Title:
BRUCELLA SUIS STRAIN WITH BI-1 GENE DELETED, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/103733
Kind Code:
A1
Abstract:
Provided are a Brucella suis strain CGMCC NO.18741 with the BI-1 gene deleted, and a construction method therefor and a use thereof. The strain is obtained by means of replacing the BI-1 gene of Brucella suis with a kanamycin resistance gene. The strain with the gene deleted shows the characteristics of a slower growth rate, a reduced colony, an increased cell membrane permeability, an enhanced cell aggregation, etc., suggesting that the strain has potential as a live attenuated Brucella vaccine.
Inventors:
WANG AIHUA (CN)
ZHANG GUANGDONG (CN)
JIN YAPING (CN)
ZHOU DONG (CN)
ZHONG FANGLI (CN)
CHEN LEI (CN)
QIN PEIPEI (CN)
ZHI FEIJIE (CN)
LI JUNMEI (CN)
TIAN LULU (CN)
ZHANG GUANGDONG (CN)
JIN YAPING (CN)
ZHOU DONG (CN)
ZHONG FANGLI (CN)
CHEN LEI (CN)
QIN PEIPEI (CN)
ZHI FEIJIE (CN)
LI JUNMEI (CN)
TIAN LULU (CN)
Application Number:
PCT/CN2020/113414
Publication Date:
June 03, 2021
Filing Date:
September 04, 2020
Export Citation:
Assignee:
UNIV NORTHWEST A&F (CN)
International Classes:
C12N1/21; A61K39/10; A61P31/04; C12N15/74; G01N33/569; C12R1/01
Domestic Patent References:
| WO2007058267A1 | 2007-05-24 |
Foreign References:
| CN110699312A | 2020-01-17 | |||
| CN110484484A | 2019-11-22 | |||
| CN1516735A | 2004-07-28 | |||
| US20060134640A1 | 2006-06-22 |
Other References:
DATABASE Protein 17 September 2014 (2014-09-17), ANONYMOUS: "hypothetical protein BSS2_I0086 [Brucella suis bv. 1 str. S2]", XP055816548, retrieved from NCBI Database accession no. AHN45755
ZHANG, JUNMIN: "Screening and Identification of the Differential Genes of Genomic DNAs between Bovine Brucella Virulent and Vaccine Strain", ACADEMIC FORUM PROCEEDINGS OF VETERINARY BIOLOGICS AND VETERINARY MICROBIOLOGY 2008. PROCEEDINGS OF THE CHINESE SOCIETY OF ANIMAL HUSBANDRY AND VETERINARY MEDICINE, vol. 1, 1 November 2008 (2008-11-01), pages 253 - 257, XP009528545
SONGLIN XIE, YUFU WANG, WEI WEI, CHONGYANG LI, YI LIU, JINSONG QU, QIANGHONG MENG, YANG LIN, WEIXIAO YIN , YINONG YANG , CHAOXI L: "The Bax inhibitor UvBI-1, a negative regulator of mycelial growth and conidiation, mediates stress response and is critical for pathogenicity of the rice false smut fungus Ustilaginoidea virens", CURRENT GENETICS, vol. 65, no. 5, 11 April 2019 (2019-04-11), pages 1185 - 1197, XP036885192, ISSN: 0172-8083, DOI: 10.1007/s00294-019-00970-2
ZHANG, JUNMIN: "Screening and Identification of the Differential Genes of Genomic DNAs between Bovine Brucella Virulent and Vaccine Strain", ACADEMIC FORUM PROCEEDINGS OF VETERINARY BIOLOGICS AND VETERINARY MICROBIOLOGY 2008. PROCEEDINGS OF THE CHINESE SOCIETY OF ANIMAL HUSBANDRY AND VETERINARY MEDICINE, vol. 1, 1 November 2008 (2008-11-01), pages 253 - 257, XP009528545
SONGLIN XIE, YUFU WANG, WEI WEI, CHONGYANG LI, YI LIU, JINSONG QU, QIANGHONG MENG, YANG LIN, WEIXIAO YIN , YINONG YANG , CHAOXI L: "The Bax inhibitor UvBI-1, a negative regulator of mycelial growth and conidiation, mediates stress response and is critical for pathogenicity of the rice false smut fungus Ustilaginoidea virens", CURRENT GENETICS, vol. 65, no. 5, 11 April 2019 (2019-04-11), pages 1185 - 1197, XP036885192, ISSN: 0172-8083, DOI: 10.1007/s00294-019-00970-2
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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