Title:
CELL-FREE TRANSLATION SYSTEM FOR THE PRODUCTION OF (CHEMICALLY) MODIFIED PROTEINS
Document Type and Number:
WIPO Patent Application WO2006082058
Kind Code:
A3
Abstract:
The present invention relates to a ceil-free translation system comprising a nonsense-codon suppressing agent and an anti-release factor antibody which precipitates an/ or crosslinks a release factor in said cell-free translation system. The present invention further relates to a kit comprising said cell free translation system. Moreover, the present invention relates to an anti-release factor antibody directed against release factor of Thermus thermophiles. Furthermore, the present invention relates to the use of an anti-release factor antibody for the preparation of a celi-free translation system. Moreover, the present invention relates to a method for the production of an anti-release factor antibody. Furthermore, the present invention provides for a method for the production of alloproteins. Further, the present invention relates to a puromycin derivative having a residue covalently attached directly or via a linker to one of its elements selected from the group consisting of the element N4 of the cytosine-residue of an 51 attached cytidine-residue and the element C5 of the cytosine-residue of an 5' attached cytidine-residue. Moreover, the present invention relates to the use of said puromycin derivative in the cell-free translation system of the present invention, in the method for the production of alloproteins of the present invention. The invention also provides for specific kits described herein.
Inventors:
SPRINZL MATHIAS (DE)
AGAFONOV DMITRY (DE)
RABE KERSTEN (DE)
AGAFONOV DMITRY (DE)
RABE KERSTEN (DE)
Application Number:
PCT/EP2006/000926
Publication Date:
November 09, 2006
Filing Date:
February 02, 2006
Export Citation:
Assignee:
UNIV BAYREUTH (DE)
SPRINZL MATHIAS (DE)
AGAFONOV DMITRY (DE)
RABE KERSTEN (DE)
SPRINZL MATHIAS (DE)
AGAFONOV DMITRY (DE)
RABE KERSTEN (DE)
International Classes:
C12P21/02; C07H19/16; C07H21/02; C12N9/18
Foreign References:
| EP1350846A1 | 2003-10-08 | |||
| US5405950A | 1995-04-11 | |||
| US4828979A | 1989-05-09 |
Other References:
AGAFONOV D E ET AL: "Efficient suppression of the amber codon in E. coli in vitro translation system", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 579, no. 10, 11 April 2005 (2005-04-11), pages 2156 - 2160, XP004850720, ISSN: 0014-5793
AGAFONOV D E ET AL: "C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1", CHEMBIOCHEM 2006 GERMANY, vol. 7, no. 2, 2006, pages 330 - 336, XP002385501, ISSN: 1439-4227 1439-7633
MIYAMOTO-SATO E ET AL: "SPECIFIC BONDING OF PUROMYCIN TO FULL-LENGTH PROTEIN AT THE C-TERMINUS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 5, 2000, pages 1176 - 1182, XP002908838, ISSN: 0305-1048
SZKARADKIEWICZ K ET AL: "RNA aptamers directed against release factor 1 from Thermus thermophilus", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 514, no. 1, 6 March 2002 (2002-03-06), pages 90 - 95, XP004597956, ISSN: 0014-5793
SHORT GLENN F III ET AL: "Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA, US, vol. 38, no. 27, 6 July 1999 (1999-07-06), pages 8808 - 8819, XP002195367, ISSN: 0006-2960
SHIMIZU Y ET AL: "CELL-FREE TRANSLATION RECONSTITUTED WITH PURIFIED COMPONENTS", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 19, August 2001 (2001-08-01), pages 751 - 755, XP001060378, ISSN: 1087-0156
DOI NOBUHIDE ET AL: "Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions", GENOME RESEARCH, vol. 12, no. 3, March 2002 (2002-03-01), pages 487 - 492, XP002394791, ISSN: 1088-9051
AGAFONOV D E ET AL: "C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1", CHEMBIOCHEM 2006 GERMANY, vol. 7, no. 2, 2006, pages 330 - 336, XP002385501, ISSN: 1439-4227 1439-7633
MIYAMOTO-SATO E ET AL: "SPECIFIC BONDING OF PUROMYCIN TO FULL-LENGTH PROTEIN AT THE C-TERMINUS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 5, 2000, pages 1176 - 1182, XP002908838, ISSN: 0305-1048
SZKARADKIEWICZ K ET AL: "RNA aptamers directed against release factor 1 from Thermus thermophilus", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 514, no. 1, 6 March 2002 (2002-03-06), pages 90 - 95, XP004597956, ISSN: 0014-5793
SHORT GLENN F III ET AL: "Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA, US, vol. 38, no. 27, 6 July 1999 (1999-07-06), pages 8808 - 8819, XP002195367, ISSN: 0006-2960
SHIMIZU Y ET AL: "CELL-FREE TRANSLATION RECONSTITUTED WITH PURIFIED COMPONENTS", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 19, August 2001 (2001-08-01), pages 751 - 755, XP001060378, ISSN: 1087-0156
DOI NOBUHIDE ET AL: "Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions", GENOME RESEARCH, vol. 12, no. 3, March 2002 (2002-03-01), pages 487 - 492, XP002394791, ISSN: 1088-9051
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