNPS/indels that are common among AGR044-312D and AGR044-3A22, Table 16 shows examples of contiguous chromosomal regions containing multiple SNPS/INDELS unique for AGR044- 312D, and Table 17 shows examples of contiguous chromosomal regions containing multiple
SNPS/INDELS unique for AGR044-3A22. The data provides a demonstration at the molecular level of the distinctiveness of the AGR044-312D and AGR044-3A22 varieties relative to current commercial carinata varieties.
[0444] Tables 18 through 34 each represent one of the Brassica carinata chromosomes. There are 17 chromosomes in total: eight B chromosomes, and 9 C chromosomes. Table 15: Examples of regions on Brassica carinata chromosome 1, 3, 6 and 11 with shared markers for two members of the AGR044 family
/+ - ++
+/- ++ -
Table 16: Examples of regions on Brassica carinata chromosome 1, 3, 6 and 11 with markers unique for AGR044 -312D
/+ -- ++ --
-/+ ++
+/- -
-/* ++ Table 17: Examples of regions on Brassica carinata chromosome 1, 2, 5, 8, 9 and 12 with markers unique for AGR044 -3A22
G6
· · ·.
6 -6
CC
TT
TT
CC T
AA
CC
AA
/l TT TT
CC
TT
TT
TT
6G
AA
6G
:c
CC
AA
TT
TT
1 CC
TT
GG
M
-Λ
G6
c TT
[ 1 CC
c TT
AA
66
TT
AA
-
CC
66
GG
TT
AA
AA
CC
TT
TT
AA
AA
M
TT
TT
CC
CC
AA
AA
AA
66
CC
TT
CC
CC Table 18: SNP and indel markers on carinata chromosome Bl.
Bl_4913370 C/G 1 4913370 CC CC GG CC
Bl_4913468 G/A 1 4913468 GG GG AA GG
Bl_5079804 C/T 1 5079804 CC CC TT CC
Bl_5079805 T/G 1 5079805 TT TT GG TT
Bl_5205202 +/- 1 5205202 - ++ ++ ++
Bl_5263885 A/G 1 5263885 AA GG GG GG
Bl_5433567 A/T 1 5433567 TT TT AA TT
Bl_5433574 A/T 1 5433574 AA AA TT AA
Bl_5433575 C/G/T 1 5433575 GG GG TT GG
Bl_5433576 A/T 1 5433576 TT TT AA TT
Bl_5433601 C/T 1 5433601 TT TT CC TT
Bl_5433606 G/A 1 5433606 AA AA GG AA
Bl_6091439 T/C 1 6091439 TT TT CC CC
Bl_6113949 C/T 1 6113949 TT TT CC TT
Bl_6113967 A/T 1 6113967 AA AA TT AA
Bl_6113973 C/T 1 6113973 CC CC TT CC
Bl_6113982 T/C 1 6113982 CC CC TT CC
Bl_6113994 G/C 1 6113994 CC CC GG CC
Bl_6219642 G/C 1 6219642 GG CC CC CC
Bl_6226291 T/C 1 6226291 CT CC CC CC
Bl_6226329 A/C 1 6226329 AC CC CC CC
Bl_6266950 T/C 1 6266950 TT CC CC CC
Bl_7196923 A/T 1 7196923 AA AA TT AA
Bl_7375544 G/A 1 7375544 GG GG AA GG
Bl_7375562 G/A 1 7375562 GG GG AA GG
Bl_7375570 T/G 1 7375570 TT TT GG TT
Bl_7375574 T/C 1 7375574 TT TT CC TT
Bl_7375619 C/T 1 7375619 CC CC TT CC
Bl_7375694 T/C 1 7375694 TT TT CC TT
Bl_7385056 A/G 1 7385056 AG AA AA AA
Bl_7385080 G/T 1 7385080 GT GG GG GG
Bl_7385107 G/T 1 7385107 GG GG TT GG
Bl_8098708 T/C 1 8098708 TT CC CC CC
Bl_8098839 G/A 1 8098839 GG AA AA AA
Bl_8099096 T/C 1 8099096 TT CC CC CC
Bl_8497566 A/C 1 8497566 AC AA CC AA
Bl_8497593 C/T 1 8497593 CT CC TT CC
Bl_8617833 C/T 1 8617833 TT CC CC CC
Bl_8657230 C/T 1 8657230 CT CC CC CC Bl_8657231 C/A 1 8657231 AC CC CC CC
Bl_8657252 A/T 1 8657252 AT AA AA AA
Bl_8657264 +/- 1 8657264 -+ ++ ++ ++
Bl_8657287 T/A 1 8657287 AT TT TT TT
Bl_8796643 T/C 1 8796643 CC TT TT TT
Bl_8938410 A/G 1 8938410 GG AA GG GG
Bl_9040408 T/A 1 9040408 TT TT AA TT
Bl_9076501 T/G 1 9076501 TT TT GG TT
Bl_9903740 G/C 1 9903740 CC CC GG GG
Bl_9903773 C/T 1 9903773 TT TT CC CC
Bl_11358777 A/G 1 11358777 AA AA GG AA
Bl_11358794 T/G 1 11358794 TT TT GG TT
Bl_11358803 T/C 1 11358803 TT TT CC TT
Bl_11358810 C/T 1 11358810 CC CC TT CC
Bl_11358815 G/A 1 11358815 GG GG AA GG
Bl_11358848 G/A 1 11358848 GG GG AA GG
Bl_11358852 A/G 1 11358852 AA AA GG AA
Bl_11358853 C/T 1 11358853 CC CC TT CC
Bl_11358895 A/T 1 11358895 AA AA TT AA
Bl_11451599 G/C 1 11451599 GG GG CC GG
Bl_11451607 G/A 1 11451607 GG GG AA GG
Bl_12593164 C/T 1 12593164 CC TT TT TT
Bl_12689423 A/T 1 12689423 TT AA AA AA
Bl_13625960 A/G 1 13625960 AA GG GG GG
Bl_13633115 A/T 1 13633115 AA TT TT TT
Bl_13633146 A/G 1 13633146 AA GG GG GG
Bl_13633153 C/T 1 13633153 CC TT TT TT
Bl_13633167 G/T 1 13633167 GG TT TT TT
Bl_13633169 A/G 1 13633169 AA GG GG GG
Bl_13867050 A/G 1 13867050 AA AA GG AA
Bl_13867158 C/T 1 13867158 CC CC TT CC
Bl_13934008 T/A 1 13934008 TT TT AA TT
Bl_13934047 T/A 1 13934047 TT TT AA TT
Bl_13941235 G/T 1 13941235 GG GG TT GG
Bl_14344065 +/- 1 14344065 - - ++ ++
Bl_14621432 A/T 1 14621432 AA AA TT AA
Bl_15171944 T/C 1 15171944 TT CC TT CC
Bl_15171975 +/- 1 15171975 ++ - ++ -
Bl_15171977 +/- 1 15171977 ++ - ++ -
Ill Bl_15171978 +/- 1 15171978 ++ - ++ -
Bl_15519937 A/G 1 15519937 AA AA GG AA
Bl_15684572 C/T 1 15684572 CC CC TT CC
Bl_15684590 +/- 1 15684590 ++ ++ -- ++
Bl_15684592 +/- 1 15684592 ++ ++ -- ++
Bl_15684600 +/- 1 15684600 ++ ++ -- ++
Bl_15684746 C/G 1 15684746 CC CC GG CC
Bl_15697210 C/T 1 15697210 CC CC TT CC
Bl_15718053 A/T 1 15718053 AA AA TT AA
Bl_15827845 A/T 1 15827845 AA AA TT AA
Bl_15828085 -/+ 1 15828085 - - ++ -
Bl_16017282 T/C 1 16017282 TT CC TT CC
Bl_16152353 C/T 1 16152353 CC TT CC TT
Bl_16152360 G/A 1 16152360 GG AA GG AA
Bl_16152370 A/G 1 16152370 AA GG AA GG
Bl_16152374 C/A 1 16152374 CC AA CC AA
Bl_16152375 G/A 1 16152375 GG AA GG AA
Bl_16152413 G/C 1 16152413 GG CC GG CC
Bl_16152447 T/A 1 16152447 TT AA TT AA
Bl_16152448 T/G 1 16152448 TT GG TT GG
Bl_16285720 G/T 1 16285720 GG GG GG TT
Bl_16285732 T/G 1 16285732 TT TT TT GG
Bl_16285738 G/A 1 16285738 GG GG GG AA
Bl_18583848 G/T 1 18583848 GT GG GG GG
Bl_18583885 A/C 1 18583885 AC AA AA AA
Bl_22802512 C/T 1 22802512 CC CC TT TT
Bl_23003189 C/A 1 23003189 CC CC AA CC
Bl_33983139 +/- 1 33983139 - ++ ++ -
Bl_34061998 A/C 1 34061998 CC CC AA AA
Bl_34062026 A/T 1 34062026 TT TT AA AA
Bl_34062030 A/T 1 34062030 TT TT AA AA
Bl_34062032 T/A 1 34062032 AA AA TT TT
Bl_34316809 G/C 1 34316809 CC CC GG GG
Bl_34465544 T/G 1 34465544 GG TT TT TT
Bl_34465574 T/C 1 34465574 CC CC TT TT
Bl_36734256 G/C 1 36734256 CC CC GG GG
Bl_36734421 T/C 1 36734421 CC CC TT TT
Bl_36734436 C/T 1 36734436 TT TT CC CC
Bl_36734454 A/G 1 36734454 GG GG AA AA Bl_36734457 G/T 1 36734457 TT TT GG GG
Bl_36989449 A/G 1 36989449 AA AA AA GG
Bl_36989614 C/T 1 36989614 CC CC CC TT
Bl_37008771 C/T 1 37008771 CC TT CC CC
Bl_37235018 G/T 1 37235018 GG GG TT GG
Bl_37235020 G/T 1 37235020 GG GG TT GG
Bl_37235037 C/T 1 37235037 CC CC TT CC
Bl_37235248 T/G 1 37235248 TT TT GG TT
Bl_37235251 C/T 1 37235251 CC CC TT CC
Bl_37235289 A/T 1 37235289 AA AA TT AA
Bl_37235761 C/G 1 37235761 GG GG CC GG
Bl_37388943 A/G 1 37388943 GG GG AA AA
Bl_37388973 T/A 1 37388973 AA AA TT TT
Bl_37877639 T/C 1 37877639 TT TT CC TT
Bl_37913522 A/G 1 37913522 AA AA AA GG
Bl_37916594 +/- 1 37916594 ++ ++ ++ -
Bl_37916596 A/G 1 37916596 AA AA AA GG
Bl_37916597 A/C 1 37916597 AA AA AA CC
Bl_37916614 G/T 1 37916614 GG GG GG TT
Bl_37916616 C/T 1 37916616 CC CC CC TT
Bl_37916631 A/C 1 37916631 AA AA AA CC
Bl_37916634 C/T 1 37916634 CC CC CC TT
Bl_37916653 G/A 1 37916653 GG GG GG AA
Bl_37916656 A/C 1 37916656 AA AA AA CC
Bl_37916659 G/A 1 37916659 GG GG GG AA
Bl_37922785 +/- 1 37922785 - ++ ++
Bl_38841681 T/G 1 38841681 TT GG TT TT
Bl_38968377 A/T 1 38968377 AA AA AA TT
Bl_38968533 A/C 1 38968533 CC AA AA CC
Bl_38968534 T/A 1 38968534 AA AA TT AA
Bl_39055759 G/A 1 39055759 GG GG AA GG
Bl_39178523 C/G 1 39178523 GG GG CC GG
Bl_39178745 C/T 1 39178745 TT TT CC TT
Bl_39179533 T/A 1 39179533 AA AA TT AA
Bl_39179568 C/T 1 39179568 TT TT CC TT
Bl_39179623 T/A 1 39179623 AA AA TT AA
Bl_39179626 T/C 1 39179626 CC CC TT CC
Bl_39184869 A/T 1 39184869 TT TT AA TT
Bl_39184879 T/G 1 39184879 GG GG TT GG Bl_39184901 T/C 1 39184901 CC CC TT CC
Bl_39184910 T/C 1 39184910 CC CC TT CC
Bl_39184921 C/T 1 39184921 TT TT CC TT
Bl_39415871 +/- 1 39415871 ++ ++ -- ++
Bl_39415872 +/- 1 39415872 ++ ++ -- ++
Bl_39415876 -/+ 1 39415876 - - ++ -
Bl_39415930 G/C 1 39415930 GG GG CC GG
Bl_39574365 C/T 1 39574365 CC CC CC TT
Bl_39574372 A/G 1 39574372 AA AA AA GG
Bl_39574582 C/G 1 39574582 CC CC CC GG
Bl_39574602 T/C 1 39574602 TT TT TT CC
Bl_39682794 T/C 1 39682794 TT TT CC TT
Bl_39683005 C/G 1 39683005 CC CC GG CC
Bl_39704139 A/T 1 39704139 TT TT TT AA
Bl_39704142 A/G 1 39704142 GG GG GG AA
Bl_39704156 A/G 1 39704156 GG GG GG AA
Bl_40778920 +/- 1 40778920 ++ ++ ++ ++
Bl_41330761 T/C 1 41330761 CC CC CC TT
Bl_41330901 A/G 1 41330901 GG AA GG GG
Bl_41330908 T/A 1 41330908 AA AA AA TT
Bl_41989952 A/G 1 41989952 AA AA GG AA
Table 19: SNP and indel markers on carinata chromosome B2.
B2_2046354 A/G 2 2046354 GG GG AA AA
B2_2046467 T/C 2 2046467 CC CC TT TT
B2_2095931 A/G 2 2095931 GG GG AA AA
B2_2490513 A/G 2 2490513 GG AA AA AA
B2_2685089 A/G 2 2685089 GG GG AA AA
B2_2685159 A/G 2 2685159 GG GG AA AA
B2_2685171 7+/A 2 2685171 ++ ++
B2_2685171 A/G 2 2685171 GG GG AA AA
B2_2685177 +/- 2 2685177 - - ++ ++
B2_2817238 C/T 2 2817238 CC TT TT TT
B2_2817245 C/G 2 2817245 GG CC CC CC
B2_2817306 A/T 2 2817306 AA TT TT TT
B2_2817345 +/- 2 2817345 ++ -
B2_2817349 +/- 2 2817349 - ++ ++ ++
B2_2869441 C/G 2 2869441 GG CC GG GG
B2_5613898 T/C 2 5613898 TT TT CC TT
B2_5705108 C/T 2 5705108 CC CC TT TT
B2_5705132 C/T 2 5705132 CC CC TT TT
B2_5705250 G/A 2 5705250 GG GG AA AA
B2_5705300 G/C 2 5705300 GG GG CC CC
B2_5705318 A/G 2 5705318 AA AA GG AA
B2_8647348 A/G 2 8647348 GG GG AA AA
B2_17033118 A/G 2 17033118 AA AA GG GG
B2_17253174 A/G 2 17253174 GG AA GG AA
B2_21926129 C/T 2 21926129 CC CC TT CC
B2_21926133 A/G 2 21926133 GG AA GG AA
B2_25376864 A/G 2 25376864 GG GG AA AA
B2_25376941 A/T 2 25376941 TT TT AA AA
B2_25376948 T/A 2 25376948 AA AA TT TT
B2_25376953 2 25376953 ++ ++
B2_25433916 T/C 2 25433916 TT TT CC CC
B2_25607162 T/C 2 25607162 CC CC TT TT
B2_25607173 T/G 2 25607173 GG GG TT TT
B2_25607176 T/A 2 25607176 AA AA TT TT
B2_25607251 T/A 2 25607251 AA AA TT TT
B2_25614867 A/C 2 25614867 AA AA CC CC
B2_25614886 C/T 2 25614886 CC CC TT TT
B2_25844297 +/- 2 25844297 - ++ ++ ++
B2_26402164 A/T 2 26402164 TT AA AA AA
B2_26402171 C/A 2 26402171 CC CC AA CC
B2_27092960 +/- 2 27092960 - ++ ++ ++ B2_27718601 C/T 2 27718601 TT CC CC CC
B2_27718638 C/T 2 27718638 TT CC CC CC
B2_28002506 G/T 2 28002506 TT GG GG GG
B2_30578322 A/G 2 30578322 GG AA GG AA
B2_30578333 A/T 2 30578333 TT AA TT AA
B2_30578361 +/- 2 30578361 - ++ ++
B2_30589836 A/G 2 30589836 AA GG AA AA
B2_30589837 T/A 2 30589837 TT AA TT TT
B2_30589907 A/T 2 30589907 AA TT AA AA
B2_30716778 +/- 2 30716778 - ++
B2_30894547 C/T 2 30894547 cc CC TT TT
B2_31283579 C/G 2 31283579 GG CC CC CC
B2_31788221 C/T 2 31788221 CC CC TT CC
B2_31926404 A/C 2 31926404 CC CC AA AA
B2_31926458 T/C 2 31926458 CC CC TT TT
B2_31989632 C/T 2 31989632 TT TT CC CC
B2_32076325 G/A 2 32076325 GG GG AA GG
B2_32076356 A/G 2 32076356 AA AA GG AA
B2_32076508 G/C 2 32076508 GG GG CC GG
B2_32076509 A/T 2 32076509 AA AA TT AA
B2_32076561 A/G 2 32076561 AA AA GG AA
B2_32076577 T/C 2 32076577 TT TT CC TT
B2_32183288 G/A 2 32183288 GG AA GG AA
B2_32183294 A/G 2 32183294 AA GG AA GG
B2_32183433 G/T 2 32183433 GG TT GG TT
B2_33093633 T/C 2 33093633 TT TT TT CC
B2_33093639 C/T 2 33093639 CC CC CC TT
B2_33093717 C/T 2 33093717 CC CC CC TT
B2_33093724 C/T 2 33093724 CC CC CC TT
B2_33093732 A/G 2 33093732 AA AA AA GG
B2_33093768 G/A 2 33093768 GG GG GG AA
B2_33093782 A/G 2 33093782 AA AA AA GG
B2_33889056 C/T 2 33889056 CC TT CC CC
B2_33889059 G/A 2 33889059 GG AA GG GG
B2_35073237 +/- 2 35073237 - ++ ++
B2_35134541 G/A 2 35134541 GG AA AA AA
B2_35146912 G/A 2 35146912 GG AA AA AA
B2_35146915 G/T 2 35146915 GG TT TT TT
B2_35147035 G/C 2 35147035 GG GG CC GG
B2_35147036 T/C 2 35147036 TT TT CC TT
B2_35147037 G/T 2 35147037 GG GG TT GG B2_35147038 G/A 2 35147038 GG GG AA GG
B2_35147040 G/C 2 35147040 GG GG CC GG
B2_35147075 T/A 2 35147075 TT TT AA TT
B2_35147077 C/A 2 35147077 CC CC AA CC
B2_35314554 A/T 2 35314554 TT AA TT TT
B2_35314563 T/G 2 35314563 GG TT GG GG
B2_35314717 C/T 2 35314717 CC CC TT TT
B2_35314729 A/G 2 35314729 AA AA GG GG
B2_35906658 A/C 2 35906658 AA AA CC AA
B2_35906661 A/G 2 35906661 AA AA GG AA
B2_35906832 G/C 2 35906832 GG GG CC GG
B2_36131519 T/C 2 36131519 TT TT CC CC
B2_36297563 C/T 2 36297563 CC TT TT CC
B2_36341182 A/G 2 36341182 GG GG AA GG
B2_36845065 T/C 2 36845065 CC TT TT TT
B2_36845091 G/A 2 36845091 GG AA GG GG
B2_36845212 C/G 2 36845212 CC CC GG CC
B2_36845223 A/T 2 36845223 AA TT AA AA
B2_36845252 T/G 2 36845252 GG TT TT TT
B2_36848565 A/C 2 36848565 AA CC AA AA
B2_36849003 C/A 2 36849003 CC CC AA CC
B2_36849004 A/G 2 36849004 AA AA GG AA
B2_36849006 T/C 2 36849006 TT TT CC TT
B2_36849009 A/G 2 36849009 AA AA GG AA
B2_37088988 G/A 2 37088988 GG AA GG AA
B2_37089079 C/A 2 37089079 CC AA CC CC
B2_37089144 C/A 2 37089144 CC AA CC CC
B2_37089168 C/A 2 37089168 CC AA CC CC
B2_37089203 T/A 2 37089203 TT AA TT TT
B2_37196101 +/- 2 37196101 ++ -
B2_37196102 C/T 2 37196102 CC TT TT TT
B2_37196129 C/T 2 37196129 CC TT TT TT
B2_37596308 T/C 2 37596308 CC TT TT TT
B2_37596432 G/C 2 37596432 CC GG GG GG
B2_37605168 T/G 2 37605168 GG TT TT TT
B2_37605365 T/G 2 37605365 TT TT GG GG
B2_38148274 C/T 2 38148274 CC TT CC CC
B2_38148299 T/C 2 38148299 TT CC TT TT
B2_38195542 T/G 2 38195542 GG TT TT TT
B2_38195545 C/A 2 38195545 AA CC CC CC
B2_38195584 G/C 2 38195584 CC GG GG GG B2_38195588 T/C 2 38195588 CC TT TT TT
B2_38994537 T/A 2 38994537 AA TT TT TT
B2_38994583 T/G 2 38994583 GG TT TT TT
B2_39085736 C/T 2 39085736 CC TT CC CC
B2_39182584 A/C 2 39182584 CC AA AA AA
B2_39182692 C/G 2 39182692 GG CC CC CC
B2_39182694 G/T 2 39182694 TT GG GG GG
B2_39182696 G/T 2 39182696 TT GG GG GG
B2_39182697 G/A 2 39182697 AA GG GG GG
B2_39182700 T/C 2 39182700 CC TT TT TT
B2_39182701 C/A 2 39182701 AA CC CC CC
B2_39182721 C/T 2 39182721 TT CC CC CC
B2_39182729 T/C 2 39182729 CC TT TT TT
B2_39186529 A/C 2 39186529 CC AA AA AA
B2_39186576 T/C 2 39186576 CC TT TT TT
B2_39186628 G/A 2 39186628 AA GG GG GG
B2_39832793 A/G 2 39832793 GG GG GG AA
B2_39832817 G/A 2 39832817 AA AA GG GG
B2_39838840 G/C 2 39838840 CC CC GG GG
B2_39838847 +/- 2 39838847 - - ++ ++
B2_39843974 G/A 2 39843974 AA AA GG GG
B2_39843986 C/T 2 39843986 CC CC TT TT
B2_39924831 C/T 2 39924831 CC CC TT TT
B2_39924852 G/A 2 39924852 GG GG AA AA
B2_39934892 T/C 2 39934892 TT TT CC CC
B2_40636742 +/- 2 40636742 - ++ ++
B2_41185174 +/- 2 41185174 ++ ++ ++
B2_41185187 T/C 2 41185187 TT TT CC TT
B2_41185350 G/A 2 41185350 GG GG AA GG
B2_41435165 T/A 2 41435165 TT TT AA AA
B2_41435202 A/C 2 41435202 AA AA CC CC
B2_41435204 G/C 2 41435204 GG GG CC CC
B2_41435206 T/A 2 41435206 TT TT AA AA
B2_41438344 C/T 2 41438344 CC CC TT TT
B2_41438375 C/T 2 41438375 CC CC TT TT
B2_41438383 G/A 2 41438383 GG GG AA AA
B2_41438420 C/T 2 41438420 CC CC TT TT
B2_41438443 G/C 2 41438443 GG GG CC CC
B2_41438444 G/C 2 41438444 GG GG CC CC
B2_41438445 A/T 2 41438445 AA AA TT TT
B2_41438507 A/T 2 41438507 AA AA TT TT B2_41704942 T/C 2 41704942 TT TT CC CC
B2_41704954 C/T 2 41704954 CC CC TT TT
B2_41704957 C/G 2 41704957 CC CC GG GG
B2_41704981 T/G 2 41704981 TT TT GG GG
B2_41704996 A/C 2 41704996 AA AA CC CC
B2_41704999 A/C 2 41704999 AA AA CC CC
B2_41705005 G/C 2 41705005 GG GG CC CC
B2_41900188 A/G 2 41900188 AA AA GG AA
B2_41900299 C/T 2 41900299 TT TT CC CC
B2_41900304 C/T 2 41900304 TT TT CC CC
B2_41900305 A/G 2 41900305 GG GG AA AA
B2_41900319 A/G 2 41900319 GG GG AA AA
B2_41900327 -/+ 2 41900327 ++ ++ -- --
B2_41900330 -/+ 2 41900330 ++ ++ -- --
B2_41900338 A/G 2 41900338 GG GG AA AA
B2_41900439 A/G 2 41900439 GG GG AA AA
B2_41900441 T/C 2 41900441 CC CC TT TT
B2_41900480 C/T 2 41900480 TT TT CC CC
B2_41900496 G/A 2 41900496 AA AA GG GG
B2_41930864 -/+ 2 41930864 ++ ++ -- --
B2_42627565 T/C 2 42627565 TT TT CC TT
B2_42897201 C/T 2 42897201 TT CC CC CC
B2_42897211 +/- 2 42897211 - ++ ++ ++
B2_42897214 A/C 2 42897214 CC AA AA AA
B2_42897226 G/A 2 42897226 AA GG GG GG
B2_42897273 C/T 2 42897273 TT CC CC CC
B2_42897274 A/C 2 42897274 CC AA AA AA
B2_42897275 T/C 2 42897275 CC TT TT TT
B2_42897281 T/C 2 42897281 CC TT TT TT
B2_42897282 G/A 2 42897282 AA GG GG GG
B2_42939943 A/T 2 42939943 TT AA AA AA
B2_43112370 C/G 2 43112370 CC GG CC GG
B2_43112519 C/T 2 43112519 CC TT CC TT
B2_43164140 T/C 2 43164140 TT TT TT CC
B2_43168208 T/G 2 43168208 TT TT TT GG
B2_43255370 T/C 2 43255370 TT CC TT TT
B2_43298937 G/A 2 43298937 AA GG GG GG
B2_43298961 C/T 2 43298961 TT CC CC CC
B2_43298963 A/G 2 43298963 GG AA AA AA
B2_43299009 T/C 2 43299009 CC TT TT TT
B2_43299053 G/T 2 43299053 TT GG GG GG B2_43536933 -/+ 2 43536933 ++ ++ -- ++
B2_43537125 C/A 2 43537125 CC CC AA CC
B2_43756448 -/+ 2 43756448 - - ++ --
B2_43756451 A/G 2 43756451 AA GG AA GG
B2_43756484 T/C 2 43756484 TT TT CC TT
B2_43756508 T/C 2 43756508 TT TT CC CC
B2_43756580 C/T 2 43756580 CC CC TT CC
B2_43925481 A/C/G 2 43925481 AA CC AA AA
B2_43925514 G/A 2 43925514 GG AA GG GG
B2_43925565 C/A 2 43925565 CC AA CC CC
B2_44395214 G/A 2 44395214 GG AA AA AA
B2_44445324 T/A 2 44445324 TT TT TT AA
B2_44951868 A/T 2 44951868 TT TT AA AA
B2_44951873 T/A 2 44951873 AA AA TT TT
B2_44951876 T/A 2 44951876 AA AA TT TT
B2_44952207 A/T 2 44952207 TT TT AA AA
B2_44952232 C/T 2 44952232 TT TT CC CC
B2_44952318 -/+ 2 44952318 - ++ -- --
B2_44952352 G/C 2 44952352 GG CC GG GG
B2_45019981 T/C 2 45019981 TT CC TT TT
B2_45019990 T/C 2 45019990 TT CC TT TT
B2_45139358 C/T 2 45139358 CC CC CC TT
B2_45139370 A/G 2 45139370 GG AA AA GG
B2_45139421 T/G 2 45139421 TT TT TT GG
B2_45584234 T/A 2 45584234 TT AA TT TT
B2_45797405 G/T 2 45797405 GT GG GG GG
B2_45797469 A/G 2 45797469 AG GG AA GG
B2_45797535 A/G 2 45797535 AG GG AA GG
B2_45797548 C/T 2 45797548 CT TT CC TT
B2_45797561 A/T 2 45797561 AT TT AA TT
B2_46124179 G/A 2 46124179 AA GG GG GG
B2_46152800 C/T 2 46152800 CC TT CC CC
B2_46152828 A/G 2 46152828 AA GG AA AA
B2_46482001 C/T 2 46482001 TT CC CC CC
B2_46482055 G/A 2 46482055 AA GG GG GG
B2_46664691 T/A 2 46664691 TT TT TT AA
B2_46664714 G/C 2 46664714 GG GG GG CC
B2_46664891 C/T 2 46664891 CC CC CC TT
B2_46735650 -/+ 2 46735650 ++ - -- ++
B2_46741860 G/C 2 46741860 CC GG GG CC
B2_46741887 T/G 2 46741887 GG TT TT GG B2_46985529 A/T 2 46985529 TT AA AA AA
B2_46985577 T/C 2 46985577 CC TT TT TT
B2_46985611 A/C 2 46985611 CC AA AA AA
B2_46999719 T/G 2 46999719 GG TT TT TT
B2_47083570 A/G 2 47083570 GG AA GG AA
B2_47083593 T/C 2 47083593 CC TT CC TT
B2_47153449 C/A 2 47153449 CC CC CC AA
B2_47153491 G/A 2 47153491 GG GG GG AA
B2_47509345 C/T 2 47509345 CC CC TT CC
B2_48740610 T/A 2 48740610 AA AA TT TT
B2_48740639 A/C 2 48740639 CC CC AA AA
B2_49872143 T/C 2 49872143 TT TT CC TT
B2_49872220 T/A 2 49872220 TT TT AA TT
B2_49872271 G/C 2 49872271 GG GG CC GG
B2_51345834 C/T 2 51345834 CC CC CC TT
B2_51345868 C/T 2 51345868 CC CC CC TT
B2_51345870 A/G 2 51345870 AA AA AA GG
B2_51365120 G/A 2 51365120 GG GG GG AA
B2_51365137 +/- 2 51365137 ++ ++ ++
B2_51444825 T/C 2 51444825 TT TT TT CC
B2_51534879 T/C 2 51534879 TT CC TT TT
Table 20: SNP and indel markers on carinata chromosome B3.
B3_2494869 G/A 3 2494869 GG GG AA AA
B3_2494890 G/A 3 2494890 GG GG AA AA
B3_2804073 T/C 3 2804073 TT CC TT TT
B3_2804085 C/T 3 2804085 CC TT CC CC
B3_2804115 T/C 3 2804115 TT TT CC CC
B3_2804118 A/G 3 2804118 AA AA GG GG
B3_2804130 T/C 3 2804130 TT TT CC CC
B3_2804145 G/T 3 2804145 GG TT GG GG
B3_2804148 G/A 3 2804148 AA GG GG GG
B3_2804163 G/A 3 2804163 AA GG GG GG
B3_2864742 A/G 3 2864742 AA AA GG GG
B3_2864811 C/A 3 2864811 CC CC AA AA
B3_3070053 G/A 3 3070053 GG GG AA AA
B3_3188238 +/- 3 3188238 ++ - ++ ++
B3_3188251 T/C 3 3188251 TT CC TT TT
B3_3188252 +/- 3 3188252 ++ - ++ ++
B3_3188286 C/T 3 3188286 CC TT CC CC
B3_3253668 +/- 3 3253668 ++ -
B3_3304470 3 3304470 ++ -
B3_3304557 T/C 3 3304557 CC TT TT TT
B3_3381623 G/C 3 3381623 GG GG CC CC
B3_3381644 A/G 3 3381644 AA AA GG GG
B3_3381650 T/G 3 3381650 TT TT GG GG
B3_3381686 A/G 3 3381686 AA AA GG GG
B3_3381693 A/G 3 3381693 AA AA GG GG
B3_3381706 T/G 3 3381706 TT TT GG GG
B3_3421459 A/G 3 3421459 GG AA AA AA
B3_3421463 C/T 3 3421463 TT CC CC CC
B3_3421583 G/T 3 3421583 TT GG GG GG
B3_3421605 C/T 3 3421605 TT CC CC CC
B3_3421638 C/A 3 3421638 AA CC CC CC
B3_3547083 G/A 3 3547083 AA GG GG GG
B3_3547098 C/A 3 3547098 AA CC CC CC
B3_3547135 G/A 3 3547135 AA GG GG GG
B3_3547206 A/G 3 3547206 GG AA AA AA
B3_3551663 G/A 3 3551663 GG AA GG GG
B3_3551750 T/A 3 3551750 TT AA TT TT
B3_3551915 G/T 3 3551915 GG TT GG GG
B3_3551919 G/A 3 3551919 GG AA GG GG
B3_3551975 T/C 3 3551975 TT CC TT TT
B3_3564741 C/G 3 3564741 CC GG CC CC B3_3564747 G/A 3 3564747 GG AA GG GG
B3_3570723 A/C 3 3570723 CC CC AA AA
B3_3731442 G/A 3 3731442 GG GG AA AA
B3_3731489 C/T 3 3731489 CC CC TT TT
B3_3731493 T/C 3 3731493 TT TT CC CC
B3_3731499 C/T 3 3731499 CC CC TT TT
B3_3731508 A/C 3 3731508 AA AA CC CC
B3_4200470 T/A 3 4200470 TT TT AA AA
B3_4200528 C/T 3 4200528 CC CC TT TT
B3_4200558 T/C 3 4200558 TT TT CC CC
B3_4273336 +/- 3 4273336 -- ++ - --
B3_4542443 C/A 3 4542443 AA CC CC CC
B3_4981971 C/A 3 4981971 AA CC AA AA
B3_5010464 A/T 3 5010464 AA AA TT TT
B3_5010515 A/G 3 5010515 AA AA GG GG
B3_5010525 T/G 3 5010525 TT TT GG GG
B3_5010526 G/T 3 5010526 GG GG TT TT
B3_5225938 A/G 3 5225938 GG AA AA AA
B3_5225962 C/T 3 5225962 TT CC CC CC
B3_5225968 A/T 3 5225968 TT AA AA AA
B3_5225992 -/+ 3 5225992 ++ - - --
B3_5343538 -/+ 3 5343538 ++ - - --
B3_5343541 C/T 3 5343541 TT CC CC CC
B3_5343559 T/C 3 5343559 CC TT TT TT
B3_5343562 T/C 3 5343562 CC TT TT TT
B3_5343579 G/A 3 5343579 AA GG GG GG
B3_5343585 G/A 3 5343585 AA GG GG GG
B3_5343633 T/G 3 5343633 GG TT TT TT
B3_5343643 C/T 3 5343643 TT CC CC CC
B3_6790711 G/A 3 6790711 GG GG AA AG
B3_6790728 -/+ 3 6790728 -- - ++ ++
B3_6790733 C/T 3 6790733 CC CC TT CT
B3_6995373 -/+ 3 6995373 -- - ++ ++
B3_6995388 G/A 3 6995388 GG GG AA AA
B3_8129537 +/- 3 8129537 -- -+ ++ ++
B3_9105106 A/G 3 9105106 GG AA AA AA
B3_10562264 T/G 3 10562264 GG TT TT TT
B3_10562288 -/+ 3 10562288 ++ - - --
B3_10562291 T/G 3 10562291 GG TT TT TT
B3_10587725 A/G 3 10587725 GG GG AA GG
B3_10587730 +/- 3 10587730 -- - ++ -- B3_10587732 A/G 3 10587732 AA AA GG AA
B3_10587924 C/T 3 10587924 TT TT CC TT
B3_10794776 G/C 3 10794776 GG GG CC GG
B3_10794966 A/G 3 10794966 AA AA GG AA
B3_10794984 G/A 3 10794984 GG GG AA AA
B3_11821666 T/A 3 11821666 AA TT TT TT
B3_11881808 +/- 3 11881808 ++ ++ ++
B3_11881837 C/A 3 11881837 AA CC CC CC
B3_11881925 C/G 3 11881925 GG CC CC CC
B3_11881931 G/A 3 11881931 AA GG GG GG
B3_11952635 T/A 3 11952635 TT AA TT AA
B3_11952747 T/A 3 11952747 TT AA TT AA
B3_11962347 C/T 3 11962347 CC TT CC TT
B3_11962350 T/G 3 11962350 TT GG TT GG
B3_11962353 C/A 3 11962353 CC AA CC AA
B3_12302228 C/T 3 12302228 TT CC CC CC
B3_12624798 A/T 3 12624798 AA AT AA TT
B3_12717198 T/C 3 12717198 CC TT TT TT
B3_12717260 T/C 3 12717260 CC TT TT TT
B3_12717275 C/T 3 12717275 TT CC CC CC
B3_12717293 A/G 3 12717293 GG AA AA AA
B3_13065542 C/T 3 13065542 TT CC CC CC
B3_13088838 G/A 3 13088838 AG GG GG GG
B3_13088922 G/A 3 13088922 AG GG GG GG
B3_13088951 G/T 3 13088951 GT GG GG GG
B3_13101374 +/- 3 13101374 ++ - ++ ++
B3_13101450 A/G 3 13101450 AA GG AA AA
B3_13123469 +/- 3 13123469 ++ ++ ++
B3_13123489 C/T 3 13123489 TT CC CC CC
B3_13123505 T/C 3 13123505 CC TT TT TT
B3_13123516 C/A 3 13123516 AA CC CC CC
B3_13738924 A/C 3 13738924 AA AA CC AA
B3_13739100 C/T 3 13739100 CC CC TT CC
B3_13739124 C/T 3 13739124 CC CC TT CC
B3_14481530 G/T 3 14481530 GG GT GG GG
B3_14481545 A/T 3 14481545 AA AT AA AA
B3_14491306 A/G 3 14491306 AA AG AA AA
B3_15347180 A/G 3 15347180 AA AA GG GG
B3_15429126 T/C 3 15429126 TT TT CC TT
B3_16244765 G/A 3 16244765 GG AG AA GG
B3_16501177 C/T 3 16501177 CC CC TT TT B3_16501191 G/A 3 16501191 GG GG AA AA
B3_16501192 A/T 3 16501192 AA AA TT TT
B3_16501243 A/G 3 16501243 AA AA GG GG
B3_16501246 A/G 3 16501246 AA AA GG GG
B3_16677881 T/G 3 16677881 GG GT TT GG
B3_16677883 G/T 3 16677883 TT GT GG TT
B3_17062482 T/C 3 17062482 TT TT TT CT
B3_18547409 A/G 3 18547409 AA GG AA GG
B3_18718974 A/C 3 18718974 AA CC AA AA
B3_18836852 T/G 3 18836852 GG TT GG GG
B3_18836873 C/T 3 18836873 CC CC TT TT
B3_18836883 A/T 3 18836883 AA AA TT TT
B3_18836995 -/+ 3 18836995 -- - ++ ++
B3_18836996 -/+ 3 18836996 -- - ++ ++
B3_18837013 T/C 3 18837013 CC TT CC CC
B3_18837018 A/C 3 18837018 AA AA CC CC
B3_19124739 G/C 3 19124739 GG CC GG GG
B3_19124742 A/G 3 19124742 AA GG AA AA
B3_19124743 +/- 3 19124743 ++ - ++ ++
B3_19124847 A/G 3 19124847 AA GG AA AA
B3_19124870 C/T 3 19124870 CC TT CC CC
B3_19124897 G/A 3 19124897 GG AA GG GG
B3_19130886 T/A 3 19130886 AA TT AA AA
B3_19130910 T/A 3 19130910 AA TT AA AA
B3_19130961 T/C 3 19130961 CC TT CC CC
B3_19978214 A/G 3 19978214 AA GG GG GG
B3_19978353 G/A 3 19978353 GG GG AG GG
B3_20127326 G/T 3 20127326 GG TT GG GG
B3_22492905 G/C 3 22492905 CC GG CC CC
B3_22541095 G/C 3 22541095 CC GG CC CC
B3_22541096 G/C 3 22541096 CC GG CC CC
B3_22541138 G/A 3 22541138 AA GG AA AA
B3_22541202 C/T 3 22541202 TT CC TT TT
B3_22541223 G/A 3 22541223 AA GG AA AA
B3_23610585 T/C 3 23610585 TT CC TT TT
B3_23610676 T/C 3 23610676 TT CC TT TT
B3_23610680 +/- 3 23610680 -- ++ - --
B3_23835994 -/+ 3 23835994 -- - ++ ++
B3_23836017 A/G 3 23836017 AA AA GG GG
B3_23836019 G/A 3 23836019 GG GG AA AA
B3_23836169 G/A 3 23836169 GG GG AA AA B3_24012257 +/- 3 24012257 -- ++ ++ ++
B3_26904173 T/C 3 26904173 TT CC TT TT
B3_26904209 +/- 3 26904209 ++ - ++ ++
B3_26904210 G/C 3 26904210 GG CC GG GG
B3_26904210 +/-/G/C 3 26904210 ++ - ++ ++
B3_26904211 +/- 3 26904211 ++ - ++ ++
B3_26904235 +/- 3 26904235 ++ - ++ ++
B3_26904236 +/- 3 26904236 ++ - ++ ++
B3_27244575 T/C 3 27244575 CC TT CC CC
B3_27380725 T/C 3 27380725 cc TT CC CC
B3_27380744 C/A 3 27380744 AA CC AA AA
B3_27381123 +/- 3 27381123 -- ++ - --
B3_27428548 T/C 3 27428548 CC TT CC CC
B3_27556078 +/- 3 27556078 -- - - ++
B3_27556087 +/- 3 27556087 -- ++ - --
B3_27786577 C/T 3 27786577 cc CC TT TT
B3_27795547 A/G 3 27795547 GG AA GG GG
B3_27795548 G/T 3 27795548 TT GG TT TT
B3_28452841 -/+ 3 28452841 -- ++ - ++
B3_28632155 +/- 3 28632155 -- - ++ ++
B3_28641082 A/G 3 28641082 AA AA GG AA
B3_28641288 A/G 3 28641288 AA AA GG AA
B3_28641315 A/G 3 28641315 AA AA GG AA
B3_29578734 T/G 3 29578734 GG TT TT GG
B3_30636951 A/G 3 30636951 AA AA GG AA
B3_30636977 C/T 3 30636977 CC CC TT CC
B3_30636986 A/G 3 30636986 AA AA GG AA
B3_30637010 T/G 3 30637010 TT TT GG TT
B3_30873236 T/C 3 30873236 TT TT CC TT
B3_30903011 G/A 3 30903011 GG GG AA GG
B3_30903084 G/C 3 30903084 GG GG CC GG
B3_31949735 A/G 3 31949735 AA AA AA GG
B3_32536648 C/T 3 32536648 TT CC CC TT
B3_32753593 G/A 3 32753593 GG AA GG GG
B3_32831672 +/- 3 32831672 -- - ++ ++
B3_33499765 G/A 3 33499765 GG AA GG GG
B3_36439867 G/A 3 36439867 GG AA GG GG
B3_37825487 T/C 3 37825487 TT TT CC TT
B3_40289368 +/- 3 40289368 -- ++ ++ --
B3_42213222 +/- 3 42213222 -- ++ ++ ++
B3_44283940 G/A 3 44283940 AA GG GG GG B3_45408330 A/G 3 45408330 GG AA AA GG
B3_45408546 G/T 3 45408546 TT GG GG TT
B3_45408581 C/T 3 45408581 TT CC CC TT
B3_45462102 T/C 3 45462102 CC TT TT CC
B3_45462655 C/T 3 45462655 CC CC CC TT
B3_45915537 +/- 3 45915537 ++ ++
Table 21: SNP and indel markers on carinata chromosome B4.
B4_6273931 A/G 4 6273931 AA AA GG AA
B4_6273942 C/T 4 6273942 CC CC TT CC
B4_6273945 A/C 4 6273945 AA AA CC AA
B4_6273954 -/+ 4 6273954 - - ++ --
B4_6273958 T/C 4 6273958 TT TT CC TT
B4_6273964 A/G 4 6273964 AA AA GG AA
B4_6274002 C/T 4 6274002 CC CC TT CC
B4_6464630 A/G 4 6464630 AA AA GG AA
B4_6464661 -/+ 4 6464661 - - ++ --
B4_6464706 A/C 4 6464706 AA AA CC AA
B4_6464774 C/G 4 6464774 CC CC GG CC
B4_6464783 T/A 4 6464783 TT TT AA TT
B4_6464785 +/- 4 6464785 ++ ++ - ++
B4_6464789 +/- 4 6464789 ++ ++ - ++
B4_7096712 +/- 4 7096712 - ++ ++ ++
B4_7678055 C/A 4 7678055 AA AA CC AA
B4_7678082 G/A 4 7678082 AA AA GG AA
B4_7678130 C/A 4 7678130 AA AA CC AA
B4_7878224 G/T 4 7878224 TT TT GG TT
B4_7878260 C/G 4 7878260 GG GG CC GG
B4_7878263 G/A 4 7878263 AA AA GG AA
B4_7878453 G/A 4 7878453 AA AA GG AA
B4_7878497 A/G 4 7878497 GG GG AA GG
B4_8034824 T/G 4 8034824 TT TT GG TT
B4_8034841 C/T 4 8034841 CC CC TT CC
B4_8034865 G/T 4 8034865 GG GG TT GG
B4_8034876 T/C 4 8034876 TT TT CC TT
B4_8040373 A/G 4 8040373 AA AA GG AA
B4_8040374 C/T 4 8040374 CC CC TT CC
B4_8040386 T/C 4 8040386 TT TT CC TT
B4_8040417 G/A 4 8040417 GG GG AA GG
B4_8645443 T/C 4 8645443 TT TT CC TT
B4_8645469 C/T 4 8645469 CC CC TT CC
B4_8645500 T/C 4 8645500 TT TT CC TT
B4_8645505 C/A 4 8645505 CC CC AA CC
B4_8645522 T/C 4 8645522 TT TT CC TT
B4_8645588 A/G 4 8645588 AA AA GG AA
B4_8779280 G/A 4 8779280 GG GG AA GG
B4_8779305 C/G 4 8779305 CC CC GG CC
B4_8779316 G/A 4 8779316 GG GG AA GG
B4_8779341 C/A 4 8779341 CC CC AA CC B4_8779359 C/G 4 8779359 cc CC GG CC
B4_8779383 T/G 4 8779383 TT TT GG TT
B4_9274073 -/+ 4 9274073 ++ ++ - ++
B4_9274116 G/C 4 9274116 cc CC GG CC
B4_9303364 G/T 4 9303364 TT TT GG TT
B4_9314848 A/G 4 9314848 GG GG AA GG
B4_9505302 G/A 4 9505302 AA AA GG AA
B4_9505309 T/C 4 9505309 CC CC TT CC
B4_9505319 G/A 4 9505319 AA AA GG AA
B4_10778612 G/A 4 10778612 AA AA GG GG
B4_11728606 C/A 4 11728606 AA AA CC AA
B4_11728615 -/+ 4 11728615 ++ ++ - ++
B4_12040572 A/G 4 12040572 GG GG AA GG
B4_12040676 -/+ 4 12040676 ++ ++ - ++
B4_12955219 A/G 4 12955219 GG GG GG AA
B4_12955381 C/T 4 12955381 TT TT TT CC
B4_12955382 A/G 4 12955382 GG GG GG AA
B4_12955385 C/G 4 12955385 GG GG GG CC
B4_12955390 T/A 4 12955390 AA AA AA TT
B4_12955395 G/C 4 12955395 CC CC CC GG
B4_12955401 G/A 4 12955401 AA AA AA GG
B4_12955406 T/G 4 12955406 GG GG GG TT
B4_12955414 T/C 4 12955414 CC CC CC TT
B4_13032550 G/A 4 13032550 GG GG GG AA
B4_13032579 A/G 4 13032579 AA AA AA GG
B4_13032615 A/T 4 13032615 AA AA AA TT
B4_13032617 -/+ 4 13032617 - - - ++
B4_13032624 -/+ 4 13032624 - - - ++
B4_13032630 +/- 4 13032630 ++ ++ ++ --
B4_13367056 +/- 4 13367056 - - ++ ++
B4 14997272 T/C 4 14997272 TT TT TT CC
B4 14997312 G/C 4 14997312 GG GG GG cc
B4_15035777 C/T 4 15035777 CT CC CC cc
B4_15035778 T/A 4 15035778 AT TT TT TT
B4_15035815 T/A 4 15035815 AT TT TT TT
B4_15035822 G/T 4 15035822 GT GG GG GG
B4_15129592 -/+ 4 15129592 -+ ++ ++ --
B4_15129625 T/C 4 15129625 CT CC CC TT
B4_15143892 G/A 4 15143892 GG GG AA GG
B4_15143933 T/G 4 15143933 TT TT GG TT
B4_15144145 C/T 4 15144145 CC CC TT CC B4_15176017 G/A 4 15176017 GG GG AA GG
B4_15176035 C/T 4 15176035 CC CC TT CC
B4_15176043 C/T 4 15176043 CC CC TT CC
B4_15213015 C/A 4 15213015 CC CC AA CC
B4_15213695 C/T 4 15213695 CC CC TT CC
B4_15599186 A/T 4 15599186 TT TT AA TT
B4_15599198 A/G 4 15599198 GG GG AA GG
B4_15599252 A/T 4 15599252 AA AA TT AA
B4_15599259 C/A 4 15599259 CC CC AA CC
B4_15599263 C/T 4 15599263 CC CC TT CC
B4_15763700 -/+ 4 15763700 - - ++ ++
B4_15763731 +/- 4 15763731 ++ ++ - --
B4_15763774 A/C 4 15763774 AA AA CC CC
B4_15807883 T/C 4 15807883 TT TT CC CC
B4_15807901 T/C 4 15807901 TT TT CC CC
B4_15807925 A/C 4 15807925 AA AA CC CC
B4_15807988 T/C 4 15807988 TT TT CC CC
B4_16812189 -/+ 4 16812189 - - ++ --
B4_17686905 G/C 4 17686905 GG GG CC GG
B4_17686913 G/A 4 17686913 GG GG AA GG
B4_17686999 C/T 4 17686999 CC CC TT CC
B4_17687000 C/G 4 17687000 CC CC GG CC
B4_17687009 A/G 4 17687009 AA AA GG AA
B4_17687060 A/T 4 17687060 AA AA TT AA
B4_18161537 A/G 4 18161537 AA GG AA AA
B4_18161684 A/C 4 18161684 AA CC AA AA
B4_19231744 +/- 4 19231744 - ++ ++ --
B4_21663621 A/G 4 21663621 AA GG AA AA
B4_26338330 C/T 4 26338330 TT CC CC TT
B4_26440675 +/- 4 26440675 - ++ ++ ++
B4_29825295 G/T 4 29825295 TT GG GG TT
B4_30910722 G/A 4 30910722 AA GG GG AA
B4_30910723 G/A 4 30910723 AA GG GG AA
B4_32977910 A/G 4 32977910 GG AA AA GG
B4_34112222 G/A 4 34112222 AA AA GG AA
B4_34194700 G/A 4 34194700 GG GG AA AA
B4_34469464 G/A/T 4 34469464 AA GG GG AA
B4_36869424 +/- 4 36869424 - ++ ++ --
B4_40078197 G/A/T 4 40078197 GG GG GG AA Table 22: SNP and indel markers on carinata chromosome B5.
B5_1763371 C/T 5 1763371 CT TT CC CC
B5_1763789 C/A 5 1763789 AC AA CC CC
B5_1782666 T/C 5 1782666 CC TT TT CC
B5_1782711 G/C 5 1782711 CC GG GG CC
B5_1782713 G/A 5 1782713 AA GG GG AA
B5_1782791 A/G 5 1782791 GG AA AA GG
B5_1790874 T/G 5 1790874 TT TT GG TT
B5_1790901 G/A 5 1790901 GG GG AA GG
B5_1790955 A/G 5 1790955 AA AA GG AA
B5_1790970 C/A 5 1790970 CC CC AA CC
B5_1790991 A/G 5 1790991 AA AA GG AA
B5_1790994 A/G 5 1790994 AA AA GG AA
B5_1791010 G/T 5 1791010 GG GG TT GG
B5_1791021 C/T 5 1791021 CC CC TT CC
B5_1841778 C/A 5 1841778 AA CC CC CC
B5_1841894 G/T 5 1841894 TT GG GG GG
B5_1841960 A/T 5 1841960 TT AA AA AA
B5_1861239 G/A 5 1861239 AG GG GG GG
B5_1861284 T/A/C 5 1861284 AT TT TT TT
B5_1861290 A/C 5 1861290 AC AA AA AA
B5_1985401 C/T 5 1985401 CC CC CC TT
B5_2048942 G/C 5 2048942 GG GG GG CC
B5_2143463 C/A 5 2143463 CC CC CC AA
B5_2484395 G/T 5 2484395 GG GG GG TT
B5_2484401 A/T 5 2484401 AA AA AA TT
B5_2484428 T/C 5 2484428 TT TT TT CC
B5_2484431 C/T 5 2484431 CC CC CC TT
B5_2484560 C/G 5 2484560 CC CC CC GG
B5_2484562 G/A 5 2484562 GG GG GG AA
B5_2484563 -/+ 5 2484563 - - - ++
B5_2484571 A/G 5 2484571 AA AA AA GG
B5_2484602 C/T 5 2484602 CC CC CC TT
B5_2591800 C/A 5 2591800 AA CC AA CC
B5_2683680 +/- 5 2683680 - - - ++
B5_2835657 T/C 5 2835657 TT TT TT CC
B5_3444344 C/T 5 3444344 CC CC TT CC
B5_3534020 A/T 5 3534020 AA AA TT TT
B5_3534173 A/C 5 3534173 AA AA CC CC
B5_3534194 G/A 5 3534194 GG GG AA AA
B5_3859008 C/A 5 3859008 CC AA CC CC
B5_4795509 T/C 5 4795509 TT TT CC CC B5_4795512 T/A 5 4795512 TT TT AA AA
B5_4795584 A/T 5 4795584 AA AA TT TT
B5_5074559 C/T 5 5074559 CC CC TT TT
B5_5074601 G/A 5 5074601 GG GG AA AA
B5_5538479 C/G 5 5538479 CC CC GG GG
B5_5538482 G/A 5 5538482 GG GG AA AA
B5_5547873 T/C 5 5547873 TT TT CC CC
B5_5548068 -/+ 5 5548068 - - ++ ++
B5_5576514 T/C 5 5576514 CC CC TT TT
B5_6663994 +/- 5 6663994 - ++ - ++
B5_6996053 A/C 5 6996053 AA AA CC AA
B5_6996090 C/T 5 6996090 CC CC TT CC
B5_7026124 A/G 5 7026124 AA AA GG GG
B5_7026190 G/T 5 7026190 GG GG TT TT
B5_7217793 T/A 5 7217793 TT AA TT AA
B5_7401822 A/G 5 7401822 GG AA GG AA
B5_7401825 C/T 5 7401825 TT CC TT CC
B5_7478206 A/T 5 7478206 TT AA TT AA
B5_7478223 +/- 5 7478223 - ++ - ++
B5_7478238 T/C 5 7478238 CC TT CC TT
B5_7478248 -/+ 5 7478248 ++ - ++ --
B5_7478254 C/G 5 7478254 GG CC GG CC
B5_7478259 A/C 5 7478259 CC AA CC AA
B5_7478264 T/A 5 7478264 AA TT AA TT
B5_7661348 T/C 5 7661348 CC TT CC TT
B5_7661349 G/A 5 7661349 AA GG AA GG
B5_7661490 A/G 5 7661490 AA AA GG AA
B5_7661517 G/T 5 7661517 GG GG TT GG
B5_7765102 C/A 5 7765102 AA CC AA CC
B5_7765140 C/T 5 7765140 TT CC TT CC
B5_7787292 T/A 5 7787292 AA AA TT TT
B5_7787293 A/G 5 7787293 AA AA GG AA
B5_7787321 T/A 5 7787321 TT TT TT AA
B5_8754651 G/A 5 8754651 AA GG GG GG
B5_8754654 G/A 5 8754654 AA GG GG GG
B5_8754804 C/G 5 8754804 GG CC CC CC
B5_8754870 G/A 5 8754870 GG GG AA GG
B5_8754888 A/G 5 8754888 AA AA GG AA
B5_8754895 C/G 5 8754895 CC CC GG CC
B5_8755098 A/T 5 8755098 TT AA TT AA
B5_8755131 G/A 5 8755131 GG GG AA GG B5_10191354 G/A 5 10191354 GG GG AA GG
B5_10191410 -/+ 5 10191410 - - ++ --
B5_10218994 G/A 5 10218994 GG GG AA GG
B5_10260059 T/C 5 10260059 TT TT CC TT
B5_10260113 T/C 5 10260113 TT TT CC TT
B5_10260125 G/A 5 10260125 GG GG AA GG
B5_10260131 C/G 5 10260131 CC CC GG CC
B5_10260293 G/C 5 10260293 GG GG CC GG
B5_10260296 T/G 5 10260296 TT TT GG TT
B5_10260299 T/G 5 10260299 TT TT GG TT
B5_10481826 C/G 5 10481826 CC GG GG CC
B5_10481968 C/A 5 10481968 CC AA AA CC
B5_10946750 A/T 5 10946750 AA TT AA TT
B5_10946817 C/T 5 10946817 CC TT CC TT
B5_10946847 C/T 5 10946847 CC TT CC TT
B5_10951191 T/C 5 10951191 TT CC TT CC
B5_10951231 T/G 5 10951231 TT GG TT GG
B5_10951296 T/G 5 10951296 TT GG TT GG
B5_11327481 C/T 5 11327481 TT CC CC CC
B5_11327582 T/G 5 11327582 GG TT TT TT
B5_11327583 C/A 5 11327583 AA CC CC CC
B5_12116907 A/G 5 12116907 GG GG AA GG
B5_13212345 G/A 5 13212345 GG GG GG AA
B5_13212388 +/- 5 13212388 - ++ ++ ++
B5_13648660 C/T 5 13648660 CC CC TT TT
B5_13842901 A/G 5 13842901 GG AA AA AA
B5_13842927 G/A 5 13842927 AA GG GG GG
B5_14836285 G/A 5 14836285 GG AA GG AA
B5_14836291 G/C 5 14836291 GG CC GG CC
B5_15324726 C/T/+/- 5 15324726 ++ CC CC CC
B5_17689974 +/- 5 17689974 - ++ ++ --
B5_20203884 G/T/A 5 20203884 GG GG TT GG
B5_24861520 +/- 5 24861520 - ++ ++ ++
B5_26077557 -/A/T/+ 5 26077557 AA AA ++ AA
B5_35549427 T/G 5 35549427 TT TT GG GG
B5_35760531 +/- 5 35760531 ++ ++ ++ --
B5_35760537 +/- 5 35760537 ++ ++ ++ --
B5_35760555 A/G 5 35760555 AA AA AA GG
B5_35760566 A/G 5 35760566 AA AA AA GG
B5_35760569 -/+ 5 35760569 - - - ++
B5_35865093 A/G 5 35865093 AA AA AA GG B5_35865114 G/C 5 35865114 GG GG GG CC
B5_35865155 C/T 5 35865155 CC CC CC TT
B5_35963051 G/C 5 35963051 GG GG GG CC
B5_35963075 C/T 5 35963075 CC CC CC TT
B5_35963092 +/- 5 35963092 ++ ++ ++ --
B5_35963096 A/+/C/- 5 35963096 AA ++ AA CC
B5_35963096 +/- 5 35963096 ++ ++ ++ --
B5_36095630 A/T 5 36095630 AA AA AA TT
B5_36095703 G/A 5 36095703 GG GG GG AA
B5_36582190 G/A 5 36582190 GG GG GG AA
B5_36582249 T/C 5 36582249 TT TT TT CC
B5_36799928 G/T 5 36799928 GG GG GG TT
B5_36799929 T/A 5 36799929 TT TT TT AA
B5_37036213 T/G 5 37036213 TT TT TT GG
B5_37036216 T/C 5 37036216 TT TT TT CC
B5_37036229 C/G 5 37036229 CC CC CC GG
B5_37315099 G/A 5 37315099 GG GG GG AA
B5_38188415 A/G 5 38188415 GG GG AA AA
B5_38188441 A/G 5 38188441 AA AA AA GG
B5_39332014 G/T 5 39332014 GG GG GG TT
B5_39350908 A/C 5 39350908 AA AA AA CC
B5_39350919 +/- 5 39350919 ++ ++ ++ --
B5_39350922 +/A/G/- 5 39350922 AA AA ++ --
B5_39350922 A/G 5 39350922 AA AA AA GG
B5_39350924 A/T 5 39350924 AA AA AA TT
B5_39351027 G/A 5 39351027 GG GG GG AA
B5_39401319 T/A 5 39401319 TT TT TT AA
B5_39401412 C/A 5 39401412 CC CC CC AA
B5_39401415 A/C 5 39401415 AA AA AA CC
B5_39401428 T/G 5 39401428 TT TT TT GG
B5_39401465 G/A 5 39401465 GG GG GG AA
B5_39402016 G/C 5 39402016 GG GG GG CC
B5_39811777 A/G 5 39811777 AA AA AA GG
B5_39811821 C/A 5 39811821 CC CC CC AA
B5_39811870 C/A 5 39811870 CC CC CC AA
B5_39847936 C/T 5 39847936 CC CC CC TT
B5_39847946 C/T 5 39847946 CC CC CC TT
B5_39848000 T/A 5 39848000 TT TT TT AA
B5_40452889 T/A 5 40452889 TT TT TT AA
B5_40452907 C/T 5 40452907 CC CC CC TT
B5_40453200 C/A 5 40453200 CC CC CC AA B5_40454085 G/A 5 40454085 GG GG GG AA
B5_40454118 A/T 5 40454118 AA AA AA TT
B5_40533370 +/- 5 40533370 - ++ ++ ++
B5_40767060 +/- 5 40767060 - ++ ++ ++
B5_40812570 C/A 5 40812570 CC CC AA CC
B5_42583833 T/C 5 42583833 cc CC TT CC
B5_42583855 A/G 5 42583855 GG GG AA GG
B5_42583857 C/T 5 42583857 TT TT CC TT
B5_42583865 G/T 5 42583865 TT TT GG TT
B5_42583893 A/T 5 42583893 TT TT AA TT
B5_42583914 A/G 5 42583914 GG GG AA GG
B5_42583920 C/T 5 42583920 TT TT CC TT
B5_42678186 T/A 5 42678186 AA AA TT AA
B5_42726375 C/T 5 42726375 TT TT CC TT
B5_43181323 G/A 5 43181323 GG GG AA GG
B5_43181351 G/A 5 43181351 AA AA GG AA
B5_43306325 A/C 5 43306325 CC CC AA CC
B5_43306340 C/A 5 43306340 AA AA CC AA
B5_43306366 G/A 5 43306366 AA AA GG AA
B5_43306395 T/C 5 43306395 CC CC TT CC
B5_43306415 T/A 5 43306415 AA AA TT AA
B5_43324578 C/A 5 43324578 AA AA CC AA
B5_43324620 T/C 5 43324620 TT TT CC TT
B5_43364130 C/T 5 43364130 CC CC TT CC
B5_46597606 G/T 5 46597606 GG GG TT TT
B5_48123651 A/G 5 48123651 AA AA GG AA
B5_50249647 G/T 5 50249647 GG GG TT GG
B5_50249666 T/C 5 50249666 TT TT CC TT
B5_50249847 G/A 5 50249847 GG GG AA GG
B5_50249855 A/G 5 50249855 AA AA GG AA
B5_50508090 G/C 5 50508090 CC CC GG CC
B5_50508112 C/A 5 50508112 AA AA CC AA
B5_50508345 -/+ 5 50508345 ++ ++ - ++
B5_50508397 A/C 5 50508397 CC CC AA CC
B5_50659557 T/G 5 50659557 GG GG TT GG
B5_50659572 T/C 5 50659572 CC CC TT CC
B5_50659575 C/T 5 50659575 TT TT CC TT
B5_50659713 C/G 5 50659713 GG GG CC GG
B5_51028431 A/C 5 51028431 AA CC CC CC Table 23: SNP and indel markers on carinata chromosome B6.
B6_4067767 C/T 6 4067767 TT TT CC TT
B6_4067773 C/T 6 4067773 TT TT CC TT
B6_4067788 G/A 6 4067788 AA AA GG AA
B6_4097407 G/A 6 4097407 AA AA GG AA
B6_4097455 T/C 6 4097455 CC CC TT CC
B6_4751128 G/A 6 4751128 AA AA GG AA
B6_4751324 G/A 6 4751324 AA AA GG AA
B6_4792541 A/G 6 4792541 AA AA GG AA
B6_4792583 G/A 6 4792583 GG AA AA GG
B6_4809241 C/A 6 4809241 CC CC AA CC
B6_4809272 A/G 6 4809272 AA AA GG AA
B6_4809303 A/T 6 4809303 AA AA TT AA
B6_4809318 A/G 6 4809318 AA AA GG AA
B6_4809416 C/G 6 4809416 CC CC GG CC
B6_4809417 A/G 6 4809417 AA AA GG AA
B6_4809445 G/A 6 4809445 GG GG AA GG
B6_4960047 C/T 6 4960047 CC CC CT CC
B6_5085115 A/G 6 5085115 AA AG AA AA
B6_5117858 G/T 6 5117858 TT TT GG TT
B6_5605561 +/- 6 5605561 - - ++ --
B6_5620862 A/G 6 5620862 AA AA GG AA
B6_5620901 T/C 6 5620901 TT TT CC TT
B6_5767212 A/C 6 5767212 AA AA CC AA
B6_5773353 +/- 6 5773353 ++ ++ - ++
B6_5773373 G/C 6 5773373 GG GG CC GG
B6_7156469 C/A 6 7156469 AA AA CC AA
B6_7156476 G/A 6 7156476 AA AA GG AA
B6_7156486 A/T 6 7156486 TT TT AA TT
B6_7156487 T/A 6 7156487 AA AA TT AA
B6_7156496 A/C 6 7156496 CC CC AA CC
B6_7156509 G/T 6 7156509 TT GG GG TT
B6_7156513 A/G 6 7156513 GG GG AA GG
B6_7156657 C/T 6 7156657 TT TT CC TT
B6_7318554 C/T 6 7318554 TT TT CC TT
B6_7318575 G/T 6 7318575 TT TT GG TT
B6_7318608 T/G 6 7318608 GG GG TT GG
B6_7475254 C/A 6 7475254 CC CC AA CC
B6_7482593 C/T 6 7482593 CC CC TT CC
B6_7482608 T/C 6 7482608 TT TT CC TT
B6_7482625 -/+ 6 7482625 - - ++ --
B6_7482630 C/A 6 7482630 CC CC AA CC B6_7482643 A/T 6 7482643 AA AA TT AA
B6_7482645 +/- 6 7482645 ++ ++ - ++
B6_7482648 +/- 6 7482648 ++ ++ - ++
B6_7482691 C/G 6 7482691 CC CC GG CC
B6_7482718 G/C 6 7482718 GG GG CC GG
B6_7482742 C/T 6 7482742 CC CC TT CC
B6_7603098 A/C 6 7603098 CC CC AA CC
B6_7644233 +/- 6 7644233 - ++ ++ ++
B6_7899968 A/C 6 7899968 AA AA CC AA
B6_7900001 +/- 6 7900001 ++ ++ - ++
B6_7977748 C/T 6 7977748 CC CC CC TT
B6_8600850 T/C 6 8600850 TT TT CC TT
B6_8600884 G/A 6 8600884 GG GG AA GG
B6_8651843 G/A 6 8651843 AA GG GG AA
B6_8651858 A/G 6 8651858 GG AA AA GG
B6_8886941 G/A 6 8886941 GG GG AA GG
B6_8886974 G/A 6 8886974 GG GG AA GG
B6_8887100 C/T 6 8887100 CC CC TT CC
B6_8993300 A/G 6 8993300 AA AA GG AA
B6_8993339 C/T 6 8993339 CC CC TT CC
B6_8993357 G/T 6 8993357 GG GG TT GG
B6_8993361 A/T 6 8993361 AA AA TT AA
B6_8993435 A/G 6 8993435 AA AA GG AA
B6_8993450 C/T 6 8993450 CC CC TT CC
B6_8994068 T/C 6 8994068 TT TT CC TT
B6_8994080 G/A 6 8994080 GG GG AA GG
B6_8994092 A/G 6 8994092 AA AA GG AA
B6_8994205 G/A 6 8994205 GG GG AA GG
B6_9166746 C/G 6 9166746 CC GC CC GG
B6_9166871 G/T 6 9166871 GG TT GG TT
B6_10064088 +/- 6 10064088 - ++ ++ ++
B6_13007972 +/- 6 13007972 - ++ ++ ++
B6_16264705 A/C 6 16264705 AA AA CC AA
B6_16267737 A/G 6 16267737 AA AA GG AG
B6_16417693 A/G 6 16417693 AA AA AA GG
B6_16478090 C/A 6 16478090 CC CC AA CC
B6_16714431 T/C 6 16714431 CC TT CC TT
B6_16714518 A/G 6 16714518 GG AA GG AA
B6_16714520 A/G 6 16714520 AA AA GG AA
B6_16956791 T/A 6 16956791 AA TT TT TT
B6_16997831 T/G 6 16997831 GG TT GG TT B6_16998944 A/C 6 16998944 AA AA CC AA
B6_16998984 +/- 6 16998984 ++ ++ - ++
B6_17004465 T/A 6 17004465 TT TT AA TT
B6_17004571 A/G 6 17004571 GG AA GG AA
B6_17167352 C/T 6 17167352 TT CC CC CC
B6_17167353 C/T 6 17167353 TT CC CC CC
B6_17168449 A/C 6 17168449 CC AA CC AA
B6_17168472 A/G 6 17168472 AA AA GG AA
B6_17168496 G/A 6 17168496 GG GG AA GG
B6_17168498 C/T 6 17168498 CC CC TT CC
B6_17168573 G/T 6 17168573 GG GG TT GG
B6_17184264 T/C 6 17184264 CC TT CC TT
B6_17258664 C/G 6 17258664 GG CC GG CC
B6_17272576 A/C 6 17272576 CC AA CC AA
B6_17445757 A/G 6 17445757 GG AA AA AA
B6_17445777 C/T 6 17445777 TT CC CC CC
B6_17445861 C/T 6 17445861 TT CC CC CC
B6_17484144 C/G 6 17484144 GG CC CC CC
B6_17545623 A/C 6 17545623 CC AA CC AA
B6_17617057 T/G 6 17617057 TT TT GG TT
B6_17617179 C/G 6 17617179 GG CC CC CC
B6_17629400 A/G 6 17629400 AA AA GG AA
B6_17931814 T/C 6 17931814 CC TT TT TT
B6_18195799 G/T 6 18195799 GG GG TT TT
B6_18737656 T/A 6 18737656 TT TT AA AA
B6_18910126 A/G 6 18910126 AA AA GG GG
B6_18961665 C/A 6 18961665 CC CC AA AA
B6_18961721 A/G 6 18961721 AA AA GG GG
B6_18961731 G/A 6 18961731 GG GG AA AA
B6_19031097 C/A 6 19031097 CC CC AA AA
B6_19090688 -/+ 6 19090688 - - ++ ++
B6_19107834 A/G 6 19107834 AA AA GG GG
B6_19107886 G/C 6 19107886 GG GG CC CC
B6_19107923 A/G 6 19107923 AA AA GG GG
B6_19143187 A/C 6 19143187 AA AA CC CC
B6_19387905 G/T 6 19387905 GG GG TT TT
B6_19689358 A/G 6 19689358 AA AA GG GG
B6_19933516 T/C 6 19933516 TT TT CC CC
B6_19933631 G/A 6 19933631 GG AG AA AA
B6_20015515 A/G 6 20015515 AA AA GG GG
B6_20015538 G/A 6 20015538 GG GG AA AA B6_20015615 A/C 6 20015615 AA AA CC CC
B6_20015622 C/T 6 20015622 CC CC TT TT
B6_20015695 +/- 6 20015695 ++ ++ - --
B6_20015752 C/T 6 20015752 CC CC TT TT
B6_20730653 T/G 6 20730653 TT TT TT GG
B6_20730671 C/A 6 20730671 CC CC CC AA
B6_20730683 T/C 6 20730683 TT TT TT CC
B6_20899097 C/G 6 20899097 CC CC GG CC
B6_20899115 +/- 6 20899115 ++ ++ - ++
B6_20899140 G/T 6 20899140 GG GG TT GG
B6_20899160 A/T 6 20899160 AA AA TT AA
B6_20966175 G/A 6 20966175 GG GG AA GG
B6_21009175 T/G 6 21009175 TT TT GG TT
B6_22690043 A/T 6 22690043 TT AA AA AA
B6_22690103 G/T 6 22690103 TT GG GG GG
B6_22911242 +/- 6 22911242 - - ++ ++
B6_22911272 C/T 6 22911272 TT TT CC CC
B6_22922426 T/A 6 22922426 AA AA TT TT
B6_22922427 C/A 6 22922427 AA AA CC CC
B6_25585131 C/T 6 25585131 TT CC CC CC
B6_25937289 C/A 6 25937289 AA CC CC CC
B6_25937312 A/G 6 25937312 GG AA AA AA
B6_26036960 G/A 6 26036960 AA GG GG GG
B6_26037102 G/A 6 26037102 AA GG GG GG
B6_26277314 C/A 6 26277314 AA CC CC CC
B6_27079710 G/C 6 27079710 CC CC GG GG
B6_27079755 C/G 6 27079755 GG CC CC CC
B6_27079896 T/A 6 27079896 AA AA TT TT
B6_27079911 T/A 6 27079911 AA AA TT TT
B6_28803769 A/G 6 28803769 GG GG AA AA
B6_29127428 -/+ 6 29127428 - - ++ ++
B6_29127454 C/A 6 29127454 CC CC AA AA
B6_29127456 +/- 6 29127456 ++ ++ - --
B6_29127552 C/T 6 29127552 CC CC TT TT
B6_29127604 A/G 6 29127604 AA AA GG GG
B6_29326118 T/C 6 29326118 TT TT CC TT
B6_29326130 A/C 6 29326130 AA AA CC AA
B6_29326193 G/A 6 29326193 GG GG AA GG
B6_29417432 C/T 6 29417432 CC CC TT CC
B6_29417442 C/T 6 29417442 CC CC TT CC
B6_29417530 T/G 6 29417530 TT TT GG TT B6_29417542 G/C 6 29417542 GG GG CC GG
B6_29417561 A/C 6 29417561 AA AA CC AA
B6_29417577 A/T 6 29417577 AA AA TT AA
B6_29714533 C/G 6 29714533 CC CC CC GG
B6_31055897 +/- 6 31055897 ++ ++ ++ --
B6_32132062 T/G 6 32132062 TT TT GG TT
B6_32373631 C/G 6 32373631 GC CC CC CC
B6_32373736 C/T 6 32373736 CC CC TT TT
B6_32373770 C/T 6 32373770 CC CC TT TT
B6_32373771 G/A 6 32373771 GG GG AA AA
B6_32397918 A/G 6 32397918 AA AA AA GG
B6_32397928 +/- 6 32397928 ++ ++ ++ --
B6_32435494 C/T 6 32435494 CC CC CC TT
B6_32435517 +/- 6 32435517 -+ ++ ++ --
B6_32435524 +/- 6 32435524 ++ ++ ++ --
B6_33224416 C/T 6 33224416 CC CC TT CC
B6_34318047 +/- 6 34318047 - - ++ ++
B6_34318092 C/T 6 34318092 TT TT CC CC
B6_34318145 C/T 6 34318145 TT TT CC CC
B6_34318200 A/G 6 34318200 GG GG AA AA
B6_35373956 T/G 6 35373956 TT TT GG TT
B6_35374048 C/A 6 35374048 CC CC AA AA
B6_35707458 T/A 6 35707458 AA TT TT TT
B6_35792938 T/A 6 35792938 AA TT AA AA
B6_35792980 G/T 6 35792980 TT GG TT TT
B6_35792994 T/C 6 35792994 CC TT CC CC
B6_36003826 T/C 6 36003826 CC CC TT TT
B6_36003893 T/G 6 36003893 GG GG TT TT
B6_36003896 G/T 6 36003896 TT TT GG GG
B6_36412972 A/G 6 36412972 AA AA GG GG
B6_36413017 +/- 6 36413017 ++ ++ - --
B6_36413018 +/- 6 36413018 ++ ++ - --
B6_36413018 A/C/+/- 6 36413018 AA AA CC CC
B6_36413019 +/- 6 36413019 ++ ++ - --
B6_36413019 A/G/+/- 6 36413019 AA AA GG GG Table 24: SNP and indel markers on carinata chromosome B7.
B7_2001294 G/T 7 2001294 TT TT GG GG
B7_2078547 A/G 7 2078547 AA AA AA GG
B7_2580651 C/T 7 2580651 TT TT CC CC
B7_2585406 C/A 7 2585406 AA AA CC CC
B7_2987169 T/A 7 2987169 AA AA TT AA
B7_3444681 A/G 7 3444681 GG GG AA AA
B7_3634860 T/A 7 3634860 AA AA AA TT
B7_4080589 G/T 7 4080589 GG GG TT TT
B7_4080614 -/+ 7 4080614 - - ++ ++
B7_4080761 A/C 7 4080761 AA AA CC CC
B7_4126649 G/A 7 4126649 GG GG AA GG
B7_4126667 G/A 7 4126667 GG GG AA GG
B7_4126676 G/C 7 4126676 GG GG CC GG
B7_4397366 A/G 7 4397366 AA GG AA AA
B7_4397429 G/T 7 4397429 GG TT GG GG
B7_4397507 T/C 7 4397507 TT CC TT TT
B7_4397513 T/C 7 4397513 TT CC TT TT
B7_4397543 A/G 7 4397543 AA GG AA AA
B7_4423294 +/- 7 4423294 - ++ ++ ++
B7_4489542 A/C 7 4489542 AA AA CC AA
B7_4489618 T/C 7 4489618 TT TT CC TT
B7_4489652 A/G 7 4489652 AA AA GG AA
B7_4542554 T/G 7 4542554 TT TT GG TT
B7_4542592 +/- 7 4542592 ++ ++ - ++
B7_4542614 A/T 7 4542614 AA AA TT AA
B7_4542656 G/A 7 4542656 GG GG AA GG
B7_4687430 T/A 7 4687430 AA TT TT TT
B7_4715097 T/A 7 4715097 AA AA AA TT
B7_4870562 G/A 7 4870562 AA AA GG GG
B7_4870586 +/- 7 4870586 - - ++ ++
B7_5038523 T/C 7 5038523 TT TT CC TT
B7_5098331 A/T 7 5098331 AA AA TT AA
B7_5098619 T/C 7 5098619 TT TT CC TT
B7_5312455 A/G 7 5312455 GG AA AA AA
B7_5312514 A/G 7 5312514 GG AA AA AA
B7_5359124 C/T 7 5359124 TT CC CC CC
B7_5359149 A/C 7 5359149 CC AA AA AA
B7_6755398 T/A 7 6755398 TT TT AA TT
B7_8057983 T/G 7 8057983 TT GT TT TT
B7_8078799 C/G 7 8078799 CC CC GG CC
B7_8854136 T/C 7 8854136 TT TT CC TT B7_8854175 A/G 7 8854175 AA AA GG AA
B7_8871859 T/A 7 8871859 TT TT AA TT
B7_8871868 G/A 7 8871868 GG GG AA GG
B7_8871957 A/T 7 8871957 AA AA TT AA
B7_8871963 A/G 7 8871963 AA AA GG AA
B7_8921956 A/C 7 8921956 AA AC CC AA
B7_8921972 A/G 7 8921972 AA AG GG AA
B7_8921992 G/A 7 8921992 GG AG AA GG
B7_8922001 C/T 7 8922001 CC CT TT CC
B7_8947531 A/G 7 8947531 AA AA GG AA
B7_8947545 T/G 7 8947545 TT TT GG TT
B7_8947569 G/A 7 8947569 GG GG AA GG
B7_8947575 C/A 7 8947575 CC CC AA CC
B7_8952042 T/C 7 8952042 TT TT CC CC
B7_8952056 -/+ 7 8952056 - - ++ ++
B7_8952114 T/C 7 8952114 TT TT CC TT
B7_9134498 C/T 7 9134498 TT CT TT CC
B7_9134524 G/T 7 9134524 GG GT GG TT
B7_9134611 A/T 7 9134611 TT AT TT AA
B7_9134612 G/C 7 9134612 CC GC CC GG
B7_9134634 A/G 7 9134634 GG AG GG AA
B7_9134635 A/C 7 9134635 AA AC AA CC
B7_9134652 A/T 7 9134652 TT AT TT AA
B7_9667888 T/C 7 9667888 CC TT TT TT
B7_9692201 C/T 7 9692201 TT CT CC CC
B7_9931734 C/T 7 9931734 CC CC TT CC
B7_9931788 A/C 7 9931788 AA CC AA AA
B7_9931805 G/A 7 9931805 GG AA AA GG
B7_9931919 T/G 7 9931919 TT GG GG TT
B7_9931935 T/A 7 9931935 TT AA TT TT
B7_9931958 G/A 7 9931958 GG AA GG GG
B7_9931964 T/G 7 9931964 TT GG TT TT
B7_9931970 T/G 7 9931970 TT GG GG TT
B7_9934701 T/G 7 9934701 TT TT TT GG
B7_10617043 A/C 7 10617043 CC AA AA CC
B7_10617052 T/A 7 10617052 AA TT TT AA
B7_10617061 A/C 7 10617061 AA AA AA CC
B7_10670400 T/G 7 10670400 GG GG TT GG
B7_10803311 C/A 7 10803311 AA AA CC AA
B7_10803315 A/G 7 10803315 GG GG AA GG
B7_10803347 T/C 7 10803347 CC CC TT CC B7_10803365 C/T 7 10803365 TT TT CC TT
B7_10868068 T/G 7 10868068 GG GG TT GG
B7_10868070 T/C 7 10868070 CC CC TT CC
B7_10868088 -/+ 7 10868088 ++ ++ - ++
B7_10868106 G/A 7 10868106 AA AA GG AA
B7_10868112 -/+ 7 10868112 ++ ++ - ++
B7_10868173 A/G 7 10868173 GG GG AA GG
B7_11199054 A/T 7 11199054 AA AA TT AA
B7_11626291 A/G 7 11626291 AA AA GG AA
B7_11626344 A/C 7 11626344 AA AA CC AA
B7_13129882 T/C 7 13129882 TT TT TT CC
B7_13664055 C/A 7 13664055 CC CC AA AA
B7_14141321 A/G 7 14141321 AA AA AA GG
B7_14141345 G/C 7 14141345 GG GG GG CC
B7_14141346 T/G 7 14141346 TT TT TT GG
B7_14141374 G/T 7 14141374 GG GG GG TT
B7_14141452 +/- 7 14141452 ++ ++ ++ --
B7_14141457 -/+ 7 14141457 ++ ++ - --
B7_14141464 G/C 7 14141464 GG GG GG CC
B7_14141473 C/T 7 14141473 CC CC CC TT
B7_14256144 C/G 7 14256144 CC CC CC GG
B7_14480799 C/A 7 14480799 AA CC AA AA
B7_14859682 A/G 7 14859682 AA AA GG AA
B7_14859730 C/G 7 14859730 CC CC GG CC
B7_14923947 C/G 7 14923947 CC CC CC GG
B7_14923949 A/G 7 14923949 AA AA GG AA
B7_14923986 A/G 7 14923986 AA AA GG AA
B7_14923994 T/C 7 14923994 TT TT CC TT
B7_14924002 T/C 7 14924002 TT TT CC TT
B7_14924004 +/- 7 14924004 ++ ++ - ++
B7_14924133 T/A 7 14924133 TT TT AA TT
B7_14924154 G/A 7 14924154 GG GG AA GG
B7_14924164 T/C 7 14924164 TT TT CC TT
B7_14924170 C/T 7 14924170 CC CC TT CC
B7_14924173 T/G 7 14924173 TT TT GG TT
B7_14924190 G/T 7 14924190 GG GG TT GG
B7_14924203 A/C 7 14924203 AA AA CC AA
B7_14952611 T/G 7 14952611 GG GG TT TT
B7_14952668 A/G 7 14952668 GG GG AA AA
B7_14970367 A/C 7 14970367 CC CC AA AA
B7_14970397 T/C 7 14970397 CC CC TT TT B7_14970547 +/-/A/G 7 14970547 ++ AA GG GG
B7_14970547 A/G 7 14970547 AA AA GG GG
B7_15617801 +/- 7 15617801 - ++ ++ ++
B7_15789726 C/T 7 15789726 TT CC CC CC
B7_15789729 A/G 7 15789729 GG AA AA AA
B7_15789935 G/A 7 15789935 GG GG GG AA
B7_15789989 C/A 7 15789989 AA CC CC CC
B7_16269278 T/C 7 16269278 TT CC TT CC
B7_16760982 C/A 7 16760982 CC CC AA AA
B7_16761032 G/A 7 16761032 GG GG AA AA
B7_16761042 +/- 7 16761042 ++ ++ - --
B7_16761053 G/C 7 16761053 GG GG CC CC
B7_16761169 T/G 7 16761169 TT TT GG GG
B7_16761220 C/G 7 16761220 CC CC GG GG
B7_16801991 T/G 7 16801991 GG GG TT TT
B7_16802033 A/G 7 16802033 GG GG AA AA
B7_16810563 +/- 7 16810563 - - ++ ++
B7_16810571 +/- 7 16810571 - - ++ ++
B7_17814926 T/G 7 17814926 TT TT TT GG
B7_17814928 T/A 7 17814928 TT TT TT AA
B7_17814937 G/C 7 17814937 GG GG GG CC
B7_17814949 T/A 7 17814949 TT TT TT AA
B7_17982286 A/G 7 17982286 AA GG GG AA
B7_17982342 C/A 7 17982342 CC AA AA CC
B7_17982382 G/A 7 17982382 GG AA AA GG
B7_17982390 G/A 7 17982390 GG AA AA GG
B7_18308131 A/G 7 18308131 AA GG GG AA
B7_18465109 G/C 7 18465109 CC CC CC GG
B7_18797232 T/A 7 18797232 TT AT TT TT
B7_18797293 T/A 7 18797293 TT AT TT TT
B7_19106238 C/A 7 19106238 CC CC AA CC
B7_19106297 A/G 7 19106297 GG AG GG GG
B7_19276190 A/C 7 19276190 CC AC AA CC
B7_19506014 +/- 7 19506014 ++ ++ - ++
B7_19506042 G/C 7 19506042 GG GG CC GG
B7_19506058 -/+ 7 19506058 - - ++ --
B7_19506073 C/T 7 19506073 CC CC TT CC
B7_19518164 C/A 7 19518164 AA AA CC AA
B7_19571295 A/C 7 19571295 AA AA CC AA
B7_19571298 C/T 7 19571298 CC CC TT CC
B7_19571436 A/G 7 19571436 AA AA GG AA B7_20056392 +/- 7 20056392 - ++ ++ ++
B7_21470504 C/A 7 21470504 CC CC AA CC
B7_21623346 T/C 7 21623346 TT TT CC TT
B7_21628835 A/C 7 21628835 AA AA CC AA
B7_21687144 A/C 7 21687144 CC CC AA CC
B7_23248737 G/A 7 23248737 GG GG AA GG
B7_23248748 T/C 7 23248748 TT TT CC TT
B7_23248764 C/G 7 23248764 CC CC GG CC
B7_23248790 +/- 7 23248790 ++ ++ - ++
B7_23248796 G/A 7 23248796 GG GG AA GG
B7_23286433 A/C 7 23286433 AA AA CC AA
B7_23286467 C/G 7 23286467 CC CC GG CC
B7_23286491 C/A 7 23286491 CC CC AA CC
B7_23286492 A/C 7 23286492 AA AA CC CC
B7_23286524 C/T 7 23286524 CC CC CC TT
B7_23295627 A/C 7 23295627 AA AA CC AA
B7_23295643 G/A 7 23295643 GG GG AA GG
B7_23295690 A/T 7 23295690 AA AA TT AA
B7_23338560 G/A 7 23338560 GG GG AA GG
B7_23338582 G/C 7 23338582 GG GG CC GG
B7_23338627 C/T 7 23338627 CC CC TT CC
B7_23338664 A/G 7 23338664 AA AA GG AA
B7_23338692 T/G 7 23338692 TT TT GG TT
B7_23937818 T/C 7 23937818 TT TT CC TT
B7_23937835 C/A 7 23937835 CC CC AA CC
B7_23937863 G/C 7 23937863 GG GG CC GG
B7_23937921 C/T 7 23937921 CC CC TT CC
B7_23937928 C/T 7 23937928 CC CC TT CC
B7_23937929 A/G 7 23937929 AA AA GG AA
B7_23937935 C/T 7 23937935 CC CC TT CC
B7_23937958 C/T 7 23937958 CC CC TT CC
B7_23938008 C/T 7 23938008 CC CC TT CC
B7_23938009 C/T 7 23938009 CC CC TT CC
B7_23938027 G/A 7 23938027 GG GG AA GG
B7_23943359 C/T 7 23943359 CC CC TT CC
B7_23943384 +/- 7 23943384 ++ ++ - ++
B7_23943394 -/+ 7 23943394 - - ++ --
B7_23943402 T/C 7 23943402 TT TT CC TT
B7_23943420 A/G 7 23943420 AA AA GG AA
B7_23943829 A/C 7 23943829 AA AA CC AA
B7_23943942 A/C 7 23943942 AA AA CC AA B7_23943953 G/C 7 23943953 GG GG CC GG
B7_23944021 C/A 7 23944021 CC CC AA CC
B7_23944026 G/A 7 23944026 GG GG AA GG
B7_23944055 C/T 7 23944055 CC CC TT CC
B7_23944225 G/A 7 23944225 GG GG AA GG
B7_23945014 C/A 7 23945014 CC CC AA CC
B7_23945040 G/T 7 23945040 GG GG TT GG
B7_23945046 T/A 7 23945046 TT TT AA TT
B7_23945062 A/T 7 23945062 AA AA TT AA
B7_23945069 A/G 7 23945069 AA AA GG AA
B7_23945078 A/G 7 23945078 AA AA GG AA
B7_24752771 +/- 7 24752771 - ++ ++ ++
B7_25095308 C/T 7 25095308 CC CT CC TT
B7_28711224 +/- 7 28711224 - ++ ++ ++
B7_28938294 +/- 7 28938294 - ++ - ++
B7_30061285 C/T 7 30061285 CC CT TT TT
B7_30171387 A/G 7 30171387 AA AG GG GG
B7_30171390 A/G 7 30171390 AA AG GG GG
B7_30171528 T/C 7 30171528 TT CC CC CC
B7_30299357 C/G 7 30299357 CC GC GG GG
B7_30299471 T/C 7 30299471 TT CT CC CC
B7_30359568 A/G 7 30359568 AA AG GG GG
B7_30507519 A/G 7 30507519 AA AA GG GG
B7_30507527 A/T 7 30507527 AA AA TT TT
B7_30507529 T/G 7 30507529 TT TT GG GG
B7_30529097 -/+ 7 30529097 - - ++ ++
B7_30529101 +/- 7 30529101 ++ ++ - --
B7_30529157 T/C 7 30529157 TT TT CC CC
B7_30567752 G/A 7 30567752 GG GG AA AA
B7_30617261 -/+ 7 30617261 - - ++ ++
B7_30617446 A/T 7 30617446 AA TT TT TT
B7_31453579 G/A 7 31453579 GG GG AA AA
B7_32286479 G/C 7 32286479 CC GG GG GG
B7_32329936 T/A 7 32329936 AA TT TT TT
B7_32564354 C/T 7 32564354 TT CC CC CC
B7_32564409 T/A 7 32564409 TT AA AA AA
B7_32564410 +/- 7 32564410 - ++ ++ ++
B7_32576578 C/A 7 32576578 CC CC AA AA
B7_32576691 A/G 7 32576691 AA AA GG GG
B7_32959461 T/C 7 32959461 TT CT CC CC
B7_32959475 C/G 7 32959475 GG GG CC CC B7_32959510 G/A 7 32959510 GG GG AA AA
B7_32986198 T/G 7 32986198 GG GT TT TT
B7_32986326 G/C 7 32986326 GG GC CC CC
B7_33125929 T/G 7 33125929 TT GT GG GG
B7_33316655 T/C 7 33316655 CC TT TT TT
B7_33316728 T/C 7 33316728 TT CT CC CC
B7_33316733 C/T 7 33316733 CC CT TT TT
B7_33549268 +/- 7 33549268 - -+ ++ ++
B7_33549355 C/A 7 33549355 CC AC AA AA
B7_33606776 T/G 7 33606776 TT GG GG GG
B7_35065335 C/T 7 35065335 CC CC TT CC
B7_36312495 +/- 7 36312495 - -+ - --
B7_36677936 +/- 7 36677936 - ++ ++ --
B7_36677937 G/A 7 36677937 GG GG GG AA
B7_38482189 T/A 7 38482189 TT TT AA TT
B7_38482206 C/T 7 38482206 CC CC TT CC
B7_38482208 G/T 7 38482208 GG GG TT GG
B7_38482386 G/C 7 38482386 GG GG CC GG
B7_38510680 G/T 7 38510680 GG GG TT GG
B7_38544790 G/A 7 38544790 GG GG AA GG
B7_38608571 T/C 7 38608571 TT TT CC TT
B7_39544568 T/C 7 39544568 TT CT CC CC
B7_39544626 T/A 7 39544626 TT AT AA AA
B7_39544697 T/C 7 39544697 TT CT CC CC
B7_39544699 G/A 7 39544699 GG AG AA AA
B7_39544708 T/C 7 39544708 TT CT CC CC
B7_39544739 T/A 7 39544739 TT AT AA AA
B7_39679990 T/C 7 39679990 TT CT CC CC
B7_39680132 A/C 7 39680132 AA AC CC CC
B7_39786723 T/A 7 39786723 TT AA AA AA
B7_39786731 C/T 7 39786731 CC TT TT TT
B7_39786732 T/C 7 39786732 TT CC CC CC
B7_39833593 A/G 7 39833593 AA AA GG GG
B7_39833599 G/C 7 39833599 GG GG CC CC
B7_39926711 C/G 7 39926711 CC CC CC GG
B7_39929055 C/T 7 39929055 CC CC TT TT
B7_39971210 A/C 7 39971210 AA AA CC CC
B7_39971317 T/C 7 39971317 TT TT CC CC
B7_39971318 -/+ 7 39971318 - - ++ ++
B7_40060721 C/A 7 40060721 CC CC AA CC
B7_40074046 +/- 7 40074046 ++ ++ - ++ B7_40160968 A/G 7 40160968 AA AA GG AA
B7_40275617 A/G 7 40275617 AA AA GG AA
B7_40275618 A/C 7 40275618 AA AA CC AA
B7_40275663 C/A 7 40275663 CC CC AA CC
B7_40292787 G/A 7 40292787 GG GG AA GG
B7_40292887 C/T 7 40292887 CC CC TT CC
B7_40299802 G/T 7 40299802 GG GG TT GG
B7_40299895 -/+ 7 40299895 - - ++ --
B7_40308278 G/A 7 40308278 GG GG AA GG
B7_40684679 G/A 7 40684679 GG GG AA GG
B7_41161522 A/T 7 41161522 AA AA TT AA
B7_41161532 C/A 7 41161532 CC CC AA CC
B7_41161573 A/G 7 41161573 AA AA GG AA
B7_41252423 A/C 7 41252423 AA AA CC AA
B7_41252425 -/+ 7 41252425 - - ++ --
B7_41270977 T/A 7 41270977 TT TT AA TT
B7_41270979 G/T 7 41270979 GG GG TT GG
B7_41271071 C/G 7 41271071 CC CC GG CC
B7_41271086 C/T 7 41271086 CC CT CC TT
Ta j/e 25: SWP one nrfe/ markers on carinata chromosome B8.
B8_3138586 A/G 8 3138586 AA AA GG AA
B8_3138589 A/G 8 3138589 AA AA GG AA
B8_3138628 A/G 8 3138628 AA AA GG AA
B8_3138636 C/G 8 3138636 CC CC GG CC
B8_3138695 G/C 8 3138695 GG GG CC GG
B8_3138696 C/T 8 3138696 CC CC TT CC
B8_3186949 A/C 8 3186949 CC CC AA AA
B8_3191268 G/A 8 3191268 AA AA GG GG
B8_3191352 G/A 8 3191352 AA AA GG GG
B8_3191405 C/T 8 3191405 TT TT CC CC
B8_3370666 A/G 8 3370666 AA AA GG GG
B8_4133264 G/A 8 4133264 AA AA GG GG
B8_4133271 T/C 8 4133271 CC CC TT TT
B8_4133289 C/G 8 4133289 GG GG CC CC
B8_4133310 G/A 8 4133310 AA AA GG GG
B8_4133337 A/G 8 4133337 GG GG AA AA
B8_4133598 C/A 8 4133598 AA AA CC CC
B8_4165900 T/A 8 4165900 AA AA TT TT
B8_4595200 G/T 8 4595200 GG TT TT TT
B8_4595354 G/T 8 4595354 GG TT TT TT
B8_4595359 A/T 8 4595359 AA TT TT TT
B8_4941194 T/A 8 4941194 AA AA TT TT
B8_4948345 A/G 8 4948345 AA AA GG GG
B8_4948918 A/G 8 4948918 AA AA GG GG
B8_4948947 C/G 8 4948947 CC CC GG GG
B8_6002014 G/A 8 6002014 GG GG AA AA
B8_6002027 +/- 8 6002027 - ++ ++ ++
B8_6244035 T/G 8 6244035 GG GG TT TT
B8_6244045 G/A 8 6244045 AA AA GG GG
B8_6244225 G/A 8 6244225 AA AA GG GG
B8_6244238 C/T 8 6244238 TT TT CC CC
B8_6347021 T/A 8 6347021 TT TT AA AA
B8_6347244 C/T 8 6347244 CC CC TT TT
B8_6347257 C/G 8 6347257 CC CC GG GG
B8_6347268 A/C 8 6347268 AA AA CC CC
B8_6362212 T/G 8 6362212 TT TT GG GG
B8_6362337 C/A 8 6362337 CC CC AA AA
B8_6362359 C/T 8 6362359 CC CC TT TT
B8_6417857 A/T 8 6417857 AA AA TT TT
B8_6417860 +/- 8 6417860 ++ ++ -
B8_6418001 G/A 8 6418001 GG GG AA AA B8_7529862 G/A 8 7529862 GG GG AA AA
B8_7529866 A/G 8 7529866 AA AA GG GG
B8_7529890 A/T 8 7529890 AA AA TT TT
B8_7529924 T/C 8 7529924 TT TT CC TT
B8_7530033 G/A 8 7530033 GG GG AA AA
B8_7530039 G/T 8 7530039 GG GG TT TT
B8_7530068 C/T 8 7530068 CC CC TT TT
B8_7705274 G/A 8 7705274 GG GG AA AA
B8_7705306 A/G 8 7705306 AA AA GG GG
B8_7705569 A/C 8 7705569 AA AA CC CC
B8_7718880 G/A 8 7718880 GG GG AA AA
B8_8391973 C/A 8 8391973 CC CC AA AA
B8_8392009 C/T 8 8392009 CC CC TT TT
B8_8392012 C/A 8 8392012 CC CC AA AA
B8_8392018 G/A 8 8392018 GG AG AA AA
B8_8392071 T/C 8 8392071 TT TT CC CC
B8_8494304 A/G 8 8494304 AA AA GG GG
B8_9935447 A/G 8 9935447 AA AA GG GG
B8_9935450 T/G 8 9935450 TT TT GG GG
B8_9935465 A/G 8 9935465 AA AA GG GG
B8_9982889 -/+ 8 9982889 - - ++ ++
B8_9982908 A/C 8 9982908 AA AA CC CC
B8_10012478 A/G 8 10012478 AA AA GG GG
B8_10012481 A/G 8 10012481 AA AA GG GG
B8_11321729 +/- 8 11321729 - ++ ++ ++
B8_12164698 A/G 8 12164698 GG GG AA AA
B8_12164707 T/A 8 12164707 AA AA TT TT
B8_12243614 G/A 8 12243614 AA AA GG GG
B8_12268285 T/G 8 12268285 GG GG TT TT
B8_12268293 -/+ 8 12268293 ++ ++ - --
B8_12268299 A/T 8 12268299 TT TT AA AA
B8_12268320 -/+ 8 12268320 ++ ++ - --
B8_12268406 A/C 8 12268406 CC CC AA AA
B8_12268451 C/T 8 12268451 TT TT CC CC
B8_12553358 C/A 8 12553358 AA AA CC CC
B8_12553376 G/T 8 12553376 TT TT GG GG
B8_12553381 T/A 8 12553381 AA AA TT TT
B8_12577397 G/T 8 12577397 TT TT GG GG
B8_12577626 C/A 8 12577626 AA AA CC CC
B8_14016390 A/G 8 14016390 AA AA GG GG
B8_14016489 T/C 8 14016489 TT TT CC CC B8_14017343 A/T 8 14017343 AA AA TT TT
B8_14643054 A/G 8 14643054 AA AA GG GG
B8_14643087 G/A 8 14643087 GG GG AA AA
B8_15100151 G/T 8 15100151 GG GG TT TT
B8_15560051 T/C 8 15560051 TT TT CC CC
B8_15560057 C/T 8 15560057 CC CC TT TT
B8_15789598 +/- 8 15789598 - ++ ++ --
B8_16792895 G/A 8 16792895 AA AA GG GG
B8_18048421 A/C 8 18048421 AA AA CC CC
B8_18048471 C/T 8 18048471 CC CC TT TT
B8_18048632 C/A 8 18048632 CC CC AA AA
B8_18123926 G/T 8 18123926 GG GG TT TT
B8_18313384 -/+ 8 18313384 - - ++ ++
B8_18339707 C/T 8 18339707 CC CC TT TT
B8_18339712 C/G 8 18339712 CC CC GG GG
B8_18339748 C/T 8 18339748 CC CC TT TT
B8_19149989 +/- 8 19149989 - ++ ++ ++
B8_22821928 +/- 8 22821928 - ++ ++ ++
B8_22990316 T/C 8 22990316 CC CC TT CC
B8_22990325 T/A 8 22990325 AA AA TT AA
B8_23239481 +/- 8 23239481 - ++ ++ ++
B8_23476512 G/A 8 23476512 AA GG GG AA
B8_23521887 +/- 8 23521887 - ++ ++ --
B8_23799163 T/A 8 23799163 AA AA TT AA
B8_23799201 A/C 8 23799201 CC CC AA CC
B8_23846657 G/T 8 23846657 TT TT GG TT
B8_24822175 G/A 8 24822175 GG GG AA AA
B8_24822309 C/T 8 24822309 CC CC TT TT
B8_24894427 +/- 8 24894427 - ++ ++ ++
B8_28440104 T/C 8 28440104 CC CC TT TT
B8_28484898 G/C 8 28484898 GG GG CC CC
B8_29423999 T/G 8 29423999 TT TT GG TT
B8_30988629 +/- 8 30988629 - ++ ++ ++
B8_32727120 G/T 8 32727120 GG GG TT GG
B8_32900417 G/A 8 32900417 AA AA GG GG
B8_35916210 +/- 8 35916210 - ++ ++ ++
B8_36057952 +/- 8 36057952 - - ++ ++
B8_39079317 G/A 8 39079317 GG GG GG AA
B8_42731470 +/- 8 42731470 - - ++ ++
B8_42742470 +/- 8 42742470 - - ++ ++
B8_44851959 A/G 8 44851959 GG GG AA AA B8_44852066 A/T 8 44852066 TT TT AA AA
B8_44988030 T/C 8 44988030 CC CC TT TT
B8_44988044 A/G 8 44988044 GG GG AA AA
B8_45665359 A/G 8 45665359 AA AA AA GG
B8_45709506 C/A 8 45709506 CC CC AA AA
B8_45709541 G/A 8 45709541 GG GG AA AA
B8_45709739 C/T 8 45709739 CC CC TT TT
B8_46319592 T/G 8 46319592 TT TT TT GG
B8_46323052 +/- 8 46323052 ++ ++ - ++
B8_46323053 +/- 8 46323053 ++ ++ - ++
B8_46323054 +/- 8 46323054 ++ ++ - ++
B8_46323075 G/A 8 46323075 GG GG GG AA
B8_46384491 T/C 8 46384491 TT TT CC CC
B8_46467259 A/G 8 46467259 AA AA GG GG
B8_47148213 G/T 8 47148213 GG GG GG TT
B8_47148263 G/A 8 47148263 GG GG GG AA
B8_47148382 C/T 8 47148382 CC CC TT CC
B8_47254049 G/A 8 47254049 GG GG GG AA
B8_47491185 A/G 8 47491185 AA AA AA GG
B8_48103381 G/C 8 48103381 GG GG CC GG
B8_48103392 A/C 8 48103392 AA AA CC AA
B8_48103494 A/G 8 48103494 AA AA GG AA
B8_48144356 +/- 8 48144356 ++ ++ - ++
B8_49616122 T/C 8 49616122 TT TT TT CC
B8_49616208 C/T 8 49616208 CC CC CC TT
B8_49831698 G/A 8 49831698 GG GG GG AA
B8_49831746 -/+ 8 49831746 - - - ++
B8_49831749 T/G 8 49831749 TT TT TT GG
B8_49831895 C/T 8 49831895 CC CC CC TT
B8_49900066 G/A 8 49900066 GG GG GG AA
B8_49900202 +/- 8 49900202 ++ ++ ++ --
B8_49900244 A/C 8 49900244 AA AA AA CC
B8_49900247 A/G 8 49900247 AA AA AA GG
B8_51057417 A/G 8 51057417 GG AA AA AA
B8_52088117 T/C 8 52088117 CC CC TT TT
B8_52088119 A/G 8 52088119 GG GG AA AA
B8_52088167 G/A 8 52088167 AA AA GG GG
B8_52088170 C/T 8 52088170 TT TT CC CC
B8_52088326 G/T 8 52088326 TT TT GG GG
B8_52727370 G/A 8 52727370 GG GG AA AA
B8_52727373 G/A 8 52727373 GG GG AA AA B8_52727394 +/- 8 52727394 ++ ++ -
B8_52727398 +/- 8 52727398 ++ ++ -
B8_52727570 C/T 8 52727570 CC CC TT TT
B8_52737883 G/A 8 52737883 GG GG AA AA
B8_52737912 C/T 8 52737912 CC CC TT TT
B8_53493020 C/G 8 53493020 GG GG CC GG
B8_54089490 T/C 8 54089490 TT TT CC TT
B8_54089574 G/A 8 54089574 GG GG AA GG
B8_54089622 C/T 8 54089622 CC CC TT CC
B8_54089625 C/T 8 54089625 CC CC TT CC
Table 26: SNP and indel markers on carinata chromosome CI.
Cl_7481868 -/+ 9 7481868 - -- ++ -
Cl_7973344 G/T 9 7973344 TT TT GG TT
Cl_7973404 G/C 9 7973404 GG GG CC GG
Cl_8047087 G/A 9 8047087 AA AA AA AA
Cl_8329614 A/G 9 8329614 AA AA AA GG
Cl_8995679 C/G 9 8995679 CC CC CC GG
Cl_8995722 G/T 9 8995722 GG GG GG TT
Cl_9075842 A/C 9 9075842 CC CC AA AA
Cl_10535408 T/C 9 10535408 TT CC TT CC
Cl_11205784 G/C 9 11205784 GG CC GG GG
Cl_11214491 -/+ 9 11214491 ++ ++ - -
Cl_11214494 G/A 9 11214494 AA AA GG GG
Cl_11214602 T/G 9 11214602 TT GG TT TT
Cl_11238865 C/T 9 11238865 CC TT CC CC
Cl_11340925 C/T 9 11340925 CC CC CC TT
Cl_11341081 G/A 9 11341081 GG GG GG AA
Cl_11342007 T/C 9 11342007 TT TT TT CC
Cl_11342070 A/C 9 11342070 AA AA AA CC
Cl_11656283 G/A 9 11656283 AA GG AA AA
Cl_11656371 T/C 9 11656371 CC TT CC CC
Cl_11879045 +/- 9 11879045 - -- ++ ++
Cl_13213148 A/C 9 13213148 AA CC AA AA
Cl_13213168 -/+ 9 13213168 - ++ - -
Cl_13213170 +/- 9 13213170 ++ -- ++ ++
Cl_13607542 G/C 9 13607542 GG CC GG GG
Cl_14274629 G/A 9 14274629 GG AA GG GG
Cl_14274642 T/C 9 14274642 TT CC TT TT
Cl_14391249 +/- 9 14391249 - ++ - ++
Cl_15058347 A/G 9 15058347 AA GG AA GG
Cl_15091576 T/C 9 15091576 TT CC TT CC
Cl_15091577 T/C 9 15091577 TT CC TT CC
Cl_15146165 G/A 9 15146165 GG AA GG AA
Cl_15146199 G/A 9 15146199 GG AA GG AA
Cl_15146206 G/A 9 15146206 GG AA GG AA
Cl_15146212 A/G 9 15146212 AA GG AA GG
Cl_15146243 A/G 9 15146243 AA GG AA GG
Cl_15154306 G/A 9 15154306 GG AA GG AA
Cl_15198584 C/T 9 15198584 CC TT CC TT
Cl_15198611 T/C 9 15198611 TT CC TT CC
Cl_15198635 C/T 9 15198635 CC TT CC TT
Cl_15198646 G/A 9 15198646 GG AA GG AA Cl_15753817 A/G 9 15753817 AA GG AA GG
Cl_15754498 A/T 9 15754498 AA TT AA TT
Cl_15855165 A/G 9 15855165 AA GG AA AA
Cl_17184379 A/G 9 17184379 GG AA AA AA
Cl_24823670 +/- 9 24823670 - ++ ++ ++
Cl_25812877 T/C 9 25812877 TT TT TT CC
Cl_28763144 +/- 9 28763144 - ++ ++ ++
Cl_33744223 A/G 9 33744223 AA AA AA GG
Cl_33744234 C/T 9 33744234 CC CC CC TT
Cl_33744240 C/A 9 33744240 CC CC CC AA
Cl_33744303 G/A 9 33744303 GG GG GG AA
Cl_33795936 A/C 9 33795936 AA AA AA CC
Cl_33796068 C/T 9 33796068 CC CC CC TT
Cl_35710691 G/T 9 35710691 GG GG GG TT
Cl_35835246 G/A 9 35835246 GG GG GG AA
Cl_40664205 G/A 9 40664205 GG GG AA AA
Cl_40941118 T/G 9 40941118 TT TT GG TT
Cl_40941172 T/C 9 40941172 TT TT CC TT
Cl_41471413 C/A 9 41471413 CC AA AA AA
Cl_41471437 G/C 9 41471437 GG CC CC CC
Cl_41648625 +/- 9 41648625 - ++ ++
Cl_42587841 T/C 9 42587841 CC CC TT TT
Cl_42587876 A/G 9 42587876 GG GG AA AA
Cl_42587881 G/C 9 42587881 CC CC GG GG
Cl_42587918 A/G 9 42587918 GG GG AA AA
Cl_42730669 A/G 9 42730669 AA AA AA GG
Cl_42730813 G/C 9 42730813 GG GG GG CC
Table 27: SNP and indel markers on carinata chromosome C2. C2_2759828 A/G 10 2759828 GG AA GG GG
C2_2759842 G/T 10 2759842 TT GG TT TT
C2_2836709 T/A 10 2836709 AA TT TT TT
C2_2991663 T/A 10 2991663 TT AA AA AA
C2_3663311 C/T 10 3663311 CC TT CC CC
C2_3670932 G/A 10 3670932 GG AA GG GG
C2_4276920 G/A 10 4276920 GG GG AA GG
C2_4312969 A/G 10 4312969 AA AA GG AA
C2_4313001 T/A 10 4313001 TT TT AA TT
C2_4904459 C/T 10 4904459 CC TT CC CC
C2_4904495 G/A 10 4904495 GG AA GG GG
C2_4904504 A/C 10 4904504 AA CC AA AA
C2_4904610 C/T 10 4904610 CC TT CC CC
C2_4904627 C/G 10 4904627 CC GG CC CC
C2_5345920 T/C 10 5345920 TT CC TT TT
C2_5345935 A/G 10 5345935 AA GG AA AA
C2_5346054 C/T 10 5346054 CC TT CC CC
C2_6992132 T/C 10 6992132 TT CC TT TT
C2_7037297 G/A 10 7037297 GG AA GG GG
C2_7037413 A/G 10 7037413 AA GG AA AA
C2_7037447 C/G 10 7037447 CC GG CC CC
C2_7037455 C/T 10 7037455 CC TT CC CC
C2_7038868 A/C 10 7038868 AA CC AA AA
C2_14184685 +/- 10 14184685 - ++ ++
C2_15228924 G/T/A 10 15228924 GG GG TT GG
C2_15385104 +/- 10 15385104 - ++ -
C2_21439079 A/G 10 21439079 GG AA GG AA
C2_28463898 C/T 10 28463898 CC CC TT TT
C2_29475511 C/G 10 29475511 CC CC CC GG
C2_35663725 +/- 10 35663725 - ++ ++
C2_36486176 C/T 10 36486176 CC TT CC CC
C2_42185103 +/- 10 42185103 - ++ ++ -
C2_45008084 10 45008084 - ++ ++
C2_45501881 +/- 10 45501881 - ++ ++
C2_47679077 +/- 10 47679077 - ++ ++ -
C2_49092619 G/T 10 49092619 GG GT GG GG
C2_49092678 T/C 10 49092678 TT CT TT TT Table 28: SNP and indel markers on carinata chromosome C3.
C3_10037134 T/C 11 10037134 TT TT CC TT
C3_10048139 C/G 11 10048139 CC CC GG CC
C3_10520990 G/A 11 10520990 GG AA AA GG
C3_10918852 G/T/A 11 10918852 GG GG TT GG
C3_12863214 C/T 11 12863214 CC TT CC CC
C3_14810500 A/T 11 14810500 TT TT AA AA
C3_14810677 A/G 11 14810677 GG GG AA AA
C3_15602161 C/T 11 15602161 CC TT TT TT
C3_15602163 A/C 11 15602163 AA CC CC CC
C3_15602216 T/C 11 15602216 TT CC CC CC
C3_15930324 G/C 11 15930324 CC GG GG GG
C3_15930354 +/- 11 15930354 ++ ++ ++
C3_15939828 T/A 11 15939828 AA TT TT TT
C3_17177929 C/A 11 17177929 AA CC AA AA
C3_17178063 11 17178063 ++ ++ -
C3_17178120 G/C 11 17178120 CC GG CC CC
C3_17178249 G/A 11 17178249 AA GG AA GG
C3_17203544 A/G 11 17203544 GG AA GG GG
C3_17216383 C/A 11 17216383 AA CC AA CC
C3_17440059 C/A 11 17440059 CC CC AA AA
C3_18312272 A/G 11 18312272 GG AA GG GG
C3_18312296 T/A 11 18312296 AA TT AA AA
C3_18312303 G/A 11 18312303 AA GG AA AA
C3_18987061 +/- 11 18987061 ++ ++ ++
C3_21472822 G/A 11 21472822 GG GG AA AA
C3_21690969 +/- 11 21690969 ++ ++ ++
C3_22569196 G/A 11 22569196 AA GG GG GG
C3_22569199 G/A 11 22569199 AA GG GG GG
C3_22569202 T/C 11 22569202 CC TT TT TT
C3_22569226 A/G 11 22569226 GG AA AA AA
C3_22569250 +/- 11 22569250 ++ ++ ++
C3_23451713 A/G 11 23451713 AA GG AA AA
C3_23451753 T/C 11 23451753 TT CC TT TT
C3_23451962 G/T 11 23451962 GG TT GG GG
C3_23452086 A/G 11 23452086 AA GG AA AA
C3_24195209 C/T 11 24195209 CC TT TT TT
C3_24195215 C/G 11 24195215 CC GG GG GG
C3_24195238 G/A 11 24195238 GG AA AA AA
C3_24195265 11 24195265 ++ ++ ++
C3_24195273 +/- 11 24195273 ++ - -
C3_24195278 A/T 11 24195278 AA TT TT TT C3_24195282 +/- 11 24195282 ++ - -
C3_24195283 +/- 11 24195283 ++ - -
C3_24195311 11 24195311 ++ ++ ++
C3_25029585 +/- 11 25029585 ++ ++ ++
C3_25957548 G/A 11 25957548 GG GG AA AA
C3_25957577 C/G 11 25957577 CC CC GG GG
C3_25957598 A/G 11 25957598 AA AA GG GG
C3_27064045 C/T 11 27064045 CC TT CC CC
C3_29046232 T/G 11 29046232 TT TT GG GG
C3_29046278 G/A 11 29046278 GG GG AA AA
C3_29046307 G/T 11 29046307 GG GG TT TT
C3_29046388 C/T 11 29046388 CC CC TT TT
C3_29046395 A/C 11 29046395 AA AA CC CC
C3_29754400 A/C 11 29754400 AA AA CC CC
C3_29754430 C/T 11 29754430 TT CC CC CC
C3_31586079 T/C 11 31586079 CC TT CC CC
C3_31586707 G/T 11 31586707 TT GG TT TT
C3_31631989 T/A 11 31631989 AA TT AA AA
C3_31632035 A/G 11 31632035 GG AA GG GG
C3_31632048 T/G 11 31632048 GG TT GG GG
C3_31712163 G/T 11 31712163 TT GG TT TT
C3_31713397 T/C 11 31713397 TT CC TT TT
C3_31713398 T/A 11 31713398 TT AA TT TT
C3_32992766 C/T 11 32992766 TT CC CC CC
C3_34884215 A/C 11 34884215 CC CC AA AA
C3_34907914 G/A 11 34907914 AA AA GG GG
C3_34907920 A/C 11 34907920 CC CC AA AA
C3_35611604 A/T 11 35611604 AA TT TT TT
C3_35611645 T/C 11 35611645 TT CC CC CC
C3_35611673 G/A 11 35611673 GG AA AA AA
C3_36344095 C/T 11 36344095 CC TT CC CC
C3_36344107 11 36344107 ++ - -
C3_36362939 A/G 11 36362939 AA GG AA AA
C3_36375157 +/- 11 36375157 ++ ++ ++
C3_36836893 C/T 11 36836893 CC CC TT TT
C3_36991672 C/T 11 36991672 TT TT CC CC
C3_37772500 C/G 11 37772500 CC GG GG GG
C3_37772623 G/A 11 37772623 GG AA AA AA
C3_42991081 +/- 11 42991081 ++ ++ -
C3_44468558 +/- 11 44468558 ++ ++ ++
C3_45818710 +/- 11 45818710 ++ ++ - C3_51753322 A/C 11 51753322 AA AA CC AA
C3_51983708 C/A 11 51983708 AA AA CC CC
C3_51983742 C/T 11 51983742 TT TT CC CC
C3_51983757 C/T 11 51983757 TT TT CC CC
C3_52950507 C/T 11 52950507 CC TT CC CC
C3_52965590 T/C 11 52965590 TT CC TT TT
C3_52965690 C/A 11 52965690 CC AA CC CC
C3_52965696 T/G 11 52965696 TT GG TT TT
C3_55767842 C/A 11 55767842 AA CC CC CC
C3_56855879 G/T 11 56855879 GG TT GG GG
C3_56855941 C/A 11 56855941 CC AA CC CC
C3_56859509 C/T 11 56859509 CC TT CC CC
C3_56859547 A/G 11 56859547 AA GG AA AA
C3_56859587 A/C 11 56859587 AA CC AA AA
C3_57142241 A/G 11 57142241 GG GG AA AA
C3_57142510 G/T 11 57142510 TT TT GG GG
C3_58071700 G/A 11 58071700 AA AA GG GG
C3_58071768 A/G 11 58071768 GG GG AA AA
C3_58323349 G/A 11 58323349 AA AA GG GG
C3_61193980 +/- 11 61193980 -- ++ ++ -
C3_62100550 T/C 11 62100550 CC TT TT TT
C3_62111641 C/T 11 62111641 CC CC TT TT
C3_62111674 T/C 11 62111674 TT TT CC CC
C3_63656665 C/A 11 63656665 CC CC AA CC
C3_63656747 C/T 11 63656747 CC CC TT TT
C3_64263357 A/C 11 64263357 CC AA AA AA
C3_64263365 A/G 11 64263365 GG AA AA AA
C3_64263380 T/A 11 64263380 AA TT TT TT
C3_64471086 A/C 11 64471086 CC AA AA AA
Table 29: SNP and indel markers on carinata chromosome C4. C4_1763854 T/A 12 1763854 TT AA AA AA
C4_1763920 G/A 12 1763920 GG AA AA AA
C4_1770658 T/C 12 1770658 TT CT TT TT
C4_1841557 +/- 12 1841557 - ++ ++ -
C4_2744194 T/A 12 2744194 AT AT AA AA
C4_3358344 T/C 12 3358344 TT CT TT TT
C4_5594359 G/A 12 5594359 GG GG GG AA
C4_5867296 T/A 12 5867296 TT AA AA TT
C4_5867309 G/A 12 5867309 GG AA AA GG
C4_5936991 G/A 12 5936991 GG AG AA GG
C4_5937000 T/C 12 5937000 TT CT CC TT
C4_5937005 +/- 12 5937005 ++ -+ - ++
C4_6891288 A/T 12 6891288 TT AA AA AA
C4_7065484 C/A 12 7065484 cc AA AA AA
C4_7065525 C/A 12 7065525 cc AA AA AA
C4_7065575 A/C 12 7065575 AA CC CC CC
C4_7065577 G/T 12 7065577 GG TT TT TT
C4_7065591 C/A 12 7065591 CC AA AA AA
C4_7313310 T/C 12 7313310 TT CT CC CC
C4_9060496 G/A 12 9060496 GG AG GG GG
C4_9880986 C/T 12 9880986 CC CC CC TT
C4_9880995 T/A 12 9880995 TT TT TT AA
C4_9881027 T/A 12 9881027 TT TT TT AA
C4_10126279 G/A 12 10126279 GG GG GG AA
C4_10126285 A/T 12 10126285 AA AA AA TT
C4_10126326 G/A 12 10126326 GG GG GG AA
C4_10152940 A/T 12 10152940 AA AA AA TT
C4_10153067 T/A 12 10153067 TT TT TT AA
C4_10153111 G/A 12 10153111 GG GG GG AA
C4_10888972 G/A 12 10888972 GG GG AA GG
C4_10995930 T/A 12 10995930 TT TT AA TT
C4_16680120 -/+ 12 16680120 - -- - ++
C4_21789986 +/- 12 21789986 - ++ ++ -
C4_22725587 +/- 12 22725587 - ++ ++ -
C4_27450819 T/G 12 27450819 GG TT GG TT
C4_32528514 G/A 12 32528514 AA AA AA GG
C4_32528517 C/G 12 32528517 GG GG GG CC
C4_33927249 T/G 12 33927249 TT TT GG GG
C4_37868619 C/A 12 37868619 CC CC CC AA
C4_37868763 G/A 12 37868763 GG GG GG AA
C4_37868766 G/T 12 37868766 GG GG GG TT C4_37985818 C/T 12 37985818 CC CC CC TT
C4_38052425 T/C 12 38052425 TT TT TT CC
C4_38052612 G/C 12 38052612 GG GG GG CC
C4_38114258 G/A 12 38114258 GG GG GG AA
C4_38135264 G/A 12 38135264 GG GG GG AA
C4_38135266 C/A 12 38135266 CC CC CC AA
C4_38135330 A/G 12 38135330 AA AA AA GG
C4_38135333 T/C 12 38135333 TT TT TT CC
C4_38149116 C/T 12 38149116 CC CC CC TT
C4_38149201 T/C 12 38149201 TT TT TT CC
C4_38748104 C/T 12 38748104 TT TT TT CC
C4_39340740 +/- 12 39340740 - ++ ++ ++
C4_40489982 C/T 12 40489982 CC CC TT CC
C4_40700520 A/T 12 40700520 AA AA TT TT
C4_40729697 C/T 12 40729697 CC CT CC CC
C4_42120786 C/T 12 42120786 CC CC TT TT
C4_42399184 +/- 12 42399184 - ++ ++ ++
C4_42476790 C/A 12 42476790 CC CC CC AA
C4_44105942 C/G 12 44105942 GG GG CC GG
C4_45069785 A/T 12 45069785 TT TT TT AA
C4_45069866 C/G 12 45069866 GG GG GG CC
C4_45547283 A/G 12 45547283 AA AA GG AA
C4_45632158 C/T 12 45632158 CC CC TT TT
C4_45632227 G/A 12 45632227 AA AA GG GG
C4_45632323 G/C 12 45632323 GG GG GG CC
C4_45632341 T/G 12 45632341 TT TT TT GG
C4_45632344 C/T 12 45632344 CC CC CC TT
C4_45632353 T/G 12 45632353 TT TT TT GG
C4_49259378 T/G 12 49259378 TT TT GG TT
C4_52861602 G/A 12 52861602 GG GG AA AA
C4_53426635 C/T 12 53426635 TT TT CC CC
C4_53426676 G/A 12 53426676 AA AA GG GG
C4_53426689 T/C 12 53426689 CC CC TT TT
C4_53426737 T/A 12 53426737 AA AA TT TT
C4_53426743 A/G 12 53426743 GG GG AA AA
C4_53426794 A/G 12 53426794 GG GG AA AA
C4_53426812 G/A 12 53426812 AA AA GG GG
C4_53426815 T/A 12 53426815 AA AA TT TT Table 30: SNP and indel markers on carinata chromosome C5.
C5_17545689 C/T 13 17545689 TT TT CC TT
C5_18337376 +/- 13 18337376 - ++ ++ ++
C5_25474382 G/A 13 25474382 GG GG AA GG
C5_26042650 +/- 13 26042650 - ++ ++ ++
C5_27102262 C/T 13 27102262 CC CC TT TT
C5_27782232 G/A 13 27782232 AA GG GG GG
C5_27782234 +/- 13 27782234 - ++ ++ ++
C5_28778609 +/- 13 28778609 - ++ ++ ++
C5_35449882 A/T 13 35449882 AA AA TT AA
C5_36675849 G/C 13 36675849 GG GG CC GG
C5_36713233 T/C 13 36713233 TT TT CC TT
C5_36789687 A/G 13 36789687 AA AA GG AA
C5_36929185 +/- 13 36929185 - ++ ++ -
C5_36970999 C/T 13 36970999 CC CC TT CC
C5_37097664 T/C 13 37097664 TT TT CC TT
C5_37251392 +/- 13 37251392 - ++ ++ ++
C5_38104699 T/G 13 38104699 GG GG TT GG
C5_38104777 T/C 13 38104777 CC CC TT CC
C5_38104808 T/C 13 38104808 CC CC TT CC
C5_38104843 C/T 13 38104843 TT TT CC TT
C5_38678731 G/A 13 38678731 AA AA GG GG
C5_38678765 G/A 13 38678765 AA AA GG GG
C5_38753994 C/T 13 38753994 TT TT CC TT
C5_40054532 T/A 13 40054532 TT TT AA TT
C5_40054533 T/C 13 40054533 TT TT CC TT
C5_40054577 G/A 13 40054577 GG GG AA GG
C5_40054593 G/A 13 40054593 GG GG AA GG
C5_40054756 G/C 13 40054756 GG GG CC GG
C5_40058118 C/G 13 40058118 CC CC GG CC
C5_40058127 C/T 13 40058127 CC CC TT CC
C5_40058166 A/G 13 40058166 AA AA GG AA
C5_40058188 T/A 13 40058188 TT TT AA TT
C5_40058191 G/A 13 40058191 GG GG AA GG
C5_40058224 C/T 13 40058224 CC CC TT CC
C5_40380584 A/C 13 40380584 AA AA CC AA
C5_41138635 +/- 13 41138635 - ++ - ++
C5_41390375 A/T 13 41390375 AA TT AA TT
C5_41518695 T/G 13 41518695 GG TT GG TT
C5_42950437 A/G 13 42950437 AA AA GG AA
C5_44114003 A/T 13 44114003 AA AA TT AA Table 31: SNP and indel markers on carinata chromosome C6.
C6_16848746 C/G 14 16848746 CC GG CC GG
C6_17323368 T/C 14 17323368 CC TT TT TT
C6_18030349 G/T 14 18030349 GG GG TT GG
C6_18151727 T/G 14 18151727 GG TT GG TT
C6_18151805 G/A 14 18151805 AA GG AA GG
C6_18882747 G/A 14 18882747 AA AA GG AA
C6_18882816 G/T 14 18882816 TT TT GG TT
C6_18882819 A/G 14 18882819 GG GG AA GG
C6_19044288 C/G 14 19044288 GG CC CC CC
C6_20876797 A/T 14 20876797 AA AA TT AA
C6_20876961 C/T 14 20876961 CC CC TT CC
C6_20971447 G/A 14 20971447 AA GG AA GG
C6_20971505 C/T 14 20971505 TT CC TT CC
C6_22640278 -/+ 14 22640278 ++ ++ - ++
C6_22640280 -/+ 14 22640280 ++ ++ - ++
C6_22640318 A/T 14 22640318 TT TT AA TT
C6_22656269 -/+ 14 22656269 ++ -- - -
C6_22721393 +/- 14 22721393 - -- ++ -
C6_22721425 G/T 14 22721425 TT TT GG TT
C6_22866300 -/+ 14 22866300 ++ ++ - ++
C6_22866325 C/G 14 22866325 GG GG CC GG
C6_23081200 T/C 14 23081200 CC CC TT CC
C6_23993706 A/G 14 23993706 GG AA GG AA
C6_23993718 A/T 14 23993718 TT AA TT AA
C6_24019940 T/C 14 24019940 CC TT CC TT
C6_24449411 C/T 14 24449411 CC CC TT CC
C6_24449418 T/C 14 24449418 TT TT CC TT
C6_24449473 C/A 14 24449473 CC CC AA CC
C6_24449477 C/T 14 24449477 CC CC TT CC
C6_25720166 G/C 14 25720166 CC GG GG GG
C6_25720171 C/T 14 25720171 TT CC CC CC
C6_25720366 T/C 14 25720366 CC TT TT TT
C6_26585077 C/T 14 26585077 CC CC TT CC
C6_26840602 A/C 14 26840602 AA AA CC AA
C6_26840631 C/G 14 26840631 CC CC GG CC
C6_27006788 A/G 14 27006788 AA AA GG AA
C6_27320829 C/A 14 27320829 CC CC AA CC
C6_27827378 C/T 14 27827378 CC CT CC CC
C6_28278252 G/A 14 28278252 AA AA GG AA
C6_29254507 C/T 14 29254507 CC CC TT CC
C6_29514323 T/G 14 29514323 TT TT GG TT C6_29520688 T/C 14 29520688 TT TT CC TT
C6_29520718 C/T 14 29520718 CC CC TT CC
C6_29532194 C/T 14 29532194 CC CC TT CC
C6_29555286 A/C 14 29555286 AA AA CC AA
C6_29555363 C/G 14 29555363 CC CC GG CC
C6_30033684 G/A 14 30033684 AA AA GG AA
C6_30033699 T/A 14 30033699 AA AA TT AA
C6_30427059 A/C 14 30427059 AA AA CC AA
C6_30427081 A/G 14 30427081 AA AA GG AA
C6_30430778 T/G 14 30430778 TT TT GG TT
C6_31139142 T/C 14 31139142 TT TT CC TT
C6_31139163 G/T 14 31139163 GG GG TT GG
C6_31139226 T/C 14 31139226 TT TT CC TT
C6_32122093 C/G 14 32122093 GG GG CC GG
C6_34943575 +/- 14 34943575 - ++ ++ -
C6_36597190 T/A 14 36597190 TT AA TT TT
C6_39032026 +/- 14 39032026 - ++ ++ ++
Table 32: SNP and indel markers on carinata chromosome C7.
C7_29185256 +/- 15 29185256 ++ ++
C7_30487954 +/- 15 30487954 ++ ++ -
C7_33396571 T/C 15 33396571 TT TT CC CC
C7_34452746 T/A 15 34452746 TT TT AA AA
C7_35269822 T/A 15 35269822 TT TT AA AA
C7_36185793 T/C 15 36185793 cc CC TT TT
C7_37344449 +/- 15 37344449 ++ ++ -
C7_39320758 A/G 15 39320758 GG GG AA AA
C7_39320815 T/C 15 39320815 CC CC TT TT
C7_39797620 A/G 15 39797620 GG AA GG AA
C7_40368011 G/A 15 40368011 GG GG AA AA
C7_40404145 C/T 15 40404145 CC CC TT TT
C7_40404156 G/T 15 40404156 GG GG TT TT
C7_40435622 C/G 15 40435622 GG GG CC CC
C7_40653772 C/T 15 40653772 CC CC TT TT
C7_40718159 G/A 15 40718159 AA AA GG GG
C7_41809998 A/G 15 41809998 GG GG AA AA
C7_41810007 C/T 15 41810007 TT TT CC CC
C7_41810010 C/T 15 41810010 TT TT CC CC
C7_41810151 C/T 15 41810151 TT TT CC CC
C7_41810157 T/A 15 41810157 AA AA TT TT
C7_41810174 A/G 15 41810174 GG GG AA AA
C7_41810211 T/C 15 41810211 CC CC TT TT
C7_42734039 A/G 15 42734039 GG GG AA GG
C7_42734095 G/A 15 42734095 AA AA GG AA
C7_43196058 C/G 15 43196058 CC CC GG CC
C7_43295854 +/- 15 43295854 ++ ++
C7_43304964 A/G 15 43304964 GG GG AA AA
C7_43345503 T/G 15 43345503 GG GG TT TT
C7_43352313 T/C 15 43352313 CC CC TT TT
C7_43451277 +/- 15 43451277 ++ ++ ++
C7_44017882 +/- 15 44017882 ++ ++
C7_44949816 T/A 15 44949816 TT TT TT AA
C7_44949822 A/G 15 44949822 AA AA AA GG
C7_44949828 A/G 15 44949828 AA AA AA GG
C7_45644517 G/T 15 45644517 TT GG GG GG
C7_45644539 C/T 15 45644539 TT CC CC CC
C7_45644544 C/T 15 45644544 CC CC TT TT
C7_45644559 C/G 15 45644559 CC CC GG GG
C7_45644565 A/T 15 45644565 AA AA TT TT
C7_45644567 A/C 15 45644567 AA AA CC CC C7_45644570 A/G 15 45644570 GG AA AA AA
C7_45644571 A/G 15 45644571 GG AA AA AA
C7_45644581 C/A 15 45644581 AA AA CC CC
C7_46217833 T/C 15 46217833 TT TT CC TT
C7_46217845 C/T 15 46217845 TT CC TT CC
Table 33: SNP and indel markers on carinata chromosome C8.
C8_32201323 G/T 16 32201323 GG TT GG GG
C8_32201350 G/C 16 32201350 GG CC GG GG
C8_32201353 C/T 16 32201353 CC TT CC CC
C8_32201374 T/C 16 32201374 TT CC TT TT
C8_32232879 T/A 16 32232879 AA TT TT TT
C8_32232931 G/T 16 32232931 TT GG GG GG
C8_32232937 +/- 16 32232937 - ++ ++ ++
C8_32232944 C/A 16 32232944 AA CC CC CC
C8_32315514 T/C 16 32315514 CC TT TT TT
C8_32315528 T/C 16 32315528 CC TT TT TT
C8_33640895 C/T 16 33640895 CT CC CC CC
C8_33640903 -/+ 16 33640903 -+ -- - -
C8_33729431 T/C 16 33729431 TT TT CC CT
C8_33729463 A/G 16 33729463 AA AA GG GG
C8_33729494 T/C 16 33729494 TT TT CC CC
C8_33729509 G/A 16 33729509 GG GG AA AA
C8_33729608 T/C 16 33729608 TT TT CC CC
C8_35634493 G/A 16 35634493 AG GG GG GG
C8_35805925 T/A 16 35805925 TT TT AA AA
C8_35805945 G/A 16 35805945 GG GG AA AA
C8_35805960 G/A 16 35805960 GG GG AA AA
C8_35809971 T/C 16 35809971 TT TT CC CC
C8_36353741 +/- 16 36353741 - ++ ++ ++
C8_36354350 C/A/T 16 36354350 CC CC CC AA
C8_37203182 G/A 16 37203182 GG AG GG GG
C8_37698686 T/C 16 37698686 CC TT TT CC
C8_37698950 -/+ 16 37698950 ++ -- - ++
C8_38414269 A/T 16 38414269 AA AA TT TT
C8_38414317 A/G 16 38414317 AA AA GG GG
C8_38414431 T/C 16 38414431 TT TT CC CC
C8_38414443 C/T 16 38414443 CC CC TT TT
C8_38414449 C/G 16 38414449 CC CC GG GG
C8_38419315 +/- 16 38419315 ++ ++ - -
C8_38440964 C/A 16 38440964 CC CC AA AA
C8_38441013 A/G 16 38441013 AA AA GG GG
C8_38442686 G/A 16 38442686 GG GG AA AA
C8_38637684 A/G 16 38637684 GG GG AA AA
C8_39593512 C/A 16 39593512 AA AC CC CC
C8_41291159 G/A 16 41291159 GG GG AA GG
C8_41646633 C/G 16 41646633 GG GG CC GG Table 34: SNP and indel markers on carinata chromosome C9.
C9_10496502 G/T 17 10496502 GG GG TT GG
C9_17146315 G/C 17 17146315 CC GC CC CC
C9_17720710 C/T 17 17720710 TT TT CC CC
C9_17720729 T/G 17 17720729 GG GG TT TT
C9_17720754 T/A 17 17720754 AA AA TT TT
C9_17770546 G/A 17 17770546 AA AA GG GG
C9_18613556 T/C 17 18613556 CC CC TT TT
C9_19289756 C/A 17 19289756 AA AA CC CC
C9_19289827 A/G 17 19289827 GG GG AA AA
C9_19289835 A/T 17 19289835 TT TT AA AA
C9_19289837 A/C 17 19289837 CC CC AA AA
C9_20058811 C/T 17 20058811 TT TT CC CC
C9_20058834 G/A 17 20058834 AA AA GG GG
C9_20141737 T/G 17 20141737 GG GG TT TT
C9_20141756 A/G 17 20141756 GG GG AA AA
C9_24205200 +/- 17 24205200 - ++ ++ ++
C9_30221517 +/- 17 30221517 - ++ ++ -
C9_31103055 +/- 17 31103055 - ++ ++ -
C9_42557630 A/G 17 42557630 GG GG AA AA
C9_42619426 C/A 17 42619426 AA AA CC CC
C9_42863241 C/T 17 42863241 TT TT CC CC
C9_42863293 C/T 17 42863293 TT TT CC CC
C9_42869989 +/- 17 42869989 - ++ ++
C9_43003823 A/G 17 43003823 GG GG AA AA
C9_43105259 C/T 17 43105259 TT TT CC CC
C9_43199256 G/C 17 43199256 CC CC GG GG
C9_43458273 A/G 17 43458273 GG GG AA AA
C9_43458352 G/T 17 43458352 GG GG TT TT
C9_43808934 A/G 17 43808934 AA AA AA GG
C9_43809004 G/C 17 43809004 GG GG GG CC
C9_43809015 A/G 17 43809015 AA AA AA GG
C9_43817827 G/T 17 43817827 GG GG GG TT
C9_43817859 T/C 17 43817859 TT TT TT CC
C9_43817984 T/C 17 43817984 TT TT TT CC
C9_45319859 C/T 17 45319859 TT TT CC CC
C9_45319868 T/C 17 45319868 CC CC TT TT
C9_49816416 G/A 17 49816416 GG GG GG AA
C9_49816530 G/A 17 49816530 GG GG GG AA
C9_49816536 +/- 17 49816536 ++ ++ ++ -
C9_50008950 +/- 17 50008950 ++ ++ - -
C9_50424646 A/G 17 50424646 GG AA GG AA C9_50424663 -/+ 17 50424663 ++ -- ++ -
C9_50424667 -/+ 17 50424667 ++ -- ++ -
C9_50424745 G/A 17 50424745 AA GG AA GG
C9_51300325 C/T 17 51300325 CC CC TT TT
C9_51832143 T/A 17 51832143 AA TT AA TT
C9_51832149 C/G 17 51832149 GG CC GG CC
C9_51832179 G/T 17 51832179 TT GG TT GG
C9_52000849 T/A 17 52000849 AA TT TT TT
C9_52000858 -/+ 17 52000858 ++ -- - -
C9_52000921 T/G 17 52000921 GG TT TT TT
C9_52105915 T/C 17 52105915 CC CC TT TT
C9_52252000 G/T 17 52252000 TT GG GG GG
C9_54035506 C/T 17 54035506 CT CC CC CC
C9_54365078 T/C 17 54365078 TT TT CC TT
C9_54366668 G/C 17 54366668 GG GG CC GG
[0046] In another embodiment, the invention provides a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0047] In another embodiment, the invention provides a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015, and wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
[0048] In another embodiment, the invention provides a seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0049] In another embodiment, the invention provides a cell of a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123015.
[0050] In another embodiment, the invention provides a cell of a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123015, and wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
[0051] In another embodiment, the invention provides a cell of a seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-312D, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123015.
[0052] In another embodiment, the invention provides a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
[0053] In another embodiment, the invention provides a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014, and wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
[0054] In another embodiment, the invention provides a seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014.
[0055] In another embodiment, the invention provides a cell of a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123014.
[0056] In another embodiment, the invention provides a cell of a plant, or part thereof, whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA- 123014, and wherein the plant part is an ovule, a leaf, pollen, a seed, an embryo a root, a root tip, a pod, a flower, a stalk, a cell, or a protoplast.
[0057] In another embodiment, the invention provides a cell of a seed whose genotype has greater than 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the single nucleotide polymorphisms and indel markers of Tables 19 to 35 in common with AGR044-3A22, wherein a representative sample of said seed has been deposited under ATCC Accession number PTA-123014. 6. DEPOSITS
[0058] Applicant has made a deposit of at least 2500 seeds of Brassica carinata Cultivar AGR044-312D with the American Type Culture Collection (ATCC), Manassas, VA 20110 USA, ATCC Deposit No. PTA- 123015. The seeds deposited with the ATCC on April 12, 2016 were taken from the deposit maintained by Agrisoma Biosciences Inc. since prior to the filing data of this application. This deposit of the Brassica carinata Cultivar AGR044-312D will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicant has satisfied all the requirements of 37 C.F.R. §§1.801- 1.809, including providing an indication of the viability of the sample. Applicant imposes no restrictions on the availability of the deposited material from the ATCC; however, Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant does not waive any infringement of his rights granted under this patent or under the Plant Variety Protection Act (7 USC 2321 et seq.).
[0059] Applicant has made a deposit of at least 2500 seeds of Brassica carinata Cultivar AGR044-3A22 with the American Type Culture Collection (ATCC), Manassas, VA 20110 USA, ATCC Deposit No. PTA- 123014. The seeds deposited with the ATCC on April 12, 2016 were taken from the deposit maintained by Agrisoma Biosciences Inc. since prior to the filing data of this application. This deposit of the Brassica carinata Cultivar AGR044-3A22 will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicant has satisfied all the requirements of 37 C.F.R. §§1.801- 1.809, including providing an indication of the viability of the sample. Applicant imposes no restrictions on the availability of the deposited material from the ATCC; however, Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant does not waive any infringement of his rights granted under this patent or under the Plant Variety Protection Act (7 USC 2321 et seq.).
[0060] The foregoing invention has been described in detail by way of illustration and example for purposes of exemplification. However, it will be apparent that changes and modifications such as single gene modifications and mutations, somatoclonal variants, variant individuals selected from populations of the plants of the instant cultivar, and the like, likewise are considered to be within the scope of the present invention. 7. REFERENCES
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