This invention relates to a method of treatment using an antagonist of the human 11 CBy receptor; a new therapeutic use of a class of carboxamide compounds which are antagonists to a human 1 CBy receptor; also to novel compounds within that class, and to methods for making the compounds.

International Patent Application Publication Number WO 01/21577 (Takeda Chemical Industries Ltd.) discloses certain bisaryl compounds as melanin concentrating hormone antagonists.

WO 98/00401 (Merck & Co. Inc.) discloses benzamid derivatives as fibrinogen receptor antagonist prodrugs.

European Patent EP 0 358 118 (Boehringer Mannheim GmbH) discloses certain bisaryl compounds as inhibitors of erythrocyte aggregation and useful in the treatment of cardiac and circulatory disease.

European Patent Application EP 0 968 999 (Mitsui Chemical Inc.) discloses certain anilide derivatives useful in the treatment of arrhythmia.

WO 99/01127 (SmithKline Beecham) discloses certainN-[(amino alkoxy) phenyl] benzamides that are active as CCR5 receptor ligands, including the compounds N- [4- [2- [bis (l-methylethyl) amino] ethoxy]-2-fluorophenyl]-[1, 1'-biphenyl]-4- carboxamide and N- [4- [2- [bis (l-methylethyl) amino]-ethoxy]-phenyl]- [1, 1'-biphenyl]-4- carboxamide. Also WO 99/06146 (SmithKline Beecham) discloses certain substituted anilides that are antagonists of the CCR5 receptor, including the compounds: biphenyl-4-carboxylic acid [4- (2-dimethylamino-ethoxy)-phenyl]-amide, biphenyl-4-carboxylic acid [4- (2-diisopropylamino-ethoxy)-phenyl]-amide, <BR> <BR> <BR> N [4- (2-diisopropylamino-ethoxy)-phenyl]-4-phenoxy-benzamide,< BR> <BR> <BR> <BR> <BR> N [4- (2-diethylamino-ethoxy)-phenyl]-4-phenoxy-benzamide,<BR&g t; <BR> <BR> <BR> <BR> N-[4- (2-diisopropylamino-ethoxy)-phenyl]-3-phenoxy-benzamide, N-[4-(2-diethylamino-ethoxy)-phenyl]-3-phenoxy-benzamide,

4-cyclohexyl-N-[4- (2-diisopropylamino-ethoxy)-phenyl]-benzamide, 4-cyclohexyl-N- [4- (2-diethylamino-ethoxy)-phenyl]-benzamide, 4-benzyl-N-[4- (2-diisopropylamino-ethoxy)-phenyl]-benzamide, 4-benzyl-N- [4- (2-diethylamino-ethoxy)-phenyl]-benzamide, 4'-ethyl-biphenyl-4-carboxylic acid [4- (2-diisopropylamino-ethoxy)-phenyl]-amide, and 4'-ethyl-biphenyl-4-carboxylic acid [4-(2-diethylamino-ethoxy)-phenyl]-amide.

The present invention is based on the finding that a class of carboxamides overlapping with the above-mentioned benzamides and anilides, are, surprisingly, antagonists of a human 11 CBy receptor disclosed in Nature, 400,261-265 (1999).

Accordingly these compounds are believed to have a role in preventing, ameliorating or correcting dysfunctions or diseases, including, but not limited to, infections such as bacterial, fungal, protozoan and viral infections, particularly infection caused by HIV-1 or HIV-2; pain; cancers; diabetes ; obesity; feeding and drinking abnormalities, such as anorexia and bulimia; asthma; Parkinson's disease; both acute and congestive heart failure; hypotension; hypertension; urinary retention; osteoporosis; angina pectoris; myocardial infarction ; ulcers; allergies; benign prostatic hypertrophy; psychotic and neurological disorders, including anxiety, schizophrenia, manic depression, delirium, dementia or severe mental retardation; and dyskinesias, such as Huntington's disease or Gilles de la Tourette's syndrome, among others, hereinafter referred to as"the Disorders".

According to the present invention there is provided a method of treating the Disorders which comprises administering to a mammal suffering from one or more of the Disorders an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt or solvate thereof, in which : (I)

each A is independently hydrogen, a C1 6 alkyl optionally substituted by hydroxyl, C1 6 alkoxy, C1 6 alkenyl or C1 6 acyl group or a halogen atom or hydroxyl, CN or CF3 group; R3 is hydrogen, methyl or ethyl.

Preferably R3 is methyl.

R4 is an optionally substituted aromatic carbocyclic or heterocyclic ring.

Z is an O or S atom, or an NH or CH2 group, or a single bond, at the 3 or 4 position of R4 relative to the carbonyl group.

Preferably, Z is a bond.

More preferably, Z is a bond at the 4-position of R4 relative to the carbonyl group.

R5 is an optionally substituted aromatic carbocyclic or heterocyclic ring, or an optionally substituted, saturated or unsaturated, carbocyclic or heterocyclic ring.

Preferably, R5 is a phenyl ring.

(a) where X is an O or S atom, preferably an O atom ; Y is a linear or branched C2-4 allcylene group, preferably a C3 alkylene group, optionally substituted by a hydroxyl group, or is a Cs 6 cycloalkylene group, RI and R2 are independently a linear or branched C1 6 alkyl, preferably ethyl; phenyl C 6 alkyl group; or (b) where X is an O or S atom; Y is a linear or branched C2-4 alkylene group, optionally substituted by a hydroxyl group, RI and R2 are linked to form a 5,6 or 7-membered ring, preferably a 5-membered ring, optionally containing one or more further heteroatoms selected from O, S or N, where N or C ring atoms are optionally substituted by Ra,-CO-Ra,-CO-NH-Ra, or CO-O-Ra, where Ra is a linear or branched C1-6 alkyl or aryl group ; and the 5,6 or 7-membered ring is optionally fused to an optionally substituted benzene ring, or a ring atom of the 5, 6 or 7-membered ring is optionally linked by a single bond or methylene group to Y; or (c) where X is an O or S atom,

Y is a C2-4 alkylene group, Rl is a C2-4 alkylene group linked to Y to form a 5 or 6 membered ring and R2 is a linear or branched C1 6 alkyl group; or (d) where X is a N atom, Y is a C2-4 allcylene group, RI is a C2-4 alkylene group linked to X to form a 5 or 6 membered ring and R2 is a linear or branched C1 6 alkyl group.

Alkyl groups, including alkyl groups that are part of alkoxy, acyl, etc groups, typically contain 1 to 6 carbon atoms, and may be linear or branched, such as methyl, ethyl, i- propyl and t-butyl, and optionally substituted by hydroxyl. Aryl groups are typically phenyl, but may include bicyclic groups such as naphthyl. Cycloalkyl groups typically contain from 3 to 7 carbon atoms. Heterocyclic groups may be monocylic 5 to 7 membered rings containing up to three hetero atoms, such as pyridyl or imidazole, or bicyclic, especially heterocyclic rings fused to benzene rings, such as benzoxazole or benzimidazole. Aryl, cycloalkyl and heterocyclic groups may be optionally subsituted by up to three substituents, which may suitably be selected from aryl, alkyl, alkoxy, halogen, hydroxy and cyano, or by linked substituents such as dioxymethylene.

Suitable aromatic rings for use as R4 include phenyl, pyridyl, thienyl, furanyl and pyrazolyl. Suitable optional substituents for R4 include halogen, CF3, C1 4 alkyl, C1 4 alkoxy. R4 may have 2 or 3 substituents, but preferably has only 1 substituent in addition to Z, or more preferably is unsubstituted apart from Z. Particularly suitable substituents for R4 include chloro, fluoro, trifluoromethyl, methyl, methoxy.

R5 may be monocyclic, for example thienyl, furanyl, imidazolyl, oxadiazolyl, phenyl, pyridinyl, cyclohexyl, piperidinyl, piperazinyl, pyrazinyl, pyrimidinyl; or a fused bicyclic ring system, for example naphthyl, 3,4-dioxymethylene-phenyl, benzofuranyl, indolyl; or a bicyclic system in which a monocyclic ring has a cyclic substituent such as oxadiazolyl, benzyloxy. Suitable optional substituents for R5 include halogen, CF3, CF30, CHF20, CN, amino, mono-or di-Cl 6 alkylamino, C1 6 alkyl, C1 6 allcoxy, C1 6 acyl, C1 6 alkyl- S-, C1 6 alkyl-SO2-, C1 6 alkenyl, phenyl-Cl 6 alkyl, phenyl-Cl 6 alkoxy. R5 may have 2 or 3 substituents, but preferably has only 1 substituent, especially in the para position relative to Z. Particularly suitable substituents for R5 include chloro, fluoro,

trifluoromethyl, cyano, amino, methyl, ethyl, t-butyl, methoxy, acetyl, formyl, methylthio, methanesulphonyl, vinyl, benzyl, benzyloxy, hydrogen.

As for the ring substituents A, all A substituents may be hydrogen, but it is advantageous that no more than 3 are hydrogen. Suitable A substituents include halogen, C1_6 alkyl optionally substituted with hydroxy, C 1-6 alkoxy, C1-6 acyl and C1 6 alkenyl.

Particularly suitable A substituents include C1-2alkoxy, C1-2alkyl, C1-2 acyl. Preferable substituents for A include chloro, fluoro, methyl, ethyl, hydroxyethyl, methoxy, formyl, acetyl, vinyl and allyl. More preferable substituents for A include methoxy.

Suitably, the A substituent is adjacent to the group Q.

In the system Q, in configuration (a) particularly suitable substituents for RI and R2 include methyl, ethyl, isopropyl, benzyl, phenethyl. Y may especially be- (CH2) 2-, - (CH2) 3-,- (CH2) 4-,-CH2-CH (CH3)-CH2-. When Y is substituted by hydroxy, it may be for example-CH2-CH (OH)-CH2-.

In configuration (b) of system Q, the ring formed by linking RI and R2 may be pyrrolidinyl, piperidinyl, azepanyl, or imidazolyl. Fused rings include indolinyl, tetrahydroisoquinolinyl, tetrahydroquinolinyl and benzoazepinyl. When a second heteroatom is present, suitable rings include thiazinyl, oxazinyl and piperazinyl. A second N atom may be substituted, for example by phenyl, methyl, ethyl, isopropyl or acetyl. Y is typically- (CH2) 2-. The ring may be linked back to Y to form a quinuclidinyl group.

In configuration (c) of system Q, the ring formed by linking RI to Y may be a pyrrolidinyl or piperidinyl ring. The linkage to Y may be such as to create a ring linked by a single bond from a ring carbon atom directly to X or via a methylene or ethylene linking group. R2 is typically methyl so that the N atom of the ring is substituted by methyl.

In configuration (d) of system Q, the ring formed by linking Rl to N is suitably a 5 or 6- membered ring such as diazinyl or piperazinyl. Y is typically- (CH2) 2-. R2 is typically methyl so that the second N atom (other than X) of the ring is substituted by methyl.

Within the scope of formula (I) is a class of compounds of general formula (II) (II) where A = H and OMe, R3 = H, X = O, Y = CH2CH2, Z = a bond, R4 = Ph, R5 is either meta or para substituted on R4, and RI, R2 and R5 are as defined for formula (I).

Also within the scope of formula (I) is a class of compounds of general formula (III) (III) where A = H and OMe, R3 = H, X = O, Y = CH2-CH2, Z = O, CH2 or NH and is either meta or para substituted on R4, R4 = Ph, R5 is Ph, and Rl and R2 are as defined for formula (I).

Also within the scope of formula (I) is a class of compounds of general formula (IV)

where A = H and OMe, Rl = R2 = iPr, R3 = H, X = O, Y = CH2-CH2, and R4 and R5 are substituted phenyl or heterocycles as defined for formula (I) Also within the scope of formula (I) is a class of compounds of general formula (V) (V) where R3 = H, X = O, Y = CH2-CH2, Z = O2, CH2, NH or a bond, R4 = Ph, R5 is Ph or cyclohexyl (Cy), Z is either meta or para substituted on R4, and A (R6, R7) and R1, R2 are as defined in formula (I).

Also within the scope of formula (I) is a class of compounds of general formula (VI) (VI) where X = O, Y = CH2-CH2, R4 = phenyl, R5 = phenyl or cyclohexyl (Cy), Z = O, CH2 or a bond, and A (R8, R9), R3 and R1, R2 are as defined in formula (I).

Also within the scope of formula (I) is a class of compounds of general formula (VII)

(VII) where A = H and OMe, X = O, R3 = H, R4 = 3-pyridyl (with respect to the carbonyl group), R5 = phenyl, Z = a para bond, and R1, R2 are as defined in formula (I).

Also within the scope of formula (I) is a class of compounds of general formula (VIII) (VIII) where A = H and OMe, R3 = H, X = O, R4 = phenyl, Z = O, CH2 or a bond, R5 = Ph or cyclohexyl (Cy), Y is a chain of 3 or 4 carbon atoms optionally substituted by an hydroxyl group, and R1, R2 are as defined in formula (I).

Also within the scope of formula (I) is a class of compounds of general formula (IX) (IX) where A = H and OMe, R3 = H, X = N, R4 = phenyl, Z = a para substituted bond, R5 = Ph or cyclohexyl (Cy), Y and R2 form a piperazinyl ring between X and N, and Rl is as defined in formula (I).

A preferred sub-class of compounds for use in the method of treatment of this invention are compounds of formula (I) in which R3 is methyl.

Within formula (I) is a novel group of compounds in which R3 is methyl or ethyl. The novel compounds, or a salt or solvate thereof, form a further aspect of this invention.

A particular group of novel compounds is a class of compounds of general formula (VI) where R8 and R9 are as defined for A in formula (I), Rl, R2 and R5 are as defined in formula (I), and R3 is methyl or ethyl.

Suitably R5 is phenyl or cyclohexyl optionally substituted by halogen, haloalkyl, alkyl or alkoxy; Z is O, CH2 or a single bond; R8 and R9 are independently selected from hydrogen, halogen, alkyl and alkoxy; RI and R2 are alkyl or linked together to form a ring; and R3 is ethyl or methyl.

Another aspect of this invention is a class of novel compounds, or a salt or solvate thereof, which are the compounds of formula (I) excluding the compounds: N- [4- [2- [bis (l-methylethyl) amino] ethoxy]-2-fluorophenyl]-[1, 1'-biphenyl]-4- carboxamide, N- [4- [2- [bis (l-methylethyl) amino] ethoxy] phenyl]- [1, 1'-biphenyl]-4-carboxamide, biphenyl-4-carboxylic acid [4- (2-diisopropylamino-ethoxy)-phenyl]-amide, N [4- (2-diisopropylamino-ethoxy)-phenyl]-4-phenoxy-benzamide,< BR> N-[4-(2-diethylamino-ethoxy)-phenyl]-4-phenoxy-benzamide,< ;BR> N [4- (2-diisopropylamino-ethoxy)-phenyl]-3-phenoxy-benzamide, N- [4- (2-diethylamino-ethoxy)-phenyl]-3-phenoxy-benzamide, 4-cyclohexyl-N- [4- (2-diisopropylamino-ethoxy)-phenyl]-benzamide, 4-cyclohexyl-N- [4- (2-diethylamino-ethoxy)-phenyl]-benzamide,

4-benzyl-N- [4- (2-diisopropylamino-ethoxy)-phenyl]-benzamide, 4-benzyl-N- [4-(2-diethylamino-ethoxy)-phenyl]-benzamide, 4'-ethyl-biphenyl-4-carboxylic acid [4- (2-diisopropylamino-ethoxy)-phenyl]-amide, and 4'-ethyl-biphenyl-4-carboxylic acid [4-(2-diethylamino-ethoxy)-phenyl]-amide.

A further aspect of this invention is those compounds of the Examples herein which are novel.

The compounds of formulae (I) to (IX), or their salts or solvates, are preferably in pharmaceutically acceptable or substantially pure form. By pharmaceutically acceptable form is meant, inter alia, of a pharmaceutically acceptable level of purity excluding normal pharmaceutical additives such as diluents and carriers, and including no material considered toxic at normal dosage levels.

Suitable salts and solvates include pharmaceutically acceptable salts and pharmaceutically acceptable solvates.

Suitable pharmaceutically acceptable salts include metal salts, such as for example aluminium, alkali metal salts such as lithium, sodium or potassium, alkaline earth metal salts such as calcium or magnesium and ammonium or substituted ammonium salts, for example those with lower alkylamines such as triethylamine, hydroxy alkylamines such as 2-hydroxyethylamine, bis- (2-hydroxyethyl)-amine or tri- (2-hydroxyethyl)-amine, cycloalkylamines such as bicyclohexylamine, or with procaine, dibenzylpiperidine, N-benzyl-p-phenethylamine, dehydroabietylamine, N, N'-bisdehydroabietylamine, glucamine, N-methylglucamine or bases of the pyridine type such as pyridine, collidine, quinine or quinoline.

Suitable pharmaceutically acceptable salts also includes pharmaceutically acceptable acid addition salts, such as those provided by pharmaceutically acceptable inorganic acids or organic acids.

Suitable pharmaceutically acceptable acid addition salts provided by pharmaceutically acceptable inorganic acids includes the sulphate, nitrate, phosphate, borate, hydrochloride and hydrobromide and hydroiodide.

Suitable pharmaceutically acceptable acid addition salts provided by pharmaceutically acceptable organic acids includes the acetate, tartrate, maleate, fumarate, malonate,

citrate, succinate, lactate, oxalate, benzoate, ascorbate, methanesulphonate, a-keto glutarate and a-glycerophosphate.

Suitable pharmaceutically acceptable solvates include hydrates.

A substantially pure form will generally contain at least 50% (excluding normal pharmaceutical additives), preferably 75%, more preferably 90% and still more preferably 95% of the compound of formula (I) to (IX) or its salt or solvate.

One preferred pharmaceutically acceptable form is the crystalline form, including such form in a pharmaceutical composition. In the case of salts and solvates the additional ionic and solvent moieties must also be non-toxic.

Examples of pharmaceutically acceptable salts of a compound of formula (I) to (IX) include the acid addition salts with the conventional pharmaceutical acids, for example, maleic, hydrochloric, hydrobromic, phosphoric, acetic, fumaric, salicylic, citric, lactic, mandelic, tartaric, succinic, benzoic, ascorbic and methanesulphonic.

The compounds of formula (I) to (IX) may exist in more than one stereoisomeric form, and the invention extends to all such forms as well as to their mixtures thereof, including racemates.

The compounds of formula (I) to (IX), or salts or solvates thereof, may be prepared by the methods illustrated in the following general reaction schemes, or by modification thereof, using readily available starting materials, reagents and conventional synthetic procedures.

If a particular enantiomer of a compound of the present invention is desired, it may be synthesised starting from the desired enantiomer of the starting material and performing reactions not involving racemization processes or it may be prepared by chiral synthesis, or by derivatisation with a chiral auxiliary, where the resulting diastereomeric mixture is separated and the auxiliary group cleaved to provide the pure desired enantiomers.

Alternatively, where the molecule contains a basic functional group, such as amino, or an acidic functional group, such as carboxy, diastereomeric salts are formed with an appropriate optically active acid or base, followed by resolution of diastereomeric salts by fractional crystallization and subsequent recovery of the pure enantiomers.

Compounds of formula (I) to (IX) may prepared by condensing suitably substituted aryl or heteroarylcarboxylic acids and suitably substituted anilines, which are commercially

available or synthesized by methods known to the art from commercially available starting materials, using methods known to the art. For example, suitably substituted aryl or heteroarylcarboxylic acids are treated with an activating reagent, such as thionyl chloride, at a suitable temperature, such as at reflux, to afford aryl or heteroarylcarbonyl chlorides, and the aryl-or heteroarylcarbonyl chlorides are condensed with suitably substituted anilines in the presence of a suitable base, such as diisopropylethylamine, in a suitable solvent, such as dichloromethane, to give compounds of formula (I).

In particular, the preparation of certain carboxamides of formula (I) in which R3 is H is disclosed in WO 99/01127 and WO 99/06146 mentioned above, and analogous methods of preparation may be used in the present invention. Many additional methods for converting a carboxylic acid to an amide are known, and can be found in standard reference books such as"Compendium of Organic Synthetic Methods", Vol. I-VI (published by Wiley-Interscience).

For example the compounds of formula (I) may be prepared by reacting a compound of formula (X) R5-Z-R4-COL (X) where L is a leaving group such as halogen, especially chlorine or bromine with a compound of formula (XI) where A, Z, R3, R4, R5 and Q are as defined for formula (I).

In this process, groups convertible to RI, R2, R3, R4 and R5 may be present during the coupling, and converted to RI, R2, R3, R4 and R5 after coupling. Also it may be convenient to convert one RI, R2, R3, R4 and R5 to another Rl, R2, R3, R4 and R5 group after coupling. In particular, ring formation between the groups Rl, X, Y, R2 or

the addition of suitable cyclic groups embodying Rl, X, Y, R2, may be performed after coupling.

Accordingly, there is provided a process for the preparation of a compound of formula (I), or a salt or solvate thereof, wherein R3 is methyl or ethyl which process comprises the reaction of a compound of formula (X) as hereinbefore defined with a compound of formula (XI) wherein A and Q are as hereinbefore defined and R3 is methyl or ethyl.

There therefore also provided a process for the preparation of a compound of formula (I), or a salt or solvate thereof, with the proviso that the following compounds are excluded ; N- [4- [2- [bis (l-methylethyl) amino] ethoxy]-2-fluorophenyl]- [1, 1'-biphenyl]-4- carboxamide, N-[4-[2-[bis (l-methylethyl) amino] ethoxy] phenyl]-[l, l'-biphenyl]-4-carboxamide, biphenyl-4-carboxylic acid [4- (2-diisopropylamino-ethoxy)-phenyl]-amide, N- [4- (2-diisopropylamino-ethoxy)-phenyl]-4-phenoxy-benzamide, N- [4- (2-diethylamino-ethoxy)-phenyl]-4-phenoxy-benzamide, N- [4- (2-diisopropylamino-ethoxy)-phenyl]-3-phenoxy-benzamide, N- [4- (2-diethylamino-ethoxy)-phenyl]-3-phenoxy-benzamide, 4-cyclohexyl-N- [4-(2-diisopropylamino-ethoxy)-phenyl]-benzamide, 4-cyclohexyl-N- [4- (2-diethylamino-ethoxy)-phenyl]-benzamide, 4-benzyl-N- [4- (2-diisopropylamino-ethoxy)-phenyl]-benzamide, 4-benzyl-N- [4- (2-diethylamino-etlzoxy)-phenyl]-benzamide, 4'-ethyl-biphenyl-4-carboxylic acid [4-(2-diisopropylamino-ethoxy)-phenyl]-amide, and 4'-ethyl-biphenyl-4-carboxylic acid [4- (2-diethylamino-ethoxy)-phenyl]-amide. which process comprises the reaction of a compound of formula (X) as hereinbefore defined with a compound of formula (XI) as hereinbefore defined.

The compounds of formula (XI) may be prepared in a number of ways, for example when X is O or S coupling an appropriately substituted nitrobenzene compound with a dialkyaminoalcohol or thiol, and converting the NO2 group to NH2 by hydrogenation in the presence of palladium catalyst (or with iron/ammonium chloride) before coupling with an acid chloride, for example as illustrated below: + NaH + i H N O 0-, N 0 H2/Pd on C 1 30 1 OH p + ou HO/N ''U 0 0 0 H Et3N cl HO Ng t CH2C'2 /_ O/ N ProcedureA51

Acid chlorides of formula (X) may be prepared from the corresponding acids which are commercially available or described in the literature or may be prepared by methods analogous to those of the literature.

Alternatively the acids of formula (X) may be prepared by combining moieties containing respectively R5 and R4 via Z.

This may also be achieved conveniently by first coupling a compound of R4-CO-L with the compound of formula (XI) followed by reaction with a compound R5-Z-L (or L-R4- CO-L with R5-Z). For example an amine of formula (XI) may be reacted with an appropriately substituted bromobenzoyl chloride which may be then reacted with, for example, an appropriately substituted phenyl moiety with a leaving group, or a cyclic amine, as in the following scheme : HAN OU N I O MDC \ CI * O Br N B(oH N ProcedureA93) z r > BINAP Pd (OAc) Z ETOH PhH CS2CO, DME Na CO Na2co3 Procedure A107 Pd (PPh3) a Q 0 N O I N O N 0 N 0 o) o) N N

BINAP = (S)- (+)-2, 2'-Bis (diphenylphosphino)-1, 1'-binaphthyl Similar reactions building up the structure of formula (I) may be carried out starting with the compound of formula (X) and adding the equivalent of formula (XI) in sections, as in the scheme below where an N-protecting group on Q, here a piperazine ring, may be removed after coupling the components of formula (I) and replacement by a desired substituent: T, i N p HZN O O ''-'-N jOy Et'cl zozo DIEA resin 9 tCH fH. . O TFA 9 O CH2CI2 N O TFA -E- N O i 'L EtOH Formaldehyde o AcOH Amberlyst BH3CN +° °z Procedure Hw Fizz NN:

In an alternative strategy for building up the compounds of formula (XI) before coupling, so as to introduce a hydroxy group in Y, an appropriately substituted nitrophenol is linked to an epoxy compound which is then reacted with an amine forming a group Q which is- O-Y (OH)-NR1R2, before coupling with R5-Z-R4-CO-L, as illustrated by: Et2NH o- °/NaH/DMF O, N CH2CI2 N Oz NEt O'I Ti (OPr) 4 /O z Ti (OiPr) 4 OH i Nos Nos EtOH i) Et3SiOTf I Et3N HCI/Et2O 0 inHn rt H,/PdonC N 0 NEt2 ci NEtz I CI HZN W O W/ \ N Ea l + i OH OU ProcedureG1 iii) MeOH/Na2CO3

Nos = p-nitrobenzenesulphonyl Novel compounds of formula (I) where the amide nitrogen is alkylated (R3 is methyl or ethyl) may be prepared by alkylating an anilide of formula (XI) before coupling with an acid chloride of formula (X), for example, by utilising the following reductive amination procedure: H CFis H N,-_aO i) EtO-OEt HNao Omet I TFA OU N ii) NaBH4/EtOH + O Et3N/0 0 EtgN/0 1-11 CH2cl2 ci xi i I o NiPr2 ProcedureE1

The compounds of formula (I) may be converted into their pharmaceutically acceptable salts by reaction with the appropriate organic or mineral acids.

Solvates of the compounds of formula (I) may be formed by crystallization or recrystallization from the appropriate solvent. For example, hydrates may be formed by crystallization or recrystallization from aqueous solutions, or solutions in organic solvents containing water.

Also salts or solvates of the compounds of formula (I) which are not pharmaceutically acceptable may be useful as intermediates in the production of pharmaceutically acceptable salts or solvates. Accordingly such salts or solvates also form part of this invention.

The above-listed compounds and pharmaceutically acceptable salts thereof, especially the hydrochloride, and pharmaceutically acceptable solvates, especially hydrates, form a preferred aspect of the present invention.

By virtue of the activity of these compounds as antagonists of a human 11 CBy receptor, the compounds of formula (I) are believed to have a role in preventing, ameliorating or correcting dysfunctions of diseases, including, but not limited to,"the Disorders" previously mentioned.

It is also considered that the treatment of certain of the Disorders mentioned above by an antagonist to the human 1 lCBy receptor are novel. Accordingly, the invention also provides a method for the treatment of diabetes, major depression, manic depression, anxiety, schizophrenia and sleep disorders, in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 ICBy receptor. In particular the the invention provides a method for the treatment of diabetes in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 lCBy receptor. In particular the invention provides a method for the treatment of major depression, in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 lCBy receptor. In particular the invention provides a method for the treatment of manic depression, in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 lCBy receptor. In particular the the invention provides a method for the treatment of anxiety in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 lCBy receptor. In particular the the invention provides a method for the treatment of schizophrenia in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 lCBy receptor.

In particular the the invention provides a method for the treatment of sleep disorders, in human or non-human mammals which method comprises the administration of a therapeutically effective amount of an antagonist to the human 1 lCBy receptor.

The administration of such compounds to a mammal may be by way of oral (including sub-lingual), parenteral, nasal, rectal or transdermal administration.

An amount effective to treat the Disorders hereinbefore described depends on the usual factors such as the nature and severity of the disorders being treated and the weight of the mammal. However, a unit dose will normally contain 1 to 1000 mg, suitably 1 to 500 mg, for example an amount in the range of from 2 to 400 mg such as 2,5,10,20,30,40, 50,100,200,300 and 400 mg of the active compound. Unit doses will normally be administered once or more than once per day, for example 1, 2,3,4,5 or 6 times a day, more usually 1 to 4 times a day, such that the total daily dose is normally in the range, for a 70 kg adult of 1 to 1000 mg, for example 1 to 500 mg, that is in the range of approximately 0.01 to 15 mg/kg/day, more usually 0.1 to 6 mg/kg/day, for example 1 to 6 mg/lcg/day.

It is greatly preferred that compounds of formula (I) are administered in the form of a unit-dose composition, such as a unit dose oral (including sub-lingual), nasal, rectal, topical or parenteral (especially intravenous) composition.

Such compositions are prepared by admixture and are suitably adapted for oral or parenteral administration, and as such may be in the form of tablets, capsules, oral liquid preparations, powders, granules, lozenges, reconstitutable powders, injectable and infusable solutions or suspensions or suppositories. Orally administrable compositions are preferred, in particular shaped oral compositions, since they are more convenient for general use.

Tablets and capsules for oral administration are usually presented in a unit dose, and contain conventional excipients such as binding agents, fillers, diluents, tabletting agents, lubricants, disintegrants, colourants, flavourings, and wetting agents. The tablets may be coated according to well known methods in the art.

Suitable fillers for use include cellulose, mannitol, lactose and other similar agents.

Suitable disintegrants include starch, polyvinylpyrrolidone and starch derivatives such as sodium starch glycollate. Suitable lubricants include, for example, magnesium stearate.

Suitable pharmaceutically acceptable wetting agents include sodium lauryl sulphate.

These solid oral compositions may be prepared by conventional methods of blending, filling, tabletting or the like. Repeated blending operations may be used to distribute the active agent throughout those compositions employing large quantities of fillers. Such operations are, of course, conventional in the art.

Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, gelatin, hydroxyethylcellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example, almond oil, fractionated coconut oil, oily esters such as esters of glycerine, propylene glycol, or ethyl alcohol ; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid; and if desired conventional flavouring or colouring agents.

Oral formulations also include conventional sustained release formulations, such as tablets or granules having an enteric coating.

For parenteral administration, fluid unit dose forms are prepared containing the compound and a sterile vehicle. The compound, depending on the vehicle and the concentration, can be either suspended or dissolved. Parenteral solutions are normally prepared by dissolving the compound in a vehicle and filter sterilising before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents are also dissolved in the vehicle. To

enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum.

Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and is sterilised by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound of the invention.

As is common practice, the compositions will usually be accompanied by written or printed directions for use in the medical treatment concerned.

Compounds of the present invention may be employed alone or in conjunction with other compounds, such as therapeutic compounds.

No adverse toxicological effects are expected for the compounds of the invention, when administered in accordance with the invention.

Accordingly, in a further aspect, the present invention provides a pharmaceutical composition for use in the treatment and/or prophylaxis of one or more of the Disorders which comprises a compound of this invention, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier.

The present invention also provides a method of treatment and/or prophylaxis of one or more of the Disorders comprising administering to the sufferer in need thereof an effective or prophylactic amount of a compound of this invention, or a pharmaceutically acceptable salt or solvate thereof.

In a further aspect the invention provides the use of a compound of this invention, or a pharmaceutically acceptable salt or solvate thereof, for the manufacture of a medicament for the treatment and/or prophylaxis of one or more of the Disorders.

In a still further aspect the invention provides the use of a novel compound of this invention, or a pharmaceutically acceptable salt or solvate, thereof as a therapeutic agent, in particular for the treatment and/or prophylaxis of one or more of the Disorders.

Compounds for use in this invention and their preparation are illustrated in the following Examples and Tables.

These Examples illustrate general procedures and sources of chemicals utilised to prepare compounds whose structures are shown in the Tables of data which follow the Examples.

In the case of Examples prepared as members of a coupled array, the synthetic origin of all starting componants of the array are shown in the Examples. Rather than detailing the experimental procedure for each case, the method by which individual members of the array were prepared is indicated in a Table by reference to a related Example. Mass spectral characterisation of all Examples is provided in the tables of data. Additional characterisation is provided for selected representative Examples with full experimental procedures.

Example Al [WO-00/06146] Utilising the procedure of Example A7 with 4-biphenylcarboxylic acid [Aldrich] in place of 2'-methyl-4-biphenylcarboxylic acid.

Example A2 Correspondingly Example A7 with 4- (5-methyl- [1, 2,4] oxadiazol-3-yl)-benzoic acid [J. Org. Chem. 50 ; 8; 1985; 1182].

Example A3 Correspondingly Example A7 with 4-pyrazol-1-yl-benzoic acid [Can. J. Chem.; 41 ; 1963; 1540].

Example A4 Correspondingly Example A7 with 3-biphenylcarboxylic [Med. Chem. Res. ; 6; 2 ; 1996].

Example A5

Correspondingly Example A7 with 4- (2-pyridyl)-benzoic acid [J. Chem. Soc.; 1940 ; 355, 356].

Example A6 Correspondingly Example A7 with 3'-acetyl-biphenyl-4-carboxylic acid [Patent WO- 9743262].

Example A7 2-methylphenyl-4-phenylcarboxylic acid [3-methoxy-4- (2-bis- (2-methylethyl) amino)- ethoxy)-phenyl amide.

To a solution of the acid (2'-methyl-biphenyl-4-carboxylic acid) [Patent WO-9901127] (55mg, 0.26mmol) in dimethylformamide were added (1- (3-dimethylaminopropyl)-3- ethyl carbodiimide hydrochloride [Aldrich] (50mg, 0.26mmol) and 1-hydroxy-7- azabenzotriazole [Aldrich] (35mg, 0.26mmol) followed by diisopropylethylamine (0.04ml, 0.25mmol) and the aniline (4- (2-diisopropylamino-ethoxy)-3-methoxy- phenylamine) (69mg, 0.22mmol), [prepared using the method used to form 3-methoxy-4- (2-pyrrolidin-1-yl-ethoxy)-phenylamine in Example A51 but with 2-diisopropylamino- ethanol in place of 1- (2-hydroxyethyl)-pyrrolidine]. The reaction mixture was stirred at room temperature for 16 hours. The solvent was evaporated, and the residue re-dissolved in dichloromethane (lOml), filtered through an SAX [Varian] column (2g), and the filtrate was then stirred with PS-isocyanate resin [Argonaut Technologies] (lOOmg, 0.38mmol) for 16 hours. The mixture was filtered, evaporated, and the residue purified by flash chromatography on silica gel using dichloromethane-aq. ammonia-methanol as eluent, to afford the title compound as an oil.

1H NMR (CDC13) : 8 1.04 (12H, d), 2.28 (3H, s), 2.90 (2H, t), 3.05 (2H, m), 3.91 (3H, s), 3.95 (2H, t), 6.88 (1H, d), 7.03 (1H, dd), 7.27-7. 32 (4H, m), 7.44, (2H, d), 7.53 (1H, d), 7.94 (2H, d) and 8.01 (1H, bs); MS (AP+ve): m/z 461 [M+H] +.

Example A8 Utilising the procedure of Example A7 with cyclohexyl-4-benzoic acid [Aldrich], in place of (2'-methyl-biphenyl-4-carboxylic acid).

Example A9

Correspondingly Example A7 with 4- (2-thienyl)-benzoic acid [J. Chem. Soc. Perkin Trans. l ; 17; 1992; 2203].

Example A10 Correspondingly Example A7 with 4- (1-methyl-1H-pyrazol-4-yl)-benzoic acid [Patent: WO-99064091.

Example All Correspondingly Example A7 with 4'- (5-methyl- [1, 2,4] oxadiazol-3-yl)-biphenyl-4- carboxylic acid [Patent : WO-9743262].

Example A12 Correspondingly Example A7 with 4-benzyl-carboxylic acid [Apin].

Example A13 Correspondingly Example A7 with 3'-cyano-biphenyl-3-carboxylic acid [J. Chem. Soc. PerlcinTrans. 2; 1; 1984; 35-38].

Example A14 Correspondingly Example A7 with 3'-methanesulfonyl-biphenyl-4-carboxylic acid [Izv. Sib. Otd. Akad. Nauk SSSR Ser. Khim. Nauk; 11; 1966; 62].

Example A15 Correspondingly Example A7 with 3-thiophen-2-yl-benzoic acid [Tetrahedron Lett.; 39; 24; 1998; 4175].

Example A16 Correspondingly Example A7 with 3-thiophen-3-yl-benzoic acid [J. Chem. Soc. B; 1970; 1595].

Example A17 Correspondingly Example A7 with 4-acetyl-4-biphenylcarboxylic acid [Aldrich].

Example A18 Correspondingly Example A7 with 4'-cyano-3'-methylbiphenyl-4-carboxylic acid [WO- 9850358].

Example A19 Correspondingly Example A7 with 4'- (5-methyl- [1, 3,4] oxadiazol-2-yl)-biphenyl-4- carboxylic acid [Patent : WO-9743262].

Example A20 Correspondingly Example A7 with 4-thiophen-3-yl-benzoic acid [J. Chem. Soc. B; 1970; 1595].

Example A21 Correspondingly Example A7 with 4-pyrazin-2-yl-benzoic acid [Patent WO-9854164].

Example A22 Utilising the procedures of Example A93 with 2-methoxyphenylboronic acid [Aldrich] in place of 4-methylphenylboronic acid, and Example A51 with 2- (diisopropylamino)- ethanol in place of 1- (2-hydroxyethyl)-pyrrolidine.

Example A23 Utilising the procedure of Example A22 with 4-trifluoromethylphenylboronic acid [Aldrich], in place of 2-methoxyphenylboronic acid [Aldrich] Example A24 Correspondingly Example A23 with 3-aminophenylboronic acid [Aldrich].

Example A25 Correspondingly Example A23 with 4-benzyloxyphenylboronic acid [Lancaster].

Example A26 Correspondingly Example A23 with 2-naphthylboronic acid [Lancaster].

Example A27 Correspondingly Example A23 with 3-naphthylboronic acid [Lancaster].

Example A28 Correspondingly Example A23 with 4-methylphenylboronic acid [Lancaster].

Example A29 Correspondingly Example A23 with 4-methylthiophenylboronic acid [Lancaster].

Example A30 Correspondingly Example A23 with 3-trifluoromethylphenylboronic acid [Lancaster].

Example A31 Correspondingly Example A23 with 4-carbonylphenylboronic acid [Aldrich].

Example A32 Correspondingly Example A23 with 3,4- (methylenedioxy) phenylboronic acid [Aldrich].

Example A33 Correspondingly Example A23 with 4-vinylphenylboronic acid [Aldrich].

Example A34 Correspondingly Example A23 with 3-methoxyphenylboronic acid [Lancaster].

Example A35 Utilising the procedure of Example A51 with 1- (2-hydroxyethyl) morpholine [Aldrich] in place of 1- (2-hydroxyethyl) pyrrolidine.

Example A36 Utilising the procedure of Example A35 with 4-cyclohexylbenzoic acid [Aldrich]. in place of 4-biphenylcarboxylic acid.

Example A37 Utilising the procedure of Example A51with2-dimethylaminoetllanol [Aldricll], in place of 1- (2-hydroxyethyl) pyrrolidine.

Example A39 Correspondingly Example A51 with (R)- (+)-l-methyl-2-pyrrolidinemethanol (Patent WO-9932480).

Example A41 Correspondingly Example A51 with 3-hydroxy-l-methylpiperidine [Aldrich].

Example A43 Correspondingly Example A51 with 2-dimethylamino-1-propanol [ICN-RF].

Example A45 Correspondingly Example A51 with 2- (diethylamino)-ethanol [Aldrich].

Example A47 Correspondingly Example A51 with (S)-(-)-1-methyl-2-pyrrolidinementhanol [Aldrich].

Example A49 Correspondingly Example A51 with N-benzyl-N-methylethanolamine [Aldrich].

Example A51 Biphenyl-4-carboxylic acid [3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl amide.

To a solution of the hydroxy amine, (1-(2-hydroxyethyl)-pyrrolidine) [Aldrich], (1.87ml, 16mmol) in dimethylformamide was added portionwise sodium hydride [60% dispersion in oil, (544mg, 16mmol). After stirring at room temperature for 10 minutes a solution of the halonitrobenzene, (1-chloro-2-methoxy-4-nitro-benzene) [Avocado] (3g, 16mmol) in dimethylformamide (lOml) was added dropwise. The reaction mixture was left stirring at room temperature for 16hrs then concentrated. The residue was dissolved in ethyl acetate

(200ml) and washed with water (3 x 50ml). The organic phase was dried with magnesium sulphate, evaporated and the residue purified by flash chromatography on silica gel using dichloromethane-aq. ammonia-methanol as eluent to afford 1- [2- (2-methoxy-4-nitro- phenoxy)-ethyl]-pyrrolidine as a brown oil.

1H NMR (CDC13) : 81. 82 (4H, m), 2.65 (4H, m), 3.01 (2H, t), 3.94 (3H, s), 4.24 (2H, t), 6.92 (1H, d), 7.74 (1H, d), and 7.89 (1H, dd); MS (AP +ve): m/z 267 [M+H] +.

To a solution of the amine, 1-[2-(2-methoxy-4-nitro-phenoxy)-ethyl]-pyrrolidine (2.3g, 8.6mmol) in ethanol (100ml) was added 10% Pd/C (50mg). The mixture was stirred at room temperature under an atmosphere of hydrogen at atmospheric pressure for 16h, then filtered through celite and the filtrate concentrated to give the corresponding aniline ; 3- methoxy-4- (2-pyrrolidin-1-yl-ethoxy)-phenylamine, as a brown solid.

1H NMR (CDC13): 8 1.80 (4H, m), 2.62 (4H, m), 2.89 (2H, t), 3.80 (3H, s), 4.06 (2h, t), 6.20 (1H, dd), 6.29 (1H, d) and 6.75 (1H, d); MS (AP +ve) : nz/z 237 [M+H] +.

To the carboxylic acid, (4-biphenyl carboxylic acid) [Aldrich] (47.5mg, 0.24mmol) suspended in dichloromethane (lml) was added oxalyl chloride [Aldrich] (0.06ml, 0.72mmol) followed by one drop of dimethylformamide. The reaction mixture was stirred at room temperature for 1 hour, concentrated, then co-evaporated three times with dichloromethane to give 4-phenylbenzoyl chloride. This was dissolved in dichloromethane (lml) and added to a solution containing the amine, (3-methoxy-4- (2- pyrrolidin-l-yl-ethoxy)-phenylamine), (47 mg, 0. 2mmol), triethylamine (0.14ml, lmmol) and dichloromethane (lml). The reaction mixture was stirred for 16 hours at room temperature, concentrated, re-dissolved in dichloromethane (lOml), filtered through an SAX column [Varian] (2g) and stirred with PS-isocyanate resin [Argonaut Technologies] (100mg, 0.38mmol) for 16 hours. The mixture was filtered, evaporated then purified by flash chromatography on silica gel using dichloromethane-aq. ammonia-methanol as eluent to afford the title compound as an oil.

IH NMR (CDCl3) : 6 1. 88 (4H, m), 2.90 (4H, m), 3.08 (2H, t), 3.84 (3H, s), 4.21 (2H, t), 6.83 (1H, d), 7.03 (1H, dd), 7.27-7.70 (8H, m) and 8.01 (2H, d); MS (AP+ve): m/z 417 [M+H].

Example A54

Utilising the procedure of Example A51 with 1-dimethylamino-2-propanol [Aldrich] in place of 1-(2-hydroxyethyl)-pyrrolidine.

Example A56 Correspondingly Example A51 with 1- (2-hydroxyethyl)-piperidine [Aldrich].

Example A58 Correspondingly Example A51 with 2- (hexamethyleneamino)-ethanol [Lancaster].

Example A60 Utilising the procedures of Example A93 with 3-aminophenylboronic acid in place of 2- methoxyphenylboronic acid and Example 51 with 2-dimethylaminoethanol in place of 1- (2-hydroxyethyl) pyrrolidine.

Example A63 Utilising the procedure of Example A60 with 4-carboxyphenylboronic acid [Aldrich] in place of 3-aminophenylboronic acid.

Example A70 Correspondingly Example A63 with (3,4-methylenedioxyphenyl) boronic acid [Aldrich].

Example A72 Utilising the procedure of Example 51 with N (2-phenyl)-ethyl-N-methyl-ethanolamine [J. Org. Chem. 1985,50 (22), 4359] in place of 1- (2-hydroxyethyl)-pyrrolidine.

Example A74 Correspondingly Example 51 with 2-dimethylaminocyclohexanol [J. Chem. Soc. C (1969), (2), 248-52].

Example A76 Correspondingly Example 51 with 2- (1, 2,4,5-tetrahydro-benzo [d] azepin-3-yl)-ethanol [Patent US-394682]

Example A78 Correspondingly Example 51 with 2- (3, 4-dihydro-lH-isoquinolin-2-yl)-ethanol [Patent WO-9719926].

Example A80 Correspondingly Example 51 with 2- (4-phenyl-piperazin-1-yl)-ethanol [J. Med. Chem. 1994,37 (13), 1964].

Example A82 Correspondingly Example 51 with 1-methyl-3-pyrrolidinol [Aldrich].

Example A84 Utilising the procedures of Example A93 with 4-methoxy-phenylboronic acid [Aldrich] in place of 2-methoxyphenylboronic acid and Example A51 with 2-diethylaminoethanol in place of 1- (2-hydroxyethyl) pyrrolidine.

Example A88 Utilising the procedures of Example A84 with 4-methoxy-3-pyridylboronic acid [Patent WO-9924440] in place of 4-methoxy-phenylboronic acid.

Example A89 Correspondingly Example A88 with 2-methoxy-3-pyridylboronic acid [Patent WO- 9910331].

Example A90 Correspondingly Example A88 with benzo- [b]-furan-2-boronic acid [Aldrich].

Example A91 Correspondingly Example A88 with thiophene-3-boronic acid [Aldrich].

Example A92 Correspondingly Example A88 with indole-5-boronic acid [Frontier].

Example A93 4'-Methyl-biphenyl-4-carboxylic acid [3-methoxy-4- (2-pyrrolidin-1-yl-ethoxy)- phenyl]-amide A mixture of 3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenylamine [Example A51] (4.7mM l. lg) and triethylamine (14mmol) was treated with 4-bromobenzoyl chloride [Aldrich] in dichloromethane (20ml) and kept at room temperature for 16 hours.

The solvent was evaporated and the crude product purified by chromatography on silica gel using dichloromethane-methanol-aq. ammonia to afford 4-bromo -N-L3-methoxy-4 (2-pyrrolidin-1-yl-ethoxy)-phenyl]-benzamide as a white solid in 72% yield.

1H NMR (DMSO-d6): 8 7.91 (2H, dd), 7.73 (2H, dd), 7.50 (1H, d), 7. 30 (1H, dd), 6.94 (1H, d), 4.02 (2H, t), 3.76 (3H, s), 2.77 (2H, t), 2.51 (4H, m under DMSO-d-5 signal) and 1.67 (4H, m) ; MS: (ES+ve) tWz 419,421 [M+H] + The amide, 4-bromo-N [3-methoxy-4 (2-pyrrolidin-1-yl-etlioxy)-phenyl]-benzamide (O. lmM 42mg), and 4-methyl-benzene boronic acid [Aldrich] (O. 1mM 14mg) were refluxed for 16 hours in a mixture of benzene (8ml), ethanol (2ml) and 2M aqueous sodium carbonate (2ml) in the presence of tetrakis-(triphenylphosphine)-palladium [O] (5mg) under an argon atmosphere. The mixture was cooled, the upper layer decanted, and this solution purified by chromatography on silica gel using dichloromethane: methanol (10: 1) followed by acetonitrile: satd. aqueous ammonia (25: 1) to afford the title compound as a white solid.

1H NMR (CDC13) : 8 7.92 (2H, dd), 7.68 (2H, dd), 7.50 (2H, dd), 7.26 (3H, dddd), 6.96 (1H, dd), 6.88 8 (1H, d), 4.13 (1H, t), 3.87 (3H, s), 2.92 (2H, t), 2.60 (4H, m), 2.41 (3H, s) and 1.80 (4H, m); MS: (AP-ve) m/z 429 [M-H]- ; (AP+ve) m/z 431 [M+H] +.

Example A100 Utilising the procedure of Example A93 with 4- (2, 6-dimethoxypyrimidinyl)-boronic acid [Frontier] in place of 4-methyl-benzene boronic acid.

Example A103 Correspondingly Example A93 with furan-3-boronic acid [Frontier].

Example A104 Correspondingly Example A93 with mesityl-boronic acid [Frontier].

Example A105 Utilising the procedure of Example A51 except employing chloroform in place of dichloromethane as a solvent and eluent and utilising 3-quinuclidinol [Aldrich] in place of 1-(2-hydroxyethylpyrrolidine) Example A107 Utilising the procedure of Example B37 except using piperidine in place of aniline.

Example B1 Utilising the procedure of Example A7 with 3-phenoxybenzoic acid [Aldrich] in place of 2'-methyl-biphenyl-4-carboxylic acid.

Example B2 Correspondingly Example B1 using 4-benzylbenzoic acid [Apin].

Example B34 Correspondingly Example Bl using 3-benzylbenzoic acid [Patent WO-9828268].

Example B35 Correspondingly Example B1 using 4-phenoxybenzoic acid [Aldrich].

Example B37 N [- [3-Methoxy-4- (2-pyrrolidin-1-yl-ethoxy)-phenyl]-4-phenylamino-benzamide Dry cesium carbonate (0. 15mM, 49mg), (S)-BINAP [Aldrich] (0.015 mM, 9mg) and palladium acetate (0.0075mM, 2mg) were sonicated in anhydrous ethyleneglycol

dimethyl ether (15 ml) for 40 minutes under an argon atmosphere. This suspension was treated with 4-bromo-N-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-be nzamide [Example A93] (O. lrnM, 42mg) and aniline (0. 1 lmM, lOmg) then refluxed for 40 hours.

The suspension was filtered through a hydrophobic membrane, concentrated, then purified on C 18 R. P. silica using acetonitrile: water to afford the title compound as a white solid.

1HNMR (MeOH-d4) : 6 7.96 (2H, dd) 7.92 (1H, d), 3.1 (2H, dd), 7.20 (1H, dd), 7.04 (1H, d), 4.28 (2H, t), 3.92 (3H, s), 3.78 (2H, m), 3.60 (2H, t), 3.58-3.13 (6H, m) and 2.26- 1.47 (1OH, m) ; MS: (ES+ve) xz/z 424 [M+H] + Example Cl Utilising the procedure of Example A7 with 2-methylbiphenyl-4-carboxylic acid [Patent WO-9606079] in place of 2'-methyl-biphenyl-4-carboxylic acid.

Example C2 Correspondingly Example Cl using 3-methoxybiphenyl-4-carboxylic acid [Patent WO- 9534540].

Example C3 Correspondingly Example Cl using 3-methylbiphenyl-4-carboxylic acid [Patent WO- 9534540].

Example C4 Correspondingly Example Cl using 4-phenylthiophene-2-carboxylic acid [Specs].

Example C5 Correspondingly Example Cl using 4- (3, 5-dichlorophenoxy)-furan-2-carboxylic acid [Maybridge].

Example C6 Correspondingly Example Cl using 5-methyl-1-phenylpyrazole-4-carboxylic acid [Maybridge].

Example C7 Correspondingly Example Cl using 6-phenyl-nicotinic acid [WO-0006085].

Example C8 Correspondingly Example Cl using 3-chloro-biphenyl-4-carboxylic acid [Patent JP- 09221476].

Example C9 Correspondingly Example Cl using 5- (4-chlorophenyl)-2-trifluoromethyl-furan-3- carboxylic acid [Maybridge].

Example C10 Correspondingly Example Cl using 2- (4-chlorophenyl)-3- (trifluoromethyl)-pyrazole-4- carboxylic acid [Maybridge].

Example Cll Correspondingly Example Cl using 5- (2-pyridyl)-thiophene-2-carboxylic acid [Maybridge].

Example C12 Correspondingly Example Cl using 5- (methyl-trifluoromethyl-2-H-pyrazol-3-yl)- thiophene-2-carboxylic acid [Maybridge].

Example Dl Utilising the procedure of Example D5 with 3,4-dichloronitrobenzene [Aldrich] in place of 2, 4-dichloronitrobenzene.

Example D5 <BR> <BR> <BR> Biphenyl-4-carboxylic acid [2-chloro-4- (2-diisopropylamino-ethoxy)-phenyl]-amide.

To a three-neck flask (fitted with condenser, dropping funnel and thermometer) containing iron powder (938mg, 16.8mmol) mixed with a solution of ammonium chloride

(28mmol) in water (28ml), was added the amine [2- (3-chloro-4-nitro-phenoxy)-ethyl]- diisopropyl-amine [prepared by the method used to form 1- [2- (2-methoxy-4-nitro- phenoxy)-ethyl]-pyrrolidine in Example A51 but with 2-4-dichloronitrobenzene [Aldrich] in place of 4-cliloro-3-methoxynitrobenzene and 2-diisopropylaminoethanol in place of 1-(2-hydroxylethyl)-pylTolidine], dropwise over 10 minutes. The reaction mixture was gently refluxed until t. l. c. analysis showed no starting material. The mixture was filtered while hot and the inorganic residues washed with methanol. The combined filtrates were partitioned between water (5ml) and ethyl acetate (3 x 10ml), the organic phase dried (MgS04), filtered, and evaporated. The aqueous phase was treated with satd. aq. sodium bicarbonate (lOml), extracted with ethyl acetate (3 x 10ml), dried (MgS04), and evaporated. Residues from both extractions were combined and purified by flash chromatography on silica gel using dichloromethane-methanol-aq. ammonia as eluent to afford 2-chloro-4- (2-diisopropylamino-ethoxy)-phenylamine as a brown oil.

1H NMR (CDC13) : 8 1.02 (12H, d), 2.77 (2H, t), 3.03 (2H, sept.), 3.72 (2H, bs), 3.80 (2H, t), 6.68 (2H, m) and 6.85 (1H, m); MS (AP+ve): m/z 271,273 [M+H] +.

This material was used in place of 3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenylamine in the procedure of Example A51 to afford the title compound as clear oil.

1H NMR (CDC13) : 8 1.26 (12H, d), 3.07 (2H, m), 3.35 (2H, m), 4.22 (2H, m), 6.89 (1H, dd), 7.01 (1H, m), 7.44 (3H, q), 7.62 (2H, d), 7.71 (2H, d), 7.97 (2H, d) and 8. 34 (1H, d); MS (AP+ve): m/z 452,454 [M+H] +.

Example D9 Utilising the procedure of Example A51 with 2,4-difluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene.

Example D12 [WO-00/06146] Utilising the procedure of Example A51 with 3,4-difluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene.

Example D16

Utilising the procedure of Example A51 with 2-methyl-4-fluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene Example D20 Utilising the procedure of Example A51 with 3-methyl-4-fluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene Example D24 Utilising the procedure of Example A51 with 3-acetyl-4-fluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene Example D25 Biphenyl-4-carboxylicacid [4- (2-diisopropylamino-ethoxy)-2-formyl-5-methoxy- phenyl]-amide Biphenyl-4-carboxylic acid [4- (2-diisopropylamino-ethoxy)-3-methoxy-phenyl]-amide [Patent WO-9901127] (223 mg, 0.5 mmol) was treated with glyoxylic acid trihydrate (lml), dichloromethane (5 ml) and methanesulphonic acid (0.5 ml). The mixture was stirred vigorously for 24 hours then treated with satd. aq. sodium bicarbonate (30ml) and extracted with dichloromethane (3 x 20ml). The combined organic phases were dried (MgSOq.), filtered and evaporated, then subjected to flash chromatography on silica gel [chloroform-methanol-aqueous acetic acid] to obtain the title compound as the acetate salt, a white solid.

1H NMR (CDC13) : 5 1.13 (12H, d), 2.04 (3H, s), 3.02 (2H, t), 3.20 (2H, hept.), 4.05 (3H, s), 4.10 (2H, t), 5.0 (1H, bs), 7.22 (1H, s), 7.40 (1H, t), 7.48 (2H, d), 7.65 (2H, d), 7.76 (2H, d), 8.14 (2H, d), 8.72 (1H, s) and 9.34 (1H, s); MS (AP+ve): m/z 475 [M+H+].

Example D26 <BR> <BR> <BR> <BR> Biphenyl-4-carboxylic acid [4- (2-diethylamino-ethoxy)-3- (1-hydroxy-ethyl)-phenyl]- amide To biphenyl-4-carboxylic acid [3-acetyl-4- (2-diethylamino-ethoxy)-phenyl]-amide [Example D24] (20mg, 0. 05mmol) dissolved in a 1: 1 mixture of tetrahydrofuran/ ethanol (3ml), was added sodium borohydride [Aldrich] (6mg, 0. 15mmol). The reaction mixture was stirred at ambient temperature for 16 hours. The solvent was evaporated and

the residue purified by flash chromatography on silica gel using dichloromethane-aq. ammonia-methanol as eluents, to afford the title compound as a white solid. lHNMR (CDC13) : 8 1.09 (6H, t), 1.49 (3H, d), 2.75 (4H, q), 2.95 (2H, t), 4.15 (2H, t), 5.01 (1H, q), 6.84 (1H, d), 7.45-7.67 (9H, m) and 7.95 (2H, d) MS (AP+ve): nzlz 433 [M+H+].

Example D27 Biphenyl-4-carboxylic acid [4- (2-diethylamino-ethoxy)-3-ethyl-phenyl]-amide To biphenyl-4-carboxylic acid [3-acetyl-4- (2-diethylamino-ethoxy)-phenyl]-amide [Example D24] (25mg, 0.06mmol) dissolved in dichloromethane (1. 5ml), was added triethylsilane (0. 5ml) and trifluoroacetic acid (0.25ml). The resulting yellow solution was stirred at room temperature for 120h. The solvents were evaporated and the residue purified by flash chromatography on silica gel using dichloromethane-aq. ammonia- methanol as eluents to afford the title compound as white solid.

1H NMR (CDC13) : 6 1. 17 (6H, m), 2.64 (2H, q), 2.8 (4H, q), 3.06 (2H, t), 4.15 (2H, t), 6.82 (1H, d), 7.35-7.71 (9H, m) and 7.96 (2H, d) MS (AP+ve): m/z 417 [M+H] + Example D28 [W09901127] Utilising the procedure of Example A51 with 4-fluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene, and 2-diisopropylaminoethanol in place of 1- (2- hydroxyethyl)-pyrrolidine Example D30 [W09901127] Utilising the procedure of Example D28 with 2-dimethylaminoethanol [Aldrich] in place of2-diisopropylarninoethanol.

Example D32 [W09901127] Utilising the procedure of Example D28 with 2-diethylaminoethanol [Aldrich] in place of 2-diisopropylaminoethanol Example D38 [W09901127]

Utilising the procedure of Example A22 with 4-fluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene, and 4-ethylphenylboronic acid in place of 4- methoxyphenylboronic acid Example D39 [WO9901127] Utilising the procedure of Example A84 with 4-fluoronitrobenzene [Aldrich] in place of 4-chloro-3-methoxynitrobenzene, and 4-ethylphenylboronic acid in place of 4- methoxyphenylboronic acid.

Example E1 Biphenyl-4-carboxylic acid [4-(2-diisopropylamino-ethoxy)-3-methoxy-phenyl]- methyl-amide.

To 4- (2-diisopropylamino-ethoxy)-3-methoxy-phenylamine (lmmol) [Example A7] were added triethylorthoformate (8ml) and trifluoroacetic acid (0. 15ml). The resulting solution was heated to 90°C for 4hr. The solution was evaporated then redissolved in ethanol and cooled to approximately-10°C. Sodium borohydride (190mg, 5mmol) was introduced portionwise over 10 minutes then the mixture allowed to warm to room temperature. The solution was stirred at room temperature for 16h, then acidified to pH 1 with 2M hydrochloric acid. The mixture was concentrated to approximately 10ml, then partitioned between ethyl acetate and water. The aqueous phase was adjusted to pH 14 using 2M aq sodium hydroxide solution, and extracted with dichloromethane (x3), dried (MgS04), filtered and evaporated. The residue was purified by flash chromatography on silica gel using dichloromethane-aq. ammonia-methanol as eluent to afford [4- (2- diisopropylamino-ethoxy)-3-methoxy-phenyl]-methyl-amine as an oil. lH NMR (CDC13) : 8 1.03 (12H, d), 2.80 (3H, s), 2.85 (2H, t), 3.02 (2H, q), 3.80 (3H, s), 3.86 (2H, t), 6.13 (1H, dd), 6.23 (1H, d) and 6.80 (1H, d); MS (AP+ve): n2/z 281 [M+H] +.

To 4-phenylbenzoic acid (0.2mmol) suspended in dichloromethane was added oxalyl chloride (0.6mmol) followed by dimethylformamide (1 drop). The reaction mixture was stirred for lh, evaporated, co-evaporated (x3) with dichloromethane then redissolved in dichloromethane (lml). A solution containing the amine [4- (2-diisopropylamino-ethoxy)- 3-metlooxy-phenyl]-methyl-amine (0.2mmol) and triethylamine (140mg, lmmol) dissolved in dichloromethane (Iml) was added. This solution was stirred at ambient

temperature for 14 hours, evaporated, dissolved in dichloromethane (Iml) and treated with PS-isocyanate resin [Argonaut Technologies] (150mg). After a further 18h shaking at ambient temperature, the mixture was filtered, passed through an SAX column [Varian] (lg), evaporated, and the residue purified by chromatography on silica gel using dichloromethane-aq. ammonia-methanol as eluent to afford the title compound as an oil.

'H NMR (CDC13): 8 1.21 (12H, bd), 2.88-3.24 (4H, m), 3.32 (3H, s), 3.87 (3H, s), 4.11 (2H, m), 6.82-6.91 (3H, m) and 7.26-7.56 (9H, m) ; MS (AP+ve): ntlz 476 [M+H] +.

Example E5 Utilising the procedure of Example EI with triethyl orthoacetate [Aldrich] in place of triethyl orthoformate.

Example E12 Biphenyl-4-carboxylic acid [2-chloro-4-(2-diisopropylamino-ethoxy)-5-methoxy- phenyl]-amide Biphenyl-4-carboxylic acid [4- (2-diethylamino-ethoxy)-3-methoxy-phenyl]-methyl- amide [Example E9] (45mg, 0.1 mmol), was dissolved in chloroform (lml) and treated with benzotriazole [Aldrich] (12 mg, 0. 1 mmol) and N-chlorosuccinimide (13 mg, 0. 1 lmmol). The mixture was stirred at ambient temperature for 16 hours then evaporated and subjected to flash chromatography on silica gel (dichloromethane-methanol- aqueous ammonia) to afford the title compound as an oil.

'H NMR (CDC13) : 8 1.06 (6H, t), 2.63 (4H, q), 2.90 (2H, t), 3.39 (3H, s), 3.67 (3H, s), 4.03 (2H, t), 6.57 (1H, s), 6.84 (1H, s) and 7.31-7.53 (9H, m) ; MS (AP+ve): milz 467, 469 [M+H] +.

Example E13 Utilising the procedures of Example A93 with [4-(2-diethylamino-ethoxy)-3-methoxy- phenyl]-methyl-amine [Example E9] in place of 4-(2-diethylamino-ethoxy)-3-methoxy- phenylamine and 2-fluoromethylphenylboronic acid [Aldrich] in place of 4- methoxyphenylboronic acid and of Example 51 with (N-diethyl) ethanolamine in place of 1- (2-hydroxyethyl) pyrrolidine.

Example E14 Utilising the procedure of Example E13 with 2-methylphenylboronic [Aldrich] in place of of 4-chlorophenylboronic acid.

Example E16 Correspondingly Example E14 with 2-chloromethylphenylboronic acid [Aldrich].

Example E17 Correspondingly Example E14 with 4-fluoromethylphenylboronic acid [Aldrich].

Example E21 Correspondingly Example E14 with 4-chloromethylphenylboronic acid [Aldrich].

Example E22 Correspondingly Example E14 with 4-ethylphenylboronic acid [Aldrich].

Example E23 Correspondingly Example E14 with 4-te7 » tbutylphenylboronic acid [Aldrich].

Example E24 4-Biphenylcarboxylic acid [4- (2-diethylamino-ethoxy)-3-methoxy-phenyl]-methyl-amide [Example E9] (45mg, O. lmmol), was dissolved in acetonitrile (lml) and treated with N- fluoro-N'-chloromethyl-triethylenediamine-bis (tetrafluoroborate) (43mg, 0.12mmol) and heated to 80°C for 6 hours. The solvent was evaporated and the residue subjected to flash chromatography on silica gel (dichloromethane-methanol-aqueous ammonia) to afford the title compound as an oil.

MS (AP+ve): nVz 451 [M+H] +.

Example E25 Utilising the procedure of Example El with 4- (2-diisopropylamino-ethoxy)-3-methyl- phenylamine [Example D20] in place of 4- (2-diisopropylamino-ethoxy)-3-methoxy- phenylamine and triethyl orthoacetate in place of triethyl orthoformate.

Example F1 Utilising the procedure of Example A7 with 6-phenyl-nicotinic acid (Patent WO- 0006085) in place of 2'-methyl-4-biphenylcarboxylic acid and N-dimethylethanolamine in place of 2- (diisopropylamino) ethanol.

Example G1 Biphenyl-4-carboxylic acid [4- ( (R)-diethylamino-hydroxy-propoxy)-3-methoxy- phenyl]-amide 4-Nitro-2-methoxyphenol [Aldrich] (845mg, 5mmol) was dissolved in DMF (25 ml) and treated with sodium hydride (60% oil dispersion, 200mg). When the effervescence ceased, the mixture was treated with (R)-p-nitrophenylsulphonyl glycidol [Aldrich] and warmed to 50°C with stirring. After 16 hours, the mixture was cooled, evaporated, partitioned between water (20ml) and dichloromethane (3 x 25ml), dried (MgS04), filtered and evaporated. The residue was purified by flash chromatography on silica gel (hexane-ether) to give (R)-2- (2-methoxy-4-nitro-phenoxymethyl)-oxirane as a pale brown solid in 80% yield.

1H NMR (CDC13) : 6 2.79 (1H, dd), 2.95 (1H, dd), 3.41 (1H, dddd), 3.96 (3H, s), 4.06 (1H, dd), 4.43 (1H, dd), 6.98 (1H, d), 7.75 (1H, d) and 7.87 (1H, dd).

(R)-2-(2-Methoxy-4-nito-phenoxymethyl)-oxirane (O. Smmol, 113mg), in dichloromethane (3 ml) was treated with the amine (diethylamine) [Aldrich] (1. 5 mmol, 110 mg) and titanium tetraisopropoxide [Aldrich] (50ul). The solution was stirred at ambient temperature for 24 h, treated with water (lml) and shaken vigorously for 10 minutes. The resulting suspension was passed through a hydromatrix cartridge [Varian ChemElut] (5ml) eluting with dichloromethane (10ml) to give (R)-diethylamino- (2- methoxy-4-nitro-phenoxy)-propan-2-ol as a yellow oil 1H NMR (CDC13) : 5 1.07 (6H, t), 2.55-2.72 (7H, m), 3.94 (3H, s), 4.09-4.13 (3H, m), 6.97 (1H, d), 7.74 (1H, d) and 7.89 (1H, dd); MS (AP+ve) : 7nez 299 [M+H+].

This material was dissolved in ethanol (5ml) and treated with hydrogen chloride (2M in diethyl ether) 0.1 ml then 10% palladium on charcoal (20 mg) and hydrogenated at atmospheric pressure for 24 hours. The solution was purged with argon then filtered

through celite and evaporated to give (R)- (4-amino-2-methoxy-phenoxy)-diethylamino- propan-2-ol hydrochloride as a white crystalline solid.

'H NMR (CD30D): 5 1.19 (6H, t), 3.36-3.45 (6H, m), 3.88 (s, 3H), 4.02-4.11 (2H, m), 4.03 (1H, m), 6.95-7.03 (2H, m) and 7.13 (1H, d).

A solution of this material in dichloromethane (2ml) was treated with triethylamine (2 mmol, 280ul) and triethylsilyl trifluoromethanesulphonate (lmmol, 264mg). After 30 minutes, 4-biphenylcarboxylic acid chloride Example 1] (Immol, 217mg) was introduced and the mixture stirred for 12 hours. The solvent was evaporated and the residue dissolved in methanol (100ml) and treated with potassium carbonate (2g). After stirring for six hours, the suspension was evaporated, formed into a slurry with dichloromethane (20ml), filtered, the filtrate evaporated, and the residue purified by flash chromatography (dichloromethane-methanol-aq. ammonia) to give the title compound as a white solid.

1H NMR (CDC13) : 8 1.11 (6H, t), 2.61-2.78 (6H, m,), 3.88 (3H, s), 3.5-4.5 (1H, vbs), 3.99-4.13 (3H, m), 6.92 (1H, d), 6.99 (1H, dd), 7.41-7.49 (3H, m), 7.56 (1H, d), 7.63 (2H, d), 7.69 (2H, d) and 7.97 (3H, d); MS (AP+ve) :/z 449 [M+H].

Example G5 Utilising the procedure for the preparation of (R)-diethylamino- (2-methoxy-4-nitro- phenoxy)-propan-2-ol [Example Gl] but replacing dichloromethane with 1,2- dichloroethane and diethylamine with diisopropylamine. In addition, the mixture of amine and epoxide was heated at 80°C for 12h rather than being kept at ambient temperature for 24 hours.

Example G8 Utilising the procedure of Example G1 but using (S)-p-nitrophenylsulphonyl-glycidol in place of (R)-p-nitrophenylsulphonyl-glycidol, and pyrrolidine in place of diethylamine.

Example G22 Utilising the procedure of Example A51 but using 4-dimethylamino-1-butanol [ICN-RF] in place of 1- (2-hydroxyethyl)-pyrrolidine.

Example Hl 4-Cyclohexyl-N-[3-methoxy-4- (4-methyl-piperazin-1-yl)-phenyl]-benzamide A solution of 1-(2-methoxy-4-nitro-phenyl)-piperazine (Patent WO-9906382) (10mmol, 2.37g) in dichloromethane (50ml) was treated with ditertbutyl dicarbonate (lOmmol, 2.18g) with stirring. Vigorous evolution of gas occurred which ceased after 1 hour. The solution was then evaporated to a yellow solid 4-(2-methoxy-4-nitro-phenyl)-piperazine- 1-carboxylic acid tertbutyl ester. lHNMR (CDCl3) : 5 1.50 (9H, s), 3.16 (4H, t), 3.61 (4H, t), 3.96 (3H, s), 6.88 (1H, d), 7.72 (1H, d) and 7.86 (1H, dd).

This material was dissolved in ethanol (50ml) and treated with 10% Pd on carbon (l00mg). The suspension was hydrogenated at 1 atmosphere for 2 hours, then filtered through celite and evaporated to give 4- (4-amino-2-methoxy-phenyl)-piperazine-I- carboxylic acid tertbutyl ester as a brown oil.

1H NMR (CDC13) : 8 1.48 (9H, s), 2.86-2.91 (4H, t), 3.52-3.60 (4H, t), 3.81 (3H, s), 6.22- 6.27 (2H, m) and 6.73 (1H, d).

This aniline (0.2mmol, 61 mg) was dissolved in dichloromethane (lml) and treated successively with DIEA resin [Argonaut Technologies] (0.5 g) and 4-cyclohexylbenzoyl chloride [Example A36]. The mixture was shaken gently for 12 hours then filtered, evaporated and the residue purified by flash chromatography on silica gel (dichloromethane-methanol-aq. ammonia) to afford 4- (4- { [1- (4-cyclohexyl-phenyl)- methanoyl]-amino}-2-methoxy-phenyl)-piperazine-1-carboxylic acid tertbutyl ester as a white crystalline solid tH NMR (CDC13) : 8 1.25-1.47 (5H, m), 1.54 (9H, s), 1.75-1.88 (5H, m), 2.56 (1H, m), 2.98 (4H, t), 3.61 (4H, t), 3.91 (3H, s), 6.87 (1H, d), 6.93 (1H, dd), 7.32 (2H, d), 7.54 (1H, s), 7.77, (1H, s) and 7.78 (2H, d); MS (AP+ve): m/z 493 [M+H+].

This material was dissolved in dichloromethane (5ml) and treated with anisole (lml) and trifluoroacetic acid (5ml). After 2 hours the solution was evaporated, then co-evaporated twice from toluene. The residue was dissolved in dichloromethane (lOml), washed with satd. sodium bicarbonate (2ml), the organic phase dried (MgS04), filtered and evaporated to a brown oil, 4-cyclohexyl-N-(3-methoxy-4-piperazin-1-yl-phenyl)-benzamide .

1H NMR (CDCl3) : 8 1.22-1.87 (10, m), 2.57 (1, m), 3.04-3. 12 (8H, m), 3.91 (3H, s), 6.95 (2H, bs), 7.32 (2H, d), 7.54 (1H, m), 7.77 (1H, s) and 7.78 (2H, d); MS (AP+ve): nilz 394 [M+H+].

This amine (0. 1 mmol, 39mg) was dissolved in ethanol (3ml) and treated with metaformaldehyde (I OOmg), Amberlyst cyanoborohydride resin [Novabiochem] (lOOmg), and acetic acid (50ul). The mixture was stirred at ambient temperature for three hours then filtered, evaporated and the residue purified by flash chromatography on silica gel (dichloromethane-methanol-aq. ammonia) to afford the title compound as a pale brown oil. This was evaporated from dilute acetic acid to give the monoacetate salt hydrate.

1H NMR (CDC13) : # 1.22-1.45 (5H, m), 1.76-1.87 (5H, m), 2.02 (6H, 2xs), 2.56 (1H, m), 3.22-3.23 (4H, t), 3.29-3.30 (4H, t), 3.88 (3H, s), 6.86 (1H, d), 6.94 (1H, dd), 7.30 (1H, d), 7.59 (1H, d), 7.79 (2H, d), 7.98 (1H, s) and 8. 54 (4H, bs); MS (AP+ve): n2/z 408 [M+H+3.

The following tables give Examples which illustrate but do not limit the invention in any way.

Table A Encompassing compounds of general formula (II), a subset of formula (I) where A = H and OMe, R3 = H, X = O, Y = CH2CH2, Z = a bond ; R4 = Ph and R5 is either meta or para substituted on R4. Example R5 pI meta/ [M+H] + Procedure No. N para -R2 Al Ph p 447 A7 :. ON A2 O N P 453 A7 /\N A3 p 437 A7 ;. o--N A4 Ph m 447 A7 O-N p 448 A7 A5 con A6 p 489 A7 /\ ;. owN A7 p 461 A7 A8 p 453 A7 A8 p 453 A7 U.-oY A9 p 453 A7 ___ _,, o .''-1' A10 N p 451 A7 0,/-N All p 529 A7 N-".-0- ! A12 p 461 A7 .. ON A13 472 A7 ... w vN :. O'N A14 p 525 A7 So-, o/--N A15 m 453 A7 _ : ON S A16 s m 453 A7 ;. O"N/ A17 0 p 489 A7 A17 O ____ -p 489 A7 t A18 Now p 486 A7 N \ A19 N-N _ -p 529 A7 A20 U O Nf 453 A7 ____ ;. ON A21 N p 449 A7 \==N-"o" A22 ° 477 A22 A23 p 515 A22 ,. O_"'N FF FF A24 p 462 A22 J.-o-N A25/r"\\-'P 553 A22 A25 p 553 A22 ) =% w ___ A26 p 497 A22 : ON A27 p 497 A22 <-, o N A28 p 461 A22 _ : ON A29/==\ + P 493 A22 A30 p 515 A22 Ft 3 O Nf I A31 475 A22 _ :. ON A32 0 p 491 A22 '.'_'_,,, o A33/=\\-p 473 A22 <-, o N A34 p 477 A22 o-O A35 Ph P 433 A51 :. ON A36/o0 439 A51 ___ A37 Ph p 397 A51 ,,. 0/'- A38 p 391 A51 ___,,, o A39/--\ (p 423 A51 0 0 N A40 Ph) p 417 A51 O/,'en l- ! A41 Ph p 417 A51 . A42 p 423 A51 ___ O N\ A43 Ph ! p 405 A51 oY" A44 p 411 A51 0 A45 Ph p 419 A51 . _O-'N A46 p 425 A51 \/0" " A47 Ph p 417 A51 A48 N p 423 A51 A4S/--\N P'-' ---°y A49 Ph -Ph p 467 A51 0 A50/-Ph p 473 A51 _-_,,, o A51 Ph p 417 A51 O- A52 p 423 A51 zozo O-N A53 0 p 421 A22 -.,. o"\ A54 Ph woO nNs 405 A51 A55 _-_,, O N/p 411 A51 A56 Ph p 431 A51 A57 0 p 437 A51 A57 p 437 A51 N A58 Ph o NC P 445 A51 ., p-N A59 P 451 A51 ---"vj A60 H2N/406 A60 0 ,,. o A61 Ph"",/p 497 A63 ,,. ON A62 p 459 A63 ,,, 0"N \ A63 p 419 A63 HOC Hoc \ A64. N p 417 A63 A65 o"0 N 421 A63 ,,. o/--N o A66 p 441 A63 CO-°-< A67 p 441 A63 UU-"° A68 p 404 A63 ,,. o--N A69 p 437 A63 J0" s A70 p 434 A63 o : o O A71 p 459 A63 Foc F3C A72 Ph 04 X P 481 A51 k-N A73 O--''w \ p 487 A51 zu A74 Ph p 445 A51 A75 ° \/NtO p 451 A51 \N-0 A76 Ph p 493 A51 0-N A77 p 499 A51 9/\- A78 Ph \ P 479 A51 i A79 p 485 A51 ole A80 Ph o N p 508 A51 q, N, Ph A81 rN"-) p 514 A51 N. oh A82 Ph, 09 P 403 A51 N N A83 X AN\ 1 » 409 A51 ' LJY-N A84 p 449 A84 .. ON O A85 p 445 A88 A86 p 487 A88 F3c,, o F3C A87 p 425 A88 ,. O"N A88 p 450 A88 0 N 0, I A89 ri-1"p 450 ASS N o A90 p 459 A88 cl-ou A91 S ONv 425 A88 A92 p 458 A88 -N A93 p 447 A93 0 A94 p 443 A93 A95 P 485 A93 Foc E Fs A96'N\----p 423 A93 \ORS A97 p 431 A93 A98 p 448 A93 0"Nj 0 N A99 p 431 A93 A100 p 479 A93 0)'na' A101 p 457 A93 0 A102 p 423 A93 S'".'0- A103 p 407 A93 O __ A104 p 459 A93 . A105 Ph p 429 A105 A106 Ca O N. 426 A107 .. oNV/ A107 Grv''' p 424 A107 ,, p--N A108 p 454 A107 ka/"

Table B Encompassing compounds of general formula (III), a subset of formula (1) where A = H and OMe, Rl = R2 = Me2, R3 = H, X = O, Y = CH2-CH2, Z = O, CH2 or NH; R4 = Ph, R5 is Ph and Z is either meta or para substituted on R4.

(III) Example Z meta//1 [M+H] + Procedure No. para . NR2 Bl O 0 m 463 Bi l B2 CH2 P) ~ 461 B1 , OorN ,,. ON O"o>/<N// B4 CH2 P/s0 4447 A51 "o>/N// B5 O m/407 A51 "Os/<Ns B6 CH2 p 405 A51 "Oo/iNs B7 O m 433 A51 , B8 CH2 p 431 A51 . O B9 O m 433 A51 "10 N B10 CH2 p/431 A51 "0 N Bll 0 m 421 A51 ,,. 0 B12 CH2 p/419 A51 ,,. 0 B13 O m/-435 A51 0" B14 CH2 p/-433 A51 0/\ B 15 O m 433 A51 ,,. B16 CH2 p 431 A51 .-o- B17 O m Ph 483 A51 .. o B18 CH2 p Ph 481 A51 0"\ B19 O m 433 A51 .-p-N B20 CH2 p 431 A51 .-0- B21 CH2 po/419 A51 N B22 O m 447 A51 ', p--N B23 CH2 p 445 A51 "10,,/-N B24 O m/497 A51 ,,, o,/-N h B25 CH2 p 495 A51 pu B26 O m 509 A51 9/\-N B27 CH2 p 507 A51 /\-N B28 O m O-NX 495 A51 B29 CH2 p 493 A51 cl B30 O m 524 A51 N-Ph B31 CH2 p,-N 522 A51 -Ph B32 O m 419 A51 V B33 CH2 p,. 0. . N 417 A51 B34 CH2 m I 461 Bl ;, ONJ\ B35 O p 463 Bl 0 B36 NH p 462 B37 ,.'OM B37 NH p 432 B37 . : ON Table C

Encompassing compounds of general formula (IV) a subset of formula (1) where A = H and OMe, RI = R2 = Me2, R3 = H, X = O, Y = CH2-CH2 ; R4, R5 = substituted phenyl or heterocycle, (IV) Example Z 3/4 0 R5 [M+H] + Method No. substitution w. r. t. C=O R4 Ci bond 4 0 Ph 461 Ci / C2 bond 4 3 Ph 477 Ci I C3 bond 4 Ph 461 Cl I i C4 bond 3 @ Ph 453 C1 fez C5 O 3 0 521, ci 523, 525 , O ci C6 bond 3 nix Ph 451 Ci , NJ\ C7 bond 4 Ph 448 Ci 'N C8 bond 4 C'P Ph 481,483 Cl C9 bond ; Fsq c 4 539, C1 o"° r. ' 541 C10 bond 3, 0 539 ci CF3 CI Cil bond 3 0 453 Cl zon , S C12bond39"/525Cl I _ CF__

Table D Encompassing compounds of general formula (V) a subset of formula (I) where R3 = H, X = O, Y = CH2-CH2, Z = O, CH2, NH or a bond ; R4 = Ph, R5 is Ph or cyclohexyl (Cy) (V) Example Z R6 R7 R5 meta [M+H] Method No. + para'"r<2 D1 bond ci H Ph p \452, Dl 454 D2 O ci H Ph m, o N/468, D1 470 D3 CH2 ci H Ph p 466, DI 468 D4 bond ci H Cy o N/460 D1 460 D5 bond H ci Ph p 452, D5 454 D6 O H Cl Ph m 468, D5 470 D7 CH2 H ci Ph p \ 466, D5 468 D8 bond H Cl Cy p 458, DS 460 D9 bond F H Ph p 435 D9 .. ON DIG CH2 F H Ph p \ 449 D9 l Dll bond F H Ph p 441 D9 .. ON D12 bond H F Ph p 435 D12 .. 0-N D13 O H F Ph m 0 M/451 D12 O"' D14CH2HFPh p\ 449 D12 .. ON D15 bond H F Cy p \ 441 D12 .. ON D16 bond Me H Ph p 431 D16 .. ON D17 O Me H Ph m 447 D16 ... 0-N D18 CH2 Me H Ph P o N 445 D16 ,. 0-N D19 bond Me H Cy p 437 D16 .. ON D20 bond H Me Ph p 431 D20 .. ON D21 O H Me Ph m 447 D20 .. ON D22 CH2 H Me Ph p 445 D20 .. ON D23 bond H Me Cy p 437 D20 .. ON D24 bond COCH3 H Ph p r"431 D24 D25 bond OMe CHO Ph p 475 D25 .. ON D26 bond cH (O) CH3 H Ph p 433 D26 .. ON D27 bond Et H Ph p 417 D27 ,. 0-N D28 bond H H Ph p 417 D28 .. C ? '_"N D29 O H H Ph m O N 433 D28 .. ON D30 bond H H Ph p/361 D30 ,, O. Nw D31 O H H Ph p 433 D28 0-N D32 O H H Ph p 405 D32 0/-' D33 O H H Ph m 405 D32 .. oN/ D34 bond H H Cy p 423 D28 / D35 bond H H Cy p 395 D32 D36 CH2 H H Ph p 431 D28 / .. ON I D37 CH2 H H Ph r N 403 D32 D38 bond H H p-p -445 D38 EtPh D39 bond H H p 417 D39 EtPh,, p---N Table E

Encompassing compounds of general formula (VI) a subset of formula (1) where A = H, Cl, F and OMe, X = O, Y = CH2-CH2 ; R4 = phenyl, R5 = phenyl or cyclohexyl (Cy), Z = O, CH2 or a bond Example Z o/p R3 R8 R9 RS ~ sR2 M+H] Method No. + E1 bond p Me H MeO Ph 461 El E2 O m Me H Me0 Ph o +)/4 El .. ON E4 CH2 p Me H MeO Ph Y-475 El N I ES bond p Me H MeO Cy 467 El :. ON T E6 bond p Et H MeO Ph o N 447 E1 E7 bond p Et H MeO Ph 445 El .. ONJ E8 bond p Me H MeO Ph 431 El .. ONJ E9 bond p Me H MeO Ph 433 El E10 bond p Et H MeO Cy 453 E1 Ell bond p Et H MeO Cy 451 El .-O- E12 bond p Me Cl MeO Ph 468, E12 ,-'N/470 E13 bond P Me H MeO 2-F-Ph 451 E13 "o N E14 bond p Me H MeO 2-Me-r 447 E14 Ph E15 bond p Me H MeO 2-463 E14 MeO- Ph E16 bond p Me H MeO 2-Cl-Ph 468, E14 470 E17 bond p Me H MeO 4-F-Ph r 451 E14 "O N E18 bond p Me H MeO 4-F3C-501 E14 Ph E19 bond p Me H MeO 4-Me-r 447 E14 Ph E20 bond p Me H MeO 4-463 E14 MeO- Ph E21 bond p Me H MeO 4-Cl-Ph 468, E14 "o N 470 E22 bond p Me H MeO 4-Et-Ph 461 E14 "O N E23 bond p Me H MeO 4tBu-489 E14 Ph E24 bond p Me F MeO Ph/""'451 E24 "o N E25 bond p Et H Me Ph 459 E25 "o N< E26 bond p Et H Me Cy 465 E25 , o N< E27 CH2 P Et H Me Ph 473 E25 : Table F

Encompassing compounds of general formula (VII) a subset of formula (1) where A = H and OMe, X = O, R4 = 3-pyridyl, R5 = phenyl, Z = a para bond R1 <BR> <BR> <BR> 0 # O N # R2<BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> I<BR> N#N# O<BR> <BR> <BR> <BR> # H<BR> <BR> <BR> <BR> #<BR> <BR> <BR> <BR> <BR> <BR> # (VII) Example F1 [M+Hl+ Method No. "%2 F1 Rl = R2 = Me 392 F1 F2 \ 418 Fl : O N F3 418 Fl F4 + 448 F1 "o Nt Table G

Encompassing compounds of general formula (VIII) a subset of formula (I) where A = H and OMe, R3 = H, X = 0 ; R4 = phenyl, Z = O, CH2 or a bond and R5 = Ph or cyclohexyl (Cy), Y is a chain of 3 or 4 carbon atoms optionally substituted by an hydroxyl group.

(VIII) Example Z m/R5 XYN F {1 [M+H] + Method No. No. p'R2 G1 bond P Ph o N"Ns/449 G1 oh G2 bond p Ph o N", NC 461 G1 oh G3 bond Ph Ph oN r, N 476 Gl OH Oh G4 bond p Ph o N"NX. 476 G1 OH G5 bond p Ph 465 G5 OH OH G6 bond p Ph 0--N 475 GI OH G7 bond p Ph u~ t1 475 (l OH G8 bond P Cy OH +, N/3-a OH G9 bond Ph Ph Nr3 447 GS OH G10 bond p Cy oN '455 G8 ou OH Gil bond p Ph oN '449 G8 OH G12 bondp Cy 0 N 483 G5, G8 OH OH<- G13 bond p Ph oN 477 GS, G8 OH OH< G14 bond p Cy C) N 482 G8 oh G15 bondp Ph C) N 476 G8 oh OU G16 bond p Cy oN r' 481 G8 C) H Oh G17 bond p Cy oN 481 G8 OH G18 bond p Ph O<N, G8 NU OH G19 bond p Ph O<N"NX 475 G8 Oh OH-/ G20 bond p Ph oN J 444 G8, G5 OH ND- OH G21 bond p Ph 0 461 G8 OH G22 bond P Ph 0,,, N NMe2 419 G22 G23 O m Ph oz, N N Me2 435 G22 G24 CH2 p Ph o%'/N NMe2 433 G22 G25 bond p Cy o%'//N NMe2 425 G22

Table H Encompassing compounds of general formula (IX) a subset of formula (I) where A = H and OMe, R3 = H, X = N ; R4 = phenyl, Z = a para substituted bond and R5 = Ph or cyclohexyl (Cy), Y and R2 form a piperazinyl ring between X and N.

(IX) Example R5 Rl [M+H] + Method No. H1 Cy Me 408 H1 H2 Cy Et 436 HI H3 Cy iPr 422 H1

The activity of the compounds used in this invention may be assessed by competitive binding assays to 1 lCBy receptors, as follows: Radioligand Binding Studies Radioligand binding assays were carried out on well washed membranes from HEK293 cells stably expressing IlCBy receptors. Membranes (5-15 mg protein) were incubated with [125I]-Melanin Concentrating Hormone (0.22 nM) (obtained from NEN) in the presence and absence of competing test compounds for 45 min at 37°C in a buffer (pH7.4), containing 50mM Tris and 0.2% BSA. Non-specific binding was defined using 0. 1 mM Melanin Concentrating Hormone (obtained from Bachem). The test compounds were added at concentrations between 10M and 10pM in 10 concentration steps.

Following incubation, the reaction was stopped by filtration through GF/B filters and

washed with 4 x lml of ice-cold 50mM Tris buffer. Microscint 20 (Packard) was added to the filters and the radioactivity measured using a Packard TopCount.

Bound cpm in the presence of test compound was expressed as a fraction of the bound cpm in the absence of test compound and plotted against the concentration of compound.

From this an IC50 was determined from which the pKi was calculated.

The most potent compounds of the present invention have pKi values in the range of 7.1 to 7. 8 For example : Example pKi range A48 7.5-7.8 B2 7.1-7.4 C8 7.1-7.4 D15 7.5-7.8 E9 7.5-7.8 F4 7.1-7.4 G1 7.1-7.4 H1 7.1-7.4