Title:
CELL LYSIS MONITORING METHOD, AND METHOD FOR PRODUCING POLYHYDROXYALKANOATES BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2023/121396
Kind Code:
A1
Abstract:
The present invention relates to a method for producing polyhydroxyalkanoates (PHAs), and a cell lysis monitoring method, and provides a method for producing polyhydroxyalkanoates (PHAs), the method allowing the length and sphericity of cells or PHAs to be measured through three-dimensional image analysis using the difference in refractive index of cells or PHAs, so that the autolysis degree, input status, or lysis degree of cells of PHAs is identified, and thus optimum lysis conditions are set and the stability of an impurity removal process, which is a back-end process of a lysis process, can be increased.
Inventors:
KIM DA EUN (KR)
SEO DONG JUNE (KR)
PARK JOO HYUN (KR)
KIM KA-YEONG (KR)
SEO DONG JUNE (KR)
PARK JOO HYUN (KR)
KIM KA-YEONG (KR)
Application Number:
PCT/KR2022/021206
Publication Date:
June 29, 2023
Filing Date:
December 23, 2022
Export Citation:
Assignee:
CJ CHEILJEDANG CORP (KR)
International Classes:
G01N33/483; C12P7/62; G01N21/17; G01N21/31
Foreign References:
| JP2018528235A | 2018-09-27 | |||
| US20120252081A1 | 2012-10-04 |
Other References:
HWANG KUO-JEN, YOU SHAO-FU, DON TRONG-MING: "Disruption Kinetics of Bacterial Cells during Purification of Poly-β -Hydroxyalkanoate Using Ultrasonication", JOURNAL OF THE CHINESE INSTITUTE OF CHEMICAL ENGINEERS, TAIPEI, TW, vol. 37, no. 3, 1 January 2006 (2006-01-01), TW , pages 1 - 8, XP093073674, ISSN: 0368-1653
CHOI SO YOUNG, OH JEONGHUN, JUNG JAEHWANG, PARK YONGKEUN, LEE SANG YUP: "Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 118, no. 31, 3 August 2021 (2021-08-03), XP093073681, ISSN: 0027-8424, DOI: 10.1073/pnas.2103956118
MOJAVERYAZDI FARZANEH SABBAGH, MUHAMAD IDA IDAYU, REZANIA SHAHABALDIN, BENHAM HIRO: "Importance of Glucose and Pseudomonas in Producing Degradable Plastics", JURNAL TEKNOLOGI, PENERBIT UNIVERSITI TEKNOLOGI MALAYSIA, MALAYSIA, vol. 69, no. 5, 1 January 2013 (2013-01-01), Malaysia , pages 7 - 10, XP093073692, ISSN: 0127-9696, DOI: 10.11113/jt.v69.3194
CHOI SO YOUNG, OH JEONGHUN, JUNG JAEHWANG, PARK YONGKEUN, LEE SANG YUP: "Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 118, no. 31, 3 August 2021 (2021-08-03), XP093073681, ISSN: 0027-8424, DOI: 10.1073/pnas.2103956118
MOJAVERYAZDI FARZANEH SABBAGH, MUHAMAD IDA IDAYU, REZANIA SHAHABALDIN, BENHAM HIRO: "Importance of Glucose and Pseudomonas in Producing Degradable Plastics", JURNAL TEKNOLOGI, PENERBIT UNIVERSITI TEKNOLOGI MALAYSIA, MALAYSIA, vol. 69, no. 5, 1 January 2013 (2013-01-01), Malaysia , pages 7 - 10, XP093073692, ISSN: 0127-9696, DOI: 10.11113/jt.v69.3194
Attorney, Agent or Firm:
FIRSTLAW P.C. (KR)
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