BUFFER EXCHANGE OF BIOLOGICAL SAMPLES IN-LINE WITH SEPARATION AND MEASUREMENT OPERATIONS

Title:

BUFFER EXCHANGE OF BIOLOGICAL SAMPLES IN-LINE WITH SEPARATION AND MEASUREMENT OPERATIONS

Document Type and Number:

WIPO Patent Application WO/2023/154874

Kind Code:

A3

Abstract:

A method can include receiving a biological sample including the target cells, e.g., at an inlet of a microfluidic structure. This can involve flowing the target cells, e.g., suspended in a first buffer, at a first flow rate within a main channel of the microfluidic structure. At least one focusing flow can be applied to the biological sample, using a second buffer at a second flow rate, to help establish a boundary defining a central region or streamline. The central region can contain the target cells within the main channel and promote ion diffusion between the first and second buffers.

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Inventors:

SWAMI NATHAN (US)
HUANG XUHAI (US)
VARHUE WALTER (US)
CASTRO KARINA TORRES (US)

Application Number:

PCT/US2023/062399

Publication Date:

November 23, 2023

Filing Date:

February 10, 2023

Export Citation:

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Assignee:

UNIV VIRGINIA PATENT FOUNDATION (US)
SWAMI NATHAN (US)
HUANG XUHAI (US)
VARHUE WALTER (US)
CASTRO KARINA TORRES (US)

International Classes:

B01L3/00; B01L1/00; C12M1/00; G01N1/20

Domestic Patent References:

WO2017184854A1

2017-10-26

Foreign References:

US20210397127A1	2021-12-23
US20100304429A1	2010-12-02
US20190210019A1	2019-07-11

Other References:

HUANG XUHAI, TORRES‐CASTRO KARINA, VARHUE WALTER, RANE ADITYA, RASIN AHMED, SWAMI NATHAN S.: "On‐chip microfluidic buffer swap of biological samples in‐line with downstream dielectrophoresis", ELECTROPHORESIS, VERLAG CHEMIE, HOBOKEN, USA, vol. 43, no. 12, 1 June 2022 (2022-06-01), Hoboken, USA, pages 1275 - 1282, XP093114170, ISSN: 0173-0835, DOI: 10.1002/elps.202100304

Attorney, Agent or Firm:

PERDOK, Monique M. et al. (US)

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