Title:
CELL ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2021/153253
Kind Code:
A1
Abstract:
This cell analysis method comprises: a mixing step S11 for producing a mixed liquid by mixing a cell, which is an analyte, with a metal ion solution and a reducing agent; a metallic microstructure creation step S12 for creating a metallic microstructure on a support body by reducing the metal ions in the mixed liquid through the reducing action of the reducing agent in the mixed liquid and adhering the cell or a substance derived from the cell to the metallic microstructure; a drying step S13 for drying the support body after the metallic microstructure creation step; a measurement step S15 for irradiating excitation light onto the metallic microstructure on the support body after the drying step and measuring the Raman scattered light spectrum produced by the irradiation of the excitation light; and an analysis step S16 for analyzing the cell on the basis of the Raman scattered light spectrum. As a result, the present invention achieves a method that makes it possible to easily analyze cells as an analyte using highly efficient SERS spectroscopy.
Inventors:
FUJIWARA KAZUHIKO (JP)
KAZAMI SAYAKA (JP)
MARUYAMA YOSHIHIRO (JP)
KAZAMI SAYAKA (JP)
MARUYAMA YOSHIHIRO (JP)
Application Number:
PCT/JP2021/001046
Publication Date:
August 05, 2021
Filing Date:
January 14, 2021
Export Citation:
Assignee:
HAMAMATSU PHOTONICS KK (JP)
International Classes:
G01N21/65
Domestic Patent References:
| WO2017086318A1 | 2017-05-26 | |||
| WO2007060988A1 | 2007-05-31 |
Foreign References:
| JP2018025431A | 2018-02-15 | |||
| JP2006514309A | 2006-04-27 |
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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