Title:
CELL LABELLING METHOD
Document Type and Number:
WIPO Patent Application WO/2023/149585
Kind Code:
A1
Abstract:
The present invention pertains to a method for labelling a cell using a bar code. A method for labelling a cell using a bar code according to the present invention comprises (1) bringing, in a cell population including a plurality of single cells, a modified bar code directly or indirectly into contact with a cell surface protein of the cells, or (2) bringing a modified bar code directly or indirectly into contact with a cell surface protein of a single cell.
Inventors:
SUGIMOTO MICHIHIKO (JP)
ABE KUNIYA (JP)
ABE KUNIYA (JP)
Application Number:
PCT/JP2023/004476
Publication Date:
August 10, 2023
Filing Date:
February 02, 2023
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12Q1/02; C12Q1/6816; C12N15/11
Domestic Patent References:
| WO2020132087A1 | 2020-06-25 |
Other References:
LATB STAFF: "Summary of biotin labeling reagents | What you need to know! Protein Experiments Part 11", THERMO FISHER SCIENTIFIC, 4 March 2020 (2020-03-04), XP093083672, Retrieved from the Internet
SUGIMOTO MICHIHIKO, TADA YUHKI, SHICHINO SHIGEYUKI, KOYAMATSU SAEKO, TSUMAKI NORIYUKI, ABE KUNIYA: "Universal Surface Biotinylation: a simple, versatile and cost-effective sample multiplexing method for single-cell RNA-seq analysis", DNA RESEARCH, UNIVERSAL ACADEMY PRESS, JP, vol. 29, no. 3, 27 May 2022 (2022-05-27), JP , XP093083675, ISSN: 1340-2838, DOI: 10.1093/dnares/dsac017
SUGIMOTO MICHIHIKO, TADA YUHKI, SHICHINO SHIGEYUKI, KOYAMATSU SAEKO, TSUMAKI NORIYUKI, ABE KUNIYA: "Universal Surface Biotinylation: a simple, versatile and cost-effective sample multiplexing method for single-cell RNA-seq analysis", DNA RESEARCH, UNIVERSAL ACADEMY PRESS, JP, vol. 29, no. 3, 27 May 2022 (2022-05-27), JP , XP093083675, ISSN: 1340-2838, DOI: 10.1093/dnares/dsac017
Attorney, Agent or Firm:
YAMAMOTO, Osamu et al. (JP)
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