Title:
CELL MEASURING METHOD
Document Type and Number:
WIPO Patent Application WO/2006/062131
Kind Code:
A1
Abstract:
A technology capable of measuring cell-related reactions at a time within a wide range of concentration. Cell groups (200) are disposed in a cell-storing space (110) filled with liquid (120). A specific substance is supplied to the liquid in the cell-storing space (110) to form in the liquid (120) the concentration gradient (131) of the specific substance (130) extending from one end (111) of the cell-storing space (110) toward the other end (112). The cell groups in the cell-storing space (110) is picked up by an imaging device (320) and captured into an information processor, and image obtained by the information processor is divided into a plurality of regions along the above concentration gradient, feature amounts are extracted from image portions in respective regions, and information indicating the interrelation between the extracted feature amounts and the concentration gradient is created.
Inventors:
NITTA NAO (JP)
Application Number:
PCT/JP2005/022465
Publication Date:
June 15, 2006
Filing Date:
December 07, 2005
Export Citation:
Assignee:
EFFECTOR CELL INST INC (JP)
NITTA NAO (JP)
NITTA NAO (JP)
International Classes:
G01N15/14; C12Q1/02; G01N33/48
Domestic Patent References:
| WO2003091730A1 | 2003-11-06 |
Foreign References:
| JP2004003965A | 2004-01-08 | |||
| JP2002521690A | 2002-07-16 | |||
| JP2003088396A | 2003-03-25 |
Other References:
AOKI Y. ET AL: "Saibo Sokasei Sokutei Sochi TAXIScan o Mochiita Mast Saibo Datsu Karyu Hanno Kaisekiho no Kaihatsu", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SCIENTY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 27TH, 25 November 2004 (2004-11-25), pages 1026, 3PB-593, XP003000425
NITTA N. ET AL: "Gazo Kaiseki ni yoru TAXIScan Nai Saibo Sokasei no Teiryoka", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SCIENTY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 27TH, 25 November 2004 (2004-11-25), pages 346, W1R-2, XP003000426
LIU Z. ET AL: "A method for Measuring Bacterial Chemotaxis Parameters in a Microcapillary", BIOTECHNOLOGY AND BIOENGINEERING, vol. 51, no. 1, 1996, pages 120 - 125, XP003000427
FISHER P.R. ET AL.: "Quantitative analysis of cell motility and chemotaxis in Dictyostelium discoideum by using an image processing system and a novel chemotaxis chamber providing stationary chemical gradients", THE JOURNAL OF CELL BIOLOGY, vol. 108, no. 3, 1989, pages 973 - 984, XP003000428
NITTA N. ET AL: "Gazo Kaiseki ni yoru TAXIScan Nai Saibo Sokasei no Teiryoka", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SCIENTY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 27TH, 25 November 2004 (2004-11-25), pages 346, W1R-2, XP003000426
LIU Z. ET AL: "A method for Measuring Bacterial Chemotaxis Parameters in a Microcapillary", BIOTECHNOLOGY AND BIOENGINEERING, vol. 51, no. 1, 1996, pages 120 - 125, XP003000427
FISHER P.R. ET AL.: "Quantitative analysis of cell motility and chemotaxis in Dictyostelium discoideum by using an image processing system and a novel chemotaxis chamber providing stationary chemical gradients", THE JOURNAL OF CELL BIOLOGY, vol. 108, no. 3, 1989, pages 973 - 984, XP003000428
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY SHOWYOU INTERNATIONAL (9-10 Kitasaiwai 2-chome, Nishi-k, Yokohama-shi Kanagawa 04, JP)
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