Title:
CELL ANALYSIS METHODS, COMPOSITIONS, AND USES
Document Type and Number:
WIPO Patent Application WO/2023/283537
Kind Code:
A3
Abstract:
A chemical approach is provided to profile uptake of compounds in cells, alone or in combination with other analytical techniques. The disclosure is particularly well suited for detecting cellular uptake of metabolites such as fatty acids. The disclosure can be carried out, applied in solution or on a surface, in bulk or on single cells, and is compatible with one or more additional techniques such as protein analysis. The disclosure is exemplified by probing fatty acid influx alone and in combination with proteomics analysis on a single-cell barcode chip to identify a combination therapy for inhibiting fatty acid metabolism and treating cancer.
Inventors:
XUE MIN (US)
WEI WEI (US)
GUO ZHILI (US)
CHENG HANJUN (US)
LI ZHONGHAN (US)
WEI WEI (US)
GUO ZHILI (US)
CHENG HANJUN (US)
LI ZHONGHAN (US)
Application Number:
PCT/US2022/073378
Publication Date:
March 30, 2023
Filing Date:
July 01, 2022
Export Citation:
Assignee:
INST SYSTEMS BIOLOGY (US)
UNIV CALIFORNIA (US)
UNIV CALIFORNIA (US)
International Classes:
G01N33/547; B01J19/00; G01N21/64; G01N33/533; A61P35/00
Domestic Patent References:
| WO2021011895A1 | 2021-01-21 | |||
| WO2020214858A1 | 2020-10-22 | |||
| WO2020247448A1 | 2020-12-10 | |||
| WO2021035310A1 | 2021-03-04 |
Foreign References:
| US20100093553A1 | 2010-04-15 | |||
| US20200038871A1 | 2020-02-06 | |||
| US20160333398A1 | 2016-11-17 | |||
| US20190293666A1 | 2019-09-26 | |||
| US20090035792A1 | 2009-02-05 | |||
| US20190194709A1 | 2019-06-27 | |||
| US20190390266A1 | 2019-12-26 |
Other References:
SUN DE-EN, FAN XINQI, ZHANG HAO, HUANG ZHIMIN, TANG QI, LI WEI, BAI JINYI, LEI XIAOGUANG, CHEN XING: "Click-ExM enables expansion microscopy for all biomolecules", BIORXIV, 20 March 2020 (2020-03-20), pages 1 - 19, XP093060338, [retrieved on 20230703], DOI: 10.1101/2020.03.19.998039
GUO ZHILI, CHENG HANJUN, LI ZHONGHAN, SHAO SHIQUN, SARKAR PRIYANKA, WANG SIWEN, CHAUDHURI ROHIT, PERKINS NICOLE G., JI FEI, WEI WE: "Single-Cell Profiling of Fatty Acid Uptake Using Surface-Immobilized Dendrimers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 143, no. 29, 28 July 2021 (2021-07-28), pages 11191 - 11198, XP093060340, ISSN: 0002-7863, DOI: 10.1021/jacs.1c05103
GUO ZHILI, CHENG HANJUN, LI ZHONGHAN, SHAO SHIQUN, SARKAR PRIYANKA, WANG SIWEN, CHAUDHURI ROHIT, PERKINS NICOLE G., JI FEI, WEI WE: "Single-Cell Profiling of Fatty Acid Uptake Using Surface-Immobilized Dendrimers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 143, no. 29, 28 July 2021 (2021-07-28), pages 11191 - 11198, XP093060340, ISSN: 0002-7863, DOI: 10.1021/jacs.1c05103
Attorney, Agent or Firm:
GRAF, Susan W. et al. (US)
Download PDF:
Previous Patent: YTTRIA-ZIRCONIA SINTERED CERAMICS FOR PLASMA RESISTANT MATERIALS
Next Patent: FOLDABLE STEP STOOL WITH FOLD-BLOCKING LOCK
Next Patent: FOLDABLE STEP STOOL WITH FOLD-BLOCKING LOCK