Title:
CARBONIC ANHYDRASE POLYPEPTIDES AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2011/041011
Kind Code:
A3
Abstract:
The present disclosure relates to recombinant carbonic anhydrase polypeptides having improved properties as compared to a naturally occurring γ-class carbonic anhydrase from Methanosarcina thermophila and uses of the polypeptides for the sequestration of carbon dioxide as well as for the release of carbon dioxide from a composition comprising bicarbonate. Also provided are polynucleotides encoding the recombinant carbonic anhydrase polypeptides and host cells capable of expressing the recombinant carbonic anhydrase polypeptides.
Inventors:
NEWMAN LISA M (US)
CLARK LOUIS (US)
CHING CHARLENE (US)
ZIMMERMAN SABRINA (US)
CLARK LOUIS (US)
CHING CHARLENE (US)
ZIMMERMAN SABRINA (US)
Application Number:
PCT/US2010/042003
Publication Date:
August 04, 2011
Filing Date:
July 14, 2010
Export Citation:
Assignee:
CODEXIS INC (US)
NEWMAN LISA M (US)
CLARK LOUIS (US)
CHING CHARLENE (US)
ZIMMERMAN SABRINA (US)
NEWMAN LISA M (US)
CLARK LOUIS (US)
CHING CHARLENE (US)
ZIMMERMAN SABRINA (US)
International Classes:
C12N9/02; C12N15/53; C12R1/11
Domestic Patent References:
| WO2010081007A2 | 2010-07-15 |
Foreign References:
| US20060257990A1 | 2006-11-16 |
Other References:
IVERSON, T. M. ET AL.: "A closer look at the active site of gamma-class carbo nic anhydrases: high-resolution crystallographic studies of the carbonic anh ydrase from Methanosasrcina thermophila.", BIOCHEMISTRY, vol. 39, no. 31, 8 August 2000 (2000-08-08), pages 9222 - 9231
BOOTORABI, F. ET AL.: "'Modification of carbonic anhydrase II with acetaldehyd e, the first metabolite of ethanol, leads to decreased enzyme activity", BMC BIOCHEMISTRY, vol. 9, 27 November 2008 (2008-11-27), pages 32, XP021041709, DOI: doi:10.1186/1471-2091-9-32
NAGELHUS, E. ET AL.: "Carbonic anhydrase XIV is enriched in specific membrane domains of retinal pigment epithelium, Muller cells, and astrocytes", PNAS, vol. 102, no. 22, 18 May 2005 (2005-05-18), pages 8030 - 8035
ULMASOV, B. ET AL.: "Purification and kinetic analysis of recombinant CA XII, a membrane carbonic anhydrase overexpressed in certain cancers", PNAS, vol. 97, no. 26, 19 December 2000 (2000-12-19), pages 14212 - 14217
MOYA, A. ET AL.: "Carbonic Anhydrase in the Scleractinian Coral Stylophora pi stillata", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 37, 10 July 2008 (2008-07-10), pages 25475 - 25484
BOOTORABI, F. ET AL.: "'Modification of carbonic anhydrase II with acetaldehyd e, the first metabolite of ethanol, leads to decreased enzyme activity", BMC BIOCHEMISTRY, vol. 9, 27 November 2008 (2008-11-27), pages 32, XP021041709, DOI: doi:10.1186/1471-2091-9-32
NAGELHUS, E. ET AL.: "Carbonic anhydrase XIV is enriched in specific membrane domains of retinal pigment epithelium, Muller cells, and astrocytes", PNAS, vol. 102, no. 22, 18 May 2005 (2005-05-18), pages 8030 - 8035
ULMASOV, B. ET AL.: "Purification and kinetic analysis of recombinant CA XII, a membrane carbonic anhydrase overexpressed in certain cancers", PNAS, vol. 97, no. 26, 19 December 2000 (2000-12-19), pages 14212 - 14217
MOYA, A. ET AL.: "Carbonic Anhydrase in the Scleractinian Coral Stylophora pi stillata", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 37, 10 July 2008 (2008-07-10), pages 25475 - 25484
Attorney, Agent or Firm:
WHITING, Adam, K. (INC.200 Penobscot Driv, Redwood City California, US)
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