Title:
CASRX PREPARATION AND METHOD FOR SILENCING TARGET GENE
Document Type and Number:
WIPO Patent Application WO/2022/110260
Kind Code:
A1
Abstract:
A CasRx preparation and an application for silencing a target gene. The CasRx preparation comprises CasRx mRNA and sgRNA; the CasRx mRNA encodes a CasRx protein, and the amino acid sequence of the CasRx protein is shown in SEQ ID No. 1; and the sgRNA is set according to a target gene. Directly introducing the CasRx mRNA and the sgRNA for the target gene into a target cell makes up the blank of a CasRx-based efficient directional gene silencing system in the field of mRNA preparations, and solves the defect of a CasRx system carried by an AAV virus.
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Inventors:
HU YONG (CN)
ZHANG MIAOMIAO (CN)
HONG DAN (CN)
HU XUN (CN)
ZHANG MIAOMIAO (CN)
HONG DAN (CN)
HU XUN (CN)
Application Number:
PCT/CN2020/133300
Publication Date:
June 02, 2022
Filing Date:
December 02, 2020
Export Citation:
Assignee:
SHENZHEN RHEGEN BIOMEDICAL TECH CO LTD (CN)
International Classes:
C12N15/113; C12N9/22
Domestic Patent References:
| WO2019236982A1 | 2019-12-12 |
Foreign References:
| CN111328343A | 2020-06-23 | |||
| CN108138181A | 2018-06-08 | |||
| CN109097397A | 2018-12-28 | |||
| CN110804628A | 2020-02-18 |
Other References:
ZHOU CHANGYANG, HU XINDE, TANG CHENG, LIU WENJIA, WANG SHAORAN, ZHOU YINGSI, ZHAO QIMENG, BO QIYU, SHI LINYU, SUN XIAODONG, ZHOU H: "CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration", NATIONAL SCIENCE REVIEW, vol. 7, no. 5, 1 May 2020 (2020-05-01), pages 835 - 837, XP055933921, ISSN: 2095-5138, DOI: 10.1093/nsr/nwaa033
HE BINGBING, PENG WENBO, HUANG JIA, ZHANG HANG, ZHOU YINGSI, YANG XIALI, LIU JING, LI ZHIJIE, XU CHUNLONG, XUE MINGXING, YANG HUI,: "Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver", PROTEIN & CELL, SPRINGER ASIA, BEIJING, CN, vol. 11, no. 7, 1 July 2020 (2020-07-01), Beijing, CN , pages 518 - 524, XP055933923, ISSN: 1674-800X, DOI: 10.1007/s13238-020-00700-2
XU XIAOJIE, WAN TAO, XIN HUHU, LI DA, PAN HONGMING, WU JUN, PING YUAN: "Delivery of CRISPR/Cas9 for therapeutic genome editing", THE JOURNAL OF GENE MEDICINE, JOHN WILEY & SONS, INC, US, vol. 21, no. 7, 1 July 2019 (2019-07-01), US , XP055933925, ISSN: 1099-498X, DOI: 10.1002/jgm.3107
HE BINGBING, PENG WENBO, HUANG JIA, ZHANG HANG, ZHOU YINGSI, YANG XIALI, LIU JING, LI ZHIJIE, XU CHUNLONG, XUE MINGXING, YANG HUI,: "Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver", PROTEIN & CELL, SPRINGER ASIA, BEIJING, CN, vol. 11, no. 7, 1 July 2020 (2020-07-01), Beijing, CN , pages 518 - 524, XP055933923, ISSN: 1674-800X, DOI: 10.1007/s13238-020-00700-2
XU XIAOJIE, WAN TAO, XIN HUHU, LI DA, PAN HONGMING, WU JUN, PING YUAN: "Delivery of CRISPR/Cas9 for therapeutic genome editing", THE JOURNAL OF GENE MEDICINE, JOHN WILEY & SONS, INC, US, vol. 21, no. 7, 1 July 2019 (2019-07-01), US , XP055933925, ISSN: 1099-498X, DOI: 10.1002/jgm.3107
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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