Title:
CAPTURE AND ANALYSIS OF TARGET GENOMIC REGIONS
Document Type and Number:
WIPO Patent Application WO/2020/232081
Kind Code:
A3
Abstract:
The disclosure pertains to materials and methods for capturing target genomic regions, comprising cleaving the target genomic region using endonucleases at specific recognition sites having a minimum of between about 10 and about 30 nucleotides and capturing the target genomic region by hybridizing to a bridge oligonucleotide. The disclosure also pertains to analyzing the captured target genomic regions. The endonucleases used for cleavage can be programmable endonucleases that specifically bind to the recognition sites to direct the cleavage. The captured target genomic regions can be amplified, preferably, via polymerase chain reaction or rolling circle amplification and detected or sequenced. Further, the invention pertains to kits for performing the methods of the invention, comprising one or more endonucleases designed to cleave one or more target genomic regions and bridge oligonucleotides designed to capture the one or more target genomic regions. The kits can be customized for specific target genomic regions.
Inventors:
NEVES LEANDRO (US)
Application Number:
PCT/US2020/032615
Publication Date:
December 24, 2020
Filing Date:
May 13, 2020
Export Citation:
Assignee:
RAPID GENOMICS LLC (US)
International Classes:
C12N15/00; C12N15/09; C12N15/10; C12N15/11
Foreign References:
| US20130316917A1 | 2013-11-28 | |||
| US20170211099A1 | 2017-07-27 |
Attorney, Agent or Firm:
EISENSCHENK, Frank, C. et al. (US)
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