This invention relates to C-linked pyrazole derivatives, processes for their preparation and pharmaceutical compositions containing them. According to the invention we provide a compound of general formula (I) :

or a physiologically acceptable salt, solvate (e.g. hydrate) or a metabolically labile ester thereof in which

R represents a hydrogen atom or a group selected from C-^galkyl or

C ₂ _galkenyl;

R^ represents a hydrogen atom or a group selected from C ₁ _galkyl,

C3 _ η cy c 1 oa Iky 1 , C 3 _ ₇ cy c 1 oa Iky 1C -^ _ 4 a Iky 1 , C3_galkenyl, f luoroC ₁ _galkyl, f luoroC .galkenyl, phenyl, -(CH ₂ ) _k COR ⁵ or

-(CH ₂ ) _k S0 ₂ R ⁵ ;

R ^w represents a hydrogen atom or a group selected from

C- _L _galkyl optionally substituted by a hydroxy or C-^.galkoxy group,

C ₂ _galkenyl, f luoroC*__ galkyl , -(CH ₂ ) _m R ⁶ , -(CH ₂ ) _n COR ⁷ or

-(CH ₂ )pNR ⁸ COR ⁹ ;

R ⁴ represents a group selected from -C0 ₂ H, -NHS0 ₂ CF3 or a C-linked tetrazolyl group;

R represents a group selected from C**__galkyl, C ₂ _galkenyl,

C- _] __galkoxy or the group -NR ¹⁰ R ^1:L ;

R represents a phenoxy or benzyloxy group;

₇ R represents a hydrogen atom or a group selected from hydroxy,

C ₁ _galkyl, C-^.galkoxy, phenyl, phenoxy or the group -NR °R ,*

R represents a hydrogen atom or a C^_galkyl group; q R represents a hydrogen atom or a group selected from C ₁ _galkyl,

C-L_galkoxy, phenyl, benzyl, phenoxy or the group -NR R ;

R — and R which may be the same or different each independently represent a hydrogen atom or a C-^^alk l group or -NR ¹⁰ R forms a saturated heterocyclic ring which has 5 or 6 ring members and may optionally contain in the ring one oxygen atom; k represents zero or an integer from 1 to 4, preferably zero, 1 or

2, especially zero or 1; m represents an integer from 1 to 4, preferably 1 or 2, especially

n represents zero or an integer from 1 to 4, preferably zero, 1 or

2, especially 0 or 1; and p represents an integer from 1 to 4, preferably 1 or 2.

Where the compound of general formula (I) is optically active, said formula (I) is intended to cover all enantio errs , diastereoisomers and mixtures thereof including racemates . Where a compound of the present invention contains one or two double bonds, these may exist in the cis or trans configuration. Furthermore where such geometric isomers exist _r formula CD is intended to cover mixtures thereo .

The invention also includes within its scope the solvates, especially the hydrates, of compounds of general formula (I) .

Within the above definition the term 'alkyl' or 'alkoxy' as a group or part of a group means that the group is straight or branched. The term 'alkenyl' as a group or part of a group means that the group is straight or branched and contains at least one carbon-carbon double bond. The term 'cycloalkyl' as a group or part of a group may be, for example, a cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl group.

The term ' f luoroC-^.galkyl' or ' f luoroC^.galkenyl' means an alkyl or alkenyl group in which one or more hydrogen atoms have been substituted by a luorine atom- for example, -CH C or -CH---CHCF .

Within the above definition when -NR ¹⁰ R represents a saturated heterocyclic ring, this contains 5 or 6 ring members, one of which may be an oxygen atom. Suitable heterocyclic groups are a pyrrolidino, piperidino or morpholino group.

A preferred class of compounds of general formula (I) is that wherein the group R ¹ is a C-^.^al yl (for example, ethyl, n-propyl or n-butyl), especially a C _5alkyl, or C ₃ _5alkenyl group.

Particularly preferred are those compounds wherein R ¹ is an n-butyl, n-propyl, but-1-enyl or prop-1-enyl group.

Another preferred class of compounds of general formula (I) is that wherein the group R ² is a f luoroC- _j __galkyl group or the group (CH ₂ ) _j -SC^R . Particularly preferred are those compounds wherein R* ^** represents a f luoroC ₁ _ alkyl group, especially -CH2CF3, or R- ^> represents the group -NR ¹ R (where R and R ¹ each represent a

A further preferred class of compound of general formula (I) is that wherein the group R ^ώ s a group selected from C- _| __galkyl, preferably C-^ςalkyl, especially ethyl, isopropyl or isobutyl;- C3_ ₇ cycloalkyl, preferably C3_t- ₎ cycloalkyl, especially cyclobutyl; C _ ₇ cycloalkylC _ alkyl, preferably C _5cycloalkylC ₁ _ ₄ alkyl, especially cyclopropylmethyl; or phenyl.

Another preferred class of compound of general formula (I) is that wherein the group R is adjacent to the group R ^J .

Yet another preferred class of compounds of general formula (I) is that wherein R** ⁵ is selected from a hydrogen atom or a group selected from C-^.galkyl, preferably C _] __ alkyl, optionally substituted by hydroxy or especially methoxy; or

-(CH )--R , especially wherein R° is a benzyloxy group; or

-(CH ) _n COR 7 , especially wherein R 7 represents hydrogen, hydroxy or

C ₁ _3alkoxy, especially methoxy, and m is 1 or 2 and n is zero, 1 or

2, especially zero or 1. In particular, R may represent a hydrogen atom or a group selected from methyl, ethyl, propyl, butyl, -CH ₂ OH,

-CHO, -CH ₂ OCH ₃ or -C0 ₂ H .

Another preferred class of compounds of general formula (I) is that wherein R^ is the group - (CH ₂ ) _NR ⁸ COR-', especially wherein R ⁸ represents hydrogen or a C- _j ^alkyl group and R ⁹ represents hydrogen or a C-L_ alkyl or C-^.galkoxy group.

Preferably, in the compounds of general formula (I) , R ⁴ may be the group -C0 ₂ H, or a C-linked tetrazolyl group.

Particularly preferred compounds are:

4' - [ [3-butyl-5- (methoxymethyl) -1- (2,2, 2-trif luoroethyl) -1H- pyrazol-4-yljmethyl] [1, 1' -biphenyl] -2-carboxylic acid;

4' - [ [5 -butyl -3- (methoxymethyl) -1- (2, 2, 2-trif luoroethyl) -1H- pyrazol-4-yl]methylj [1, 1' -biphenyl] -2-carboxylic acid;

5-[4'-[ [3-butyl-5- (methoxymethyl) -l-(2, 2, 2-trif luoroethyl) -1H- pyrazol-4-yl] methyl] [1,1' -biphenyl] -2-yl]-lH-tetrazole;

5- [4' [ [5-butyl-3- (methoxymethyl) -1- (2, 2, 2-trif luoroethyl) -1H- pyrazol-4-yl] methyl] [1, l'-biphenyl] -2-yl] -lH-tetrazole;

4' - [ [5-butyl-l- [ (dimethylamino ) sulphonyl] -3- (methoxymethyl) -1H- pyrazol-4-yl] methyl 1 [1, 1' -biphenyl] -2-carboxylic acid;

4' - [ [3-butyl-l- [ (dimethylamino) sulphonylj -5- (methoxymethyl) -1H- pyrazol-4-ylJmethyl] [1,1' -biphenyl] -2-carboxylic acid;

5- [4' - [ [3-butyl-l- [ (dimethylamino) sulphonyl] -5- (methoxymethyl) - lH-pyrazol-4-yllmethyl] [1, 1' -biphenyl] -2-yl] -lH-tetrazole;

5- [4' - [ [5-butyl-l— [ (dimethylamino) sulphonyl] -3- (methoxymethyl) - lH-pyrazol-4-yl] methyl] [1, l'-biphenyl] -2-yl] -lH-tetrazole; and the physiologically acceptable salts, solvates and metabolically labile esters thereof.

Further particularly preferred compounds of the present invention include:

3-butyl-l-ethyl-4- [ [2' - ( lH-tetrazol-5-yl) [1,1' -biphenyl] -4- yl]methyl]-lH-ρyrazole-5-methanol;

3-butyl-l- (1-methylethyl) -4- [ [2' - (lH-tetrazol-5-yl) [1, 1' - biphenyl] -4-yl]methyl] -lH-pyrazole-5-methanol;

3-butyl-l- (2-methylpropyl) -4- [ [2' - (lH-tetrazol-5-yl) [1, l'- biphenyl] -4-yl] methyl] -lH-pyrazole-5-methanol;

3-butyl-l- (2-cyclopropylmethyl) -4- [ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl] -4-yl]methyl] -lH-ρyrazole-5-methanol;

3-butyl-l-cyclobutyl-4- [ [2' - (lH-tetrazol-5-yl) [1, 1' -biphenyl] - 4-yl] methyl] -lH-pyrazole-5-methanol; l,3-dibutyl-4-[[2'- (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl] methyl] -lH-pyrazole-5-methanol; l-ethyl-3-propyl-4- [ [2' - (lH-tetrazol-5-yl) [1, l'-biphenyl] -4- yl] methyl] -lH-pyrazole-5-methanol;

1- (1-methylethyl) -3-propyl-4- [ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl] -4-yl] methyl] -lH-pyrazole-5-methanol;

3-butyl-l- (1-methylethyl) -4- [ [2' - (lH-tetrazol-5-yl) [1, 1' - biphenyl] -4-yl] methyl] -lH-pyrazole-5-carboxaldehyde;

3-butyl-l- (2-methylpropyl) -4-[ [2'- (lH-tetrazol-5-yl) [1, 1' - biphenyl] -4-yl]methyl] -lH-pyrazole-5-carboxaldehyde;

3-butyl-l- (2-cyclopropylmethyl) -4- [ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl] -4-yl]methyl] -lH-pyrazole-5-carboxaldehyde;

3-butyl-l-cyclobutyl-4-[ [2'- (lH-tetrazol-5-yl) [1, 1'-biphenyl]- 4-yl] ethyl]-lH-pyrazole-5-carboxaldehyde;

1, 3-dibutyl-4- [ [2'- (lH-tetrazol-5-yl) [1,1' -biphenyl] - - yl]methyl]-lH-pyrazole-5-carboxaldehyde;

1- (1-methylethyl) -3-propyl-4- [ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl]-4-yl]methyl]-lH-ρyrazole-5-carboxaldehyde;

3-butyl-l- (1-methylethyl) -4-[ [2'- (lH-tetrazol-5-yl) [1,1'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid;

3-butyl-l- (2-methylpropyl) -4-[ [2' - (lH-tetrazol-5-yl) [1, 1' - biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid;

3-butyl-l-cyclobutyl-4-[ [2'- (lH-tetrazol-5-yl) [1, 1'-biphenyl)- -yl]methyl]-lH-ρyrazole-5-carboxylic acid;

3-butyl-4-[ (2'-carboxyfl,l'-biphenyl]-4-yl)methyl]-1-ethyl-lH- pyrazole-5-carboxylic acid;

3-butyl-l-propyl-4-[ [2'- (lH-tetrazol-5-yl) [1, 1' -biphenyl]-4- yl]methyl]-lH-pyrazole-5-methanol; l-ethyl-3-propyl-4-[ [2'- (lH-tetrazol-5-yl) [1, 1'-biphenyl]-4- yl]methyl]-lH-pyrazole-5-carboxaldehyde; l-ethyl-3-propyl-4-[ [2'- (lH-tetrazol-5-yl) [1,1' -biphenyl]-4- yl]methyl]-lH-pyrazole-5-carboxylic acid;

1- (1-methylethyl) -3-propyl-4- [ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid;

1, 3-dibutyl-4- [ [2'- (lH-tetrazol-5-yl) [1,1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxylic acid;

3-butyl-4- [ (2' -carboxy [1, 1' -biphenyl] -4-yl) ethyl] -1- (1- methylethyl)-lH-pyrazole-5-carboxylic acid;

3-butyl-l-ethyl-4-[ [2 ' - (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxaldehyde;

3-butyl-l-propyl-4-[ [2'- (lH-tetrazol-5-yl) [1,1'-biphenyl]-4- yl]methyl]-lH-pyrazole-5-carboxaldehyde;

1- (2-methylpropyl) -3-propyl-4-[ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid;

3-butyl-l-propyl-4-[ [2 ' - (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxylic acid;

3-butyl-l-ethyl-4-[ \2 ^< - (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxylic acid;

3-butyl-l-(2-cyclopropylmethyl) -4- [ [2' - (lH-tetrazol-5-yl) [1,1'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carbo..ylic acid;

3-butyl-l-ethyl-4- [ [2' - [ [ (trif luoromethyl) sulphonyl] amino] [1, l'-biphenyl] -4-yl]methyl] -lH-pyrazole-5-carboxylic acid; l-ethyl-3-propyl-4-[ [2' - [ [ (trif luoromethyl) sulphonyl] amino] [1,1' -biphenyl] -4-yl] methyl] -lH-pyrazole-5-carboxylic acid;

5- [4' - [ [3-butyl-l-ethyl-5- (methoxymethyl) -lH-pyrazol-4- yl]methyl] [1, 1' -biphenyl] -2-yl] -lH-tetrazole; and the physiologically acceptable salts, solvates and metabolically labile esters thereof.

In accordance with the first aspect of the present invention, there is also provided a compound of the general formula (I) above or a physiologically acceptable salt, solvate or metabolically labile ester thereof wherein R ¹ represents a C-^.galkyl group ;

R represents a hydrogen atom or group selected from C-^_galkyl, C3_--cycloalkyl, C3_ ₇ cycloalkylC ₁ _ ₄ alkyl, f luoroC ₁ _galkyl, phenyl, -(CH ₂ ) ₎ -COR ⁵ or -(CH ₂ ) _k S0 ₂ R ⁵ ;

R represents a group selected from C ₁ _gal)yl substituted by a hydroxy or C-^galkoxy group, -(CH ₂ )mR ^{6 or} -(CH ₂ ) _n COR ⁷ ; R represents a group selected from -CO2H, -NHSO2CF3 or a C-linked tetrazolyl group; R ⁵ represents the group NR ^R ¹¹ ; R represents a benzyloxy group; R represent a hydrogen atom or a hydroxy group;

R ° and R each independently represent a hydrogen atom or a C-^al yl group; k represents zero or an integer from 1 to 4; m represents an integer from 1 to 4; and n represents zero or an integer from 1 to 4.

The physiologically acceptable acid addition salts of the compounds of formula (I) may be derived from inorganic or organic acids. Examples of such salts include hydrochlo rides , hydrobromides , sulphates, phosphates, benzoates, methanesulphonates or trif luoroacetates .

The compounds may also form salts with suitable bases . Examples of such salts are alkali metal (e.g. sodium or potassium) , alkaline earth metal (e.g. calcium or magnesium), ammonium and substituted ammonium (e.g. dimethy lammonium, t r iethy lammonium, 2- hydroxyethyldimethylammonium, piperazinium, N, -dimeth 1-

piperazinium, tetraalkylammonium, piperidinium, ethylenediammonium and choline) .

It will be appreciated that, for pharmaceutical use, the salts referred to above will be physiologically acceptable, but other salts may find use, for example, in the preparation of the compounds of formula (I) and the physiologically acceptable salts thereof.

It will be further appreciated that the compounds of general formula (I) may be chemically modified in the form of compounds which in vivo (for example, by enzymic attack) will provide the parent compounds of general formula (I) . Such prodrugs may be, for example, physiologically acceptable metabolically labile ester derivatives. These may be formed by esterif ication, for example of any of the carboxylic acid groups in the parent compound of general formula (I), with prior protection of any other reactive groups present in the molecule. ^' Examples of such esters include lower alkyl esters (e.g. methyl or ethyl esters), alkenyl esters (e.g. vinyl or alkyl esters), alkynyl esters (e.g. ethynyl or propynyl esters), alkoxyalkyl esters, (e.g. methoxymethyl or 2-methoxyethyl esters), alkylthioalkyl esters (e.g. methylthiomethyl esters) haloalkyl esters (e.g. 2-iodoethyl or 2, 2, 2 , -trichloromethyl esters), alkanoyloxyalkyl esters (e.g. acetoxymethyl, 1-acetoxyethyl or pivaloyloxymethyl esters), alkoxycarbonyloxyalkyl esters (e.g. 1- ethoxycarbonyloxyethyl or 1-methoxycarbonyloxyethyl esters), aroyloxyalkyl esters (e.g. benzoyloxymethyl or 1-benzoyloxyethyl esters), substituted or unsubstituted aralkyl esters (e.g. benzyl or 4-amidobenzyl esters) , substituted or unsubstituted aminoethyl esters (e.g aminoalkyl or 2-N, -dimethylaminoethyl esters) or hydroxyalkyl esters (e.g. 2-hydroxyethyl or 2, 3-dihydroxypropyl esters) .

In addition to the above ester derivatives the present invention includes within its scope compounds of general formula (I) in the form of other physiologically acceptable equivalents, i.e. physiologically acceptable compounds which, like the metabolically labile esters, are converted n vivo into the parent compounds of general formula (I) .

According to a second aspect of the invention we provide a compound of general formula (I) or a physiologically acceptable

salt, solvate or metabolically labile ester thereof for use in therapy.

In particular, the compounds of the invention may be used in the treatment or prophylaxis of hypertension. They are also potentially useful for the treatment of cognitive disorders such as dementia (e.g. Alzheimer's disease) and other diseases such as renal failure, hyperaldosteronism, cardiac insufficiency, congestive heart failure, post-myocardial infarction, cerebrovascular disorders, glaucoma and disorders of intracellular homeostasis.

According to a further aspect of the invention we provide a compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof for use in the treatment of the aforementioned diseases, especially hypertension.

According to another aspect of the invention we provide a compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof for the manufacture of a therapeutic agent for the treatment of the aforementioned diseases, especially hypertension.

According to a further aspect of the invention we provide a method of treating the aforementioned diseases, especially hypertension, which method comprises administering an effective amount to a patient in need of such treatment of a compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof -

It will be appreciated that the compounds of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof may advantageously be used in conjunction with one or more other therapeutic agents, such as for example diuretics and/or different antihypertensive agents such as β-blockers, calcium channel blockers or ACE inhibitors . It is to be understood that such combination therapy constitutes a further aspect of the present invention .

It will be further appreciated that reference herein to treatment extends to prophylaxis as well as to the treatment and relief of established symptoms.

While it is possible that a compound of general formula (I) may be administered as the raw chemical it is preferable to present the active ingredient as a pharmaceutical formulation.

The compounds of general formula (I) and their physiologicall acceptable salts, solvates and metabolically labile esters may b formulated for administration in any convenient way, and th invention also includes within its scope pharmaceutical compositions comprising at least one compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof adapted for use in human or veterinary medicine. Such compositions may be presented for use in conventional manner in admixture with one or more physiologically acceptable carriers or excipients. The carrier (s) must be 'acceptable' in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

Thus, the compounds according to the invention may be formulated for oral, buccal, parenteral or rectal administration or in a form suitable for administration by inhalation or insufflation. Oral administration is preferred.

Tablets and capsules for oral administration may contain conventional excipients such as binding agents, for example mucilage of starch or polyvinylpyrrolidone; fillers, for example, lactose, microcrystalline cellulose or maize-starch; lubricants, for example, magnesium stearate or stearic acid; disintegrants, for example, potato starch, croscarmellose sodium or sodium starch glycollate; or wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known in the art. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example, sorbitol syrup, methyl cellulose, glucose /sugar syrup or carboxymethyl cellulose; emulsifying agents, for example, sorbitan mono-oleate; non-aqueous vehicles (which may include edible oils), for example, propylene glycol or ethyl alcohol; and preservatives, for example, methyl or propyl p_-hydroxybenzoates or sorbic acid. The compounds or their salts or esters may also be formulated as suppositories, e.g. containing conventional suppository bases such as cocoa butter or other glycerides. For buccal administration the

composition may take the form of tablets or lozenges formulated i conventional manner.

It will be appreciated that both tablets and capsules may be manufactured in the form of sustained release formulations, such that they provide a controlled continuous release of the compounds according to the invention over a period of hours.

The compounds of general formula (I) and their physiologically acceptable salts, solvates and metabolically labile esters may be formulated for parenteral administration by bolus injection or continuous infusion and may be presented in unit dose form in ampoules, or in multi-dose containers with an added preservative. The compositions may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising and/or dispersing agents. Alternatively the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. sterile, pyrogen-free water, before use.

For administration by inhalation the compounds according to the invention are conveniently delivered in the form of an aerosol spray presentation from pressurised packs or a nebuliser, with the use of a suitable propellant, e.g. dichlo rodi f luo rome hane , trichlorofluorome thane, dichlorotetrafluoroethane or other suitable gas. In the case of a pressurised aerosol the dosage unit may be determined by providing a valve to deliver a metered amount.

Alternatively, for administration by inhalation or insufflation, the compounds according to the invention may take the form of a dry powder composition, for example a powder mix of the compound and a suitable powder base such as lactose or starch. The powder composition may be presented in unit dosage form in, for example, capsules or cartridges of e.g. gelatin, or blister packs from which the powder may be administered with the aid of an inhaler or insufflator.

The pharmaceutical formulations according to the invention may also contain other active ingredients such as antimicrobial agents, or preservatives.

It will be appreciated that the amount of a compound of general formula (I) required for use in treatment will vary not only with the particular compound selected but also with the route of

administration, the nature of the condition being treated and the age and condition of the patient and will ultimately be at the discretion of the attendant physician or veterinarian. In general, however, when the compositions comprise dosage units, each unit will preferably contain 0. Img to 500mg, advantageously where the compounds are to be administered orally Img to 400mg of the active compound. The daily dosage as employed for adult human treatment will preferably range from O.lmg to 2g, most preferably from Img to lg which may be administered in 1 to 4 daily doses.

The compounds of the invention may be prepared by a number of processes as described below wherein the various groups are as defined for general formula (I) unless otherwise specified.

It will be appreciated by a person skilled in the art that where necessary, reactive or labile groups in the following processes may be protected in a conventional manner using, for example, one of the groups described in process (C) hereinafter.

Thus, according to a further aspect of the present invention we provide a process (A) for preparing the compounds of general formula (I) which comprises treating a compound of general formula (II)

(wherein R , R and R are as defined in general formula (I) ) with a hydrazine of formula (III)

R ² NHNH ₂ (III)

9

(wherein R is as defined in general formula (I) ) followed by the remova l of any protect ing groups where present , as described hereinafter .

The reaction is preferably ef fected in a solvent such as an aqueous alcohol e . g . ethanol , an ether e . g tet rahydrof uran or dioxan, a substituted amide e . g dimethyl formamide, acetonitrile or water at a temperature in the range of 0°C to reflux and preferably at room temperature .

The intermediate diketones of formula (II) are novel compound and form a further aspect of the invention.

In another general process (B) a compound of general formul (I) may be obtained by interconversion of another compound o general formula (I) . Thus for example, when R^ represents hydrogen atom, such a compound may be converted into a compound o general formula (I) wherein R represents a group -(CH ₂ ) C0 o -(CH ₂ ) _k S0 ₂ R ⁵ by reaction with -(CH ₂ ) _k COR ⁵ or L- (CH ₂ ) _k S0 R ⁵ , respectively (wherein represents a leaving group, for example, a halogen atom such as a chlorine, bromine or iodine, or a hydrocarbylsulphonyloxy group such as methanesulphonyloxy, or p- toluenesulphonyloxy) . The reaction is conveniently effected in a suitable solvent such as a substituted amide e.g dimethylformamide or an ether e.g tetrahydrofuran in the presence of a base such as sodium hydride or sodium amide, at a temperature in the range O^C to reflux, and preferably at room temperature.

In an alternative example of process (B) a compound of general formula (I) wherein R^ is a hydrogen atom may be converted into a compound of general formula (I) wherein ^ represents a C-^_galkyl, C3_ ₇ cycloaklyl or C _ ₇ cycloalkylC _4alkyl group, or a group -(CH ₂ ) _k C0R ⁵ or -(CH ₂ ) _k S0 ₂ R ⁵ where k is 1 to 4, by reaction with a corresponding alkylating agent, for example, an alkylhalide such as an alkyliodide. The reaction is conveniently effected in a suitable solvent such as a substituted amide e.g. dimethylformamide or an ether e.g. tetrahydrofuran in the presence of a base such as potassium carbonate or sodium hydride, at a temperature in the range of 0 C to reflux, and preferably at room temperature.

It will also be appreciated that other substituents in a compound of general formula (I) may be modified by techniques well known in the art to produce alternative compounds of general formula (I) -

In another general process (C) a compound of general formula (I) may be obtained by deprotection of a protected intermediate of general formula (la)

(wherein R , R , R and R are as defined in general formula (I except that at least one reactive group is blocked by a protectin group) . The protecting groups may be* any conventional protectin groups, for example as described in "Protective Groups in Organi Synthesis" by Theodora Greene (John Wiley and Sons Inc., 1981) Examples of carboxyl protecting groups include C-^.galkyl such a methyl or t-butyl, or C ₇ _2øaralkyl such as benzyl.

When R is a tetrazolyl group, this may be protected with, fo example, the trityl group -C (phenyl) , or a p_-nitrobenzyl or 1 ethoxyethyl group.

Deprotection to yield the compound of general formula (I) ma be effected using conventional techniques. Thus, for example aralkyl groups may be cleaved by hydrogenolysis in a suitabl organic solvent such as an alcohol, e.g. ethanol, in the presence o a noble metal catalyst such as palladium or an oxide thereof on support such as charcoal, and conveniently at room temperature an pressure. Carboxyl protecting groups such as alkyl groups may b cleaved by hydrolysis using a base such as an alkali metal hydroxid (e.g. sodium hydroxide or potassium hydroxide) in a suitabl solvent (e.g. an aqueous alcohol such as methanol or ethanol) a any suitable temperature up to reflux. Deprotection of th tetrazolyl group when protected with a trityl group may be effecte by acid hydrolysis using trifluoroacetic acid, a sulphonic acid suc as dl-10-camphor sulphonic acid, or a mineral acid such a hydrochloric acid in a suitable solvent such as methanol, ethanol, tetrahydrofuran or mixtures thereof conveniently at room temperatur to reflux. Alternatively, when possible, deprotection of th tetrazolyl group can be effected by catalytic hydrogenation a previously described.

In another general process (D) a compound of general formul (I) in which the substituent R represents a C-linked tetrazoly group, may also be prepared from a compound of general formula (IV)

(wherein, R 1 , 2 and R ^ are as defined in general formula (I) ) b reaction with a suitable azide such as sodium azide, ammonium azid

(preferably prepared in situ from sodium azide and ammoniu chloride) , trialkyl- (e .g.triethyl) ammonium azide (preferabl prepared in situ from sodium azide and a trialkylamine (e.g. trie thy lamine) ) , a trialkylsilylazide (e.g. trimethylsily lazide) o an alkyl azide e.g. tributyl tin azide. The reaction is conveniently effected in a solvent such as xylene, an ether, fo example, dimethoxyethane or tetrahydrofuran, or a substituted amide, for example, dimethylformamide, at an elevated temperature, such as the reflux temperature of the solvent, for between 1 and 10 days.

Where the azide is tributyl tin azide the reaction may conveniently be effected in the absence of a solvent at a temperature between room temperature and 180°C. Such a reaction leaves the tetrazolyl group protected with a tributyl tin group, which can readily be removed using aqueous base or acid. Where aqueous base is used to effect this deprotection, the compound may be treated with an aqueous acid to liberate the tetrazole.

Compounds of general formula (IV) may be prepared by processes analogous to those described herein commencing from a compound of formula (XV) _

The Intermediate compounds of general formula (IV) are novel compounds and form a further aspect of the present invention.

In another general process (E) a compound of general formula (I) in which the substituent R represents -NHS0 ₂ CF3, ^{mav be} prepared from a compound of general formula (V)

(V)

(wherein R , R ^*1 and R are as defined in general formula (I)) by reaction with t r i f lu or ome t h ane s u lph on i c anhydride or trif luoromethylsulphonyl chloride, in a suitable solvent such as a halogenated hydrocarbon, e.g. dichloromethane or chlorform.

Compounds of general formula (V) may be prepared by processes analogous to those described herein commencing from a compound of formula (XVI) or a nitro precursor thereof.

Alternatively, compounds of general formula (V) may be prepared by a Curtius rearrangement of a compound of formula (I) wherein R is -C0 ₂ H (provided that this is the only carboxyl group in the molecule) using, for example, diphenylphosphorylazide in the presence of a base such as triethylamine and in a solvent such as an alcohol (e.g. tert-butanol) to form a carbamate followed by deprotection of the amine in a conventional manner, for example by acid hydrolysis using hydrochloric acid in a solvent such as ethanol.

The intermediate compounds of general formula (V) and their acid addition salts are novel compounds and form a further aspect of the present invention.

In another general process (F) a compound of general formula (I) may be prepared by treating a compound of formula (VI) with a compound of formula (VII)

(VI) (VII)

1 *) *ι 3

(where one of R and R represents a halogen atom, for example, bromine or iodine, and the other represents the group -B(OH) ₂ or an ester thereof, and R , R , R ³ and R are as defined in general formula (I) ) .

The reaction may be effected in the presence of a transition metal catalyst such as tetrakis (triphenylphosphine) palladium (0), in a suitable solvent such as an ether (e.g. 1, 2-dimethoxyethane or

tetrahydro uran) or an aromatic hydrocarbon (e.g. benzene). The reaction is preferably carried out in the presence of a base such as an alkali or alkaline earth metal carbonate (e.g. sodium carbonate) at a suitable temperature up to reflux.

The intermediate compounds of formula (VI) are novel compounds and form a further aspect of the present invention.

In another general process (G) a compound of general formula

(I) wherein R ^J 3 represents the group -(Cf-2) _n COR7 where n is zero and

R ⁷ is C-L_ al o y, may be prepared by reacting a biphenyl compound of formula (VIII)

(wherein R is as defined in general formula (I) and L is a leaving group as defined above) with a compound of formula (IX)

(wherein R ¹ and R ² are as defined in general formula (I) and R ^a is a C^— ≥lkoxy group) .

The reaction is conveniently effected at a temperature between -100°C and room temperature in a suitable solvent such as an ether, for example, tetrahydrofuran, dimethoxyethane or diethyl ether.

In the processes (A) , (B) , (C) , (D) , (E) , (F) and (G) described above, the compounds of general formula (I) may be obtained in the form of a salt, conveniently in the form of a physiologically acceptable salt. Where desired, such salts may be converted into the corresponding free acids or free bases using conventional methods.

Physiologically acceptable salts of the compounds of general formula (I) may be prepared by reacting a compound of general formula (I) with an appropriate acid or base in the presence of a suitable solvent such as acetonitrile, acetone, chloroform, ethyl acetate or an alcohol, e.g. methanol, ethanol or isopropanol.

Physiologically acceptable salts may also be prepared from other salts, including other physiologically acceptable salts, of the compounds of general formula (I), using conventional methods.

The intermediate compounds of general formula (II) may be prepared from a compound of formula (X)

-i 3 ,

(wherein R and R are as defined in general formula (I)) by condensation with a compound of formula (XI)

(wherein R ^* is defined in general formula (I) and L is a leaving group, for example a halogen atom such as chlorine, bromine or iodine, or a hy dr o c a rby 1 s u lph o ny 1 oxy group such as methanesulphonyloxy, or p_-toluenesulphonyloxy) . The reaction is preferably effected under basic conditions, for example, in the presence of sodium hydride, potassium carbonate or sodium methoxide. The reaction is conveniently effected in a solvent such as acetonitrile or an ether e.g. tetrahydrofuran or dioxan, a ketone e.g. butanone or acetone, or a substituted amide e .g. dimethylf ormamide, at a temperature between 0 C and the reflux temperature of the solvent .

Compounds of formula (X) may be prepared by reaction of a compound of formula (XII)

R ¹ COCH ₃ (XII)

With a compound of formula (XIII)

R ³ C0 ₂ CH ₃ (XIII)

preferably in the presence of a base such as sodium amide, sodium hydride or tetra-n-butyl ammonium fluoride. The reaction is conveniently effected in a solvent such as an ether e.g.

tetrahydro uran or dioxan, or a halogenated hydrocarbon e.g. dichloromethane at a temperature between 0°C and the reflux temperature of the solvent.

Compounds of formula (VIII) and (XI) may be prepared from a compound of formula (XIV)

using any suitable reagent well known in the art for converting the methyl group in formula (XIV) into the group -CH ₂ L (wherein is as defined above) . Thus or example, when L is a halogen atom, a compound of formula (XIV) can be converted into a compound of formula (XI) using N-chloro amides , tert-butyl hypochlorite or ΪJ- bromosuccinimide . Halogenation may be catalysed by light, thus the reaction mixture can be illuminated with a suitable artificial light source, and preferably in the presence of a free radical initiator such as azobisisobutyronitrile (AIBN) or dibenzoyl peroxide . The reaction may be conveniently effected in a so lvent such as a halogenated hydrocarbon, e.g. carbon tetrachloride at an elevated temperature such as the reflux temperature of the solvent .

Compounds of formula (XIV) in which R represents a C-linked tetrazolyl group may be prepared from a compound of formula (XV)

using the reagents and conditions described in process (D) .

Compounds of formula (XIV) in which R represents the group -NHSO2CH3 may be prepared from a compound of formula (XVI)

using the reagents and conditions described in process (E) .

Compounds of formula (XIV) in which R represents -COOH or - KS0 ₂ CF3, and compounds of formulae (XV) and (XVI) , may be prepared by reaction of a compound of formula (XVII)

(wherein Z represents a bromine or iodine atom or the group -OS0 ₂ CF ₃ , and R ^4a represents either -COOH, -NHS0 ₂ CF3, a nitrile or amino group or a group convertible thereto by standard methodology) with a corresponding 4-methylbenzeneboronic acid derivative in the presence of a palladium ( 0 ) compound such as tetrakis (triphenylphosphine) palladium (0) in a solvent such as dimethoxyethane in the presence of a base such as sodium carbonate or thallium hydroxide. The reaction is conveniently effected at an elevated temperature, such as the reflux temperature of the solvent. Compounds of formulae (VI) or (VII) where R or R ¹³ represents -B(OH) ₂ may be prepared from the corresponding halides by lithiation at low temperature followed by reaction with a suitable boronic acid ester (e.g. triisopropylborate) and subsequent hydrolysis with water or an acid (e.g. hydrochloric acid) .

Alternatively, compounds of formula (VI) wherein R ¹² represents Hal may be prepared by the reaction of a compound of formula (XVIII)

(xv _jjj )

with a compound of formula (III) under the reaction conditions of general process (A) .

Compounds of formula (XVIII) may be prepared by reaction of a compound of formula (XIX)

(wherein Hal is a bromine or iodine atom and L is a leaving group) with a compound of formula (X) under basic conditions, for example, in the presence of sodium hydride , potassium carbonate or sodium methoxide . The reaction is conveniently effected in a solvent such as acetonitrile or an ether e . g . tetrahydrofuran , a ketone , e . g .

acetone or a substituted amine e.g. dimethylf ormamide, at a temperature between 0°C and the reflux temperature of the solvent.

Compounds of formula (IX) may be prepared by lithiation of a compound of formula (XX)

The reaction may be effected using an alkyllithium compound, for example, tert -butyl lithium at a temperature between -100°C and room temperature, in a suitable solvent such as an ether, for example, tetrahydrofuran, dimethoxyethane or diethyl ether.

Compounds of formula (XX) may be prepared by halogenation of a compound of formula (XXI)

using standard methodology described herein above.

Compounds of formula (XXI) may be prepared by the reaction of a compound of formula (X) wherein R -. represents -(CH ₂ ) _n COR 7 (where n

₇ is zero and is C-^galkoxy) with a compound of formula (III) using the method of general process (A) .

Intermediates of formulae (III) , (VII) , (VIII) , (XII) , (XIII) , (XVI) and (XVII) are either known compounds or may be prepared by methods analogous to those described herein or used for the preparation of the known compounds .

The following examples illustrate the invention. Temperatures are in °C. "Dried" refers to drying using magnesium sulphate. Thin layer chromatography was carried out on silca, using one of the following solvent systems: A - e t he r : exa n , B - dichloromethane rethanol: ammonia, C - etherrpetroleu ether (40-60°), D - ethyl acetate rhexane, E - ethyl acetate : ethanol , F - ether:acetic acid, G - ether : hexane : acetic acid, or H - ethyl

acetate: acetic acid. The following abbreviations are used: DFC - dry flash chromatography on silica gel (Merck 9385); THF- tetrahydrofuran; DME - dimethoxyethane ; AIBN azobisisobutyronitrile ; DMF - N, N-dimethylf ormamide ; TFA - trifluoroacetic acid.

Intermediate 1 l-Methoxy-2, 4-octandione

2-Hexanone (96.8g) was added dropwise over 15min under nitrogen to a stirred suspension of sodium amide (37.7g) in dry ether (1 litre) at room temperature. The mixture was stirred for 12min, a solution of methyl methoxyacetate (50.3g) in dry ether (150ml) was added dropwise over 20min, and the mixture was heated under reflux for 2h. The mixture was cooled, poured into ice and 2N hydrochloric acid (600ml) and the aqueous layer extracted with ether (4x 300ml) . The organic layers were washed with 8% sodium bicarbonate solution and brine, dried and evaporated to give an orange liquid (102g) . Purification by DFC, eluting with System A (5:95) gave a yellow liquid (36g) . T.l.c. (System A 5:95) Rf 0.1.

Intermediate 2

1, 1-Dimethylethyl 4'-bromomethylbiphenyl-3-carboxylate

(a) 1, 1-Dimethylethyl 2-bromobenzoate

A solution of c_-bromobenzoic acid (lg), N, N' -dicyclohexyl carbodiimide (1.129g), t-butanol (405mg) and 4-dimethylaminopyridine (61mg) in dry ether (20ml) was stirred at room temperature for 18h. The resulting precipitate was filtered off and the filtrate evaporated to give a colourless solid which was triturated under petroleum ether (40-60°) (20ml) and filtered. The filtrate was evaporated to give a pale yellow oil (0.994g) . b.p. 80-84°/0.35 mmHg.

(b) 1, 1-Dimethylethyl 4' -methylbiphenyl-2-carbo>:ylate

A mixture of Intermediate 2a (2.0g) 4-methylbenzeneboronic acid (1.17g), tetrakis (triphenylphosphine) palladium (0) (269mg) and sodium carbonate solution (1M; 21ml) in DME (80ml) was heated under reflux for 18h. The solvent was evaporated and the residue

partitioned between ether (3x 50ml) and sodium carbonate (2N; 100ml) and the combined organic extracts were washed with brine (2x 100ml) and dried. The solvent was evaporated to give a pale yellow oil which was purified by short-path column chromatography on silica gel (Merck 7729; 40g) . Elution with System C (1:40) gave a colourless, mobile oil (1.88g). T.l.c (System C 1:40) Rf 0.3.

(c) 1, 1-Dimethylethyl 4'-bromomethylbiphenyl-2-carboxylate A mixture of Intermediate 2b (1.825g), N-bromosuccinimide (1.21g) and dibenzoyl peroxide (130mg) in carbon tetrachloride (30ml) was heated under reflux for 18h. The solvent was evaporated in vacuo and the residue partitioned between ether (3x 50ml) and sodium bisulphite solution (50ml) . The combined organic extracts were dried and the solvent evaporated to give a yellow oil which was purified by shor -path column chromatogrpahy on silica gel (Merck 7729; 60g) . Elution with System C (1:50) gave a colourless crystalline solid (1.15g) . T.l.c (System C 1:50) Rf 0.25.

Intermediate 3

2- (4-Bromomethyl (phenyl) ) -2' - (triphenylmethyl) tetrazol-1-yl benzene

(a) 2- (4-Methylphenyl) benzonitrile

To a solution of o_~ bromobenzonitrile (40g) and 4 - methylbenzeneboronic acid (33g) in DME (2 litres) was added tetrakis

(triphenylphosphine) palladium (0) (7.58g) and then sodium carbonate

(1M, 592ml) . The mixture was stirred vigorously and heated at reflux for 18 hours. The solvent was removed in vacuo and the dark residue partitioned between ether (800ml) and sodium carbonate (1M, 800ml) ; the aqueous phase was extracted with ether (3x 400ml) . The combined organic phases were dried and concentrated in_ vacuo to afford an orange oil which was crystallised from System C (1:3) using activated charcoal as a decolourising agent to afford a white solid (33. Og) . T.l.c. System A (1:9) Rf 0.30

(b) 2- (4-Methylphenyl) -IH-tetrazol-l-yl benzene

A mixture of Intermediate 3a (7g) and tributyl tin azide _, (24g) was heated at 160°C. Further amounts of Intermediate 3a were added after 2h (4g) and a further 1.5h (2g) and heating then continued for another 1.5h. The cooled reaction mixture diluted with ether (200ml) and extracted with aqueous sodium hydroxide solution (2M;50ml). The aqueous mixture was poured into cold concentrated HCl (50ml) and the white solid filtered and air-dried. This solid was crystallised from toluene (200ml) to give the title compound as cream microcrystals (10.2g) . T.l.c. ether Rf. 040

(c) 2- (4-Methylphenyl) -2' - (triphenylmethyl) tetrazol-1-yl benzene

To a suspension of Intermediate 3b (12.2g) in dry dichloromethane (100ml) was added triethylamine (12.8ml). Trityl chloride (14.6g) was added followed by N, N-dimethylaminopyridine (251mg) and the resultant solution was stirred at room temperature for 16h. The reaction mixture was partitioned between water (200ml) and dichloromethane (200ml) . The separated organic phase was washed with water (200ml), dried and concentrated in vacuo to give the title compound as a cream-coloured solid (21.2g) . T.l.c. System A (1:1) Rf 0.55

(d) 2- (4-Bromomethyl (phenyl) ) -2' - (triphenylmethyl) tetrazol-1-yl benzene

To a solution of Intermediate 3c (21. lg) in carbon tetrachloride (600ml) was added N-bromosuccinimide (8.3g), and the mixture heated almost to reflux. Dibenzoyl peroxide (854mg) was added and the mixture heated at reflux for 3.5h. Further dibenzoyl peroxide (800mg) was added and the reaction mixture heated at reflux for a further 60h. Further N-bromosuccinimide (4.2g) and dibenzoyl peroxide (854mg) were added and the mixture heated at reflux in the presence of a 200W lamp for 3h. The cooled mixture was filtered and the filtrate washed with water (2x150ml) , dried and concentrated in vacuo to afford the title compound as a cream glassy solid (23g) . T.l.c (ethyl acetate :petroleum ether 1:1) Rf 0.90.

Intermediate 4

1, 1-Dimethylethyl 4 '- [2- (methoxyacetyl) -3-oxoheptanyl] [1, l'- biphenyl 1 -2-carboxylate

Intermediate 1 (13.4g) was heated under reflux with Intermediate 2 (30. Og) and anhydrous potassium carbonate (10.8g) in acetone (390ml) with stirring under nitrogen for 6h. The solvent was evaporated and the residue partitioned between water (300ml) and ether (3x 200ml) ; the organic extracts were washed with brine, dried and evaporated to give an orange oil .39.5g) . Purification by DFC eluting with System A (1:4) gave the title compound as a pale yellow oil (25g) . T.l.c. System A (1:3) Rf 0.1.

Intermediate 5 l-Methoxy-3-[ [2' -[2- (triphenylmethyl) -2H-tetrazol-5-yl] [1,1'- biphenyl] -4-yl]methyl] -2, -octandione

Sodium hydride (80% dispersion in oil, 200mg) was added to a stirred solution of Intermediate 1 (0.96g) in dry THF (20ml) at room temperature under nitrogen, and stirring was continued at room temperature for 40min. A solution of Intermediate 3 (2.08g) in THF

(5ml) was added, and the mixture was heated under reflux for 22h.

The mixture was poured into water (50ml) and extracted with ether

(3x 40ml) ; the organic layers were washed with brine, dried and evaporated to give a dark yellow oil (2.38g) . Purification by DFC eluting with System A (20:80 to 50:50) gave the title compound as pale yellow oil (0.8g).

T.l.c. (System A 40:60) Rf 0.2.

Intermediate 6

1,1-Dimethylethyl 4'-[ [3-buty 1-5- (methoxymethyl) -lH-pyrazol-4-yl] methyl] [1, 1' -biphenyl] -2-carboxylate

Intermediate 4 (0.5g) was stirred at room temperature with hydrazine hydrate (85%, 0.086ml) in absolute ethanol (15ml) under nitrogen for

16h. The solvent was evaporated to give a colourless oil (375mg) .

Purification by DFC eluting with System B (98:2:0.2) gave a colourless oil (325mg) .

T.l.c. System C (50:50), Rf 0.2.

Intermediate 7

(a) 1, 1-Dimethylethyl 4' - [ [5-butyl-3- (methoxymethyl) -1-phenyl-lH- pyrazol-4-yl]methyl] f1, l'-biphenyl]-2-carboxylate; and

(b) 1, 1-Dimethylethyl 4' - [ [3-butyl-5- (methoxymethyl) -1-phenyl-lH- pyrazol-4-yl]methyl] [1,1'-biphenyl]-2-carboxylate

Intermediate 4 (l.Og) was stirred with phenylhydrazine (0.224ml) in ethanol (absolute, 20ml) at room temperature under nitrogen for 16h and heated under reflux for 24h. The solvent was evaporated to give an orange oil (1.22g) . Purification by DFC eluting with System A (10:90 - 30:70) gave:

(a) Intermediate 7a as a pale yellow oil (229mg), T.l.c. (System C 50:50) Rf 0.4, and

(b) Intermediate 7b as a pale yellow oil (256mg) , T.l.c. (System C 50:50) Rf 0.75.

Intermediate 8

(a) 1, 1-Dimethylethyl 4' -[ [3-butyl-5- (methoxymethyl) -1- (2,2,2- trifluoroethyl) -lH-pyrazol-4-yl]methyl] [1, l'-biphenyl] -2- carboxylate; and

(b) 1, 1-Dimethylethyl 4'-[ [5-butyl-3- (methoxymethyl) -1- (2,2,2- trifluoroethyl) -lH-pyrazol-4-yl]methyl] [1,1'-biphenyl]-2-carboxylate Intermediate 4 (0.5g) was stirred at room temperature under nitrogen with 2, 2, 2-trifluoroethylhydrazine (70% in water, 0.204ml) in ethanol (10ml) for 24h. The solvent was evaporated and the residue

(0.7g) purified by DFC eluting with System A (10:90 -. 50:50) to give:

(a) Intermediate 8a as a pale yellow oil (180mg) , T.l.c (System A 50:50) Rf 0.55; and

(b) Intermediate 8b as a colourless oil (239mg), T.l.c. (System A 50:50) Rf 0.25.

Intermediate 9

5— T4 ^f —r [3-Butyl-5-(methoxymethyl) -lH-Pyrazol-4-yl) Imethyl] [1, l'- biphenyl]-2-yl]-2-(triphenylmethyl) -2H-tetrazole

Intermediate 5 (150mg) was stirred at room temperature under nitrogen with hydrazine hydrate (80% hydrazine hydrate; 0.018ml) in absolute ethanol (3ml) for 3 days. The solvent was evaporated and the residue purified by DFC, eluting with ether to give the title compound as a colourless gum (78mg) .

T.l.c. (ether) Rf 0.2.

Intermediate 10

(a) 5-[4'-[ [3-Butyl-5-(methoxymethyl)-1-(2,2,2-trifluoroethyl)-1H- pyrazol-4-yl]methyl] [1,1'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H- tetrazole; and

(b) 5-[4'-[[5-Butyl-3-(methoxymethyl)-1-(2,2,2-trifluoroethyl)-1 H- pyrazol-4-yl]methyl] [1,1'-biphenyl]-2-yl]-2-(triphenylmethyl)-2H- tetrazole

Intermediate 5 (0.805g) was stirred at room temperature with 2,2,2- trifluoroethylhydrazine (70% solution in water, 0.304ml) in ethanol for 21h. The solvent was evaporated and the residue purified by DFC eluting with System A (10:90 to 60:40) to give:

(a) Intermediate 10a, T.l.c (System A 50:50) Rf 0.35; and

(b) Intermediate 10b, T.l.c. (System A 50:50) Rf 0.2.

Intermediate 11

(a) 1, I'-Dimethylethyl 4' - [ [1- (2-amino-2-oxoethyl) -5-butyl-3- ( ethoxymethyl) - lH-py ra zol- 4 -yl] methyl] [1,1' -biphenyl] -2- carboxylate; -and

(b) 1, l'-Dimethylethyl 4' - [ [1- (2-amino-2-oxoethyl) -3-butyl-5- (methoxymethyl ) -lH-pyrazol-4-yl]methyl] [1, 1' -biphenyl] -2-carboxylate

Sodium hydride (52mg) was added to a stirred solution of Intermediate 6 (0.5g) in dry DMF (5ml), and stirring was continued at room temperature under nitrogen for l.Sh. Chloroacetamide (122mg) was added and stirring was continued at room temperature for 3h. The mixture was partitioned between water (30ml) and ethyl acetate (3x 20ml); the organic extracts were washed with 50:50 brine:water (3x30ml) , dried and evaporated to give a pale yellow gum (658mg) . Purification by DFC, eluting with System D (50:50) followed by ethyl acetate, and System E (95:5) gave:

(a) Intermediate 11a as a colourless oil (291mg) , T.l.c. (ethyl acetate) Rf 0.15; and

(b) Intermediate lib as a pale yellow gum (277mg) , T.l.c (ethyl acetate) Rf 0.3.

Intermediate 12

(a) 1, 1 ' -Dimethylet yl 4' -[ [5-butyl-3- (methoxymethyl) -1-methyl-lK-

pyrazol-4-yl]methyl] [1, l'biphenyl]-2-carboxylate; and (b) 1, 1' -Dimethylethyl 4' - f [3-butyl-5- (methoxymethyl) -1-methyl-lH- ρyrazol-4-yl ) methyl ] [1,1' -biphenyl ] -2-carboxylate

A mixture of Intermediate 6 (l.Og), methyl iodide (330mg) and potassium carbonate (320mg) in DMF (30ml) was stirred at room temperature for 3 days. The reaction mixture was poured into water and extracted with ethyl acetate. The combined organic extracts were washed with water, brine, dried and evaporated to give a colourless oil. This was chromatographed on silica gel eluting with System A (20:80 -. 80:20) followed by ether to give:

(a) Intermediate 12a as a colourless oil (124mg) , T.l.c (System F 99:1) Rf 0.3; and

(b) Intermediate 12b as a colourless oil (330mg) , T.l.c (System A 50:50) Rf 0.50.

Intermediate 13

(a) 1, 1-Dimethylethyl 4' - [ [5-butyl-l- f (dimethylamino) sulphonyl] -3-

( methoxymet yl ) -lH-pyrazol-4-yl] methyl]- [1, 1' -biphenyll-2- carboxylate; and

(b) 1, 1-Dimethylethyl 4'-[ [3-butyl-l- [ (dimethylamino) sulphonyl] -5- (methoxymethyl) -lH-pyrazol-4-yl]methyl] [1, 1' -biphenyl] -2-carboxylate Sodium hydride (52mg; 80% dispersion in oil) was added to a stirred solution of Intermediate 6 (0.5g) in dry DMF (5ml) at room temperature under nitrogen. After stirring for 0.5h, N,N- dimethylsulphamoyl chloride (0.185ml) was added at 0° and stirring was continued at 0° for 2h. The mixture was partitioned between water (30ml) and ethyl acetate (3x 20ml) ; the organic layers were washed with 50:50 brine:water (3x 30ml) and brine (30ml), dried and evaporated to give a pale yellow oil (0.76g) . Purification by DFC eluting with System A (20:80 to 40:60) gave:

(a) Intermediate 13a as a colourless oil (296mg), T.l.c. (System A 50:50) Rf 0.3; and

(b) Intermediate 13b as a colourless oil (231mg) , T.l.c. (System A 50:50) Rf 0.45.

Intermediate 14

(a) 5-[4'-[ [3-Butyl-l-ethyl-5- (methoxymethyl ) -lH-oyrazol-4- yllmethv] [1, 1' -biphenyl] -2-yl] -2- (tri ^p henylmethyl) -2H-tetrazole; and

(b) 5- [4' - [ [5-Butyl-l-ethyl-3- (methoxymethyl) -lH-pyrazol-4- yl]methy] [1, 1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole A solution of Intermediate 9 (1.26g) in dry DMF (5ml) was added dropwise to a suspension of sodium hydride (60% in oil, 116mg) in dry DMF (5ml) . The resulting pale yellow solution was stirred at room temperature for 0.5h, then a solution of the ethyl iodide (0.16ml) in dry DMF (1ml) was added dropwise. The mixture was stirred at room temperature for 2h, then partitioned between water (20ml) and ethyl acetate ( 3 x 20ml) . The combined organic extracts were washed with brine/water 1:1 (3x 50ml) and dried. The solvent was evaporated to give a pale yellow foam (1.283g) which was purified by short-path column chromatography on silica gel (Merck 7729) eluting with System C (1:1-.2:1) to give:

(a) Intermediate 14a as a pale yellow gum (569mg) n.m.r. (CDCI3, 250 MHz) δ 0.85 (3H,t), 1.2-1.6 (4H, m) , 1.40 (3H, t), 2.45 (2H, t) , 3.20 (3H, s) , 3.70 (2H, s) , 4.12 (2H, q) , 4.20 (2H, s), 6.90-7.50 (7K, m) , 7.85-7.9 (IH) ; and

(b) Intermediate 14b (654mg) n.m.r. (CDCI3, 250 MHz) δ 0.85 (3H, t) , 1.2-1.35 (4H, m) , 1.41 (3H, t), 2.40 (2H, t), 3.30 (3H, s) , 3.72 (2H, s) 4.05 (2H, q) , 4.28 (2H, s) , 6.90-7.50 (7H, m) , 7.85-7.9 (IH) .

Intermediate 15

(a) 5- [4' - [ [3-Butyl-l- [ (dimethylamino) sulohonoyl] -5- (methoxymethyl) -lH-pyrazol-4-yl] methyl] [1,1' -biphenyl] -2-yl] -2- ( triphenylmethyl) -2H-tetrazole; and

(b) 5-[4'-[ [5-Butyl-l-[ (dimethylamino) sulphonoyl]-3- (methoxymethyl) -lH-pyrazol-4-yl] methyl] [1,1' -biphenyl] -2-yl] -2- ( triphenylmethyl) -2H-tetrazole

Sodium hydride (60% in oil; 93mg) was added portionwise to a solution of Intermediate 9 (l.Og) in dry DMF (10ml) at room temperature under nitrogen. The resulting pale yellow suspension was stirred at room temperature for 0.5h, cooled to 0°C then a solution of dimethylsulphamoyl chloride (222mg) in dry DMF (0.5ml) was added dropwise. The resulting mixture was stirred at room temperature for 2h, then partitioned between ethyl acetate (3 x 20ml) and water (20ml) . The combined organic extracts were washed with brine/water (1:1) (3 x 20ml) and dried. The solvent was

evaporated to give a pale yellow foam (1.27g), which was purified by short-path column chromatography on silica gel (Merck 7729) eluting with System A (1:2) to give:

(a) Intermediate 15a as a colourless foam (462mg) T.l.c. (System C 1:1) Rf 0.4; and

(b) Intermediate 15b as a colourless foam (615mg) T.l.c (System C 1:1) Rf 0.35.

Intermediate 16 Phenylmethoxy-2,4-octandione

2-Hexanone (26.7g) was added dropwise over 5 rain at room temperature to a stirred suspension of sodium amide (10.4g) in dry ether (290ml) under nitrogen. The reaction mixture was stirred for 13min and a solution of methyl phenylmethoxyacetate (24.Og) in dry ether (40ml) was added dropwise over 12min. The mixture was then heated at reflux for 3h, cooled, and then poured into ice (400g) and 2N HCl (200ml) . The aqueous phase was extracted with ether (2 x 350ml) and the combined etheral solution washed with 8% sodium bicarbonate solution (2 x 250ml), water (200ml) and brine (200ml), then dried and evaporated to leave a yellow liquid (35.8g). This was purified by column chromatography on silica gel eluting with System A (2:98) to give the title compound as a yellow oil (10.95g) T.l.c. (System C 1:9) Rf. 0.50

Intermediate 17

1-[ (Phenylmethoxy)methyl]-3-[ [2'-[2- (triphenylmethyl)-2H-tetrazol-5- yl] [1,l'-biphenyl]-4-yl]methyl]-2, 4-octandione

To a suspension of sodium hydride (60% in oil; 820mg) in THF (63ml) at 0°C was added a solution of Intermediate 16 (4g) in THF (6.5ml) dropwise over 35min. The reaction mixture was stirred at room temperature for 40min prior to the addition of Intermediate 3 (6.38g) in THF (21ml) over 15 min. The resultant reaction mixture was heated at reflux for 17h. The reaction mixture was concentrated in vacuo and the residue partitioned between water (80ml) and ether (3x80ml) . The combined organic layers were dried and concentrated in vacuo and then purified by column chromatography on silica gel eluting with System C (1:8) to afford the title compound (3.0g) as a white solid.

T.l.c (System C 1:3) Rf 0.28

Intermediate 18

5- [ 4 ' - [ [ 3-Butyl-5- [ (phenylmethoxy) methyl ] -lH-pyrazol- - yljmethyl] [1,1'- biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole Hydrazine hydrate (85%; 0.85ml) was added to a solution of Intermediate 17 (10.17g) in ethanol (100ml) containing dichloromethane (20ml) . The mixture was stirred at room temperature for 2 days. The solvent was evaporated in vacuo and the residue purified by column chromatography on silica gel eluting with System C (4:1) and increasing the polarity to neat ether, to give the title compound as a colourless foam (7.67g) . T.l.c (ether) Rf 0.5.

Intermediate 19

Phenylmethoxy-2,4-heptandione

From 2-heptanone and methyl phenylmethoxyacetate according to the method of Intermediate 16.

T.l.c. System A (1:10) Rf 0.39

Intermediate 20

1-[ (Phenylmethoxy)methyl]-3-[[2'-[2-(triphenylmethyl)-2H-tetraz ol-5- yl] [1,1'-biphenyl]-4-yl]methyl] -2,4-heptandione

From Intermediate 19 according to the method of Intermediate 17.

T.l.c. System A (1:4) Rf 0.20

Intermediate 21

5-[4' -[ [3-Propyl-5-[ (phenylmethoxy) methyl] -lH-pyrazol- 4- yl]methyl] [1, 1' -biphenyl] -2-yl] -2- ( riphenylmethyl) -2H-tetrazole From Intermediate 20 according to the method of Intermediate 18. T.l.c. System A (5:1) Rf 0.25

Intermediate 22

(a) 5-f 4' - [ [3-Butyl-l-ethyl-5- [ (phenylmethoxy) methyl ] -lH-pyrazol-4- yl]methyl] [1, l'-biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole; and

(b) 5-[4'-[[5-Butyl-l-ethyl-3-[ (phenylmethoxy) methyl] -lH-pyrazol-4- yl]methyl] [1, 1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole

A solution of Intermediate 18 (3.0g) in dry DMF (15ml) was added dropwise to a suspension of sodium hydride (60% dispersion in oil; 254mg) , in dry DMF (15ml) at 0 ^* under nitrogen. The mixture was stirred at 5-10 ^* for 15mins, then a solution of ethyl iodide (660mg) in dry DMF (1ml) was added dropwise. The mixture was allowed to warm to room temperature over lh . The mixture was partitioned between water (50ml) and ethyl acetate (3x25ml) and the combined organic extracts washed with brine/water 1:1 (2x50ml) and dried. The solvent was evaporated to give a pale yellow gum (3.19g) which was purified by column chromatography on silica gel eluting with System A (1:2-.1:1) to give the title compound:

(a) Example 22a as a colourless foam (1.33g), T.l.c System A (1:2) Rf 0.2; and

(b) Example 22b as a colourless foam (1.60g), T.l.c. System A (1:2) Rf 0.5.

Similarly prepared from Intermediate 18 and the appropriate alkyl halide were:-

Intermediates 23a and b

(a) 5- [4' -[ [3-Butyl-l- (1-methylethyl) -5-[ (phenylmethoxy)methyl]-1H- pyrazol-4-yl]methyl] [1,1' -biphenyll-2-yl]-2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (1:2) Rf 0.45

(b) 5- [4' - [ [5-Butyl-l- (1-methylethyl) -3-[ (phenylmethoxy)methyl] -1H- ρyrazol-4-yl]methyl] [1, 1' -biphenyl]-2-yl]-2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (1:2) Rf 0.35

Intermediates 24a and b

(a) 5-[4'-[ [3-Butyl-l-(2-methylpropyl)-5-[ (phenylmethoxy)methyl]-1H- pyrazol-4-yl]methyl] [1,1' -biphenyl]-2-yl]-2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (2:3) Rf 0.3

(b) 5- [ 4 ' - [ [5-Butyl-l- (2-methylpropyl) -3- [ (phenylmethoxy) methyl] -1H- pyra zol- -yl ] met yl ] [ 1 , 1 ' -biphenyl ] -2-yl] -2- (t riphenylmethyl ) -2H- tetrazole

T . l . c . System A ( 1 : 2 ) Rf 0 .25

Intermediates 25a and b

(a) 5- [4* - [ [3-Butyl-l- (2-cvclopropylmethyl) -5- [ (phenylmethoxy) methyl] -lH-pyrazol-4-yl] methyl] [1,1' -biphenyl] -2-yll -2-

(triphenylmethyl) -2H-tetrazole T.l.c. System A (2:3) Rf 0.45

(b) 5- [4 ' - [ [ 5-Butyl-l- (2-cvclopropylmethyl) -3- [ (phenylmethoxy) methyl] -IH- p ra zol- 4 -yl] methyl] [1, 1' -biphenyl] -2-yl] -2-

( triphenylmethyl) -2H-tetrazole T.l.c. System A (2:3) Rf 0.42

Intermediates 26a and b

(a) 5-[4'-[ [3-Butyl-l-cvclobutyl-5-[ (phenylmethoxy) methyl] -1H- pyrazol- 4-yl] methyl] [1,1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (1:1) Rf 0.71

(b) 5-[4'-[ [5-Butyl-l-cvclobutyl-3-[ (phenylmethoxy) methyl] -1K- p yrazo 1 -4-yl] methyl] [1, l'-biphenyl] -2-yl] -2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (1:1) Rf 0.68

Intermediates 27a and b

(a) 5-[4'-[ [3-Butyl-l-methyl-5-[ (phenylmethoxy)methyl]-lH-pyrazol-4- yl]methyl] [1, 1'-biphenyl]-2-yl]-2-(triphenylmethyl)-2H-tetrazole T.l.c. System A (4:1) Rf 0.44

(b) 5-[4'-[ [5-Butyl-l-methyl-3-[ (phenylmethoxy)methyl]-lH-pyrazol-4- yl]methyl] [1,l'-biphenyl]-2-yl]-2-(triphenylmethyl)-2H-tetrazole T.l.c. System A (4:1) Rf 0.34

Intermediates 28a and b

(a) 5-[4'-[ [3-Butyl-l-proρyl-5- [ (phenylmethoxy)methyl]-lH-pyrazol-4- yl]methyl] [1,1'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H-tetrazole T.l.c. System A (2:1) Rf 0.60

(b) 5-[4'-[ [5-Butyl-l-propyl-3-[ (phenylmethoxy)methyl]-lH-pyrazol-4- yl]methyl] [1,l'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H-tetrazole T.l.c. System A (2:1) Rf 0.44

Intermediates 29a and b

(a) 5- [4' - [ f1, 3-Dibutyl-5- [ (phenylmethoxy)methyl] -lH-pyrazol-4- yljmethyl] [1, 1'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H-tetrazole T.l.c. System G (60:30:1) Rf 0.80

(b) 5- [4' - [ [1, 5-Dibutyl-3- [ (phenylmethoxy)methyl] -lH-pyrazol-4- yl]methyl] [1, 1'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H-tetrazole T.l.c. System G (60:30:1) Rf 0.66

Similarly prepared from Intermediate 21 and the appropriate alkyl halide were:-

Intermediates 30a and b

(a) 5-[4'-[ [l-Ethyl-3-proρyl-5-[ (phenylmethoxy)methyl]-lH-pyrazol-4- yl]methyl] [1, 1'-biphenyl]-2-yl] -2- (triphenylmethyl) -2H-tetrazole T.l.c. System A (3:1) Rf 0.75

(b) 5-[4' - [ [l-Ethyl-5-ρropyI-3- [ (phenylmethoxy)methyl]-lH-pyrazol-4- yl]methyl3 [1, 1' -biphenyl]-2-yl]-2- (triphenylmethyl) -2H-tetrazole T.l.c. System A (3:1) Rf 0.60

Intermediates 31a and b

(a) 5-[4'- [ [1- (1-Methylethyl) -3-propyl-5-[ (phenylmethoxy)methyl]-1H- pyrazol-4-yl]methyl] [1, 1' -biphenyl]-2-yl]-2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (1:1) Rf 0.50

(b) 5-[4'-[ [1- (1-Methylethyl) -5-propyl-3-[ (phenylmethoxy)methyl]-1H- pyrazol-4-yl]methyl] [1,1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H- tetrazole

T.l.c. System A (1:1) Rf 0.36

Intermediate 32 Phenylmethoxy-2, -hexandione

2-Butanone (47.65ml) was added dropwise over lOmin to a mechanically stirred suspension of sodium amide (95% ; 20.78g) dry ether (600ml) at room temperature under nitrogen . The reaction mixture was st irred at room temperature f or 15min . A s olution of methyl phenylmethoxyacetate ( 48. Og) in dry ether (50ml) was added dropwise over 13min at room temperature under nitrogen , and the reaction progressed according to the method of Intermediate 16, to give the title compound as a yellow oil (23.35g)

T.l.c. System A (1:10) Rf 0.15.

Intermediate 33 l-[ (Phenylmethoxy)methyl]-3-[ [2-[2-(triphenylmethyl) -2H-tetrazol-5- yl] [1,1'-biphenyl3-4-yl]methyl]-2, -hexandione

From Intermediate 32 according to the method of Intermediate 20.

T.l.c. System A (1:4) Rf 0.30.

Intermediate 34

5-r4'-[[3-Ethyl-5-[ (phenylmethoxy) methyl] -lH-pyrazol-4- yl] methyl] [1, 1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole From Intermediate 33 according to the method of Intermediate 21. T.l.c. System A (6:1) Rf 0.21.

Intermediates 35a and b

(a) 5- [4' - [ [1, 3-Diethyl-5- [ (phenylmethoxy) methyl] -lH-pyrazol-4- yl]methyl] [1, 1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole; and

(b) 5-[4'-[ [l,5-Diethyl-3-[ (phenylmethoxy) methyl] -lH-pyrazol-4- yl]methyl] [1,1' -biphenyl] -2-yl] -2- (triphenylmethyl) -2H-tetrazole From Intermediate 34 according to the method of Intermediate 22 gave:

(a) Intermediate 35a as a yellow gum (l.lδg) T.l.c. System A (3:1) Rf 0.60

(b) Intermediate 35b as a yellow gum (1.40g) T.l.c. System A (3:1) Rf. 0.40.

Intermediate 36

1, l-Dimethylethyl 4' - [3-oxo-2- (phenoxyacetyl) heotanyl] [1, l'- biphenyl] -2-carboxylate

From Intermediate 16 and Intermediate 2 according to the method of Intermediate 17. T.l.c. System A (1:5) Rf 0.25.

Intermediate 37

1, 1-Dimethylethyl 4'-[ [3-butyl-5-[ (phenylmethoxy) methyl] -lH-pyrazol-

4-yl 3 methyl] [1, 1' -biphenyl] -2-carboxylate

From Intermediate 36 according to the method of Intermediate 18.

T.l.c. System A (3:2) Rf 0.15.

Intermediate 38a and b

(a) 1, 1-Dimethylethyl 4' - [ [3-butyl-l-ethyl-5- [ (phenylmethoxy) methyl ] -lH-pyrazol- -yl 3 methyl 3 [1,1' -biphenyl 3 -2 -carboxylate ; and

(b) 1, 1-Dimethylethyl 4' - [ [5-butyl-l-ethyl-5- [ (phenylmethoxy) methyl] -lH-pyrazol-4-yl]methyl] [1,1' -biphenyl] -2-carboxylate

From Intermediate 37 and ethyl iodide according to the method of

Intermediate 22.

Intermediate 38a: T.l.c. System A (1:1) Rf 0.35

Intermediate 38b: T.l.c. System A (1:1) Rf 0.2.

Intermediate 39a and b

(a) 1,1-Dimethylethyl 4' -[[3-butyl-l- (1-methylethyl) -5- [ (phenylmethoxy) methyl ] -lH-ρyrazol-4-yl] methyl] [1,1' -biphenyl] -2- carboxylate; and

(b) 1, 1-Dimethylethyl 4'-[ [5-butyl-l- ( 1 -met hylet h yl ) -3- [ (phenylmethoxy) methyl ] -lH-pyrazol-4-yl] methyl] [1,1' -biphenyl] -2- carboxylate

From Intermediate 37 and isopropyl iodide according to the method of

Intermediate 22.

Intermediate 39a: T.l.c. System A (1:2) Rf 0.3

Intermediate 39b: T.l.c. System A (1:2) Rf 0.2.

Intermediate 40

1, 1-Dimethylethyl 4' - [ [3-butyl-l-ethyl-5- [ (hydroxy ethyl) -1H- pyrazol-4-yl]methyl] [1, 1' -biphenyl] -2-carboxylate

From Intermediate 38a according to the method of Example 26.

T.l.c. ethyl acetate :hexane (1:1) Rf. 0.4.

Intermediate 41

1, 1-Dimethylethyl 4'-[ [3-butyl-5- (hydroxymethyl) -1- (1-methylethyl) - lH-pyrazol-4-yl3methyl] [1, 1' -biphenylj -2-carboxylate

From Intermediate 39a according to the method of Example 26.

T.l.c. System A (1:1) Rf. 0.25.

Intermediate 42

1, 1-Dimethylethyl 4' - [ [3-butyl-l-ethyl-5-formyl-lH-pyrazol-4- yl3methyl3 [1,1'-biphenyl]-2-carboxylate

From Intermediate 40 according to the method of Example 42.

T.l.c. System A (1:1) Rf 0.7.

Intermediate 43

1 , 1-Dimethylethyl 4 ' - [ [ 3-butyl-5-f ormyl-1- ( 1-methylethyl ) -1H- pyrazol-4-yl] methyl] [1, l' -biphenyl] -2-carboxylate

From Intermediate 41 according to the method of Example 42.

T. l .c . System A (1: 1) Rf . 0.7.

Intermediate 44

3-Butyl-4-[ [2'-[ (1,1-dimethylethoxy) carbonyl] [1, 1' -biphenyl] -4- yllmethyl]-l-ethyl-lH-pyrazole-5-carboxylic acid

From Intermediate -42 according to the method of Example 50.

T.l.c. ether:hexane:acetic acid (50:50:1) Rf. 0.6

Intermediates 45a and b

(a) 5-[4'-[ [1-(2-Methylpropyl) -3-propyl-5-[ (phenylmethoxy)methyl]- lH-pyrazol-4-yl]methyl] [1, 1'-biphenyl]-2-yl]-2-(triphenylmethyl)-2H- tetrazole; and

(b) 5-[4'-[ [1-(2-Methylpropyl) -5-propyl-3-[(phenylmethoxy)methyl]- IH-pyrazol- -yl]methyl] [1,1'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H- tetrazole

From Intermediate 21 and 2-methylpropyl iodide according to the method of Intermediate 22.

Intermediate 45a: T.l.c. System A (1:1) Rf 0.27

Intermediate 45b: T.l.c. System A (1:1) Rf 0.19

Intermediate 46

3-Butyl-4-[ [2'-(l, 1-dimethylethoxy) carbonyl] [1, 1' -biphenyl] -4- yl] ethyl]-1-(1-methylethyl)-lH-pyrazole-5-carboxylic acid

From Intermediate 43 according to the method of Example 50.

T.l.c. System G (20:20:1) Rf 0.65

Example 1

4' - [ [3-Butyl-5- (methoxymethyl) -1H-pyrazo1-4-yl]methyl] [1,1'- biohenyl]-2-carboxylic acid trifluoroacetate (1:1) salt

A solution of Intermediate 6 (320mg) in dry dichloromethane (30ml) was treated with TFA (1ml) and the reaction stirred at roo temperature for 5h. The volatiles were removed in vacuo and th residue triturated with ether to give the title compound (162mg) a a white solid.

T.l.c. (System C 50:50), Rf 0.05.

Assay Found: C, 60.7; H,5.7; N, 5.5.

^C 23 ^H 26 ^N 2°3 - ^CF 3 ^C0 2 ^H re * ¹ ____ ^• <=_. C, 61.0; H,5.5; N,5.7%.

Example 2

4'-[ [5-Butyl-3- (methoxymethyl) -l-phenyl-lH-pyrazol-4-yl]methyl] [1, 1' -biphenyl] -2-carboxylic acid

A solution of Intermediate 7a in TFA (3ml) was stirred at 20° for 90min. The reaction mixture was concentrated in vacuo and the residue was dissolved in dichloromethane (15ml) . The organic layer was extracted with sodium bicarbonate solution (8%; 3x 30ml) . The combined extracts were acidified to pH=l with dilute hydrochloric acid and then extracted with dichloromethane (3x 30ml) . The combined extracts were washed with brine (30ml), dried and concentrated _in vacuo to yield a the title compound as a pale brown solid (157mg) . m.p. 85-91°C T.l.c. (System B 50:8:1) Rf 0.44.

Example 3

4'-[ [3-Butyl-5-(methoxymethyl)-l-phenyl-lH-pyrazol-4yl3methyl3 [1,1'- biphenyl3-2-carboxylic acid

Intermediate 7b' (256mg) was stirred at room temperature with TFA (lml) in dry dichloromethane (5ml) overnight. The solvent was evaporated to give a brown oil (280mg) . Purification by DFC eluting with System G gave the title compound as a pale yellow gum (95mg) . T.l.c. (System G 50:50:1) Rf 0.4 Analysis Found: C,76.6; H,6.9; N,5.7. ^C 29 ^H 30 ^N 2°3 requires C,76.6; H,6.65 N,6.2%

Example 4

4' -f f3-Butyl-5-(methoxymethyl)-1-(2,2,2-trifluoroethyl) -IH-pyrazo -4-yl3methyl] [1, 1'-biphenyl]-2-carboxylic acid

Intermediate 8a (180mg) was dissolved in 98% formic acid (4ml) and the solution allowed to stand at room temperature overnight (16h) . The excess formic acid was evaporated and the residue azeotroped with toluene (20ml) to give a colourless gum (175mg) . Purification by DFC eluting with System G (50:50:1) gave the title compound as a colourless gum (90mg) . T.l.c (System G 50:50:1) Rf 0.5. Analysis Found: C, 65.5; H,5.9; N, 6.1.

^C 25 ^H 27 ^F 3 ^N 2°3 requires C, 65.2; H,5.9; N, 6.1%.

Example 5

4'-[[5-Butyl-3-(methoxymethyl)-1-(2,2,2-trifluoroethyl)-l H-pyrazol'

-4-yl3methyl3 [1,l'-bipheny!3-2-carboxylic acid

Intermediate 8b was treated according to the method of Example 4 to give the title compound as a colourless gum (89mg) .

T.l.c (System F 99:1) Rf 0.75.

Analysis Found: C,65.5; H, 6.2; N,5.9.

^C 25 ^H 27 ^F 3 ^N 2°3 requires C,65.2; H-5.9; N, 6.1%.

Example 6

5- [4' -[ [3-Butyl-5- (methoxymethyl) -lH-pyrazol-4-yl3methyl3 [1,1'- biphenyl3 -2-yl] -lH-tetrazole

Intermediate 9 (40mg) was treated with 2N hydrochloric acid (1ml) in

THF (5ml) at room temperature and the mixture was stirred at room temperature for 7h . The mixture was partitioned between ethyl acetate (15ml) and dilute aqueous sodium hydroxide (2ml of 2N in

15ml of water) . The aqueous layer was neutralized with excess saturated aqueous ammonium chloride (10ml) and extracted with ethyl acetate (3x 10ml) . The latter organic layers were washed with brine, dried and evaporated to give the title compound as a colourless gum (12mg) . T.l.c. (System H 99:1) Rf 0.3. n.m.r. (CDC1 ₃ ,250 MHz) δ, 0.85 (3H, t), 1.15-1.55 (4H, m) ,

2.45 (2H, t) , 3.2 (3H, s) , 3.77 (2H, s), 4.14 (2H, s) , 7.0-8.0

(8H, m) , 8.3 (2H, broad s).

Example 7

5-[4'-[ [3-Butyl-5- (methoxymethyl) -l-(2,2, 2-trif luoroethyl) -1H- oyrazol-4-yl] methyl] [1, 1' -biphenyl] -2-yl] -lH-tetrazole

dl-10-Camphor sulphonic acid (20mg) was added to a stirred solution of Intermediate 10a (103mg) in THF (10ml) and methanol (3ml), an stirring was continued at room temperature for 16h. The solvent was evaporated to give a pale yellow oil, which was purified by DFC eluting with System G to give the title compound as a white solid (39mg) . m.p. 45-51°C T.l.c. (System F 99:1) Rf 0.5.

Example 8

5-[4'-[ [5-Butyl-3-(methoxymethyl)-1-(2,2,2-trifluoroethyl)-1H- pyrazol-4-yl3methyl3 [1,1'-biphenyl3-2-yl3-lH-tetrazole dl-10-Camphor sulphonic acid (40mg) was added to a stirred solution of Intermediate 10b (203mg) in THF (20ml) and methanol (6ml), and stirring was continued at room temperature for 20h. The solvent was evaporated to give a pale yellow oil. Purification by DFC eluting with System G (50:50:1 to 100:0:1) gave a colourless oil (129mg) which was dissolved in chloroform (10ml) . n-Heptane was added, and the solvents evaporated to give the title compound as a white foam (lOlmg) m.p. 48-50°. T.l.c. (System F 99:1) Rf 0.4.

Example 9

4'-[ [l-(2-Amino-2-oxoethyl)-5-butyl-3-(methoxymethyl)-lH-pyrazol -4- yl3 ethyl3 [1, 1'-biphenyl3-2-carboxylic acid

Intermediate 11a was treated according to the method of Example 4 to give the title compound as a white solid (170mg), m.p. 204-206°,

T.l.c. (System H 98:2) Rf 0.15 .

Example 10

4'-[ n-(2-Amino-2-oxoethyl)-3-butyl-5-(methoxymethyl)-lH-pyrazol- 4- yl]methyl] [1, l'-biphenyl]-2-carboxylic acid

Intermediate lib was treated according to the method of Example 4 to give the title compound as a white solid (126mg) , m.p. 179-180°

T.l.c (System H 98:2) Rf 0.3.

Example 11

4'-[ [5-Butyl-3-(methoxymethyl) -l-methyl-lH-pyrazol-4-yl]methyl3 [1,1'

-biphenyl3-2-carboxylic acid

A solution of Intermediate 12a (114mg) in dry DMF (5ml) was treated with TFA (1ml) at room temperature, and stirring was continued at room temperature for 19h. The solvent was evaporated to give a pale yellow oil, which was azeotroped with n-heptane to give a yellow oil. Purification by DFC eluting System F (98:2) gave a pale orange oil (llOmg) , which was azeotroped with n-heptane to give the title compound as a light brown foam (78mg) m.p. 45-51°. T.l.c. (System F 99:1) Rf 0.2.

Example 12

4'- [ [3-Butyl-5- (met oxymethyl ) -1 -methyl- lH-pyrazol-4- yl] methyl] [1, l'-biphenyl] -2-carboxylic acid, trifluoroacetate (1:1) salt

Trifluoroacetic acid (1ml) was added to a stirred solution of Intermediate 12b (310mg) in dry DMF (10ml) at room temperature, and stirring was continued at room temperature for 27h. The solvent was evaporated to give a pale yellow oil (470mg) . n-Heptane (20ml) was added and evaporated; this was repeated twice to give the title compound as a pale yellow oil (263mg) . T.l.c (Ether) Rf 0.25. Assay Found: C, 61.9; H, 6.0; N,5.6.

^C 24 ^H 28 ^N 2 ⁰ 3_ ^CF 3 ^COOH requires C, 61.65; H,5.8; N,5.5%.

Example 13

4' - [ [5-Butyl-l- [ (dimethylamino) sulphonyl] -3- (methoxymethyl) -1H- pyrazol-4-yl3methyl3 [1,1' -biphenyl 3 -2-carboxylic acid Intermediate 13a (221mg) was dissolved in 98% formic acid (4ml) and the solution allowed to stand at room temperature for 6h. The excess formic acid was evaporated and the residue azeotroped twice with toluene (2x 15ml) to give a colourless gum (250mg) . Purification by DFC eluting with System A (50:50) and System G ^■ (50:50:1) gave the title compound as a colourless gum (169mg) . T.l.c. (System F 99:1) Rf 0.7 Analysis Found: C, 61.5; H,6.4; N, 8.7.

^C 25 ^H 31 ^N 3°5 ^{S re} - ^ires C, 61.8 ^; H, 6.4; N, 8.65%.

Example 1

4' - [ [3-Butyl-l- [ (dimethylamino) sulphonyl] -5- (methoxymethyl) -1H- pyrazol-4-yl] methyl] [1, 1' -biphenyl] -2-carboxylic acid

Intermediate 13b was treated according to the method of example 13 to give the title compound as a white foam (lllmg) .

T.l.c. (System F 99:1) Rf 0.8.

Analysis Found: C,62.0; H,6.5; N, 8.4.

^C 25 ^H 31 ^N 3°5 ^S requires C,61.8; H,6.4; N,8.65%.

Example 15

5- r ' - [ [3-Butyl-l-ethyl-5- (methoxymethyl) -lH-pyrazoI-4- yl3methyl] [1, 1' -bipheny!3 -2-yl] -lH-tetrazole

Concentrated HCl (0.4ml) was added dropwise to a solution of Intermediate 14a (0.542g) in methanol (10ml) containing THF (2ml)-. The mixture was stirred at room temperature under nitrogen for lh, then basified to pH 10 with sodium hydroxide solution (2N; 3ml) . The solvent was concentrated in. vacuo and the residue diluted with water (20ml). The mixture was extracted with ether (3 x 20ml), then the aqueous phase acidified to pH 1 with HCl (2N; 4ml) . The resulting opaque solution was extracted with ethyl acetate (3 x 30ml) and the combined ethyl acetate fractions washed with brine (1 x 30ml) and dried. The solvent was evaporated iji vacuo to give a colourless foam (0.34g) which was purified by column chromatography on silica gel eluting with ethyl acetate/petroleum ether (40- 60°) (2:1) to give the title compound as a colourless foam (304mg) n.m.r. (MeOD, 250 MHz) δ 0.85 (3H, t) , 1.2-1.5 (6H, m) , 2.45 (2H, t), 3.25 (3H, s) , 3.80 <2H, s) , 4.13 (2H, q) , 4.38 (2H, s) , 7.04 (4H, dd) , 7.5-7.68 (4H, m) . T.l.c. (ether/petroleum ether/acetic acid 50:20:0.2) Rf 0.2.

Similarly prepared:

Example 16

5- [4' - [ [5-Butyl-l-ethyl-3- (methoxymethyl) -lH-pyrazol-4- yll methyl] [1, l ^f -biphenyl] -2-yl-lH-tetrazole as a colourless foam (373mg) . n.m.r. (MeOD, 250 MHz) δ 0.85 (3H, t) , 1.3-1.4 (6H, m) , 2.55 (2H, t), 3.25 (3H, s), 3.80 (2H, s) , 4.05 (2H, q) , 4.28 (2H, s), 7.05 (4H, q), 7.5-7.68 (4H, m) . T.l.c (ether/petroleum ether/acetic acid 50:20:0.2) Rf 0.15.

From the dropwise addition of concentrated HCl (0.4ml) to a solution of Intermediate 14b (634mg) in methanol (10ml) and THF (2ml) .

Example 17

5-[4'-[ [3-Butyl-l- [ (dimethylamino) sulphonoyl] -5— (methoxymethyl) -1H- pyrazol-4-yl] methyl] [1,1' -biphenyl] -2-yl ] -IH-tet razole as a colourless foam (288mg) . n.m.r. (dg-DMSO, 250 MHz) δ 0.80 (3H, t) , 1.6-1.30 (2H,m), 1.37-1.49 (2H,m), 2.40 (2H, t) , 2.90 (6H, s) 3.28 (3H, s) , 3.83 (2H, s) , 4.60 (2H, s), 7.0-7.1 (4H, dd) , 7.46-7.68 (4H, m) . T.l.c (ethyl acetate) Rf 0.7.

From the dropwise addition of concentrated HCl (0.4ml) to a solution of Intermediate 15a (430mg) in methanol (10ml) and THF (2ml) .

Example 18

5-[4'- [ [5-Butyl-l- [ (dimethylamino) sulphonoyl] -3- (methoxymethyl) -1H- pyrazol-4-yl] methyl] [1,1' -biphenyl] -2-yl] -IH-tet razole as a colourless foam (382mg) . n.m.r. (dg-DMSO, 250 MHz) δ 0.82 (3H, t) , 1.17-1.40 (4H,m) , 2.76

(2H, t), 2.95 (6H, s) _r 3.20 <3H, s) , 3.80 (2H, s) 4.20 (2H, s) 7.05

(4H, dd), 7.48-7.68 (4H, m) .

T.l.c (ethyl acetate) Rf 0.7.

From the dropwise addition of concentrated HCl (0.5ml) to a solution of Intermediate 15b (579mg) in methanol (10ml) and THF (2ml) .

Example 19

(a) 5-[4'-π3-Butyl-5-[ (phenylmetho y) methyl] -1- (2, 2, 2- trif luoroethyl) -lH-pyrazol-4-yl] methyl] [1, l'-biohenyl] -2-yl] -1H- tetrazole; and

(b) 5-[4'-[ [5-Butyl-3-[ (phenylmethoxy) methyl] -1- [2,2, 2- trif luoroethyl ) -lH-ρyrazol-4-yl] methyl] [1, 1' -biphenyl] -2-yl] -1H- tetrazole

A solution of Intermediate 17 (2.6g) and 2, 2, 2-trif luoroethyl- hydrazine (70% in water, 584mg) in ethanol (75ml) and dichloromethane (25ml) was heated at reflux for 3h at which point a further quantity of 2, 2, 2-trifluoroethylhydrazine (300mg) was added and the resultant solution heated at reflux for 16h. Concentrated HCl (1ml) was added to the cooled reaction mixture and the resultant

solution stirred at room temperature for 4h. The reaction mixture was then adjusted to pH 10 (5N NaOH aq) and the solvents removed in vacuo. The residue was partitioned between water (100ml) and ether (3 x 100ml) and the aqueous phase was acidified to pH 5 (2N HCl aq) and extracted into ethyl acetate (3 x 100ml) . The combined organic extracts were dried, concentrated i_n vacuo and purified by column chromatography on silica gel eluting with petroleum ether.-ether:methanol:acetic acid (66:34:1:1) to afford:

(a) Example 19a as a white solid (930mg),

T.l.c (petroleum ether:ether:methanol:acetic acid 66:34:1:1) Rf. 0.5 n.m.r. (CDCI3, 250MHz) δ 0.85 (3H, t) , 1.28 (2H, sex), 1.51 (2H, pent), 2.48 (2H, t) 3.8 (2H, s) , 4.46 (4H, 2 x s) , 4.68 (2H, q) , 7.2-7.6 (12H,m), 8.1 (IH, d) ; and

(b) Example 19b as a white solid (892 mg) .

T.l.c (petroleum ether:ether:methanol:acetic acid 66:34:1:1)

Rf 0.22 n.m.r. (CDCI3, 250MHz) δ 0.9 (3H, t) , 1.3 - 1.5 (4H,m) , 2.62 (2H, t), 3.86 (2H, s), 4.40 (2H, s), 4.42 (2H, s) , 4.62 (2H, q) , 7.0 -7.6

<12H,m), 8.12 (IH, dd) .

Example 20

5- [4' - [ [3-Butyl-l- (2,2,2-trifluoroethyl) -lH-pyrazol-4- v!3methyl3 [1, 1'-biphenyl3-2-yl3-lH-tetrazole-5-methanol

A suspension of palladium on charcoal (10%, 32mg) in ethanol (5ml) was pre-treated with hydrogen and then a solution of the product of

Example 19a (170mg) in ethanol (3ml) was added and the resultant mixture was stirred vigorously under an atmosphere of hydrogen for

16h. The catalyst was removed by filtration and the filtrate concentrated in vacuo. This crude product was purified by column chromatography on silica gel eluting with chloroform/methanol (20:1) to afford the title compound (90mg) as a white solid. n.m.r. (CDCI3, 400MHz) δ 0.90 (3H, t) , 1.25 (2H, sex), 1.52 (2H, pent), 2.52 (2H, t) 3.0-4.0 (2H, br) , 3.82 (2H, s), 4.52 (2H, s) ,

4.76 (2H, dd), 6.9-7.6 (7H,m), 8.18 (lH,d).

I.r. (CHCI3) cm ^"1 3464 (s) , 2873 (m) , 1717 (w) 1390 (m) .

Similarly prepared:-

Example 21

5- T 4 ^r - [ [5-Butyl-3- (hydroxymethyl ) -1- (2 , 2 , 2-trif luoroethyl) -1H- pyrazol-4-yl] methyl] fl, l'-biphenyl] -2-yl] -lH-tetrazole as a white solid (430g) .

T.l.c (petroleum ether: ether :methanol: acetic acid 50:50:3:3) Rf 0.15 From a solution of the product of Example 19b (450mg) in ethanol (10ml) . n.m.r. (dg-DMSO, 250MHz) 0.80 (3H,t) , 1.20 (4H,m), 2.50 (2H,m) , 3.81 (2H,s), 4.31 (2H,s), 5.00 (2H,dd), 7.00 (2H,d), 7.12 (2H,d), 7.5-7.7 (4H,m) .

Example 22

3-Butyl-4-[[2'-(lH-tetrazol-5-yl) [1, 1' -biphenyl 3 -4-yl] methyl] -1- (2,2, 2-trif luoroethyl) -lH-pyrazole-5-carboxaldehvde

Tetra-n-propylammonium perruthenate (2mg) was added to a mixture of the product of Example 20 (26.5mg), 4-methylmorpholine N-oxide (lOmg) and powdered 4A molecular sieves (152mg) in a mixture of dry dichloromethane (2ml) and dry acetonitrile (2ml) at room temperature under nitrogen. The resultant mixture was stirred at room temperature for 10 min, then the solvent evaporated in vacuo. The residue was purified by short -path column chromatography on silica gel eluting with ether/petroleum ether/acetic acid (70:35:1) to give the title compound as a pale purple foam (18mg) n.m.r. (CDCI3; 250 MHz) δ 0.9 (3H, t) , 1.3-1.4 (2H,m) , 1.5-1.6 (2H,m), 2.58 (2H, t) , 4.15 (2H, s) , 5.18 (2H, q) , 7.18-7.65 (7H,m), 8.15 (IH, d), 9.85 (IH, s) . T.l.c- (ether/acetic acid 100:1) Rf. 0.35

Similarly prepared: -

Example 23

5-Butyl-4-[ [2'-(lH-tetrazol-5-yl) [1, 1' -biphenyl] -4-yl]methyl3 -1-

(2,2, 2-trif luoroethyl) -lH-pyrazole-3-carboxaldehvde as a white solid

(20.6mg) .

T.l.c. (petroleum ether:ether:methanol:acetic acid 25:25:1:1) Rf 0.6. n.m.r. (CDCI3, 250MHz) , 0.76 (3H,t) , 1.1-1.3 (4H,m), 2.45(2H,t), 2.90(2H,s), 4.50 (2H, q) , 6.8-7.3 (7H,m) , 8.0 (lH,d),9.75 (lH,s).

From the addition of tetra-n-p ropy lammonium perruthenoate (4mg) to a stirred mixture of the product of Example 21 (104mg), N- methylmorpholine N-oxide (38.9mg) and freshly powdered 4A molecular seives (l.lg) in dry dichloromethane (2.5ml) and dry acetonitrile (2.5ml) .

Example 24

3-Butyl-4-[ [2'-(lH-tetrazol-5-yl) [1, 1' -biphenyl 3 -4-yl 3 methyl ] -1- (2,2, 2-trif luoroethyl) -lH-pyrazole-5-carboxylic acid

A solution of sodium chlorite (325mg; 80%) and sodium dihydrogen phosphate (325mg) in water (3ml) was added to a solution of the product of Example 22 (168mg), 2-methyl-2-butene (2.14ml;2M in THF), tert-butanol (4ml) in THF (3ml) at room temperature and the mixture stirred for 15 min under nitrogen. The solvent was evaporated and the residue was partitioned between water (10ml) and ethyl acetate (3 x 10ml) . The combined organic extracts were dried and evaporated to give a pale purple foam (242mg) . The crude material was purified by short-path column chromatography on silica gel eluting with ether/petroleum ether/acetic acid (50:50:1) to give the title compound as a colourless foam (91mg) .

T.l.c (ether/petroleum ether/acetic acid 50:50:1) Rf 0.2. n.m.r. (MeOD; 250 MHz) δ 0.88 (3H, t) , 1.1-1.5 (4H,m), 2.49 (2H, t) , 4.15 (2H, s), 5.25 (2H, q) 7.05 (4H, dd) , 7.50-7.70 (4H,m) .

Similarly prepared: -

Example 25

5-Butyl-4-[ [2'-(lH-tetrazol-5-vD [1, 1'-biphenyl3-4-yl]methyl3 -1-

(2,2,2-trifluoroethyl) -lH-ρyrazole-3-carboxylic acid as a white solid (44mg) .

T.l.c. (ether:petroleum ether:ethanol:acetic acid, 25:25:1:1) Rf

0.12. n.m.r. (dg-DMSO, 00MHz) δ 0.81 (3H, t) , 1.24 (4H, br),2.62 (2H, t) ,

4.05 (2H, s), 5.12 (2H,q), 6.98 (4H, dd) , 7.3-7.6 (4H, m) .

From a solution of sodium chlorite (171mg; 80%) and sodium dihydrogen phosphate (171mg) in water (1.5ml) added to a stirred solution of the product of Example 23 (88.5mg), 2-methylbut-2-ene

(2M in THF, 1.13ml), tert-butanol (2ml) and THF (1.6, ml).

Example 26

3-Butyl-l-ethyl-4-[f2'-(IH-tetrazol-5-yl)-[!,!'-biphenyl3 -4-yl]- methyl]-lH-pyrazole-5-methanol

A solution of Intermediate 22a (1.31g) in absolute ethanol (20ml) was hydrogenolysed over palladium catalyst (0.4g, 5% on activated charcoal) over a period of 72h. A further portion of palladium catalyst (5%;0.4g) and glacial acetic acid (1 ml) was added and the mixture hydrogenolysed for a further 12h. A further portion of palladium catalyst (5%;0.4g) was added with concentrated HCl (1ml) and the mixture hydrogenolysed for a further 12h. The catalyst was filtered off and the filtrate evaporated in vacuo to give a pale yellow foam which was purified by column chromatography on silica gel eluting with System F (100:1). The combined fractions were evaporated and azeotroped with heptane to give the title compound as a colourless foam (0.54g) T.l.c. System F (100:1) Rf 0.2. n.m.r. δ (250 MHz; CDCI3) 0.82 (3H,t), 1.18-1.30 (5H, t +m) , 1.45 (2H,m), 2.40(2H,t), 3.78 (2H,s), 3.98 (2H,q), 4.45 (2H,s), 7.0 (2H, 1/2 A'BB'), 7.1, (2H, 1/2 A'BB'), 7.4 (IH, br.d) , 7.45-7.52 (12H, m), 7.88 (lH,br.d) .

Similarly prepared:—

Example 27

3-Butyl-l- (l-methylethyl) -4- [ [2' - (lH-tetrazol-5-yl) (1, 1' -biphenyl]

- 4 -yl ] methyl ] -lH-pyra zole-5-methanol m.p. 107-118°C

T.l.c. ether:acetic acid (100:1) Rf 0.5

From Intermediate 23a.

Example 28

5-Butyl-l- (1-methylethyl) -4- [ [2' - (IH-tet razol-5-yl) [ 1 , l' -biphenyl]

-4-yl]methyl3 -lH-pyrazole-3 -methanol m.p . 80-84°C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.1

From Intermediate 23b.

Example 29

3-Butyl-l-(2-methylpropyl)-4-[ \2' -(lH-tetrazol-5-yl) [1,1'-biphenyl3

-4-yl3methyl3-lH-pyrazole-5-methanol m.p. 86-89°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.45

From Intermediate 24a.

Example 30

3-Butyl-l- (2-cyclopropylmethyl) -4- [ [2' - (lH-tetrazol-5-yl) [1,1'- bipheny!3 -4-yl3methyl3 -lH-pyrazole-5-methanol m.p. 87-98°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.5

From Intermediate 25a.

Example 31

5-Butyl-l-ethyl-4- [ [2'- ( IH-tet razol-5-yl ) [1,1' -biphenyl] -4- yl 1 methyl] -lH-pyrazole-3-methanol m.p. 103-109°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.5

From Intermediate 22b.

Example 32

3-Butyl-l-cyclobutyl-4-[ [2' - (lH-tetrazol-5-yl) [1,1' -biphenyl]-4- yl]methyl]-lH-pyrazole-5-methanol

T.l.c. System F (95:5) Rf 0.47 n.m.r (250MHz; CDCI3) δ 0.65 (3H,t), 0.95-2.70 (12H,m), 3.58 (2H,s),

4.28 (2H,s), 4.71 (lH,pent), 6.80-7.55 (8H,m) .

From Intermediate 26a.

Example 33

5-Butyl-l-cyclobutyl-4-[ [2' - ( IH-tet razol-5-yl) [1, l'-biphenyl] -4- yl] ethyl] -lH-pyrazole-5-methanol m.p. 85-90°C

T.l.c. dichloromethane:ether:acetic:acid (70:10:1) Rf 0.16

From Intermediate 26b.

Example 34

3-Butyl-l-methyl-4-[ [2 ^r - (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl] methyl] -lH-pyrazole-5-methanol m.p. 125°C

T.l.c. dichloromethane:methanol:acetic acid (200: 15:1) Rf 0.33

From Intermediate 27a.

Example 35

5-Butyl-l-methyl-4- [ [2'- ( IH-tet razo 1-5 -yl) [1, 1' -biphenyl] -4- yl] methyl] -lH-pyrazole-3-methanol m.p. 129°C

T.l.c. dichloromethane .-methanol (9:1) Rf 0.60

From Intermediate 27b.

Example 36 l,3-Dibutyl-4-[ [2'-(lH-tetrazol-5-yl) [1, 1'-biphenyl]-4-yl]methyl]-

IH-pyrazole-5-me hano1 m.p. 125°C

T.l.c. ether:hexane:acetic acid (60:30:1) Rf 0.13

From Intermediate 29a.

Example 37 l,5-Dibutyl-4-[ [2' -(lH-tetrazol-5-yl) [1, l'-biphenvi] -4-yl]methyl] - lH-pyrazole-3-methanol m.p. 56-58°C

T.l.c. System G (60:30:1) Rf 0.17

From Intermediate 29b.

Example 38 l-Ethyl-3-propyl-4-[ [2' - (IH-tet razo 1-5 -yl) [1,1' -biphenyl] -4- yl] methyl] -lH-ρyrazole-5-methanol m.p. 77-82°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.34

From Intermediate 30a.

Example 39 l-Ethyl-5-propyl-4- [ [2' - (lH-tetrazol-5-yl) [1,1' -biphenyl] -4- yl]methyl]-lH-ρyrazole-3-methanol m.p. 69-73°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.45 From Intermediate 30b.

Example 40

1-(1-Methylethyl) -3-propyl-4-[ [2'-(lH-tetrazol-5-yl) fl, 1' -biphenyl]

-4-yl3methyl]-lH-pyrazole-5-methanol m.p. 89-91°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.55

From Intermediate 31a.

Example 41

1-(1-Methylethyl) -5-propyl-4-[ [2'-(lH-tetrazol-5-yl) [1, 1'-biphenyl]

-4-yl]methyl]-lH-pyrazole-3-methanol m.p. 86-90°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.80

From Intermediate 3lb.

Example 42

3-Butyl-l -ethyl- 4- [ [2'- ( IH-tet razol -5- yl ) [1,1' -biphenyl] -4- yl ] methyl 3 -lH-pyrazole-5-carboxaldehyde

Tetra-n-propylammonium perruthenate (TPAP,22mg) was added to a mixture of the product of Example 26 (531mg) , 4-methyl morpholine-N- oxide (224mg) and powdered 4A molecular sieves (6.3g) in a mixture of dry dichloromethane (15ml) and acetonitrile (15ml) at room temperature under nitrogen. The mixture was stirred for lh at room temperature. Further portions of TPAP (27mg) , and 4-methyl morpholine-N-oxide (224mg) were added and the mixture stirred at room temperature for 15 mins. The solvent was evaporated and the residue purified by column chromatography on silica gel eluting with dichloromethane/ether/acetic acid (75:25:1) to give the title compound as a pale purple foam (22 Img) .

T.l.c. dichloromethane/ether/acetic acid (75:25:1) Rf 0.6 n.m.r (250MHz; CDCI3) δ 0.90 (3H,t), 1.25-1.45 (5H, t+m) 1.55 (2H, m) , 2.55 (2H,t), 4.11 (2H,s), 4.49 (2H,q),7.15 (lH,dd), 7.51-7.63 (2H,2xddd), 8.15 (lH,dd), 9.85 (lH,s).

Similarly prepared:-

Example 43

3-Butyl-l-(l-methylethyl) -4-[[2'-(lH-tetrazol-5-yl) [1, 1'-biphenyl]- 4-yl]methyl]-lH-pyrazole-5-carboxaldehyde

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.75 n.m.r. (250MHz, CDCI3) δ 0.9 (3H,t), 1.30 (4H,m) , 1.50 (6H,d), 2.60 (2H,t), 4.10 (2H,s), 5.30 (IH,sept) , 7.20 (4H,A'BB'), 7.4 (lH,br.d), 7.50-7.65 (2H,m), 8.25 (IH, br.d), 9.85 (lH,s). From the product of Example 27.

Example 44

5-Butyl-l-(l-methylethyl)-4-[[2'-(lH-tetrazol-5-yl) [1, 1'-biphenyl}-

4-yl]methyl]-lH-pyrazole-3-carboxaldehvde m.p. 146-148°C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.5

From the product of Example 28.

Example 45

3-Butyl-l-(2-methylpropyl)-4-[[2'-(IH-tetrazol-5-yl) [1,1'-biphenyl]-

4-yl]methyl]-lH-pyrazole-5-carboxaldehyde

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf. 0.45 n.m.r. (250MHz, CDCI3) δ 0.85-0.93 (9H, t+d) , 1.33 (2H,m) , 1.55

(2H,m), 2.15 (lH,sept), 2.57 (2H,t), 4.13 (2H,s) , 4.27 (2H,d), 7.18

(4H, A'BB'), 7.4 (lH,dd), 7.52-7.62 (2H, ) , 8.20 (lH,dd), 9.85

(1H,S) .

From the product of Example 29.

Example 46

3-Butyl-l- (2-cyclopropylmethyl) -4- [ [2 ' - ( lH-tetrazol-5-yl) [ 1 , 1 ' - biphenyl] -4-yl] methyl] -lH-pyrazole-5-carboxaldehvde m.p . 58-60°C

T.l.c. dichloromethane: ther:acetic acid (75:25:1) Rf 0.65

From the product of Example 30.

Example 47

3-Butyl-l-cvclobutyl-4-[ [2'-(lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxaldehvde

T.l.c. System F (95:5) Rf 0.63

IR (CHBr ₃ ) 1677cm ^"1

From the product of Example 32.

Example 48

1, 3-Dibutyl-4-[ [2'-(lH-tetrazol-5-yl) [1, l'-biphenyl]-4-yl]methyl]- lH-pyrazole-5-carboxaldehyde m.p.50-53°C

T.l.c. dichloromethane:ether.-acetic acid (75:25:1) Rf 0.71

From the product of Example 36.

Example 49

1-(1-Methylethyl)-3-propyl-4-[ [2'-(lH-tetrazol-5-yl) [1,1'-biphenyl3-

4-yl]methyl]-lH-pyrazole-5-carboxaldehyde m.p. 48-50°C

T.l.c. dichloromethane.-ether:acetic acid (80:20:1) Rf 0.73

From the product of Example 40.

Example 50

3-Butyl-l-ethyl-4- [ [2' - ( IH-tet razol-5-yl) [1,1' -biphenyl 3 -4- v!3methyl3 -lH-pyrazole-5-carboxylic acid

A solution of sodium chlorite (80%;0.44g) and sodium dihydrogen phosphate (0.44g) in water (5ml) was added to a mixture of the product of Example 27 (203mg) , 2-methyl-2-butene <2M, 0.62ml) and t- butanol (4ml) in THF (10ml) at room temperature. The mixture was stirred vigorously for 20 mins. The mixture was partitioned between ethyl acetate (3x15ml) and water (15ml) . The combined organic extracts were dried. The solvent was evaporated to give a colourless foam (0.2g) which was purified by column chromatography on silica gel eluting with System G (50:50:1) to give the title compound as a colourless solid (60mg) .

T.l.c. System G (50:50:1) Rf 0.25;

Assay Found: C, 66.8; H,6.0; N, 19.4;

^C 24 ^H 26 ^N 6°2 requires: C,67.0; H,6.1; N,19.5%

Similarly prepared: -

Example 51

3-Butyl-l -(1-methylethyl) -4- [ \2' - ( IH-tet razol-5-yl) [1, 1' -biphenyl] -

•_-yl]methyl] -lH-pyrazole-5-carboxylic acid

m.p. 80-81°C

T.l.c. dichloromethane.-ether:acetic acid (120:10:1) Rf 0.2

From the product of Example 43.

Example 52

5-Butyl-l-fl-methviethyl)-4-[ [2'-(lH-tetrazol-5-yl) [1, 1'-biphenyl]

4—yl]methyl]—lH-pyrazole-3-carboxylic acid m.p. 105-108°C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.25

From the product of Example 44.

Example 53

3-Butyl-l-(2-methylpropyl)-4-[ [2'-(lH-tetrazol-5-vD [1, 1'-biphenyl]

4-yl]methyl]-lH-pyrazole-5-carboxylic acid m.p. 178-182 ^C C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.5

From the product of Example 45.

Example 54

3-Butyl-l-cvclobutyl-4-[[2'-(lH-tetrazol-5-yl) [1, l'-biphenyl] -4- yl] methyl] -lH-pyrazole-5-carboxylic acid m.p. 103-106°C

T.l.c. dichloromethane:ether:acetic acid (175:10:1) Rf 0.13

From the product of Example 47.

Example 55

5-[4'-[ r3-Butyl-5- (methoxymethyl) -l-phenyl-lH-pyrazol-4- yl]methyl] [1, 1' -biphenyl] -2-yl ] -lH-tetrazole

Phenylhydrazine and Intermediate 5 were reacted according to the method of Intermediate 18 to give the title compound as a pale yellow foam.

T.l.c. ether:petroleum ether:acetic acid (100:100:1) Rf. 0.2 n.m.r. (250MHz, CH3OD) δ 0.88 (3H,t), 1.30 (2H,m) , 1.48 (2H,m) , 2.52

(2H,t), 3.28 (3H,s), 3.92 (2H,S), 4.30 (2H,s), 7.1 (4H,dd), 7.4-7.7

(9H,m) .

Example 56

l , 3-Diethyl-4- [ [2' - (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4-yl]methyl] lH-pyrazole-5-methanol

From Intermediate 35a according to the method of Example 26.

T.l.c. dichloromethane:methanol (10:1) Rf 0.56

IR (Nujol mull) 3350 & 1494 cm ^"1 .

Example 57

1, 5-Diethyl-4-[ [2'- (lH-tetrazol-5-yl) [1, 1'-biphenyl]-4-yl]methyl] lH-pyrazole-3-methanol

From Intermediate 35b according to the method of Example 26.

T.l.c. dichloromethane:methanol (10:1) Rf 0.36

IR (Nujol mull) 3317 & 1494 cm ^"1 .

Example 58

3-Butyl-4- [ (2 ' -carboxy [ 1 , 1 ' -biphenyl ] -4-yl) methyl 3 -1-ethyl-lH- pyrαzole-5-carboxylic acid

From Intermediate 44 according to the method of Example 4. m.p. 190-192°C

T.l.c. ether:hexane:acetic acid (50:50:1) Rf 0.35

Example 59

3-Butyl-l-propyl-4- [[2'-(lH-tetrazol-5-yl) [1,1' -biphenyl] -4- yl]methyl3-lH-pyrazole-5-methanol

From Intermediate 28a according to the method of Example 26. m.p. 79-82°C.

T.l.c. dichloromethane:methanol (10:1) Rf 0.45

Example 60 l -Ethyl-3 -propyl -4- [ [ 2 ' - ( lH-tetrazol-5-yl ) [ 1 , 1 ' -biphenyl ] - 4- yl 1 methyl] -lH-Pyrazole-5-carboxaldehvde

From the product of Example 38 according to the method of Example

42. m.p . 48-50°:-

T.l ^' .c. dich-oromethane:ether:acetic acid (80:20:1) Rf 0.73.

Example 61 l-Ethyl-3-oroov1-4- [ f2'-(lH-tetrazol-5-yl) [1, 1 ' -biphenyl -4- yl .methyl]-lH-Pyrazole-5-carboxylic acid

From the product of Example 60 according to the method of Example

50. m.p. 118-123°C.

T.l.c. dichloromethane:ether:acetic acid (80:20:1) Rf 0.45.

Example 62 l-(l-Methylethyl)-3-prooyl-4-[ [2'-(lH-tetrazol-5-yl) [1.l'-biphenyl]-

4-yl]methyl]-lH-pyrazole-5-carboxylic acid

From the product of Example 49 according to the method of Example

50. m.p. 108-111°C.

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.62.

Example 63

1,3-Dibutyl-4-[ [2' ^* - (IH-tetrazol-5-yl) [1,1'-biphenyl]-4-yl]methyl]- lH-pyrazole-5-carboxylic acid

From the product of Example 48 according to the method of Example

50. m.p. 185-188°C.

T.l.c. dichloromethane:ether:acetic acid (100:5:1) Rf 0.42.

Example 64

3-Bu yl-4- [ (2' -carbo y [1, 1' -biphenyl] -4-yl) methyl] -1- (1- methylethyl) -lH-Pyrazole-5-carboxylic acid

From the product of Intermediate 46 according to the method of

Example 4. m.p. 213-215°C.

T.l.c. System G (20:20:1) Rf 0.44

The compounds of the invention are tested in vitro for angiotensin II antagonism. Aortic strips are obtained from male New Zealand white rabbits and prepared for recording isometric contractions in response to cumulative addition of angiotensin II. The potencies of test antagonists are assessed by measuring their abilities to displace the angiotensin II cumulative concentration response curve. The method used is that of Ackerly et al . , Proc . Natl. Acad. Sci., 7_4(I2), pp5725-28 (1977) with the exception that

the final composition of the physiological salt solution is as give below in Table 1:

The tissues are initially challenged with K ⁺ <80mM) and the washed at 0, 5, 10 and 15 minutes after the response to K ⁺ ha plateaued. After a further 45 minutes an angiotensin II cumulativ response curve is constructed (O.lnM to O.lμM in 10-fold increments) and the tissues are washed as before. A second, third and fourt angiotensin II cumulative response curve (O.lnM to O.lμM in 3-fol increments) is then constructed at hourly intervals (15 minute washing after each curve followed by 45 minutes equilibration) . Th compounds of the invention (30μM) are tested for angiotensin I antagonism by application 45 minutes before construction of th fourth angiotensin II curve. The third and fourth angiotensin II curves are expressed graphically and a concentration ratio (CR) is calculated by dividing the angiotensin II EC ₅ g value obtained in th presence of the test antagonist (i.e. fourth curve) by the angiotensin II ECC _Q value obtained in the absence of the test antagonist (i.e. third curve).

The potency of the test antagonist is expressed as a pKb which is calculated from the equation :

which is a rearrangement of equation 4 described by Furchgott, in Handbook of Exp. Pharmacol., 33, ρ290 (1972) (eds. Blaschkott and Muscholl) . . -

Compounds of the invention will desirably exhibit a pKb in the range between 5 and 12. Thus we have found that the compounds of the invention inhibit the action of the hormone angiotensin II and are therefore useful in the treatment of conditions in which it is desirable to inhibit angiotensin II activity. In particular, the compounds of Examples are active in the above test.

There is thus provided as a further aspect of the invention a compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof for use in the treatment of conditions associated with excessive or unregulated angiotensin II activity.

In a further or alternative aspect of the invention there is provided a compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof for the manufacture of a therapeutic agent for the treatment of conditions associated with excessive or unregulated angiotensin II activity.

There is also provided in a further or alternative aspect of the invention a method for the treatment of conditions associated with excessive or unregulated angiotensin II activity in a mammal including man comprising administration of an effective amount to a mammal in need of such treatment a compound of general formula (I) or a physiologically acceptable salt, solvate or metabolically labile ester thereof.

The following examples illustrate pharmaceutical formulations according to the invention. The term "active ingredient" is used herein to represent a compound of formula (I) .

Pharmaceutical Example 1

Oral Tablet A

Active Ingredient 700mg

Sodium starch glycollate lOmg

Microcrystalline cellulose 50mg Magnesium stearate 4mg

Sieve the active ingredient and microcrystalline cellulos through a 40 mesh screen and blend in a appropriate blender . Siev the sodium starch glycollate and magnesium stearate through a 6 mesh screen, add to the powder blend and blend until homogeneous Compress with appropriate punches in an automatic tablet press . Th tablets may be coated with a thin polymer coat applied by the fil coating techniques well known to those skilled in the art . Pigment may be incorporated in the film coat .

Pharmaceutical Example 2

Oral Tablet B

Active Ingredient 500mg

Lactose lOOmg

Maize Starch 50mg

Polyvinyl pyrrolidone 3mg

Sodium starch glycollate lOmg

Magnesium stearate 4mg

Tablet Weight 667mg

Sieve the active ingredient, lactose and maize starch through a 40 mesh screen and blend the powders in a suitable blender. Make an aqueous solution of the polyvinyl pyrrolidone (5 - 10% w/v) . Add this solution to the blended powders and mix until granulatad; pass the granulate through a 12 mesh screen and dry the granules in a suitable oven or fluid bed dryer. Sieve the remaining components through a 60 mesh screen and blend them with the dried granules. Compress, using appropriate punches, on an automatic tablet press.

The tablets may be coated with a thin polymer coat applied by film coating techniques well known to those skilled in art . Pigments may be incorporated in the film coat .

Pharmaceutical Example 3

Inhalation Cartridσe

Active Ingredient Img

Lactose 24mg

Blend active ingredient, particle size reduced to a very fine particle size (weight mean diameter ca_. 5μm) with the lactose in a suitable powder blender and fill the powder blender into No. 3 hard gelatin capsules .

The contents of the cartridges may be administered using a powder inhaler.

Pharmaceutical Example 4

Injection Formulation

% w/v Active ingredient 1. 00

Water for injections B .P . to 100.00

Sodium chloride may be added to adjust the tonicity of the solution and the pH may be adjusted to that of maximum stability and/or to facilitate solution of the active ingredient using dilute acid o r alkali or by the addition of suitable buf fer salts . Antioxidants and metal chelating salts may also be included.

The solution is prepared, clarified and filled into appropriate sized ampoules sealed by fusion of the glass . The injection is sterilised by heating in an autoclave using one of the acceptable cycles - Alternatively the solution may be sterilised by filtration and filled into sterile ampoules under aseptic conditions . The solution may be packed under an inert atmosphere of nitrogen .

The present invention is further illustrated by the followin Examples:-

Intermediates 47a and b

( a ) 5- [4 ' - [ [l- (2 , 2-Dimethylpropyl) -3-propyl-5

[ (phenylmethoxy) ethyl 3 -lH-pyrazol-4-yl3 ethyl] [1,1' -biphenyl] -2 yl] -2- (triphenylmethyl) -2H-tetrazole; and

( ) 5 - [ 4 ' - [ [ l- (2 , 2-Dimethylpropyl) -5-ρropyl-3 f (phenylmethoxy)methyl]-lH-pyrazol-4-yl]methyl] [1,1'-biphenyl]-2-yl

2-(triphenylmethyl)-2H-tetrazole

From Intermediate 21 and 2,2-dimethylpropyl iodide according to th method of Intermediate 22.

Intermediate 47a: T.l.c. System A (1:2) Rf 0.29

Intermediate 47b: T.l.c. System A (1:2) Rf 0.21

Intermediates 48a and b

(a) 5-[4'- [ [3-Butyl-5-methoxymethyl-l-(prop-2-enyl) -lH-pyrazol-4 vijmethyl] [1,1'-biphenyl] -2-yl]-2- (triphenylmethyl) -2H-tetrazole; and

(b) 5-[4'- [ [5-Butyl-3-methoxymethyl-l- (prop-2-enyl) -lH-pyrazol-4 yl]methyl] [1, 1'-biphenyl]-2-yl]-2-(triphenylmethyl) -2H-tetrazole

A solution of Intermediate 9 (2.5g) in DMF (10ml) was added dropwis to a suspension of sodium hydride (60% dispersion in oil, 0.22g) i DMF (5ml) at 0"C under nitrogen. 3-Bromopropene (0.46g) was adde to the stirred mixture and stirring continued at room temperatur for 3 hours. The solvent was removed in vacuo and the residu purified by column chromatography eluting with System A (4:3) t give the title compound:

(a) Intermediate 48a as a white foam (860mg), T.l.c. System A (2:1) Rf 0.50

(b) Intermediate 48b as a white foam (lOlmg), T.l.c. System A (2:1) Rf 0.25

Intermediates 49a and b

( a ) 1, 1-Dimethylethyl 4' - [ f 3 -butyl -1 -methyl- 5- [ (phenylmethoxy) methyl] -lH-pyrazol-4-yl]methyl] [1,1' -biphenyl] -2-carboxylate; and

Co) 1 , 1-Dimethylethyl 4 ' - [ [ 5-butyl-l-methyl-3- [ (phenylmethoxy) methyl] -lH-ρyrazol-4-yl]methyl] [1,1' -biphenyl] -2-carboxylate

From Intermediate 37 and methyl iodide according to the method of

Intermediate 22.

Intermediate 49a: T.l.c. System A (1:1) Rf 0.15

Intermediate 49b: T.l.c. System A (1:1) Rf 0.10

Intermediate 50

1, 1-Dimethylethyl 4'-[ [3-butyl-5-(hydroxymethyl) -1-methyl-lH- pyrazol-4-yl]methyl] [1,1'-biphenyl]-2-carboxylate

From Intermediate 49a according to the method of Example 26.

T.l.c. System D (1:1) Rf 0.1

Intermediate 51

1, l-Dimethylethyl 4'- [ [3-butyl-5-formyl-l-methyl-lH-pyrazol-4- yl]methyl] [1,l'-biphenyl]-2-carboxylate

From Intermediate 50 according to the method of Example 42.

T.l.c. System D (1:1) Rf 0.3

Intermediate 52

3-Butyl-4- [ [2' - [ (1 , 1-dimethylethoxy) carbonyl] [ 1, 1 ' -biphenyl] -4- yl]methyl] -l-methyl-lH-pyrazole-5-carboxylic acid

From Intermediate 51 according to the method of Example 50 . m.p . 117-118°C.

Intermediate 53

3-f (2'-Nitro[l, l'-biphenyl]-4-yl)methyl]-!- (phenylmethoxy) -2,4- heptandione

From Intermediate 19 and 4' -(bromomethyl) -2-nitro-l,1'-biphenyl according to the method of Intermediate 17.

T.l.c. System A (1:1) Rf 0.6

Intermediate 54

3-[ (2'-Nitro [1, 1'-biphenyl]-4-yl)methyl]-1-(phenylmethoxy) -2, 4- octandione

From Intermediate 16 and 4'- (bromomethyl) -2-nitro-l, l'-biphenyl according to the method of Intermediate 17.

T.l.c. System A (1:5) Rf 0.4

Intermediate 55

4-f (2'-Nitro[l, 1' -biphenyl] -4-yl) methyl] -5- [ (phenylmethoxy) methyl 1

3-proρyl-lH-pyrazole

From Intermediate 53 and hydrazine hydrate according to the metho of Intermediate 18.

T.l.c. System B (300:8:1) Rf 0.28

Intermediate 56

3-Butyl-4-[ (2' -nit ro [1,1' -biphenyl] -4-yl) methyl] -5- [ (phenylmethoxy) methyl] -lH-pyrazole

From Intermediate 54 and hydrazine hydrate according to the metho of Intermediate 18.

T.l.c. System B (400:8:1) Rf 0.5

Intermediates 57a and b

(a) l-Ethyl-4- [ (2' -nitrofl, l'-biphenyl] -4-yl) methyl] -5- [ (phenylmethoxy) methyl] -3-propyl-lH-pyrazole; and

(b) l-Ethyl-4- [ (2' -ni ro[l, l'-biphenyl ] -4-yl) methyl] -3- [ (phenylmethoxy) methyl] -5-propyl-lH-pyrazole

From Intermediate 55 and ethyl iodide according to the method of

Intermediate 22.

Intermediate 57a: T.l.c. System A (1:1) Rf 0.2

Intermediate 57b: T.l.c. System A (1:1) Rf 0.12

Intermediates 58a and b

(a) 3-Butyl-l-ethyl-4- [ (2' -nitro [1, 1' -biphenyl] -4-yl) methyl] -5- [ (phenylmethoxy) ethyl ]-lH-pyrazole; and

(b) 5-Butyl-l-ethyl-4- [ (2' -nitro [1, 1' -biphenyl] -4-yl)methyl] -3- [ (phenylmethoxy) methyl] -lH-pyrazole

From Intermediate 56 and ethyl iodide according to the method of

Intermediate 22.

Intermediate 58a: T.l.c. System A (1:1) Rf 0.22

Intermediate 58b: T.l.c. System A (1:1) Rf 0.11

Intermediate 59

4'-[[l-Ethyl-5-[ (phenylmethoxy) methyl] -3 -prop yl-lH-pyrazol-4- yl ] methyl ] [1,1' -biphenyl ] -2 -amine

Titanium trichloride solution (15%w/v, 33ml) was added to a solution of Intermediate 57a (1.5g) in acetone (30ml) and the resulting

mixture stirred overnight. Further titanium trichloride solutio (10ml) was added and the reaction warmed to 40 ^* C for 16h. 2N Sodiu carbonate (200ml) was added and the mixture extracted wit dichloromethane (3x150ml) . The combined, dried, organic extract were evaporated in vacuo and the residue purified by chromatograph eluting with System A (2:1) to give the title compound as a yello coloured oil (1.03g). T.l.-c. System A (4:1), Rf 0.36

Similarly prepared:-

Intermediate 60

4'- [ [3-Butyl-l-ethyl-5-[ (phenylmethoxy)methyl] -lH-pyrazol-4- yl]methyl] [1,1'-biphenyl]-2-amine

T.l.c. System A (2:1) Rf 0.28

From Intermediate 58a.

Intermediate 61

N-[4'-[ [l-Ethyl-5- [ (phenylmethoxy) ethyl]-3-propyl-lH-pyrazol-4- yl]methyl] [1, 1'-biphenyl]-2-yl] rifluoromethanesulphonamide A solution of Intermediate 59 (Ig) and triethylamine (0.35ml) in dry dichloromethane (20ml) at -70°C was treated dropwise with a solution of triflie anhydride (0.46ml) in dichloromethane (5ml) and the resulting mixture stirred at -70 C for lh. Water (10ml) was added and the reaction allowed to warm to room temperature. The aqueous layer was separated and the organic washed with 2N hydrochloric acid (10ml) . The dried organic solution was evaporated in vacuo and the residue purified by chromatography eluting with System B (300:8:1) to give the title compound as a foam (0.93g) . T.l.c. System B (100:8:1), Rf 0.31

Similarly prepared:-

Intermediate 62

K-{ * -[ [3-Butyl-l-ethyl-5-[ (phenylmethoxy)methyl] -lH-pyrazol-4- ylϊmethyi] [1, 1'-biphenyl]-2-yl]trifluoromethane-sulphonamide

T.l.c. System B (150:8:1) Rf 0.15

From Intermediate 60.

Examples 65 to 71 inclusive were prepared according the metho of Example 26:-

Example 65

5-Butyl-l- (2-methylpropyl) -4- [ [2' - (lH-tetrazol-5-yl) [1, 1' -biphenyl]

4-yl] methyl] -lH-pyrazole-3-methanol m.p. 65-68°C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.15

From Intermediate 24b.

Example 66

5-Butyl-l- (2-cvclopropylmethyl) -4-[ [2' - (lH-tetrazol-5-yl) [1, 1' biphenyl] -4-yl] methyl] -lH-pyrazole-3-methanol m.p. 70-75°C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.15

From Intermediate 25b.

Example 67

5-Butyl-l -propyl -4- [ [2 ' - ( lH-tetrazol-5-yl) [1,1' -biphenyl] -4- yl] ethyl] -lH-pyrazole-3-methanol m.p. 59-62°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.41

From Intermediate 28b.

Example 68

1- (2-Methylpropyl) -3-propyl-4- [ [2'- (IH-tetrazol-5-yl) f1, l'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-methanol m.p. 104-108°C

T.l.c. dichloromethane:methanol (10:1) Rf 0.7 From Intermediate 45a.

Example 69

1- (2-Methylpropyl) -5-propyl-4- f [2'-(lH-tetrazol-5-yl) [1, l'- biphenyl] -4-yl]methyl] -lH-pyrazole-3-methanol rr,.p. 55-61°C

T.l.c. dichloromethane :methanol (10:1) Rf 0.57 From Intermediate 45b.

Example 70 l- (2, 2-Dimethylpropyl) -3-propyl-4- [ [2 ' - (lH-tetrazol-5-yl) [1, 1' - biphenyl]-4-yl]methyl]-lH-pyrazole-5-methanol m.p. 79-80°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.3

From Intermediate 47a.

Example 71 l-(2,2-Dimethylpropyl)-5-propyl-4-[ [2'-(lH-tetrazol-5-yl) [1,1'- biphenyl]-4-yl]methyl]-lH-pyrazole-3-methanol m.p. 136-139°C

T. l.c. dichloromethane: ether: acetic acid (90 :10 : 1) Rf 0.28

From Intermediate 47b.

Examples 72 to 75 inclusive were prepared according to the method of Example 42 :-

Examole 72

3-Butyl-l-methyl-4- [ [2 ' - (lH-tetrazol-5-yl ) [ 1 , 1 ' -biphenyl] -4- yl] methyl] -lH-pyrazole-5-carboxaldehvde m.p. 53-56°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.3

From the product of Example 34.

Example 73

3-Butyl-l-propyl-4-[ [2' - (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxaldehyde m.p. 48-50°C

T.l.c. dichloromethane:ether:acetic acid (75:25:1) Rf 0.68

From the product of Example 59.

Example 74

1 - (2-Methylpropyl ) -3 -propyl- 4- [ [2 ' - ( IH-tet ra zo 1-5 - yl ) [ 1 , 1 ' - biphenyl] -4 -yl] methyl ] -lH-pyrazole-5-carboxaldehyde m.p. 54-56°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.57

From the product of Example 68.

Example 75

1- (2,2-Dimethylpropyl) -3-propyl-4- [ [2'- ( IH-tet razol-5-yl) [1,1'- biphenyl] -4-yl]methyl] -lH-pyrazole-5-carboxaldehyde m.p. 60-62°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.46

From the product of Example 70.

Examples 76 to 80 inclusive were prepared according to the method of Example 50:-

Ξxample 76

3-Butyl-l- (2-cvclopropylmethyl) -4- [ [2' - (IH-tetrazol-5-yl) [1, l'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid m.p. 205-206°C

T.l.c. dichloromethane:ether:acetic acid (72:25:1) Rf 0.5 From the product of Example 46.

Example 77

3-Butyl-l-methyl-4- [ [2'- (lH-tetrazol-5-yl) [1, 1' -biphenyl] -4- yl]methyl]-lH-pyrazole-5-carboxylic acid m.p. 118-122°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.23

From the product of Example 72.

Example 78

3-Butyl-l-propyl-4- [ [2' - (lH-tetrazol-5-yl) [1,1' -biphenyl] -4- yl]mβthyl]-lH-pyrazole-5-carboxylic acid m.p. 181-183°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.36

From the product of Example 73.

Example 79

1- (2-Methylpropyl) -3 -prooyl-4-[ [2'- (lH-tetrazol-5-yl) [ 1, l'- biphenyl] -4-yl]methyl] -lH-pyrazole-5-carboxylic acid m.p. 138-140°C

T.l.c. dichloromethane:ether:acetic acid (90:10:1) Rf 0.22 From the product of Example 7 .

Example 80 l-(2,2-Dimethylpropyl) -3-p_ropyl-4- f f2' - (lH-tetrazol-5-yl) fl, 1'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid m.p. 122-126°C

T.l.c. dichloromethane:ether:acetic acid (95:5:1) Rf 0.3

From the product of Example 75.

Example 81

5- [4' - [ [5-Butyl-3-methoxymethyl-l- (1, 1-dimethylethyl) -lH-pyrazol-4- yl] methyl] [1, 1' -biphenyl] -2-yl] -lH-tetrazole

A solution of Intermediate 5 (5g) , 1, 1-dimethylethylhydrazine hydrochloride (959mg) and triethylamine (1.11ml) in ethanol (50m) was heated at 60 °c for 6h after which time additional triethylamine (2.22ml) and 1, 1-dimethylethylhydrazine hydrochloride (1.92g) were added and the resultant mixture stirred at 60 "c for a further 18h and then at room temperature for 24h . The solvents were removed in vacuo and the residue purified by column chromatography eluting with petroleum ether: ether (2:1) initially followed by ethanol. The concentrated ethanolic extracts were further purified by column chromatography eluting with System B (100:5:1) to give the title compound (746mg) as a cream coloured foam, mp 58-60 ^* C

Example 82 l,5-Dibutyl-4-[ [2' -( IH-tet razol-5-yl) [1, l'-biphenyl] -4-yl] methyl] - lH-pyrazole-3-carboxylic acid

A solution of potassium permanganate (55mg) in water (3ml) was added to a stirred solution of the product of Example 37 (140mg) in acetone (3ml) at 50"C. The resulting mixture was stirred at 65"C for l ^l /z hours. A further portion of potassium permanganate (55mg) was added and the mixture stirred for 3 hours. Sodium met abi sulphite (5% w/v, 15ml) was added and the aqueous phase was extracted with ethyl acetate (3x30ml) . The combined organic extracts were dried and concentrated to yield a white foam which was purified by column chromatography, eluting with ether/dichloromethane (1:1) to yield the title compound as a white solid (70mg) .

m.p. 108'C

Example 83

5- [ 4' - [ [3-Butyl-5-methoxymethyl-l- (prop-2-enyl) -lH-pyrazol-4- yl] methyl] [1, 1' -biphenyl] -2-yl] -lH-tetrazole

A solution of Intermediate 48a (800mg) , methanol (15ml) and concentrated hydrochloric acid (0.5ml) was stirred at room temperature for 3 hours. The pH of the solution was adjusted to pH9 (2N NaCθ ) and the solvent removed in vacuo. The residue was partitioned between water (20ml) and ether (3x20ml) . The aqueous layer was then acidified to pH3 (2NHC1) and extracted into ethyl acetate (3x20ml) . The ethyl acetate fractions were combined, dried and the solvent removed in vacuo to afford the title compound as a white foam (470mg) . m.p. 39-41'C T.l.c. ether Rf 0.40

Similarly prepared :-

Example 84

5- [ ' - [ [5 -Butyl- 3 -methoxymethyl- 1- (prop-2-enyl ) -lH-pyrazol-4- yl3 methyl 3 [1, l'-biphenyl3-2-yl3-lH-tetrazole as a white foam (600mg) .m.p. 37-40°C T.l.c. ether Rf 0.24

From a solution of Intermediate 48b (l.OOg), methanol (15ml) and concentrated hydrochloric acid (0.5ml).

Example 85

3-Butyl-4- [ (2'carboxyfl, l'-biphenyl] -4-yl)methyl] -1-methyl-1H- pyrazole-5-carboxylic acid

From Intermediate 52 according to the method of Example . m.p. 168-170°C.

Analysis Found C,70.2; H,6.3; N,6.8;

^C 23 ^K 24 ^N 2°4 requires C,70.4; H,6.2; N,7.1%

Example 86

N- f ' - [ [l-Ethyl-5- (hydroxymethyl) -3-proDyl-lH-pyrazol- . yl] methyl] [1,1' -biphenyl] -2-yl] trif luoromethanesulphonamide

A solution of Intermediate 61 (0.89g) in absolute ethanol (25ml) and 2N hydrochloric acid (0.7ml) was hydrogenated with 10% palladium on carbon catalyst (0.6g) for l ¹ /2h. The reaction was filtered into a flask containing 2N sodium carbonate solution (0.7ml) and the solvent removed in va cuo . The residue was taken up in dichloromethane (15ml) and washed with water (15ml) . The organic Ξ -Iution was dried and evaporated in vacuo to give the title compound as a white foam (0.65g) m.p. 59-64"C. T.l.c. System A Rf 0.19

Similarly prepared: -

Example 87

N-[4'-[ [3-Butyl-l-ethyl-5- (hydroxymethyl) -lH-pyrazol-4- yl]methyl] [1, l'-biphenyl] -2-yl] trif luoromethanesulphonamide

T.l.c System B (150:8:1) Rf 0.12

Analysis Found C,57.8; H,6.1: N,8.2;

^C 24 ^H 28 ^N 3°3 requires C,58.2; H,5.7; N,8.5%

From Intermediate 62.

Example 88

N-[4'-[ [l-Ethyl-5-formyl-3-propyl-lH-pyrazol-4-yl]methyl] [1, l'- biphenyl] —2-yl] rif luoromethanesulphonamide

Tetra-n-propylammonium perruthenate (46mg) was added to a mixture of the product of Example 86 (630mg) , N-methylmorpholine N-oxide (0.46g) and 4A molecular sieves (2.5g) in dichloromethane (10ml) and dry acetonitrile (10ml) and the reaction left for 5min. The reaction was filtered, solvent removed in vacuo and the residue adsorbed onto silica. The material was purified by chromatography eluting with System A (4:1) to give the title compound as a yellow gum (0.44g) .

T.l.c. System A (4:1), Rf 0.64 I.r. (CHBr ₃ ) 1679, 1597, 1366 cm ^"1 .

Similarly prepared:-

Ξxample 89

N-[4' -r r3-Butyl-l-ethyl-5-formyl-lH-pyrazol-4-yl]methyl] [1, l'- biphenyl]-2-yl]trifluoromethanesulphonamide T.l.c. System A (3:1) Rf 0.56 n.m.r. (CDCI3, 250MHz) δ 0.94 (3H,t), 1.44 (3H,t), 1.62 (2H,m) , 2.55 (2H,t), 4.15 (2H,s), 4.54 (2H, quad), 7.25-7.45 (7H,m) , 7.64 (lH,m) , 9.9 (lH,s) . From the product of Example 87.

Example 90 l-Ethyl-3-propyl-4- [ [2'-[ [ (trifluoromethyl) sulphonyl]amino] [1,1'- biphenyl]-4-yl]methyl]-lH-pyrazole-5-carboxylic acid A solution of sodium chlorite (0.4g) and sodium dihydrogen orthophosphate (2.56g) in water (5ml) was added to a solution of the product of Example 88 (430mg) and 2-methyl-2-butene (4.5ml, 2M solution in TKF) in t-butanol (5ml) and THF (10ml) and the resulting mixture stirred for 3h. Solvent was removed in vacuo, the residue taken up in dichloromethane (10ml) and washed with water (10ml) . 2N Sodium hydroxide (10ml) was added to the organic solution and the organic layer removed. 2N Hydrochloric acid (-llrnl) was added to the aqueous phase which was then extracted with ethyl acetate (2x15ml) . The dried organic extracts were evaporated in vacuo to give a yellow gummy solid which was recrystallised from System D (1:1) to give the title compound as a white solid (250mg) m.p. 68- 72 ^* C. T.l.c. System A (2:1), Rf 0.53

Similarly prepared:-

Example 91

3-Butyl-i-ethyl-4- [ [2' - [ [ (trifluoromethyl) sulphonyl] amino] [1,1'- biphenyl-4-yl]methyl]-lH-pyrazole-5-carboxylic acid

T.l.c. System G (300:100:4) Rf 0.55 n.m.r. {CDCI3, 250MHz) δ 0.87 (3H,t), 1.32 (2H, m) , 1.44 (3H,t),

1.55 <2H,m), 2.56 (2H,t), 4.18 (2H,s), 4.58 (2H,q), 6.67 (lH,br.s),

7.17-7.3 (7H,m), 7.37 (lH,dt), 7.61 (lH,br.d).

From the product of Example 89.