CELL FREE MANUFACTURE OF CAROTENOIDS - DEBUT BIOTECHNOLOGY INC

Title:

CELL FREE MANUFACTURE OF CAROTENOIDS

Document Type and Number:

WIPO Patent Application WO/2023/122529

Kind Code:

A3

Abstract:

The present invention features a cell-free system to produce astaxanthin or an intermediate product from beta-carotene. The present invention utilizes beta-carotene in a reaction mixture comprising a beta-carotene hydroxylase enzyme (CrtZ), a beta-carotene ketolase enzyme (CrtW) or a combination thereof to produce astaxanthin.

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Inventors:

BRITTON JOSHUA (US)
BRIDEAU NICHOLAS (US)
WAGNER DREW (US)

Application Number:

PCT/US2022/081921

Publication Date:

November 30, 2023

Filing Date:

December 19, 2022

Export Citation:

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Assignee:

DEBUT BIOTECHNOLOGY INC (US)

International Classes:

C12N9/00; C12N9/10; C12N9/88; C12P23/00

Foreign References:

US20070157339A1

2007-07-05

Other References:

FRASER ET AL.: "In Vitro Characterization of Astaxanthin Biosynthetic Enzymes", J. BIOL. CHEM., vol. 272, no. 10, 7 March 1997 (1997-03-07), pages 6128 - 6135, XP002142175, DOI: 10.1074/jbc.272.10.6128

Attorney, Agent or Firm:

SCHOEN, Adam, M. et al. (US)

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